ankle1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	ankle1
Entrez Gene ID	101065376
Full Name	uncharacterized LOC101065376
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011614982.1	XP_011613284.1	uncharacterized protein LOC101065376
XM_011614982.2	XP_011613284.2	ankyrin repeat and LEM domain-containing protein 1

Pathways from BioSystems
Gene Ontology

The following ankle1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankle1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo21800	XM_011614982.1 Latest version!	Takifugu rubripes uncharacterized LOC101065376 (LOC101065376), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo58873	XM_011614982.2 Latest version!	Takifugu rubripes ankyrin repeat and LEM domain containing 1 (ankle1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ankle1 ( XM_011614982.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011613284.1 Takifugu rubripes uncharacterized LOC101065376 (LOC101065376), mRNA.

Price & Availability↑

CloneID	OTo21800
Clone ID Related Accession (Same CDS sequence)	XM_011614982.1
Accession Version	XM_011614982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	uncharacterized protein LOC101065376
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071148.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGA AGACGTCGAG GCTGGAAAGT CAGCTTTGCC AAGCGGTGAA GAACGGAGAT 
CCCAGATCTG TGCAGCTGCT GCTTTCACAA GGTGCATGCC CGGACCTGGT GGGCATCAAA 
GGGGCGGCAG CAATACACCT GGCTGTGGGC AGAGAGACCG AGAGAAACAC ACGCTGCTTG 
AAGCTGCTGC TGCAGCATGG AGCCGACCCC AACGTCAAGT CATCAGAAGG ACTGACGCCA 
CTTCATATTG CTGCAGTTTG GGGATGTTAT CAAAACCTTA AGCTGCTCTT GACGAATGGC 
GGCAACCCAA AAATCAAAGA TAATGATGGG AATACACCAA GGCAGCTTGC AGAGCAACAG 
GAGAATAGGA AATGTGCCCT GCTCCTCCAG GAATATCAGG GCAGCTCAGC AGACAAAAAT 
GATGATGACT TTCCTCAGTT TCAGTACTCT TTGTATTCGG ACCAGACGGA CACATCCGGC 
TACCCTGAAT CGGATCACAG CTATATTTCC AACTCCTCTA TCATGAGTGA CTTTGGTGAG 
GCCGTGCTGA GCAGCACGAG GCGATCGTCA TTGCTCGCCA TATCTCACAT GAACGGAAGG 
CCAGGTTGCA GAGTAGTCTC TGATATTGAG ACAAAGGCAT GCCACCTGAA CTGTTCCAGC 
TCAGCCTGGA GACCCGATTG TCCCTCCATA CTGTCAAGCA CACGTGTGTC TGCAGCTGGA 
TCCGTAGCGC CGCTGCAGGT TATTAAAGAA CATCAGTTGG ATACGAATGT TGAGACAACC 
GCAGTAAAAG CAAAACAGTG TTCTGCTTCT TCAGATGGCA AAGACTCTCC CTCCCGAAGA 
GACACTCTTC CTGCATTTCA CAGGAGGCAA ATAGGTCACA AGAGCGTTAG CTTCAGAGAT 
GTAGATGAGT ATTTCCCTGT TTTTAGTCCC GAAGCTCCTG GAGTGCAGCC TGATGGCAGA 
TGTCCAGGCT TCGATCTGTC TGAGTATTCC GACTTCCTGG ATACAGAACG CATGGCCACT 
GTTCTCCCCC AGCAGGGCAT CGACGTCACG TCGCCGGATC ACGTGTACGT CTTTTTCAGG 
GAGAGCAGCG AGAGCACGGA AGAAGACATG GAGAAAACGG TCATGAGCCG CTTCCCTTTG 
GAGGAGAGTG ATAGAGAAAA GGACACCGAC CCTGCAAAGG ATGCGGCTTC AAAAAAAGTT 
GCTTCTCCAC CTGTTGGCAG CAGTAGCTCT GGTACTGGGA GTAGCCATTA CAGTAGCTGT 
GATAGTGACC ATTATACCAG TGCTCTGGAT GCTTCCGTGC ATCCCAGAGG TTGTTTAGTT 
TCTGACGGAG AGGAGAGCTC TGCTGGAGCT GAGCCTGACA GAAATACTAA GATTTCATCA 
CAGTCAGAGC AACAATGCAG TCGTTCAGAT GTCCAAACAG AAGCTCAGGA TGAAAAAAAC 
ACAGAACCTT TAACAGATGT TGGAGATGCA TTTGATTCAC CTGGAAAGAA GCCCCGTGAC 
AGCAATGAAG ATAAAACATG CACAACTTCC ATGGAGAGTG GGATCGATCA ACCAGGAAAT 
GGTGCAGCTG AGGACAACTT TGACCTCACA CTCACCCACA GCCCGTTTGT AACAGGCCGG 
ACCCGTTCCA GGATGACTCG CTACTCACTG AGAAAAAGCA AAACCCCCGA GAGCCTGCTC 
TTTACGTCCT CTCTCTTTGA TGACTCCCTC CCGACACCGG TCCGAACACG GCGCCAGACG 
CCCCGGGCGC AGAGCACAGA AGATTTGTAC AGCTCACCAC GTGCACCATG TTATATAGCC 
GGCCTTTCAG ACACTACAGA AGACTCTTTG AGTGCCGATT GGCAGGACAC GAAGCCCAGC 
ACCCTTGGAC CCGGCTCCAG TGTTGGCAAC AGCCAGGCTG ACACACTCAT TCTCTCTAAG 
AGCACGAGTG ATTCTGTTGA AACGCAGACT TTGTCCGACA CGGTCGTACT GGAGAAAAAC 
GAGGACTCTT CTGTGAGCAC TTACGAAAGA AACCTTGCGG ATGTTATACT GGCCATGCGA 
GGCCAGGCGC TTACCGAGGA CGAGGATCTA CTGACTGACG ATCTCACGAG CGCAGAGGAG 
GCAGCGCCAA ACCAGAAACT AAAGGACGGC GTCGAGAGCC TTTCAGAAGA CGCATGGATC 
TCCGAGGAGT CACAGTCTGA CTCTGCTTCA GCTTCATCCA GCTCCAGTTG TTTTTCTCCG 
AGGGGATCCA GTGAGAACTC CGACATTCCT TGTACTCCAG GCACCGGCTG CACCCCCAGA 
TACAGCATGA GCCGCCTGTC GAGCTGCAGT AGGCCTCAGC CCCTTGCGAA CCTGTCCTAC 
ACCCCCGGAG GCCGTCCGCA CATCCTGGAT CTGGAGGAGC CGGTGGAGTA CCTGTACACG 
GATACGGAAC AGGGCCACAA GCTGATCGAG ACCCACATCC CACCAACCTC AAACACCTCG 
CTCAGTTCCA GCATGAGTAC CTCCAGCGGT GATGAGACCG TCCTCTACGA CTGGCGGTCC 
ATGCAGGCGG ACATTAAAAA GAGCGGAGGA AAGGAGAACC AGAAACCTCC CACAGAGCCG 
AAGAAAGAAG AGAAGTGTGA GGTTGTGCTT CCCGAGACGG CAGGGATGAC TGACAGAGAG 
CTCAGACTGA GGCTGGTGGA GCTGGGCGAG AGCCCAGGCC CCATCAGCAG CCGCACCAGA 
CCCACCTACA TGCGAAGGCT GTGTCGTCTG ATGAAGGAGT CAAACTCTCA GTCACCACGG 
CAACAAAGGC AGACCGACCA ACCCCAAACT GATTTAGGTT ATACCCCAGA GTTGCGTCTG 
GCCCTGCGGA CCTTTGAGCT GCCCCACTGC CATGCTGACG AGCAGGCTCT CTGCCAGCAG 
TTCGACCAGG CGGATCAAAA CAGGAAGTGG AGGGAAGGCG TCATTAAATC CTGCTTCAGC 
TACTTGCTAC TTGACCCAAG GGTGACCAAA AACCTCCCGT TCCGCAGCCA CACCATGAGC 
CCACAGGAGT GTTTCCAGAC ATTTGTTAAC GCCATATTTT ACGTGGGCAA AGGAAAACGC 
TCCCGTCCCT ACAGCCACCT GTACGAGGCT TTAGAGTACC ACCGAGGGGA CAAAATGTCC 
AAGAAATTGT GCCCCAAAGT GCAGCACATC CTTCAGGTGT GGAACTCCCA GCAGGGCATC 
ATCTCTCTGC ACTGCTTCCA GAACGTGATT CCAGTGGAGG CGTACACCAG AGAGGCGTGC 
ATGGTGGAGG CTATCGGGTT AAAGATGCTC ACCAATCAGA AGAGAGGCGA TTTTTACGGC 
GTGGTGTCCA ACTGGCCGCT GAACAGGAAA CGAGAGCTCG GCATCCATCT GCTCTACCGG 
GCCATGCACA TCTTTTTGGC CGAGGGTGAG AGGCAGCTCA GGCCGGTAGA CATCAGACAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011613284.1
CDS	309..3731
Translation	MDQKTSRLESQLCQAVKNGDPRSVQLLLSQGACPDLVGIKGAAAIHLAVGRETERNTRCLKLLLQHGADPNVKSSEGLTPLHIAAVWGCYQNLKLLLTNGGNPKIKDNDGNTPRQLAEQQENRKCALLLQEYQGSSADKNDDDFPQFQYSLYSDQTDTSGYPESDHSYISNSSIMSDFGEAVLSSTRRSSLLAISHMNGRPGCRVVSDIETKACHLNCSSSAWRPDCPSILSSTRVSAAGSVAPLQVIKEHQLDTNVETTAVKAKQCSASSDGKDSPSRRDTLPAFHRRQIGHKSVSFRDVDEYFPVFSPEAPGVQPDGRCPGFDLSEYSDFLDTERMATVLPQQGIDVTSPDHVYVFFRESSESTEEDMEKTVMSRFPLEESDREKDTDPAKDAASKKVASPPVGSSSSGTGSSHYSSCDSDHYTSALDASVHPRGCLVSDGEESSAGAEPDRNTKISSQSEQQCSRSDVQTEAQDEKNTEPLTDVGDAFDSPGKKPRDSNEDKTCTTSMESGIDQPGNGAAEDNFDLTLTHSPFVTGRTRSRMTRYSLRKSKTPESLLFTSSLFDDSLPTPVRTRRQTPRAQSTEDLYSSPRAPCYIAGLSDTTEDSLSADWQDTKPSTLGPGSSVGNSQADTLILSKSTSDSVETQTLSDTVVLEKNEDSSVSTYERNLADVILAMRGQALTEDEDLLTDDLTSAEEAAPNQKLKDGVESLSEDAWISEESQSDSASASSSSSCFSPRGSSENSDIPCTPGTGCTPRYSMSRLSSCSRPQPLANLSYTPGGRPHILDLEEPVEYLYTDTEQGHKLIETHIPPTSNTSLSSSMSTSSGDETVLYDWRSMQADIKKSGGKENQKPPTEPKKEEKCEVVLPETAGMTDRELRLRLVELGESPGPISSRTRPTYMRRLCRLMKESNSQSPRQQRQTDQPQTDLGYTPELRLALRTFELPHCHADEQALCQQFDQADQNRKWREGVIKSCFSYLLLDPRVTKNLPFRSHTMSPQECFQTFVNAIFYVGKGKRSRPYSHLYEALEYHRGDKMSKKLCPKVQHILQVWNSQQGIISLHCFQNVIPVEAYTREACMVEAIGLKMLTNQKRGDFYGVVSNWPLNRKRELGIHLLYRAMHIFLAEGERQLRPVDIRQ

Target ORF information:

RefSeq Version	XM_011614982.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes uncharacterized LOC101065376 (LOC101065376), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011614982.1

ORF Insert Sequence:

ATGGATCAGA AGACGTCGAG GCTGGAAAGT CAGCTTTGCC AAGCGGTGAA GAACGGAGAT 
CCCAGATCTG TGCAGCTGCT GCTTTCACAA GGTGCATGCC CGGACCTGGT GGGCATCAAA 
GGGGCGGCAG CAATACACCT GGCTGTGGGC AGAGAGACCG AGAGAAACAC ACGCTGCTTG 
AAGCTGCTGC TGCAGCATGG AGCCGACCCC AACGTCAAGT CATCAGAAGG ACTGACGCCA 
CTTCATATTG CTGCAGTTTG GGGATGTTAT CAAAACCTTA AGCTGCTCTT GACGAATGGC 
GGCAACCCAA AAATCAAAGA TAATGATGGG AATACACCAA GGCAGCTTGC AGAGCAACAG 
GAGAATAGGA AATGTGCCCT GCTCCTCCAG GAATATCAGG GCAGCTCAGC AGACAAAAAT 
GATGATGACT TTCCTCAGTT TCAGTACTCT TTGTATTCGG ACCAGACGGA CACATCCGGC 
TACCCTGAAT CGGATCACAG CTATATTTCC AACTCCTCTA TCATGAGTGA CTTTGGTGAG 
GCCGTGCTGA GCAGCACGAG GCGATCGTCA TTGCTCGCCA TATCTCACAT GAACGGAAGG 
CCAGGTTGCA GAGTAGTCTC TGATATTGAG ACAAAGGCAT GCCACCTGAA CTGTTCCAGC 
TCAGCCTGGA GACCCGATTG TCCCTCCATA CTGTCAAGCA CACGTGTGTC TGCAGCTGGA 
TCCGTAGCGC CGCTGCAGGT TATTAAAGAA CATCAGTTGG ATACGAATGT TGAGACAACC 
GCAGTAAAAG CAAAACAGTG TTCTGCTTCT TCAGATGGCA AAGACTCTCC CTCCCGAAGA 
GACACTCTTC CTGCATTTCA CAGGAGGCAA ATAGGTCACA AGAGCGTTAG CTTCAGAGAT 
GTAGATGAGT ATTTCCCTGT TTTTAGTCCC GAAGCTCCTG GAGTGCAGCC TGATGGCAGA 
TGTCCAGGCT TCGATCTGTC TGAGTATTCC GACTTCCTGG ATACAGAACG CATGGCCACT 
GTTCTCCCCC AGCAGGGCAT CGACGTCACG TCGCCGGATC ACGTGTACGT CTTTTTCAGG 
GAGAGCAGCG AGAGCACGGA AGAAGACATG GAGAAAACGG TCATGAGCCG CTTCCCTTTG 
GAGGAGAGTG ATAGAGAAAA GGACACCGAC CCTGCAAAGG ATGCGGCTTC AAAAAAAGTT 
GCTTCTCCAC CTGTTGGCAG CAGTAGCTCT GGTACTGGGA GTAGCCATTA CAGTAGCTGT 
GATAGTGACC ATTATACCAG TGCTCTGGAT GCTTCCGTGC ATCCCAGAGG TTGTTTAGTT 
TCTGACGGAG AGGAGAGCTC TGCTGGAGCT GAGCCTGACA GAAATACTAA GATTTCATCA 
CAGTCAGAGC AACAATGCAG TCGTTCAGAT GTCCAAACAG AAGCTCAGGA TGAAAAAAAC 
ACAGAACCTT TAACAGATGT TGGAGATGCA TTTGATTCAC CTGGAAAGAA GCCCCGTGAC 
AGCAATGAAG ATAAAACATG CACAACTTCC ATGGAGAGTG GGATCGATCA ACCAGGAAAT 
GGTGCAGCTG AGGACAACTT TGACCTCACA CTCACCCACA GCCCGTTTGT AACAGGCCGG 
ACCCGTTCCA GGATGACTCG CTACTCACTG AGAAAAAGCA AAACCCCCGA GAGCCTGCTC 
TTTACGTCCT CTCTCTTTGA TGACTCCCTC CCGACACCGG TCCGAACACG GCGCCAGACG 
CCCCGGGCGC AGAGCACAGA AGATTTGTAC AGCTCACCAC GTGCACCATG TTATATAGCC 
GGCCTTTCAG ACACTACAGA AGACTCTTTG AGTGCCGATT GGCAGGACAC GAAGCCCAGC 
ACCCTTGGAC CCGGCTCCAG TGTTGGCAAC AGCCAGGCTG ACACACTCAT TCTCTCTAAG 
AGCACGAGTG ATTCTGTTGA AACGCAGACT TTGTCCGACA CGGTCGTACT GGAGAAAAAC 
GAGGACTCTT CTGTGAGCAC TTACGAAAGA AACCTTGCGG ATGTTATACT GGCCATGCGA 
GGCCAGGCGC TTACCGAGGA CGAGGATCTA CTGACTGACG ATCTCACGAG CGCAGAGGAG 
GCAGCGCCAA ACCAGAAACT AAAGGACGGC GTCGAGAGCC TTTCAGAAGA CGCATGGATC 
TCCGAGGAGT CACAGTCTGA CTCTGCTTCA GCTTCATCCA GCTCCAGTTG TTTTTCTCCG 
AGGGGATCCA GTGAGAACTC CGACATTCCT TGTACTCCAG GCACCGGCTG CACCCCCAGA 
TACAGCATGA GCCGCCTGTC GAGCTGCAGT AGGCCTCAGC CCCTTGCGAA CCTGTCCTAC 
ACCCCCGGAG GCCGTCCGCA CATCCTGGAT CTGGAGGAGC CGGTGGAGTA CCTGTACACG 
GATACGGAAC AGGGCCACAA GCTGATCGAG ACCCACATCC CACCAACCTC AAACACCTCG 
CTCAGTTCCA GCATGAGTAC CTCCAGCGGT GATGAGACCG TCCTCTACGA CTGGCGGTCC 
ATGCAGGCGG ACATTAAAAA GAGCGGAGGA AAGGAGAACC AGAAACCTCC CACAGAGCCG 
AAGAAAGAAG AGAAGTGTGA GGTTGTGCTT CCCGAGACGG CAGGGATGAC TGACAGAGAG 
CTCAGACTGA GGCTGGTGGA GCTGGGCGAG AGCCCAGGCC CCATCAGCAG CCGCACCAGA 
CCCACCTACA TGCGAAGGCT GTGTCGTCTG ATGAAGGAGT CAAACTCTCA GTCACCACGG 
CAACAAAGGC AGACCGACCA ACCCCAAACT GATTTAGGTT ATACCCCAGA GTTGCGTCTG 
GCCCTGCGGA CCTTTGAGCT GCCCCACTGC CATGCTGACG AGCAGGCTCT CTGCCAGCAG 
TTCGACCAGG CGGATCAAAA CAGGAAGTGG AGGGAAGGCG TCATTAAATC CTGCTTCAGC 
TACTTGCTAC TTGACCCAAG GGTGACCAAA AACCTCCCGT TCCGCAGCCA CACCATGAGC 
CCACAGGAGT GTTTCCAGAC ATTTGTTAAC GCCATATTTT ACGTGGGCAA AGGAAAACGC 
TCCCGTCCCT ACAGCCACCT GTACGAGGCT TTAGAGTACC ACCGAGGGGA CAAAATGTCC 
AAGAAATTGT GCCCCAAAGT GCAGCACATC CTTCAGGTGT GGAACTCCCA GCAGGGCATC 
ATCTCTCTGC ACTGCTTCCA GAACGTGATT CCAGTGGAGG CGTACACCAG AGAGGCGTGC 
ATGGTGGAGG CTATCGGGTT AAAGATGCTC ACCAATCAGA AGAGAGGCGA TTTTTACGGC 
GTGGTGTCCA ACTGGCCGCT GAACAGGAAA CGAGAGCTCG GCATCCATCT GCTCTACCGG 
GCCATGCACA TCTTTTTGGC CGAGGGTGAG AGGCAGCTCA GGCCGGTAGA CATCAGACAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle1 ( XM_011614982.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011613284.2 Takifugu rubripes ankyrin repeat and LEM domain containing 1 (ankle1), mRNA.

Price & Availability↑

CloneID	OTo58873
Clone ID Related Accession (Same CDS sequence)	XM_011614982.2
Accession Version	XM_011614982.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	ankyrin repeat and LEM domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042304.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011614982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAGA AGACGTCGAG GCTGGAAAGT CAGCTTTGCC AAGCGGTGAA GAACGGAGAT 
CCCAGATCTG TGCAGCTGCT GCTTTCACAA GGTGCATGCC CGGACCTGGT GGGCATCAAA 
GGGGCGGCAG CAATACACCT GGCTGTGGGC AGAGAGACCG AGAGAAACAC ACGCTGCTTG 
AAGCTGCTGC TGCAGCATGG AGCCGACCCC AACGTCAAGT CATCAGAAGG ACTGACGCCA 
CTTCATATTG CTGCAGTTTG GGGATGTTAT CAAAACCTTA AGCTGCTCTT GACGAATGGC 
GGCAACCCAA AAATCAAAGA TAATGATGGG AATACACCAA GGCAGCTTGC AGAGCAACAG 
GAGAATAGGA AATGTGCCCT GCTCCTCCAG GAATATCAGG GCAGCTCAGC AGACAAAAAT 
GATGATGACT TTCCTCAGTT TCAGTACTCT TTGTATTCGG ACCAGACGGA CACATCCGGC 
TACCCTGAAT CGGATCACAG CTATATTTCC AACTCCTCTA TCATGAGTGA CTTTGGTGAG 
GCCGTGCTGA GCAGCACGAG GCGATCGTCA TTGCTCGCCA TATCTCACAT GAACGGAAGG 
CCAGGTTGCA GAGTAGTCTC TGATATTGAG ACAAAGGCAT GCCACCTGAA CTGTTCCAGC 
TCAGCCTGGA GACCCGATTG TCCCTCCATA CTGTCAAGCA CACGTGTGTC TGCAGCTGGA 
TCCGTAGCGC CGCTGCAGGT TATTAAAGAA CATCAGTTGG ATACGAATGT TGAGACAACC 
GCAGTAAAAG CAAAACAGTG TTCTGCTTCT TCAGATGGCA AAGACTCTCC CTCCCGAAGA 
GACACTCTTC CTGCATTTCA CAGGAGGCAA ATAGGTCACA AGAGCGTTAG CTTCAGAGAT 
GTAGATGAGT ATTTCCCTGT TTTTAGTCCC GAAGCTCCTG GAGTGCAGCC TGATGGCAGA 
TGTCCAGGCT TCGATCTGTC TGAGTATTCC GACTTCCTGG ATACAGAACG CATGGCCACT 
GTTCTCCCCC AGCAGGGCAT CGACGTCACG TCGCCGGATC ACGTGTACGT CTTTTTCAGG 
GAGAGCAGCG AGAGCACGGA AGAAGACATG GAGAAAACGG TCATGAGCCG CTTCCCTTTG 
GAGGAGAGTG ATAGAGAAAA GGACACCGAC CCTGCAAAGG ATGCGGCTTC AAAAAAAGTT 
GCTTCTCCAC CTGTTGGCAG CAGTAGCTCT GGTACTGGGA GTAGCCATTA CAGTAGCTGT 
GATAGTGACC ATTATACCAG TGCTCTGGAT GCTTCCGTGC ATCCCAGAGG TTGTTTAGTT 
TCTGACGGAG AGGAGAGCTC TGCTGGAGCT GAGCCTGACA GAAATACTAA GATTTCATCA 
CAGTCAGAGC AACAATGCAG TCGTTCAGAT GTCCAAACAG AAGCTCAGGA TGAAAAAAAC 
ACAGAACCTT TAACAGATGT TGGAGATGCG TTTGATTCAC CTGGAAAGAA GCCCCGTGAC 
AGCAATGAAG ATAAAACATG CACAACTTCC ATGGAGAGTG GGATCGATCA ACCAGGAAAT 
GGTGCAGCTG AGGACAACTT TGACCTCACA CTCACCCACA GCCCGTTTGT AACAGGCCGG 
ACCCGTTCCA GGATGACTCG CTACTCACTG AGAAAAAGCA AAACCCCCGA GAGCCTGCTC 
TTTACGTCCT CTCTCTTTGA TGACTCCCTC CCGACACCGG TCCGAACACG GCGCCAGACG 
CCCCGGGCGC AGAGCACAGA AGATTTGTAC AGCTCACCAC GTGCACCATG TTATATAGCC 
GGCCTTTCAG ACACTACAGA AGACTCTTTG AGTGCCGATT GGCAGGACAC GAAGCCCAGC 
ACCCTTGGAC CCGGCTCCAG TGTTGGCAAC AGCCAGGCTG ACACACTCAT TCTCTCTAAG 
AGCACGAGTG ATTCTGTTGA AACGCAGACT TTGTCCGACA CGGTCGTACT GGAGAAAAAC 
GAGGACTCTT CTGTGAGCAC TTACGAAAGA AACCTTGCGG ATGTTATACT GGCCATGCGA 
GGCCAGGCGC TTACCGAGGA CGAGGATCTA CTGACTGACG ATCTCACGAG CGCAGAGGAG 
GCAGCACCAA ACCAGAAACT AAAGGACGGC GTCGAGAGCC TTTCAGAAGA CGCATGGATC 
TCCGAGGAGT CACAGTCTGA CTCTGCTTCA GCTTCATCCA GCTCCAGTTG TTTTTCTCCG 
AGGGGATCCA GTGAGAACTC CGACATTCCT TGTACTCCAG GCACCGGCTG CACCCCCAGA 
TACAGCATGA GCCGCCTGTC GAGCTGCAGT AGGCCTCAGC CCCTTGCGAA CCTGTCCTAC 
ACCCCCGGAG GCCGTCCGCA CATCCTGGAT CTGGAGGAGC CGGTGGAGTA CCTGTACACG 
GATACGGAAC AGGGCCACAA GCTGATCGAG ACCCACATCC CACCAACCTC AAACACCTCG 
CTCAGTTCCA GCATGAGTAC CTCCAGCGGT GATGAGACCG TCCTCTACGA CTGGCGGTCC 
ATGCAGGCGG ACATTAAAAA GAGCGGAGGA AAGGAGAACC AGAAACCTCC CACAGAGCCG 
AAGAAAGAAG AGAAGTGTGA GGTTGTGCTT CCCGAGACGG CAGGGATGAC TGACAGAGAG 
CTCAGACTGA GGCTGGTGGA GCTGGGCGAG AGCCCAGGCC CCATCAGCAG CCGCACCAGA 
CCCACCTACA TGCGAAGGCT GTGTCGTCTG ATGAAGGAGT CAAACTCTCA GTCACCACGG 
CAACAAAGGC AGACCGACCA ACCCCAAACT GATTTAGGTT ATACCCCAGA GTTGCGTCTG 
GCCCTGCGGA CCTTTGAGCT GCCCCACTGC CATGCTGACG AGCAGGCTCT CTGCCAGCAG 
TTCGACCAGG CGGATCAAAA CAGGAAGTGG AGGGAAGGCG TCATTAAATC CTGCTTCAAC 
TACTTGCTAC TTGACCCAAG GGTGACCAAA AACCTCCCGT TCCGCAGCCA CACCATGAGC 
CCACAGGAGT GTTTCCAGAC ATTTGTTAAC GCCATATTTT ACGTGGGCAA AGGAAAACGC 
TCCCGTCCCT ACAGCCACCT GTACGAGGCT TTAGAGTACC ACCGAGGGGA CAAAATGTCC 
AAGAAATTGT GCCCCAAAGT GCAGCACATC CTTCAGGTGT GGAACTCCCA GCAGGGCATC 
ATCTCTCTGC ACTGCTTCCA GAACGTGATT CCAGTGGAGG CGTACACCAG AGAGGCGTGC 
ATGGTGGAGG CTATCGGGTT AAAGATGCTC ACCAATCAGA AGAGAGGCGA TTTTTACGGC 
GTGGTGTCCA ACTGGCCGCT GAACAGGAAA CGAGAGCTCG GCATCCATCT GCTCTACCGG 
GCCATGCACA TCTTTTTGGC CGAGGGTGAG AGGCAGCTCA GGCCGGTAGA CATCAGACAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011613284.2
CDS	329..3751
Translation	MDQKTSRLESQLCQAVKNGDPRSVQLLLSQGACPDLVGIKGAAAIHLAVGRETERNTRCLKLLLQHGADPNVKSSEGLTPLHIAAVWGCYQNLKLLLTNGGNPKIKDNDGNTPRQLAEQQENRKCALLLQEYQGSSADKNDDDFPQFQYSLYSDQTDTSGYPESDHSYISNSSIMSDFGEAVLSSTRRSSLLAISHMNGRPGCRVVSDIETKACHLNCSSSAWRPDCPSILSSTRVSAAGSVAPLQVIKEHQLDTNVETTAVKAKQCSASSDGKDSPSRRDTLPAFHRRQIGHKSVSFRDVDEYFPVFSPEAPGVQPDGRCPGFDLSEYSDFLDTERMATVLPQQGIDVTSPDHVYVFFRESSESTEEDMEKTVMSRFPLEESDREKDTDPAKDAASKKVASPPVGSSSSGTGSSHYSSCDSDHYTSALDASVHPRGCLVSDGEESSAGAEPDRNTKISSQSEQQCSRSDVQTEAQDEKNTEPLTDVGDAFDSPGKKPRDSNEDKTCTTSMESGIDQPGNGAAEDNFDLTLTHSPFVTGRTRSRMTRYSLRKSKTPESLLFTSSLFDDSLPTPVRTRRQTPRAQSTEDLYSSPRAPCYIAGLSDTTEDSLSADWQDTKPSTLGPGSSVGNSQADTLILSKSTSDSVETQTLSDTVVLEKNEDSSVSTYERNLADVILAMRGQALTEDEDLLTDDLTSAEEAAPNQKLKDGVESLSEDAWISEESQSDSASASSSSSCFSPRGSSENSDIPCTPGTGCTPRYSMSRLSSCSRPQPLANLSYTPGGRPHILDLEEPVEYLYTDTEQGHKLIETHIPPTSNTSLSSSMSTSSGDETVLYDWRSMQADIKKSGGKENQKPPTEPKKEEKCEVVLPETAGMTDRELRLRLVELGESPGPISSRTRPTYMRRLCRLMKESNSQSPRQQRQTDQPQTDLGYTPELRLALRTFELPHCHADEQALCQQFDQADQNRKWREGVIKSCFNYLLLDPRVTKNLPFRSHTMSPQECFQTFVNAIFYVGKGKRSRPYSHLYEALEYHRGDKMSKKLCPKVQHILQVWNSQQGIISLHCFQNVIPVEAYTREACMVEAIGLKMLTNQKRGDFYGVVSNWPLNRKRELGIHLLYRAMHIFLAEGERQLRPVDIRQ

Target ORF information:

RefSeq Version	XM_011614982.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes ankyrin repeat and LEM domain containing 1 (ankle1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011614982.2

ORF Insert Sequence:

ATGGATCAGA AGACGTCGAG GCTGGAAAGT CAGCTTTGCC AAGCGGTGAA GAACGGAGAT 
CCCAGATCTG TGCAGCTGCT GCTTTCACAA GGTGCATGCC CGGACCTGGT GGGCATCAAA 
GGGGCGGCAG CAATACACCT GGCTGTGGGC AGAGAGACCG AGAGAAACAC ACGCTGCTTG 
AAGCTGCTGC TGCAGCATGG AGCCGACCCC AACGTCAAGT CATCAGAAGG ACTGACGCCA 
CTTCATATTG CTGCAGTTTG GGGATGTTAT CAAAACCTTA AGCTGCTCTT GACGAATGGC 
GGCAACCCAA AAATCAAAGA TAATGATGGG AATACACCAA GGCAGCTTGC AGAGCAACAG 
GAGAATAGGA AATGTGCCCT GCTCCTCCAG GAATATCAGG GCAGCTCAGC AGACAAAAAT 
GATGATGACT TTCCTCAGTT TCAGTACTCT TTGTATTCGG ACCAGACGGA CACATCCGGC 
TACCCTGAAT CGGATCACAG CTATATTTCC AACTCCTCTA TCATGAGTGA CTTTGGTGAG 
GCCGTGCTGA GCAGCACGAG GCGATCGTCA TTGCTCGCCA TATCTCACAT GAACGGAAGG 
CCAGGTTGCA GAGTAGTCTC TGATATTGAG ACAAAGGCAT GCCACCTGAA CTGTTCCAGC 
TCAGCCTGGA GACCCGATTG TCCCTCCATA CTGTCAAGCA CACGTGTGTC TGCAGCTGGA 
TCCGTAGCGC CGCTGCAGGT TATTAAAGAA CATCAGTTGG ATACGAATGT TGAGACAACC 
GCAGTAAAAG CAAAACAGTG TTCTGCTTCT TCAGATGGCA AAGACTCTCC CTCCCGAAGA 
GACACTCTTC CTGCATTTCA CAGGAGGCAA ATAGGTCACA AGAGCGTTAG CTTCAGAGAT 
GTAGATGAGT ATTTCCCTGT TTTTAGTCCC GAAGCTCCTG GAGTGCAGCC TGATGGCAGA 
TGTCCAGGCT TCGATCTGTC TGAGTATTCC GACTTCCTGG ATACAGAACG CATGGCCACT 
GTTCTCCCCC AGCAGGGCAT CGACGTCACG TCGCCGGATC ACGTGTACGT CTTTTTCAGG 
GAGAGCAGCG AGAGCACGGA AGAAGACATG GAGAAAACGG TCATGAGCCG CTTCCCTTTG 
GAGGAGAGTG ATAGAGAAAA GGACACCGAC CCTGCAAAGG ATGCGGCTTC AAAAAAAGTT 
GCTTCTCCAC CTGTTGGCAG CAGTAGCTCT GGTACTGGGA GTAGCCATTA CAGTAGCTGT 
GATAGTGACC ATTATACCAG TGCTCTGGAT GCTTCCGTGC ATCCCAGAGG TTGTTTAGTT 
TCTGACGGAG AGGAGAGCTC TGCTGGAGCT GAGCCTGACA GAAATACTAA GATTTCATCA 
CAGTCAGAGC AACAATGCAG TCGTTCAGAT GTCCAAACAG AAGCTCAGGA TGAAAAAAAC 
ACAGAACCTT TAACAGATGT TGGAGATGCG TTTGATTCAC CTGGAAAGAA GCCCCGTGAC 
AGCAATGAAG ATAAAACATG CACAACTTCC ATGGAGAGTG GGATCGATCA ACCAGGAAAT 
GGTGCAGCTG AGGACAACTT TGACCTCACA CTCACCCACA GCCCGTTTGT AACAGGCCGG 
ACCCGTTCCA GGATGACTCG CTACTCACTG AGAAAAAGCA AAACCCCCGA GAGCCTGCTC 
TTTACGTCCT CTCTCTTTGA TGACTCCCTC CCGACACCGG TCCGAACACG GCGCCAGACG 
CCCCGGGCGC AGAGCACAGA AGATTTGTAC AGCTCACCAC GTGCACCATG TTATATAGCC 
GGCCTTTCAG ACACTACAGA AGACTCTTTG AGTGCCGATT GGCAGGACAC GAAGCCCAGC 
ACCCTTGGAC CCGGCTCCAG TGTTGGCAAC AGCCAGGCTG ACACACTCAT TCTCTCTAAG 
AGCACGAGTG ATTCTGTTGA AACGCAGACT TTGTCCGACA CGGTCGTACT GGAGAAAAAC 
GAGGACTCTT CTGTGAGCAC TTACGAAAGA AACCTTGCGG ATGTTATACT GGCCATGCGA 
GGCCAGGCGC TTACCGAGGA CGAGGATCTA CTGACTGACG ATCTCACGAG CGCAGAGGAG 
GCAGCACCAA ACCAGAAACT AAAGGACGGC GTCGAGAGCC TTTCAGAAGA CGCATGGATC 
TCCGAGGAGT CACAGTCTGA CTCTGCTTCA GCTTCATCCA GCTCCAGTTG TTTTTCTCCG 
AGGGGATCCA GTGAGAACTC CGACATTCCT TGTACTCCAG GCACCGGCTG CACCCCCAGA 
TACAGCATGA GCCGCCTGTC GAGCTGCAGT AGGCCTCAGC CCCTTGCGAA CCTGTCCTAC 
ACCCCCGGAG GCCGTCCGCA CATCCTGGAT CTGGAGGAGC CGGTGGAGTA CCTGTACACG 
GATACGGAAC AGGGCCACAA GCTGATCGAG ACCCACATCC CACCAACCTC AAACACCTCG 
CTCAGTTCCA GCATGAGTAC CTCCAGCGGT GATGAGACCG TCCTCTACGA CTGGCGGTCC 
ATGCAGGCGG ACATTAAAAA GAGCGGAGGA AAGGAGAACC AGAAACCTCC CACAGAGCCG 
AAGAAAGAAG AGAAGTGTGA GGTTGTGCTT CCCGAGACGG CAGGGATGAC TGACAGAGAG 
CTCAGACTGA GGCTGGTGGA GCTGGGCGAG AGCCCAGGCC CCATCAGCAG CCGCACCAGA 
CCCACCTACA TGCGAAGGCT GTGTCGTCTG ATGAAGGAGT CAAACTCTCA GTCACCACGG 
CAACAAAGGC AGACCGACCA ACCCCAAACT GATTTAGGTT ATACCCCAGA GTTGCGTCTG 
GCCCTGCGGA CCTTTGAGCT GCCCCACTGC CATGCTGACG AGCAGGCTCT CTGCCAGCAG 
TTCGACCAGG CGGATCAAAA CAGGAAGTGG AGGGAAGGCG TCATTAAATC CTGCTTCAAC 
TACTTGCTAC TTGACCCAAG GGTGACCAAA AACCTCCCGT TCCGCAGCCA CACCATGAGC 
CCACAGGAGT GTTTCCAGAC ATTTGTTAAC GCCATATTTT ACGTGGGCAA AGGAAAACGC 
TCCCGTCCCT ACAGCCACCT GTACGAGGCT TTAGAGTACC ACCGAGGGGA CAAAATGTCC 
AAGAAATTGT GCCCCAAAGT GCAGCACATC CTTCAGGTGT GGAACTCCCA GCAGGGCATC 
ATCTCTCTGC ACTGCTTCCA GAACGTGATT CCAGTGGAGG CGTACACCAG AGAGGCGTGC 
ATGGTGGAGG CTATCGGGTT AAAGATGCTC ACCAATCAGA AGAGAGGCGA TTTTTACGGC 
GTGGTGTCCA ACTGGCCGCT GAACAGGAAA CGAGAGCTCG GCATCCATCT GCTCTACCGG 
GCCATGCACA TCTTTTTGGC CGAGGGTGAG AGGCAGCTCA GGCCGGTAGA CATCAGACAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ankle1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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