smg1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	smg1
Entrez Gene ID	101066341
Full Name	SMG1, nonsense mediated mRNA decay associated PI3K related kinase
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029839736.1	XP_029695596.1	serine/threonine-protein kinase SMG1 isoform X2
XM_011606029.1	XP_011604331.1	LOW QUALITY PROTEIN: serine/threonine-protein kinase SMG1
XM_029839735.1	XP_029695595.1	serine/threonine-protein kinase SMG1 isoform X1

Pathways from BioSystems
Gene Ontology

The following smg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo22481	XM_011606029.1 Latest version!	Takifugu rubripes SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo70534	XM_029839735.1 Latest version!	Takifugu rubripes SMG1 nonsense mediated mRNA decay associated PI3K related kinase (smg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo70535	XM_029839736.1 Latest version!	Takifugu rubripes SMG1 nonsense mediated mRNA decay associated PI3K related kinase (smg1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smg1 ( XM_011606029.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604331.1 Takifugu rubripes SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), mRNA.

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CloneID	OTo22481
Clone ID Related Accession (Same CDS sequence)	XM_011606029.1
Accession Version	XM_011606029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	LOW QUALITY PROTEIN: serine/threonine-protein kinase SMG1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070993.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel internal stop codons :: corrected 2 genomic stop codon ##RefSeq-Attributes-END##

ATGAGCCGTA AATCTTCGGG GTCTCGGCTC AGCGGCGCCC ACAAACTGCA GCCACACTGG 
AATGACTGGC AGCCCAGAAC TGACAGTGCG TCACTCAGCC AGGAGGGAGA GCTCGATTCA 
TCCATATTGT ATGATATATT GCCCTCTAGC TGTCCCGATC CTCCAGTGGT GTCTAGCAAT 
GCCTTTCATG CTGCAGATAC ACACCAGGAA GAGGGTGGAT ATGAAGGCAA CAAAGTTATC 
TGTATTAATG CGTTTGGTTG GAAGTCGGTG GGGCAAGAGC TGAGGCCAAA TGACTTGACC 
ACAGATTTGC CTCGTTTTCA GCATGGAAAT CGTGCTTTAG CTTCAAAGGA CATGAAAAAA 
TCGCAGGACA GAGGAATGGG TCATTCAGAG GAGTCTCGGC TGGCCAACCT CCTGCGTCGA 
GTTTCTAGGG AGGATGATCG TTAAATAAAT ATTCTTTTTT TTTTTCCTCC CCTCTCTTAG 
ACACTGGTTA AACAATTGGA CACAATCCTC AGCACCCTAA ATGATATTTT AAATGAGAGT 
AGTAAGCTAC TCTGGGAGCT GCGTCAGGAT GCAGCCATTT GCCTTGGTCT ACTCTGCACG 
GTTCTCAGCT ATGAGGCTGA GAGCATCTTC AAGTGGCTAT TTCTAAAATT AACATCGAGC 
AATCGCGATG AAGTGAAGCT GTTGTACCTT ATGGCAACAT ATCACGCATT GGAGGCTGCT 
GGAGAGAGGA AGGCCTTTTC TCACGTCATG CAGCTGGTGA TGACCAATTT GCAGTCCATC 
CTGGAGAATG TTGACACACC AGAGCTGCTT TGTCAGAGTG TGAAGTGTAT TCTTCAGGTG 
GCGCACTGTT ACCCACATGT GTTCAGCATC AACTTTAGAG ATACAGTTGA TATATTGGTG 
GGCTGGCACA TAGACCACAC TCAGAAGCAG TCGGTCACAC AGCAAGTTTC AGGTTGGCTT 
CAGAGTCTGG AACAGTTCTG GGTGGCGGAC CTGACATTCT CCACCACACT ACTGGGACAG 
TTCCTGGAGG ATATGGAAGC ATATGCAGAG GATCTGAACC ATGTTGTTTT AGGGGAGATT 
CTCGATGAAG ATATTCCACC TCCCTCGGTG TCACTGCCAA AGTTAGCAGC ACTGTTGAGA 
GTTTTCAGCA CAGTGGTCCG CAGCATTGGA GAGCGATTTA GTCCCCTAAG AGGACCACCC 
ATTACGGAGG TCTACGTCAC AGATGTTCTG AACCGTGTTC TGGCCTGTGT GTCGACGGCA 
AAGCAGGTTC AGTTTTCTGA AGAAGTTCTC ACTGCTGGAA ACGAGTGTGT GGGTGTGTTG 
CTAGCTAGTG TGGAACCCAG TGGTCAGTTG ATGGAGGCTG TCTTGGTCTA TGGGCTGGAC 
CAGTTGGACT GCTGCCAGGC CTGCAGCTCA GACTACAACC TTTCAGTGCT CATCATCCTC 
ACTATGATAG TGGACCAAAT CAACACAAAG CTCCCAGCAT CATTTGTTGA AAAACTGTTG 
GCTCCAGAAT CCAAGTTGAT GCAGTTGCGG TTTCACAGAG ATAAAGAGGT GATTTCAGCT 
GTTCATGGTG TGTATCAGGC ATTGTTAAGC CTGAAGAACA TTCCTACACT TGAAGCAGCA 
TATAAAATGG TATTGGGTGA GATAGCATGT GCCCTCTCCA GCCTAACTTT GGTGCTGAAA 
GCCAATGGTA TGGCTCCTGC CCCCAATGGC TGCCTCTACT CAAATATCCA ACATCCTGCC 
TTTACTTCTC TCACCCTACC TTTGGAAAAT GCAGAGTTCA CACTGATCTT CAACCTCAGC 
ACCCTCACTA CCATTGGTAA CACCAAGAAT TCTCTGATTG GGATGTGGGC CTTGTCTCCT 
ACAGTCTTCA CACTGCTCAG TCAAAATCTT TTGTTGGTCC ACTCTGACTT AGCAGTGTAC 
TACCCCACCA TCCAGTATGC CGTACTTTAC ACACTGTACT CCCACTGCAC AAGACATGAT 
CATTTCATCT CATCCAGCTT ATCATCTTCT CCATCACTAT TTGATGGTGC TGTTATCAGC 
ACTGTGACCA CAGCAACAAA GAAACACTTC AGCACACTCT TAGGCCTTCT TGGAGGTCTC 
CTGTTGAAAG AACATCTCTA CATTGAGACC AGGAGACTGC TGTTGACCTG GGCTCAGGAA 
ATCTCGGTGC TGATGAAAAA GTCAGACACT TATAGCCCTC TCTTCTCTTT GACCACTTTC 
AACAAGTTTT GCAGGGGTCT CCTTGCCAAT GCTCTAAATG AAGATCAGAG TATTTGTCTT 
CAGACCTGCC ACTGTATTAT GGTTCTGTCT TCATCTTTGT CCACTGAGCT GCTGCAGAGC 
TGTGTGGATG TGTGTAGAGT ACAGTTGGTC AACTCAGCAG TAAGAGTAAG GCAGGCCTAT 
GGTAAGCTGC TCAAGACCAT TCCACTGGAT GTGGCTTTGA GTGTCCAAAG CCAACCCAGG 
ATGAAGGATA TTTCCCTGGC TGTCCGCCAC CACATGAGTC GAGCACCAAG CAGCACCTTT 
CATCCCCAGG ACTTCAGTGA CCTCATTAGT TTCATTTTGT ATGGGACCTT GCACCGTGGC 
GGTAAGGATC CCTGGCTGGA GCGATTGTAT TACAGCTGTC AGCGTTTTGA GAAGCTGGAT 
GGCCGAGGCT GCGCTGACAG TGGTAAGCCA GGTGTTGTAC CACAAGCCCT GCTGAAAAAT 
GAGGCTGTGC TGTGGCAGTG GGCAGCGTGG GAGGCTGCAC AATTCACTGT TCTGTCCAAA 
CTCCGAACGC CACTGGGCCG TGCTCCAGAC ACCTTTCAAA CCATTGAAGG TATGATCCGT 
AGCCTGGCTG CACATGCTGT CAACCCCGAA CAGGACGTCG GAGCGTGGGC TGGATCTGGG 
GGTGATGGGG ATGGTCACCA CTCCAATCAG CTCCGTCTTG CTCTGTTGCT TCAGTACCTG 
GAAAACCTGG AGAAATTAAT GTACAATGCA TATGAAGGAT GTGCCAATGC TCTAACTTCT 
CCTCCAAAGA GTATCAAAAC TTTCTTTTAC ACAAACCGTC AGACATGCCA AGATTGGCTA 
ACAAGGATCC GTCTTGCTCT GATGAGAGTT GGTCTTCTTT CTGGCAAACC AGCAGTCACC 
ATTCGCCATG GATTTGACTT GCTAACAGAG TTTAAGAACA GTAGTGCTCA GGGTCCAGAG 
GTGGAGGTCC TTCTGACATT GTTGGCAGAA GCTCTCTGTG AGCTAAATTG TCCTGAGGCC 
ATACAGGGTT TGGCAGCATG GAGCCTTTTG ACCAGTGGTA GAAGTCTTGG CTGGCTGTCA 
TCAGTCGCCC TACAGGCTGA GGGAAGGTTT GAGAAGGCAG CTCTTGAATA TCAAGATCAC 
CTCTCTGCAG TTACAGGGAT GGACTGTAGC ATCAAAAGCA GTGACTGCTT TTTGCCTAAA 
TTCTCCAACA GCACAAGTAG CCCCAAACAT GTGAACAATG GTGACAATAA GAAGACGGTG 
CTCCTCAAGG GAAATGAGTG TTCCTCTGAG GTCATCAACT TTCTGGTCAA CAAAGCTAGT 
CAGTGTTATG TGGCGCTCTG TGACTGGGCA TCAGTTAAAG AGTGGCAGAC CACTGTCCAT 
ACCCTAAAAC AAAACTCCAG CATTTCAGGA AGCATCAATT TGAGAACAGA TTTAAACTAC 
ATCCAGGCCC TGAGTCGCTT TGAGGAAGGT GATCTTGCTG AGTGTGCAAC TCAGCTTGAG 
CTGCTGCCTG GAGAGGACTA TAGTTTGCTC TCTTACAGCA AAGACAAACT GGACCTTAAG 
AAACTTCTGC CTTCCATGAG TCCGGATCCA ATTGAACTGC AAAGAGCCAT TGAAGTGCAG 
CTCCTTCGCA GTGCTGTTAG TTCCACGCCC AAAGCCACAC ACCAGGAGCC AAAAAGTACC 
CCAAGTCCCG ACATGTTGCT ACGATGCCTG AAGCAGACTG GAAGACTCTG TTTGGGTCCT 
CTCCGTTTGT CTACCCTCAA TGTGGCTGAC AACTTGCCCA CCCTGCCAAC CCTGCAGCTA 
CATTGCAGTT CCATGCTGGA GAACACACTC ATAGGACAGG AGGAATGTGT GATTCCTCTA 
TCCAGTGAGG CTTTAAGGTC TTGTAAACAA CAGGATGTCC AGCCTTTTCT TCATGCTTTC 
AGATACACTG TCTTCCAAAG ACAGCACCTT AATAAGGTTA AAGGCTACTC TTCATCGACA 
GATGGCCATC TAACGGAGCT TTGCTTGACG GCTGTCAAAT TTGCCAGGAA AACAGGAAAC 
ATTAGCCTAG CTTCCCGTTT GATTAGGCAG TGTGGTGATA GAGCCCATGA GGAGGAGGAG 
AAGGGTGATT TGAGCCTCAC ATTCAGGCGA CTCAATCTGG AGGGCACTCT TGGCGAGAAA 
TGGGGACCAG AGCTACAGAT AGAAAAAGCT AAAGTTCTTA GAGCTGCAGG CCAGTCCATG 
GCAGCCATGG AGATGCTAAG TGGAGCTGCA ATGTCATACT GCCACATGGG GAAGAATGAA 
GGGGCTGCCT GCCGCTCACT GCTCACACTT TGCAAATGGT TGCTGGCTGA CTGGAAAGAC 
ATGAGTTCTC AACTTAAACA GGTGATAAAG AAGTCAGGTG TAGTGAACTC CAGTGCCGGC 
AATATAACAT CCCTCACCAG AAACATTTCT TCACTGCTGG AACTTTCCCT CAAAGAGCAG 
GGTATTCCCC ACATCACCGC TGAGACCTCA GTAAATGTGG GTGTTGGGGA GCCAGACTTT 
GTTTTGGGCC AGCTCTACCA GCTGTCTACA TACCTGGCTC CAGAGATGGC AAAGTCCTGG 
GCAGCTCTGG CTAGTTGGGC TTATCGCTGG GGAAGGAAGG TGGTGGATAA TACCAGCCAT 
GGTGAAGGTT TGCCTCTTCT ACCTGAAGAG AAAAGAGAGA TAGAAGAGCT GCTTCCATCA 
ACTACCAATG ACGAAGATAA AGAGAAAATT TTCAGCATTC TCGGCCAGGC CATGTGTAGA 
CCTACTGGTA TACAGGATGA AGACATTGCT CTCCAGCAGG ATGATGATGA GGATGACATG 
GTGGATGTGA TCTGGAGACA ACTCACTGGC GCATGTCCCT GGCTTGCAAA TATGGGCCAG 
GGTGTCTCAG ATGGTCTGAT TCAGGTTTGG AGACATGTGG TGGATCGCAT CTTTGGCCTC 
TACAGGGTCT CATGCCAGGC CTATTTTACT TTTTTGAAGC TCAATGTTGG ACAGGTCCCT 
TTAGATGAAG ATGATCCAAA AGCCTTGCTG TCATTTCAAA ACAACAAACA GAGCAATGAT 
GATGTGATTG TCATGGCAAC CCTGCGGCTC CTTCGATTGC TGGTGAAGCA TGCTGGTGAA 
TTGAGAGAAG TACTGGAGTT GGGTTTAGCC TCGACCCCTA CAGGACCCTG GAGAGGGATC 
ATCCCCCAGC TTTTTTCCCG TCTCAATCAT CCAGAGGCAT ATATCCGGCA AAGCATATGC 
AGTTTGCTGT GTCGAGTGGC CCAAGACTCC CCGCATCTCA TACTCTACCC TGCCATTGTG 
GGCTCACTAT CCTTTGGAAC AGAAGCCCAG AATGCAGGAA ATCAACTACC TTCAGCACTT 
CCCACGTTTT TGGGAAGTAT GCAGGGAGAG GGCATTGTAG GAGAGGAGCA GGCTGATGGC 
AACGGACCAG ATGGAGCGCC AAAAGCAGAA CTAGCAAACT TGTACTCCAG TCAAGACCAA 
GCCATGATGC AGGATTGTTA CAGCAAGATA GTGGAAAAAC TGTCCATTGC AAATCCTGCC 
ATGGTGCTTC AGGTCCAACA GCTGGTGGGT GAACTACGCA GAGTTACCTT ACTCTGGGAT 
GAATTGTGGC TCGGTGTGTT GCAACAGCAA CATATGCATG TCCTTCGTAG GATCCAGCAG 
CTCGAGGATG AGGTTAAGAG GGTTCAGAAT AACGCCACGC TGCGCAGGGA TGAAAAGATT 
GCTATCATGC GGGAAAAACA CTCTGCTTTG ATGCGTCCTG TGGTTTTTGC TCTGGATCAC 
GTTTGCAGTA TCACTGCAGC CCCTGCAGAG ACACCACATG AAGTGTGGTT CCAGCAGACT 
TACGGGAGTG ATATCATATT TGCCCTTGAA CGACTGAGAA ACCCTAATAA CCCCGCCAAC 
CCTGCCAGCA GCTGGCTTCC CTTTAAACAG ATCATGTTGA GCCTGCAGCA GCGTGCCCAA 
AAGCGAGCTA GCTACCTGCT TCACCTGGAG GAGATCAGTC CTCATCTTGT GTCAATGACG 
AAGACAGAAA TGGCCTTGCC TGGTGAGGTG TCAGCATCAG ACGCTATCAC CATACAAAGC 
ATAGGCAATA CCATTACCAT TCTACCCACT AAGACCAAGC CCAAAAAGCT GTTCTTCATG 
GGCTCAGATG GACGACATTA CCCATACCTC TTTAAAGGTC TGGAGGATTT ACATTTGGAT 
GAGCGCATCA TGCAGTTTCT GTCCATTGTC AATACCATGT TCACCAAGAT TAACCAGCAA 
GAGCAGCCTC AATTCCATGC CTGCCACTAC TCTGTCACCC CGCTTGGAAC CAGGTCAGGG 
CTTATTCAGT GGGTGGATGG AGCCACACCA CTGTTTGGCC TCTACAAACG CTGGCAGCAA 
AGGGAGGCTG TGCTCCAGAC CCAGAAGGGC CAAGATTCAT TGCAGCAACA GCTGCCAATC 
CCTCCAGTAC CTCGCCCAAG TGAACTGTAC TACAGCCGCA TTGGGCCAGC TCTGAAGGCA 
GTGGGGCTCA GCCTGGACGT GACGCGACGA GATTGGCCTC TAAGTGTCAT GAAAGAGGTG 
CTAAAAGAGT TGATGGAGGC TACACCTTCC AACTTGCTTG CTAAAGAGCT GTGGTGTTCC 
TGCACCACGC CAAGTGAGTG GTGGAGAGTA ACTCAGTCTT ATGCCAGGTC CACTGCTGTC 
ATGTCGATGG TTGGCTATAT CATTGGCCTT GGTGACCGTC ATCTAGACAA TGTGTTGATT 
GACATGACCA CAGGAGAGGT GGTGCACATT GACTACAATG TATGCTTTGA GAAAGGGAAG 
AGTCTACGAG TGCCAGAGAA AGTCCCATTC AGGATGACCC ACAACATTGA GACTGCTCTG 
GGTGTCACCG GTGTGGAGGG CATCTTCAGG CTGTCCTGTG AACAGGTTCT TCAGATCATG 
CGTCGTGGCA GAGAGACCCT GTTGACCCTG CTGGAGGCCT TTGTCTACGA TCCTCTTGTG 
GACTGGACTG CTGGTGGGGA AGTGGGTTTT GCTGGCGCTG TTTATGGAGG AGGAGGCCAA 
CAAGCTGAAA ACAAGCAGAG CAAAAGGGAG ATGGAAAGGG ACATTACACG CTCTCTTTTC 
TCATCCAGGT TAGCTGAGAT TAAGGTGAAT TGGTTTAAAA ATCGTGATGA AATGCTGGCA 
GTGTTGCCAC AAGTGGAAGA GGCTGTGGAA GAGTACCTGG TTCTGCAGGA GCTGCTCTGC 
CAGGGTGGGA AACTGCAGGA CAAACTTCAA GAAGAGATAA CCTTCCTAGA AGGAGCAGAG 
AACCATCCAG ACCATCTTAT TCTTACCCTG GAGCAAAGGT ACTCTGAACA CACTCGGCTT 
CAGTCACAGC AGCGAACGAT GCAGGAGGCC ATTCAAAGCA AACTCACTGA CCTTGACCAG 
TGGATTTCCC AGTACCAGGC AGCTTTCTCC AACCTGGAAG CTGCCCAGCT GGCCACTTTG 
CTTCAAGACA TCAGTTGCCC CATAGATTTG GGTCCTCCCA GCTATGTACC TGCTACAGCC 
TTCCTACAGA ATGCAGGCCA GGCCCATTTG ATCACCCAGT GTGAGAACTT GGAAGCAGAA 
TTGAGCACTT TGCTGCAGCA GCGACGTGGG GCCCTTCGTG GTTGCCTAGA GCAGTTGCAC 
AGCTACGCAA CAGTAGCATT ACTGTACCCA AGAGGTACTT TGCGGCTCCA TCGAGTCCAG 
CAGTGGAAGG CCTGGTTAGA GGAATTGTTG ACAGACATGA CGGTGGAGCA GTGCCAGCAT 
ATCTACCGAC AATATGAGAT ACTCTTTGCA CCTCAGCCTT CTGCAGCTAT CTGTCAGTTT 
TTGTCAAGTG TGGAGTTGAC TTTGCAACAT CAGATGGCAG AAACCAATGA TCGTGTGATG 
CATCAGGCAG AGCGGCTCAA GGCCGAGGGA ACGACTGTGG CAGTGTGCGA AGATCAAGTA 
CAGGAGATTG ACCACTGTAT CACAGGTTTT CTACATGAGA ACAGAGAGTA TGGTTCTCTG 
GGATTGGCAG GAATCATCAC CTCTCTCCTG TGTACACTGT GCAGAAGAAA TCTGGTTATG 
GAAACTGCTG CAGCCAATGC TGGAGAACAG CTTATTGACT TAAAGTCTCG AGATGGGACA 
TGGTTCCTGG AGGAGCTCTG CAGTATGTTT GGAAATGTCA GTGGCCTGAC AACACTTCTA 
CAACGCTGCC AACTTGTACA ACATGATCTG GAGCTGCCTG CCCTCTCAGA CACCATCCAA 
GCTGTTTATC TGGCCAATGT TGTATATACC TGCTTACAAG AACTAAACAC CAATTTTCGA 
CAGATCATCT TTCCAGAAGC TTTGTGTTGT ATGATAAAAG GAGAGCCCAC TCTCGAGTCC 
ATGTTGGTGG AGATAGAGCA GCTGGTGGAG CAGAGCTCTG ATGGTCTTGG ACTGCAGGGA 
TTGGTTGACA GTCTGCAATC TACTATGCGG TTCGACCAAG ATGGACACAA CCATTTGCTT 
CACATTACAA GTTTACTGCG TGCTCAGTAC TCTGGGCTGA TCCAGCCTCG GAACACAGAA 
GGACCAGGTC ACGACACACC AAAGATGTCC CCAGGACAAA TGTTGTTGGT GGCGTTTGAT 
GGAATGTTCA CCCAGCTAGA GAATGCATTT ATGCAGCTCA CAGAGAAGCT GAGTTGTCTC 
GAGGTGCCTT CAGCCTGGCA AAAAATAGAT TCTTTGAGGG AAGCTCGGAC CGCACAGCTC 
TATTTCTTTG AGAATCGGAG TCAGAGACAA CTCATGGAAG AGGTATTCTT CCTCAAAAGA 
CTGCAGACCA TTAGGGATTT CTTCAAGCTT TGTGCCACAT TTGCCCAAAC TATCTCTGGT 
ACCTGTCCTT CAACTGAGGA CCTCTTAACA AATGGGCCAG TAGGATCGGG GAAGTCATTC 
TACCAGCTGC CCACTGCATC AGACTTGGGT GTGCCAGTGG TCAGTGAAGA AAAGATGACC 
CATCCGATTA AGGCCTTCAC AGCTGAGTTT GTCCGACAGA TGCTCATGGG TCTGCCCAGC 
CAAACACTAA GCCTAGTTCT GTGCAGCGCC CTTTCTTCTC TTGGGCTGGA TATTGTTTCC 
CTGGTGGAGG CCAAAGACTT TGGTGTTGAG AGAAAGGTTT CTCTGGATGA ACTTTGTAAG 
ACGGCAGTGG AGCAGAGCCT AGCTTCAGGC CGGGTTTCCC AGCTGTTGTT GAACAGAGCC 
ACTGTATTGG CCTCCTCCTA TGATACAGCT TGGAAGAAAC TAGACCTTCT CAGAAGACTG 
GATGCCCACC TGGAGGCTGC CAAGGCCAGT TTGCAGCGAA GCCAGCTGCA TATCGCCATG 
TTTCAGTGGC ATCATGAGGA TATTCTGGGC CCCAGAAATC AGCCTCTAGG TGTAAGCATT 
CCACCTCGTA CCATCATTCT TAGCAACATG AAGAAAAAAC TTTACAAGCT GAGTCAGGAT 
GAGGGAGCCA TAGTTGCTGT TCAGGAGAAG CTGGCGTCAC TGGAGGCTAG CATTGAGCAA 
CGACTAAAGT GGGCAGGTGG AGCCAATCCA GCACTGGCCC CAGTACTGCA AGACTTTGAG 
ATGACCATCA CTGAGAGACG TGCAATTGTG GCCAGAGAAA ACCAGCGCAC CAGCCAGGTC 
ACTTTTCTGT GCTCAACTAT CCTTAATTTT GAAGGCCTTC GTACCCGAAC ACCTGAGGCC 
CTGAATATGG ATGCAGCACT TTTTGAGTTG TTAAAACGTT GCCAGCAAAC CTGCTCCTTT 
GCTGCCCAGT TTAGCAGCAG TGTGTCTCCA CTTGAGCTTC AGCTGTTGCA AAGGCTGAGT 
CCAGTGCTTG ACTTGCCTAT TGGCAGCCCC GACTGGCTGA GCTGTGCACA AAAGCAACTG 
GAGGAGCAGC TTAATGTTGA GCAAAGCACT CGGGAGGAGC GGGAACAGCA GCTGGAGGTC 
TGTGCAGAGA CTCTGCAAGT TCTGGTCGAT TCTGTAAAGA CCATCCTGTC CCATCACAAC 
CGCCAGCTGG CAGATGTCAA GCACCTATTG AGAGCCATGG CAAAGGACGA GGAGGCAGCT 
TTGGCAGATG GTGAAGAGGT GATATATGAG GGTAGCGTGC GTCAGTTTCT GCTGGAGTAC 
AAAGCATGGC AAGACAACAT CCAGCTGGTT TTATTCACTG TGGTCCAGGC TAGTGGACAG 
CCTCGCAGTC AGGAGCAGCT TGAGCTGCTG GAAGACATTC CTGACACGCT GAAGGAACTG 
TACTCTCAAA GTGAGAGTGT GTATAATGGC TTAGTTGGCT TCGCTTCCCC ACTCGTGACT 
GACCGTGAAA GTGAAAGCAC CAGTCCAGTG TCTGCAATAC AGACGAGCTT TGCAGCAGCT 
GTCAGGTGCA CTGGGTTAAA GACCCAGCCA GACTCGGTCT CCCAGAATGT TCGCAAGGCT 
CTGCCGCGGA ATCTCGGAAC CCCTACTGAT ACCCCGCCCA GTGCTCCGTT GGTGAACAAC 
AAGTGTCTCA ATGCCAGCCC AAAGAGAGCC ATACGTGACC CCAAAACTGG ACGAGCTGTT 
CAGGAGAGAA ACTCCTATGC AGTCAGTGTG TGGAAGCGAG TGAAAGCCAA ACTTGAGGGA 
AGAGATATTG ATCCGAACAG AAGGATGAGT ATCACAGAGC AGGTGGACTT TGTCATCAAA 
GAGGCCACAA ATCTGGACAA CCTTGCGCAG CTATACGAAG GCTGGACTGC CTGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604331.1
CDS	142..11061
Translation	MSRKSSGSRLSGAHKLQPHWNDWQPRTDSASLSQEGELDSSILYDILPSSCPDPPVVSSNAFHAADTHQEEGGYEGNKVICINAFGWKSVGQELRPNDLTTDLPRFQHGNRALASKDMKKSQDRGMGHSEESRLANLLRRVSREDDRINILFFFPPLSTLVKQLDTILSTLNDILNESSKLLWELRQDAAICLGLLCTVLSYEAESIFKWLFLKLTSSNRDEVKLLYLMATYHALEAAGERKAFSHVMQLVMTNLQSILENVDTPELLCQSVKCILQVAHCYPHVFSINFRDTVDILVGWHIDHTQKQSVTQQVSGWLQSLEQFWVADLTFSTTLLGQFLEDMEAYAEDLNHVVLGEILDEDIPPPSVSLPKLAALLRVFSTVVRSIGERFSPLRGPPITEVYVTDVLNRVLACVSTAKQVQFSEEVLTAGNECVGVLLASVEPSGQLMEAVLVYGLDQLDCCQACSSDYNLSVLIILTMIVDQINTKLPASFVEKLLAPESKLMQLRFHRDKEVISAVHGVYQALLSLKNIPTLEAAYKMVLGEIACALSSLTLVLKANGMAPAPNGCLYSNIQHPAFTSLTLPLENAEFTLIFNLSTLTTIGNTKNSLIGMWALSPTVFTLLSQNLLLVHSDLAVYYPTIQYAVLYTLYSHCTRHDHFISSSLSSSPSLFDGAVISTVTTATKKHFSTLLGLLGGLLLKEHLYIETRRLLLTWAQEISVLMKKSDTYSPLFSLTTFNKFCRGLLANALNEDQSICLQTCHCIMVLSSSLSTELLQSCVDVCRVQLVNSAVRVRQAYGKLLKTIPLDVALSVQSQPRMKDISLAVRHHMSRAPSSTFHPQDFSDLISFILYGTLHRGGKDPWLERLYYSCQRFEKLDGRGCADSGKPGVVPQALLKNEAVLWQWAAWEAAQFTVLSKLRTPLGRAPDTFQTIEGMIRSLAAHAVNPEQDVGAWAGSGGDGDGHHSNQLRLALLLQYLENLEKLMYNAYEGCANALTSPPKSIKTFFYTNRQTCQDWLTRIRLALMRVGLLSGKPAVTIRHGFDLLTEFKNSSAQGPEVEVLLTLLAEALCELNCPEAIQGLAAWSLLTSGRSLGWLSSVALQAEGRFEKAALEYQDHLSAVTGMDCSIKSSDCFLPKFSNSTSSPKHVNNGDNKKTVLLKGNECSSEVINFLVNKASQCYVALCDWASVKEWQTTVHTLKQNSSISGSINLRTDLNYIQALSRFEEGDLAECATQLELLPGEDYSLLSYSKDKLDLKKLLPSMSPDPIELQRAIEVQLLRSAVSSTPKATHQEPKSTPSPDMLLRCLKQTGRLCLGPLRLSTLNVADNLPTLPTLQLHCSSMLENTLIGQEECVIPLSSEALRSCKQQDVQPFLHAFRYTVFQRQHLNKVKGYSSSTDGHLTELCLTAVKFARKTGNISLASRLIRQCGDRAHEEEEKGDLSLTFRRLNLEGTLGEKWGPELQIEKAKVLRAAGQSMAAMEMLSGAAMSYCHMGKNEGAACRSLLTLCKWLLADWKDMSSQLKQVIKKSGVVNSSAGNITSLTRNISSLLELSLKEQGIPHITAETSVNVGVGEPDFVLGQLYQLSTYLAPEMAKSWAALASWAYRWGRKVVDNTSHGEGLPLLPEEKREIEELLPSTTNDEDKEKIFSILGQAMCRPTGIQDEDIALQQDDDEDDMVDVIWRQLTGACPWLANMGQGVSDGLIQVWRHVVDRIFGLYRVSCQAYFTFLKLNVGQVPLDEDDPKALLSFQNNKQSNDDVIVMATLRLLRLLVKHAGELREVLELGLASTPTGPWRGIIPQLFSRLNHPEAYIRQSICSLLCRVAQDSPHLILYPAIVGSLSFGTEAQNAGNQLPSALPTFLGSMQGEGIVGEEQADGNGPDGAPKAELANLYSSQDQAMMQDCYSKIVEKLSIANPAMVLQVQQLVGELRRVTLLWDELWLGVLQQQHMHVLRRIQQLEDEVKRVQNNATLRRDEKIAIMREKHSALMRPVVFALDHVCSITAAPAETPHEVWFQQTYGSDIIFALERLRNPNNPANPASSWLPFKQIMLSLQQRAQKRASYLLHLEEISPHLVSMTKTEMALPGEVSASDAITIQSIGNTITILPTKTKPKKLFFMGSDGRHYPYLFKGLEDLHLDERIMQFLSIVNTMFTKINQQEQPQFHACHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAVLQTQKGQDSLQQQLPIPPVPRPSELYYSRIGPALKAVGLSLDVTRRDWPLSVMKEVLKELMEATPSNLLAKELWCSCTTPSEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTHNIETALGVTGVEGIFRLSCEQVLQIMRRGRETLLTLLEAFVYDPLVDWTAGGEVGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRLAEIKVNWFKNRDEMLAVLPQVEEAVEEYLVLQELLCQGGKLQDKLQEEITFLEGAENHPDHLILTLEQRYSEHTRLQSQQRTMQEAIQSKLTDLDQWISQYQAAFSNLEAAQLATLLQDISCPIDLGPPSYVPATAFLQNAGQAHLITQCENLEAELSTLLQQRRGALRGCLEQLHSYATVALLYPRGTLRLHRVQQWKAWLEELLTDMTVEQCQHIYRQYEILFAPQPSAAICQFLSSVELTLQHQMAETNDRVMHQAERLKAEGTTVAVCEDQVQEIDHCITGFLHENREYGSLGLAGIITSLLCTLCRRNLVMETAAANAGEQLIDLKSRDGTWFLEELCSMFGNVSGLTTLLQRCQLVQHDLELPALSDTIQAVYLANVVYTCLQELNTNFRQIIFPEALCCMIKGEPTLESMLVEIEQLVEQSSDGLGLQGLVDSLQSTMRFDQDGHNHLLHITSLLRAQYSGLIQPRNTEGPGHDTPKMSPGQMLLVAFDGMFTQLENAFMQLTEKLSCLEVPSAWQKIDSLREARTAQLYFFENRSQRQLMEEVFFLKRLQTIRDFFKLCATFAQTISGTCPSTEDLLTNGPVGSGKSFYQLPTASDLGVPVVSEEKMTHPIKAFTAEFVRQMLMGLPSQTLSLVLCSALSSLGLDIVSLVEAKDFGVERKVSLDELCKTAVEQSLASGRVSQLLLNRATVLASSYDTAWKKLDLLRRLDAHLEAAKASLQRSQLHIAMFQWHHEDILGPRNQPLGVSIPPRTIILSNMKKKLYKLSQDEGAIVAVQEKLASLEASIEQRLKWAGGANPALAPVLQDFEMTITERRAIVARENQRTSQVTFLCSTILNFEGLRTRTPEALNMDAALFELLKRCQQTCSFAAQFSSSVSPLELQLLQRLSPVLDLPIGSPDWLSCAQKQLEEQLNVEQSTREEREQQLEVCAETLQVLVDSVKTILSHHNRQLADVKHLLRAMAKDEEAALADGEEVIYEGSVRQFLLEYKAWQDNIQLVLFTVVQASGQPRSQEQLELLEDIPDTLKELYSQSESVYNGLVGFASPLVTDRESESTSPVSAIQTSFAAAVRCTGLKTQPDSVSQNVRKALPRNLGTPTDTPPSAPLVNNKCLNASPKRAIRDPKTGRAVQERNSYAVSVWKRVKAKLEGRDIDPNRRMSITEQVDFVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_011606029.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes SMG1 phosphatidylinositol 3-kinase-related kinase (smg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606029.1

ORF Insert Sequence:

ATGAGCCGTA AATCTTCGGG GTCTCGGCTC AGCGGCGCCC ACAAACTGCA GCCACACTGG 
AATGACTGGC AGCCCAGAAC TGACAGTGCG TCACTCAGCC AGGAGGGAGA GCTCGATTCA 
TCCATATTGT ATGATATATT GCCCTCTAGC TGTCCCGATC CTCCAGTGGT GTCTAGCAAT 
GCCTTTCATG CTGCAGATAC ACACCAGGAA GAGGGTGGAT ATGAAGGCAA CAAAGTTATC 
TGTATTAATG CGTTTGGTTG GAAGTCGGTG GGGCAAGAGC TGAGGCCAAA TGACTTGACC 
ACAGATTTGC CTCGTTTTCA GCATGGAAAT CGTGCTTTAG CTTCAAAGGA CATGAAAAAA 
TCGCAGGACA GAGGAATGGG TCATTCAGAG GAGTCTCGGC TGGCCAACCT CCTGCGTCGA 
GTTTCTAGGG AGGATGATCG TTAAATAAAT ATTCTTTTTT TTTTTCCTCC CCTCTCTTAG 
ACACTGGTTA AACAATTGGA CACAATCCTC AGCACCCTAA ATGATATTTT AAATGAGAGT 
AGTAAGCTAC TCTGGGAGCT GCGTCAGGAT GCAGCCATTT GCCTTGGTCT ACTCTGCACG 
GTTCTCAGCT ATGAGGCTGA GAGCATCTTC AAGTGGCTAT TTCTAAAATT AACATCGAGC 
AATCGCGATG AAGTGAAGCT GTTGTACCTT ATGGCAACAT ATCACGCATT GGAGGCTGCT 
GGAGAGAGGA AGGCCTTTTC TCACGTCATG CAGCTGGTGA TGACCAATTT GCAGTCCATC 
CTGGAGAATG TTGACACACC AGAGCTGCTT TGTCAGAGTG TGAAGTGTAT TCTTCAGGTG 
GCGCACTGTT ACCCACATGT GTTCAGCATC AACTTTAGAG ATACAGTTGA TATATTGGTG 
GGCTGGCACA TAGACCACAC TCAGAAGCAG TCGGTCACAC AGCAAGTTTC AGGTTGGCTT 
CAGAGTCTGG AACAGTTCTG GGTGGCGGAC CTGACATTCT CCACCACACT ACTGGGACAG 
TTCCTGGAGG ATATGGAAGC ATATGCAGAG GATCTGAACC ATGTTGTTTT AGGGGAGATT 
CTCGATGAAG ATATTCCACC TCCCTCGGTG TCACTGCCAA AGTTAGCAGC ACTGTTGAGA 
GTTTTCAGCA CAGTGGTCCG CAGCATTGGA GAGCGATTTA GTCCCCTAAG AGGACCACCC 
ATTACGGAGG TCTACGTCAC AGATGTTCTG AACCGTGTTC TGGCCTGTGT GTCGACGGCA 
AAGCAGGTTC AGTTTTCTGA AGAAGTTCTC ACTGCTGGAA ACGAGTGTGT GGGTGTGTTG 
CTAGCTAGTG TGGAACCCAG TGGTCAGTTG ATGGAGGCTG TCTTGGTCTA TGGGCTGGAC 
CAGTTGGACT GCTGCCAGGC CTGCAGCTCA GACTACAACC TTTCAGTGCT CATCATCCTC 
ACTATGATAG TGGACCAAAT CAACACAAAG CTCCCAGCAT CATTTGTTGA AAAACTGTTG 
GCTCCAGAAT CCAAGTTGAT GCAGTTGCGG TTTCACAGAG ATAAAGAGGT GATTTCAGCT 
GTTCATGGTG TGTATCAGGC ATTGTTAAGC CTGAAGAACA TTCCTACACT TGAAGCAGCA 
TATAAAATGG TATTGGGTGA GATAGCATGT GCCCTCTCCA GCCTAACTTT GGTGCTGAAA 
GCCAATGGTA TGGCTCCTGC CCCCAATGGC TGCCTCTACT CAAATATCCA ACATCCTGCC 
TTTACTTCTC TCACCCTACC TTTGGAAAAT GCAGAGTTCA CACTGATCTT CAACCTCAGC 
ACCCTCACTA CCATTGGTAA CACCAAGAAT TCTCTGATTG GGATGTGGGC CTTGTCTCCT 
ACAGTCTTCA CACTGCTCAG TCAAAATCTT TTGTTGGTCC ACTCTGACTT AGCAGTGTAC 
TACCCCACCA TCCAGTATGC CGTACTTTAC ACACTGTACT CCCACTGCAC AAGACATGAT 
CATTTCATCT CATCCAGCTT ATCATCTTCT CCATCACTAT TTGATGGTGC TGTTATCAGC 
ACTGTGACCA CAGCAACAAA GAAACACTTC AGCACACTCT TAGGCCTTCT TGGAGGTCTC 
CTGTTGAAAG AACATCTCTA CATTGAGACC AGGAGACTGC TGTTGACCTG GGCTCAGGAA 
ATCTCGGTGC TGATGAAAAA GTCAGACACT TATAGCCCTC TCTTCTCTTT GACCACTTTC 
AACAAGTTTT GCAGGGGTCT CCTTGCCAAT GCTCTAAATG AAGATCAGAG TATTTGTCTT 
CAGACCTGCC ACTGTATTAT GGTTCTGTCT TCATCTTTGT CCACTGAGCT GCTGCAGAGC 
TGTGTGGATG TGTGTAGAGT ACAGTTGGTC AACTCAGCAG TAAGAGTAAG GCAGGCCTAT 
GGTAAGCTGC TCAAGACCAT TCCACTGGAT GTGGCTTTGA GTGTCCAAAG CCAACCCAGG 
ATGAAGGATA TTTCCCTGGC TGTCCGCCAC CACATGAGTC GAGCACCAAG CAGCACCTTT 
CATCCCCAGG ACTTCAGTGA CCTCATTAGT TTCATTTTGT ATGGGACCTT GCACCGTGGC 
GGTAAGGATC CCTGGCTGGA GCGATTGTAT TACAGCTGTC AGCGTTTTGA GAAGCTGGAT 
GGCCGAGGCT GCGCTGACAG TGGTAAGCCA GGTGTTGTAC CACAAGCCCT GCTGAAAAAT 
GAGGCTGTGC TGTGGCAGTG GGCAGCGTGG GAGGCTGCAC AATTCACTGT TCTGTCCAAA 
CTCCGAACGC CACTGGGCCG TGCTCCAGAC ACCTTTCAAA CCATTGAAGG TATGATCCGT 
AGCCTGGCTG CACATGCTGT CAACCCCGAA CAGGACGTCG GAGCGTGGGC TGGATCTGGG 
GGTGATGGGG ATGGTCACCA CTCCAATCAG CTCCGTCTTG CTCTGTTGCT TCAGTACCTG 
GAAAACCTGG AGAAATTAAT GTACAATGCA TATGAAGGAT GTGCCAATGC TCTAACTTCT 
CCTCCAAAGA GTATCAAAAC TTTCTTTTAC ACAAACCGTC AGACATGCCA AGATTGGCTA 
ACAAGGATCC GTCTTGCTCT GATGAGAGTT GGTCTTCTTT CTGGCAAACC AGCAGTCACC 
ATTCGCCATG GATTTGACTT GCTAACAGAG TTTAAGAACA GTAGTGCTCA GGGTCCAGAG 
GTGGAGGTCC TTCTGACATT GTTGGCAGAA GCTCTCTGTG AGCTAAATTG TCCTGAGGCC 
ATACAGGGTT TGGCAGCATG GAGCCTTTTG ACCAGTGGTA GAAGTCTTGG CTGGCTGTCA 
TCAGTCGCCC TACAGGCTGA GGGAAGGTTT GAGAAGGCAG CTCTTGAATA TCAAGATCAC 
CTCTCTGCAG TTACAGGGAT GGACTGTAGC ATCAAAAGCA GTGACTGCTT TTTGCCTAAA 
TTCTCCAACA GCACAAGTAG CCCCAAACAT GTGAACAATG GTGACAATAA GAAGACGGTG 
CTCCTCAAGG GAAATGAGTG TTCCTCTGAG GTCATCAACT TTCTGGTCAA CAAAGCTAGT 
CAGTGTTATG TGGCGCTCTG TGACTGGGCA TCAGTTAAAG AGTGGCAGAC CACTGTCCAT 
ACCCTAAAAC AAAACTCCAG CATTTCAGGA AGCATCAATT TGAGAACAGA TTTAAACTAC 
ATCCAGGCCC TGAGTCGCTT TGAGGAAGGT GATCTTGCTG AGTGTGCAAC TCAGCTTGAG 
CTGCTGCCTG GAGAGGACTA TAGTTTGCTC TCTTACAGCA AAGACAAACT GGACCTTAAG 
AAACTTCTGC CTTCCATGAG TCCGGATCCA ATTGAACTGC AAAGAGCCAT TGAAGTGCAG 
CTCCTTCGCA GTGCTGTTAG TTCCACGCCC AAAGCCACAC ACCAGGAGCC AAAAAGTACC 
CCAAGTCCCG ACATGTTGCT ACGATGCCTG AAGCAGACTG GAAGACTCTG TTTGGGTCCT 
CTCCGTTTGT CTACCCTCAA TGTGGCTGAC AACTTGCCCA CCCTGCCAAC CCTGCAGCTA 
CATTGCAGTT CCATGCTGGA GAACACACTC ATAGGACAGG AGGAATGTGT GATTCCTCTA 
TCCAGTGAGG CTTTAAGGTC TTGTAAACAA CAGGATGTCC AGCCTTTTCT TCATGCTTTC 
AGATACACTG TCTTCCAAAG ACAGCACCTT AATAAGGTTA AAGGCTACTC TTCATCGACA 
GATGGCCATC TAACGGAGCT TTGCTTGACG GCTGTCAAAT TTGCCAGGAA AACAGGAAAC 
ATTAGCCTAG CTTCCCGTTT GATTAGGCAG TGTGGTGATA GAGCCCATGA GGAGGAGGAG 
AAGGGTGATT TGAGCCTCAC ATTCAGGCGA CTCAATCTGG AGGGCACTCT TGGCGAGAAA 
TGGGGACCAG AGCTACAGAT AGAAAAAGCT AAAGTTCTTA GAGCTGCAGG CCAGTCCATG 
GCAGCCATGG AGATGCTAAG TGGAGCTGCA ATGTCATACT GCCACATGGG GAAGAATGAA 
GGGGCTGCCT GCCGCTCACT GCTCACACTT TGCAAATGGT TGCTGGCTGA CTGGAAAGAC 
ATGAGTTCTC AACTTAAACA GGTGATAAAG AAGTCAGGTG TAGTGAACTC CAGTGCCGGC 
AATATAACAT CCCTCACCAG AAACATTTCT TCACTGCTGG AACTTTCCCT CAAAGAGCAG 
GGTATTCCCC ACATCACCGC TGAGACCTCA GTAAATGTGG GTGTTGGGGA GCCAGACTTT 
GTTTTGGGCC AGCTCTACCA GCTGTCTACA TACCTGGCTC CAGAGATGGC AAAGTCCTGG 
GCAGCTCTGG CTAGTTGGGC TTATCGCTGG GGAAGGAAGG TGGTGGATAA TACCAGCCAT 
GGTGAAGGTT TGCCTCTTCT ACCTGAAGAG AAAAGAGAGA TAGAAGAGCT GCTTCCATCA 
ACTACCAATG ACGAAGATAA AGAGAAAATT TTCAGCATTC TCGGCCAGGC CATGTGTAGA 
CCTACTGGTA TACAGGATGA AGACATTGCT CTCCAGCAGG ATGATGATGA GGATGACATG 
GTGGATGTGA TCTGGAGACA ACTCACTGGC GCATGTCCCT GGCTTGCAAA TATGGGCCAG 
GGTGTCTCAG ATGGTCTGAT TCAGGTTTGG AGACATGTGG TGGATCGCAT CTTTGGCCTC 
TACAGGGTCT CATGCCAGGC CTATTTTACT TTTTTGAAGC TCAATGTTGG ACAGGTCCCT 
TTAGATGAAG ATGATCCAAA AGCCTTGCTG TCATTTCAAA ACAACAAACA GAGCAATGAT 
GATGTGATTG TCATGGCAAC CCTGCGGCTC CTTCGATTGC TGGTGAAGCA TGCTGGTGAA 
TTGAGAGAAG TACTGGAGTT GGGTTTAGCC TCGACCCCTA CAGGACCCTG GAGAGGGATC 
ATCCCCCAGC TTTTTTCCCG TCTCAATCAT CCAGAGGCAT ATATCCGGCA AAGCATATGC 
AGTTTGCTGT GTCGAGTGGC CCAAGACTCC CCGCATCTCA TACTCTACCC TGCCATTGTG 
GGCTCACTAT CCTTTGGAAC AGAAGCCCAG AATGCAGGAA ATCAACTACC TTCAGCACTT 
CCCACGTTTT TGGGAAGTAT GCAGGGAGAG GGCATTGTAG GAGAGGAGCA GGCTGATGGC 
AACGGACCAG ATGGAGCGCC AAAAGCAGAA CTAGCAAACT TGTACTCCAG TCAAGACCAA 
GCCATGATGC AGGATTGTTA CAGCAAGATA GTGGAAAAAC TGTCCATTGC AAATCCTGCC 
ATGGTGCTTC AGGTCCAACA GCTGGTGGGT GAACTACGCA GAGTTACCTT ACTCTGGGAT 
GAATTGTGGC TCGGTGTGTT GCAACAGCAA CATATGCATG TCCTTCGTAG GATCCAGCAG 
CTCGAGGATG AGGTTAAGAG GGTTCAGAAT AACGCCACGC TGCGCAGGGA TGAAAAGATT 
GCTATCATGC GGGAAAAACA CTCTGCTTTG ATGCGTCCTG TGGTTTTTGC TCTGGATCAC 
GTTTGCAGTA TCACTGCAGC CCCTGCAGAG ACACCACATG AAGTGTGGTT CCAGCAGACT 
TACGGGAGTG ATATCATATT TGCCCTTGAA CGACTGAGAA ACCCTAATAA CCCCGCCAAC 
CCTGCCAGCA GCTGGCTTCC CTTTAAACAG ATCATGTTGA GCCTGCAGCA GCGTGCCCAA 
AAGCGAGCTA GCTACCTGCT TCACCTGGAG GAGATCAGTC CTCATCTTGT GTCAATGACG 
AAGACAGAAA TGGCCTTGCC TGGTGAGGTG TCAGCATCAG ACGCTATCAC CATACAAAGC 
ATAGGCAATA CCATTACCAT TCTACCCACT AAGACCAAGC CCAAAAAGCT GTTCTTCATG 
GGCTCAGATG GACGACATTA CCCATACCTC TTTAAAGGTC TGGAGGATTT ACATTTGGAT 
GAGCGCATCA TGCAGTTTCT GTCCATTGTC AATACCATGT TCACCAAGAT TAACCAGCAA 
GAGCAGCCTC AATTCCATGC CTGCCACTAC TCTGTCACCC CGCTTGGAAC CAGGTCAGGG 
CTTATTCAGT GGGTGGATGG AGCCACACCA CTGTTTGGCC TCTACAAACG CTGGCAGCAA 
AGGGAGGCTG TGCTCCAGAC CCAGAAGGGC CAAGATTCAT TGCAGCAACA GCTGCCAATC 
CCTCCAGTAC CTCGCCCAAG TGAACTGTAC TACAGCCGCA TTGGGCCAGC TCTGAAGGCA 
GTGGGGCTCA GCCTGGACGT GACGCGACGA GATTGGCCTC TAAGTGTCAT GAAAGAGGTG 
CTAAAAGAGT TGATGGAGGC TACACCTTCC AACTTGCTTG CTAAAGAGCT GTGGTGTTCC 
TGCACCACGC CAAGTGAGTG GTGGAGAGTA ACTCAGTCTT ATGCCAGGTC CACTGCTGTC 
ATGTCGATGG TTGGCTATAT CATTGGCCTT GGTGACCGTC ATCTAGACAA TGTGTTGATT 
GACATGACCA CAGGAGAGGT GGTGCACATT GACTACAATG TATGCTTTGA GAAAGGGAAG 
AGTCTACGAG TGCCAGAGAA AGTCCCATTC AGGATGACCC ACAACATTGA GACTGCTCTG 
GGTGTCACCG GTGTGGAGGG CATCTTCAGG CTGTCCTGTG AACAGGTTCT TCAGATCATG 
CGTCGTGGCA GAGAGACCCT GTTGACCCTG CTGGAGGCCT TTGTCTACGA TCCTCTTGTG 
GACTGGACTG CTGGTGGGGA AGTGGGTTTT GCTGGCGCTG TTTATGGAGG AGGAGGCCAA 
CAAGCTGAAA ACAAGCAGAG CAAAAGGGAG ATGGAAAGGG ACATTACACG CTCTCTTTTC 
TCATCCAGGT TAGCTGAGAT TAAGGTGAAT TGGTTTAAAA ATCGTGATGA AATGCTGGCA 
GTGTTGCCAC AAGTGGAAGA GGCTGTGGAA GAGTACCTGG TTCTGCAGGA GCTGCTCTGC 
CAGGGTGGGA AACTGCAGGA CAAACTTCAA GAAGAGATAA CCTTCCTAGA AGGAGCAGAG 
AACCATCCAG ACCATCTTAT TCTTACCCTG GAGCAAAGGT ACTCTGAACA CACTCGGCTT 
CAGTCACAGC AGCGAACGAT GCAGGAGGCC ATTCAAAGCA AACTCACTGA CCTTGACCAG 
TGGATTTCCC AGTACCAGGC AGCTTTCTCC AACCTGGAAG CTGCCCAGCT GGCCACTTTG 
CTTCAAGACA TCAGTTGCCC CATAGATTTG GGTCCTCCCA GCTATGTACC TGCTACAGCC 
TTCCTACAGA ATGCAGGCCA GGCCCATTTG ATCACCCAGT GTGAGAACTT GGAAGCAGAA 
TTGAGCACTT TGCTGCAGCA GCGACGTGGG GCCCTTCGTG GTTGCCTAGA GCAGTTGCAC 
AGCTACGCAA CAGTAGCATT ACTGTACCCA AGAGGTACTT TGCGGCTCCA TCGAGTCCAG 
CAGTGGAAGG CCTGGTTAGA GGAATTGTTG ACAGACATGA CGGTGGAGCA GTGCCAGCAT 
ATCTACCGAC AATATGAGAT ACTCTTTGCA CCTCAGCCTT CTGCAGCTAT CTGTCAGTTT 
TTGTCAAGTG TGGAGTTGAC TTTGCAACAT CAGATGGCAG AAACCAATGA TCGTGTGATG 
CATCAGGCAG AGCGGCTCAA GGCCGAGGGA ACGACTGTGG CAGTGTGCGA AGATCAAGTA 
CAGGAGATTG ACCACTGTAT CACAGGTTTT CTACATGAGA ACAGAGAGTA TGGTTCTCTG 
GGATTGGCAG GAATCATCAC CTCTCTCCTG TGTACACTGT GCAGAAGAAA TCTGGTTATG 
GAAACTGCTG CAGCCAATGC TGGAGAACAG CTTATTGACT TAAAGTCTCG AGATGGGACA 
TGGTTCCTGG AGGAGCTCTG CAGTATGTTT GGAAATGTCA GTGGCCTGAC AACACTTCTA 
CAACGCTGCC AACTTGTACA ACATGATCTG GAGCTGCCTG CCCTCTCAGA CACCATCCAA 
GCTGTTTATC TGGCCAATGT TGTATATACC TGCTTACAAG AACTAAACAC CAATTTTCGA 
CAGATCATCT TTCCAGAAGC TTTGTGTTGT ATGATAAAAG GAGAGCCCAC TCTCGAGTCC 
ATGTTGGTGG AGATAGAGCA GCTGGTGGAG CAGAGCTCTG ATGGTCTTGG ACTGCAGGGA 
TTGGTTGACA GTCTGCAATC TACTATGCGG TTCGACCAAG ATGGACACAA CCATTTGCTT 
CACATTACAA GTTTACTGCG TGCTCAGTAC TCTGGGCTGA TCCAGCCTCG GAACACAGAA 
GGACCAGGTC ACGACACACC AAAGATGTCC CCAGGACAAA TGTTGTTGGT GGCGTTTGAT 
GGAATGTTCA CCCAGCTAGA GAATGCATTT ATGCAGCTCA CAGAGAAGCT GAGTTGTCTC 
GAGGTGCCTT CAGCCTGGCA AAAAATAGAT TCTTTGAGGG AAGCTCGGAC CGCACAGCTC 
TATTTCTTTG AGAATCGGAG TCAGAGACAA CTCATGGAAG AGGTATTCTT CCTCAAAAGA 
CTGCAGACCA TTAGGGATTT CTTCAAGCTT TGTGCCACAT TTGCCCAAAC TATCTCTGGT 
ACCTGTCCTT CAACTGAGGA CCTCTTAACA AATGGGCCAG TAGGATCGGG GAAGTCATTC 
TACCAGCTGC CCACTGCATC AGACTTGGGT GTGCCAGTGG TCAGTGAAGA AAAGATGACC 
CATCCGATTA AGGCCTTCAC AGCTGAGTTT GTCCGACAGA TGCTCATGGG TCTGCCCAGC 
CAAACACTAA GCCTAGTTCT GTGCAGCGCC CTTTCTTCTC TTGGGCTGGA TATTGTTTCC 
CTGGTGGAGG CCAAAGACTT TGGTGTTGAG AGAAAGGTTT CTCTGGATGA ACTTTGTAAG 
ACGGCAGTGG AGCAGAGCCT AGCTTCAGGC CGGGTTTCCC AGCTGTTGTT GAACAGAGCC 
ACTGTATTGG CCTCCTCCTA TGATACAGCT TGGAAGAAAC TAGACCTTCT CAGAAGACTG 
GATGCCCACC TGGAGGCTGC CAAGGCCAGT TTGCAGCGAA GCCAGCTGCA TATCGCCATG 
TTTCAGTGGC ATCATGAGGA TATTCTGGGC CCCAGAAATC AGCCTCTAGG TGTAAGCATT 
CCACCTCGTA CCATCATTCT TAGCAACATG AAGAAAAAAC TTTACAAGCT GAGTCAGGAT 
GAGGGAGCCA TAGTTGCTGT TCAGGAGAAG CTGGCGTCAC TGGAGGCTAG CATTGAGCAA 
CGACTAAAGT GGGCAGGTGG AGCCAATCCA GCACTGGCCC CAGTACTGCA AGACTTTGAG 
ATGACCATCA CTGAGAGACG TGCAATTGTG GCCAGAGAAA ACCAGCGCAC CAGCCAGGTC 
ACTTTTCTGT GCTCAACTAT CCTTAATTTT GAAGGCCTTC GTACCCGAAC ACCTGAGGCC 
CTGAATATGG ATGCAGCACT TTTTGAGTTG TTAAAACGTT GCCAGCAAAC CTGCTCCTTT 
GCTGCCCAGT TTAGCAGCAG TGTGTCTCCA CTTGAGCTTC AGCTGTTGCA AAGGCTGAGT 
CCAGTGCTTG ACTTGCCTAT TGGCAGCCCC GACTGGCTGA GCTGTGCACA AAAGCAACTG 
GAGGAGCAGC TTAATGTTGA GCAAAGCACT CGGGAGGAGC GGGAACAGCA GCTGGAGGTC 
TGTGCAGAGA CTCTGCAAGT TCTGGTCGAT TCTGTAAAGA CCATCCTGTC CCATCACAAC 
CGCCAGCTGG CAGATGTCAA GCACCTATTG AGAGCCATGG CAAAGGACGA GGAGGCAGCT 
TTGGCAGATG GTGAAGAGGT GATATATGAG GGTAGCGTGC GTCAGTTTCT GCTGGAGTAC 
AAAGCATGGC AAGACAACAT CCAGCTGGTT TTATTCACTG TGGTCCAGGC TAGTGGACAG 
CCTCGCAGTC AGGAGCAGCT TGAGCTGCTG GAAGACATTC CTGACACGCT GAAGGAACTG 
TACTCTCAAA GTGAGAGTGT GTATAATGGC TTAGTTGGCT TCGCTTCCCC ACTCGTGACT 
GACCGTGAAA GTGAAAGCAC CAGTCCAGTG TCTGCAATAC AGACGAGCTT TGCAGCAGCT 
GTCAGGTGCA CTGGGTTAAA GACCCAGCCA GACTCGGTCT CCCAGAATGT TCGCAAGGCT 
CTGCCGCGGA ATCTCGGAAC CCCTACTGAT ACCCCGCCCA GTGCTCCGTT GGTGAACAAC 
AAGTGTCTCA ATGCCAGCCC AAAGAGAGCC ATACGTGACC CCAAAACTGG ACGAGCTGTT 
CAGGAGAGAA ACTCCTATGC AGTCAGTGTG TGGAAGCGAG TGAAAGCCAA ACTTGAGGGA 
AGAGATATTG ATCCGAACAG AAGGATGAGT ATCACAGAGC AGGTGGACTT TGTCATCAAA 
GAGGCCACAA ATCTGGACAA CCTTGCGCAG CTATACGAAG GCTGGACTGC CTGGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg1 ( XM_029839735.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029695595.1 Takifugu rubripes SMG1 nonsense mediated mRNA decay associated PI3K related kinase (smg1), transcript variant X1, mRNA.

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CloneID	OTo70534
Clone ID Related Accession (Same CDS sequence)	XM_029839735.1
Accession Version	XM_029839735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	serine/threonine-protein kinase SMG1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGTA AATCTTCGGG GTCTCGGCTC AGCGGCGCCC ACAAACTGCA GCCACACTGG 
AATGACTGGC AGCCCAGAAC TGACAGTGCG TCACTCAGCC AGGAGGGAGA GCTCGATTCA 
TCCATATTGT ATGATATATT GCCCTCTAGC TGTCCCGATC CTCCAGTGGT GTCTAGCAAT 
GCCTTTCATG CTGCAGATAC ACACCAGGAA GAGGGTGGAT ATGAAGGCAA CAAAGTTATC 
TGTATTAATG CGTTTGGTTG GAAGTCGGTG GGGCAAGAGC TGAGGCCAAA TGACTTGACC 
ACAGATTTGC CTCGTTTTCA GCATGGAAAT CGTGCTTTAG CTTCAAAGGA CATGAAAAAA 
TCGCAGGACA GAGGAATGGG TCATTCAGAG GAGTCTCGGC TGGCCAACCT CCTGCGTCGA 
GTTTCTAGGG AGGATGATCG TGACCGCAGA CTGGCAACTC TCAGACAACT GAAGGACTTC 
ATTAGCCACA GTGAGAGTAA AGTGACACTG GTTAAACAAT TGGACACAAT CCTCAGCACC 
CTAAATGATA TTTTAAATGA GAGTAGTAAG CTACTCTGGG AGCTGCGTCA GGATGCAGCC 
ATTTGCCTTG GTCTACTCTG CACGGTTCTC AGCTATGAGG CTGAGAGCAT CTTCAAGTGG 
CTATTTCTAA AATTAACATC GAGCAATCGC GATGAAGTGA AGCTGTTGTA CCTTATGGCA 
ACATATCACG CATTGGAGGC TGCTGGAGAG AGGAAGGCCT TTTCTCACGT CATGCAGCTG 
GTGATGACCA ATTTGCAGTC CATCCTGGAG AATGTTGACA CACCAGAGCT GCTTTGTCAG 
AGTGTGAAGT GTATTCTTCA GGTGGCGCAC TGTTACCCAC ATGTGTTCAG CATCAACTTT 
AGAGATACAG TTGATATATT GGTGGGCTGG CACATAGACC ACACTCAGAA GCAGTCGGTC 
ACACAGCAAG TTTCAGGTTG GCTTCAGAGT CTGGAACAGT TCTGGGTGGC GGACCTGACA 
TTCTCCACCA CACTACTGGG ACAGTTCCTG GAGGATATGG AAGCATATGC AGAGGATCTG 
AACCATGTTG TTTTAGGGGA GATTCTCGAT GAAGATATTC CACCTCCCTC GGTGTCACTG 
CCAAAGTTAG CAGCACTGTT GAGAGTTTTC AGCACAGTGG TCCGCAGCAT TGGAGAGCGA 
TTTAGTCCCC TAAGAGGACC ACCCATTACG GAGGTCTACG TCACAGATGT TCTGAACCGT 
GTTCTGGCCT GTGTGTCGAC GGCAAAGCAG GTTCAGTTTT CTGAAGAAGT TCTCACTGCT 
GGAAACGAGT GTGTGGGTGT GTTGCTAGCT AGTGTGGAAC CCAGTGGTCA GTTGATGGAG 
GCTGTCTTGG TCTATGGGCT GGACCAGTTG GACTGCTGCC AGGCCTGCAG CTCAGACTAC 
AACCTTTCAG TGCTCATCAT CCTCACTATG ATAGTGGACC AAATCAACAC AAAGCTCCCA 
GCATCATTTG TTGAAAAACT GTTGGCTCCA GAATCCAAGT TGATGCAGTT GCGGTTTCAC 
AGAGATAAAG AGGTGATTTC AGCTGTTCAT GGTGTGTATC AGGCATTGTT AAGCCTGAAG 
AACATTCCTA CACTTGAAGC AGCATATAAA ATGGTATTGG GTGAGATAGC ATGTGCCCTC 
TCCAGCCTAA CTTTGGTGCT GAAAGCCAAT GGTATGGCTC CTGCCCCCAA TGGCTGCCTC 
TACTCAAATA TCCAACATCC TGCCTTTACT TCTCTCACCC TACCTTTGGA AAATGCAGAG 
TTCACACTGA TCTTCAACCT CAGCACCCTC ACTACCATTG GTAACACCAA GAATTCTCTG 
ATTGGGATGT GGGCCTTGTC TCCTACAGTC TTCACACTGC TCAGTCAAAA TCTTTTGTTG 
GTCCACTCTG ACTTAGCAGT GTACTACCCC ACCATCCAGT ATGCCGTACT TTACACACTG 
TACTCCCACT GCACAAGACA TGATCATTTC ATCTCATCCA GCTTATCATC TTCTCCATCA 
CTATTTGATG GTGCTGTTAT CAGCACTGTG ACCACAGCAA CAAAGAAACA CTTCAGCACA 
CTCTTAGGCC TTCTTGGAGG TCTCCTGTTG AAAGAACATC TCTACATTGA GACCAGGAGA 
CTGCTGTTGA CCTGGGCTCA GGAAATCTCG GTGCTGATGA AAAAGTCAGA CACTTATAGC 
CCTCTCTTCT CTTTGACCAC TTTCAACAAG TTTTGCAGGG GTCTCCTTGC CAATGCTCTA 
AATGAAGATC AGAGTATTTG TCTTCAGACC TGCCACTGTA TTATGGTTCT GTCTTCATCT 
TTGTCCACTG AGCTGCTGCA GAGCTGTGTG GATGTGTGTA GAGTACAGTT GGTCAACTCA 
GCAGTAAGAG TAAGGCAGGC CTATGGTAAG CTGCTCAAGA CCATTCCACT GGATGTGGCT 
TTGAGTGTCC AAAGCCAACC CAGGATGAAG GATATTTCCC TGGCTGTCCG CCACCACATG 
AGTCGAGCAC CAAGCAGCAC CTTTCATCCC CAGGACTTCA GTGACCTCAT TAGTTTCATT 
TTGTATGGGA CCTTGCACCG TGGCGGTAAG GATCCCTGGC TGGAGCGATT GTATTACAGC 
TGTCAGCGTT TTGAGAAGCT GGATGGCCGA GGCTGCGCTG ACAGTGGTAA GCCAGGTGTT 
GTACCACAAG CCCTGCTGAA AAATGAGGCT GTGCTGTGGC AGTGGGCAGC GTGGGAGGCT 
GCACAATTCA CTGTTCTGTC CAAACTCCGA ACGCCACTGG GCCGTGCTCC AGACACCTTT 
CAAACCATTG AAGGTATGAT CCGTAGCCTG GCTGCACATG CTGTCAACCC CGAACAGGAC 
GTCGGAGCGT GGGCTGGATC TGGGGGTGAT GGGGATGGTC ACCACTCCAA TCAGCTCCGT 
CTTGCTCTGT TGCTTCAGTA CCTGGAAAAC CTGGAGAAAT TAATGTACAA TGCATATGAA 
GGATGTGCCA ATGCTCTAAC TTCTCCTCCA AAGAGTATCA AAACTTTCTT TTACACAAAC 
CGTCAGACAT GCCAAGATTG GCTAACAAGG ATCCGTCTTG CTCTGATGAG AGTTGGTCTT 
CTTTCTGGCA AACCAGCAGT CACCATTCGC CATGGATTTG ACTTGCTAAC AGAGTTTAAG 
AACAGTAGTG CTCAGGGTCC AGAGGTGGAG GTCCTTCTGA CATTGTTGGC AGAAGCTCTC 
TGTGAGCTAA ATTGTCCTGA GGCCATACAG GGTTTGGCAG CATGGAGCCT TTTGACCAGT 
GGTAGAAGTC TTGGCTGGCT GTCATCAGTC GCCCTACAGG CTGAGGGAAG GTTTGAGAAG 
GCAGCTCTTG AATATCAAGA TCACCTCTCT GCAGTTACAG GGATGGACTG TAGCATCAAA 
AGCAGTGACT GCTTTTTGCC TAAATTCTCC AACAGCACAA GTAGCCCCAA ACATGTGAAC 
AATGGTGACA ATAAGAAGAC GGTGCTCCTC AAGGGAAATG AGTGTTCCTC TGAGGTCATC 
AACTTTCTGG TCAACAAAGC TAGTCAGTGT TATGTGGCGC TCTGTGACTG GGCATCAGTT 
AAAGAGTGGC AGACCACTGT CCATACCCTA AAACAAAACT CCAGCATTTC AGGAAGCATC 
AATTTGAGAA CAGATTTAAA CTACATCCAG GCCCTGAGTC GCTTTGAGGA AGGTGATCTT 
GCTGAGTGTG CAACTCAGCT TGAGCTGCTG CCTGGAGAGG ACTATAGTTT GCTCTCTTAC 
AGCAAAGACA AACTGGACCT TAAGAAACTT CTGCCTTCCA TGAGTCCGGA TCCAATTGAA 
CTGCAAAGAG CCATTGAAGT GCAGCTCCTT CGCAGTGCTG TTAGTTCCAC GCCCAAAGCC 
ACACACCAGG AGCCAAAAAG TACCCCAAGT CCCGACATGT TGCTACGATG CCTGAAGCAG 
ACTGGAAGAC TCTGTTTGGG TCCTCTCCGT TTGTCTACCC TCAATGTGGC TGACAACTTG 
CCCACCCTGC CAACCCTGCA GCTACATTGC AGTTCCATGC TGGAGAACAC ACTCATAGGA 
CAGGAGGAAT GTGTGATTCC TCTATCCAGT GAGGCTTTAA GGTCTTGTAA ACAACAGGAT 
GTCCAGCCTT TTCTTCATGC TTTCAGATAC ACTGTCTTCC AAAGACAGCA CCTTAATAAG 
GTTAAAGGCT ACTCTTCATC GACAGATGGC CATCTAACGG AGCTTTGCTT GACGGCTGTC 
AAATTTGCCA GGAAAACAGG AAACATTAGC CTAGCTTCCC GTTTGATTAG GCAGTGTGGT 
GATAGAGCCC ATGAGGAGGA GGAGAAGGGT GATTTGAGCC TCACATTCAG GCGACTCAAT 
CTGGAGGGCA CTCTTGGCGA GAAATGGGGA CCAGAGCTAC AGATAGAAAA AGCTAAAGTT 
CTTAGAGCTG CAGGCCAGTC CATGGCAGCC ATGGAGATGC TAAGTGGAGC TGCAATGTCA 
TACTGCCACA TGGGGAAGAA TGAAGGGGCT GCCTGCCGCT CACTGCTCAC ACTTTGCAAA 
TGGTTGCTGG CTGACTGGAA AGACATGAGT TCTCAACTTA AACAGGTGAT AAAGAAGTCA 
GGTGTAGTGA ACTCCAGTGC CGGCAATATA ACATCCCTCA CCAGAAACAT TTCTTCACTG 
CTGGAACTTT CCCTCAAAGA GCAGGGTATT CCCCACATCA CCGCTGAGAC CTCAGTAAAT 
GTGGGTGTTG GGGAGCCAGA CTTTGTTTTG GGCCAGCTCT ACCAGCTGTC TACATACCTG 
GCTCCAGAGA TGGCAAAGTC CTGGGCAGCT CTGGCTAGTT GGGCTTATCG CTGGGGAAGG 
AAGGTGGTGG ATAATACCAG ATTCTCAGGC ACATCCCTAC CAGCAGCAGC TTCACCTATT 
AGTTTTTTTT TTTGTCTATA TATTTATTAT ACTTTCAGCC ATGGTGAAGG TTTGCCTCTT 
CTACCTGAAG AGAAAAGAGA GATAGAAGAG CTGCTTCCAT CAACTACCAA TGACGAAGAT 
AAAGAGAAAA TTTTCAGCAT TCTCGGCCAG GCCATGTGTA GACCTACTGG TATACAGGAT 
GAAGACATTG CTCTCCAGCA GGATGATGAT GAGGATGACA TGGTGGATGT GATCTGGAGA 
CAACTCACTG GCGCATGTCC CTGGCTTGCA AATATGGGCC AGGGTGTCTC AGATGGTCTG 
ATTCAGGTTT GGAGACATGT GGTGGATCGC ATCTTTGGCC TCTACAGGGT CTCATGCCAG 
GCCTATTTTA CTTTTTTGAA GCTCAATGTT GGACAGGTCC CTTTAGATGA AGATGATCCA 
AAAGCCTTGC TGTCATTTCA AAACAACAAA CAGAGCAATG ATGATGTGAT TGTCATGGCA 
ACCCTGCGGC TCCTTCGATT GCTGGTGAAG CATGCTGGTG AATTGAGAGA AGTACTGGAG 
TTGGGTTTAG CCTCGACGCC TACAGGACCC TGGAGAGGGA TCATCCCCCA GCTTTTTTCC 
CGTCTCAATC ATCCAGAGGC ATATATCCGG CAAAGCATAT GCAGTTTGCT GTGTCGAGTG 
GCCCAAGACT CCCCGCATCT CATACTCTAC CCTGCCATTG TGGGCTCACT ATCCTTTGGA 
ACAGAAGCCC AGAATGCAGG AAATCAACTA CCTTCAGCAC TTCCCACGTT TTTGGGAAGT 
ATGCAGGGAG AGGGCATTGT AGGAGAGGAG CAGGCCGATG GCAACGGACC AGATGGAGCG 
CCAAAAGCAG AACTAGCAAA CTTGTACTCC AGTCAAGACC AAGCCATGAT GCAGGATTGT 
TACAGCAAGA TAGTGGAAAA ACTGTCCATT GCAAATCCTG CCATGGTGCT TCAGGTCCAA 
CAGCTGGTGG GTGAACTACG CAGAGTTACC TTACTCTGGG ATGAATTGTG GCTCGGTGTG 
TTGCAACAGC AACATATGCA TGTCCTTCGT AGGATCCAGC AGCTCGAGGA TGAGGTTAAG 
AGGGTTCAGA ATAACGCCAC GCTGCGCAGG GATGAAAAGA TTGCTATCAT GCGGGAAAAA 
CACTCTGCTT TGATGCGTCC TGTGGTTTTT GCTCTGGATC ACGTTTGCAG TATCACTGCA 
GCCCCTGCAG AGACACCACA TGAAGTGTGG TTCCAGCAGA CTTACGGGAG TGATATCATA 
TTTGCCCTTG AACGACTGAG AAACCCTAAT AACCCCGCCA ACCCTGCCAG CAGCTGGCTT 
CCCTTTAAAC AGATCATGTT GAGCCTGCAG CAGCGTGCCC AAAAGCGAGC TAGCTACCTG 
CTTCACCTGG AGGAGATCAG TCCTCATCTT GTGTCAATGA CGAAGACAGA AATGGCCTTG 
CCTGGTGAGG TGTCAGCATC AGACGCTATC ACCATACAAA GCATAGGCAA TACCATTACC 
ATTCTACCCA CTAAGACCAA GCCCAAAAAG CTGTTCTTCA TGGGCTCAGA TGGACGACAT 
TACCCATACC TCTTTAAAGG TCTGGAGGAT TTACATTTGG ATGAGCGCAT CATGCAGTTT 
CTGTCCATTG TCAATACCAT GTTCACCAAG ATTAACCAGC AAGAGCAGCC TCAATTCCAT 
GCCTGCCACT ACTCTGTCAC CCCGCTTGGA ACCAGGTCAG GGCTTATTCA GTGGGTGGAT 
GGAGCCACAC CACTGTTTGG CCTCTACAAA CGCTGGCAGC AAAGGGAGGC TGTGCTCCAG 
ACCCAGAAGG GCCAAGATTC ATTGCAGCAA CAGCTGCCAA TCCCTCCAGT ACCTCGCCCA 
AGTGAACTGT ACTACAGCCG CATTGGGCCA GCTCTGAAGG CAGTGGGGCT CAGCCTGGAC 
GTGACGCGAC GAGATTGGCC TCTAAGTGTC ATGAAAGAGG TGCTAAAAGA GTTGATGGAG 
GCTACACCTT CCAACTTGCT TGCTAAAGAG CTGTGGTGTT CCTGCACCAC GCCAAGTGAG 
TGGTGGAGAG TAACTCAGTC TTATGCCAGG TCCACTGCTG TCATGTCGAT GGTTGGCTAT 
ATCATTGGCC TTGGTGACCG TCATCTAGAC AATGTGTTGA TTGACATGAC CACAGGAGAG 
GTGGTGCACA TTGACTACAA TGTATGCTTT GAGAAAGGGA AGAGTCTACG AGTGCCAGAG 
AAAGTCCCAT TCAGGATGAC CCACAACATT GAGACTGCTC TGGGTGTCAC CGGTGTGGAG 
GGCATCTTCA GGCTGTCCTG TGAACAGGTT CTTCAGATCA TGCGTCGTGG CAGAGAGACC 
CTGTTGACCC TGCTGGAGGC CTTTGTCTAC GATCCTCTTG TGGACTGGAC TGCTGGTGGG 
GAAGTGGGTT TTGCTGGCGC TGTTTATGGA GGAGGAGGCC AACAAGCTGA AAACAAGCAG 
AGCAAAAGGG AGATGGAAAG GGACATTACA CGCTCTCTTT TCTCATCCAG GGTAGCTGAG 
ATTAAGGTGA ATTGGTTTAA AAATCGTGAT GAAATGCTGG CAGTGTTGCC ACAAGTGGAA 
GAGGCTGTGG AAGAGTACCT GGTTCTGCAG GAGCTGCTCT GCCAGGGTGG GAAACTGCAG 
GACAAACTTC AAGAAGAGAT AACCTTCCTA GAAGGAGCAG AGAACCATCC AGACCATCTT 
ATTCTTACCC TGGAGCAAAG GTACTCTGAA CACACTCGGC TTCAGTCACA GCAGCGAACG 
ATGCAGGAGG CCATTCAAAG CAAACTCACT GACCTTGACC AGTGGATTTC CCAGTACCAG 
GCAGCTTTCT CCAACCTGGA AGCTGCCCAG CTGGCCACTT TGCTTCAAGA CATCAGTTGC 
CCCATAGATT TGGGTCCTCC CAGCTATGTA CCTGCTACAG CCTTCCTACA GAATGCAGGC 
CAGGCCCATT TGATCACCCA GTGTGAGAAC TTGGAAGCAG AATTGAGCAC TTTGCTGCAG 
CAGCGACGTG GGGCCCTTCG TGGTTGCCTA GAGCAGTTGC ACAGCTACGC AACAGTAGCA 
TTACTGTACC CAAGAGGTAC TTTGCGGCTC CATCGAGTCC AGCAGTGGAA GGCCTGGTTA 
GAGGAATTGT TGACAGACAT GACGGTGGAG CAGTGCCAGC ATATCTACCG ACAATATGAG 
ATACTCTTTG CACCTCAGCC TTCTGCAGCT ATCTGTCAGT TTTTGTCAAG TGTGGAGTTG 
ACTTTGCAAC ATCAGATGGC AGAAACCAAT GATCGTGTGA TGCATCAGGC AGAGCGGCTC 
AAGGCCGAGG GAACGACTGT GGCAGTGTGC GAAGATCAAG TACAGGAGAT TGACCACTGT 
ATCACAGGTT TTCTACATGA GAACAGAGAG TATGGTTCTC TGGGATTGGC AGGAATCATC 
ACCTCTCTCC TGTGTACACT GTGCAGAAGA AATCTGGTTA TGGAAACTGC TGCAGCCAAT 
GCTGGAGAAC AGCTTATTGA CTTAAAGTCT CGAGATGGGA CATGGTTCCT GGAGGAGCTC 
TGCAGTATGT TTGGAAATGT CAGTGGCCTG ACAACACTTC TACAACGCTG CCAACTTGTA 
CAACATGATC TGGAGCTGCC TGCCCTCTCA GACACCATCC AAGCTGTTTA TCTGGCCAAT 
GTTGTATATA CCTGCTTACA AGAACTAAAC ACCAATTTTC GACAGATCAT CTTTCCAGAA 
GCTTTGTGTT GTATGATAAA AGGAGAGCCC ACTCTCGAGT CCATGTTGGT GGAGATAGAG 
CAGCTGGTGG AGCAGAGCTC TGATGGTCTT GGACTGCAGG GATTGGTTGA CAGTCTGCAA 
TCTACTATGC GGTTCGACCA AGATGGACAC AACCATTTGC TTCACATTAC AAGTTTACTG 
CGTGCTCAGT ACTCTGGGCT GATCCAGCCT CGGAACACAG AAGGACCAGG TCACGACACA 
CCAAAGATGT CCCCAGGACA AATGTTGTTG GTGGCGTTTG ATGGAATGTT CACCCAGCTA 
GAGAATGCAT TTATGCAGCT CACAGAGAAG CTGAGTTGTC TCGAGGTGCC TTCAGCCTGG 
CAAAAAATAG ATTCTTTGAG GGAAGCTCGG ACCGCACAGC TCTATTTCTT TGAGAATCGG 
AGTCAGAGAC AACTCATGGA AGAGGTATTC TTCCTCAAAA GACTGCAGAC CATTAGGGAT 
TTCTTCAAGC TTTGTGCCAC ATTTGCCCAA ACTATCTCTG GTACCTGTCC TTCAACTGAG 
GACCTCTTAA CAAATGGGCC AGTAGGATCG GGGAAGTCAT TCTACCAGCT GCCCACTGCA 
TCAGACTTGG GTGTGCCAGT GGTCAGTGAA GAAAAGATGA CCCATCCGAT TAAGGCCTTC 
ACAGCTGAGT TTGTCCGACA GATGCTCATG GGTCTGCCCA GCCAAACACT AAGCCTAGTT 
CTGTGCAGCG CCCTTTCTTC TCTTGGGCTG GATATTGTTT CCCTGGTGGA GGCCAAAGAC 
TTTGGTGTTG AGAGAAAGGT TTCTCTGGAT GAACTTTGTA AGACGGCAGT GGAGCAGAGC 
CTAGCTTCAG GCCGGGTTTC CCAGCTGTTG TTGAACAGAG CCACTGTATT GGCCTCCTCC 
TATGATACAG CTTGGAAGAA ACTAGACCTT CTCAGAAGAC TGGATGCCCA CCTGGAGGCT 
GCCAAGGCCA GTTTGCAGCG AAGCCAGCTG CATATCGCCA TGTTTCAGTG GCATCATGAG 
GATATTCTGG GCCCCAGAAA TCAGCCTCTA GGTGTAAGCA TTCCACCTCG TACCATCATT 
CTTAGCAACA TGAAGAAAAA ACTTTACAAG CTGAGTCAGG ATGAGGGAGC CATAGTTGCT 
GTTCAGGAGA AGCTGGCGTC ACTGGAGGCT AGCATTGAGC AACGACTAAA GTGGGCAGGT 
GGAGCCAATC CAGCACTGGC CCCAGTACTG CAAGACTTTG AGATGACCAT CACTGAGAGA 
CGTGCAATTG TGGCCAGAGA AAACCAGCGC ACCAGCCAGG TCACTTTTCT GTGCTCAACT 
ATCCTTAATT TTGAAGGCCT TCGTACCCGA ACACCTGAGG CCCTGAATAT GGATGCAGCA 
CTTTTTGAGT TGTTAAAACG TTGCCAGCAA ACCTGCTCCT TTGCTGCCCA GTTTAGCAGC 
AGTGTGTCTC CACTTGAGCT TCAGCTGTTG CAAAGGCTGA GTCCAGTGCT TGACTTGCCT 
ATTGGCAGCC CCGACTGGCT GAGCTGTGCA CAAAAGCAAC TGGAGGAGCA GCTTAATGTT 
GAGCAAAGCA CTCGGGAGGA GCGGGAACAG CAGCTGGAGG TCTGTGCAGA GACTCTGCAA 
GTTCTGGTCG ATTCTGTAAA GACCATCCTG TCCCATCACA ACCGCCAGCT GGCAGATGTC 
AAGCACCTAT TGAGAGCCAT GGCAAAGGAC GAGGAGGCAG CTTTGGCAGA TGGTGAAGAG 
GTGATATATG AGGGTAGCGT GCGTCAGTTT CTGCTGGAGT ACAAAGCATG GCAAGACAAC 
ATCCAGCTGG TTTTATTCAC TGTGGTCCAG GCTAGTGGAC AGCCTCGCAG TCAGGAGCAG 
CTTGAGCTGC TGGAAGACAT TCCTGACACG CTGAAGGAAC TGTACTCTCA AAGTGAGAGT 
GTGTATAATG GCTTAGTTGG CTTCGCTTCC CCACTCGTGA CTGACCGTGA AAGTGAAAGC 
ACCAGTCCAG TGTCTGCAAT ACAGACGAGC TTTGCAGCAG CTGTCAGGTG CACTGGGTTA 
AAGACCCAGC CAGACTCGGT CTCCCAGAAT GTTCGCAAGG CTCTGCCGCG GAATCTCGGA 
ACCCCTACTG ATACCCCGCC CAGTGCTCCG TTGGTGAACA ACAAGTGTCT CAATGCCAGC 
CCAAAGAGAG CCATACGTGA CCCCAAAACT GGACGAGCTG TTCAGGAGAG AAACTCCTAT 
GCAGTCAGTG TGTGGAAGCG AGTGAAAGCC AAACTTGAGG GAAGAGATAT TGATCCGAAC 
AGAAGGATGA GTATCACAGA GCAGGTGGAC TTTGTCATCA AAGAGGCCAC AAATCTGGAC 
AACCTTGCGC AGCTATACGA AGGCTGGACT GCCTGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029695595.1
CDS	142..11163
Translation	MSRKSSGSRLSGAHKLQPHWNDWQPRTDSASLSQEGELDSSILYDILPSSCPDPPVVSSNAFHAADTHQEEGGYEGNKVICINAFGWKSVGQELRPNDLTTDLPRFQHGNRALASKDMKKSQDRGMGHSEESRLANLLRRVSREDDRDRRLATLRQLKDFISHSESKVTLVKQLDTILSTLNDILNESSKLLWELRQDAAICLGLLCTVLSYEAESIFKWLFLKLTSSNRDEVKLLYLMATYHALEAAGERKAFSHVMQLVMTNLQSILENVDTPELLCQSVKCILQVAHCYPHVFSINFRDTVDILVGWHIDHTQKQSVTQQVSGWLQSLEQFWVADLTFSTTLLGQFLEDMEAYAEDLNHVVLGEILDEDIPPPSVSLPKLAALLRVFSTVVRSIGERFSPLRGPPITEVYVTDVLNRVLACVSTAKQVQFSEEVLTAGNECVGVLLASVEPSGQLMEAVLVYGLDQLDCCQACSSDYNLSVLIILTMIVDQINTKLPASFVEKLLAPESKLMQLRFHRDKEVISAVHGVYQALLSLKNIPTLEAAYKMVLGEIACALSSLTLVLKANGMAPAPNGCLYSNIQHPAFTSLTLPLENAEFTLIFNLSTLTTIGNTKNSLIGMWALSPTVFTLLSQNLLLVHSDLAVYYPTIQYAVLYTLYSHCTRHDHFISSSLSSSPSLFDGAVISTVTTATKKHFSTLLGLLGGLLLKEHLYIETRRLLLTWAQEISVLMKKSDTYSPLFSLTTFNKFCRGLLANALNEDQSICLQTCHCIMVLSSSLSTELLQSCVDVCRVQLVNSAVRVRQAYGKLLKTIPLDVALSVQSQPRMKDISLAVRHHMSRAPSSTFHPQDFSDLISFILYGTLHRGGKDPWLERLYYSCQRFEKLDGRGCADSGKPGVVPQALLKNEAVLWQWAAWEAAQFTVLSKLRTPLGRAPDTFQTIEGMIRSLAAHAVNPEQDVGAWAGSGGDGDGHHSNQLRLALLLQYLENLEKLMYNAYEGCANALTSPPKSIKTFFYTNRQTCQDWLTRIRLALMRVGLLSGKPAVTIRHGFDLLTEFKNSSAQGPEVEVLLTLLAEALCELNCPEAIQGLAAWSLLTSGRSLGWLSSVALQAEGRFEKAALEYQDHLSAVTGMDCSIKSSDCFLPKFSNSTSSPKHVNNGDNKKTVLLKGNECSSEVINFLVNKASQCYVALCDWASVKEWQTTVHTLKQNSSISGSINLRTDLNYIQALSRFEEGDLAECATQLELLPGEDYSLLSYSKDKLDLKKLLPSMSPDPIELQRAIEVQLLRSAVSSTPKATHQEPKSTPSPDMLLRCLKQTGRLCLGPLRLSTLNVADNLPTLPTLQLHCSSMLENTLIGQEECVIPLSSEALRSCKQQDVQPFLHAFRYTVFQRQHLNKVKGYSSSTDGHLTELCLTAVKFARKTGNISLASRLIRQCGDRAHEEEEKGDLSLTFRRLNLEGTLGEKWGPELQIEKAKVLRAAGQSMAAMEMLSGAAMSYCHMGKNEGAACRSLLTLCKWLLADWKDMSSQLKQVIKKSGVVNSSAGNITSLTRNISSLLELSLKEQGIPHITAETSVNVGVGEPDFVLGQLYQLSTYLAPEMAKSWAALASWAYRWGRKVVDNTRFSGTSLPAAASPISFFFCLYIYYTFSHGEGLPLLPEEKREIEELLPSTTNDEDKEKIFSILGQAMCRPTGIQDEDIALQQDDDEDDMVDVIWRQLTGACPWLANMGQGVSDGLIQVWRHVVDRIFGLYRVSCQAYFTFLKLNVGQVPLDEDDPKALLSFQNNKQSNDDVIVMATLRLLRLLVKHAGELREVLELGLASTPTGPWRGIIPQLFSRLNHPEAYIRQSICSLLCRVAQDSPHLILYPAIVGSLSFGTEAQNAGNQLPSALPTFLGSMQGEGIVGEEQADGNGPDGAPKAELANLYSSQDQAMMQDCYSKIVEKLSIANPAMVLQVQQLVGELRRVTLLWDELWLGVLQQQHMHVLRRIQQLEDEVKRVQNNATLRRDEKIAIMREKHSALMRPVVFALDHVCSITAAPAETPHEVWFQQTYGSDIIFALERLRNPNNPANPASSWLPFKQIMLSLQQRAQKRASYLLHLEEISPHLVSMTKTEMALPGEVSASDAITIQSIGNTITILPTKTKPKKLFFMGSDGRHYPYLFKGLEDLHLDERIMQFLSIVNTMFTKINQQEQPQFHACHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAVLQTQKGQDSLQQQLPIPPVPRPSELYYSRIGPALKAVGLSLDVTRRDWPLSVMKEVLKELMEATPSNLLAKELWCSCTTPSEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTHNIETALGVTGVEGIFRLSCEQVLQIMRRGRETLLTLLEAFVYDPLVDWTAGGEVGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRVAEIKVNWFKNRDEMLAVLPQVEEAVEEYLVLQELLCQGGKLQDKLQEEITFLEGAENHPDHLILTLEQRYSEHTRLQSQQRTMQEAIQSKLTDLDQWISQYQAAFSNLEAAQLATLLQDISCPIDLGPPSYVPATAFLQNAGQAHLITQCENLEAELSTLLQQRRGALRGCLEQLHSYATVALLYPRGTLRLHRVQQWKAWLEELLTDMTVEQCQHIYRQYEILFAPQPSAAICQFLSSVELTLQHQMAETNDRVMHQAERLKAEGTTVAVCEDQVQEIDHCITGFLHENREYGSLGLAGIITSLLCTLCRRNLVMETAAANAGEQLIDLKSRDGTWFLEELCSMFGNVSGLTTLLQRCQLVQHDLELPALSDTIQAVYLANVVYTCLQELNTNFRQIIFPEALCCMIKGEPTLESMLVEIEQLVEQSSDGLGLQGLVDSLQSTMRFDQDGHNHLLHITSLLRAQYSGLIQPRNTEGPGHDTPKMSPGQMLLVAFDGMFTQLENAFMQLTEKLSCLEVPSAWQKIDSLREARTAQLYFFENRSQRQLMEEVFFLKRLQTIRDFFKLCATFAQTISGTCPSTEDLLTNGPVGSGKSFYQLPTASDLGVPVVSEEKMTHPIKAFTAEFVRQMLMGLPSQTLSLVLCSALSSLGLDIVSLVEAKDFGVERKVSLDELCKTAVEQSLASGRVSQLLLNRATVLASSYDTAWKKLDLLRRLDAHLEAAKASLQRSQLHIAMFQWHHEDILGPRNQPLGVSIPPRTIILSNMKKKLYKLSQDEGAIVAVQEKLASLEASIEQRLKWAGGANPALAPVLQDFEMTITERRAIVARENQRTSQVTFLCSTILNFEGLRTRTPEALNMDAALFELLKRCQQTCSFAAQFSSSVSPLELQLLQRLSPVLDLPIGSPDWLSCAQKQLEEQLNVEQSTREEREQQLEVCAETLQVLVDSVKTILSHHNRQLADVKHLLRAMAKDEEAALADGEEVIYEGSVRQFLLEYKAWQDNIQLVLFTVVQASGQPRSQEQLELLEDIPDTLKELYSQSESVYNGLVGFASPLVTDRESESTSPVSAIQTSFAAAVRCTGLKTQPDSVSQNVRKALPRNLGTPTDTPPSAPLVNNKCLNASPKRAIRDPKTGRAVQERNSYAVSVWKRVKAKLEGRDIDPNRRMSITEQVDFVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_029839735.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes SMG1 nonsense mediated mRNA decay associated PI3K related kinase (smg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029839735.1

ORF Insert Sequence:

ATGAGCCGTA AATCTTCGGG GTCTCGGCTC AGCGGCGCCC ACAAACTGCA GCCACACTGG 
AATGACTGGC AGCCCAGAAC TGACAGTGCG TCACTCAGCC AGGAGGGAGA GCTCGATTCA 
TCCATATTGT ATGATATATT GCCCTCTAGC TGTCCCGATC CTCCAGTGGT GTCTAGCAAT 
GCCTTTCATG CTGCAGATAC ACACCAGGAA GAGGGTGGAT ATGAAGGCAA CAAAGTTATC 
TGTATTAATG CGTTTGGTTG GAAGTCGGTG GGGCAAGAGC TGAGGCCAAA TGACTTGACC 
ACAGATTTGC CTCGTTTTCA GCATGGAAAT CGTGCTTTAG CTTCAAAGGA CATGAAAAAA 
TCGCAGGACA GAGGAATGGG TCATTCAGAG GAGTCTCGGC TGGCCAACCT CCTGCGTCGA 
GTTTCTAGGG AGGATGATCG TGACCGCAGA CTGGCAACTC TCAGACAACT GAAGGACTTC 
ATTAGCCACA GTGAGAGTAA AGTGACACTG GTTAAACAAT TGGACACAAT CCTCAGCACC 
CTAAATGATA TTTTAAATGA GAGTAGTAAG CTACTCTGGG AGCTGCGTCA GGATGCAGCC 
ATTTGCCTTG GTCTACTCTG CACGGTTCTC AGCTATGAGG CTGAGAGCAT CTTCAAGTGG 
CTATTTCTAA AATTAACATC GAGCAATCGC GATGAAGTGA AGCTGTTGTA CCTTATGGCA 
ACATATCACG CATTGGAGGC TGCTGGAGAG AGGAAGGCCT TTTCTCACGT CATGCAGCTG 
GTGATGACCA ATTTGCAGTC CATCCTGGAG AATGTTGACA CACCAGAGCT GCTTTGTCAG 
AGTGTGAAGT GTATTCTTCA GGTGGCGCAC TGTTACCCAC ATGTGTTCAG CATCAACTTT 
AGAGATACAG TTGATATATT GGTGGGCTGG CACATAGACC ACACTCAGAA GCAGTCGGTC 
ACACAGCAAG TTTCAGGTTG GCTTCAGAGT CTGGAACAGT TCTGGGTGGC GGACCTGACA 
TTCTCCACCA CACTACTGGG ACAGTTCCTG GAGGATATGG AAGCATATGC AGAGGATCTG 
AACCATGTTG TTTTAGGGGA GATTCTCGAT GAAGATATTC CACCTCCCTC GGTGTCACTG 
CCAAAGTTAG CAGCACTGTT GAGAGTTTTC AGCACAGTGG TCCGCAGCAT TGGAGAGCGA 
TTTAGTCCCC TAAGAGGACC ACCCATTACG GAGGTCTACG TCACAGATGT TCTGAACCGT 
GTTCTGGCCT GTGTGTCGAC GGCAAAGCAG GTTCAGTTTT CTGAAGAAGT TCTCACTGCT 
GGAAACGAGT GTGTGGGTGT GTTGCTAGCT AGTGTGGAAC CCAGTGGTCA GTTGATGGAG 
GCTGTCTTGG TCTATGGGCT GGACCAGTTG GACTGCTGCC AGGCCTGCAG CTCAGACTAC 
AACCTTTCAG TGCTCATCAT CCTCACTATG ATAGTGGACC AAATCAACAC AAAGCTCCCA 
GCATCATTTG TTGAAAAACT GTTGGCTCCA GAATCCAAGT TGATGCAGTT GCGGTTTCAC 
AGAGATAAAG AGGTGATTTC AGCTGTTCAT GGTGTGTATC AGGCATTGTT AAGCCTGAAG 
AACATTCCTA CACTTGAAGC AGCATATAAA ATGGTATTGG GTGAGATAGC ATGTGCCCTC 
TCCAGCCTAA CTTTGGTGCT GAAAGCCAAT GGTATGGCTC CTGCCCCCAA TGGCTGCCTC 
TACTCAAATA TCCAACATCC TGCCTTTACT TCTCTCACCC TACCTTTGGA AAATGCAGAG 
TTCACACTGA TCTTCAACCT CAGCACCCTC ACTACCATTG GTAACACCAA GAATTCTCTG 
ATTGGGATGT GGGCCTTGTC TCCTACAGTC TTCACACTGC TCAGTCAAAA TCTTTTGTTG 
GTCCACTCTG ACTTAGCAGT GTACTACCCC ACCATCCAGT ATGCCGTACT TTACACACTG 
TACTCCCACT GCACAAGACA TGATCATTTC ATCTCATCCA GCTTATCATC TTCTCCATCA 
CTATTTGATG GTGCTGTTAT CAGCACTGTG ACCACAGCAA CAAAGAAACA CTTCAGCACA 
CTCTTAGGCC TTCTTGGAGG TCTCCTGTTG AAAGAACATC TCTACATTGA GACCAGGAGA 
CTGCTGTTGA CCTGGGCTCA GGAAATCTCG GTGCTGATGA AAAAGTCAGA CACTTATAGC 
CCTCTCTTCT CTTTGACCAC TTTCAACAAG TTTTGCAGGG GTCTCCTTGC CAATGCTCTA 
AATGAAGATC AGAGTATTTG TCTTCAGACC TGCCACTGTA TTATGGTTCT GTCTTCATCT 
TTGTCCACTG AGCTGCTGCA GAGCTGTGTG GATGTGTGTA GAGTACAGTT GGTCAACTCA 
GCAGTAAGAG TAAGGCAGGC CTATGGTAAG CTGCTCAAGA CCATTCCACT GGATGTGGCT 
TTGAGTGTCC AAAGCCAACC CAGGATGAAG GATATTTCCC TGGCTGTCCG CCACCACATG 
AGTCGAGCAC CAAGCAGCAC CTTTCATCCC CAGGACTTCA GTGACCTCAT TAGTTTCATT 
TTGTATGGGA CCTTGCACCG TGGCGGTAAG GATCCCTGGC TGGAGCGATT GTATTACAGC 
TGTCAGCGTT TTGAGAAGCT GGATGGCCGA GGCTGCGCTG ACAGTGGTAA GCCAGGTGTT 
GTACCACAAG CCCTGCTGAA AAATGAGGCT GTGCTGTGGC AGTGGGCAGC GTGGGAGGCT 
GCACAATTCA CTGTTCTGTC CAAACTCCGA ACGCCACTGG GCCGTGCTCC AGACACCTTT 
CAAACCATTG AAGGTATGAT CCGTAGCCTG GCTGCACATG CTGTCAACCC CGAACAGGAC 
GTCGGAGCGT GGGCTGGATC TGGGGGTGAT GGGGATGGTC ACCACTCCAA TCAGCTCCGT 
CTTGCTCTGT TGCTTCAGTA CCTGGAAAAC CTGGAGAAAT TAATGTACAA TGCATATGAA 
GGATGTGCCA ATGCTCTAAC TTCTCCTCCA AAGAGTATCA AAACTTTCTT TTACACAAAC 
CGTCAGACAT GCCAAGATTG GCTAACAAGG ATCCGTCTTG CTCTGATGAG AGTTGGTCTT 
CTTTCTGGCA AACCAGCAGT CACCATTCGC CATGGATTTG ACTTGCTAAC AGAGTTTAAG 
AACAGTAGTG CTCAGGGTCC AGAGGTGGAG GTCCTTCTGA CATTGTTGGC AGAAGCTCTC 
TGTGAGCTAA ATTGTCCTGA GGCCATACAG GGTTTGGCAG CATGGAGCCT TTTGACCAGT 
GGTAGAAGTC TTGGCTGGCT GTCATCAGTC GCCCTACAGG CTGAGGGAAG GTTTGAGAAG 
GCAGCTCTTG AATATCAAGA TCACCTCTCT GCAGTTACAG GGATGGACTG TAGCATCAAA 
AGCAGTGACT GCTTTTTGCC TAAATTCTCC AACAGCACAA GTAGCCCCAA ACATGTGAAC 
AATGGTGACA ATAAGAAGAC GGTGCTCCTC AAGGGAAATG AGTGTTCCTC TGAGGTCATC 
AACTTTCTGG TCAACAAAGC TAGTCAGTGT TATGTGGCGC TCTGTGACTG GGCATCAGTT 
AAAGAGTGGC AGACCACTGT CCATACCCTA AAACAAAACT CCAGCATTTC AGGAAGCATC 
AATTTGAGAA CAGATTTAAA CTACATCCAG GCCCTGAGTC GCTTTGAGGA AGGTGATCTT 
GCTGAGTGTG CAACTCAGCT TGAGCTGCTG CCTGGAGAGG ACTATAGTTT GCTCTCTTAC 
AGCAAAGACA AACTGGACCT TAAGAAACTT CTGCCTTCCA TGAGTCCGGA TCCAATTGAA 
CTGCAAAGAG CCATTGAAGT GCAGCTCCTT CGCAGTGCTG TTAGTTCCAC GCCCAAAGCC 
ACACACCAGG AGCCAAAAAG TACCCCAAGT CCCGACATGT TGCTACGATG CCTGAAGCAG 
ACTGGAAGAC TCTGTTTGGG TCCTCTCCGT TTGTCTACCC TCAATGTGGC TGACAACTTG 
CCCACCCTGC CAACCCTGCA GCTACATTGC AGTTCCATGC TGGAGAACAC ACTCATAGGA 
CAGGAGGAAT GTGTGATTCC TCTATCCAGT GAGGCTTTAA GGTCTTGTAA ACAACAGGAT 
GTCCAGCCTT TTCTTCATGC TTTCAGATAC ACTGTCTTCC AAAGACAGCA CCTTAATAAG 
GTTAAAGGCT ACTCTTCATC GACAGATGGC CATCTAACGG AGCTTTGCTT GACGGCTGTC 
AAATTTGCCA GGAAAACAGG AAACATTAGC CTAGCTTCCC GTTTGATTAG GCAGTGTGGT 
GATAGAGCCC ATGAGGAGGA GGAGAAGGGT GATTTGAGCC TCACATTCAG GCGACTCAAT 
CTGGAGGGCA CTCTTGGCGA GAAATGGGGA CCAGAGCTAC AGATAGAAAA AGCTAAAGTT 
CTTAGAGCTG CAGGCCAGTC CATGGCAGCC ATGGAGATGC TAAGTGGAGC TGCAATGTCA 
TACTGCCACA TGGGGAAGAA TGAAGGGGCT GCCTGCCGCT CACTGCTCAC ACTTTGCAAA 
TGGTTGCTGG CTGACTGGAA AGACATGAGT TCTCAACTTA AACAGGTGAT AAAGAAGTCA 
GGTGTAGTGA ACTCCAGTGC CGGCAATATA ACATCCCTCA CCAGAAACAT TTCTTCACTG 
CTGGAACTTT CCCTCAAAGA GCAGGGTATT CCCCACATCA CCGCTGAGAC CTCAGTAAAT 
GTGGGTGTTG GGGAGCCAGA CTTTGTTTTG GGCCAGCTCT ACCAGCTGTC TACATACCTG 
GCTCCAGAGA TGGCAAAGTC CTGGGCAGCT CTGGCTAGTT GGGCTTATCG CTGGGGAAGG 
AAGGTGGTGG ATAATACCAG ATTCTCAGGC ACATCCCTAC CAGCAGCAGC TTCACCTATT 
AGTTTTTTTT TTTGTCTATA TATTTATTAT ACTTTCAGCC ATGGTGAAGG TTTGCCTCTT 
CTACCTGAAG AGAAAAGAGA GATAGAAGAG CTGCTTCCAT CAACTACCAA TGACGAAGAT 
AAAGAGAAAA TTTTCAGCAT TCTCGGCCAG GCCATGTGTA GACCTACTGG TATACAGGAT 
GAAGACATTG CTCTCCAGCA GGATGATGAT GAGGATGACA TGGTGGATGT GATCTGGAGA 
CAACTCACTG GCGCATGTCC CTGGCTTGCA AATATGGGCC AGGGTGTCTC AGATGGTCTG 
ATTCAGGTTT GGAGACATGT GGTGGATCGC ATCTTTGGCC TCTACAGGGT CTCATGCCAG 
GCCTATTTTA CTTTTTTGAA GCTCAATGTT GGACAGGTCC CTTTAGATGA AGATGATCCA 
AAAGCCTTGC TGTCATTTCA AAACAACAAA CAGAGCAATG ATGATGTGAT TGTCATGGCA 
ACCCTGCGGC TCCTTCGATT GCTGGTGAAG CATGCTGGTG AATTGAGAGA AGTACTGGAG 
TTGGGTTTAG CCTCGACGCC TACAGGACCC TGGAGAGGGA TCATCCCCCA GCTTTTTTCC 
CGTCTCAATC ATCCAGAGGC ATATATCCGG CAAAGCATAT GCAGTTTGCT GTGTCGAGTG 
GCCCAAGACT CCCCGCATCT CATACTCTAC CCTGCCATTG TGGGCTCACT ATCCTTTGGA 
ACAGAAGCCC AGAATGCAGG AAATCAACTA CCTTCAGCAC TTCCCACGTT TTTGGGAAGT 
ATGCAGGGAG AGGGCATTGT AGGAGAGGAG CAGGCCGATG GCAACGGACC AGATGGAGCG 
CCAAAAGCAG AACTAGCAAA CTTGTACTCC AGTCAAGACC AAGCCATGAT GCAGGATTGT 
TACAGCAAGA TAGTGGAAAA ACTGTCCATT GCAAATCCTG CCATGGTGCT TCAGGTCCAA 
CAGCTGGTGG GTGAACTACG CAGAGTTACC TTACTCTGGG ATGAATTGTG GCTCGGTGTG 
TTGCAACAGC AACATATGCA TGTCCTTCGT AGGATCCAGC AGCTCGAGGA TGAGGTTAAG 
AGGGTTCAGA ATAACGCCAC GCTGCGCAGG GATGAAAAGA TTGCTATCAT GCGGGAAAAA 
CACTCTGCTT TGATGCGTCC TGTGGTTTTT GCTCTGGATC ACGTTTGCAG TATCACTGCA 
GCCCCTGCAG AGACACCACA TGAAGTGTGG TTCCAGCAGA CTTACGGGAG TGATATCATA 
TTTGCCCTTG AACGACTGAG AAACCCTAAT AACCCCGCCA ACCCTGCCAG CAGCTGGCTT 
CCCTTTAAAC AGATCATGTT GAGCCTGCAG CAGCGTGCCC AAAAGCGAGC TAGCTACCTG 
CTTCACCTGG AGGAGATCAG TCCTCATCTT GTGTCAATGA CGAAGACAGA AATGGCCTTG 
CCTGGTGAGG TGTCAGCATC AGACGCTATC ACCATACAAA GCATAGGCAA TACCATTACC 
ATTCTACCCA CTAAGACCAA GCCCAAAAAG CTGTTCTTCA TGGGCTCAGA TGGACGACAT 
TACCCATACC TCTTTAAAGG TCTGGAGGAT TTACATTTGG ATGAGCGCAT CATGCAGTTT 
CTGTCCATTG TCAATACCAT GTTCACCAAG ATTAACCAGC AAGAGCAGCC TCAATTCCAT 
GCCTGCCACT ACTCTGTCAC CCCGCTTGGA ACCAGGTCAG GGCTTATTCA GTGGGTGGAT 
GGAGCCACAC CACTGTTTGG CCTCTACAAA CGCTGGCAGC AAAGGGAGGC TGTGCTCCAG 
ACCCAGAAGG GCCAAGATTC ATTGCAGCAA CAGCTGCCAA TCCCTCCAGT ACCTCGCCCA 
AGTGAACTGT ACTACAGCCG CATTGGGCCA GCTCTGAAGG CAGTGGGGCT CAGCCTGGAC 
GTGACGCGAC GAGATTGGCC TCTAAGTGTC ATGAAAGAGG TGCTAAAAGA GTTGATGGAG 
GCTACACCTT CCAACTTGCT TGCTAAAGAG CTGTGGTGTT CCTGCACCAC GCCAAGTGAG 
TGGTGGAGAG TAACTCAGTC TTATGCCAGG TCCACTGCTG TCATGTCGAT GGTTGGCTAT 
ATCATTGGCC TTGGTGACCG TCATCTAGAC AATGTGTTGA TTGACATGAC CACAGGAGAG 
GTGGTGCACA TTGACTACAA TGTATGCTTT GAGAAAGGGA AGAGTCTACG AGTGCCAGAG 
AAAGTCCCAT TCAGGATGAC CCACAACATT GAGACTGCTC TGGGTGTCAC CGGTGTGGAG 
GGCATCTTCA GGCTGTCCTG TGAACAGGTT CTTCAGATCA TGCGTCGTGG CAGAGAGACC 
CTGTTGACCC TGCTGGAGGC CTTTGTCTAC GATCCTCTTG TGGACTGGAC TGCTGGTGGG 
GAAGTGGGTT TTGCTGGCGC TGTTTATGGA GGAGGAGGCC AACAAGCTGA AAACAAGCAG 
AGCAAAAGGG AGATGGAAAG GGACATTACA CGCTCTCTTT TCTCATCCAG GGTAGCTGAG 
ATTAAGGTGA ATTGGTTTAA AAATCGTGAT GAAATGCTGG CAGTGTTGCC ACAAGTGGAA 
GAGGCTGTGG AAGAGTACCT GGTTCTGCAG GAGCTGCTCT GCCAGGGTGG GAAACTGCAG 
GACAAACTTC AAGAAGAGAT AACCTTCCTA GAAGGAGCAG AGAACCATCC AGACCATCTT 
ATTCTTACCC TGGAGCAAAG GTACTCTGAA CACACTCGGC TTCAGTCACA GCAGCGAACG 
ATGCAGGAGG CCATTCAAAG CAAACTCACT GACCTTGACC AGTGGATTTC CCAGTACCAG 
GCAGCTTTCT CCAACCTGGA AGCTGCCCAG CTGGCCACTT TGCTTCAAGA CATCAGTTGC 
CCCATAGATT TGGGTCCTCC CAGCTATGTA CCTGCTACAG CCTTCCTACA GAATGCAGGC 
CAGGCCCATT TGATCACCCA GTGTGAGAAC TTGGAAGCAG AATTGAGCAC TTTGCTGCAG 
CAGCGACGTG GGGCCCTTCG TGGTTGCCTA GAGCAGTTGC ACAGCTACGC AACAGTAGCA 
TTACTGTACC CAAGAGGTAC TTTGCGGCTC CATCGAGTCC AGCAGTGGAA GGCCTGGTTA 
GAGGAATTGT TGACAGACAT GACGGTGGAG CAGTGCCAGC ATATCTACCG ACAATATGAG 
ATACTCTTTG CACCTCAGCC TTCTGCAGCT ATCTGTCAGT TTTTGTCAAG TGTGGAGTTG 
ACTTTGCAAC ATCAGATGGC AGAAACCAAT GATCGTGTGA TGCATCAGGC AGAGCGGCTC 
AAGGCCGAGG GAACGACTGT GGCAGTGTGC GAAGATCAAG TACAGGAGAT TGACCACTGT 
ATCACAGGTT TTCTACATGA GAACAGAGAG TATGGTTCTC TGGGATTGGC AGGAATCATC 
ACCTCTCTCC TGTGTACACT GTGCAGAAGA AATCTGGTTA TGGAAACTGC TGCAGCCAAT 
GCTGGAGAAC AGCTTATTGA CTTAAAGTCT CGAGATGGGA CATGGTTCCT GGAGGAGCTC 
TGCAGTATGT TTGGAAATGT CAGTGGCCTG ACAACACTTC TACAACGCTG CCAACTTGTA 
CAACATGATC TGGAGCTGCC TGCCCTCTCA GACACCATCC AAGCTGTTTA TCTGGCCAAT 
GTTGTATATA CCTGCTTACA AGAACTAAAC ACCAATTTTC GACAGATCAT CTTTCCAGAA 
GCTTTGTGTT GTATGATAAA AGGAGAGCCC ACTCTCGAGT CCATGTTGGT GGAGATAGAG 
CAGCTGGTGG AGCAGAGCTC TGATGGTCTT GGACTGCAGG GATTGGTTGA CAGTCTGCAA 
TCTACTATGC GGTTCGACCA AGATGGACAC AACCATTTGC TTCACATTAC AAGTTTACTG 
CGTGCTCAGT ACTCTGGGCT GATCCAGCCT CGGAACACAG AAGGACCAGG TCACGACACA 
CCAAAGATGT CCCCAGGACA AATGTTGTTG GTGGCGTTTG ATGGAATGTT CACCCAGCTA 
GAGAATGCAT TTATGCAGCT CACAGAGAAG CTGAGTTGTC TCGAGGTGCC TTCAGCCTGG 
CAAAAAATAG ATTCTTTGAG GGAAGCTCGG ACCGCACAGC TCTATTTCTT TGAGAATCGG 
AGTCAGAGAC AACTCATGGA AGAGGTATTC TTCCTCAAAA GACTGCAGAC CATTAGGGAT 
TTCTTCAAGC TTTGTGCCAC ATTTGCCCAA ACTATCTCTG GTACCTGTCC TTCAACTGAG 
GACCTCTTAA CAAATGGGCC AGTAGGATCG GGGAAGTCAT TCTACCAGCT GCCCACTGCA 
TCAGACTTGG GTGTGCCAGT GGTCAGTGAA GAAAAGATGA CCCATCCGAT TAAGGCCTTC 
ACAGCTGAGT TTGTCCGACA GATGCTCATG GGTCTGCCCA GCCAAACACT AAGCCTAGTT 
CTGTGCAGCG CCCTTTCTTC TCTTGGGCTG GATATTGTTT CCCTGGTGGA GGCCAAAGAC 
TTTGGTGTTG AGAGAAAGGT TTCTCTGGAT GAACTTTGTA AGACGGCAGT GGAGCAGAGC 
CTAGCTTCAG GCCGGGTTTC CCAGCTGTTG TTGAACAGAG CCACTGTATT GGCCTCCTCC 
TATGATACAG CTTGGAAGAA ACTAGACCTT CTCAGAAGAC TGGATGCCCA CCTGGAGGCT 
GCCAAGGCCA GTTTGCAGCG AAGCCAGCTG CATATCGCCA TGTTTCAGTG GCATCATGAG 
GATATTCTGG GCCCCAGAAA TCAGCCTCTA GGTGTAAGCA TTCCACCTCG TACCATCATT 
CTTAGCAACA TGAAGAAAAA ACTTTACAAG CTGAGTCAGG ATGAGGGAGC CATAGTTGCT 
GTTCAGGAGA AGCTGGCGTC ACTGGAGGCT AGCATTGAGC AACGACTAAA GTGGGCAGGT 
GGAGCCAATC CAGCACTGGC CCCAGTACTG CAAGACTTTG AGATGACCAT CACTGAGAGA 
CGTGCAATTG TGGCCAGAGA AAACCAGCGC ACCAGCCAGG TCACTTTTCT GTGCTCAACT 
ATCCTTAATT TTGAAGGCCT TCGTACCCGA ACACCTGAGG CCCTGAATAT GGATGCAGCA 
CTTTTTGAGT TGTTAAAACG TTGCCAGCAA ACCTGCTCCT TTGCTGCCCA GTTTAGCAGC 
AGTGTGTCTC CACTTGAGCT TCAGCTGTTG CAAAGGCTGA GTCCAGTGCT TGACTTGCCT 
ATTGGCAGCC CCGACTGGCT GAGCTGTGCA CAAAAGCAAC TGGAGGAGCA GCTTAATGTT 
GAGCAAAGCA CTCGGGAGGA GCGGGAACAG CAGCTGGAGG TCTGTGCAGA GACTCTGCAA 
GTTCTGGTCG ATTCTGTAAA GACCATCCTG TCCCATCACA ACCGCCAGCT GGCAGATGTC 
AAGCACCTAT TGAGAGCCAT GGCAAAGGAC GAGGAGGCAG CTTTGGCAGA TGGTGAAGAG 
GTGATATATG AGGGTAGCGT GCGTCAGTTT CTGCTGGAGT ACAAAGCATG GCAAGACAAC 
ATCCAGCTGG TTTTATTCAC TGTGGTCCAG GCTAGTGGAC AGCCTCGCAG TCAGGAGCAG 
CTTGAGCTGC TGGAAGACAT TCCTGACACG CTGAAGGAAC TGTACTCTCA AAGTGAGAGT 
GTGTATAATG GCTTAGTTGG CTTCGCTTCC CCACTCGTGA CTGACCGTGA AAGTGAAAGC 
ACCAGTCCAG TGTCTGCAAT ACAGACGAGC TTTGCAGCAG CTGTCAGGTG CACTGGGTTA 
AAGACCCAGC CAGACTCGGT CTCCCAGAAT GTTCGCAAGG CTCTGCCGCG GAATCTCGGA 
ACCCCTACTG ATACCCCGCC CAGTGCTCCG TTGGTGAACA ACAAGTGTCT CAATGCCAGC 
CCAAAGAGAG CCATACGTGA CCCCAAAACT GGACGAGCTG TTCAGGAGAG AAACTCCTAT 
GCAGTCAGTG TGTGGAAGCG AGTGAAAGCC AAACTTGAGG GAAGAGATAT TGATCCGAAC 
AGAAGGATGA GTATCACAGA GCAGGTGGAC TTTGTCATCA AAGAGGCCAC AAATCTGGAC 
AACCTTGCGC AGCTATACGA AGGCTGGACT GCCTGGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg1 ( XM_029839736.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029695596.1 Takifugu rubripes SMG1 nonsense mediated mRNA decay associated PI3K related kinase (smg1), transcript variant X2, mRNA.

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CloneID	OTo70535
Clone ID Related Accession (Same CDS sequence)	XM_029839736.1
Accession Version	XM_029839736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	serine/threonine-protein kinase SMG1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGTA AATCTTCGGG GTCTCGGCTC AGCGGCGCCC ACAAACTGCA GCCACACTGG 
AATGACTGGC AGCCCAGAAC TGACAGTGCG TCACTCAGCC AGGAGGGAGA GCTCGATTCA 
TCCATATTGT ATGATATATT GCCCTCTAGC TGTCCCGATC CTCCAGTGGT GTCTAGCAAT 
GCCTTTCATG CTGCAGATAC ACACCAGGAA GAGGGTGGAT ATGAAGGCAA CAAAGTTATC 
TGTATTAATG CGTTTGGTTG GAAGTCGGTG GGGCAAGAGC TGAGGCCAAA TGACTTGACC 
ACAGATTTGC CTCGTTTTCA GCATGGAAAT CGTGCTTTAG CTTCAAAGGA CATGAAAAAA 
TCGCAGGACA GAGGAATGGG TCATTCAGAG GAGTCTCGGC TGGCCAACCT CCTGCGTCGA 
GTTTCTAGGG AGGATGATCG TGACCGCAGA CTGGCAACTC TCAGACAACT GAAGGACTTC 
ATTAGCCACA GTGAGAGTAA AGTGACACTG GTTAAACAAT TGGACACAAT CCTCAGCACC 
CTAAATGATA TTTTAAATGA GAGTAGTAAG CTACTCTGGG AGCTGCGTCA GGATGCAGCC 
ATTTGCCTTG GTCTACTCTG CACGGTTCTC AGCTATGAGG CTGAGAGCAT CTTCAAGTGG 
CTATTTCTAA AATTAACATC GAGCAATCGC GATGAAGTGA AGCTGTTGTA CCTTATGGCA 
ACATATCACG CATTGGAGGC TGCTGGAGAG AGGAAGGCCT TTTCTCACGT CATGCAGCTG 
GTGATGACCA ATTTGCAGTC CATCCTGGAG AATGTTGACA CACCAGAGCT GCTTTGTCAG 
AGTGTGAAGT GTATTCTTCA GGTGGCGCAC TGTTACCCAC ATGTGTTCAG CATCAACTTT 
AGAGATACAG TTGATATATT GGTGGGCTGG CACATAGACC ACACTCAGAA GCAGTCGGTC 
ACACAGCAAG TTTCAGGTTG GCTTCAGAGT CTGGAACAGT TCTGGGTGGC GGACCTGACA 
TTCTCCACCA CACTACTGGG ACAGTTCCTG GAGGATATGG AAGCATATGC AGAGGATCTG 
AACCATGTTG TTTTAGGGGA GATTCTCGAT GAAGATATTC CACCTCCCTC GGTGTCACTG 
CCAAAGTTAG CAGCACTGTT GAGAGTTTTC AGCACAGTGG TCCGCAGCAT TGGAGAGCGA 
TTTAGTCCCC TAAGAGGACC ACCCATTACG GAGGTCTACG TCACAGATGT TCTGAACCGT 
GTTCTGGCCT GTGTGTCGAC GGCAAAGCAG GTTCAGTTTT CTGAAGAAGT TCTCACTGCT 
GGAAACGAGT GTGTGGGTGT GTTGCTAGCT AGTGTGGAAC CCAGTGGTCA GTTGATGGAG 
GCTGTCTTGG TCTATGGGCT GGACCAGTTG GACTGCTGCC AGGCCTGCAG CTCAGACTAC 
AACCTTTCAG TGCTCATCAT CCTCACTATG ATAGTGGACC AAATCAACAC AAAGCTCCCA 
GCATCATTTG TTGAAAAACT GTTGGCTCCA GAATCCAAGT TGATGCAGTT GCGGTTTCAC 
AGAGATAAAG AGGTGATTTC AGCTGTTCAT GGTGTGTATC AGGCATTGTT AAGCCTGAAG 
AACATTCCTA CACTTGAAGC AGCATATAAA ATGGTATTGG GTGAGATAGC ATGTGCCCTC 
TCCAGCCTAA CTTTGGTGCT GAAAGCCAAT GGTATGGCTC CTGCCCCCAA TGGCTGCCTC 
TACTCAAATA TCCAACATCC TGCCTTTACT TCTCTCACCC TACCTTTGGA AAATGCAGAG 
TTCACACTGA TCTTCAACCT CAGCACCCTC ACTACCATTG GTAACACCAA GAATTCTCTG 
ATTGGGATGT GGGCCTTGTC TCCTACAGTC TTCACACTGC TCAGTCAAAA TCTTTTGTTG 
GTCCACTCTG ACTTAGCAGT GTACTACCCC ACCATCCAGT ATGCCGTACT TTACACACTG 
TACTCCCACT GCACAAGACA TGATCATTTC ATCTCATCCA GCTTATCATC TTCTCCATCA 
CTATTTGATG GTGCTGTTAT CAGCACTGTG ACCACAGCAA CAAAGAAACA CTTCAGCACA 
CTCTTAGGCC TTCTTGGAGG TCTCCTGTTG AAAGAACATC TCTACATTGA GACCAGGAGA 
CTGCTGTTGA CCTGGGCTCA GGAAATCTCG GTGCTGATGA AAAAGTCAGA CACTTATAGC 
CCTCTCTTCT CTTTGACCAC TTTCAACAAG TTTTGCAGGG GTCTCCTTGC CAATGCTCTA 
AATGAAGATC AGAGTATTTG TCTTCAGACC TGCCACTGTA TTATGGTTCT GTCTTCATCT 
TTGTCCACTG AGCTGCTGCA GAGCTGTGTG GATGTGTGTA GAGTACAGTT GGTCAACTCA 
GCAGTAAGAG TAAGGCAGGC CTATGGTAAG CTGCTCAAGA CCATTCCACT GGATGTGGCT 
TTGAGTGTCC AAAGCCAACC CAGGATGAAG GATATTTCCC TGGCTGTCCG CCACCACATG 
AGTCGAGCAC CAAGCAGCAC CTTTCATCCC CAGGACTTCA GTGACCTCAT TAGTTTCATT 
TTGTATGGGA CCTTGCACCG TGGCGGTAAG GATCCCTGGC TGGAGCGATT GTATTACAGC 
TGTCAGCGTT TTGAGAAGCT GGATGGCCGA GGCTGCGCTG ACAGTGGTAA GCCAGGTGTT 
GTACCACAAG CCCTGCTGAA AAATGAGGCT GTGCTGTGGC AGTGGGCAGC GTGGGAGGCT 
GCACAATTCA CTGTTCTGTC CAAACTCCGA ACGCCACTGG GCCGTGCTCC AGACACCTTT 
CAAACCATTG AAGGTATGAT CCGTAGCCTG GCTGCACATG CTGTCAACCC CGAACAGGAC 
GTCGGAGCGT GGGCTGGATC TGGGGGTGAT GGGGATGGTC ACCACTCCAA TCAGCTCCGT 
CTTGCTCTGT TGCTTCAGTA CCTGGAAAAC CTGGAGAAAT TAATGTACAA TGCATATGAA 
GGATGTGCCA ATGCTCTAAC TTCTCCTCCA AAGAGTATCA AAACTTTCTT TTACACAAAC 
CGTCAGACAT GCCAAGATTG GCTAACAAGG ATCCGTCTTG CTCTGATGAG AGTTGGTCTT 
CTTTCTGGCA AACCAGCAGT CACCATTCGC CATGGATTTG ACTTGCTAAC AGAGTTTAAG 
AACAGTAGTG CTCAGGGTCC AGAGGTGGAG GTCCTTCTGA CATTGTTGGC AGAAGCTCTC 
TGTGAGCTAA ATTGTCCTGA GGCCATACAG GGTTTGGCAG CATGGAGCCT TTTGACCAGT 
GGTAGAAGTC TTGGCTGGCT GTCATCAGTC GCCCTACAGG CTGAGGGAAG GTTTGAGAAG 
GCAGCTCTTG AATATCAAGA TCACCTCTCT GCAGTTACAG GGATGGACTG TAGCATCAAA 
AGCAGTGACT GCTTTTTGCC TAAATTCTCC AACAGCACAA GTAGCCCCAA ACATGTGAAC 
AATGGTGACA ATAAGAAGAC GGTGCTCCTC AAGGGAAATG AGTGTTCCTC TGAGGTCATC 
AACTTTCTGG TCAACAAAGC TAGTCAGTGT TATGTGGCGC TCTGTGACTG GGCATCAGTT 
AAAGAGTGGC AGACCACTGT CCATACCCTA AAACAAAACT CCAGCATTTC AGGAAGCATC 
AATTTGAGAA CAGATTTAAA CTACATCCAG GCCCTGAGTC GCTTTGAGGA AGGTGATCTT 
GCTGAGTGTG CAACTCAGCT TGAGCTGCTG CCTGGAGAGG ACTATAGTTT GCTCTCTTAC 
AGCAAAGACA AACTGGACCT TAAGAAACTT CTGCCTTCCA TGAGTCCGGA TCCAATTGAA 
CTGCAAAGAG CCATTGAAGT GCAGCTCCTT CGCAGTGCTG TTAGTTCCAC GCCCAAAGCC 
ACACACCAGG AGCCAAAAAG TACCCCAAGT CCCGACATGT TGCTACGATG CCTGAAGCAG 
ACTGGAAGAC TCTGTTTGGG TCCTCTCCGT TTGTCTACCC TCAATGTGGC TGACAACTTG 
CCCACCCTGC CAACCCTGCA GCTACATTGC AGTTCCATGC TGGAGAACAC ACTCATAGGA 
CAGGAGGAAT GTGTGATTCC TCTATCCAGT GAGGCTTTAA GGTCTTGTAA ACAACAGGAT 
GTCCAGCCTT TTCTTCATGC TTTCAGATAC ACTGTCTTCC AAAGACAGCA CCTTAATAAG 
GTTAAAGGCT ACTCTTCATC GACAGATGGC CATCTAACGG AGCTTTGCTT GACGGCTGTC 
AAATTTGCCA GGAAAACAGG AAACATTAGC CTAGCTTCCC GTTTGATTAG GCAGTGTGGT 
GATAGAGCCC ATGAGGAGGA GGAGAAGGGT GATTTGAGCC TCACATTCAG GCGACTCAAT 
CTGGAGGGCA CTCTTGGCGA GAAATGGGGA CCAGAGCTAC AGATAGAAAA AGCTAAAGTT 
CTTAGAGCTG CAGGCCAGTC CATGGCAGCC ATGGAGATGC TAAGTGGAGC TGCAATGTCA 
TACTGCCACA TGGGGAAGAA TGAAGGGGCT GCCTGCCGCT CACTGCTCAC ACTTTGCAAA 
TGGTTGCTGG CTGACTGGAA AGACATGAGT TCTCAACTTA AACAGGTGAT AAAGAAGTCA 
GGTGTAGTGA ACTCCAGTGC CGGCAATATA ACATCCCTCA CCAGAAACAT TTCTTCACTG 
CTGGAACTTT CCCTCAAAGA GCAGGGTATT CCCCACATCA CCGCTGAGAC CTCAGTAAAT 
GTGGGTGTTG GGGAGCCAGA CTTTGTTTTG GGCCAGCTCT ACCAGCTGTC TACATACCTG 
GCTCCAGAGA TGGCAAAGTC CTGGGCAGCT CTGGCTAGTT GGGCTTATCG CTGGGGAAGG 
AAGGTGGTGG ATAATACCAG CCATGGTGAA GGTTTGCCTC TTCTACCTGA AGAGAAAAGA 
GAGATAGAAG AGCTGCTTCC ATCAACTACC AATGACGAAG ATAAAGAGAA AATTTTCAGC 
ATTCTCGGCC AGGCCATGTG TAGACCTACT GGTATACAGG ATGAAGACAT TGCTCTCCAG 
CAGGATGATG ATGAGGATGA CATGGTGGAT GTGATCTGGA GACAACTCAC TGGCGCATGT 
CCCTGGCTTG CAAATATGGG CCAGGGTGTC TCAGATGGTC TGATTCAGGT TTGGAGACAT 
GTGGTGGATC GCATCTTTGG CCTCTACAGG GTCTCATGCC AGGCCTATTT TACTTTTTTG 
AAGCTCAATG TTGGACAGGT CCCTTTAGAT GAAGATGATC CAAAAGCCTT GCTGTCATTT 
CAAAACAACA AACAGAGCAA TGATGATGTG ATTGTCATGG CAACCCTGCG GCTCCTTCGA 
TTGCTGGTGA AGCATGCTGG TGAATTGAGA GAAGTACTGG AGTTGGGTTT AGCCTCGACG 
CCTACAGGAC CCTGGAGAGG GATCATCCCC CAGCTTTTTT CCCGTCTCAA TCATCCAGAG 
GCATATATCC GGCAAAGCAT ATGCAGTTTG CTGTGTCGAG TGGCCCAAGA CTCCCCGCAT 
CTCATACTCT ACCCTGCCAT TGTGGGCTCA CTATCCTTTG GAACAGAAGC CCAGAATGCA 
GGAAATCAAC TACCTTCAGC ACTTCCCACG TTTTTGGGAA GTATGCAGGG AGAGGGCATT 
GTAGGAGAGG AGCAGGCCGA TGGCAACGGA CCAGATGGAG CGCCAAAAGC AGAACTAGCA 
AACTTGTACT CCAGTCAAGA CCAAGCCATG ATGCAGGATT GTTACAGCAA GATAGTGGAA 
AAACTGTCCA TTGCAAATCC TGCCATGGTG CTTCAGGTCC AACAGCTGGT GGGTGAACTA 
CGCAGAGTTA CCTTACTCTG GGATGAATTG TGGCTCGGTG TGTTGCAACA GCAACATATG 
CATGTCCTTC GTAGGATCCA GCAGCTCGAG GATGAGGTTA AGAGGGTTCA GAATAACGCC 
ACGCTGCGCA GGGATGAAAA GATTGCTATC ATGCGGGAAA AACACTCTGC TTTGATGCGT 
CCTGTGGTTT TTGCTCTGGA TCACGTTTGC AGTATCACTG CAGCCCCTGC AGAGACACCA 
CATGAAGTGT GGTTCCAGCA GACTTACGGG AGTGATATCA TATTTGCCCT TGAACGACTG 
AGAAACCCTA ATAACCCCGC CAACCCTGCC AGCAGCTGGC TTCCCTTTAA ACAGATCATG 
TTGAGCCTGC AGCAGCGTGC CCAAAAGCGA GCTAGCTACC TGCTTCACCT GGAGGAGATC 
AGTCCTCATC TTGTGTCAAT GACGAAGACA GAAATGGCCT TGCCTGGTGA GGTGTCAGCA 
TCAGACGCTA TCACCATACA AAGCATAGGC AATACCATTA CCATTCTACC CACTAAGACC 
AAGCCCAAAA AGCTGTTCTT CATGGGCTCA GATGGACGAC ATTACCCATA CCTCTTTAAA 
GGTCTGGAGG ATTTACATTT GGATGAGCGC ATCATGCAGT TTCTGTCCAT TGTCAATACC 
ATGTTCACCA AGATTAACCA GCAAGAGCAG CCTCAATTCC ATGCCTGCCA CTACTCTGTC 
ACCCCGCTTG GAACCAGGTC AGGGCTTATT CAGTGGGTGG ATGGAGCCAC ACCACTGTTT 
GGCCTCTACA AACGCTGGCA GCAAAGGGAG GCTGTGCTCC AGACCCAGAA GGGCCAAGAT 
TCATTGCAGC AACAGCTGCC AATCCCTCCA GTACCTCGCC CAAGTGAACT GTACTACAGC 
CGCATTGGGC CAGCTCTGAA GGCAGTGGGG CTCAGCCTGG ACGTGACGCG ACGAGATTGG 
CCTCTAAGTG TCATGAAAGA GGTGCTAAAA GAGTTGATGG AGGCTACACC TTCCAACTTG 
CTTGCTAAAG AGCTGTGGTG TTCCTGCACC ACGCCAAGTG AGTGGTGGAG AGTAACTCAG 
TCTTATGCCA GGTCCACTGC TGTCATGTCG ATGGTTGGCT ATATCATTGG CCTTGGTGAC 
CGTCATCTAG ACAATGTGTT GATTGACATG ACCACAGGAG AGGTGGTGCA CATTGACTAC 
AATGTATGCT TTGAGAAAGG GAAGAGTCTA CGAGTGCCAG AGAAAGTCCC ATTCAGGATG 
ACCCACAACA TTGAGACTGC TCTGGGTGTC ACCGGTGTGG AGGGCATCTT CAGGCTGTCC 
TGTGAACAGG TTCTTCAGAT CATGCGTCGT GGCAGAGAGA CCCTGTTGAC CCTGCTGGAG 
GCCTTTGTCT ACGATCCTCT TGTGGACTGG ACTGCTGGTG GGGAAGTGGG TTTTGCTGGC 
GCTGTTTATG GAGGAGGAGG CCAACAAGCT GAAAACAAGC AGAGCAAAAG GGAGATGGAA 
AGGGACATTA CACGCTCTCT TTTCTCATCC AGGGTAGCTG AGATTAAGGT GAATTGGTTT 
AAAAATCGTG ATGAAATGCT GGCAGTGTTG CCACAAGTGG AAGAGGCTGT GGAAGAGTAC 
CTGGTTCTGC AGGAGCTGCT CTGCCAGGGT GGGAAACTGC AGGACAAACT TCAAGAAGAG 
ATAACCTTCC TAGAAGGAGC AGAGAACCAT CCAGACCATC TTATTCTTAC CCTGGAGCAA 
AGGTACTCTG AACACACTCG GCTTCAGTCA CAGCAGCGAA CGATGCAGGA GGCCATTCAA 
AGCAAACTCA CTGACCTTGA CCAGTGGATT TCCCAGTACC AGGCAGCTTT CTCCAACCTG 
GAAGCTGCCC AGCTGGCCAC TTTGCTTCAA GACATCAGTT GCCCCATAGA TTTGGGTCCT 
CCCAGCTATG TACCTGCTAC AGCCTTCCTA CAGAATGCAG GCCAGGCCCA TTTGATCACC 
CAGTGTGAGA ACTTGGAAGC AGAATTGAGC ACTTTGCTGC AGCAGCGACG TGGGGCCCTT 
CGTGGTTGCC TAGAGCAGTT GCACAGCTAC GCAACAGTAG CATTACTGTA CCCAAGAGGT 
ACTTTGCGGC TCCATCGAGT CCAGCAGTGG AAGGCCTGGT TAGAGGAATT GTTGACAGAC 
ATGACGGTGG AGCAGTGCCA GCATATCTAC CGACAATATG AGATACTCTT TGCACCTCAG 
CCTTCTGCAG CTATCTGTCA GTTTTTGTCA AGTGTGGAGT TGACTTTGCA ACATCAGATG 
GCAGAAACCA ATGATCGTGT GATGCATCAG GCAGAGCGGC TCAAGGCCGA GGGAACGACT 
GTGGCAGTGT GCGAAGATCA AGTACAGGAG ATTGACCACT GTATCACAGG TTTTCTACAT 
GAGAACAGAG AGTATGGTTC TCTGGGATTG GCAGGAATCA TCACCTCTCT CCTGTGTACA 
CTGTGCAGAA GAAATCTGGT TATGGAAACT GCTGCAGCCA ATGCTGGAGA ACAGCTTATT 
GACTTAAAGT CTCGAGATGG GACATGGTTC CTGGAGGAGC TCTGCAGTAT GTTTGGAAAT 
GTCAGTGGCC TGACAACACT TCTACAACGC TGCCAACTTG TACAACATGA TCTGGAGCTG 
CCTGCCCTCT CAGACACCAT CCAAGCTGTT TATCTGGCCA ATGTTGTATA TACCTGCTTA 
CAAGAACTAA ACACCAATTT TCGACAGATC ATCTTTCCAG AAGCTTTGTG TTGTATGATA 
AAAGGAGAGC CCACTCTCGA GTCCATGTTG GTGGAGATAG AGCAGCTGGT GGAGCAGAGC 
TCTGATGGTC TTGGACTGCA GGGATTGGTT GACAGTCTGC AATCTACTAT GCGGTTCGAC 
CAAGATGGAC ACAACCATTT GCTTCACATT ACAAGTTTAC TGCGTGCTCA GTACTCTGGG 
CTGATCCAGC CTCGGAACAC AGAAGGACCA GGTCACGACA CACCAAAGAT GTCCCCAGGA 
CAAATGTTGT TGGTGGCGTT TGATGGAATG TTCACCCAGC TAGAGAATGC ATTTATGCAG 
CTCACAGAGA AGCTGAGTTG TCTCGAGGTG CCTTCAGCCT GGCAAAAAAT AGATTCTTTG 
AGGGAAGCTC GGACCGCACA GCTCTATTTC TTTGAGAATC GGAGTCAGAG ACAACTCATG 
GAAGAGGTAT TCTTCCTCAA AAGACTGCAG ACCATTAGGG ATTTCTTCAA GCTTTGTGCC 
ACATTTGCCC AAACTATCTC TGGTACCTGT CCTTCAACTG AGGACCTCTT AACAAATGGG 
CCAGTAGGAT CGGGGAAGTC ATTCTACCAG CTGCCCACTG CATCAGACTT GGGTGTGCCA 
GTGGTCAGTG AAGAAAAGAT GACCCATCCG ATTAAGGCCT TCACAGCTGA GTTTGTCCGA 
CAGATGCTCA TGGGTCTGCC CAGCCAAACA CTAAGCCTAG TTCTGTGCAG CGCCCTTTCT 
TCTCTTGGGC TGGATATTGT TTCCCTGGTG GAGGCCAAAG ACTTTGGTGT TGAGAGAAAG 
GTTTCTCTGG ATGAACTTTG TAAGACGGCA GTGGAGCAGA GCCTAGCTTC AGGCCGGGTT 
TCCCAGCTGT TGTTGAACAG AGCCACTGTA TTGGCCTCCT CCTATGATAC AGCTTGGAAG 
AAACTAGACC TTCTCAGAAG ACTGGATGCC CACCTGGAGG CTGCCAAGGC CAGTTTGCAG 
CGAAGCCAGC TGCATATCGC CATGTTTCAG TGGCATCATG AGGATATTCT GGGCCCCAGA 
AATCAGCCTC TAGGTGTAAG CATTCCACCT CGTACCATCA TTCTTAGCAA CATGAAGAAA 
AAACTTTACA AGCTGAGTCA GGATGAGGGA GCCATAGTTG CTGTTCAGGA GAAGCTGGCG 
TCACTGGAGG CTAGCATTGA GCAACGACTA AAGTGGGCAG GTGGAGCCAA TCCAGCACTG 
GCCCCAGTAC TGCAAGACTT TGAGATGACC ATCACTGAGA GACGTGCAAT TGTGGCCAGA 
GAAAACCAGC GCACCAGCCA GGTCACTTTT CTGTGCTCAA CTATCCTTAA TTTTGAAGGC 
CTTCGTACCC GAACACCTGA GGCCCTGAAT ATGGATGCAG CACTTTTTGA GTTGTTAAAA 
CGTTGCCAGC AAACCTGCTC CTTTGCTGCC CAGTTTAGCA GCAGTGTGTC TCCACTTGAG 
CTTCAGCTGT TGCAAAGGCT GAGTCCAGTG CTTGACTTGC CTATTGGCAG CCCCGACTGG 
CTGAGCTGTG CACAAAAGCA ACTGGAGGAG CAGCTTAATG TTGAGCAAAG CACTCGGGAG 
GAGCGGGAAC AGCAGCTGGA GGTCTGTGCA GAGACTCTGC AAGTTCTGGT CGATTCTGTA 
AAGACCATCC TGTCCCATCA CAACCGCCAG CTGGCAGATG TCAAGCACCT ATTGAGAGCC 
ATGGCAAAGG ACGAGGAGGC AGCTTTGGCA GATGGTGAAG AGGTGATATA TGAGGGTAGC 
GTGCGTCAGT TTCTGCTGGA GTACAAAGCA TGGCAAGACA ACATCCAGCT GGTTTTATTC 
ACTGTGGTCC AGGCTAGTGG ACAGCCTCGC AGTCAGGAGC AGCTTGAGCT GCTGGAAGAC 
ATTCCTGACA CGCTGAAGGA ACTGTACTCT CAAAGTGAGA GTGTGTATAA TGGCTTAGTT 
GGCTTCGCTT CCCCACTCGT GACTGACCGT GAAAGTGAAA GCACCAGTCC AGTGTCTGCA 
ATACAGACGA GCTTTGCAGC AGCTGTCAGG TGCACTGGGT TAAAGACCCA GCCAGACTCG 
GTCTCCCAGA ATGTTCGCAA GGCTCTGCCG CGGAATCTCG GAACCCCTAC TGATACCCCG 
CCCAGTGCTC CGTTGGTGAA CAACAAGTGT CTCAATGCCA GCCCAAAGAG AGCCATACGT 
GACCCCAAAA CTGGACGAGC TGTTCAGGAG AGAAACTCCT ATGCAGTCAG TGTGTGGAAG 
CGAGTGAAAG CCAAACTTGA GGGAAGAGAT ATTGATCCGA ACAGAAGGAT GAGTATCACA 
GAGCAGGTGG ACTTTGTCAT CAAAGAGGCC ACAAATCTGG ACAACCTTGC GCAGCTATAC 
GAAGGCTGGA CTGCCTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029695596.1
CDS	142..11085
Translation	MSRKSSGSRLSGAHKLQPHWNDWQPRTDSASLSQEGELDSSILYDILPSSCPDPPVVSSNAFHAADTHQEEGGYEGNKVICINAFGWKSVGQELRPNDLTTDLPRFQHGNRALASKDMKKSQDRGMGHSEESRLANLLRRVSREDDRDRRLATLRQLKDFISHSESKVTLVKQLDTILSTLNDILNESSKLLWELRQDAAICLGLLCTVLSYEAESIFKWLFLKLTSSNRDEVKLLYLMATYHALEAAGERKAFSHVMQLVMTNLQSILENVDTPELLCQSVKCILQVAHCYPHVFSINFRDTVDILVGWHIDHTQKQSVTQQVSGWLQSLEQFWVADLTFSTTLLGQFLEDMEAYAEDLNHVVLGEILDEDIPPPSVSLPKLAALLRVFSTVVRSIGERFSPLRGPPITEVYVTDVLNRVLACVSTAKQVQFSEEVLTAGNECVGVLLASVEPSGQLMEAVLVYGLDQLDCCQACSSDYNLSVLIILTMIVDQINTKLPASFVEKLLAPESKLMQLRFHRDKEVISAVHGVYQALLSLKNIPTLEAAYKMVLGEIACALSSLTLVLKANGMAPAPNGCLYSNIQHPAFTSLTLPLENAEFTLIFNLSTLTTIGNTKNSLIGMWALSPTVFTLLSQNLLLVHSDLAVYYPTIQYAVLYTLYSHCTRHDHFISSSLSSSPSLFDGAVISTVTTATKKHFSTLLGLLGGLLLKEHLYIETRRLLLTWAQEISVLMKKSDTYSPLFSLTTFNKFCRGLLANALNEDQSICLQTCHCIMVLSSSLSTELLQSCVDVCRVQLVNSAVRVRQAYGKLLKTIPLDVALSVQSQPRMKDISLAVRHHMSRAPSSTFHPQDFSDLISFILYGTLHRGGKDPWLERLYYSCQRFEKLDGRGCADSGKPGVVPQALLKNEAVLWQWAAWEAAQFTVLSKLRTPLGRAPDTFQTIEGMIRSLAAHAVNPEQDVGAWAGSGGDGDGHHSNQLRLALLLQYLENLEKLMYNAYEGCANALTSPPKSIKTFFYTNRQTCQDWLTRIRLALMRVGLLSGKPAVTIRHGFDLLTEFKNSSAQGPEVEVLLTLLAEALCELNCPEAIQGLAAWSLLTSGRSLGWLSSVALQAEGRFEKAALEYQDHLSAVTGMDCSIKSSDCFLPKFSNSTSSPKHVNNGDNKKTVLLKGNECSSEVINFLVNKASQCYVALCDWASVKEWQTTVHTLKQNSSISGSINLRTDLNYIQALSRFEEGDLAECATQLELLPGEDYSLLSYSKDKLDLKKLLPSMSPDPIELQRAIEVQLLRSAVSSTPKATHQEPKSTPSPDMLLRCLKQTGRLCLGPLRLSTLNVADNLPTLPTLQLHCSSMLENTLIGQEECVIPLSSEALRSCKQQDVQPFLHAFRYTVFQRQHLNKVKGYSSSTDGHLTELCLTAVKFARKTGNISLASRLIRQCGDRAHEEEEKGDLSLTFRRLNLEGTLGEKWGPELQIEKAKVLRAAGQSMAAMEMLSGAAMSYCHMGKNEGAACRSLLTLCKWLLADWKDMSSQLKQVIKKSGVVNSSAGNITSLTRNISSLLELSLKEQGIPHITAETSVNVGVGEPDFVLGQLYQLSTYLAPEMAKSWAALASWAYRWGRKVVDNTSHGEGLPLLPEEKREIEELLPSTTNDEDKEKIFSILGQAMCRPTGIQDEDIALQQDDDEDDMVDVIWRQLTGACPWLANMGQGVSDGLIQVWRHVVDRIFGLYRVSCQAYFTFLKLNVGQVPLDEDDPKALLSFQNNKQSNDDVIVMATLRLLRLLVKHAGELREVLELGLASTPTGPWRGIIPQLFSRLNHPEAYIRQSICSLLCRVAQDSPHLILYPAIVGSLSFGTEAQNAGNQLPSALPTFLGSMQGEGIVGEEQADGNGPDGAPKAELANLYSSQDQAMMQDCYSKIVEKLSIANPAMVLQVQQLVGELRRVTLLWDELWLGVLQQQHMHVLRRIQQLEDEVKRVQNNATLRRDEKIAIMREKHSALMRPVVFALDHVCSITAAPAETPHEVWFQQTYGSDIIFALERLRNPNNPANPASSWLPFKQIMLSLQQRAQKRASYLLHLEEISPHLVSMTKTEMALPGEVSASDAITIQSIGNTITILPTKTKPKKLFFMGSDGRHYPYLFKGLEDLHLDERIMQFLSIVNTMFTKINQQEQPQFHACHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAVLQTQKGQDSLQQQLPIPPVPRPSELYYSRIGPALKAVGLSLDVTRRDWPLSVMKEVLKELMEATPSNLLAKELWCSCTTPSEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTHNIETALGVTGVEGIFRLSCEQVLQIMRRGRETLLTLLEAFVYDPLVDWTAGGEVGFAGAVYGGGGQQAENKQSKREMERDITRSLFSSRVAEIKVNWFKNRDEMLAVLPQVEEAVEEYLVLQELLCQGGKLQDKLQEEITFLEGAENHPDHLILTLEQRYSEHTRLQSQQRTMQEAIQSKLTDLDQWISQYQAAFSNLEAAQLATLLQDISCPIDLGPPSYVPATAFLQNAGQAHLITQCENLEAELSTLLQQRRGALRGCLEQLHSYATVALLYPRGTLRLHRVQQWKAWLEELLTDMTVEQCQHIYRQYEILFAPQPSAAICQFLSSVELTLQHQMAETNDRVMHQAERLKAEGTTVAVCEDQVQEIDHCITGFLHENREYGSLGLAGIITSLLCTLCRRNLVMETAAANAGEQLIDLKSRDGTWFLEELCSMFGNVSGLTTLLQRCQLVQHDLELPALSDTIQAVYLANVVYTCLQELNTNFRQIIFPEALCCMIKGEPTLESMLVEIEQLVEQSSDGLGLQGLVDSLQSTMRFDQDGHNHLLHITSLLRAQYSGLIQPRNTEGPGHDTPKMSPGQMLLVAFDGMFTQLENAFMQLTEKLSCLEVPSAWQKIDSLREARTAQLYFFENRSQRQLMEEVFFLKRLQTIRDFFKLCATFAQTISGTCPSTEDLLTNGPVGSGKSFYQLPTASDLGVPVVSEEKMTHPIKAFTAEFVRQMLMGLPSQTLSLVLCSALSSLGLDIVSLVEAKDFGVERKVSLDELCKTAVEQSLASGRVSQLLLNRATVLASSYDTAWKKLDLLRRLDAHLEAAKASLQRSQLHIAMFQWHHEDILGPRNQPLGVSIPPRTIILSNMKKKLYKLSQDEGAIVAVQEKLASLEASIEQRLKWAGGANPALAPVLQDFEMTITERRAIVARENQRTSQVTFLCSTILNFEGLRTRTPEALNMDAALFELLKRCQQTCSFAAQFSSSVSPLELQLLQRLSPVLDLPIGSPDWLSCAQKQLEEQLNVEQSTREEREQQLEVCAETLQVLVDSVKTILSHHNRQLADVKHLLRAMAKDEEAALADGEEVIYEGSVRQFLLEYKAWQDNIQLVLFTVVQASGQPRSQEQLELLEDIPDTLKELYSQSESVYNGLVGFASPLVTDRESESTSPVSAIQTSFAAAVRCTGLKTQPDSVSQNVRKALPRNLGTPTDTPPSAPLVNNKCLNASPKRAIRDPKTGRAVQERNSYAVSVWKRVKAKLEGRDIDPNRRMSITEQVDFVIKEATNLDNLAQLYEGWTAWV

Target ORF information:

RefSeq Version	XM_029839736.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes SMG1 nonsense mediated mRNA decay associated PI3K related kinase (smg1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029839736.1

ORF Insert Sequence:

ATGAGCCGTA AATCTTCGGG GTCTCGGCTC AGCGGCGCCC ACAAACTGCA GCCACACTGG 
AATGACTGGC AGCCCAGAAC TGACAGTGCG TCACTCAGCC AGGAGGGAGA GCTCGATTCA 
TCCATATTGT ATGATATATT GCCCTCTAGC TGTCCCGATC CTCCAGTGGT GTCTAGCAAT 
GCCTTTCATG CTGCAGATAC ACACCAGGAA GAGGGTGGAT ATGAAGGCAA CAAAGTTATC 
TGTATTAATG CGTTTGGTTG GAAGTCGGTG GGGCAAGAGC TGAGGCCAAA TGACTTGACC 
ACAGATTTGC CTCGTTTTCA GCATGGAAAT CGTGCTTTAG CTTCAAAGGA CATGAAAAAA 
TCGCAGGACA GAGGAATGGG TCATTCAGAG GAGTCTCGGC TGGCCAACCT CCTGCGTCGA 
GTTTCTAGGG AGGATGATCG TGACCGCAGA CTGGCAACTC TCAGACAACT GAAGGACTTC 
ATTAGCCACA GTGAGAGTAA AGTGACACTG GTTAAACAAT TGGACACAAT CCTCAGCACC 
CTAAATGATA TTTTAAATGA GAGTAGTAAG CTACTCTGGG AGCTGCGTCA GGATGCAGCC 
ATTTGCCTTG GTCTACTCTG CACGGTTCTC AGCTATGAGG CTGAGAGCAT CTTCAAGTGG 
CTATTTCTAA AATTAACATC GAGCAATCGC GATGAAGTGA AGCTGTTGTA CCTTATGGCA 
ACATATCACG CATTGGAGGC TGCTGGAGAG AGGAAGGCCT TTTCTCACGT CATGCAGCTG 
GTGATGACCA ATTTGCAGTC CATCCTGGAG AATGTTGACA CACCAGAGCT GCTTTGTCAG 
AGTGTGAAGT GTATTCTTCA GGTGGCGCAC TGTTACCCAC ATGTGTTCAG CATCAACTTT 
AGAGATACAG TTGATATATT GGTGGGCTGG CACATAGACC ACACTCAGAA GCAGTCGGTC 
ACACAGCAAG TTTCAGGTTG GCTTCAGAGT CTGGAACAGT TCTGGGTGGC GGACCTGACA 
TTCTCCACCA CACTACTGGG ACAGTTCCTG GAGGATATGG AAGCATATGC AGAGGATCTG 
AACCATGTTG TTTTAGGGGA GATTCTCGAT GAAGATATTC CACCTCCCTC GGTGTCACTG 
CCAAAGTTAG CAGCACTGTT GAGAGTTTTC AGCACAGTGG TCCGCAGCAT TGGAGAGCGA 
TTTAGTCCCC TAAGAGGACC ACCCATTACG GAGGTCTACG TCACAGATGT TCTGAACCGT 
GTTCTGGCCT GTGTGTCGAC GGCAAAGCAG GTTCAGTTTT CTGAAGAAGT TCTCACTGCT 
GGAAACGAGT GTGTGGGTGT GTTGCTAGCT AGTGTGGAAC CCAGTGGTCA GTTGATGGAG 
GCTGTCTTGG TCTATGGGCT GGACCAGTTG GACTGCTGCC AGGCCTGCAG CTCAGACTAC 
AACCTTTCAG TGCTCATCAT CCTCACTATG ATAGTGGACC AAATCAACAC AAAGCTCCCA 
GCATCATTTG TTGAAAAACT GTTGGCTCCA GAATCCAAGT TGATGCAGTT GCGGTTTCAC 
AGAGATAAAG AGGTGATTTC AGCTGTTCAT GGTGTGTATC AGGCATTGTT AAGCCTGAAG 
AACATTCCTA CACTTGAAGC AGCATATAAA ATGGTATTGG GTGAGATAGC ATGTGCCCTC 
TCCAGCCTAA CTTTGGTGCT GAAAGCCAAT GGTATGGCTC CTGCCCCCAA TGGCTGCCTC 
TACTCAAATA TCCAACATCC TGCCTTTACT TCTCTCACCC TACCTTTGGA AAATGCAGAG 
TTCACACTGA TCTTCAACCT CAGCACCCTC ACTACCATTG GTAACACCAA GAATTCTCTG 
ATTGGGATGT GGGCCTTGTC TCCTACAGTC TTCACACTGC TCAGTCAAAA TCTTTTGTTG 
GTCCACTCTG ACTTAGCAGT GTACTACCCC ACCATCCAGT ATGCCGTACT TTACACACTG 
TACTCCCACT GCACAAGACA TGATCATTTC ATCTCATCCA GCTTATCATC TTCTCCATCA 
CTATTTGATG GTGCTGTTAT CAGCACTGTG ACCACAGCAA CAAAGAAACA CTTCAGCACA 
CTCTTAGGCC TTCTTGGAGG TCTCCTGTTG AAAGAACATC TCTACATTGA GACCAGGAGA 
CTGCTGTTGA CCTGGGCTCA GGAAATCTCG GTGCTGATGA AAAAGTCAGA CACTTATAGC 
CCTCTCTTCT CTTTGACCAC TTTCAACAAG TTTTGCAGGG GTCTCCTTGC CAATGCTCTA 
AATGAAGATC AGAGTATTTG TCTTCAGACC TGCCACTGTA TTATGGTTCT GTCTTCATCT 
TTGTCCACTG AGCTGCTGCA GAGCTGTGTG GATGTGTGTA GAGTACAGTT GGTCAACTCA 
GCAGTAAGAG TAAGGCAGGC CTATGGTAAG CTGCTCAAGA CCATTCCACT GGATGTGGCT 
TTGAGTGTCC AAAGCCAACC CAGGATGAAG GATATTTCCC TGGCTGTCCG CCACCACATG 
AGTCGAGCAC CAAGCAGCAC CTTTCATCCC CAGGACTTCA GTGACCTCAT TAGTTTCATT 
TTGTATGGGA CCTTGCACCG TGGCGGTAAG GATCCCTGGC TGGAGCGATT GTATTACAGC 
TGTCAGCGTT TTGAGAAGCT GGATGGCCGA GGCTGCGCTG ACAGTGGTAA GCCAGGTGTT 
GTACCACAAG CCCTGCTGAA AAATGAGGCT GTGCTGTGGC AGTGGGCAGC GTGGGAGGCT 
GCACAATTCA CTGTTCTGTC CAAACTCCGA ACGCCACTGG GCCGTGCTCC AGACACCTTT 
CAAACCATTG AAGGTATGAT CCGTAGCCTG GCTGCACATG CTGTCAACCC CGAACAGGAC 
GTCGGAGCGT GGGCTGGATC TGGGGGTGAT GGGGATGGTC ACCACTCCAA TCAGCTCCGT 
CTTGCTCTGT TGCTTCAGTA CCTGGAAAAC CTGGAGAAAT TAATGTACAA TGCATATGAA 
GGATGTGCCA ATGCTCTAAC TTCTCCTCCA AAGAGTATCA AAACTTTCTT TTACACAAAC 
CGTCAGACAT GCCAAGATTG GCTAACAAGG ATCCGTCTTG CTCTGATGAG AGTTGGTCTT 
CTTTCTGGCA AACCAGCAGT CACCATTCGC CATGGATTTG ACTTGCTAAC AGAGTTTAAG 
AACAGTAGTG CTCAGGGTCC AGAGGTGGAG GTCCTTCTGA CATTGTTGGC AGAAGCTCTC 
TGTGAGCTAA ATTGTCCTGA GGCCATACAG GGTTTGGCAG CATGGAGCCT TTTGACCAGT 
GGTAGAAGTC TTGGCTGGCT GTCATCAGTC GCCCTACAGG CTGAGGGAAG GTTTGAGAAG 
GCAGCTCTTG AATATCAAGA TCACCTCTCT GCAGTTACAG GGATGGACTG TAGCATCAAA 
AGCAGTGACT GCTTTTTGCC TAAATTCTCC AACAGCACAA GTAGCCCCAA ACATGTGAAC 
AATGGTGACA ATAAGAAGAC GGTGCTCCTC AAGGGAAATG AGTGTTCCTC TGAGGTCATC 
AACTTTCTGG TCAACAAAGC TAGTCAGTGT TATGTGGCGC TCTGTGACTG GGCATCAGTT 
AAAGAGTGGC AGACCACTGT CCATACCCTA AAACAAAACT CCAGCATTTC AGGAAGCATC 
AATTTGAGAA CAGATTTAAA CTACATCCAG GCCCTGAGTC GCTTTGAGGA AGGTGATCTT 
GCTGAGTGTG CAACTCAGCT TGAGCTGCTG CCTGGAGAGG ACTATAGTTT GCTCTCTTAC 
AGCAAAGACA AACTGGACCT TAAGAAACTT CTGCCTTCCA TGAGTCCGGA TCCAATTGAA 
CTGCAAAGAG CCATTGAAGT GCAGCTCCTT CGCAGTGCTG TTAGTTCCAC GCCCAAAGCC 
ACACACCAGG AGCCAAAAAG TACCCCAAGT CCCGACATGT TGCTACGATG CCTGAAGCAG 
ACTGGAAGAC TCTGTTTGGG TCCTCTCCGT TTGTCTACCC TCAATGTGGC TGACAACTTG 
CCCACCCTGC CAACCCTGCA GCTACATTGC AGTTCCATGC TGGAGAACAC ACTCATAGGA 
CAGGAGGAAT GTGTGATTCC TCTATCCAGT GAGGCTTTAA GGTCTTGTAA ACAACAGGAT 
GTCCAGCCTT TTCTTCATGC TTTCAGATAC ACTGTCTTCC AAAGACAGCA CCTTAATAAG 
GTTAAAGGCT ACTCTTCATC GACAGATGGC CATCTAACGG AGCTTTGCTT GACGGCTGTC 
AAATTTGCCA GGAAAACAGG AAACATTAGC CTAGCTTCCC GTTTGATTAG GCAGTGTGGT 
GATAGAGCCC ATGAGGAGGA GGAGAAGGGT GATTTGAGCC TCACATTCAG GCGACTCAAT 
CTGGAGGGCA CTCTTGGCGA GAAATGGGGA CCAGAGCTAC AGATAGAAAA AGCTAAAGTT 
CTTAGAGCTG CAGGCCAGTC CATGGCAGCC ATGGAGATGC TAAGTGGAGC TGCAATGTCA 
TACTGCCACA TGGGGAAGAA TGAAGGGGCT GCCTGCCGCT CACTGCTCAC ACTTTGCAAA 
TGGTTGCTGG CTGACTGGAA AGACATGAGT TCTCAACTTA AACAGGTGAT AAAGAAGTCA 
GGTGTAGTGA ACTCCAGTGC CGGCAATATA ACATCCCTCA CCAGAAACAT TTCTTCACTG 
CTGGAACTTT CCCTCAAAGA GCAGGGTATT CCCCACATCA CCGCTGAGAC CTCAGTAAAT 
GTGGGTGTTG GGGAGCCAGA CTTTGTTTTG GGCCAGCTCT ACCAGCTGTC TACATACCTG 
GCTCCAGAGA TGGCAAAGTC CTGGGCAGCT CTGGCTAGTT GGGCTTATCG CTGGGGAAGG 
AAGGTGGTGG ATAATACCAG CCATGGTGAA GGTTTGCCTC TTCTACCTGA AGAGAAAAGA 
GAGATAGAAG AGCTGCTTCC ATCAACTACC AATGACGAAG ATAAAGAGAA AATTTTCAGC 
ATTCTCGGCC AGGCCATGTG TAGACCTACT GGTATACAGG ATGAAGACAT TGCTCTCCAG 
CAGGATGATG ATGAGGATGA CATGGTGGAT GTGATCTGGA GACAACTCAC TGGCGCATGT 
CCCTGGCTTG CAAATATGGG CCAGGGTGTC TCAGATGGTC TGATTCAGGT TTGGAGACAT 
GTGGTGGATC GCATCTTTGG CCTCTACAGG GTCTCATGCC AGGCCTATTT TACTTTTTTG 
AAGCTCAATG TTGGACAGGT CCCTTTAGAT GAAGATGATC CAAAAGCCTT GCTGTCATTT 
CAAAACAACA AACAGAGCAA TGATGATGTG ATTGTCATGG CAACCCTGCG GCTCCTTCGA 
TTGCTGGTGA AGCATGCTGG TGAATTGAGA GAAGTACTGG AGTTGGGTTT AGCCTCGACG 
CCTACAGGAC CCTGGAGAGG GATCATCCCC CAGCTTTTTT CCCGTCTCAA TCATCCAGAG 
GCATATATCC GGCAAAGCAT ATGCAGTTTG CTGTGTCGAG TGGCCCAAGA CTCCCCGCAT 
CTCATACTCT ACCCTGCCAT TGTGGGCTCA CTATCCTTTG GAACAGAAGC CCAGAATGCA 
GGAAATCAAC TACCTTCAGC ACTTCCCACG TTTTTGGGAA GTATGCAGGG AGAGGGCATT 
GTAGGAGAGG AGCAGGCCGA TGGCAACGGA CCAGATGGAG CGCCAAAAGC AGAACTAGCA 
AACTTGTACT CCAGTCAAGA CCAAGCCATG ATGCAGGATT GTTACAGCAA GATAGTGGAA 
AAACTGTCCA TTGCAAATCC TGCCATGGTG CTTCAGGTCC AACAGCTGGT GGGTGAACTA 
CGCAGAGTTA CCTTACTCTG GGATGAATTG TGGCTCGGTG TGTTGCAACA GCAACATATG 
CATGTCCTTC GTAGGATCCA GCAGCTCGAG GATGAGGTTA AGAGGGTTCA GAATAACGCC 
ACGCTGCGCA GGGATGAAAA GATTGCTATC ATGCGGGAAA AACACTCTGC TTTGATGCGT 
CCTGTGGTTT TTGCTCTGGA TCACGTTTGC AGTATCACTG CAGCCCCTGC AGAGACACCA 
CATGAAGTGT GGTTCCAGCA GACTTACGGG AGTGATATCA TATTTGCCCT TGAACGACTG 
AGAAACCCTA ATAACCCCGC CAACCCTGCC AGCAGCTGGC TTCCCTTTAA ACAGATCATG 
TTGAGCCTGC AGCAGCGTGC CCAAAAGCGA GCTAGCTACC TGCTTCACCT GGAGGAGATC 
AGTCCTCATC TTGTGTCAAT GACGAAGACA GAAATGGCCT TGCCTGGTGA GGTGTCAGCA 
TCAGACGCTA TCACCATACA AAGCATAGGC AATACCATTA CCATTCTACC CACTAAGACC 
AAGCCCAAAA AGCTGTTCTT CATGGGCTCA GATGGACGAC ATTACCCATA CCTCTTTAAA 
GGTCTGGAGG ATTTACATTT GGATGAGCGC ATCATGCAGT TTCTGTCCAT TGTCAATACC 
ATGTTCACCA AGATTAACCA GCAAGAGCAG CCTCAATTCC ATGCCTGCCA CTACTCTGTC 
ACCCCGCTTG GAACCAGGTC AGGGCTTATT CAGTGGGTGG ATGGAGCCAC ACCACTGTTT 
GGCCTCTACA AACGCTGGCA GCAAAGGGAG GCTGTGCTCC AGACCCAGAA GGGCCAAGAT 
TCATTGCAGC AACAGCTGCC AATCCCTCCA GTACCTCGCC CAAGTGAACT GTACTACAGC 
CGCATTGGGC CAGCTCTGAA GGCAGTGGGG CTCAGCCTGG ACGTGACGCG ACGAGATTGG 
CCTCTAAGTG TCATGAAAGA GGTGCTAAAA GAGTTGATGG AGGCTACACC TTCCAACTTG 
CTTGCTAAAG AGCTGTGGTG TTCCTGCACC ACGCCAAGTG AGTGGTGGAG AGTAACTCAG 
TCTTATGCCA GGTCCACTGC TGTCATGTCG ATGGTTGGCT ATATCATTGG CCTTGGTGAC 
CGTCATCTAG ACAATGTGTT GATTGACATG ACCACAGGAG AGGTGGTGCA CATTGACTAC 
AATGTATGCT TTGAGAAAGG GAAGAGTCTA CGAGTGCCAG AGAAAGTCCC ATTCAGGATG 
ACCCACAACA TTGAGACTGC TCTGGGTGTC ACCGGTGTGG AGGGCATCTT CAGGCTGTCC 
TGTGAACAGG TTCTTCAGAT CATGCGTCGT GGCAGAGAGA CCCTGTTGAC CCTGCTGGAG 
GCCTTTGTCT ACGATCCTCT TGTGGACTGG ACTGCTGGTG GGGAAGTGGG TTTTGCTGGC 
GCTGTTTATG GAGGAGGAGG CCAACAAGCT GAAAACAAGC AGAGCAAAAG GGAGATGGAA 
AGGGACATTA CACGCTCTCT TTTCTCATCC AGGGTAGCTG AGATTAAGGT GAATTGGTTT 
AAAAATCGTG ATGAAATGCT GGCAGTGTTG CCACAAGTGG AAGAGGCTGT GGAAGAGTAC 
CTGGTTCTGC AGGAGCTGCT CTGCCAGGGT GGGAAACTGC AGGACAAACT TCAAGAAGAG 
ATAACCTTCC TAGAAGGAGC AGAGAACCAT CCAGACCATC TTATTCTTAC CCTGGAGCAA 
AGGTACTCTG AACACACTCG GCTTCAGTCA CAGCAGCGAA CGATGCAGGA GGCCATTCAA 
AGCAAACTCA CTGACCTTGA CCAGTGGATT TCCCAGTACC AGGCAGCTTT CTCCAACCTG 
GAAGCTGCCC AGCTGGCCAC TTTGCTTCAA GACATCAGTT GCCCCATAGA TTTGGGTCCT 
CCCAGCTATG TACCTGCTAC AGCCTTCCTA CAGAATGCAG GCCAGGCCCA TTTGATCACC 
CAGTGTGAGA ACTTGGAAGC AGAATTGAGC ACTTTGCTGC AGCAGCGACG TGGGGCCCTT 
CGTGGTTGCC TAGAGCAGTT GCACAGCTAC GCAACAGTAG CATTACTGTA CCCAAGAGGT 
ACTTTGCGGC TCCATCGAGT CCAGCAGTGG AAGGCCTGGT TAGAGGAATT GTTGACAGAC 
ATGACGGTGG AGCAGTGCCA GCATATCTAC CGACAATATG AGATACTCTT TGCACCTCAG 
CCTTCTGCAG CTATCTGTCA GTTTTTGTCA AGTGTGGAGT TGACTTTGCA ACATCAGATG 
GCAGAAACCA ATGATCGTGT GATGCATCAG GCAGAGCGGC TCAAGGCCGA GGGAACGACT 
GTGGCAGTGT GCGAAGATCA AGTACAGGAG ATTGACCACT GTATCACAGG TTTTCTACAT 
GAGAACAGAG AGTATGGTTC TCTGGGATTG GCAGGAATCA TCACCTCTCT CCTGTGTACA 
CTGTGCAGAA GAAATCTGGT TATGGAAACT GCTGCAGCCA ATGCTGGAGA ACAGCTTATT 
GACTTAAAGT CTCGAGATGG GACATGGTTC CTGGAGGAGC TCTGCAGTAT GTTTGGAAAT 
GTCAGTGGCC TGACAACACT TCTACAACGC TGCCAACTTG TACAACATGA TCTGGAGCTG 
CCTGCCCTCT CAGACACCAT CCAAGCTGTT TATCTGGCCA ATGTTGTATA TACCTGCTTA 
CAAGAACTAA ACACCAATTT TCGACAGATC ATCTTTCCAG AAGCTTTGTG TTGTATGATA 
AAAGGAGAGC CCACTCTCGA GTCCATGTTG GTGGAGATAG AGCAGCTGGT GGAGCAGAGC 
TCTGATGGTC TTGGACTGCA GGGATTGGTT GACAGTCTGC AATCTACTAT GCGGTTCGAC 
CAAGATGGAC ACAACCATTT GCTTCACATT ACAAGTTTAC TGCGTGCTCA GTACTCTGGG 
CTGATCCAGC CTCGGAACAC AGAAGGACCA GGTCACGACA CACCAAAGAT GTCCCCAGGA 
CAAATGTTGT TGGTGGCGTT TGATGGAATG TTCACCCAGC TAGAGAATGC ATTTATGCAG 
CTCACAGAGA AGCTGAGTTG TCTCGAGGTG CCTTCAGCCT GGCAAAAAAT AGATTCTTTG 
AGGGAAGCTC GGACCGCACA GCTCTATTTC TTTGAGAATC GGAGTCAGAG ACAACTCATG 
GAAGAGGTAT TCTTCCTCAA AAGACTGCAG ACCATTAGGG ATTTCTTCAA GCTTTGTGCC 
ACATTTGCCC AAACTATCTC TGGTACCTGT CCTTCAACTG AGGACCTCTT AACAAATGGG 
CCAGTAGGAT CGGGGAAGTC ATTCTACCAG CTGCCCACTG CATCAGACTT GGGTGTGCCA 
GTGGTCAGTG AAGAAAAGAT GACCCATCCG ATTAAGGCCT TCACAGCTGA GTTTGTCCGA 
CAGATGCTCA TGGGTCTGCC CAGCCAAACA CTAAGCCTAG TTCTGTGCAG CGCCCTTTCT 
TCTCTTGGGC TGGATATTGT TTCCCTGGTG GAGGCCAAAG ACTTTGGTGT TGAGAGAAAG 
GTTTCTCTGG ATGAACTTTG TAAGACGGCA GTGGAGCAGA GCCTAGCTTC AGGCCGGGTT 
TCCCAGCTGT TGTTGAACAG AGCCACTGTA TTGGCCTCCT CCTATGATAC AGCTTGGAAG 
AAACTAGACC TTCTCAGAAG ACTGGATGCC CACCTGGAGG CTGCCAAGGC CAGTTTGCAG 
CGAAGCCAGC TGCATATCGC CATGTTTCAG TGGCATCATG AGGATATTCT GGGCCCCAGA 
AATCAGCCTC TAGGTGTAAG CATTCCACCT CGTACCATCA TTCTTAGCAA CATGAAGAAA 
AAACTTTACA AGCTGAGTCA GGATGAGGGA GCCATAGTTG CTGTTCAGGA GAAGCTGGCG 
TCACTGGAGG CTAGCATTGA GCAACGACTA AAGTGGGCAG GTGGAGCCAA TCCAGCACTG 
GCCCCAGTAC TGCAAGACTT TGAGATGACC ATCACTGAGA GACGTGCAAT TGTGGCCAGA 
GAAAACCAGC GCACCAGCCA GGTCACTTTT CTGTGCTCAA CTATCCTTAA TTTTGAAGGC 
CTTCGTACCC GAACACCTGA GGCCCTGAAT ATGGATGCAG CACTTTTTGA GTTGTTAAAA 
CGTTGCCAGC AAACCTGCTC CTTTGCTGCC CAGTTTAGCA GCAGTGTGTC TCCACTTGAG 
CTTCAGCTGT TGCAAAGGCT GAGTCCAGTG CTTGACTTGC CTATTGGCAG CCCCGACTGG 
CTGAGCTGTG CACAAAAGCA ACTGGAGGAG CAGCTTAATG TTGAGCAAAG CACTCGGGAG 
GAGCGGGAAC AGCAGCTGGA GGTCTGTGCA GAGACTCTGC AAGTTCTGGT CGATTCTGTA 
AAGACCATCC TGTCCCATCA CAACCGCCAG CTGGCAGATG TCAAGCACCT ATTGAGAGCC 
ATGGCAAAGG ACGAGGAGGC AGCTTTGGCA GATGGTGAAG AGGTGATATA TGAGGGTAGC 
GTGCGTCAGT TTCTGCTGGA GTACAAAGCA TGGCAAGACA ACATCCAGCT GGTTTTATTC 
ACTGTGGTCC AGGCTAGTGG ACAGCCTCGC AGTCAGGAGC AGCTTGAGCT GCTGGAAGAC 
ATTCCTGACA CGCTGAAGGA ACTGTACTCT CAAAGTGAGA GTGTGTATAA TGGCTTAGTT 
GGCTTCGCTT CCCCACTCGT GACTGACCGT GAAAGTGAAA GCACCAGTCC AGTGTCTGCA 
ATACAGACGA GCTTTGCAGC AGCTGTCAGG TGCACTGGGT TAAAGACCCA GCCAGACTCG 
GTCTCCCAGA ATGTTCGCAA GGCTCTGCCG CGGAATCTCG GAACCCCTAC TGATACCCCG 
CCCAGTGCTC CGTTGGTGAA CAACAAGTGT CTCAATGCCA GCCCAAAGAG AGCCATACGT 
GACCCCAAAA CTGGACGAGC TGTTCAGGAG AGAAACTCCT ATGCAGTCAG TGTGTGGAAG 
CGAGTGAAAG CCAAACTTGA GGGAAGAGAT ATTGATCCGA ACAGAAGGAT GAGTATCACA 
GAGCAGGTGG ACTTTGTCAT CAAAGAGGCC ACAAATCTGG ACAACCTTGC GCAGCTATAC 
GAAGGCTGGA CTGCCTGGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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smg1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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