LOC101064247 gene cDNA ORF clone, Takifugu rubripes(torafugu)

mRNA	Protein	Name
XM_011606377.1	XP_011604679.1	tensin-1 isoform X1
XM_011606378.1	XP_011604680.1	tensin-1 isoform X2
XM_029840193.1	XP_029696053.1	tensin isoform X5
XM_029840196.1	XP_029696056.1	tensin isoform X8
XM_029840200.1	XP_029696060.1	tensin isoform X10
XM_011606379.1	XP_011604681.1	tensin-1 isoform X3
XM_011606380.1	XP_011604682.1	tensin-1 isoform X4
XM_011606383.1	XP_011604685.1	tensin-1 isoform X6
XM_029840199.1	XP_029696059.1	tensin isoform X10
XM_011606382.1	XP_011604684.1	tensin-1 isoform X5
XM_029840201.1	XP_029696061.1	tensin isoform X10
XM_029840194.1	XP_029696054.1	tensin isoform X6
XM_029840189.1	XP_029696049.1	tensin isoform X1
XM_029840191.1	XP_029696051.1	tensin isoform X3
XM_029840192.1	XP_029696052.1	tensin isoform X4
XM_029840190.1	XP_029696050.1	tensin isoform X2
XM_029840197.1	XP_029696057.1	tensin isoform X9
XM_029840195.1	XP_029696055.1	tensin isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo20981	XM_011606377.1 Latest version!	Takifugu rubripes tensin 1 (tns1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo20982	XM_011606378.1 Latest version!	Takifugu rubripes tensin 1 (tns1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo20983	XM_011606379.1 Latest version!	Takifugu rubripes tensin 1 (tns1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo20984	XM_011606380.1 Latest version!	Takifugu rubripes tensin 1 (tns1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo20985	XM_011606382.1 Latest version!	Takifugu rubripes tensin 1 (tns1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo20986	XM_011606383.1 Latest version!	Takifugu rubripes tensin 1 (tns1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo71053	XM_029840189.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo71054	XM_029840190.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo71055	XM_029840191.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo71056	XM_029840192.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo71057	XM_029840193.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo71058	XM_029840194.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo71059	XM_029840195.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo71060	XM_029840196.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo71061	XM_029840197.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo71062	XM_029840199.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo71062	XM_029840200.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo71062	XM_029840201.1 Latest version!	Takifugu rubripes tensin (LOC101064247), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

LOC101064247 ( XM_011606377.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604679.1 Takifugu rubripes tensin 1 (tns1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo20981
Clone ID Related Accession (Same CDS sequence)	XM_011606377.1
Accession Version	XM_011606377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	tensin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070998.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG TCTCTCTGTC GTTGCCCTCC ACCCTGGCTG GGAGGACCAG GACCTGGGTT 
TGCCTCTCCT GCATGTTCTG GCCGGATGAG CTGGAGGGCG TCCACACTCA CACCTTCAAG 
CTGAAGCCAT TCAAGAAGGC CAAAAGCTGT GACATCTGTA AGCAGGCCAT CACCAAGGAG 
GGCCTCGTCT GCAAAGCGTG TCGGCTCTCC TGCCACAAAA AATGTGAAGT GAAGGTCACC 
ACATCATGTC AGACGTCCAC CAACCATGAG CTGCCCCCCA CTCCGCAGCT GCCATTAAAG 
CATGTAGACA CGCCGGGATC TGCGAGGTCC TGCAAGAGTG TGGAGATAAG GCGGAAGCAG 
TCTCGAAGCC AGAGTGTGGT TCTGACCATG GAGGAGAACT ATGAGGTAGA CCTGGTCTAC 
ATCACGGAGA GGATCATCTC CCTCTGCTTC CCCATTGGCA TGGAGGACCA GGGCTACATC 
GCCAACCTCA AGGAGGTGGC CACCATGCTG CGATCCAAAC ATGGAGATCA TTATTTGGTG 
CTGAACCTGA GTGAATGGAG GACTGAGTTA TCGAAGCTAA ACCACAAGGT CTTGGAATTT 
GGCTGGCCGG ACTTTCACGC ACCAGCCCTG GACAAGATCT GTAGCATGTG CAAAGCGATT 
GACACGTGGC TCAATGGAGA CTCGCACAAT GTGGTGGTGC TTCACAACAA GGGGAACCGA 
GGTCGAACTG GGGTCGTGGT GGCTGCTTAC ATGCACTATA GCAACATATC AGCAAGTGCT 
GACCAGGCCT TGGACAGGTT CGCCATGAGA CGGTTCTACG AGGATAAAGC ACTTCCTGTG 
GGTCAGCCGT CACAAAAAAG GTATGTGCGA TACTTCAATG GCCTTCTGTC GGGGCACATC 
AAAATCAACA ACAAACCTCT CTTCCTGCAC CACGTCATCA TGCACGGTAT CCCCAACTTT 
GAGTCTAAAG GAGGCTGCCG CCCTTTCCTG AAGATCTACC AAGCGATGCA GCCAGTCTAC 
ACGTCAGGGA TATACAACGT GCAGGGAGAC AGTAACACTA GTATCTGCAT CACCATCGAA 
CCAGGCCTCC TCCTGAAGGG AGACATCCTG CTGAAGTGTT ACCACAAACG ATACAGAGGC 
CCGACCAGAG ACGTAGTGTT CCGGGTGCAG TTCCACACCT GTGCCATCCA CGACCTTGGC 
GTGGTGTTCG GGAAGAGCGA ACTGGACGAG ACCTTCAAAG ACGACAGGTT TCCTGAATAC 
GGGAAAGTGG AGTTTGTTTT CTCCTATGCA CCAGAAAAAA TTAGAGGCTT AGACCACCTG 
GAGAACGGCC CCAGTGTTTC CGTGGACTAC AACACCCAGG AAAACCTGAT TCGCTGTGAT 
TCATATGACA ACTTTAGCCA GCGCTCTGAA GATGCCGTGG ATGGAGAGCG TGAGGTCATT 
CACACTCAAG GTCCACTTGA TGGAAGCCTT TATGCCCGGG TCTGTAAGAA AGACTCCCTG 
GAGGGAGTTG TCGCCATCAA CGGTAATCCA GTCCATGAAA ATTCCTTAGC CAGCACAGAG 
AACCAGTTAC AACACACAAG CTCCTTGCTT CAAACCGCCG AACAGACCCT TCCTGTGATT 
GGCCACGCTT CTCTTCCTGC TGTTGACCAC ACACTCTCTG TGAGCAGTGA CTCGGGCAAC 
TCCACTGCAT CCATTAAAAC TGATCGTACC GACGAGCACA GCCAGCCAGT GCACAGCACT 
GTCAACCACA ACAATCCGGC AGTTCCCCAC CCGACACTTA GTCCCCAGGA GAAAAAAGAG 
CTTGATCAGC TCCTTAGTGG CCTCGAAGCA CCGACTCTTC AACGACAAGC CTACCTGGCT 
ACATCCACCA GTCCGGGAGG CGGTGTCCGA CACCTGGTCC CAGCACAAGT GCACGTTAAC 
GGAGGCCACC CAAGGCTAGT TCCTGTCCCC TCAACGGAAG AGCGTGAGAC GGACATTCTT 
GACGACGAGC TGCCAAATAG CCAAGAGGGA AACAGTGTGG ACAGCCTGGG TACACTGTCA 
TCCTTAGAGG GTCAAGCAAC CCCAGGGGAT CTCTATTACC AACAAGAGAA GTCCGTCAGC 
AGACAAAACG ACGGGCCATA TCTTGAGAGA GGCGTTTGTC GGGAAAAAAA AAACGAGATG 
CCCATGCATG GAGTTCATAC TCCGATGATG CAGGAAAGAC CTGTGGACTC TGTATCCCCA 
CAAGGGGGAT ATAACAAATA CCAGAACGGT GGTGGGATGT ACCACTCTGA ATCCTATGGG 
ATTCCACCTG CCAGCAGCCC GGAGGGAAAC CCTAAACTGA TGCCCAGGGC CCCAGAGAGA 
AGCACAAGTA GCCGGGAGGC TGTTCAAAGG GGCCTCAATG CCTGGCACCA GTATAGCCTC 
CCAGATGATC CATTTGGACC TCCCCTGCAG TCAACCCATA GCATGCCACA GTTCCCCACA 
GCAGCCTCAC AGCGGGACAT AGAGCAGTCC ATCGAGGCAC TGAATATGCT CATGCTTGAC 
CTGGACCCCA TTAACTCTCA CATGTCCAAG TCCCATAGTG CACCCTCCGG GGAGAACATC 
CTGAATTCAT CCCAGGCACC CTTCTCCCAG ACCCTCTCCA GACCCTCATA CCAAGGAGAC 
TCCGCCATCC ACAGATACAA TAACTCTGAA TCTGTCAACA ATCCCTCCCA TCAGCCCATA 
TGGTCAGCTG GAAGAGTGTC AGCAGTGCCT AACCAGAGCC CTATTATGGA TTCTCAAGCA 
CATTTGCCCC AGAGATCCAG CTCGAGCTGT CAACACCAGA AAACCACCCC AACACACACC 
CCAGAGCCCA ATCTCCATGC ACGACAAGGA GCCATCCATT ACCCCATTGA TCCCTCCTTT 
AATTTCAACC AGCATCTCCC AGTTAGGCCT GCAAACACTT TCCCAGGTGT TTCCCCCTCC 
CCAGACCTGC AGGGTTCGTC TCCATACCAG GGCTTCAGTG CCTCTTCCTC ACCCCTTCCT 
GCGCTCACTC CACAGCCAAA GAACACATCG TCTTCATCCC TGCCTCAAGA ACAAGATGTT 
GAGGAAGAGG CGCTGAACTT GGAAGGTCTG GTGGCTCATC GCATTGCCGG CGTTCGCTCC 
AGAGGAATGA CCCCAGAAGT GACACAGGAG AGCGGTCGAC GTAGGACCAC CAGTGAGGGA 
CAGTACCAGA GCAGCCACGA CAGCACACCA ATGGTTGATT CACCGGACTT TGCCCACAGT 
TTGACCCTCA ACCCTGGAGG CAGACCCAGA GAGGGCCAGA TTCACAGCTA CAGGGAGGCG 
TTGGAGGACA GCGAGGTGGA CCTTGTCATG CACAGTCCCA CCTTCAGAGG CGGTGGTGAG 
AATTCCCCTC AGACCCCCAA GTTCCCTGTG TCACCACAGA CTCCTTACTT TAACATGTCT 
CGTGTCCCTC CAGGTCTGGC CAAGACTCCT CTGTCAGCAC TGGAACTGAA GCCCCATGAT 
CACGACTCTG ATTCAAATGA GGGAGAGCAA GATAACCACA CTTCCAGGGA CTCAAGAGTT 
CAACCCTTCA ACACCTCTTT CTCTTCCACC TACCCTAGTC ACAGCAGTGA TGGAAGAAGA 
ACCGGTTCTT CAGACGTTCA CAGCCACACC CCCTCCTACG CAAACAGACC CACCTCCGCT 
GAGGGCTCCC AGGTCAACGT CATGGGAGTC CACACAGTGC CCGGCAGTCC CAACACCCTC 
CATCGCACCG TAGCCACCAA CACTCCACCA AGCCCGGCCC TTCAGAGACG TCTGGGTCAA 
GCCAGTCCCT CACTAGCCAG ACACCAACCC CCAGCTGGTG CCAACAGGGA CAGTGTCCCC 
TCCAGTCCTC TGATTGGCCG CAACCCTAAA ACAGCAGCTG CTGGCGTGCC CCCCAGCCCT 
CTAATGGGCC GCCGAACGGC GATGAGCGGC CACAGCACGC CTGATGACAT CGGCCCACCA 
CCTTCTACGC CTGCCTTCCC TGTGTCCCCA CAGCTACCAG AAAAGAGGCA CATGTCCACT 
GGAGACGTGG AGAGGACAGA TAACAAGAAC CTTATGGCCG GTGTTGGCAG CAGTGCGCCA 
AACCTGTCTG GCACGCACAC GTTGACAGAC GTCCAAAAGT CCATCTATGA TGGGTATCCG 
GACATTAAGA TGAATGTGAA GTTTGTCCAA GACACATCCA AGTTCTGGTA CAAGCCAGAC 
ATCTCCAGAG AGCAAGCCAT CAATCTGTTG AAGGACAGAG AGCCCGGGGC CTTCATCATA 
AGGGACAGCC ATTCCTTCCG TGGAGCTTAC GGCCTTGCAA TGAAGGTGGC CTGCCCGCCG 
CCAACTGTCC AGCAGAACAA AAAAGTTGGA GACTTGACCA ACGAGCTTGT GAGACACTTC 
CTGATTGAGA CAAGCTCCAG AGGTGTTCGA CTCAAAGGCT GTCCCAATGA ACCCTACTTT 
GGTTGCCTCT CAGCGCTGGT TTATCAACAC TCAATGACCC CGCTAGCTCT GCCCTGTAAG 
CTGATGATCC CCACCAAAGA CCCGAATGAG GAAGCGCTGG AGTTGGCCAC ACCCACTGAC 
CCAGTTGTCG AGCTTCTGAA GCAAGGAGCA GTTCAGAAGG TTCCAGAGGA GGCTTACGCG 
TGTAACGTCC TCTACATCAA CTCGGTGGAC ATGGAGTCTC TCACGGGGCC CCAGGCCATC 
GCCAAGGCGA TCAGCCTGAC TTTGGCGACA AACCCCCTGC CGGCCGCCAC CACCGTCCAC 
TTCAAAGTGT CTATGCAGGG CATCACGCTC ACCGACAGCC AGAGGAAACT TTTTTTCAGA 
CGCCACTATC CCATCAACAC CATAACGTTC TGTGACATTG ACCCGCAGAA CAGGAAATGG 
GGAAAAGAAG GAGGCGGTTC AGTGAAGCTG TTTGGTTTTG TGGCACGGAA ACAAGGAAGC 
ACAACCGACA ACGTGAGCCA CCTGTTCGCA GAGCTGGACC CAGATCAGCC GGCTTCCGCC 
ATCACCAGTT TTGTCTCCAA GACGATGAAG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604679.1
CDS	69..5084
Translation	MPSVSLSLPSTLAGRTRTWVCLSCMFWPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELPPTPQLPLKHVDTPGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAGVRSRGMTPEVTQESGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREALEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_011606377.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes tensin 1 (tns1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606377.1

ORF Insert Sequence:

ATGCCCTCGG TCTCTCTGTC GTTGCCCTCC ACCCTGGCTG GGAGGACCAG GACCTGGGTT 
TGCCTCTCCT GCATGTTCTG GCCGGATGAG CTGGAGGGCG TCCACACTCA CACCTTCAAG 
CTGAAGCCAT TCAAGAAGGC CAAAAGCTGT GACATCTGTA AGCAGGCCAT CACCAAGGAG 
GGCCTCGTCT GCAAAGCGTG TCGGCTCTCC TGCCACAAAA AATGTGAAGT GAAGGTCACC 
ACATCATGTC AGACGTCCAC CAACCATGAG CTGCCCCCCA CTCCGCAGCT GCCATTAAAG 
CATGTAGACA CGCCGGGATC TGCGAGGTCC TGCAAGAGTG TGGAGATAAG GCGGAAGCAG 
TCTCGAAGCC AGAGTGTGGT TCTGACCATG GAGGAGAACT ATGAGGTAGA CCTGGTCTAC 
ATCACGGAGA GGATCATCTC CCTCTGCTTC CCCATTGGCA TGGAGGACCA GGGCTACATC 
GCCAACCTCA AGGAGGTGGC CACCATGCTG CGATCCAAAC ATGGAGATCA TTATTTGGTG 
CTGAACCTGA GTGAATGGAG GACTGAGTTA TCGAAGCTAA ACCACAAGGT CTTGGAATTT 
GGCTGGCCGG ACTTTCACGC ACCAGCCCTG GACAAGATCT GTAGCATGTG CAAAGCGATT 
GACACGTGGC TCAATGGAGA CTCGCACAAT GTGGTGGTGC TTCACAACAA GGGGAACCGA 
GGTCGAACTG GGGTCGTGGT GGCTGCTTAC ATGCACTATA GCAACATATC AGCAAGTGCT 
GACCAGGCCT TGGACAGGTT CGCCATGAGA CGGTTCTACG AGGATAAAGC ACTTCCTGTG 
GGTCAGCCGT CACAAAAAAG GTATGTGCGA TACTTCAATG GCCTTCTGTC GGGGCACATC 
AAAATCAACA ACAAACCTCT CTTCCTGCAC CACGTCATCA TGCACGGTAT CCCCAACTTT 
GAGTCTAAAG GAGGCTGCCG CCCTTTCCTG AAGATCTACC AAGCGATGCA GCCAGTCTAC 
ACGTCAGGGA TATACAACGT GCAGGGAGAC AGTAACACTA GTATCTGCAT CACCATCGAA 
CCAGGCCTCC TCCTGAAGGG AGACATCCTG CTGAAGTGTT ACCACAAACG ATACAGAGGC 
CCGACCAGAG ACGTAGTGTT CCGGGTGCAG TTCCACACCT GTGCCATCCA CGACCTTGGC 
GTGGTGTTCG GGAAGAGCGA ACTGGACGAG ACCTTCAAAG ACGACAGGTT TCCTGAATAC 
GGGAAAGTGG AGTTTGTTTT CTCCTATGCA CCAGAAAAAA TTAGAGGCTT AGACCACCTG 
GAGAACGGCC CCAGTGTTTC CGTGGACTAC AACACCCAGG AAAACCTGAT TCGCTGTGAT 
TCATATGACA ACTTTAGCCA GCGCTCTGAA GATGCCGTGG ATGGAGAGCG TGAGGTCATT 
CACACTCAAG GTCCACTTGA TGGAAGCCTT TATGCCCGGG TCTGTAAGAA AGACTCCCTG 
GAGGGAGTTG TCGCCATCAA CGGTAATCCA GTCCATGAAA ATTCCTTAGC CAGCACAGAG 
AACCAGTTAC AACACACAAG CTCCTTGCTT CAAACCGCCG AACAGACCCT TCCTGTGATT 
GGCCACGCTT CTCTTCCTGC TGTTGACCAC ACACTCTCTG TGAGCAGTGA CTCGGGCAAC 
TCCACTGCAT CCATTAAAAC TGATCGTACC GACGAGCACA GCCAGCCAGT GCACAGCACT 
GTCAACCACA ACAATCCGGC AGTTCCCCAC CCGACACTTA GTCCCCAGGA GAAAAAAGAG 
CTTGATCAGC TCCTTAGTGG CCTCGAAGCA CCGACTCTTC AACGACAAGC CTACCTGGCT 
ACATCCACCA GTCCGGGAGG CGGTGTCCGA CACCTGGTCC CAGCACAAGT GCACGTTAAC 
GGAGGCCACC CAAGGCTAGT TCCTGTCCCC TCAACGGAAG AGCGTGAGAC GGACATTCTT 
GACGACGAGC TGCCAAATAG CCAAGAGGGA AACAGTGTGG ACAGCCTGGG TACACTGTCA 
TCCTTAGAGG GTCAAGCAAC CCCAGGGGAT CTCTATTACC AACAAGAGAA GTCCGTCAGC 
AGACAAAACG ACGGGCCATA TCTTGAGAGA GGCGTTTGTC GGGAAAAAAA AAACGAGATG 
CCCATGCATG GAGTTCATAC TCCGATGATG CAGGAAAGAC CTGTGGACTC TGTATCCCCA 
CAAGGGGGAT ATAACAAATA CCAGAACGGT GGTGGGATGT ACCACTCTGA ATCCTATGGG 
ATTCCACCTG CCAGCAGCCC GGAGGGAAAC CCTAAACTGA TGCCCAGGGC CCCAGAGAGA 
AGCACAAGTA GCCGGGAGGC TGTTCAAAGG GGCCTCAATG CCTGGCACCA GTATAGCCTC 
CCAGATGATC CATTTGGACC TCCCCTGCAG TCAACCCATA GCATGCCACA GTTCCCCACA 
GCAGCCTCAC AGCGGGACAT AGAGCAGTCC ATCGAGGCAC TGAATATGCT CATGCTTGAC 
CTGGACCCCA TTAACTCTCA CATGTCCAAG TCCCATAGTG CACCCTCCGG GGAGAACATC 
CTGAATTCAT CCCAGGCACC CTTCTCCCAG ACCCTCTCCA GACCCTCATA CCAAGGAGAC 
TCCGCCATCC ACAGATACAA TAACTCTGAA TCTGTCAACA ATCCCTCCCA TCAGCCCATA 
TGGTCAGCTG GAAGAGTGTC AGCAGTGCCT AACCAGAGCC CTATTATGGA TTCTCAAGCA 
CATTTGCCCC AGAGATCCAG CTCGAGCTGT CAACACCAGA AAACCACCCC AACACACACC 
CCAGAGCCCA ATCTCCATGC ACGACAAGGA GCCATCCATT ACCCCATTGA TCCCTCCTTT 
AATTTCAACC AGCATCTCCC AGTTAGGCCT GCAAACACTT TCCCAGGTGT TTCCCCCTCC 
CCAGACCTGC AGGGTTCGTC TCCATACCAG GGCTTCAGTG CCTCTTCCTC ACCCCTTCCT 
GCGCTCACTC CACAGCCAAA GAACACATCG TCTTCATCCC TGCCTCAAGA ACAAGATGTT 
GAGGAAGAGG CGCTGAACTT GGAAGGTCTG GTGGCTCATC GCATTGCCGG CGTTCGCTCC 
AGAGGAATGA CCCCAGAAGT GACACAGGAG AGCGGTCGAC GTAGGACCAC CAGTGAGGGA 
CAGTACCAGA GCAGCCACGA CAGCACACCA ATGGTTGATT CACCGGACTT TGCCCACAGT 
TTGACCCTCA ACCCTGGAGG CAGACCCAGA GAGGGCCAGA TTCACAGCTA CAGGGAGGCG 
TTGGAGGACA GCGAGGTGGA CCTTGTCATG CACAGTCCCA CCTTCAGAGG CGGTGGTGAG 
AATTCCCCTC AGACCCCCAA GTTCCCTGTG TCACCACAGA CTCCTTACTT TAACATGTCT 
CGTGTCCCTC CAGGTCTGGC CAAGACTCCT CTGTCAGCAC TGGAACTGAA GCCCCATGAT 
CACGACTCTG ATTCAAATGA GGGAGAGCAA GATAACCACA CTTCCAGGGA CTCAAGAGTT 
CAACCCTTCA ACACCTCTTT CTCTTCCACC TACCCTAGTC ACAGCAGTGA TGGAAGAAGA 
ACCGGTTCTT CAGACGTTCA CAGCCACACC CCCTCCTACG CAAACAGACC CACCTCCGCT 
GAGGGCTCCC AGGTCAACGT CATGGGAGTC CACACAGTGC CCGGCAGTCC CAACACCCTC 
CATCGCACCG TAGCCACCAA CACTCCACCA AGCCCGGCCC TTCAGAGACG TCTGGGTCAA 
GCCAGTCCCT CACTAGCCAG ACACCAACCC CCAGCTGGTG CCAACAGGGA CAGTGTCCCC 
TCCAGTCCTC TGATTGGCCG CAACCCTAAA ACAGCAGCTG CTGGCGTGCC CCCCAGCCCT 
CTAATGGGCC GCCGAACGGC GATGAGCGGC CACAGCACGC CTGATGACAT CGGCCCACCA 
CCTTCTACGC CTGCCTTCCC TGTGTCCCCA CAGCTACCAG AAAAGAGGCA CATGTCCACT 
GGAGACGTGG AGAGGACAGA TAACAAGAAC CTTATGGCCG GTGTTGGCAG CAGTGCGCCA 
AACCTGTCTG GCACGCACAC GTTGACAGAC GTCCAAAAGT CCATCTATGA TGGGTATCCG 
GACATTAAGA TGAATGTGAA GTTTGTCCAA GACACATCCA AGTTCTGGTA CAAGCCAGAC 
ATCTCCAGAG AGCAAGCCAT CAATCTGTTG AAGGACAGAG AGCCCGGGGC CTTCATCATA 
AGGGACAGCC ATTCCTTCCG TGGAGCTTAC GGCCTTGCAA TGAAGGTGGC CTGCCCGCCG 
CCAACTGTCC AGCAGAACAA AAAAGTTGGA GACTTGACCA ACGAGCTTGT GAGACACTTC 
CTGATTGAGA CAAGCTCCAG AGGTGTTCGA CTCAAAGGCT GTCCCAATGA ACCCTACTTT 
GGTTGCCTCT CAGCGCTGGT TTATCAACAC TCAATGACCC CGCTAGCTCT GCCCTGTAAG 
CTGATGATCC CCACCAAAGA CCCGAATGAG GAAGCGCTGG AGTTGGCCAC ACCCACTGAC 
CCAGTTGTCG AGCTTCTGAA GCAAGGAGCA GTTCAGAAGG TTCCAGAGGA GGCTTACGCG 
TGTAACGTCC TCTACATCAA CTCGGTGGAC ATGGAGTCTC TCACGGGGCC CCAGGCCATC 
GCCAAGGCGA TCAGCCTGAC TTTGGCGACA AACCCCCTGC CGGCCGCCAC CACCGTCCAC 
TTCAAAGTGT CTATGCAGGG CATCACGCTC ACCGACAGCC AGAGGAAACT TTTTTTCAGA 
CGCCACTATC CCATCAACAC CATAACGTTC TGTGACATTG ACCCGCAGAA CAGGAAATGG 
GGAAAAGAAG GAGGCGGTTC AGTGAAGCTG TTTGGTTTTG TGGCACGGAA ACAAGGAAGC 
ACAACCGACA ACGTGAGCCA CCTGTTCGCA GAGCTGGACC CAGATCAGCC GGCTTCCGCC 
ATCACCAGTT TTGTCTCCAA GACGATGAAG CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_011606378.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604680.1 Takifugu rubripes tensin 1 (tns1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo20982
Clone ID Related Accession (Same CDS sequence)	XM_011606378.1
Accession Version	XM_011606378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	tensin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070998.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG TCTCTCTGTC GTTGCCCTCC ACCCTGGCTG GGAGGACCAG GACCTGGGTT 
TGCCTCTCCT GCATGTTCTG GCCGGATGAG CTGGAGGGCG TCCACACTCA CACCTTCAAG 
CTGAAGCCAT TCAAGAAGGC CAAAAGCTGT GACATCTGTA AGCAGGCCAT CACCAAGGAG 
GGCCTCGTCT GCAAAGCGTG TCGGCTCTCC TGCCACAAAA AATGTGAAGT GAAGGTCACC 
ACATCATGTC AGACGTCCAC CAACCATGAG CTGCCCCCCA CTCCGCAGCT GCCATTAAAG 
CATGTAGACA CGCCGGGATC TGCGAGGTCC TGCAAGAGTG TGGAGATAAG GCGGAAGCAG 
TCTCGAAGCC AGAGTGTGGT TCTGACCATG GAGGAGAACT ATGAGGTAGA CCTGGTCTAC 
ATCACGGAGA GGATCATCTC CCTCTGCTTC CCCATTGGCA TGGAGGACCA GGGCTACATC 
GCCAACCTCA AGGAGGTGGC CACCATGCTG CGATCCAAAC ATGGAGATCA TTATTTGGTG 
CTGAACCTGA GTGAATGGAG GACTGAGTTA TCGAAGCTAA ACCACAAGGT CTTGGAATTT 
GGCTGGCCGG ACTTTCACGC ACCAGCCCTG GACAAGATCT GTAGCATGTG CAAAGCGATT 
GACACGTGGC TCAATGGAGA CTCGCACAAT GTGGTGGTGC TTCACAACAA GGGGAACCGA 
GGTCGAACTG GGGTCGTGGT GGCTGCTTAC ATGCACTATA GCAACATATC AGCAAGTGCT 
GACCAGGCCT TGGACAGGTT CGCCATGAGA CGGTTCTACG AGGATAAAGC ACTTCCTGTG 
GGTCAGCCGT CACAAAAAAG GTATGTGCGA TACTTCAATG GCCTTCTGTC GGGGCACATC 
AAAATCAACA ACAAACCTCT CTTCCTGCAC CACGTCATCA TGCACGGTAT CCCCAACTTT 
GAGTCTAAAG GAGGCTGCCG CCCTTTCCTG AAGATCTACC AAGCGATGCA GCCAGTCTAC 
ACGTCAGGGA TATACAACGT GCAGGGAGAC AGTAACACTA GTATCTGCAT CACCATCGAA 
CCAGGCCTCC TCCTGAAGGG AGACATCCTG CTGAAGTGTT ACCACAAACG ATACAGAGGC 
CCGACCAGAG ACGTAGTGTT CCGGGTGCAG TTCCACACCT GTGCCATCCA CGACCTTGGC 
GTGGTGTTCG GGAAGAGCGA ACTGGACGAG ACCTTCAAAG ACGACAGGTT TCCTGAATAC 
GGGAAAGTGG AGTTTGTTTT CTCCTATGCA CCAGAAAAAA TTAGAGGCTT AGACCACCTG 
GAGAACGGCC CCAGTGTTTC CGTGGACTAC AACACCCAGG AAAACCTGAT TCGCTGTGAT 
TCATATGACA ACTTTAGCCA GCGCTCTGAA GATGCCGTGG ATGGAGAGCG TGAGGTCATT 
CACACTCAAG GTCCACTTGA TGGAAGCCTT TATGCCCGGG TCTGTAAGAA AGACTCCCTG 
GAGGGAGTTG TCGCCATCAA CGGTAATCCA GTCCATGAAA ATTCCTTAGC CAGCACAGAG 
AACCAGTTAC AACACACAAG CTCCTTGCTT CAAACCGCCG AACAGACCCT TCCTGTGATT 
GGCCACGCTT CTCTTCCTGC TGTTGACCAC ACACTCTCTG TGAGCAGTGA CTCGGGCAAC 
TCCACTGCAT CCATTAAAAC TGATCGTACC GACGAGCACA GCCAGCCAGT GCACAGCACT 
GTCAACCACA ACAATCCGGC AGTTCCCCAC CCGACACTTA GTCCCCAGGA GAAAAAAGAG 
CTTGATCAGC TCCTTAGTGG CCTCGAAGCA CCGACTCTTC AACGACAAGC CTACCTGGCT 
ACATCCACCA GTCCGGGAGG CGGTGTCCGA CACCTGGTCC CAGCACAAGT GCACGTTAAC 
GGAGGCCACC CAAGGCTAGT TCCTGTCCCC TCAACGGAAG AGCGTGAGAC GGACATTCTT 
GACGACGAGC TGCCAAATAG CCAAGAGGGA AACAGTGTGG ACAGCCTGGG TACACTGTCA 
TCCTTAGAGG GTCAAGCAAC CCCAGGGGAT CTCTATTACC AACAAGAGAA GTCCGTCAGC 
AGACAAAACG ACGGGCCATA TCTTGAGAGA GGCGTTTGTC GGGAAAAAAA AAACGAGATG 
CCCATGCATG GAGTTCATAC TCCGATGATG CAGGAAAGAC CTGTGGACTC TGTATCCCCA 
CAAGGGGGAT ATAACAAATA CCAGAACGGT GGTGGGATGT ACCACTCTGA ATCCTATGGG 
ATTCCACCTG CCAGCAGCCC GGAGGGAAAC CCTAAACTGA TGCCCAGGGC CCCAGAGAGA 
AGCACAAGTA GCCGGGAGGC TGTTCAAAGG GGCCTCAATG CCTGGCACCA GTATAGCCTC 
CCAGATGATC CATTTGGACC TCCCCTGCAG TCAACCCATA GCATGCCACA GTTCCCCACA 
GCAGCCTCAC AGCGGGACAT AGAGCAGTCC ATCGAGGCAC TGAATATGCT CATGCTTGAC 
CTGGACCCCA TTAACTCTCA CATGTCCAAG TCCCATAGTG CACCCTCCGG GGAGAACATC 
CTGAATTCAT CCCAGGCACC CTTCTCCCAG ACCCTCTCCA GACCCTCATA CCAAGGAGAC 
TCCGCCATCC ACAGATACAA TAACTCTGAA TCTGTCAACA ATCCCTCCCA TCAGCCCATA 
TGGTCAGCTG GAAGAGTGTC AGCAGTGCCT AACCAGAGCC CTATTATGGA TTCTCAAGCA 
CATTTGCCCC AGAGATCCAG CTCGAGCTGT CAACACCAGA AAACCACCCC AACACACACC 
CCAGAGCCCA ATCTCCATGC ACGACAAGGA GCCATCCATT ACCCCATTGA TCCCTCCTTT 
AATTTCAACC AGCATCTCCC AGTTAGGCCT GCAAACACTT TCCCAGGTGT TTCCCCCTCC 
CCAGACCTGC AGGGTTCGTC TCCATACCAG GGCTTCAGTG CCTCTTCCTC ACCCCTTCCT 
GCGCTCACTC CACAGCCAAA GAACACATCG TCTTCATCCC TGCCTCAAGA ACAAGATGTT 
GAGGAAGAGG CGCTGAACTT GGAAGGTCTG GTGGCTCATC GCATTGCCGG CGTTCGCTCC 
AGAGGAATGA CCCCAGAAGT GACACAGGAG AGCGGTCGAC GTAGGACCAC CAGTGAGGGA 
CAGTACCAGA GCAGCCACGA CAGCACACCA ATGGTTGATT CACCGGACTT TGCCCACAGT 
TTGACCCTCA ACCCTGGAGG CAGACCCAGA GAGGGCCAGA TTCACAGCTA CAGGGAGGCG 
TTGGAGGACA GCGAGGTGGA CCTTGTCATG CACAGTCCCA CCTTCAGAGG CGGTGGTGAG 
AATTCCCCTC AGACCCCCAA GTTCCCTGTG TCACCACAGA CTCCTTACTT TAACATGTCT 
CGTGTCCCTC CAGGTCTGGC CAAGACTCCT CTGTCAGCAC TGGAACTGAA GCCCCATGAT 
CACGATAACC ACACTTCCAG GGACTCAAGA GTTCAACCCT TCAACACCTC TTTCTCTTCC 
ACCTACCCTA GTCACAGCAG TGATGGAAGA AGAACCGGTT CTTCAGACGT TCACAGCCAC 
ACCCCCTCCT ACGCAAACAG ACCCACCTCC GCTGAGGGCT CCCAGGTCAA CGTCATGGGA 
GTCCACACAG TGCCCGGCAG TCCCAACACC CTCCATCGCA CCGTAGCCAC CAACACTCCA 
CCAAGCCCGG CCCTTCAGAG ACGTCTGGGT CAAGCCAGTC CCTCACTAGC CAGACACCAA 
CCCCCAGCTG GTGCCAACAG GGACAGTGTC CCCTCCAGTC CTCTGATTGG CCGCAACCCT 
AAAACAGCAG CTGCTGGCGT GCCCCCCAGC CCTCTAATGG GCCGCCGAAC GGCGATGAGC 
GGCCACAGCA CGCCTGATGA CATCGGCCCA CCACCTTCTA CGCCTGCCTT CCCTGTGTCC 
CCACAGCTAC CAGAAAAGAG GCACATGTCC ACTGGAGACG TGGAGAGGAC AGATAACAAG 
AACCTTATGG CCGGTGTTGG CAGCAGTGCG CCAAACCTGT CTGGCACGCA CACGTTGACA 
GACGTCCAAA AGTCCATCTA TGATGGGTAT CCGGACATTA AGATGAATGT GAAGTTTGTC 
CAAGACACAT CCAAGTTCTG GTACAAGCCA GACATCTCCA GAGAGCAAGC CATCAATCTG 
TTGAAGGACA GAGAGCCCGG GGCCTTCATC ATAAGGGACA GCCATTCCTT CCGTGGAGCT 
TACGGCCTTG CAATGAAGGT GGCCTGCCCG CCGCCAACTG TCCAGCAGAA CAAAAAAGTT 
GGAGACTTGA CCAACGAGCT TGTGAGACAC TTCCTGATTG AGACAAGCTC CAGAGGTGTT 
CGACTCAAAG GCTGTCCCAA TGAACCCTAC TTTGGTTGCC TCTCAGCGCT GGTTTATCAA 
CACTCAATGA CCCCGCTAGC TCTGCCCTGT AAGCTGATGA TCCCCACCAA AGACCCGAAT 
GAGGAAGCGC TGGAGTTGGC CACACCCACT GACCCAGTTG TCGAGCTTCT GAAGCAAGGA 
GCAGTTCAGA AGGTTCCAGA GGAGGCTTAC GCGTGTAACG TCCTCTACAT CAACTCGGTG 
GACATGGAGT CTCTCACGGG GCCCCAGGCC ATCGCCAAGG CGATCAGCCT GACTTTGGCG 
ACAAACCCCC TGCCGGCCGC CACCACCGTC CACTTCAAAG TGTCTATGCA GGGCATCACG 
CTCACCGACA GCCAGAGGAA ACTTTTTTTC AGACGCCACT ATCCCATCAA CACCATAACG 
TTCTGTGACA TTGACCCGCA GAACAGGAAA TGGGGAAAAG AAGGAGGCGG TTCAGTGAAG 
CTGTTTGGTT TTGTGGCACG GAAACAAGGA AGCACAACCG ACAACGTGAG CCACCTGTTC 
GCAGAGCTGG ACCCAGATCA GCCGGCTTCC GCCATCACCA GTTTTGTCTC CAAGACGATG 
AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604680.1
CDS	69..5057
Translation	MPSVSLSLPSTLAGRTRTWVCLSCMFWPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELPPTPQLPLKHVDTPGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAGVRSRGMTPEVTQESGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREALEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_011606378.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes tensin 1 (tns1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606378.1

ORF Insert Sequence:

ATGCCCTCGG TCTCTCTGTC GTTGCCCTCC ACCCTGGCTG GGAGGACCAG GACCTGGGTT 
TGCCTCTCCT GCATGTTCTG GCCGGATGAG CTGGAGGGCG TCCACACTCA CACCTTCAAG 
CTGAAGCCAT TCAAGAAGGC CAAAAGCTGT GACATCTGTA AGCAGGCCAT CACCAAGGAG 
GGCCTCGTCT GCAAAGCGTG TCGGCTCTCC TGCCACAAAA AATGTGAAGT GAAGGTCACC 
ACATCATGTC AGACGTCCAC CAACCATGAG CTGCCCCCCA CTCCGCAGCT GCCATTAAAG 
CATGTAGACA CGCCGGGATC TGCGAGGTCC TGCAAGAGTG TGGAGATAAG GCGGAAGCAG 
TCTCGAAGCC AGAGTGTGGT TCTGACCATG GAGGAGAACT ATGAGGTAGA CCTGGTCTAC 
ATCACGGAGA GGATCATCTC CCTCTGCTTC CCCATTGGCA TGGAGGACCA GGGCTACATC 
GCCAACCTCA AGGAGGTGGC CACCATGCTG CGATCCAAAC ATGGAGATCA TTATTTGGTG 
CTGAACCTGA GTGAATGGAG GACTGAGTTA TCGAAGCTAA ACCACAAGGT CTTGGAATTT 
GGCTGGCCGG ACTTTCACGC ACCAGCCCTG GACAAGATCT GTAGCATGTG CAAAGCGATT 
GACACGTGGC TCAATGGAGA CTCGCACAAT GTGGTGGTGC TTCACAACAA GGGGAACCGA 
GGTCGAACTG GGGTCGTGGT GGCTGCTTAC ATGCACTATA GCAACATATC AGCAAGTGCT 
GACCAGGCCT TGGACAGGTT CGCCATGAGA CGGTTCTACG AGGATAAAGC ACTTCCTGTG 
GGTCAGCCGT CACAAAAAAG GTATGTGCGA TACTTCAATG GCCTTCTGTC GGGGCACATC 
AAAATCAACA ACAAACCTCT CTTCCTGCAC CACGTCATCA TGCACGGTAT CCCCAACTTT 
GAGTCTAAAG GAGGCTGCCG CCCTTTCCTG AAGATCTACC AAGCGATGCA GCCAGTCTAC 
ACGTCAGGGA TATACAACGT GCAGGGAGAC AGTAACACTA GTATCTGCAT CACCATCGAA 
CCAGGCCTCC TCCTGAAGGG AGACATCCTG CTGAAGTGTT ACCACAAACG ATACAGAGGC 
CCGACCAGAG ACGTAGTGTT CCGGGTGCAG TTCCACACCT GTGCCATCCA CGACCTTGGC 
GTGGTGTTCG GGAAGAGCGA ACTGGACGAG ACCTTCAAAG ACGACAGGTT TCCTGAATAC 
GGGAAAGTGG AGTTTGTTTT CTCCTATGCA CCAGAAAAAA TTAGAGGCTT AGACCACCTG 
GAGAACGGCC CCAGTGTTTC CGTGGACTAC AACACCCAGG AAAACCTGAT TCGCTGTGAT 
TCATATGACA ACTTTAGCCA GCGCTCTGAA GATGCCGTGG ATGGAGAGCG TGAGGTCATT 
CACACTCAAG GTCCACTTGA TGGAAGCCTT TATGCCCGGG TCTGTAAGAA AGACTCCCTG 
GAGGGAGTTG TCGCCATCAA CGGTAATCCA GTCCATGAAA ATTCCTTAGC CAGCACAGAG 
AACCAGTTAC AACACACAAG CTCCTTGCTT CAAACCGCCG AACAGACCCT TCCTGTGATT 
GGCCACGCTT CTCTTCCTGC TGTTGACCAC ACACTCTCTG TGAGCAGTGA CTCGGGCAAC 
TCCACTGCAT CCATTAAAAC TGATCGTACC GACGAGCACA GCCAGCCAGT GCACAGCACT 
GTCAACCACA ACAATCCGGC AGTTCCCCAC CCGACACTTA GTCCCCAGGA GAAAAAAGAG 
CTTGATCAGC TCCTTAGTGG CCTCGAAGCA CCGACTCTTC AACGACAAGC CTACCTGGCT 
ACATCCACCA GTCCGGGAGG CGGTGTCCGA CACCTGGTCC CAGCACAAGT GCACGTTAAC 
GGAGGCCACC CAAGGCTAGT TCCTGTCCCC TCAACGGAAG AGCGTGAGAC GGACATTCTT 
GACGACGAGC TGCCAAATAG CCAAGAGGGA AACAGTGTGG ACAGCCTGGG TACACTGTCA 
TCCTTAGAGG GTCAAGCAAC CCCAGGGGAT CTCTATTACC AACAAGAGAA GTCCGTCAGC 
AGACAAAACG ACGGGCCATA TCTTGAGAGA GGCGTTTGTC GGGAAAAAAA AAACGAGATG 
CCCATGCATG GAGTTCATAC TCCGATGATG CAGGAAAGAC CTGTGGACTC TGTATCCCCA 
CAAGGGGGAT ATAACAAATA CCAGAACGGT GGTGGGATGT ACCACTCTGA ATCCTATGGG 
ATTCCACCTG CCAGCAGCCC GGAGGGAAAC CCTAAACTGA TGCCCAGGGC CCCAGAGAGA 
AGCACAAGTA GCCGGGAGGC TGTTCAAAGG GGCCTCAATG CCTGGCACCA GTATAGCCTC 
CCAGATGATC CATTTGGACC TCCCCTGCAG TCAACCCATA GCATGCCACA GTTCCCCACA 
GCAGCCTCAC AGCGGGACAT AGAGCAGTCC ATCGAGGCAC TGAATATGCT CATGCTTGAC 
CTGGACCCCA TTAACTCTCA CATGTCCAAG TCCCATAGTG CACCCTCCGG GGAGAACATC 
CTGAATTCAT CCCAGGCACC CTTCTCCCAG ACCCTCTCCA GACCCTCATA CCAAGGAGAC 
TCCGCCATCC ACAGATACAA TAACTCTGAA TCTGTCAACA ATCCCTCCCA TCAGCCCATA 
TGGTCAGCTG GAAGAGTGTC AGCAGTGCCT AACCAGAGCC CTATTATGGA TTCTCAAGCA 
CATTTGCCCC AGAGATCCAG CTCGAGCTGT CAACACCAGA AAACCACCCC AACACACACC 
CCAGAGCCCA ATCTCCATGC ACGACAAGGA GCCATCCATT ACCCCATTGA TCCCTCCTTT 
AATTTCAACC AGCATCTCCC AGTTAGGCCT GCAAACACTT TCCCAGGTGT TTCCCCCTCC 
CCAGACCTGC AGGGTTCGTC TCCATACCAG GGCTTCAGTG CCTCTTCCTC ACCCCTTCCT 
GCGCTCACTC CACAGCCAAA GAACACATCG TCTTCATCCC TGCCTCAAGA ACAAGATGTT 
GAGGAAGAGG CGCTGAACTT GGAAGGTCTG GTGGCTCATC GCATTGCCGG CGTTCGCTCC 
AGAGGAATGA CCCCAGAAGT GACACAGGAG AGCGGTCGAC GTAGGACCAC CAGTGAGGGA 
CAGTACCAGA GCAGCCACGA CAGCACACCA ATGGTTGATT CACCGGACTT TGCCCACAGT 
TTGACCCTCA ACCCTGGAGG CAGACCCAGA GAGGGCCAGA TTCACAGCTA CAGGGAGGCG 
TTGGAGGACA GCGAGGTGGA CCTTGTCATG CACAGTCCCA CCTTCAGAGG CGGTGGTGAG 
AATTCCCCTC AGACCCCCAA GTTCCCTGTG TCACCACAGA CTCCTTACTT TAACATGTCT 
CGTGTCCCTC CAGGTCTGGC CAAGACTCCT CTGTCAGCAC TGGAACTGAA GCCCCATGAT 
CACGATAACC ACACTTCCAG GGACTCAAGA GTTCAACCCT TCAACACCTC TTTCTCTTCC 
ACCTACCCTA GTCACAGCAG TGATGGAAGA AGAACCGGTT CTTCAGACGT TCACAGCCAC 
ACCCCCTCCT ACGCAAACAG ACCCACCTCC GCTGAGGGCT CCCAGGTCAA CGTCATGGGA 
GTCCACACAG TGCCCGGCAG TCCCAACACC CTCCATCGCA CCGTAGCCAC CAACACTCCA 
CCAAGCCCGG CCCTTCAGAG ACGTCTGGGT CAAGCCAGTC CCTCACTAGC CAGACACCAA 
CCCCCAGCTG GTGCCAACAG GGACAGTGTC CCCTCCAGTC CTCTGATTGG CCGCAACCCT 
AAAACAGCAG CTGCTGGCGT GCCCCCCAGC CCTCTAATGG GCCGCCGAAC GGCGATGAGC 
GGCCACAGCA CGCCTGATGA CATCGGCCCA CCACCTTCTA CGCCTGCCTT CCCTGTGTCC 
CCACAGCTAC CAGAAAAGAG GCACATGTCC ACTGGAGACG TGGAGAGGAC AGATAACAAG 
AACCTTATGG CCGGTGTTGG CAGCAGTGCG CCAAACCTGT CTGGCACGCA CACGTTGACA 
GACGTCCAAA AGTCCATCTA TGATGGGTAT CCGGACATTA AGATGAATGT GAAGTTTGTC 
CAAGACACAT CCAAGTTCTG GTACAAGCCA GACATCTCCA GAGAGCAAGC CATCAATCTG 
TTGAAGGACA GAGAGCCCGG GGCCTTCATC ATAAGGGACA GCCATTCCTT CCGTGGAGCT 
TACGGCCTTG CAATGAAGGT GGCCTGCCCG CCGCCAACTG TCCAGCAGAA CAAAAAAGTT 
GGAGACTTGA CCAACGAGCT TGTGAGACAC TTCCTGATTG AGACAAGCTC CAGAGGTGTT 
CGACTCAAAG GCTGTCCCAA TGAACCCTAC TTTGGTTGCC TCTCAGCGCT GGTTTATCAA 
CACTCAATGA CCCCGCTAGC TCTGCCCTGT AAGCTGATGA TCCCCACCAA AGACCCGAAT 
GAGGAAGCGC TGGAGTTGGC CACACCCACT GACCCAGTTG TCGAGCTTCT GAAGCAAGGA 
GCAGTTCAGA AGGTTCCAGA GGAGGCTTAC GCGTGTAACG TCCTCTACAT CAACTCGGTG 
GACATGGAGT CTCTCACGGG GCCCCAGGCC ATCGCCAAGG CGATCAGCCT GACTTTGGCG 
ACAAACCCCC TGCCGGCCGC CACCACCGTC CACTTCAAAG TGTCTATGCA GGGCATCACG 
CTCACCGACA GCCAGAGGAA ACTTTTTTTC AGACGCCACT ATCCCATCAA CACCATAACG 
TTCTGTGACA TTGACCCGCA GAACAGGAAA TGGGGAAAAG AAGGAGGCGG TTCAGTGAAG 
CTGTTTGGTT TTGTGGCACG GAAACAAGGA AGCACAACCG ACAACGTGAG CCACCTGTTC 
GCAGAGCTGG ACCCAGATCA GCCGGCTTCC GCCATCACCA GTTTTGTCTC CAAGACGATG 
AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_011606379.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604681.1 Takifugu rubripes tensin 1 (tns1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo20983
Clone ID Related Accession (Same CDS sequence)	XM_011606379.1
Accession Version	XM_011606379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	tensin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070998.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG TCTCTCTGTC GTTGCCCTCC ACCCTGGCTG GGAGGACCAG GACCTGGGTT 
TGCCTCTCCT GCATGTTCTG GCCGGATGAG CTGGAGGGCG TCCACACTCA CACCTTCAAG 
CTGAAGCCAT TCAAGAAGGC CAAAAGCTGT GACATCTGTA AGCAGGCCAT CACCAAGGAG 
GGCCTCGTCT GCAAAGCGTG TCGGCTCTCC TGCCACAAAA AATGTGAAGT GAAGGTCACC 
ACATCATGTC AGACGTCCAC CAACCATGAG CTGGGATCTG CGAGGTCCTG CAAGAGTGTG 
GAGATAAGGC GGAAGCAGTC TCGAAGCCAG AGTGTGGTTC TGACCATGGA GGAGAACTAT 
GAGGTAGACC TGGTCTACAT CACGGAGAGG ATCATCTCCC TCTGCTTCCC CATTGGCATG 
GAGGACCAGG GCTACATCGC CAACCTCAAG GAGGTGGCCA CCATGCTGCG ATCCAAACAT 
GGAGATCATT ATTTGGTGCT GAACCTGAGT GAATGGAGGA CTGAGTTATC GAAGCTAAAC 
CACAAGGTCT TGGAATTTGG CTGGCCGGAC TTTCACGCAC CAGCCCTGGA CAAGATCTGT 
AGCATGTGCA AAGCGATTGA CACGTGGCTC AATGGAGACT CGCACAATGT GGTGGTGCTT 
CACAACAAGG GGAACCGAGG TCGAACTGGG GTCGTGGTGG CTGCTTACAT GCACTATAGC 
AACATATCAG CAAGTGCTGA CCAGGCCTTG GACAGGTTCG CCATGAGACG GTTCTACGAG 
GATAAAGCAC TTCCTGTGGG TCAGCCGTCA CAAAAAAGGT ATGTGCGATA CTTCAATGGC 
CTTCTGTCGG GGCACATCAA AATCAACAAC AAACCTCTCT TCCTGCACCA CGTCATCATG 
CACGGTATCC CCAACTTTGA GTCTAAAGGA GGCTGCCGCC CTTTCCTGAA GATCTACCAA 
GCGATGCAGC CAGTCTACAC GTCAGGGATA TACAACGTGC AGGGAGACAG TAACACTAGT 
ATCTGCATCA CCATCGAACC AGGCCTCCTC CTGAAGGGAG ACATCCTGCT GAAGTGTTAC 
CACAAACGAT ACAGAGGCCC GACCAGAGAC GTAGTGTTCC GGGTGCAGTT CCACACCTGT 
GCCATCCACG ACCTTGGCGT GGTGTTCGGG AAGAGCGAAC TGGACGAGAC CTTCAAAGAC 
GACAGGTTTC CTGAATACGG GAAAGTGGAG TTTGTTTTCT CCTATGCACC AGAAAAAATT 
AGAGGCTTAG ACCACCTGGA GAACGGCCCC AGTGTTTCCG TGGACTACAA CACCCAGGAA 
AACCTGATTC GCTGTGATTC ATATGACAAC TTTAGCCAGC GCTCTGAAGA TGCCGTGGAT 
GGAGAGCGTG AGGTCATTCA CACTCAAGGT CCACTTGATG GAAGCCTTTA TGCCCGGGTC 
TGTAAGAAAG ACTCCCTGGA GGGAGTTGTC GCCATCAACG GTAATCCAGT CCATGAAAAT 
TCCTTAGCCA GCACAGAGAA CCAGTTACAA CACACAAGCT CCTTGCTTCA AACCGCCGAA 
CAGACCCTTC CTGTGATTGG CCACGCTTCT CTTCCTGCTG TTGACCACAC ACTCTCTGTG 
AGCAGTGACT CGGGCAACTC CACTGCATCC ATTAAAACTG ATCGTACCGA CGAGCACAGC 
CAGCCAGTGC ACAGCACTGT CAACCACAAC AATCCGGCAG TTCCCCACCC GACACTTAGT 
CCCCAGGAGA AAAAAGAGCT TGATCAGCTC CTTAGTGGCC TCGAAGCACC GACTCTTCAA 
CGACAAGCCT ACCTGGCTAC ATCCACCAGT CCGGGAGGCG GTGTCCGACA CCTGGTCCCA 
GCACAAGTGC ACGTTAACGG AGGCCACCCA AGGCTAGTTC CTGTCCCCTC AACGGAAGAG 
CGTGAGACGG ACATTCTTGA CGACGAGCTG CCAAATAGCC AAGAGGGAAA CAGTGTGGAC 
AGCCTGGGTA CACTGTCATC CTTAGAGGGT CAAGCAACCC CAGGGGATCT CTATTACCAA 
CAAGAGAAGT CCGTCAGCAG ACAAAACGAC GGGCCATATC TTGAGAGAGG CGTTTGTCGG 
GAAAAAAAAA ACGAGATGCC CATGCATGGA GTTCATACTC CGATGATGCA GGAAAGACCT 
GTGGACTCTG TATCCCCACA AGGGGGATAT AACAAATACC AGAACGGTGG TGGGATGTAC 
CACTCTGAAT CCTATGGGAT TCCACCTGCC AGCAGCCCGG AGGGAAACCC TAAACTGATG 
CCCAGGGCCC CAGAGAGAAG CACAAGTAGC CGGGAGGCTG TTCAAAGGGG CCTCAATGCC 
TGGCACCAGT ATAGCCTCCC AGATGATCCA TTTGGACCTC CCCTGCAGTC AACCCATAGC 
ATGCCACAGT TCCCCACAGC AGCCTCACAG CGGGACATAG AGCAGTCCAT CGAGGCACTG 
AATATGCTCA TGCTTGACCT GGACCCCATT AACTCTCACA TGTCCAAGTC CCATAGTGCA 
CCCTCCGGGG AGAACATCCT GAATTCATCC CAGGCACCCT TCTCCCAGAC CCTCTCCAGA 
CCCTCATACC AAGGAGACTC CGCCATCCAC AGATACAATA ACTCTGAATC TGTCAACAAT 
CCCTCCCATC AGCCCATATG GTCAGCTGGA AGAGTGTCAG CAGTGCCTAA CCAGAGCCCT 
ATTATGGATT CTCAAGCACA TTTGCCCCAG AGATCCAGCT CGAGCTGTCA ACACCAGAAA 
ACCACCCCAA CACACACCCC AGAGCCCAAT CTCCATGCAC GACAAGGAGC CATCCATTAC 
CCCATTGATC CCTCCTTTAA TTTCAACCAG CATCTCCCAG TTAGGCCTGC AAACACTTTC 
CCAGGTGTTT CCCCCTCCCC AGACCTGCAG GGTTCGTCTC CATACCAGGG CTTCAGTGCC 
TCTTCCTCAC CCCTTCCTGC GCTCACTCCA CAGCCAAAGA ACACATCGTC TTCATCCCTG 
CCTCAAGAAC AAGATGTTGA GGAAGAGGCG CTGAACTTGG AAGGTCTGGT GGCTCATCGC 
ATTGCCGGCG TTCGCTCCAG AGGAATGACC CCAGAAGTGA CACAGGAGAG CGGTCGACGT 
AGGACCACCA GTGAGGGACA GTACCAGAGC AGCCACGACA GCACACCAAT GGTTGATTCA 
CCGGACTTTG CCCACAGTTT GACCCTCAAC CCTGGAGGCA GACCCAGAGA GGGCCAGATT 
CACAGCTACA GGGAGGCGTT GGAGGACAGC GAGGTGGACC TTGTCATGCA CAGTCCCACC 
TTCAGAGGCG GTGGTGAGAA TTCCCCTCAG ACCCCCAAGT TCCCTGTGTC ACCACAGACT 
CCTTACTTTA ACATGTCTCG TGTCCCTCCA GGTCTGGCCA AGACTCCTCT GTCAGCACTG 
GAACTGAAGC CCCATGATCA CGACTCTGAT TCAAATGAGG GAGAGCAAGA TAACCACACT 
TCCAGGGACT CAAGAGTTCA ACCCTTCAAC ACCTCTTTCT CTTCCACCTA CCCTAGTCAC 
AGCAGTGATG GAAGAAGAAC CGGTTCTTCA GACGTTCACA GCCACACCCC CTCCTACGCA 
AACAGACCCA CCTCCGCTGA GGGCTCCCAG GTCAACGTCA TGGGAGTCCA CACAGTGCCC 
GGCAGTCCCA ACACCCTCCA TCGCACCGTA GCCACCAACA CTCCACCAAG CCCGGCCCTT 
CAGAGACGTC TGGGTCAAGC CAGTCCCTCA CTAGCCAGAC ACCAACCCCC AGCTGGTGCC 
AACAGGGACA GTGTCCCCTC CAGTCCTCTG ATTGGCCGCA ACCCTAAAAC AGCAGCTGCT 
GGCGTGCCCC CCAGCCCTCT AATGGGCCGC CGAACGGCGA TGAGCGGCCA CAGCACGCCT 
GATGACATCG GCCCACCACC TTCTACGCCT GCCTTCCCTG TGTCCCCACA GCTACCAGAA 
AAGAGGCACA TGTCCACTGG AGACGTGGAG AGGACAGATA ACAAGAACCT TATGGCCGGT 
GTTGGCAGCA GTGCGCCAAA CCTGTCTGGC ACGCACACGT TGACAGACGT CCAAAAGTCC 
ATCTATGATG GGTATCCGGA CATTAAGATG AATGTGAAGT TTGTCCAAGA CACATCCAAG 
TTCTGGTACA AGCCAGACAT CTCCAGAGAG CAAGCCATCA ATCTGTTGAA GGACAGAGAG 
CCCGGGGCCT TCATCATAAG GGACAGCCAT TCCTTCCGTG GAGCTTACGG CCTTGCAATG 
AAGGTGGCCT GCCCGCCGCC AACTGTCCAG CAGAACAAAA AAGTTGGAGA CTTGACCAAC 
GAGCTTGTGA GACACTTCCT GATTGAGACA AGCTCCAGAG GTGTTCGACT CAAAGGCTGT 
CCCAATGAAC CCTACTTTGG TTGCCTCTCA GCGCTGGTTT ATCAACACTC AATGACCCCG 
CTAGCTCTGC CCTGTAAGCT GATGATCCCC ACCAAAGACC CGAATGAGGA AGCGCTGGAG 
TTGGCCACAC CCACTGACCC AGTTGTCGAG CTTCTGAAGC AAGGAGCAGT TCAGAAGGTT 
CCAGAGGAGG CTTACGCGTG TAACGTCCTC TACATCAACT CGGTGGACAT GGAGTCTCTC 
ACGGGGCCCC AGGCCATCGC CAAGGCGATC AGCCTGACTT TGGCGACAAA CCCCCTGCCG 
GCCGCCACCA CCGTCCACTT CAAAGTGTCT ATGCAGGGCA TCACGCTCAC CGACAGCCAG 
AGGAAACTTT TTTTCAGACG CCACTATCCC ATCAACACCA TAACGTTCTG TGACATTGAC 
CCGCAGAACA GGAAATGGGG AAAAGAAGGA GGCGGTTCAG TGAAGCTGTT TGGTTTTGTG 
GCACGGAAAC AAGGAAGCAC AACCGACAAC GTGAGCCACC TGTTCGCAGA GCTGGACCCA 
GATCAGCCGG CTTCCGCCAT CACCAGTTTT GTCTCCAAGA CGATGAAGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604681.1
CDS	69..5042
Translation	MPSVSLSLPSTLAGRTRTWVCLSCMFWPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAGVRSRGMTPEVTQESGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREALEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_011606379.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes tensin 1 (tns1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606379.1

ORF Insert Sequence:

ATGCCCTCGG TCTCTCTGTC GTTGCCCTCC ACCCTGGCTG GGAGGACCAG GACCTGGGTT 
TGCCTCTCCT GCATGTTCTG GCCGGATGAG CTGGAGGGCG TCCACACTCA CACCTTCAAG 
CTGAAGCCAT TCAAGAAGGC CAAAAGCTGT GACATCTGTA AGCAGGCCAT CACCAAGGAG 
GGCCTCGTCT GCAAAGCGTG TCGGCTCTCC TGCCACAAAA AATGTGAAGT GAAGGTCACC 
ACATCATGTC AGACGTCCAC CAACCATGAG CTGGGATCTG CGAGGTCCTG CAAGAGTGTG 
GAGATAAGGC GGAAGCAGTC TCGAAGCCAG AGTGTGGTTC TGACCATGGA GGAGAACTAT 
GAGGTAGACC TGGTCTACAT CACGGAGAGG ATCATCTCCC TCTGCTTCCC CATTGGCATG 
GAGGACCAGG GCTACATCGC CAACCTCAAG GAGGTGGCCA CCATGCTGCG ATCCAAACAT 
GGAGATCATT ATTTGGTGCT GAACCTGAGT GAATGGAGGA CTGAGTTATC GAAGCTAAAC 
CACAAGGTCT TGGAATTTGG CTGGCCGGAC TTTCACGCAC CAGCCCTGGA CAAGATCTGT 
AGCATGTGCA AAGCGATTGA CACGTGGCTC AATGGAGACT CGCACAATGT GGTGGTGCTT 
CACAACAAGG GGAACCGAGG TCGAACTGGG GTCGTGGTGG CTGCTTACAT GCACTATAGC 
AACATATCAG CAAGTGCTGA CCAGGCCTTG GACAGGTTCG CCATGAGACG GTTCTACGAG 
GATAAAGCAC TTCCTGTGGG TCAGCCGTCA CAAAAAAGGT ATGTGCGATA CTTCAATGGC 
CTTCTGTCGG GGCACATCAA AATCAACAAC AAACCTCTCT TCCTGCACCA CGTCATCATG 
CACGGTATCC CCAACTTTGA GTCTAAAGGA GGCTGCCGCC CTTTCCTGAA GATCTACCAA 
GCGATGCAGC CAGTCTACAC GTCAGGGATA TACAACGTGC AGGGAGACAG TAACACTAGT 
ATCTGCATCA CCATCGAACC AGGCCTCCTC CTGAAGGGAG ACATCCTGCT GAAGTGTTAC 
CACAAACGAT ACAGAGGCCC GACCAGAGAC GTAGTGTTCC GGGTGCAGTT CCACACCTGT 
GCCATCCACG ACCTTGGCGT GGTGTTCGGG AAGAGCGAAC TGGACGAGAC CTTCAAAGAC 
GACAGGTTTC CTGAATACGG GAAAGTGGAG TTTGTTTTCT CCTATGCACC AGAAAAAATT 
AGAGGCTTAG ACCACCTGGA GAACGGCCCC AGTGTTTCCG TGGACTACAA CACCCAGGAA 
AACCTGATTC GCTGTGATTC ATATGACAAC TTTAGCCAGC GCTCTGAAGA TGCCGTGGAT 
GGAGAGCGTG AGGTCATTCA CACTCAAGGT CCACTTGATG GAAGCCTTTA TGCCCGGGTC 
TGTAAGAAAG ACTCCCTGGA GGGAGTTGTC GCCATCAACG GTAATCCAGT CCATGAAAAT 
TCCTTAGCCA GCACAGAGAA CCAGTTACAA CACACAAGCT CCTTGCTTCA AACCGCCGAA 
CAGACCCTTC CTGTGATTGG CCACGCTTCT CTTCCTGCTG TTGACCACAC ACTCTCTGTG 
AGCAGTGACT CGGGCAACTC CACTGCATCC ATTAAAACTG ATCGTACCGA CGAGCACAGC 
CAGCCAGTGC ACAGCACTGT CAACCACAAC AATCCGGCAG TTCCCCACCC GACACTTAGT 
CCCCAGGAGA AAAAAGAGCT TGATCAGCTC CTTAGTGGCC TCGAAGCACC GACTCTTCAA 
CGACAAGCCT ACCTGGCTAC ATCCACCAGT CCGGGAGGCG GTGTCCGACA CCTGGTCCCA 
GCACAAGTGC ACGTTAACGG AGGCCACCCA AGGCTAGTTC CTGTCCCCTC AACGGAAGAG 
CGTGAGACGG ACATTCTTGA CGACGAGCTG CCAAATAGCC AAGAGGGAAA CAGTGTGGAC 
AGCCTGGGTA CACTGTCATC CTTAGAGGGT CAAGCAACCC CAGGGGATCT CTATTACCAA 
CAAGAGAAGT CCGTCAGCAG ACAAAACGAC GGGCCATATC TTGAGAGAGG CGTTTGTCGG 
GAAAAAAAAA ACGAGATGCC CATGCATGGA GTTCATACTC CGATGATGCA GGAAAGACCT 
GTGGACTCTG TATCCCCACA AGGGGGATAT AACAAATACC AGAACGGTGG TGGGATGTAC 
CACTCTGAAT CCTATGGGAT TCCACCTGCC AGCAGCCCGG AGGGAAACCC TAAACTGATG 
CCCAGGGCCC CAGAGAGAAG CACAAGTAGC CGGGAGGCTG TTCAAAGGGG CCTCAATGCC 
TGGCACCAGT ATAGCCTCCC AGATGATCCA TTTGGACCTC CCCTGCAGTC AACCCATAGC 
ATGCCACAGT TCCCCACAGC AGCCTCACAG CGGGACATAG AGCAGTCCAT CGAGGCACTG 
AATATGCTCA TGCTTGACCT GGACCCCATT AACTCTCACA TGTCCAAGTC CCATAGTGCA 
CCCTCCGGGG AGAACATCCT GAATTCATCC CAGGCACCCT TCTCCCAGAC CCTCTCCAGA 
CCCTCATACC AAGGAGACTC CGCCATCCAC AGATACAATA ACTCTGAATC TGTCAACAAT 
CCCTCCCATC AGCCCATATG GTCAGCTGGA AGAGTGTCAG CAGTGCCTAA CCAGAGCCCT 
ATTATGGATT CTCAAGCACA TTTGCCCCAG AGATCCAGCT CGAGCTGTCA ACACCAGAAA 
ACCACCCCAA CACACACCCC AGAGCCCAAT CTCCATGCAC GACAAGGAGC CATCCATTAC 
CCCATTGATC CCTCCTTTAA TTTCAACCAG CATCTCCCAG TTAGGCCTGC AAACACTTTC 
CCAGGTGTTT CCCCCTCCCC AGACCTGCAG GGTTCGTCTC CATACCAGGG CTTCAGTGCC 
TCTTCCTCAC CCCTTCCTGC GCTCACTCCA CAGCCAAAGA ACACATCGTC TTCATCCCTG 
CCTCAAGAAC AAGATGTTGA GGAAGAGGCG CTGAACTTGG AAGGTCTGGT GGCTCATCGC 
ATTGCCGGCG TTCGCTCCAG AGGAATGACC CCAGAAGTGA CACAGGAGAG CGGTCGACGT 
AGGACCACCA GTGAGGGACA GTACCAGAGC AGCCACGACA GCACACCAAT GGTTGATTCA 
CCGGACTTTG CCCACAGTTT GACCCTCAAC CCTGGAGGCA GACCCAGAGA GGGCCAGATT 
CACAGCTACA GGGAGGCGTT GGAGGACAGC GAGGTGGACC TTGTCATGCA CAGTCCCACC 
TTCAGAGGCG GTGGTGAGAA TTCCCCTCAG ACCCCCAAGT TCCCTGTGTC ACCACAGACT 
CCTTACTTTA ACATGTCTCG TGTCCCTCCA GGTCTGGCCA AGACTCCTCT GTCAGCACTG 
GAACTGAAGC CCCATGATCA CGACTCTGAT TCAAATGAGG GAGAGCAAGA TAACCACACT 
TCCAGGGACT CAAGAGTTCA ACCCTTCAAC ACCTCTTTCT CTTCCACCTA CCCTAGTCAC 
AGCAGTGATG GAAGAAGAAC CGGTTCTTCA GACGTTCACA GCCACACCCC CTCCTACGCA 
AACAGACCCA CCTCCGCTGA GGGCTCCCAG GTCAACGTCA TGGGAGTCCA CACAGTGCCC 
GGCAGTCCCA ACACCCTCCA TCGCACCGTA GCCACCAACA CTCCACCAAG CCCGGCCCTT 
CAGAGACGTC TGGGTCAAGC CAGTCCCTCA CTAGCCAGAC ACCAACCCCC AGCTGGTGCC 
AACAGGGACA GTGTCCCCTC CAGTCCTCTG ATTGGCCGCA ACCCTAAAAC AGCAGCTGCT 
GGCGTGCCCC CCAGCCCTCT AATGGGCCGC CGAACGGCGA TGAGCGGCCA CAGCACGCCT 
GATGACATCG GCCCACCACC TTCTACGCCT GCCTTCCCTG TGTCCCCACA GCTACCAGAA 
AAGAGGCACA TGTCCACTGG AGACGTGGAG AGGACAGATA ACAAGAACCT TATGGCCGGT 
GTTGGCAGCA GTGCGCCAAA CCTGTCTGGC ACGCACACGT TGACAGACGT CCAAAAGTCC 
ATCTATGATG GGTATCCGGA CATTAAGATG AATGTGAAGT TTGTCCAAGA CACATCCAAG 
TTCTGGTACA AGCCAGACAT CTCCAGAGAG CAAGCCATCA ATCTGTTGAA GGACAGAGAG 
CCCGGGGCCT TCATCATAAG GGACAGCCAT TCCTTCCGTG GAGCTTACGG CCTTGCAATG 
AAGGTGGCCT GCCCGCCGCC AACTGTCCAG CAGAACAAAA AAGTTGGAGA CTTGACCAAC 
GAGCTTGTGA GACACTTCCT GATTGAGACA AGCTCCAGAG GTGTTCGACT CAAAGGCTGT 
CCCAATGAAC CCTACTTTGG TTGCCTCTCA GCGCTGGTTT ATCAACACTC AATGACCCCG 
CTAGCTCTGC CCTGTAAGCT GATGATCCCC ACCAAAGACC CGAATGAGGA AGCGCTGGAG 
TTGGCCACAC CCACTGACCC AGTTGTCGAG CTTCTGAAGC AAGGAGCAGT TCAGAAGGTT 
CCAGAGGAGG CTTACGCGTG TAACGTCCTC TACATCAACT CGGTGGACAT GGAGTCTCTC 
ACGGGGCCCC AGGCCATCGC CAAGGCGATC AGCCTGACTT TGGCGACAAA CCCCCTGCCG 
GCCGCCACCA CCGTCCACTT CAAAGTGTCT ATGCAGGGCA TCACGCTCAC CGACAGCCAG 
AGGAAACTTT TTTTCAGACG CCACTATCCC ATCAACACCA TAACGTTCTG TGACATTGAC 
CCGCAGAACA GGAAATGGGG AAAAGAAGGA GGCGGTTCAG TGAAGCTGTT TGGTTTTGTG 
GCACGGAAAC AAGGAAGCAC AACCGACAAC GTGAGCCACC TGTTCGCAGA GCTGGACCCA 
GATCAGCCGG CTTCCGCCAT CACCAGTTTT GTCTCCAAGA CGATGAAGCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_011606380.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604682.1 Takifugu rubripes tensin 1 (tns1), transcript variant X4, mRNA.

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CloneID	OTo20984
Clone ID Related Accession (Same CDS sequence)	XM_011606380.1
Accession Version	XM_011606380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	tensin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070998.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG TCTCTCTGTC GTTGCCCTCC ACCCTGGCTG GGAGGACCAG GACCTGGGTT 
TGCCTCTCCT GCATGTTCTG GCCGGATGAG CTGGAGGGCG TCCACACTCA CACCTTCAAG 
CTGAAGCCAT TCAAGAAGGC CAAAAGCTGT GACATCTGTA AGCAGGCCAT CACCAAGGAG 
GGCCTCGTCT GCAAAGCGTG TCGGCTCTCC TGCCACAAAA AATGTGAAGT GAAGGTCACC 
ACATCATGTC AGACGTCCAC CAACCATGAG CTGCCCCCCA CTCCGCAGCT GCCATTAAAG 
CATGTAGACA CGCCGGGATC TGCGAGGTCC TGCAAGAGTG TGGAGATAAG GCGGAAGCAG 
TCTCGAAGCC AGAGTGTGGT TCTGACCATG GAGGAGAACT ATGAGGTAGA CCTGGTCTAC 
ATCACGGAGA GGATCATCTC CCTCTGCTTC CCCATTGGCA TGGAGGACCA GGGCTACATC 
GCCAACCTCA AGGAGGTGGC CACCATGCTG CGATCCAAAC ATGGAGATCA TTATTTGGTG 
CTGAACCTGA GTGAATGGAG GACTGAGTTA TCGAAGCTAA ACCACAAGGT CTTGGAATTT 
GGCTGGCCGG ACTTTCACGC ACCAGCCCTG GACAAGATCT GTAGCATGTG CAAAGCGATT 
GACACGTGGC TCAATGGAGA CTCGCACAAT GTGGTGGTGC TTCACAACAA GGGGAACCGA 
GGTCGAACTG GGGTCGTGGT GGCTGCTTAC ATGCACTATA GCAACATATC AGCAAGTGCT 
GACCAGGCCT TGGACAGGTT CGCCATGAGA CGGTTCTACG AGGATAAAGC ACTTCCTGTG 
GGTCAGCCGT CACAAAAAAG GTATGTGCGA TACTTCAATG GCCTTCTGTC GGGGCACATC 
AAAATCAACA ACAAACCTCT CTTCCTGCAC CACGTCATCA TGCACGGTAT CCCCAACTTT 
GAGTCTAAAG GAGGCTGCCG CCCTTTCCTG AAGATCTACC AAGCGATGCA GCCAGTCTAC 
ACGTCAGGGA TATACAACGT GCAGGGAGAC AGTAACACTA GTATCTGCAT CACCATCGAA 
CCAGGCCTCC TCCTGAAGGG AGACATCCTG CTGAAGTGTT ACCACAAACG ATACAGAGGC 
CCGACCAGAG ACGTAGTGTT CCGGGTGCAG TTCCACACCT GTGCCATCCA CGACCTTGGC 
GTGGTGTTCG GGAAGAGCGA ACTGGACGAG ACCTTCAAAG ACGACAGGTT TCCTGAATAC 
GGGAAAGTGG AGTTTGTTTT CTCCTATGCA CCAGAAAAAA TTAGAGGCTT AGACCACCTG 
GAGAACGGCC CCAGTGTTTC CGTGGACTAC AACACCCAGG AAAACCTGAT TCGCTGTGAT 
TCATATGACA ACTTTAGCCA GCGCTCTGAA GATGCCGTGG ATGGAGAGCG TGAGGTCATT 
CACACTCAAG GTCCACTTGA TGGAAGCCTT TATGCCCGGG TCTGTAAGAA AGACTCCCTG 
GAGGGAGTTG TCGCCATCAA CGGTAATCCA GTCCATGAAA ATTCCTTAGC CAGCACAGAG 
AACCAGTTAC AACACACAAG CTCCTTGCTT CAAACCGCCG AACAGACCCT TCCTGTGATT 
GGCCACGCTT CTCTTCCTGC TGTTGACCAC ACACTCTCTG TGAGCAGTGA CTCGGGCAAC 
TCCACTGCAT CCATTAAAAC TGATCGTACC GACGAGCACA GCCAGCCAGT GCACAGCACT 
GTCAACCACA ACAATCCGGC AGTTCCCCAC CCGACACTTA GTCCCCAGGA GAAAAAAGAG 
CTTGATCAGC TCCTTAGTGG CCTCGAAGCA CCGACTCTTC AACGACAAGC CTACCTGGCT 
ACATCCACCA GTCCGGGAGG CGGTGTCCGA CACCTGGTCC CAGCACAAGT GCACGTTAAC 
GGAGGCCACC CAAGGCTAGT TCCTGTCCCC TCAACGGAAG AGCGTGAGAC GGACATTCTT 
GACGACGAGC TGCCAAATAG CCAAGAGGGA AACAGTGTGG ACAGCCTGGG TACACTGTCA 
TCCTTAGAGG GTCAAGCAAC CCCAGGGGAT CTCTATTACC AACAAGAGAA GTCCGTCAGC 
AGACAAAACG ACGGGCCATA TCTTGAGAGA GGCGTTTGTC GGGAAAAAAA AAACGAGATG 
CCCATGCATG GAGTTCATAC TCCGATGATG CAGGAAAGAC CTGTGGACTC TGTATCCCCA 
CAAGGGGGAT ATAACAAATA CCAGAACGGT GGTGGGATGT ACCACTCTGA ATCCTATGGG 
ATTCCACCTG CCAGCAGCCC GGAGGGAAAC CCTAAACTGA TGCCCAGGGC CCCAGAGAGA 
AGCACAAGTA GCCGGGAGGC TGTTCAAAGG GGCCTCAATG CCTGGCACCA GTATAGCCTC 
CCAGATGATC CATTTGGACC TCCCCTGCAG TCAACCCATA GCATGCCACA GTTCCCCACA 
GCAGCCTCAC AGCGGGACAT AGAGCAGTCC ATCGAGGCAC TGAATATGCT CATGCTTGAC 
CTGGACCCCA TTAACTCTCA CATGTCCAAG TCCCATAGTG CACCCTCCGG GGAGAACATC 
CTGAATTCAT CCCAGGCACC CTTCTCCCAG ACCCTCTCCA GACCCTCATA CCAAGGAGAC 
TCCGCCATCC ACAGATACAA TAACTCTGAA TCTGTCAACA ATCCCTCCCA TCAGCCCATA 
TGGTCAGCTG GAAGAGTGTC AGCAGTGCCT AACCAGAGCC CTATTATGGA TTCTCAAGCA 
CATTTGCCCC AGAGATCCAG CTCGAGCTGT CAACACCAGA AAACCACCCC AACACACACC 
CCAGAGCCCA ATCTCCATGC ACGACAAGGA GCCATCCATT ACCCCATTGA TCCCTCCTTT 
AATTTCAACC AGCATCTCCC AGTTAGGCCT GCAAACACTT TCCCAGGTGT TTCCCCCTCC 
CCAGACCTGC AGGGTTCGTC TCCATACCAG GGCTTCAGTG CCTCTTCCTC ACCCCTTCCT 
GCGCTCACTC CACAGCCAAA GAACACATCG TCTTCATCCC TGCCTCAAGA ACAAGATGTT 
GAGGAAGAGG CGCTGAACTT GGAAGGTCTG GTGGCTCATC GCATTGCCGG CGTTCGCTCC 
AGAGGAATGA CCCCAGAAGT GACACAGGAG AGCGGTCGAC GTAGGACCAC CAGTGAGGGA 
CAGTACCAGA GCAGCCACGA CAGCACACCA ATGGTTGATT CACCGGACTT TGCCCACAGT 
TTGACCCTCA ACCCTGGAGG CAGACCCAGA GAGGGCCAGA TTCACAGCTA CAGGGAGGCG 
TTGGAGGACA GCGAGGTGGA CCTTGTCATG CACAGTCCCA CCTTCAGAGG CGGTGCTCGT 
GTCCCTCCAG GTCTGGCCAA GACTCCTCTG TCAGCACTGG AACTGAAGCC CCATGATCAC 
GACTCTGATT CAAATGAGGG AGAGCAAGAT AACCACACTT CCAGGGACTC AAGAGTTCAA 
CCCTTCAACA CCTCTTTCTC TTCCACCTAC CCTAGTCACA GCAGTGATGG AAGAAGAACC 
GGTTCTTCAG ACGTTCACAG CCACACCCCC TCCTACGCAA ACAGACCCAC CTCCGCTGAG 
GGCTCCCAGG TCAACGTCAT GGGAGTCCAC ACAGTGCCCG GCAGTCCCAA CACCCTCCAT 
CGCACCGTAG CCACCAACAC TCCACCAAGC CCGGCCCTTC AGAGACGTCT GGGTCAAGCC 
AGTCCCTCAC TAGCCAGACA CCAACCCCCA GCTGGTGCCA ACAGGGACAG TGTCCCCTCC 
AGTCCTCTGA TTGGCCGCAA CCCTAAAACA GCAGCTGCTG GCGTGCCCCC CAGCCCTCTA 
ATGGGCCGCC GAACGGCGAT GAGCGGCCAC AGCACGCCTG ATGACATCGG CCCACCACCT 
TCTACGCCTG CCTTCCCTGT GTCCCCACAG CTACCAGAAA AGAGGCACAT GTCCACTGGA 
GACGTGGAGA GGACAGATAA CAAGAACCTT ATGGCCGGTG TTGGCAGCAG TGCGCCAAAC 
CTGTCTGGCA CGCACACGTT GACAGACGTC CAAAAGTCCA TCTATGATGG GTATCCGGAC 
ATTAAGATGA ATGTGAAGTT TGTCCAAGAC ACATCCAAGT TCTGGTACAA GCCAGACATC 
TCCAGAGAGC AAGCCATCAA TCTGTTGAAG GACAGAGAGC CCGGGGCCTT CATCATAAGG 
GACAGCCATT CCTTCCGTGG AGCTTACGGC CTTGCAATGA AGGTGGCCTG CCCGCCGCCA 
ACTGTCCAGC AGAACAAAAA AGTTGGAGAC TTGACCAACG AGCTTGTGAG ACACTTCCTG 
ATTGAGACAA GCTCCAGAGG TGTTCGACTC AAAGGCTGTC CCAATGAACC CTACTTTGGT 
TGCCTCTCAG CGCTGGTTTA TCAACACTCA ATGACCCCGC TAGCTCTGCC CTGTAAGCTG 
ATGATCCCCA CCAAAGACCC GAATGAGGAA GCGCTGGAGT TGGCCACACC CACTGACCCA 
GTTGTCGAGC TTCTGAAGCA AGGAGCAGTT CAGAAGGTTC CAGAGGAGGC TTACGCGTGT 
AACGTCCTCT ACATCAACTC GGTGGACATG GAGTCTCTCA CGGGGCCCCA GGCCATCGCC 
AAGGCGATCA GCCTGACTTT GGCGACAAAC CCCCTGCCGG CCGCCACCAC CGTCCACTTC 
AAAGTGTCTA TGCAGGGCAT CACGCTCACC GACAGCCAGA GGAAACTTTT TTTCAGACGC 
CACTATCCCA TCAACACCAT AACGTTCTGT GACATTGACC CGCAGAACAG GAAATGGGGA 
AAAGAAGGAG GCGGTTCAGT GAAGCTGTTT GGTTTTGTGG CACGGAAACA AGGAAGCACA 
ACCGACAACG TGAGCCACCT GTTCGCAGAG CTGGACCCAG ATCAGCCGGC TTCCGCCATC 
ACCAGTTTTG TCTCCAAGAC GATGAAGCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604682.1
CDS	69..5021
Translation	MPSVSLSLPSTLAGRTRTWVCLSCMFWPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELPPTPQLPLKHVDTPGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAGVRSRGMTPEVTQESGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREALEDSEVDLVMHSPTFRGGARVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_011606380.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes tensin 1 (tns1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606380.1

ORF Insert Sequence:

ATGCCCTCGG TCTCTCTGTC GTTGCCCTCC ACCCTGGCTG GGAGGACCAG GACCTGGGTT 
TGCCTCTCCT GCATGTTCTG GCCGGATGAG CTGGAGGGCG TCCACACTCA CACCTTCAAG 
CTGAAGCCAT TCAAGAAGGC CAAAAGCTGT GACATCTGTA AGCAGGCCAT CACCAAGGAG 
GGCCTCGTCT GCAAAGCGTG TCGGCTCTCC TGCCACAAAA AATGTGAAGT GAAGGTCACC 
ACATCATGTC AGACGTCCAC CAACCATGAG CTGCCCCCCA CTCCGCAGCT GCCATTAAAG 
CATGTAGACA CGCCGGGATC TGCGAGGTCC TGCAAGAGTG TGGAGATAAG GCGGAAGCAG 
TCTCGAAGCC AGAGTGTGGT TCTGACCATG GAGGAGAACT ATGAGGTAGA CCTGGTCTAC 
ATCACGGAGA GGATCATCTC CCTCTGCTTC CCCATTGGCA TGGAGGACCA GGGCTACATC 
GCCAACCTCA AGGAGGTGGC CACCATGCTG CGATCCAAAC ATGGAGATCA TTATTTGGTG 
CTGAACCTGA GTGAATGGAG GACTGAGTTA TCGAAGCTAA ACCACAAGGT CTTGGAATTT 
GGCTGGCCGG ACTTTCACGC ACCAGCCCTG GACAAGATCT GTAGCATGTG CAAAGCGATT 
GACACGTGGC TCAATGGAGA CTCGCACAAT GTGGTGGTGC TTCACAACAA GGGGAACCGA 
GGTCGAACTG GGGTCGTGGT GGCTGCTTAC ATGCACTATA GCAACATATC AGCAAGTGCT 
GACCAGGCCT TGGACAGGTT CGCCATGAGA CGGTTCTACG AGGATAAAGC ACTTCCTGTG 
GGTCAGCCGT CACAAAAAAG GTATGTGCGA TACTTCAATG GCCTTCTGTC GGGGCACATC 
AAAATCAACA ACAAACCTCT CTTCCTGCAC CACGTCATCA TGCACGGTAT CCCCAACTTT 
GAGTCTAAAG GAGGCTGCCG CCCTTTCCTG AAGATCTACC AAGCGATGCA GCCAGTCTAC 
ACGTCAGGGA TATACAACGT GCAGGGAGAC AGTAACACTA GTATCTGCAT CACCATCGAA 
CCAGGCCTCC TCCTGAAGGG AGACATCCTG CTGAAGTGTT ACCACAAACG ATACAGAGGC 
CCGACCAGAG ACGTAGTGTT CCGGGTGCAG TTCCACACCT GTGCCATCCA CGACCTTGGC 
GTGGTGTTCG GGAAGAGCGA ACTGGACGAG ACCTTCAAAG ACGACAGGTT TCCTGAATAC 
GGGAAAGTGG AGTTTGTTTT CTCCTATGCA CCAGAAAAAA TTAGAGGCTT AGACCACCTG 
GAGAACGGCC CCAGTGTTTC CGTGGACTAC AACACCCAGG AAAACCTGAT TCGCTGTGAT 
TCATATGACA ACTTTAGCCA GCGCTCTGAA GATGCCGTGG ATGGAGAGCG TGAGGTCATT 
CACACTCAAG GTCCACTTGA TGGAAGCCTT TATGCCCGGG TCTGTAAGAA AGACTCCCTG 
GAGGGAGTTG TCGCCATCAA CGGTAATCCA GTCCATGAAA ATTCCTTAGC CAGCACAGAG 
AACCAGTTAC AACACACAAG CTCCTTGCTT CAAACCGCCG AACAGACCCT TCCTGTGATT 
GGCCACGCTT CTCTTCCTGC TGTTGACCAC ACACTCTCTG TGAGCAGTGA CTCGGGCAAC 
TCCACTGCAT CCATTAAAAC TGATCGTACC GACGAGCACA GCCAGCCAGT GCACAGCACT 
GTCAACCACA ACAATCCGGC AGTTCCCCAC CCGACACTTA GTCCCCAGGA GAAAAAAGAG 
CTTGATCAGC TCCTTAGTGG CCTCGAAGCA CCGACTCTTC AACGACAAGC CTACCTGGCT 
ACATCCACCA GTCCGGGAGG CGGTGTCCGA CACCTGGTCC CAGCACAAGT GCACGTTAAC 
GGAGGCCACC CAAGGCTAGT TCCTGTCCCC TCAACGGAAG AGCGTGAGAC GGACATTCTT 
GACGACGAGC TGCCAAATAG CCAAGAGGGA AACAGTGTGG ACAGCCTGGG TACACTGTCA 
TCCTTAGAGG GTCAAGCAAC CCCAGGGGAT CTCTATTACC AACAAGAGAA GTCCGTCAGC 
AGACAAAACG ACGGGCCATA TCTTGAGAGA GGCGTTTGTC GGGAAAAAAA AAACGAGATG 
CCCATGCATG GAGTTCATAC TCCGATGATG CAGGAAAGAC CTGTGGACTC TGTATCCCCA 
CAAGGGGGAT ATAACAAATA CCAGAACGGT GGTGGGATGT ACCACTCTGA ATCCTATGGG 
ATTCCACCTG CCAGCAGCCC GGAGGGAAAC CCTAAACTGA TGCCCAGGGC CCCAGAGAGA 
AGCACAAGTA GCCGGGAGGC TGTTCAAAGG GGCCTCAATG CCTGGCACCA GTATAGCCTC 
CCAGATGATC CATTTGGACC TCCCCTGCAG TCAACCCATA GCATGCCACA GTTCCCCACA 
GCAGCCTCAC AGCGGGACAT AGAGCAGTCC ATCGAGGCAC TGAATATGCT CATGCTTGAC 
CTGGACCCCA TTAACTCTCA CATGTCCAAG TCCCATAGTG CACCCTCCGG GGAGAACATC 
CTGAATTCAT CCCAGGCACC CTTCTCCCAG ACCCTCTCCA GACCCTCATA CCAAGGAGAC 
TCCGCCATCC ACAGATACAA TAACTCTGAA TCTGTCAACA ATCCCTCCCA TCAGCCCATA 
TGGTCAGCTG GAAGAGTGTC AGCAGTGCCT AACCAGAGCC CTATTATGGA TTCTCAAGCA 
CATTTGCCCC AGAGATCCAG CTCGAGCTGT CAACACCAGA AAACCACCCC AACACACACC 
CCAGAGCCCA ATCTCCATGC ACGACAAGGA GCCATCCATT ACCCCATTGA TCCCTCCTTT 
AATTTCAACC AGCATCTCCC AGTTAGGCCT GCAAACACTT TCCCAGGTGT TTCCCCCTCC 
CCAGACCTGC AGGGTTCGTC TCCATACCAG GGCTTCAGTG CCTCTTCCTC ACCCCTTCCT 
GCGCTCACTC CACAGCCAAA GAACACATCG TCTTCATCCC TGCCTCAAGA ACAAGATGTT 
GAGGAAGAGG CGCTGAACTT GGAAGGTCTG GTGGCTCATC GCATTGCCGG CGTTCGCTCC 
AGAGGAATGA CCCCAGAAGT GACACAGGAG AGCGGTCGAC GTAGGACCAC CAGTGAGGGA 
CAGTACCAGA GCAGCCACGA CAGCACACCA ATGGTTGATT CACCGGACTT TGCCCACAGT 
TTGACCCTCA ACCCTGGAGG CAGACCCAGA GAGGGCCAGA TTCACAGCTA CAGGGAGGCG 
TTGGAGGACA GCGAGGTGGA CCTTGTCATG CACAGTCCCA CCTTCAGAGG CGGTGCTCGT 
GTCCCTCCAG GTCTGGCCAA GACTCCTCTG TCAGCACTGG AACTGAAGCC CCATGATCAC 
GACTCTGATT CAAATGAGGG AGAGCAAGAT AACCACACTT CCAGGGACTC AAGAGTTCAA 
CCCTTCAACA CCTCTTTCTC TTCCACCTAC CCTAGTCACA GCAGTGATGG AAGAAGAACC 
GGTTCTTCAG ACGTTCACAG CCACACCCCC TCCTACGCAA ACAGACCCAC CTCCGCTGAG 
GGCTCCCAGG TCAACGTCAT GGGAGTCCAC ACAGTGCCCG GCAGTCCCAA CACCCTCCAT 
CGCACCGTAG CCACCAACAC TCCACCAAGC CCGGCCCTTC AGAGACGTCT GGGTCAAGCC 
AGTCCCTCAC TAGCCAGACA CCAACCCCCA GCTGGTGCCA ACAGGGACAG TGTCCCCTCC 
AGTCCTCTGA TTGGCCGCAA CCCTAAAACA GCAGCTGCTG GCGTGCCCCC CAGCCCTCTA 
ATGGGCCGCC GAACGGCGAT GAGCGGCCAC AGCACGCCTG ATGACATCGG CCCACCACCT 
TCTACGCCTG CCTTCCCTGT GTCCCCACAG CTACCAGAAA AGAGGCACAT GTCCACTGGA 
GACGTGGAGA GGACAGATAA CAAGAACCTT ATGGCCGGTG TTGGCAGCAG TGCGCCAAAC 
CTGTCTGGCA CGCACACGTT GACAGACGTC CAAAAGTCCA TCTATGATGG GTATCCGGAC 
ATTAAGATGA ATGTGAAGTT TGTCCAAGAC ACATCCAAGT TCTGGTACAA GCCAGACATC 
TCCAGAGAGC AAGCCATCAA TCTGTTGAAG GACAGAGAGC CCGGGGCCTT CATCATAAGG 
GACAGCCATT CCTTCCGTGG AGCTTACGGC CTTGCAATGA AGGTGGCCTG CCCGCCGCCA 
ACTGTCCAGC AGAACAAAAA AGTTGGAGAC TTGACCAACG AGCTTGTGAG ACACTTCCTG 
ATTGAGACAA GCTCCAGAGG TGTTCGACTC AAAGGCTGTC CCAATGAACC CTACTTTGGT 
TGCCTCTCAG CGCTGGTTTA TCAACACTCA ATGACCCCGC TAGCTCTGCC CTGTAAGCTG 
ATGATCCCCA CCAAAGACCC GAATGAGGAA GCGCTGGAGT TGGCCACACC CACTGACCCA 
GTTGTCGAGC TTCTGAAGCA AGGAGCAGTT CAGAAGGTTC CAGAGGAGGC TTACGCGTGT 
AACGTCCTCT ACATCAACTC GGTGGACATG GAGTCTCTCA CGGGGCCCCA GGCCATCGCC 
AAGGCGATCA GCCTGACTTT GGCGACAAAC CCCCTGCCGG CCGCCACCAC CGTCCACTTC 
AAAGTGTCTA TGCAGGGCAT CACGCTCACC GACAGCCAGA GGAAACTTTT TTTCAGACGC 
CACTATCCCA TCAACACCAT AACGTTCTGT GACATTGACC CGCAGAACAG GAAATGGGGA 
AAAGAAGGAG GCGGTTCAGT GAAGCTGTTT GGTTTTGTGG CACGGAAACA AGGAAGCACA 
ACCGACAACG TGAGCCACCT GTTCGCAGAG CTGGACCCAG ATCAGCCGGC TTCCGCCATC 
ACCAGTTTTG TCTCCAAGAC GATGAAGCGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_011606382.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604684.1 Takifugu rubripes tensin 1 (tns1), transcript variant X5, mRNA.

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CloneID	OTo20985
Clone ID Related Accession (Same CDS sequence)	XM_011606382.1
Accession Version	XM_011606382.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	tensin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070998.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG GAAACTCAGT AGAGAAAAGC TTTTCTCACC TGCGTTCATC CTTGAGATGG 
AGCACCGGTC ACCTGTTCAG CAAGGGATCT GCGAGGTCCT GCAAGAGTGT GGAGATAAGG 
CGGAAGCAGT CTCGAAGCCA GAGTGTGGTT CTGACCATGG AGGAGAACTA TGAGGTAGAC 
CTGGTCTACA TCACGGAGAG GATCATCTCC CTCTGCTTCC CCATTGGCAT GGAGGACCAG 
GGCTACATCG CCAACCTCAA GGAGGTGGCC ACCATGCTGC GATCCAAACA TGGAGATCAT 
TATTTGGTGC TGAACCTGAG TGAATGGAGG ACTGAGTTAT CGAAGCTAAA CCACAAGGTC 
TTGGAATTTG GCTGGCCGGA CTTTCACGCA CCAGCCCTGG ACAAGATCTG TAGCATGTGC 
AAAGCGATTG ACACGTGGCT CAATGGAGAC TCGCACAATG TGGTGGTGCT TCACAACAAG 
GGGAACCGAG GTCGAACTGG GGTCGTGGTG GCTGCTTACA TGCACTATAG CAACATATCA 
GCAAGTGCTG ACCAGGCCTT GGACAGGTTC GCCATGAGAC GGTTCTACGA GGATAAAGCA 
CTTCCTGTGG GTCAGCCGTC ACAAAAAAGG TATGTGCGAT ACTTCAATGG CCTTCTGTCG 
GGGCACATCA AAATCAACAA CAAACCTCTC TTCCTGCACC ACGTCATCAT GCACGGTATC 
CCCAACTTTG AGTCTAAAGG AGGCTGCCGC CCTTTCCTGA AGATCTACCA AGCGATGCAG 
CCAGTCTACA CGTCAGGGAT ATACAACGTG CAGGGAGACA GTAACACTAG TATCTGCATC 
ACCATCGAAC CAGGCCTCCT CCTGAAGGGA GACATCCTGC TGAAGTGTTA CCACAAACGA 
TACAGAGGCC CGACCAGAGA CGTAGTGTTC CGGGTGCAGT TCCACACCTG TGCCATCCAC 
GACCTTGGCG TGGTGTTCGG GAAGAGCGAA CTGGACGAGA CCTTCAAAGA CGACAGGTTT 
CCTGAATACG GGAAAGTGGA GTTTGTTTTC TCCTATGCAC CAGAAAAAAT TAGAGGCTTA 
GACCACCTGG AGAACGGCCC CAGTGTTTCC GTGGACTACA ACACCCAGGA AAACCTGATT 
CGCTGTGATT CATATGACAA CTTTAGCCAG CGCTCTGAAG ATGCCGTGGA TGGAGAGCGT 
GAGGTCATTC ACACTCAAGG TCCACTTGAT GGAAGCCTTT ATGCCCGGGT CTGTAAGAAA 
GACTCCCTGG AGGGAGTTGT CGCCATCAAC GGTAATCCAG TCCATGAAAA TTCCTTAGCC 
AGCACAGAGA ACCAGTTACA ACACACAAGC TCCTTGCTTC AAACCGCCGA ACAGACCCTT 
CCTGTGATTG GCCACGCTTC TCTTCCTGCT GTTGACCACA CACTCTCTGT GAGCAGTGAC 
TCGGGCAACT CCACTGCATC CATTAAAACT GATCGTACCG ACGAGCACAG CCAGCCAGTG 
CACAGCACTG TCAACCACAA CAATCCGGCA GTTCCCCACC CGACACTTAG TCCCCAGGAG 
AAAAAAGAGC TTGATCAGCT CCTTAGTGGC CTCGAAGCAC CGACTCTTCA ACGACAAGCC 
TACCTGGCTA CATCCACCAG TCCGGGAGGC GGTGTCCGAC ACCTGGTCCC AGCACAAGTG 
CACGTTAACG GAGGCCACCC AAGGCTAGTT CCTGTCCCCT CAACGGAAGA GCGTGAGACG 
GACATTCTTG ACGACGAGCT GCCAAATAGC CAAGAGGGAA ACAGTGTGGA CAGCCTGGGT 
ACACTGTCAT CCTTAGAGGG TCAAGCAACC CCAGGGGATC TCTATTACCA ACAAGAGAAG 
TCCGTCAGCA GACAAAACGA CGGGCCATAT CTTGAGAGAG GCGTTTGTCG GGAAAAAAAA 
AACGAGATGC CCATGCATGG AGTTCATACT CCGATGATGC AGGAAAGACC TGTGGACTCT 
GTATCCCCAC AAGGGGGATA TAACAAATAC CAGAACGGTG GTGGGATGTA CCACTCTGAA 
TCCTATGGGA TTCCACCTGC CAGCAGCCCG GAGGGAAACC CTAAACTGAT GCCCAGGGCC 
CCAGAGAGAA GCACAAGTAG CCGGGAGGCT GTTCAAAGGG GCCTCAATGC CTGGCACCAG 
TATAGCCTCC CAGATGATCC ATTTGGACCT CCCCTGCAGT CAACCCATAG CATGCCACAG 
TTCCCCACAG CAGCCTCACA GCGGGACATA GAGCAGTCCA TCGAGGCACT GAATATGCTC 
ATGCTTGACC TGGACCCCAT TAACTCTCAC ATGTCCAAGT CCCATAGTGC ACCCTCCGGG 
GAGAACATCC TGAATTCATC CCAGGCACCC TTCTCCCAGA CCCTCTCCAG ACCCTCATAC 
CAAGGAGACT CCGCCATCCA CAGATACAAT AACTCTGAAT CTGTCAACAA TCCCTCCCAT 
CAGCCCATAT GGTCAGCTGG AAGAGTGTCA GCAGTGCCTA ACCAGAGCCC TATTATGGAT 
TCTCAAGCAC ATTTGCCCCA GAGATCCAGC TCGAGCTGTC AACACCAGAA AACCACCCCA 
ACACACACCC CAGAGCCCAA TCTCCATGCA CGACAAGGAG CCATCCATTA CCCCATTGAT 
CCCTCCTTTA ATTTCAACCA GCATCTCCCA GTTAGGCCTG CAAACACTTT CCCAGGTGTT 
TCCCCCTCCC CAGACCTGCA GGGTTCGTCT CCATACCAGG GCTTCAGTGC CTCTTCCTCA 
CCCCTTCCTG CGCTCACTCC ACAGCCAAAG AACACATCGT CTTCATCCCT GCCTCAAGAA 
CAAGATGTTG AGGAAGAGGC GCTGAACTTG GAAGGTCTGG TGGCTCATCG CATTGCCGGC 
GTTCGCTCCA GAGGAATGAC CCCAGAAGTG ACACAGGAGA GCGGTCGACG TAGGACCACC 
AGTGAGGGAC AGTACCAGAG CAGCCACGAC AGCACACCAA TGGTTGATTC ACCGGACTTT 
GCCCACAGTT TGACCCTCAA CCCTGGAGGC AGACCCAGAG AGGGCCAGAT TCACAGCTAC 
AGGGAGGCGT TGGAGGACAG CGAGGTGGAC CTTGTCATGC ACAGTCCCAC CTTCAGAGGC 
GGTGGTGAGA ATTCCCCTCA GACCCCCAAG TTCCCTGTGT CACCACAGAC TCCTTACTTT 
AACATGTCTC GTGTCCCTCC AGGTCTGGCC AAGACTCCTC TGTCAGCACT GGAACTGAAG 
CCCCATGATC ACGACTCTGA TTCAAATGAG GGAGAGCAAG ATAACCACAC TTCCAGGGAC 
TCAAGAGTTC AACCCTTCAA CACCTCTTTC TCTTCCACCT ACCCTAGTCA CAGCAGTGAT 
GGAAGAAGAA CCGGTTCTTC AGACGTTCAC AGCCACACCC CCTCCTACGC AAACAGACCC 
ACCTCCGCTG AGGGCTCCCA GGTCAACGTC ATGGGAGTCC ACACAGTGCC CGGCAGTCCC 
AACACCCTCC ATCGCACCGT AGCCACCAAC ACTCCACCAA GCCCGGCCCT TCAGAGACGT 
CTGGGTCAAG CCAGTCCCTC ACTAGCCAGA CACCAACCCC CAGCTGGTGC CAACAGGGAC 
AGTGTCCCCT CCAGTCCTCT GATTGGCCGC AACCCTAAAA CAGCAGCTGC TGGCGTGCCC 
CCCAGCCCTC TAATGGGCCG CCGAACGGCG ATGAGCGGCC ACAGCACGCC TGATGACATC 
GGCCCACCAC CTTCTACGCC TGCCTTCCCT GTGTCCCCAC AGCTACCAGA AAAGAGGCAC 
ATGTCCACTG GAGACGTGGA GAGGACAGAT AACAAGAACC TTATGGCCGG TGTTGGCAGC 
AGTGCGCCAA ACCTGTCTGG CACGCACACG TTGACAGACG TCCAAAAGTC CATCTATGAT 
GGGTATCCGG ACATTAAGAT GAATGTGAAG TTTGTCCAAG ACACATCCAA GTTCTGGTAC 
AAGCCAGACA TCTCCAGAGA GCAAGCCATC AATCTGTTGA AGGACAGAGA GCCCGGGGCC 
TTCATCATAA GGGACAGCCA TTCCTTCCGT GGAGCTTACG GCCTTGCAAT GAAGGTGGCC 
TGCCCGCCGC CAACTGTCCA GCAGAACAAA AAAGTTGGAG ACTTGACCAA CGAGCTTGTG 
AGACACTTCC TGATTGAGAC AAGCTCCAGA GGTGTTCGAC TCAAAGGCTG TCCCAATGAA 
CCCTACTTTG GTTGCCTCTC AGCGCTGGTT TATCAACACT CAATGACCCC GCTAGCTCTG 
CCCTGTAAGC TGATGATCCC CACCAAAGAC CCGAATGAGG AAGCGCTGGA GTTGGCCACA 
CCCACTGACC CAGTTGTCGA GCTTCTGAAG CAAGGAGCAG TTCAGAAGGT TCCAGAGGAG 
GCTTACGCGT GTAACGTCCT CTACATCAAC TCGGTGGACA TGGAGTCTCT CACGGGGCCC 
CAGGCCATCG CCAAGGCGAT CAGCCTGACT TTGGCGACAA ACCCCCTGCC GGCCGCCACC 
ACCGTCCACT TCAAAGTGTC TATGCAGGGC ATCACGCTCA CCGACAGCCA GAGGAAACTT 
TTTTTCAGAC GCCACTATCC CATCAACACC ATAACGTTCT GTGACATTGA CCCGCAGAAC 
AGGAAATGGG GAAAAGAAGG AGGCGGTTCA GTGAAGCTGT TTGGTTTTGT GGCACGGAAA 
CAAGGAAGCA CAACCGACAA CGTGAGCCAC CTGTTCGCAG AGCTGGACCC AGATCAGCCG 
GCTTCCGCCA TCACCAGTTT TGTCTCCAAG ACGATGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604684.1
CDS	1688..6472
Translation	MSLGNSVEKSFSHLRSSLRWSTGHLFSKGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAGVRSRGMTPEVTQESGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREALEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_011606382.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes tensin 1 (tns1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606382.1

ORF Insert Sequence:

ATGTCTCTGG GAAACTCAGT AGAGAAAAGC TTTTCTCACC TGCGTTCATC CTTGAGATGG 
AGCACCGGTC ACCTGTTCAG CAAGGGATCT GCGAGGTCCT GCAAGAGTGT GGAGATAAGG 
CGGAAGCAGT CTCGAAGCCA GAGTGTGGTT CTGACCATGG AGGAGAACTA TGAGGTAGAC 
CTGGTCTACA TCACGGAGAG GATCATCTCC CTCTGCTTCC CCATTGGCAT GGAGGACCAG 
GGCTACATCG CCAACCTCAA GGAGGTGGCC ACCATGCTGC GATCCAAACA TGGAGATCAT 
TATTTGGTGC TGAACCTGAG TGAATGGAGG ACTGAGTTAT CGAAGCTAAA CCACAAGGTC 
TTGGAATTTG GCTGGCCGGA CTTTCACGCA CCAGCCCTGG ACAAGATCTG TAGCATGTGC 
AAAGCGATTG ACACGTGGCT CAATGGAGAC TCGCACAATG TGGTGGTGCT TCACAACAAG 
GGGAACCGAG GTCGAACTGG GGTCGTGGTG GCTGCTTACA TGCACTATAG CAACATATCA 
GCAAGTGCTG ACCAGGCCTT GGACAGGTTC GCCATGAGAC GGTTCTACGA GGATAAAGCA 
CTTCCTGTGG GTCAGCCGTC ACAAAAAAGG TATGTGCGAT ACTTCAATGG CCTTCTGTCG 
GGGCACATCA AAATCAACAA CAAACCTCTC TTCCTGCACC ACGTCATCAT GCACGGTATC 
CCCAACTTTG AGTCTAAAGG AGGCTGCCGC CCTTTCCTGA AGATCTACCA AGCGATGCAG 
CCAGTCTACA CGTCAGGGAT ATACAACGTG CAGGGAGACA GTAACACTAG TATCTGCATC 
ACCATCGAAC CAGGCCTCCT CCTGAAGGGA GACATCCTGC TGAAGTGTTA CCACAAACGA 
TACAGAGGCC CGACCAGAGA CGTAGTGTTC CGGGTGCAGT TCCACACCTG TGCCATCCAC 
GACCTTGGCG TGGTGTTCGG GAAGAGCGAA CTGGACGAGA CCTTCAAAGA CGACAGGTTT 
CCTGAATACG GGAAAGTGGA GTTTGTTTTC TCCTATGCAC CAGAAAAAAT TAGAGGCTTA 
GACCACCTGG AGAACGGCCC CAGTGTTTCC GTGGACTACA ACACCCAGGA AAACCTGATT 
CGCTGTGATT CATATGACAA CTTTAGCCAG CGCTCTGAAG ATGCCGTGGA TGGAGAGCGT 
GAGGTCATTC ACACTCAAGG TCCACTTGAT GGAAGCCTTT ATGCCCGGGT CTGTAAGAAA 
GACTCCCTGG AGGGAGTTGT CGCCATCAAC GGTAATCCAG TCCATGAAAA TTCCTTAGCC 
AGCACAGAGA ACCAGTTACA ACACACAAGC TCCTTGCTTC AAACCGCCGA ACAGACCCTT 
CCTGTGATTG GCCACGCTTC TCTTCCTGCT GTTGACCACA CACTCTCTGT GAGCAGTGAC 
TCGGGCAACT CCACTGCATC CATTAAAACT GATCGTACCG ACGAGCACAG CCAGCCAGTG 
CACAGCACTG TCAACCACAA CAATCCGGCA GTTCCCCACC CGACACTTAG TCCCCAGGAG 
AAAAAAGAGC TTGATCAGCT CCTTAGTGGC CTCGAAGCAC CGACTCTTCA ACGACAAGCC 
TACCTGGCTA CATCCACCAG TCCGGGAGGC GGTGTCCGAC ACCTGGTCCC AGCACAAGTG 
CACGTTAACG GAGGCCACCC AAGGCTAGTT CCTGTCCCCT CAACGGAAGA GCGTGAGACG 
GACATTCTTG ACGACGAGCT GCCAAATAGC CAAGAGGGAA ACAGTGTGGA CAGCCTGGGT 
ACACTGTCAT CCTTAGAGGG TCAAGCAACC CCAGGGGATC TCTATTACCA ACAAGAGAAG 
TCCGTCAGCA GACAAAACGA CGGGCCATAT CTTGAGAGAG GCGTTTGTCG GGAAAAAAAA 
AACGAGATGC CCATGCATGG AGTTCATACT CCGATGATGC AGGAAAGACC TGTGGACTCT 
GTATCCCCAC AAGGGGGATA TAACAAATAC CAGAACGGTG GTGGGATGTA CCACTCTGAA 
TCCTATGGGA TTCCACCTGC CAGCAGCCCG GAGGGAAACC CTAAACTGAT GCCCAGGGCC 
CCAGAGAGAA GCACAAGTAG CCGGGAGGCT GTTCAAAGGG GCCTCAATGC CTGGCACCAG 
TATAGCCTCC CAGATGATCC ATTTGGACCT CCCCTGCAGT CAACCCATAG CATGCCACAG 
TTCCCCACAG CAGCCTCACA GCGGGACATA GAGCAGTCCA TCGAGGCACT GAATATGCTC 
ATGCTTGACC TGGACCCCAT TAACTCTCAC ATGTCCAAGT CCCATAGTGC ACCCTCCGGG 
GAGAACATCC TGAATTCATC CCAGGCACCC TTCTCCCAGA CCCTCTCCAG ACCCTCATAC 
CAAGGAGACT CCGCCATCCA CAGATACAAT AACTCTGAAT CTGTCAACAA TCCCTCCCAT 
CAGCCCATAT GGTCAGCTGG AAGAGTGTCA GCAGTGCCTA ACCAGAGCCC TATTATGGAT 
TCTCAAGCAC ATTTGCCCCA GAGATCCAGC TCGAGCTGTC AACACCAGAA AACCACCCCA 
ACACACACCC CAGAGCCCAA TCTCCATGCA CGACAAGGAG CCATCCATTA CCCCATTGAT 
CCCTCCTTTA ATTTCAACCA GCATCTCCCA GTTAGGCCTG CAAACACTTT CCCAGGTGTT 
TCCCCCTCCC CAGACCTGCA GGGTTCGTCT CCATACCAGG GCTTCAGTGC CTCTTCCTCA 
CCCCTTCCTG CGCTCACTCC ACAGCCAAAG AACACATCGT CTTCATCCCT GCCTCAAGAA 
CAAGATGTTG AGGAAGAGGC GCTGAACTTG GAAGGTCTGG TGGCTCATCG CATTGCCGGC 
GTTCGCTCCA GAGGAATGAC CCCAGAAGTG ACACAGGAGA GCGGTCGACG TAGGACCACC 
AGTGAGGGAC AGTACCAGAG CAGCCACGAC AGCACACCAA TGGTTGATTC ACCGGACTTT 
GCCCACAGTT TGACCCTCAA CCCTGGAGGC AGACCCAGAG AGGGCCAGAT TCACAGCTAC 
AGGGAGGCGT TGGAGGACAG CGAGGTGGAC CTTGTCATGC ACAGTCCCAC CTTCAGAGGC 
GGTGGTGAGA ATTCCCCTCA GACCCCCAAG TTCCCTGTGT CACCACAGAC TCCTTACTTT 
AACATGTCTC GTGTCCCTCC AGGTCTGGCC AAGACTCCTC TGTCAGCACT GGAACTGAAG 
CCCCATGATC ACGACTCTGA TTCAAATGAG GGAGAGCAAG ATAACCACAC TTCCAGGGAC 
TCAAGAGTTC AACCCTTCAA CACCTCTTTC TCTTCCACCT ACCCTAGTCA CAGCAGTGAT 
GGAAGAAGAA CCGGTTCTTC AGACGTTCAC AGCCACACCC CCTCCTACGC AAACAGACCC 
ACCTCCGCTG AGGGCTCCCA GGTCAACGTC ATGGGAGTCC ACACAGTGCC CGGCAGTCCC 
AACACCCTCC ATCGCACCGT AGCCACCAAC ACTCCACCAA GCCCGGCCCT TCAGAGACGT 
CTGGGTCAAG CCAGTCCCTC ACTAGCCAGA CACCAACCCC CAGCTGGTGC CAACAGGGAC 
AGTGTCCCCT CCAGTCCTCT GATTGGCCGC AACCCTAAAA CAGCAGCTGC TGGCGTGCCC 
CCCAGCCCTC TAATGGGCCG CCGAACGGCG ATGAGCGGCC ACAGCACGCC TGATGACATC 
GGCCCACCAC CTTCTACGCC TGCCTTCCCT GTGTCCCCAC AGCTACCAGA AAAGAGGCAC 
ATGTCCACTG GAGACGTGGA GAGGACAGAT AACAAGAACC TTATGGCCGG TGTTGGCAGC 
AGTGCGCCAA ACCTGTCTGG CACGCACACG TTGACAGACG TCCAAAAGTC CATCTATGAT 
GGGTATCCGG ACATTAAGAT GAATGTGAAG TTTGTCCAAG ACACATCCAA GTTCTGGTAC 
AAGCCAGACA TCTCCAGAGA GCAAGCCATC AATCTGTTGA AGGACAGAGA GCCCGGGGCC 
TTCATCATAA GGGACAGCCA TTCCTTCCGT GGAGCTTACG GCCTTGCAAT GAAGGTGGCC 
TGCCCGCCGC CAACTGTCCA GCAGAACAAA AAAGTTGGAG ACTTGACCAA CGAGCTTGTG 
AGACACTTCC TGATTGAGAC AAGCTCCAGA GGTGTTCGAC TCAAAGGCTG TCCCAATGAA 
CCCTACTTTG GTTGCCTCTC AGCGCTGGTT TATCAACACT CAATGACCCC GCTAGCTCTG 
CCCTGTAAGC TGATGATCCC CACCAAAGAC CCGAATGAGG AAGCGCTGGA GTTGGCCACA 
CCCACTGACC CAGTTGTCGA GCTTCTGAAG CAAGGAGCAG TTCAGAAGGT TCCAGAGGAG 
GCTTACGCGT GTAACGTCCT CTACATCAAC TCGGTGGACA TGGAGTCTCT CACGGGGCCC 
CAGGCCATCG CCAAGGCGAT CAGCCTGACT TTGGCGACAA ACCCCCTGCC GGCCGCCACC 
ACCGTCCACT TCAAAGTGTC TATGCAGGGC ATCACGCTCA CCGACAGCCA GAGGAAACTT 
TTTTTCAGAC GCCACTATCC CATCAACACC ATAACGTTCT GTGACATTGA CCCGCAGAAC 
AGGAAATGGG GAAAAGAAGG AGGCGGTTCA GTGAAGCTGT TTGGTTTTGT GGCACGGAAA 
CAAGGAAGCA CAACCGACAA CGTGAGCCAC CTGTTCGCAG AGCTGGACCC AGATCAGCCG 
GCTTCCGCCA TCACCAGTTT TGTCTCCAAG ACGATGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_011606383.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604685.1 Takifugu rubripes tensin 1 (tns1), transcript variant X6, mRNA.

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CloneID	OTo20986
Clone ID Related Accession (Same CDS sequence)	XM_011606383.1
Accession Version	XM_011606383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	tensin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070998.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACTATGAGGT AGACCTGGTC TACATCACGG AGAGGATCAT CTCCCTCTGC 
TTCCCCATTG GCATGGAGGA CCAGGGCTAC ATCGCCAACC TCAAGGAGGT GGCCACCATG 
CTGCGATCCA AACATGGAGA TCATTATTTG GTGCTGAACC TGAGTGAATG GAGGACTGAG 
TTATCGAAGC TAAACCACAA GGTCTTGGAA TTTGGCTGGC CGGACTTTCA CGCACCAGCC 
CTGGACAAGA TCTGTAGCAT GTGCAAAGCG ATTGACACGT GGCTCAATGG AGACTCGCAC 
AATGTGGTGG TGCTTCACAA CAAGGGGAAC CGAGGTCGAA CTGGGGTCGT GGTGGCTGCT 
TACATGCACT ATAGCAACAT ATCAGCAAGT GCTGACCAGG CCTTGGACAG GTTCGCCATG 
AGACGGTTCT ACGAGGATAA AGCACTTCCT GTGGGTCAGC CGTCACAAAA AAGGTATGTG 
CGATACTTCA ATGGCCTTCT GTCGGGGCAC ATCAAAATCA ACAACAAACC TCTCTTCCTG 
CACCACGTCA TCATGCACGG TATCCCCAAC TTTGAGTCTA AAGGAGGCTG CCGCCCTTTC 
CTGAAGATCT ACCAAGCGAT GCAGCCAGTC TACACGTCAG GGATATACAA CGTGCAGGGA 
GACAGTAACA CTAGTATCTG CATCACCATC GAACCAGGCC TCCTCCTGAA GGGAGACATC 
CTGCTGAAGT GTTACCACAA ACGATACAGA GGCCCGACCA GAGACGTAGT GTTCCGGGTG 
CAGTTCCACA CCTGTGCCAT CCACGACCTT GGCGTGGTGT TCGGGAAGAG CGAACTGGAC 
GAGACCTTCA AAGACGACAG GTTTCCTGAA TACGGGAAAG TGGAGTTTGT TTTCTCCTAT 
GCACCAGAAA AAATTAGAGG CTTAGACCAC CTGGAGAACG GCCCCAGTGT TTCCGTGGAC 
TACAACACCC AGGAAAACCT GATTCGCTGT GATTCATATG ACAACTTTAG CCAGCGCTCT 
GAAGATGCCG TGGATGGAGA GCGTGAGGTC ATTCACACTC AAGGTCCACT TGATGGAAGC 
CTTTATGCCC GGGTCTGTAA GAAAGACTCC CTGGAGGGAG TTGTCGCCAT CAACGGTAAT 
CCAGTCCATG AAAATTCCTT AGCCAGCACA GAGAACCAGT TACAACACAC AAGCTCCTTG 
CTTCAAACCG CCGAACAGAC CCTTCCTGTG ATTGGCCACG CTTCTCTTCC TGCTGTTGAC 
CACACACTCT CTGTGAGCAG TGACTCGGGC AACTCCACTG CATCCATTAA AACTGATCGT 
ACCGACGAGC ACAGCCAGCC AGTGCACAGC ACTGTCAACC ACAACAATCC GGCAGTTCCC 
CACCCGACAC TTAGTCCCCA GGAGAAAAAA GAGCTTGATC AGCTCCTTAG TGGCCTCGAA 
GCACCGACTC TTCAACGACA AGCCTACCTG GCTACATCCA CCAGTCCGGG AGGCGGTGTC 
CGACACCTGG TCCCAGCACA AGTGCACGTT AACGGAGGCC ACCCAAGGCT AGTTCCTGTC 
CCCTCAACGG AAGAGCGTGA GACGGACATT CTTGACGACG AGCTGCCAAA TAGCCAAGAG 
GGAAACAGTG TGGACAGCCT GGGTACACTG TCATCCTTAG AGGGTCAAGC AACCCCAGGG 
GATCTCTATT ACCAACAAGA GAAGTCCGTC AGCAGACAAA ACGACGGGCC ATATCTTGAG 
AGAGGCGTTT GTCGGGAAAA AAAAAACGAG ATGCCCATGC ATGGAGTTCA TACTCCGATG 
ATGCAGGAAA GACCTGTGGA CTCTGTATCC CCACAAGGGG GATATAACAA ATACCAGAAC 
GGTGGTGGGA TGTACCACTC TGAATCCTAT GGGATTCCAC CTGCCAGCAG CCCGGAGGGA 
AACCCTAAAC TGATGCCCAG GGCCCCAGAG AGAAGCACAA GTAGCCGGGA GGCTGTTCAA 
AGGGGCCTCA ATGCCTGGCA CCAGTATAGC CTCCCAGATG ATCCATTTGG ACCTCCCCTG 
CAGTCAACCC ATAGCATGCC ACAGTTCCCC ACAGCAGCCT CACAGCGGGA CATAGAGCAG 
TCCATCGAGG CACTGAATAT GCTCATGCTT GACCTGGACC CCATTAACTC TCACATGTCC 
AAGTCCCATA GTGCACCCTC CGGGGAGAAC ATCCTGAATT CATCCCAGGC ACCCTTCTCC 
CAGACCCTCT CCAGACCCTC ATACCAAGGA GACTCCGCCA TCCACAGATA CAATAACTCT 
GAATCTGTCA ACAATCCCTC CCATCAGCCC ATATGGTCAG CTGGAAGAGT GTCAGCAGTG 
CCTAACCAGA GCCCTATTAT GGATTCTCAA GCACATTTGC CCCAGAGATC CAGCTCGAGC 
TGTCAACACC AGAAAACCAC CCCAACACAC ACCCCAGAGC CCAATCTCCA TGCACGACAA 
GGAGCCATCC ATTACCCCAT TGATCCCTCC TTTAATTTCA ACCAGCATCT CCCAGTTAGG 
CCTGCAAACA CTTTCCCAGG TGTTTCCCCC TCCCCAGACC TGCAGGGTTC GTCTCCATAC 
CAGGGCTTCA GTGCCTCTTC CTCACCCCTT CCTGCGCTCA CTCCACAGCC AAAGAACACA 
TCGTCTTCAT CCCTGCCTCA AGAACAAGAT GTTGAGGAAG AGGCGCTGAA CTTGGAAGGT 
CTGGTGGCTC ATCGCATTGC CGGCGTTCGC TCCAGAGGAA TGACCCCAGA AGTGACACAG 
GAGAGCGGTC GACGTAGGAC CACCAGTGAG GGACAGTACC AGAGCAGCCA CGACAGCACA 
CCAATGGTTG ATTCACCGGA CTTTGCCCAC AGTTTGACCC TCAACCCTGG AGGCAGACCC 
AGAGAGGGCC AGATTCACAG CTACAGGGAG GCGTTGGAGG ACAGCGAGGT GGACCTTGTC 
ATGCACAGTC CCACCTTCAG AGGCGGTGGT GAGAATTCCC CTCAGACCCC CAAGTTCCCT 
GTGTCACCAC AGACTCCTTA CTTTAACATG TCTCGTGTCC CTCCAGGTCT GGCCAAGACT 
CCTCTGTCAG CACTGGAACT GAAGCCCCAT GATCACGACT CTGATTCAAA TGAGGGAGAG 
CAAGATAACC ACACTTCCAG GGACTCAAGA GTTCAACCCT TCAACACCTC TTTCTCTTCC 
ACCTACCCTA GTCACAGCAG TGATGGAAGA AGAACCGGTT CTTCAGACGT TCACAGCCAC 
ACCCCCTCCT ACGCAAACAG ACCCACCTCC GCTGAGGGCT CCCAGGTCAA CGTCATGGGA 
GTCCACACAG TGCCCGGCAG TCCCAACACC CTCCATCGCA CCGTAGCCAC CAACACTCCA 
CCAAGCCCGG CCCTTCAGAG ACGTCTGGGT CAAGCCAGTC CCTCACTAGC CAGACACCAA 
CCCCCAGCTG GTGCCAACAG GGACAGTGTC CCCTCCAGTC CTCTGATTGG CCGCAACCCT 
AAAACAGCAG CTGCTGGCGT GCCCCCCAGC CCTCTAATGG GCCGCCGAAC GGCGATGAGC 
GGCCACAGCA CGCCTGATGA CATCGGCCCA CCACCTTCTA CGCCTGCCTT CCCTGTGTCC 
CCACAGCTAC CAGAAAAGAG GCACATGTCC ACTGGAGACG TGGAGAGGAC AGATAACAAG 
AACCTTATGG CCGGTGTTGG CAGCAGTGCG CCAAACCTGT CTGGCACGCA CACGTTGACA 
GACGTCCAAA AGTCCATCTA TGATGGGTAT CCGGACATTA AGATGAATGT GAAGTTTGTC 
CAAGACACAT CCAAGTTCTG GTACAAGCCA GACATCTCCA GAGAGCAAGC CATCAATCTG 
TTGAAGGACA GAGAGCCCGG GGCCTTCATC ATAAGGGACA GCCATTCCTT CCGTGGAGCT 
TACGGCCTTG CAATGAAGGT GGCCTGCCCG CCGCCAACTG TCCAGCAGAA CAAAAAAGTT 
GGAGACTTGA CCAACGAGCT TGTGAGACAC TTCCTGATTG AGACAAGCTC CAGAGGTGTT 
CGACTCAAAG GCTGTCCCAA TGAACCCTAC TTTGGTTGCC TCTCAGCGCT GGTTTATCAA 
CACTCAATGA CCCCGCTAGC TCTGCCCTGT AAGCTGATGA TCCCCACCAA AGACCCGAAT 
GAGGAAGCGC TGGAGTTGGC CACACCCACT GACCCAGTTG TCGAGCTTCT GAAGCAAGGA 
GCAGTTCAGA AGGTTCCAGA GGAGGCTTAC GCGTGTAACG TCCTCTACAT CAACTCGGTG 
GACATGGAGT CTCTCACGGG GCCCCAGGCC ATCGCCAAGG CGATCAGCCT GACTTTGGCG 
ACAAACCCCC TGCCGGCCGC CACCACCGTC CACTTCAAAG TGTCTATGCA GGGCATCACG 
CTCACCGACA GCCAGAGGAA ACTTTTTTTC AGACGCCACT ATCCCATCAA CACCATAACG 
TTCTGTGACA TTGACCCGCA GAACAGGAAA TGGGGAAAAG AAGGAGGCGG TTCAGTGAAG 
CTGTTTGGTT TTGTGGCACG GAAACAAGGA AGCACAACCG ACAACGTGAG CCACCTGTTC 
GCAGAGCTGG ACCCAGATCA GCCGGCTTCC GCCATCACCA GTTTTGTCTC CAAGACGATG 
AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604685.1
CDS	431..5059
Translation	MEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAGVRSRGMTPEVTQESGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREALEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_011606383.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes tensin 1 (tns1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606383.1

ORF Insert Sequence:

ATGGAGGAGA ACTATGAGGT AGACCTGGTC TACATCACGG AGAGGATCAT CTCCCTCTGC 
TTCCCCATTG GCATGGAGGA CCAGGGCTAC ATCGCCAACC TCAAGGAGGT GGCCACCATG 
CTGCGATCCA AACATGGAGA TCATTATTTG GTGCTGAACC TGAGTGAATG GAGGACTGAG 
TTATCGAAGC TAAACCACAA GGTCTTGGAA TTTGGCTGGC CGGACTTTCA CGCACCAGCC 
CTGGACAAGA TCTGTAGCAT GTGCAAAGCG ATTGACACGT GGCTCAATGG AGACTCGCAC 
AATGTGGTGG TGCTTCACAA CAAGGGGAAC CGAGGTCGAA CTGGGGTCGT GGTGGCTGCT 
TACATGCACT ATAGCAACAT ATCAGCAAGT GCTGACCAGG CCTTGGACAG GTTCGCCATG 
AGACGGTTCT ACGAGGATAA AGCACTTCCT GTGGGTCAGC CGTCACAAAA AAGGTATGTG 
CGATACTTCA ATGGCCTTCT GTCGGGGCAC ATCAAAATCA ACAACAAACC TCTCTTCCTG 
CACCACGTCA TCATGCACGG TATCCCCAAC TTTGAGTCTA AAGGAGGCTG CCGCCCTTTC 
CTGAAGATCT ACCAAGCGAT GCAGCCAGTC TACACGTCAG GGATATACAA CGTGCAGGGA 
GACAGTAACA CTAGTATCTG CATCACCATC GAACCAGGCC TCCTCCTGAA GGGAGACATC 
CTGCTGAAGT GTTACCACAA ACGATACAGA GGCCCGACCA GAGACGTAGT GTTCCGGGTG 
CAGTTCCACA CCTGTGCCAT CCACGACCTT GGCGTGGTGT TCGGGAAGAG CGAACTGGAC 
GAGACCTTCA AAGACGACAG GTTTCCTGAA TACGGGAAAG TGGAGTTTGT TTTCTCCTAT 
GCACCAGAAA AAATTAGAGG CTTAGACCAC CTGGAGAACG GCCCCAGTGT TTCCGTGGAC 
TACAACACCC AGGAAAACCT GATTCGCTGT GATTCATATG ACAACTTTAG CCAGCGCTCT 
GAAGATGCCG TGGATGGAGA GCGTGAGGTC ATTCACACTC AAGGTCCACT TGATGGAAGC 
CTTTATGCCC GGGTCTGTAA GAAAGACTCC CTGGAGGGAG TTGTCGCCAT CAACGGTAAT 
CCAGTCCATG AAAATTCCTT AGCCAGCACA GAGAACCAGT TACAACACAC AAGCTCCTTG 
CTTCAAACCG CCGAACAGAC CCTTCCTGTG ATTGGCCACG CTTCTCTTCC TGCTGTTGAC 
CACACACTCT CTGTGAGCAG TGACTCGGGC AACTCCACTG CATCCATTAA AACTGATCGT 
ACCGACGAGC ACAGCCAGCC AGTGCACAGC ACTGTCAACC ACAACAATCC GGCAGTTCCC 
CACCCGACAC TTAGTCCCCA GGAGAAAAAA GAGCTTGATC AGCTCCTTAG TGGCCTCGAA 
GCACCGACTC TTCAACGACA AGCCTACCTG GCTACATCCA CCAGTCCGGG AGGCGGTGTC 
CGACACCTGG TCCCAGCACA AGTGCACGTT AACGGAGGCC ACCCAAGGCT AGTTCCTGTC 
CCCTCAACGG AAGAGCGTGA GACGGACATT CTTGACGACG AGCTGCCAAA TAGCCAAGAG 
GGAAACAGTG TGGACAGCCT GGGTACACTG TCATCCTTAG AGGGTCAAGC AACCCCAGGG 
GATCTCTATT ACCAACAAGA GAAGTCCGTC AGCAGACAAA ACGACGGGCC ATATCTTGAG 
AGAGGCGTTT GTCGGGAAAA AAAAAACGAG ATGCCCATGC ATGGAGTTCA TACTCCGATG 
ATGCAGGAAA GACCTGTGGA CTCTGTATCC CCACAAGGGG GATATAACAA ATACCAGAAC 
GGTGGTGGGA TGTACCACTC TGAATCCTAT GGGATTCCAC CTGCCAGCAG CCCGGAGGGA 
AACCCTAAAC TGATGCCCAG GGCCCCAGAG AGAAGCACAA GTAGCCGGGA GGCTGTTCAA 
AGGGGCCTCA ATGCCTGGCA CCAGTATAGC CTCCCAGATG ATCCATTTGG ACCTCCCCTG 
CAGTCAACCC ATAGCATGCC ACAGTTCCCC ACAGCAGCCT CACAGCGGGA CATAGAGCAG 
TCCATCGAGG CACTGAATAT GCTCATGCTT GACCTGGACC CCATTAACTC TCACATGTCC 
AAGTCCCATA GTGCACCCTC CGGGGAGAAC ATCCTGAATT CATCCCAGGC ACCCTTCTCC 
CAGACCCTCT CCAGACCCTC ATACCAAGGA GACTCCGCCA TCCACAGATA CAATAACTCT 
GAATCTGTCA ACAATCCCTC CCATCAGCCC ATATGGTCAG CTGGAAGAGT GTCAGCAGTG 
CCTAACCAGA GCCCTATTAT GGATTCTCAA GCACATTTGC CCCAGAGATC CAGCTCGAGC 
TGTCAACACC AGAAAACCAC CCCAACACAC ACCCCAGAGC CCAATCTCCA TGCACGACAA 
GGAGCCATCC ATTACCCCAT TGATCCCTCC TTTAATTTCA ACCAGCATCT CCCAGTTAGG 
CCTGCAAACA CTTTCCCAGG TGTTTCCCCC TCCCCAGACC TGCAGGGTTC GTCTCCATAC 
CAGGGCTTCA GTGCCTCTTC CTCACCCCTT CCTGCGCTCA CTCCACAGCC AAAGAACACA 
TCGTCTTCAT CCCTGCCTCA AGAACAAGAT GTTGAGGAAG AGGCGCTGAA CTTGGAAGGT 
CTGGTGGCTC ATCGCATTGC CGGCGTTCGC TCCAGAGGAA TGACCCCAGA AGTGACACAG 
GAGAGCGGTC GACGTAGGAC CACCAGTGAG GGACAGTACC AGAGCAGCCA CGACAGCACA 
CCAATGGTTG ATTCACCGGA CTTTGCCCAC AGTTTGACCC TCAACCCTGG AGGCAGACCC 
AGAGAGGGCC AGATTCACAG CTACAGGGAG GCGTTGGAGG ACAGCGAGGT GGACCTTGTC 
ATGCACAGTC CCACCTTCAG AGGCGGTGGT GAGAATTCCC CTCAGACCCC CAAGTTCCCT 
GTGTCACCAC AGACTCCTTA CTTTAACATG TCTCGTGTCC CTCCAGGTCT GGCCAAGACT 
CCTCTGTCAG CACTGGAACT GAAGCCCCAT GATCACGACT CTGATTCAAA TGAGGGAGAG 
CAAGATAACC ACACTTCCAG GGACTCAAGA GTTCAACCCT TCAACACCTC TTTCTCTTCC 
ACCTACCCTA GTCACAGCAG TGATGGAAGA AGAACCGGTT CTTCAGACGT TCACAGCCAC 
ACCCCCTCCT ACGCAAACAG ACCCACCTCC GCTGAGGGCT CCCAGGTCAA CGTCATGGGA 
GTCCACACAG TGCCCGGCAG TCCCAACACC CTCCATCGCA CCGTAGCCAC CAACACTCCA 
CCAAGCCCGG CCCTTCAGAG ACGTCTGGGT CAAGCCAGTC CCTCACTAGC CAGACACCAA 
CCCCCAGCTG GTGCCAACAG GGACAGTGTC CCCTCCAGTC CTCTGATTGG CCGCAACCCT 
AAAACAGCAG CTGCTGGCGT GCCCCCCAGC CCTCTAATGG GCCGCCGAAC GGCGATGAGC 
GGCCACAGCA CGCCTGATGA CATCGGCCCA CCACCTTCTA CGCCTGCCTT CCCTGTGTCC 
CCACAGCTAC CAGAAAAGAG GCACATGTCC ACTGGAGACG TGGAGAGGAC AGATAACAAG 
AACCTTATGG CCGGTGTTGG CAGCAGTGCG CCAAACCTGT CTGGCACGCA CACGTTGACA 
GACGTCCAAA AGTCCATCTA TGATGGGTAT CCGGACATTA AGATGAATGT GAAGTTTGTC 
CAAGACACAT CCAAGTTCTG GTACAAGCCA GACATCTCCA GAGAGCAAGC CATCAATCTG 
TTGAAGGACA GAGAGCCCGG GGCCTTCATC ATAAGGGACA GCCATTCCTT CCGTGGAGCT 
TACGGCCTTG CAATGAAGGT GGCCTGCCCG CCGCCAACTG TCCAGCAGAA CAAAAAAGTT 
GGAGACTTGA CCAACGAGCT TGTGAGACAC TTCCTGATTG AGACAAGCTC CAGAGGTGTT 
CGACTCAAAG GCTGTCCCAA TGAACCCTAC TTTGGTTGCC TCTCAGCGCT GGTTTATCAA 
CACTCAATGA CCCCGCTAGC TCTGCCCTGT AAGCTGATGA TCCCCACCAA AGACCCGAAT 
GAGGAAGCGC TGGAGTTGGC CACACCCACT GACCCAGTTG TCGAGCTTCT GAAGCAAGGA 
GCAGTTCAGA AGGTTCCAGA GGAGGCTTAC GCGTGTAACG TCCTCTACAT CAACTCGGTG 
GACATGGAGT CTCTCACGGG GCCCCAGGCC ATCGCCAAGG CGATCAGCCT GACTTTGGCG 
ACAAACCCCC TGCCGGCCGC CACCACCGTC CACTTCAAAG TGTCTATGCA GGGCATCACG 
CTCACCGACA GCCAGAGGAA ACTTTTTTTC AGACGCCACT ATCCCATCAA CACCATAACG 
TTCTGTGACA TTGACCCGCA GAACAGGAAA TGGGGAAAAG AAGGAGGCGG TTCAGTGAAG 
CTGTTTGGTT TTGTGGCACG GAAACAAGGA AGCACAACCG ACAACGTGAG CCACCTGTTC 
GCAGAGCTGG ACCCAGATCA GCCGGCTTCC GCCATCACCA GTTTTGTCTC CAAGACGATG 
AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840189.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696049.1 Takifugu rubripes tensin (LOC101064247), transcript variant X1, mRNA.

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CloneID	OTo71053
Clone ID Related Accession (Same CDS sequence)	XM_029840189.1
Accession Version	XM_029840189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGAGTACA ACGCTCGTAT CCGGGGCATC AGCGAAAGCA TGACACCGCA ACAATCTGAC 
CGTCACCGCT CCTATTCCTT CTCTGGCGTT CGCTCCAGAG GAATGACCCC AGAAGTGACA 
CAGGACAGCG GTCGACGTAG GACCACCAGT GAGGGACAGT ACCAGAGCAG CCACGACAGC 
ACACCAATGG TTGATTCACC GGACTTTGCC CACAGTTTGA CCCTCAACCC TGGAGGCAGA 
CCCAGAGAGG GCCAGATTCA CAGCTACAGG GAGGTGTTGG AGGACAGCGA GGTGGACCTT 
GTCATGCACA GTCCCACCTT CAGAGGCGGT GGTGAGAATT CCCCTCAGAC CCCCAAGTTC 
CCTGTGTCAC CACAGACTCC TTACTTTAAC ATGTCTCGTG TCCCTCCAGG TCTGGCCAAG 
ACTCCTCTGT CAGCACTGGA ACTGAAGCCC CATGATCACG ACTCTGATTC AAATGAGGGA 
GAGCAAGATA ACCACACTTC CAGGGACTCA AGAGTTCAAC CCTTCAACAC CTCTTTCTCT 
TCCACCTACC CTAGTCACAG CAGTGATGGA AGAAGAACCG GTTCTTCAGA CGTTCACAGC 
CACACCCCCT CCTACGCAAA CAGACCCACC TCCGCTGAGG GCTCCCAGGT CAACGTCATG 
GGAGTCCACA CAGTGCCCGG CAGTCCCAAC ACCCTCCATC GCACCGTAGC CACCAACACT 
CCACCAAGCC CGGCCCTTCA GAGACGTCTG GGTCAAGCCA GTCCCTCACT AGCCAGACAC 
CAACCCCCAG CTGGTGCCAA CAGGGACAGT GTCCCCTCCA GTCCTCTGAT TGGCCGCAAC 
CCTAAAACAG CAGCTGCTGG CGTGCCCCCC AGCCCTCTAA TGGGCCGCCG AACGGCGATG 
AGCGGCCACA GCACGCCTGA TGACATCGGC CCACCACCTT CTACGCCTGC CTTCCCTGTG 
TCCCCACAGC TACCAGAAAA GAGGCACATG TCCACTGGAG ACGTGGAGAG GACAGATAAC 
AAGAACCTTA TGGCCGGTGT TGGCAGCAGT GCGCCAAACC TGTCTGGCAC GCACACGTTG 
ACAGACGTCC AAAAGTCCAT CTATGATGGG TATCCGGACA TTAAGATGAA TGTGAAGTTT 
GTCCAAGACA CATCCAAGTT CTGGTACAAG CCAGACATCT CCAGAGAGCA AGCCATCAAT 
CTGTTGAAGG ACAGAGAGCC CGGGGCCTTC ATCATAAGGG ACAGCCATTC CTTCCGTGGA 
GCTTACGGCC TTGCAATGAA GGTGGCCTGC CCGCCGCCAA CTGTCCAGCA GAACAAAAAA 
GTTGGAGACT TGACCAACGA GCTTGTGAGA CACTTCCTGA TTGAGACAAG CTCCAGAGGT 
GTTCGACTCA AAGGCTGTCC CAATGAACCC TACTTTGGTT GCCTCTCAGC GCTGGTTTAT 
CAACACTCAA TGACCCCGCT AGCTCTGCCC TGTAAGCTGA TGATCCCCAC CAAAGACCCG 
AATGAGGAAG CGCTGGAGTT GGCCACACCC ACTGACCCAG TTGTCGAGCT TCTGAAGCAA 
GGAGCAGTTC AGAAGGTTCC AGAGGAGGCT TACGCGTGTA ACGTCCTCTA CATCAACTCG 
GTGGACATGG AGTCTCTCAC GGGGCCCCAG GCCATCGCCA AGGCGATCAG CCTGACTTTG 
GCGACAAACC CCCTGCCGGC CGCCACCACC GTCCACTTCA AAGTGTCTAT GCAGGGCATC 
ACGCTCACCG ACAGCCAGAG GAAACTTTTT TTCAGACGCC ACTATCCCAT CAACACCATA 
ACGTTCTGTG ACATTGACCC GCAGAACAGG AAATGGGGAA AAGAAGGAGG CGGTTCAGTG 
AAGCTGTTTG GTTTTGTGGC ACGGAAACAA GGAAGCACAA CCGACAACGT GAGCCACCTG 
TTCGCAGAGC TGGACCCAGA TCAGCCGGCT TCCGCCATCA CCAGTTTTGT CTCCAAGACG 
ATGAAGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696049.1
CDS	646..5757
Translation	MARLNWCLAAVISWGKFLFACFFPLRGAKRDTPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELPPTPQLPLKHVDTPGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840189.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840189.1

ORF Insert Sequence:

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGAGTACA ACGCTCGTAT CCGGGGCATC AGCGAAAGCA TGACACCGCA ACAATCTGAC 
CGTCACCGCT CCTATTCCTT CTCTGGCGTT CGCTCCAGAG GAATGACCCC AGAAGTGACA 
CAGGACAGCG GTCGACGTAG GACCACCAGT GAGGGACAGT ACCAGAGCAG CCACGACAGC 
ACACCAATGG TTGATTCACC GGACTTTGCC CACAGTTTGA CCCTCAACCC TGGAGGCAGA 
CCCAGAGAGG GCCAGATTCA CAGCTACAGG GAGGTGTTGG AGGACAGCGA GGTGGACCTT 
GTCATGCACA GTCCCACCTT CAGAGGCGGT GGTGAGAATT CCCCTCAGAC CCCCAAGTTC 
CCTGTGTCAC CACAGACTCC TTACTTTAAC ATGTCTCGTG TCCCTCCAGG TCTGGCCAAG 
ACTCCTCTGT CAGCACTGGA ACTGAAGCCC CATGATCACG ACTCTGATTC AAATGAGGGA 
GAGCAAGATA ACCACACTTC CAGGGACTCA AGAGTTCAAC CCTTCAACAC CTCTTTCTCT 
TCCACCTACC CTAGTCACAG CAGTGATGGA AGAAGAACCG GTTCTTCAGA CGTTCACAGC 
CACACCCCCT CCTACGCAAA CAGACCCACC TCCGCTGAGG GCTCCCAGGT CAACGTCATG 
GGAGTCCACA CAGTGCCCGG CAGTCCCAAC ACCCTCCATC GCACCGTAGC CACCAACACT 
CCACCAAGCC CGGCCCTTCA GAGACGTCTG GGTCAAGCCA GTCCCTCACT AGCCAGACAC 
CAACCCCCAG CTGGTGCCAA CAGGGACAGT GTCCCCTCCA GTCCTCTGAT TGGCCGCAAC 
CCTAAAACAG CAGCTGCTGG CGTGCCCCCC AGCCCTCTAA TGGGCCGCCG AACGGCGATG 
AGCGGCCACA GCACGCCTGA TGACATCGGC CCACCACCTT CTACGCCTGC CTTCCCTGTG 
TCCCCACAGC TACCAGAAAA GAGGCACATG TCCACTGGAG ACGTGGAGAG GACAGATAAC 
AAGAACCTTA TGGCCGGTGT TGGCAGCAGT GCGCCAAACC TGTCTGGCAC GCACACGTTG 
ACAGACGTCC AAAAGTCCAT CTATGATGGG TATCCGGACA TTAAGATGAA TGTGAAGTTT 
GTCCAAGACA CATCCAAGTT CTGGTACAAG CCAGACATCT CCAGAGAGCA AGCCATCAAT 
CTGTTGAAGG ACAGAGAGCC CGGGGCCTTC ATCATAAGGG ACAGCCATTC CTTCCGTGGA 
GCTTACGGCC TTGCAATGAA GGTGGCCTGC CCGCCGCCAA CTGTCCAGCA GAACAAAAAA 
GTTGGAGACT TGACCAACGA GCTTGTGAGA CACTTCCTGA TTGAGACAAG CTCCAGAGGT 
GTTCGACTCA AAGGCTGTCC CAATGAACCC TACTTTGGTT GCCTCTCAGC GCTGGTTTAT 
CAACACTCAA TGACCCCGCT AGCTCTGCCC TGTAAGCTGA TGATCCCCAC CAAAGACCCG 
AATGAGGAAG CGCTGGAGTT GGCCACACCC ACTGACCCAG TTGTCGAGCT TCTGAAGCAA 
GGAGCAGTTC AGAAGGTTCC AGAGGAGGCT TACGCGTGTA ACGTCCTCTA CATCAACTCG 
GTGGACATGG AGTCTCTCAC GGGGCCCCAG GCCATCGCCA AGGCGATCAG CCTGACTTTG 
GCGACAAACC CCCTGCCGGC CGCCACCACC GTCCACTTCA AAGTGTCTAT GCAGGGCATC 
ACGCTCACCG ACAGCCAGAG GAAACTTTTT TTCAGACGCC ACTATCCCAT CAACACCATA 
ACGTTCTGTG ACATTGACCC GCAGAACAGG AAATGGGGAA AAGAAGGAGG CGGTTCAGTG 
AAGCTGTTTG GTTTTGTGGC ACGGAAACAA GGAAGCACAA CCGACAACGT GAGCCACCTG 
TTCGCAGAGC TGGACCCAGA TCAGCCGGCT TCCGCCATCA CCAGTTTTGT CTCCAAGACG 
ATGAAGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840190.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696050.1 Takifugu rubripes tensin (LOC101064247), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo71054
Clone ID Related Accession (Same CDS sequence)	XM_029840190.1
Accession Version	XM_029840190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG TCTCTCTGTC GTTGCCCTCC ACCCTGGCTG GGAGGACCAG GACCTGGGTT 
TGCCTCTCCT GCATGTTCTG GCCGGATGAG CTGGAGGGCG TCCACACTCA CACCTTCAAG 
CTGAAGCCAT TCAAGAAGGC CAAAAGCTGT GACATCTGTA AGCAGGCCAT CACCAAGGAG 
GGCCTCGTCT GCAAAGCGTG TCGGCTCTCC TGCCACAAAA AATGTGAAGT GAAGGTCACC 
ACATCATGTC AGACGTCCAC CAACCATGAG CTGCCCCCCA CTCCGCAGCT GCCATTAAAG 
CATGTAGACA CGCCGGGATC TGCGAGGTCC TGCAAGAGTG TGGAGATAAG GCGGAAGCAG 
TCTCGAAGCC AGAGTGTGGT TCTGACCATG GAGGAGAACT ATGAGGTAGA CCTGGTCTAC 
ATCACGGAGA GGATCATCTC CCTCTGCTTC CCCATTGGCA TGGAGGACCA GGGCTACATC 
GCCAACCTCA AGGAGGTGGC CACCATGCTG CGATCCAAAC ATGGAGATCA TTATTTGGTG 
CTGAACCTGA GTGAATGGAG GACTGAGTTA TCGAAGCTAA ACCACAAGGT CTTGGAATTT 
GGCTGGCCGG ACTTTCACGC ACCAGCCCTG GACAAGATCT GTAGCATGTG CAAAGCGATT 
GACACGTGGC TCAATGGAGA CTCGCACAAT GTGGTGGTGC TTCACAACAA GGGGAACCGA 
GGTCGAACTG GGGTCGTGGT GGCTGCTTAC ATGCACTATA GCAACATATC AGCAAGTGCT 
GACCAGGCCT TGGACAGGTT CGCCATGAGA CGGTTCTACG AGGATAAAGC ACTTCCTGTG 
GGTCAGCCGT CACAAAAAAG GTATGTGCGA TACTTCAATG GCCTTCTGTC GGGGCACATC 
AAAATCAACA ACAAACCTCT CTTCCTGCAC CACGTCATCA TGCACGGTAT CCCCAACTTT 
GAGTCTAAAG GAGGCTGCCG CCCTTTCCTG AAGATCTACC AAGCGATGCA GCCAGTCTAC 
ACGTCAGGGA TATACAACGT GCAGGGAGAC AGTAACACTA GTATCTGCAT CACCATCGAA 
CCAGGCCTCC TCCTGAAGGG AGACATCCTG CTGAAGTGTT ACCACAAACG ATACAGAGGC 
CCGACCAGAG ACGTAGTGTT CCGGGTGCAG TTCCACACCT GTGCCATCCA CGACCTTGGC 
GTGGTGTTCG GGAAGAGCGA ACTGGACGAG ACCTTCAAAG ACGACAGGTT TCCTGAATAC 
GGGAAAGTGG AGTTTGTTTT CTCCTATGCA CCAGAAAAAA TTAGAGGCTT AGACCACCTG 
GAGAACGGCC CCAGTGTTTC CGTGGACTAC AACACCCAGG AAAACCTGAT TCGCTGTGAT 
TCATATGACA ACTTTAGCCA GCGCTCTGAA GATGCCGTGG ATGGAGAGCG TGAGGTCATT 
CACACTCAAG GTCCACTTGA TGGAAGCCTT TATGCCCGGG TCTGTAAGAA AGACTCCCTG 
GAGGGAGTTG TCGCCATCAA CGGTAATCCA GTCCATGAAA ATTCCTTAGC CAGCACAGAG 
AACCAGTTAC AACACACAAG CTCCTTGCTT CAAACCGCCG AACAGACCCT TCCTGTGATT 
GGCCACGCTT CTCTTCCTGC TGTTGACCAC ACACTCTCTG TGAGCAGTGA CTCGGGCAAC 
TCCACTGCAT CCATTAAAAC TGATCGTACC GACGAGCACA GCCAGCCAGT GCACAGCACT 
GTCAACCACA ACAATCCGGC AGTTCCCCAC CCGACACTTA GTCCCCAGGA GAAAAAAGAG 
CTTGATCAGC TCCTTAGTGG CCTCGAAGCA CCGACTCTTC AACGACAAGC CTACCTGGCT 
ACATCCACCA GTCCGGGAGG CGGTGTCCGA CACCTGGTCC CAGCACAAGT GCACGTTAAC 
GGAGGCCACC CAAGGCTAGT TCCTGTCCCC TCAACGGAAG AGCGTGAGAC GGACATTCTT 
GACGACGAGC TGCCAAATAG CCAAGAGGGA AACAGTGTGG ACAGCCTGGG TACACTGTCA 
TCCTTAGAGG GTCAAGCAAC CCCAGGGGAT CTCTATTACC AACAAGAGAA GTCCGTCAGC 
AGACAAAACG ACGGGCCATA TCTTGAGAGA GGCGTTTGTC GGGAAAAAAA AAACGAGATG 
CCCATGCATG GAGTTCATAC TCCGATGATG CAGGAAAGAC CTGTGGACTC TGTATCCCCA 
CAAGGGGGAT ATAACAAATA CCAGAACGGT GGTGGGATGT ACCACTCTGA ATCCTATGGG 
ATTCCACCTG CCAGCAGCCC GGAGGGAAAC CCTAAACTGA TGCCCAGGGC CCCAGAGAGA 
AGCACAAGTA GCCGGGAGGC TGTTCAAAGG GGCCTCAATG CCTGGCACCA GTATAGCCTC 
CCAGATGATC CATTTGGACC TCCCCTGCAG TCAACCCATA GCATGCCACA GTTCCCCACA 
GCAGCCTCAC AGCGGGACAT AGAGCAGTCC ATCGAGGCAC TGAATATGCT CATGCTTGAC 
CTGGACCCCA TTAACTCTCA CATGTCCAAG TCCCATAGTG CACCCTCCGG GGAGAACATC 
CTGAATTCAT CCCAGGCACC CTTCTCCCAG ACCCTCTCCA GACCCTCATA CCAAGGAGAC 
TCCGCCATCC ACAGATACAA TAACTCTGAA TCTGTCAACA ATCCCTCCCA TCAGCCCATA 
TGGTCAGCTG GAAGAGTGTC AGCAGTGCCT AACCAGAGCC CTATTATGGA TTCTCAAGCA 
CATTTGCCCC AGAGATCCAG CTCGAGCTGT CAACACCAGA AAACCACCCC AACACACACC 
CCAGAGCCCA ATCTCCATGC ACGACAAGGA GCCATCCATT ACCCCATTGA TCCCTCCTTT 
AATTTCAACC AGCATCTCCC AGTTAGGCCT GCAAACACTT TCCCAGGTGT TTCCCCCTCC 
CCAGACCTGC AGGGTTCGTC TCCATACCAG GGCTTCAGTG CCTCTTCCTC ACCCCTTCCT 
GCGCTCACTC CACAGCCAAA GAACACATCG TCTTCATCCC TGCCTCAAGA ACAAGATGTT 
GAGGAAGAGG CGCTGAACTT GGAAGGTCTG GTGGCTCATC GCATTGCCGA GTACAACGCT 
CGTATCCGGG GCATCAGCGA AAGCATGACA CCGCAACAAT CTGACCGTCA CCGCTCCTAT 
TCCTTCTCTG GCGTTCGCTC CAGAGGAATG ACCCCAGAAG TGACACAGGA CAGCGGTCGA 
CGTAGGACCA CCAGTGAGGG ACAGTACCAG AGCAGCCACG ACAGCACACC AATGGTTGAT 
TCACCGGACT TTGCCCACAG TTTGACCCTC AACCCTGGAG GCAGACCCAG AGAGGGCCAG 
ATTCACAGCT ACAGGGAGGT GTTGGAGGAC AGCGAGGTGG ACCTTGTCAT GCACAGTCCC 
ACCTTCAGAG GCGGTGGTGA GAATTCCCCT CAGACCCCCA AGTTCCCTGT GTCACCACAG 
ACTCCTTACT TTAACATGTC TCGTGTCCCT CCAGGTCTGG CCAAGACTCC TCTGTCAGCA 
CTGGAACTGA AGCCCCATGA TCACGACTCT GATTCAAATG AGGGAGAGCA AGATAACCAC 
ACTTCCAGGG ACTCAAGAGT TCAACCCTTC AACACCTCTT TCTCTTCCAC CTACCCTAGT 
CACAGCAGTG ATGGAAGAAG AACCGGTTCT TCAGACGTTC ACAGCCACAC CCCCTCCTAC 
GCAAACAGAC CCACCTCCGC TGAGGGCTCC CAGGTCAACG TCATGGGAGT CCACACAGTG 
CCCGGCAGTC CCAACACCCT CCATCGCACC GTAGCCACCA ACACTCCACC AAGCCCGGCC 
CTTCAGAGAC GTCTGGGTCA AGCCAGTCCC TCACTAGCCA GACACCAACC CCCAGCTGGT 
GCCAACAGGG ACAGTGTCCC CTCCAGTCCT CTGATTGGCC GCAACCCTAA AACAGCAGCT 
GCTGGCGTGC CCCCCAGCCC TCTAATGGGC CGCCGAACGG CGATGAGCGG CCACAGCACG 
CCTGATGACA TCGGCCCACC ACCTTCTACG CCTGCCTTCC CTGTGTCCCC ACAGCTACCA 
GAAAAGAGGC ACATGTCCAC TGGAGACGTG GAGAGGACAG ATAACAAGAA CCTTATGGCC 
GGTGTTGGCA GCAGTGCGCC AAACCTGTCT GGCACGCACA CGTTGACAGA CGTCCAAAAG 
TCCATCTATG ATGGGTATCC GGACATTAAG ATGAATGTGA AGTTTGTCCA AGACACATCC 
AAGTTCTGGT ACAAGCCAGA CATCTCCAGA GAGCAAGCCA TCAATCTGTT GAAGGACAGA 
GAGCCCGGGG CCTTCATCAT AAGGGACAGC CATTCCTTCC GTGGAGCTTA CGGCCTTGCA 
ATGAAGGTGG CCTGCCCGCC GCCAACTGTC CAGCAGAACA AAAAAGTTGG AGACTTGACC 
AACGAGCTTG TGAGACACTT CCTGATTGAG ACAAGCTCCA GAGGTGTTCG ACTCAAAGGC 
TGTCCCAATG AACCCTACTT TGGTTGCCTC TCAGCGCTGG TTTATCAACA CTCAATGACC 
CCGCTAGCTC TGCCCTGTAA GCTGATGATC CCCACCAAAG ACCCGAATGA GGAAGCGCTG 
GAGTTGGCCA CACCCACTGA CCCAGTTGTC GAGCTTCTGA AGCAAGGAGC AGTTCAGAAG 
GTTCCAGAGG AGGCTTACGC GTGTAACGTC CTCTACATCA ACTCGGTGGA CATGGAGTCT 
CTCACGGGGC CCCAGGCCAT CGCCAAGGCG ATCAGCCTGA CTTTGGCGAC AAACCCCCTG 
CCGGCCGCCA CCACCGTCCA CTTCAAAGTG TCTATGCAGG GCATCACGCT CACCGACAGC 
CAGAGGAAAC TTTTTTTCAG ACGCCACTAT CCCATCAACA CCATAACGTT CTGTGACATT 
GACCCGCAGA ACAGGAAATG GGGAAAAGAA GGAGGCGGTT CAGTGAAGCT GTTTGGTTTT 
GTGGCACGGA AACAAGGAAG CACAACCGAC AACGTGAGCC ACCTGTTCGC AGAGCTGGAC 
CCAGATCAGC CGGCTTCCGC CATCACCAGT TTTGTCTCCA AGACGATGAA GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696050.1
CDS	161..5257
Translation	MPSVSLSLPSTLAGRTRTWVCLSCMFWPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELPPTPQLPLKHVDTPGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840190.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840190.1

ORF Insert Sequence:

ATGCCCTCGG TCTCTCTGTC GTTGCCCTCC ACCCTGGCTG GGAGGACCAG GACCTGGGTT 
TGCCTCTCCT GCATGTTCTG GCCGGATGAG CTGGAGGGCG TCCACACTCA CACCTTCAAG 
CTGAAGCCAT TCAAGAAGGC CAAAAGCTGT GACATCTGTA AGCAGGCCAT CACCAAGGAG 
GGCCTCGTCT GCAAAGCGTG TCGGCTCTCC TGCCACAAAA AATGTGAAGT GAAGGTCACC 
ACATCATGTC AGACGTCCAC CAACCATGAG CTGCCCCCCA CTCCGCAGCT GCCATTAAAG 
CATGTAGACA CGCCGGGATC TGCGAGGTCC TGCAAGAGTG TGGAGATAAG GCGGAAGCAG 
TCTCGAAGCC AGAGTGTGGT TCTGACCATG GAGGAGAACT ATGAGGTAGA CCTGGTCTAC 
ATCACGGAGA GGATCATCTC CCTCTGCTTC CCCATTGGCA TGGAGGACCA GGGCTACATC 
GCCAACCTCA AGGAGGTGGC CACCATGCTG CGATCCAAAC ATGGAGATCA TTATTTGGTG 
CTGAACCTGA GTGAATGGAG GACTGAGTTA TCGAAGCTAA ACCACAAGGT CTTGGAATTT 
GGCTGGCCGG ACTTTCACGC ACCAGCCCTG GACAAGATCT GTAGCATGTG CAAAGCGATT 
GACACGTGGC TCAATGGAGA CTCGCACAAT GTGGTGGTGC TTCACAACAA GGGGAACCGA 
GGTCGAACTG GGGTCGTGGT GGCTGCTTAC ATGCACTATA GCAACATATC AGCAAGTGCT 
GACCAGGCCT TGGACAGGTT CGCCATGAGA CGGTTCTACG AGGATAAAGC ACTTCCTGTG 
GGTCAGCCGT CACAAAAAAG GTATGTGCGA TACTTCAATG GCCTTCTGTC GGGGCACATC 
AAAATCAACA ACAAACCTCT CTTCCTGCAC CACGTCATCA TGCACGGTAT CCCCAACTTT 
GAGTCTAAAG GAGGCTGCCG CCCTTTCCTG AAGATCTACC AAGCGATGCA GCCAGTCTAC 
ACGTCAGGGA TATACAACGT GCAGGGAGAC AGTAACACTA GTATCTGCAT CACCATCGAA 
CCAGGCCTCC TCCTGAAGGG AGACATCCTG CTGAAGTGTT ACCACAAACG ATACAGAGGC 
CCGACCAGAG ACGTAGTGTT CCGGGTGCAG TTCCACACCT GTGCCATCCA CGACCTTGGC 
GTGGTGTTCG GGAAGAGCGA ACTGGACGAG ACCTTCAAAG ACGACAGGTT TCCTGAATAC 
GGGAAAGTGG AGTTTGTTTT CTCCTATGCA CCAGAAAAAA TTAGAGGCTT AGACCACCTG 
GAGAACGGCC CCAGTGTTTC CGTGGACTAC AACACCCAGG AAAACCTGAT TCGCTGTGAT 
TCATATGACA ACTTTAGCCA GCGCTCTGAA GATGCCGTGG ATGGAGAGCG TGAGGTCATT 
CACACTCAAG GTCCACTTGA TGGAAGCCTT TATGCCCGGG TCTGTAAGAA AGACTCCCTG 
GAGGGAGTTG TCGCCATCAA CGGTAATCCA GTCCATGAAA ATTCCTTAGC CAGCACAGAG 
AACCAGTTAC AACACACAAG CTCCTTGCTT CAAACCGCCG AACAGACCCT TCCTGTGATT 
GGCCACGCTT CTCTTCCTGC TGTTGACCAC ACACTCTCTG TGAGCAGTGA CTCGGGCAAC 
TCCACTGCAT CCATTAAAAC TGATCGTACC GACGAGCACA GCCAGCCAGT GCACAGCACT 
GTCAACCACA ACAATCCGGC AGTTCCCCAC CCGACACTTA GTCCCCAGGA GAAAAAAGAG 
CTTGATCAGC TCCTTAGTGG CCTCGAAGCA CCGACTCTTC AACGACAAGC CTACCTGGCT 
ACATCCACCA GTCCGGGAGG CGGTGTCCGA CACCTGGTCC CAGCACAAGT GCACGTTAAC 
GGAGGCCACC CAAGGCTAGT TCCTGTCCCC TCAACGGAAG AGCGTGAGAC GGACATTCTT 
GACGACGAGC TGCCAAATAG CCAAGAGGGA AACAGTGTGG ACAGCCTGGG TACACTGTCA 
TCCTTAGAGG GTCAAGCAAC CCCAGGGGAT CTCTATTACC AACAAGAGAA GTCCGTCAGC 
AGACAAAACG ACGGGCCATA TCTTGAGAGA GGCGTTTGTC GGGAAAAAAA AAACGAGATG 
CCCATGCATG GAGTTCATAC TCCGATGATG CAGGAAAGAC CTGTGGACTC TGTATCCCCA 
CAAGGGGGAT ATAACAAATA CCAGAACGGT GGTGGGATGT ACCACTCTGA ATCCTATGGG 
ATTCCACCTG CCAGCAGCCC GGAGGGAAAC CCTAAACTGA TGCCCAGGGC CCCAGAGAGA 
AGCACAAGTA GCCGGGAGGC TGTTCAAAGG GGCCTCAATG CCTGGCACCA GTATAGCCTC 
CCAGATGATC CATTTGGACC TCCCCTGCAG TCAACCCATA GCATGCCACA GTTCCCCACA 
GCAGCCTCAC AGCGGGACAT AGAGCAGTCC ATCGAGGCAC TGAATATGCT CATGCTTGAC 
CTGGACCCCA TTAACTCTCA CATGTCCAAG TCCCATAGTG CACCCTCCGG GGAGAACATC 
CTGAATTCAT CCCAGGCACC CTTCTCCCAG ACCCTCTCCA GACCCTCATA CCAAGGAGAC 
TCCGCCATCC ACAGATACAA TAACTCTGAA TCTGTCAACA ATCCCTCCCA TCAGCCCATA 
TGGTCAGCTG GAAGAGTGTC AGCAGTGCCT AACCAGAGCC CTATTATGGA TTCTCAAGCA 
CATTTGCCCC AGAGATCCAG CTCGAGCTGT CAACACCAGA AAACCACCCC AACACACACC 
CCAGAGCCCA ATCTCCATGC ACGACAAGGA GCCATCCATT ACCCCATTGA TCCCTCCTTT 
AATTTCAACC AGCATCTCCC AGTTAGGCCT GCAAACACTT TCCCAGGTGT TTCCCCCTCC 
CCAGACCTGC AGGGTTCGTC TCCATACCAG GGCTTCAGTG CCTCTTCCTC ACCCCTTCCT 
GCGCTCACTC CACAGCCAAA GAACACATCG TCTTCATCCC TGCCTCAAGA ACAAGATGTT 
GAGGAAGAGG CGCTGAACTT GGAAGGTCTG GTGGCTCATC GCATTGCCGA GTACAACGCT 
CGTATCCGGG GCATCAGCGA AAGCATGACA CCGCAACAAT CTGACCGTCA CCGCTCCTAT 
TCCTTCTCTG GCGTTCGCTC CAGAGGAATG ACCCCAGAAG TGACACAGGA CAGCGGTCGA 
CGTAGGACCA CCAGTGAGGG ACAGTACCAG AGCAGCCACG ACAGCACACC AATGGTTGAT 
TCACCGGACT TTGCCCACAG TTTGACCCTC AACCCTGGAG GCAGACCCAG AGAGGGCCAG 
ATTCACAGCT ACAGGGAGGT GTTGGAGGAC AGCGAGGTGG ACCTTGTCAT GCACAGTCCC 
ACCTTCAGAG GCGGTGGTGA GAATTCCCCT CAGACCCCCA AGTTCCCTGT GTCACCACAG 
ACTCCTTACT TTAACATGTC TCGTGTCCCT CCAGGTCTGG CCAAGACTCC TCTGTCAGCA 
CTGGAACTGA AGCCCCATGA TCACGACTCT GATTCAAATG AGGGAGAGCA AGATAACCAC 
ACTTCCAGGG ACTCAAGAGT TCAACCCTTC AACACCTCTT TCTCTTCCAC CTACCCTAGT 
CACAGCAGTG ATGGAAGAAG AACCGGTTCT TCAGACGTTC ACAGCCACAC CCCCTCCTAC 
GCAAACAGAC CCACCTCCGC TGAGGGCTCC CAGGTCAACG TCATGGGAGT CCACACAGTG 
CCCGGCAGTC CCAACACCCT CCATCGCACC GTAGCCACCA ACACTCCACC AAGCCCGGCC 
CTTCAGAGAC GTCTGGGTCA AGCCAGTCCC TCACTAGCCA GACACCAACC CCCAGCTGGT 
GCCAACAGGG ACAGTGTCCC CTCCAGTCCT CTGATTGGCC GCAACCCTAA AACAGCAGCT 
GCTGGCGTGC CCCCCAGCCC TCTAATGGGC CGCCGAACGG CGATGAGCGG CCACAGCACG 
CCTGATGACA TCGGCCCACC ACCTTCTACG CCTGCCTTCC CTGTGTCCCC ACAGCTACCA 
GAAAAGAGGC ACATGTCCAC TGGAGACGTG GAGAGGACAG ATAACAAGAA CCTTATGGCC 
GGTGTTGGCA GCAGTGCGCC AAACCTGTCT GGCACGCACA CGTTGACAGA CGTCCAAAAG 
TCCATCTATG ATGGGTATCC GGACATTAAG ATGAATGTGA AGTTTGTCCA AGACACATCC 
AAGTTCTGGT ACAAGCCAGA CATCTCCAGA GAGCAAGCCA TCAATCTGTT GAAGGACAGA 
GAGCCCGGGG CCTTCATCAT AAGGGACAGC CATTCCTTCC GTGGAGCTTA CGGCCTTGCA 
ATGAAGGTGG CCTGCCCGCC GCCAACTGTC CAGCAGAACA AAAAAGTTGG AGACTTGACC 
AACGAGCTTG TGAGACACTT CCTGATTGAG ACAAGCTCCA GAGGTGTTCG ACTCAAAGGC 
TGTCCCAATG AACCCTACTT TGGTTGCCTC TCAGCGCTGG TTTATCAACA CTCAATGACC 
CCGCTAGCTC TGCCCTGTAA GCTGATGATC CCCACCAAAG ACCCGAATGA GGAAGCGCTG 
GAGTTGGCCA CACCCACTGA CCCAGTTGTC GAGCTTCTGA AGCAAGGAGC AGTTCAGAAG 
GTTCCAGAGG AGGCTTACGC GTGTAACGTC CTCTACATCA ACTCGGTGGA CATGGAGTCT 
CTCACGGGGC CCCAGGCCAT CGCCAAGGCG ATCAGCCTGA CTTTGGCGAC AAACCCCCTG 
CCGGCCGCCA CCACCGTCCA CTTCAAAGTG TCTATGCAGG GCATCACGCT CACCGACAGC 
CAGAGGAAAC TTTTTTTCAG ACGCCACTAT CCCATCAACA CCATAACGTT CTGTGACATT 
GACCCGCAGA ACAGGAAATG GGGAAAAGAA GGAGGCGGTT CAGTGAAGCT GTTTGGTTTT 
GTGGCACGGA AACAAGGAAG CACAACCGAC AACGTGAGCC ACCTGTTCGC AGAGCTGGAC 
CCAGATCAGC CGGCTTCCGC CATCACCAGT TTTGTCTCCA AGACGATGAA GCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840191.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696051.1 Takifugu rubripes tensin (LOC101064247), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo71055
Clone ID Related Accession (Same CDS sequence)	XM_029840191.1
Accession Version	XM_029840191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGAGTACA ACGCTCGTAT CCGGGGCATC AGCGAAAGCA TGACACCGCA ACAATCTGAC 
CGTCACCGCT CCTATTCCTT CTCTGGCGTT CGCTCCAGAG GAATGACCCC AGAAGTGACA 
CAGGACAGCG GTCGACGTAG GACCACCAGT GAGGGACAGT ACCAGAGCAG CCACGACAGC 
ACACCAATGG TTGATTCACC GGACTTTGCC CACAGTTTGA CCCTCAACCC TGGAGGCAGA 
CCCAGAGAGG GCCAGATTCA CAGCTACAGG GAGGTGTTGG AGGACAGCGA GGTGGACCTT 
GTCATGCACA GTCCCACCTT CAGAGGCGGT GGTGAGAATT CCCCTCAGAC CCCCAAGTTC 
CCTGTGTCAC CACAGACTCC TTACTTTAAC ATGTCTCGTG TCCCTCCAGG TCTGGCCAAG 
ACTCCTCTGT CAGCACTGGA ACTGAAGCCC CATGATCACG ATAACCACAC TTCCAGGGAC 
TCAAGAGTTC AACCCTTCAA CACCTCTTTC TCTTCCACCT ACCCTAGTCA CAGCAGTGAT 
GGAAGAAGAA CCGGTTCTTC AGACGTTCAC AGCCACACCC CCTCCTACGC AAACAGACCC 
ACCTCCGCTG AGGGCTCCCA GGTCAACGTC ATGGGAGTCC ACACAGTGCC CGGCAGTCCC 
AACACCCTCC ATCGCACCGT AGCCACCAAC ACTCCACCAA GCCCGGCCCT TCAGAGACGT 
CTGGGTCAAG CCAGTCCCTC ACTAGCCAGA CACCAACCCC CAGCTGGTGC CAACAGGGAC 
AGTGTCCCCT CCAGTCCTCT GATTGGCCGC AACCCTAAAA CAGCAGCTGC TGGCGTGCCC 
CCCAGCCCTC TAATGGGCCG CCGAACGGCG ATGAGCGGCC ACAGCACGCC TGATGACATC 
GGCCCACCAC CTTCTACGCC TGCCTTCCCT GTGTCCCCAC AGCTACCAGA AAAGAGGCAC 
ATGTCCACTG GAGACGTGGA GAGGACAGAT AACAAGAACC TTATGGCCGG TGTTGGCAGC 
AGTGCGCCAA ACCTGTCTGG CACGCACACG TTGACAGACG TCCAAAAGTC CATCTATGAT 
GGGTATCCGG ACATTAAGAT GAATGTGAAG TTTGTCCAAG ACACATCCAA GTTCTGGTAC 
AAGCCAGACA TCTCCAGAGA GCAAGCCATC AATCTGTTGA AGGACAGAGA GCCCGGGGCC 
TTCATCATAA GGGACAGCCA TTCCTTCCGT GGAGCTTACG GCCTTGCAAT GAAGGTGGCC 
TGCCCGCCGC CAACTGTCCA GCAGAACAAA AAAGTTGGAG ACTTGACCAA CGAGCTTGTG 
AGACACTTCC TGATTGAGAC AAGCTCCAGA GGTGTTCGAC TCAAAGGCTG TCCCAATGAA 
CCCTACTTTG GTTGCCTCTC AGCGCTGGTT TATCAACACT CAATGACCCC GCTAGCTCTG 
CCCTGTAAGC TGATGATCCC CACCAAAGAC CCGAATGAGG AAGCGCTGGA GTTGGCCACA 
CCCACTGACC CAGTTGTCGA GCTTCTGAAG CAAGGAGCAG TTCAGAAGGT TCCAGAGGAG 
GCTTACGCGT GTAACGTCCT CTACATCAAC TCGGTGGACA TGGAGTCTCT CACGGGGCCC 
CAGGCCATCG CCAAGGCGAT CAGCCTGACT TTGGCGACAA ACCCCCTGCC GGCCGCCACC 
ACCGTCCACT TCAAAGTGTC TATGCAGGGC ATCACGCTCA CCGACAGCCA GAGGAAACTT 
TTTTTCAGAC GCCACTATCC CATCAACACC ATAACGTTCT GTGACATTGA CCCGCAGAAC 
AGGAAATGGG GAAAAGAAGG AGGCGGTTCA GTGAAGCTGT TTGGTTTTGT GGCACGGAAA 
CAAGGAAGCA CAACCGACAA CGTGAGCCAC CTGTTCGCAG AGCTGGACCC AGATCAGCCG 
GCTTCCGCCA TCACCAGTTT TGTCTCCAAG ACGATGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696051.1
CDS	646..5730
Translation	MARLNWCLAAVISWGKFLFACFFPLRGAKRDTPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELPPTPQLPLKHVDTPGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840191.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840191.1

ORF Insert Sequence:

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGAGTACA ACGCTCGTAT CCGGGGCATC AGCGAAAGCA TGACACCGCA ACAATCTGAC 
CGTCACCGCT CCTATTCCTT CTCTGGCGTT CGCTCCAGAG GAATGACCCC AGAAGTGACA 
CAGGACAGCG GTCGACGTAG GACCACCAGT GAGGGACAGT ACCAGAGCAG CCACGACAGC 
ACACCAATGG TTGATTCACC GGACTTTGCC CACAGTTTGA CCCTCAACCC TGGAGGCAGA 
CCCAGAGAGG GCCAGATTCA CAGCTACAGG GAGGTGTTGG AGGACAGCGA GGTGGACCTT 
GTCATGCACA GTCCCACCTT CAGAGGCGGT GGTGAGAATT CCCCTCAGAC CCCCAAGTTC 
CCTGTGTCAC CACAGACTCC TTACTTTAAC ATGTCTCGTG TCCCTCCAGG TCTGGCCAAG 
ACTCCTCTGT CAGCACTGGA ACTGAAGCCC CATGATCACG ATAACCACAC TTCCAGGGAC 
TCAAGAGTTC AACCCTTCAA CACCTCTTTC TCTTCCACCT ACCCTAGTCA CAGCAGTGAT 
GGAAGAAGAA CCGGTTCTTC AGACGTTCAC AGCCACACCC CCTCCTACGC AAACAGACCC 
ACCTCCGCTG AGGGCTCCCA GGTCAACGTC ATGGGAGTCC ACACAGTGCC CGGCAGTCCC 
AACACCCTCC ATCGCACCGT AGCCACCAAC ACTCCACCAA GCCCGGCCCT TCAGAGACGT 
CTGGGTCAAG CCAGTCCCTC ACTAGCCAGA CACCAACCCC CAGCTGGTGC CAACAGGGAC 
AGTGTCCCCT CCAGTCCTCT GATTGGCCGC AACCCTAAAA CAGCAGCTGC TGGCGTGCCC 
CCCAGCCCTC TAATGGGCCG CCGAACGGCG ATGAGCGGCC ACAGCACGCC TGATGACATC 
GGCCCACCAC CTTCTACGCC TGCCTTCCCT GTGTCCCCAC AGCTACCAGA AAAGAGGCAC 
ATGTCCACTG GAGACGTGGA GAGGACAGAT AACAAGAACC TTATGGCCGG TGTTGGCAGC 
AGTGCGCCAA ACCTGTCTGG CACGCACACG TTGACAGACG TCCAAAAGTC CATCTATGAT 
GGGTATCCGG ACATTAAGAT GAATGTGAAG TTTGTCCAAG ACACATCCAA GTTCTGGTAC 
AAGCCAGACA TCTCCAGAGA GCAAGCCATC AATCTGTTGA AGGACAGAGA GCCCGGGGCC 
TTCATCATAA GGGACAGCCA TTCCTTCCGT GGAGCTTACG GCCTTGCAAT GAAGGTGGCC 
TGCCCGCCGC CAACTGTCCA GCAGAACAAA AAAGTTGGAG ACTTGACCAA CGAGCTTGTG 
AGACACTTCC TGATTGAGAC AAGCTCCAGA GGTGTTCGAC TCAAAGGCTG TCCCAATGAA 
CCCTACTTTG GTTGCCTCTC AGCGCTGGTT TATCAACACT CAATGACCCC GCTAGCTCTG 
CCCTGTAAGC TGATGATCCC CACCAAAGAC CCGAATGAGG AAGCGCTGGA GTTGGCCACA 
CCCACTGACC CAGTTGTCGA GCTTCTGAAG CAAGGAGCAG TTCAGAAGGT TCCAGAGGAG 
GCTTACGCGT GTAACGTCCT CTACATCAAC TCGGTGGACA TGGAGTCTCT CACGGGGCCC 
CAGGCCATCG CCAAGGCGAT CAGCCTGACT TTGGCGACAA ACCCCCTGCC GGCCGCCACC 
ACCGTCCACT TCAAAGTGTC TATGCAGGGC ATCACGCTCA CCGACAGCCA GAGGAAACTT 
TTTTTCAGAC GCCACTATCC CATCAACACC ATAACGTTCT GTGACATTGA CCCGCAGAAC 
AGGAAATGGG GAAAAGAAGG AGGCGGTTCA GTGAAGCTGT TTGGTTTTGT GGCACGGAAA 
CAAGGAAGCA CAACCGACAA CGTGAGCCAC CTGTTCGCAG AGCTGGACCC AGATCAGCCG 
GCTTCCGCCA TCACCAGTTT TGTCTCCAAG ACGATGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840192.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696052.1 Takifugu rubripes tensin (LOC101064247), transcript variant X4, mRNA.

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CloneID	OTo71056
Clone ID Related Accession (Same CDS sequence)	XM_029840192.1
Accession Version	XM_029840192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGAGTACA ACGCTCGTAT CCGGGGCATC AGCGAAAGCA TGACACCGCA ACAATCTGAC 
CGTCACCGCT CCTATTCCTT CTCTGGCGTT CGCTCCAGAG GAATGACCCC AGAAGTGACA 
CAGGACAGCG GTCGACGTAG GACCACCAGT GAGGGACAGT ACCAGAGCAG CCACGACAGC 
ACACCAATGG TTGATTCACC GGACTTTGCC CACAGTTTGA CCCTCAACCC TGGAGGCAGA 
CCCAGAGAGG GCCAGATTCA CAGCTACAGG GAGGTGTTGG AGGACAGCGA GGTGGACCTT 
GTCATGCACA GTCCCACCTT CAGAGGCGGT GGTGAGAATT CCCCTCAGAC CCCCAAGTTC 
CCTGTGTCAC CACAGACTCC TTACTTTAAC ATGTCTCGTG TCCCTCCAGG TCTGGCCAAG 
ACTCCTCTGT CAGCACTGGA ACTGAAGCCC CATGATCACG ACTCTGATTC AAATGAGGGA 
GAGCAAGATA ACCACACTTC CAGGGACTCA AGAGTTCAAC CCTTCAACAC CTCTTTCTCT 
TCCACCTACC CTAGTCACAG CAGTGATGGA AGAAGAACCG GTTCTTCAGA CGTTCACAGC 
CACACCCCCT CCTACGCAAA CAGACCCACC TCCGCTGAGG GCTCCCAGGT CAACGTCATG 
GGAGTCCACA CAGTGCCCGG CAGTCCCAAC ACCCTCCATC GCACCGTAGC CACCAACACT 
CCACCAAGCC CGGCCCTTCA GAGACGTCTG GGTCAAGCCA GTCCCTCACT AGCCAGACAC 
CAACCCCCAG CTGGTGCCAA CAGGGACAGT GTCCCCTCCA GTCCTCTGAT TGGCCGCAAC 
CCTAAAACAG CAGCTGCTGG CGTGCCCCCC AGCCCTCTAA TGGGCCGCCG AACGGCGATG 
AGCGGCCACA GCACGCCTGA TGACATCGGC CCACCACCTT CTACGCCTGC CTTCCCTGTG 
TCCCCACAGC TACCAGAAAA GAGGCACATG TCCACTGGAG ACGTGGAGAG GACAGATAAC 
AAGAACCTTA TGGCCGGTGT TGGCAGCAGT GCGCCAAACC TGTCTGGCAC GCACACGTTG 
ACAGACGTCC AAAAGTCCAT CTATGATGGG TATCCGGACA TTAAGATGAA TGTGAAGTTT 
GTCCAAGACA CATCCAAGTT CTGGTACAAG CCAGACATCT CCAGAGAGCA AGCCATCAAT 
CTGTTGAAGG ACAGAGAGCC CGGGGCCTTC ATCATAAGGG ACAGCCATTC CTTCCGTGGA 
GCTTACGGCC TTGCAATGAA GGTGGCCTGC CCGCCGCCAA CTGTCCAGCA GAACAAAAAA 
GTTGGAGACT TGACCAACGA GCTTGTGAGA CACTTCCTGA TTGAGACAAG CTCCAGAGGT 
GTTCGACTCA AAGGCTGTCC CAATGAACCC TACTTTGGTT GCCTCTCAGC GCTGGTTTAT 
CAACACTCAA TGACCCCGCT AGCTCTGCCC TGTAAGCTGA TGATCCCCAC CAAAGACCCG 
AATGAGGAAG CGCTGGAGTT GGCCACACCC ACTGACCCAG TTGTCGAGCT TCTGAAGCAA 
GGAGCAGCGT GTAACGTCCT CTACATCAAC TCGGTGGACA TGGAGTCTCT CACGGGGCCC 
CAGGCCATCG CCAAGGCGAT CAGCCTGACT TTGGCGACAA ACCCCCTGCC GGCCGCCACC 
ACCGTCCACT TCAAAGTGTC TATGCAGGGC ATCACGCTCA CCGACAGCCA GAGGAAACTT 
TTTTTCAGAC GCCACTATCC CATCAACACC ATAACGTTCT GTGACATTGA CCCGCAGAAC 
AGGAAATGGG GAAAAGAAGG AGGCGGTTCA GTGAAGCTGT TTGGTTTTGT GGCACGGAAA 
CAAGGAAGCA CAACCGACAA CGTGAGCCAC CTGTTCGCAG AGCTGGACCC AGATCAGCCG 
GCTTCCGCCA TCACCAGTTT TGTCTCCAAG ACGATGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696052.1
CDS	647..5731
Translation	MARLNWCLAAVISWGKFLFACFFPLRGAKRDTPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELPPTPQLPLKHVDTPGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840192.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840192.1

ORF Insert Sequence:

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGAGTACA ACGCTCGTAT CCGGGGCATC AGCGAAAGCA TGACACCGCA ACAATCTGAC 
CGTCACCGCT CCTATTCCTT CTCTGGCGTT CGCTCCAGAG GAATGACCCC AGAAGTGACA 
CAGGACAGCG GTCGACGTAG GACCACCAGT GAGGGACAGT ACCAGAGCAG CCACGACAGC 
ACACCAATGG TTGATTCACC GGACTTTGCC CACAGTTTGA CCCTCAACCC TGGAGGCAGA 
CCCAGAGAGG GCCAGATTCA CAGCTACAGG GAGGTGTTGG AGGACAGCGA GGTGGACCTT 
GTCATGCACA GTCCCACCTT CAGAGGCGGT GGTGAGAATT CCCCTCAGAC CCCCAAGTTC 
CCTGTGTCAC CACAGACTCC TTACTTTAAC ATGTCTCGTG TCCCTCCAGG TCTGGCCAAG 
ACTCCTCTGT CAGCACTGGA ACTGAAGCCC CATGATCACG ACTCTGATTC AAATGAGGGA 
GAGCAAGATA ACCACACTTC CAGGGACTCA AGAGTTCAAC CCTTCAACAC CTCTTTCTCT 
TCCACCTACC CTAGTCACAG CAGTGATGGA AGAAGAACCG GTTCTTCAGA CGTTCACAGC 
CACACCCCCT CCTACGCAAA CAGACCCACC TCCGCTGAGG GCTCCCAGGT CAACGTCATG 
GGAGTCCACA CAGTGCCCGG CAGTCCCAAC ACCCTCCATC GCACCGTAGC CACCAACACT 
CCACCAAGCC CGGCCCTTCA GAGACGTCTG GGTCAAGCCA GTCCCTCACT AGCCAGACAC 
CAACCCCCAG CTGGTGCCAA CAGGGACAGT GTCCCCTCCA GTCCTCTGAT TGGCCGCAAC 
CCTAAAACAG CAGCTGCTGG CGTGCCCCCC AGCCCTCTAA TGGGCCGCCG AACGGCGATG 
AGCGGCCACA GCACGCCTGA TGACATCGGC CCACCACCTT CTACGCCTGC CTTCCCTGTG 
TCCCCACAGC TACCAGAAAA GAGGCACATG TCCACTGGAG ACGTGGAGAG GACAGATAAC 
AAGAACCTTA TGGCCGGTGT TGGCAGCAGT GCGCCAAACC TGTCTGGCAC GCACACGTTG 
ACAGACGTCC AAAAGTCCAT CTATGATGGG TATCCGGACA TTAAGATGAA TGTGAAGTTT 
GTCCAAGACA CATCCAAGTT CTGGTACAAG CCAGACATCT CCAGAGAGCA AGCCATCAAT 
CTGTTGAAGG ACAGAGAGCC CGGGGCCTTC ATCATAAGGG ACAGCCATTC CTTCCGTGGA 
GCTTACGGCC TTGCAATGAA GGTGGCCTGC CCGCCGCCAA CTGTCCAGCA GAACAAAAAA 
GTTGGAGACT TGACCAACGA GCTTGTGAGA CACTTCCTGA TTGAGACAAG CTCCAGAGGT 
GTTCGACTCA AAGGCTGTCC CAATGAACCC TACTTTGGTT GCCTCTCAGC GCTGGTTTAT 
CAACACTCAA TGACCCCGCT AGCTCTGCCC TGTAAGCTGA TGATCCCCAC CAAAGACCCG 
AATGAGGAAG CGCTGGAGTT GGCCACACCC ACTGACCCAG TTGTCGAGCT TCTGAAGCAA 
GGAGCAGCGT GTAACGTCCT CTACATCAAC TCGGTGGACA TGGAGTCTCT CACGGGGCCC 
CAGGCCATCG CCAAGGCGAT CAGCCTGACT TTGGCGACAA ACCCCCTGCC GGCCGCCACC 
ACCGTCCACT TCAAAGTGTC TATGCAGGGC ATCACGCTCA CCGACAGCCA GAGGAAACTT 
TTTTTCAGAC GCCACTATCC CATCAACACC ATAACGTTCT GTGACATTGA CCCGCAGAAC 
AGGAAATGGG GAAAAGAAGG AGGCGGTTCA GTGAAGCTGT TTGGTTTTGT GGCACGGAAA 
CAAGGAAGCA CAACCGACAA CGTGAGCCAC CTGTTCGCAG AGCTGGACCC AGATCAGCCG 
GCTTCCGCCA TCACCAGTTT TGTCTCCAAG ACGATGAAGC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840193.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696053.1 Takifugu rubripes tensin (LOC101064247), transcript variant X5, mRNA.

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CloneID	OTo71057
Clone ID Related Accession (Same CDS sequence)	XM_029840193.1
Accession Version	XM_029840193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGGG ATCTGCGAGG 
TCCTGCAAGA GTGTGGAGAT AAGGCGGAAG CAGTCTCGAA GCCAGAGTGT GGTTCTGACC 
ATGGAGGAGA ACTATGAGGT AGACCTGGTC TACATCACGG AGAGGATCAT CTCCCTCTGC 
TTCCCCATTG GCATGGAGGA CCAGGGCTAC ATCGCCAACC TCAAGGAGGT GGCCACCATG 
CTGCGATCCA AACATGGAGA TCATTATTTG GTGCTGAACC TGAGTGAATG GAGGACTGAG 
TTATCGAAGC TAAACCACAA GGTCTTGGAA TTTGGCTGGC CGGACTTTCA CGCACCAGCC 
CTGGACAAGA TCTGTAGCAT GTGCAAAGCG ATTGACACGT GGCTCAATGG AGACTCGCAC 
AATGTGGTGG TGCTTCACAA CAAGGGGAAC CGAGGTCGAA CTGGGGTCGT GGTGGCTGCT 
TACATGCACT ATAGCAACAT ATCAGCAAGT GCTGACCAGG CCTTGGACAG GTTCGCCATG 
AGACGGTTCT ACGAGGATAA AGCACTTCCT GTGGGTCAGC CGTCACAAAA AAGGTATGTG 
CGATACTTCA ATGGCCTTCT GTCGGGGCAC ATCAAAATCA ACAACAAACC TCTCTTCCTG 
CACCACGTCA TCATGCACGG TATCCCCAAC TTTGAGTCTA AAGGAGGCTG CCGCCCTTTC 
CTGAAGATCT ACCAAGCGAT GCAGCCAGTC TACACGTCAG GGATATACAA CGTGCAGGGA 
GACAGTAACA CTAGTATCTG CATCACCATC GAACCAGGCC TCCTCCTGAA GGGAGACATC 
CTGCTGAAGT GTTACCACAA ACGATACAGA GGCCCGACCA GAGACGTAGT GTTCCGGGTG 
CAGTTCCACA CCTGTGCCAT CCACGACCTT GGCGTGGTGT TCGGGAAGAG CGAACTGGAC 
GAGACCTTCA AAGACGACAG GTTTCCTGAA TACGGGAAAG TGGAGTTTGT TTTCTCCTAT 
GCACCAGAAA AAATTAGAGG CTTAGACCAC CTGGAGAACG GCCCCAGTGT TTCCGTGGAC 
TACAACACCC AGGAAAACCT GATTCGCTGT GATTCATATG ACAACTTTAG CCAGCGCTCT 
GAAGATGCCG TGGATGGAGA GCGTGAGGTC ATTCACACTC AAGGTCCACT TGATGGAAGC 
CTTTATGCCC GGGTCTGTAA GAAAGACTCC CTGGAGGGAG TTGTCGCCAT CAACGGTAAT 
CCAGTCCATG AAAATTCCTT AGCCAGCACA GAGAACCAGT TACAACACAC AAGCTCCTTG 
CTTCAAACCG CCGAACAGAC CCTTCCTGTG ATTGGCCACG CTTCTCTTCC TGCTGTTGAC 
CACACACTCT CTGTGAGCAG TGACTCGGGC AACTCCACTG CATCCATTAA AACTGATCGT 
ACCGACGAGC ACAGCCAGCC AGTGCACAGC ACTGTCAACC ACAACAATCC GGCAGTTCCC 
CACCCGACAC TTAGTCCCCA GGAGAAAAAA GAGCTTGATC AGCTCCTTAG TGGCCTCGAA 
GCACCGACTC TTCAACGACA AGCCTACCTG GCTACATCCA CCAGTCCGGG AGGCGGTGTC 
CGACACCTGG TCCCAGCACA AGTGCACGTT AACGGAGGCC ACCCAAGGCT AGTTCCTGTC 
CCCTCAACGG AAGAGCGTGA GACGGACATT CTTGACGACG AGCTGCCAAA TAGCCAAGAG 
GGAAACAGTG TGGACAGCCT GGGTACACTG TCATCCTTAG AGGGTCAAGC AACCCCAGGG 
GATCTCTATT ACCAACAAGA GAAGTCCGTC AGCAGACAAA ACGACGGGCC ATATCTTGAG 
AGAGGCGTTT GTCGGGAAAA AAAAAACGAG ATGCCCATGC ATGGAGTTCA TACTCCGATG 
ATGCAGGAAA GACCTGTGGA CTCTGTATCC CCACAAGGGG GATATAACAA ATACCAGAAC 
GGTGGTGGGA TGTACCACTC TGAATCCTAT GGGATTCCAC CTGCCAGCAG CCCGGAGGGA 
AACCCTAAAC TGATGCCCAG GGCCCCAGAG AGAAGCACAA GTAGCCGGGA GGCTGTTCAA 
AGGGGCCTCA ATGCCTGGCA CCAGTATAGC CTCCCAGATG ATCCATTTGG ACCTCCCCTG 
CAGTCAACCC ATAGCATGCC ACAGTTCCCC ACAGCAGCCT CACAGCGGGA CATAGAGCAG 
TCCATCGAGG CACTGAATAT GCTCATGCTT GACCTGGACC CCATTAACTC TCACATGTCC 
AAGTCCCATA GTGCACCCTC CGGGGAGAAC ATCCTGAATT CATCCCAGGC ACCCTTCTCC 
CAGACCCTCT CCAGACCCTC ATACCAAGGA GACTCCGCCA TCCACAGATA CAATAACTCT 
GAATCTGTCA ACAATCCCTC CCATCAGCCC ATATGGTCAG CTGGAAGAGT GTCAGCAGTG 
CCTAACCAGA GCCCTATTAT GGATTCTCAA GCACATTTGC CCCAGAGATC CAGCTCGAGC 
TGTCAACACC AGAAAACCAC CCCAACACAC ACCCCAGAGC CCAATCTCCA TGCACGACAA 
GGAGCCATCC ATTACCCCAT TGATCCCTCC TTTAATTTCA ACCAGCATCT CCCAGTTAGG 
CCTGCAAACA CTTTCCCAGG TGTTTCCCCC TCCCCAGACC TGCAGGGTTC GTCTCCATAC 
CAGGGCTTCA GTGCCTCTTC CTCACCCCTT CCTGCGCTCA CTCCACAGCC AAAGAACACA 
TCGTCTTCAT CCCTGCCTCA AGAACAAGAT GTTGAGGAAG AGGCGCTGAA CTTGGAAGGT 
CTGGTGGCTC ATCGCATTGC CGAGTACAAC GCTCGTATCC GGGGCATCAG CGAAAGCATG 
ACACCGCAAC AATCTGACCG TCACCGCTCC TATTCCTTCT CTGGCGTTCG CTCCAGAGGA 
ATGACCCCAG AAGTGACACA GGACAGCGGT CGACGTAGGA CCACCAGTGA GGGACAGTAC 
CAGAGCAGCC ACGACAGCAC ACCAATGGTT GATTCACCGG ACTTTGCCCA CAGTTTGACC 
CTCAACCCTG GAGGCAGACC CAGAGAGGGC CAGATTCACA GCTACAGGGA GGTGTTGGAG 
GACAGCGAGG TGGACCTTGT CATGCACAGT CCCACCTTCA GAGGCGGTGG TGAGAATTCC 
CCTCAGACCC CCAAGTTCCC TGTGTCACCA CAGACTCCTT ACTTTAACAT GTCTCGTGTC 
CCTCCAGGTC TGGCCAAGAC TCCTCTGTCA GCACTGGAAC TGAAGCCCCA TGATCACGAC 
TCTGATTCAA ATGAGGGAGA GCAAGATAAC CACACTTCCA GGGACTCAAG AGTTCAACCC 
TTCAACACCT CTTTCTCTTC CACCTACCCT AGTCACAGCA GTGATGGAAG AAGAACCGGT 
TCTTCAGACG TTCACAGCCA CACCCCCTCC TACGCAAACA GACCCACCTC CGCTGAGGGC 
TCCCAGGTCA ACGTCATGGG AGTCCACACA GTGCCCGGCA GTCCCAACAC CCTCCATCGC 
ACCGTAGCCA CCAACACTCC ACCAAGCCCG GCCCTTCAGA GACGTCTGGG TCAAGCCAGT 
CCCTCACTAG CCAGACACCA ACCCCCAGCT GGTGCCAACA GGGACAGTGT CCCCTCCAGT 
CCTCTGATTG GCCGCAACCC TAAAACAGCA GCTGCTGGCG TGCCCCCCAG CCCTCTAATG 
GGCCGCCGAA CGGCGATGAG CGGCCACAGC ACGCCTGATG ACATCGGCCC ACCACCTTCT 
ACGCCTGCCT TCCCTGTGTC CCCACAGCTA CCAGAAAAGA GGCACATGTC CACTGGAGAC 
GTGGAGAGGA CAGATAACAA GAACCTTATG GCCGGTGTTG GCAGCAGTGC GCCAAACCTG 
TCTGGCACGC ACACGTTGAC AGACGTCCAA AAGTCCATCT ATGATGGGTA TCCGGACATT 
AAGATGAATG TGAAGTTTGT CCAAGACACA TCCAAGTTCT GGTACAAGCC AGACATCTCC 
AGAGAGCAAG CCATCAATCT GTTGAAGGAC AGAGAGCCCG GGGCCTTCAT CATAAGGGAC 
AGCCATTCCT TCCGTGGAGC TTACGGCCTT GCAATGAAGG TGGCCTGCCC GCCGCCAACT 
GTCCAGCAGA ACAAAAAAGT TGGAGACTTG ACCAACGAGC TTGTGAGACA CTTCCTGATT 
GAGACAAGCT CCAGAGGTGT TCGACTCAAA GGCTGTCCCA ATGAACCCTA CTTTGGTTGC 
CTCTCAGCGC TGGTTTATCA ACACTCAATG ACCCCGCTAG CTCTGCCCTG TAAGCTGATG 
ATCCCCACCA AAGACCCGAA TGAGGAAGCG CTGGAGTTGG CCACACCCAC TGACCCAGTT 
GTCGAGCTTC TGAAGCAAGG AGCAGTTCAG AAGGTTCCAG AGGAGGCTTA CGCGTGTAAC 
GTCCTCTACA TCAACTCGGT GGACATGGAG TCTCTCACGG GGCCCCAGGC CATCGCCAAG 
GCGATCAGCC TGACTTTGGC GACAAACCCC CTGCCGGCCG CCACCACCGT CCACTTCAAA 
GTGTCTATGC AGGGCATCAC GCTCACCGAC AGCCAGAGGA AACTTTTTTT CAGACGCCAC 
TATCCCATCA ACACCATAAC GTTCTGTGAC ATTGACCCGC AGAACAGGAA ATGGGGAAAA 
GAAGGAGGCG GTTCAGTGAA GCTGTTTGGT TTTGTGGCAC GGAAACAAGG AAGCACAACC 
GACAACGTGA GCCACCTGTT CGCAGAGCTG GACCCAGATC AGCCGGCTTC CGCCATCACC 
AGTTTTGTCT CCAAGACGAT GAAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696053.1
CDS	646..5715
Translation	MARLNWCLAAVISWGKFLFACFFPLRGAKRDTPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840193.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840193.1

ORF Insert Sequence:

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGGG ATCTGCGAGG 
TCCTGCAAGA GTGTGGAGAT AAGGCGGAAG CAGTCTCGAA GCCAGAGTGT GGTTCTGACC 
ATGGAGGAGA ACTATGAGGT AGACCTGGTC TACATCACGG AGAGGATCAT CTCCCTCTGC 
TTCCCCATTG GCATGGAGGA CCAGGGCTAC ATCGCCAACC TCAAGGAGGT GGCCACCATG 
CTGCGATCCA AACATGGAGA TCATTATTTG GTGCTGAACC TGAGTGAATG GAGGACTGAG 
TTATCGAAGC TAAACCACAA GGTCTTGGAA TTTGGCTGGC CGGACTTTCA CGCACCAGCC 
CTGGACAAGA TCTGTAGCAT GTGCAAAGCG ATTGACACGT GGCTCAATGG AGACTCGCAC 
AATGTGGTGG TGCTTCACAA CAAGGGGAAC CGAGGTCGAA CTGGGGTCGT GGTGGCTGCT 
TACATGCACT ATAGCAACAT ATCAGCAAGT GCTGACCAGG CCTTGGACAG GTTCGCCATG 
AGACGGTTCT ACGAGGATAA AGCACTTCCT GTGGGTCAGC CGTCACAAAA AAGGTATGTG 
CGATACTTCA ATGGCCTTCT GTCGGGGCAC ATCAAAATCA ACAACAAACC TCTCTTCCTG 
CACCACGTCA TCATGCACGG TATCCCCAAC TTTGAGTCTA AAGGAGGCTG CCGCCCTTTC 
CTGAAGATCT ACCAAGCGAT GCAGCCAGTC TACACGTCAG GGATATACAA CGTGCAGGGA 
GACAGTAACA CTAGTATCTG CATCACCATC GAACCAGGCC TCCTCCTGAA GGGAGACATC 
CTGCTGAAGT GTTACCACAA ACGATACAGA GGCCCGACCA GAGACGTAGT GTTCCGGGTG 
CAGTTCCACA CCTGTGCCAT CCACGACCTT GGCGTGGTGT TCGGGAAGAG CGAACTGGAC 
GAGACCTTCA AAGACGACAG GTTTCCTGAA TACGGGAAAG TGGAGTTTGT TTTCTCCTAT 
GCACCAGAAA AAATTAGAGG CTTAGACCAC CTGGAGAACG GCCCCAGTGT TTCCGTGGAC 
TACAACACCC AGGAAAACCT GATTCGCTGT GATTCATATG ACAACTTTAG CCAGCGCTCT 
GAAGATGCCG TGGATGGAGA GCGTGAGGTC ATTCACACTC AAGGTCCACT TGATGGAAGC 
CTTTATGCCC GGGTCTGTAA GAAAGACTCC CTGGAGGGAG TTGTCGCCAT CAACGGTAAT 
CCAGTCCATG AAAATTCCTT AGCCAGCACA GAGAACCAGT TACAACACAC AAGCTCCTTG 
CTTCAAACCG CCGAACAGAC CCTTCCTGTG ATTGGCCACG CTTCTCTTCC TGCTGTTGAC 
CACACACTCT CTGTGAGCAG TGACTCGGGC AACTCCACTG CATCCATTAA AACTGATCGT 
ACCGACGAGC ACAGCCAGCC AGTGCACAGC ACTGTCAACC ACAACAATCC GGCAGTTCCC 
CACCCGACAC TTAGTCCCCA GGAGAAAAAA GAGCTTGATC AGCTCCTTAG TGGCCTCGAA 
GCACCGACTC TTCAACGACA AGCCTACCTG GCTACATCCA CCAGTCCGGG AGGCGGTGTC 
CGACACCTGG TCCCAGCACA AGTGCACGTT AACGGAGGCC ACCCAAGGCT AGTTCCTGTC 
CCCTCAACGG AAGAGCGTGA GACGGACATT CTTGACGACG AGCTGCCAAA TAGCCAAGAG 
GGAAACAGTG TGGACAGCCT GGGTACACTG TCATCCTTAG AGGGTCAAGC AACCCCAGGG 
GATCTCTATT ACCAACAAGA GAAGTCCGTC AGCAGACAAA ACGACGGGCC ATATCTTGAG 
AGAGGCGTTT GTCGGGAAAA AAAAAACGAG ATGCCCATGC ATGGAGTTCA TACTCCGATG 
ATGCAGGAAA GACCTGTGGA CTCTGTATCC CCACAAGGGG GATATAACAA ATACCAGAAC 
GGTGGTGGGA TGTACCACTC TGAATCCTAT GGGATTCCAC CTGCCAGCAG CCCGGAGGGA 
AACCCTAAAC TGATGCCCAG GGCCCCAGAG AGAAGCACAA GTAGCCGGGA GGCTGTTCAA 
AGGGGCCTCA ATGCCTGGCA CCAGTATAGC CTCCCAGATG ATCCATTTGG ACCTCCCCTG 
CAGTCAACCC ATAGCATGCC ACAGTTCCCC ACAGCAGCCT CACAGCGGGA CATAGAGCAG 
TCCATCGAGG CACTGAATAT GCTCATGCTT GACCTGGACC CCATTAACTC TCACATGTCC 
AAGTCCCATA GTGCACCCTC CGGGGAGAAC ATCCTGAATT CATCCCAGGC ACCCTTCTCC 
CAGACCCTCT CCAGACCCTC ATACCAAGGA GACTCCGCCA TCCACAGATA CAATAACTCT 
GAATCTGTCA ACAATCCCTC CCATCAGCCC ATATGGTCAG CTGGAAGAGT GTCAGCAGTG 
CCTAACCAGA GCCCTATTAT GGATTCTCAA GCACATTTGC CCCAGAGATC CAGCTCGAGC 
TGTCAACACC AGAAAACCAC CCCAACACAC ACCCCAGAGC CCAATCTCCA TGCACGACAA 
GGAGCCATCC ATTACCCCAT TGATCCCTCC TTTAATTTCA ACCAGCATCT CCCAGTTAGG 
CCTGCAAACA CTTTCCCAGG TGTTTCCCCC TCCCCAGACC TGCAGGGTTC GTCTCCATAC 
CAGGGCTTCA GTGCCTCTTC CTCACCCCTT CCTGCGCTCA CTCCACAGCC AAAGAACACA 
TCGTCTTCAT CCCTGCCTCA AGAACAAGAT GTTGAGGAAG AGGCGCTGAA CTTGGAAGGT 
CTGGTGGCTC ATCGCATTGC CGAGTACAAC GCTCGTATCC GGGGCATCAG CGAAAGCATG 
ACACCGCAAC AATCTGACCG TCACCGCTCC TATTCCTTCT CTGGCGTTCG CTCCAGAGGA 
ATGACCCCAG AAGTGACACA GGACAGCGGT CGACGTAGGA CCACCAGTGA GGGACAGTAC 
CAGAGCAGCC ACGACAGCAC ACCAATGGTT GATTCACCGG ACTTTGCCCA CAGTTTGACC 
CTCAACCCTG GAGGCAGACC CAGAGAGGGC CAGATTCACA GCTACAGGGA GGTGTTGGAG 
GACAGCGAGG TGGACCTTGT CATGCACAGT CCCACCTTCA GAGGCGGTGG TGAGAATTCC 
CCTCAGACCC CCAAGTTCCC TGTGTCACCA CAGACTCCTT ACTTTAACAT GTCTCGTGTC 
CCTCCAGGTC TGGCCAAGAC TCCTCTGTCA GCACTGGAAC TGAAGCCCCA TGATCACGAC 
TCTGATTCAA ATGAGGGAGA GCAAGATAAC CACACTTCCA GGGACTCAAG AGTTCAACCC 
TTCAACACCT CTTTCTCTTC CACCTACCCT AGTCACAGCA GTGATGGAAG AAGAACCGGT 
TCTTCAGACG TTCACAGCCA CACCCCCTCC TACGCAAACA GACCCACCTC CGCTGAGGGC 
TCCCAGGTCA ACGTCATGGG AGTCCACACA GTGCCCGGCA GTCCCAACAC CCTCCATCGC 
ACCGTAGCCA CCAACACTCC ACCAAGCCCG GCCCTTCAGA GACGTCTGGG TCAAGCCAGT 
CCCTCACTAG CCAGACACCA ACCCCCAGCT GGTGCCAACA GGGACAGTGT CCCCTCCAGT 
CCTCTGATTG GCCGCAACCC TAAAACAGCA GCTGCTGGCG TGCCCCCCAG CCCTCTAATG 
GGCCGCCGAA CGGCGATGAG CGGCCACAGC ACGCCTGATG ACATCGGCCC ACCACCTTCT 
ACGCCTGCCT TCCCTGTGTC CCCACAGCTA CCAGAAAAGA GGCACATGTC CACTGGAGAC 
GTGGAGAGGA CAGATAACAA GAACCTTATG GCCGGTGTTG GCAGCAGTGC GCCAAACCTG 
TCTGGCACGC ACACGTTGAC AGACGTCCAA AAGTCCATCT ATGATGGGTA TCCGGACATT 
AAGATGAATG TGAAGTTTGT CCAAGACACA TCCAAGTTCT GGTACAAGCC AGACATCTCC 
AGAGAGCAAG CCATCAATCT GTTGAAGGAC AGAGAGCCCG GGGCCTTCAT CATAAGGGAC 
AGCCATTCCT TCCGTGGAGC TTACGGCCTT GCAATGAAGG TGGCCTGCCC GCCGCCAACT 
GTCCAGCAGA ACAAAAAAGT TGGAGACTTG ACCAACGAGC TTGTGAGACA CTTCCTGATT 
GAGACAAGCT CCAGAGGTGT TCGACTCAAA GGCTGTCCCA ATGAACCCTA CTTTGGTTGC 
CTCTCAGCGC TGGTTTATCA ACACTCAATG ACCCCGCTAG CTCTGCCCTG TAAGCTGATG 
ATCCCCACCA AAGACCCGAA TGAGGAAGCG CTGGAGTTGG CCACACCCAC TGACCCAGTT 
GTCGAGCTTC TGAAGCAAGG AGCAGTTCAG AAGGTTCCAG AGGAGGCTTA CGCGTGTAAC 
GTCCTCTACA TCAACTCGGT GGACATGGAG TCTCTCACGG GGCCCCAGGC CATCGCCAAG 
GCGATCAGCC TGACTTTGGC GACAAACCCC CTGCCGGCCG CCACCACCGT CCACTTCAAA 
GTGTCTATGC AGGGCATCAC GCTCACCGAC AGCCAGAGGA AACTTTTTTT CAGACGCCAC 
TATCCCATCA ACACCATAAC GTTCTGTGAC ATTGACCCGC AGAACAGGAA ATGGGGAAAA 
GAAGGAGGCG GTTCAGTGAA GCTGTTTGGT TTTGTGGCAC GGAAACAAGG AAGCACAACC 
GACAACGTGA GCCACCTGTT CGCAGAGCTG GACCCAGATC AGCCGGCTTC CGCCATCACC 
AGTTTTGTCT CCAAGACGAT GAAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840194.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696054.1 Takifugu rubripes tensin (LOC101064247), transcript variant X6, mRNA.

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CloneID	OTo71058
Clone ID Related Accession (Same CDS sequence)	XM_029840194.1
Accession Version	XM_029840194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGAGTACA ACGCTCGTAT CCGGGGCATC AGCGAAAGCA TGACACCGCA ACAATCTGAC 
CGTCACCGCT CCTATTCCTT CTCTGGCGTT CGCTCCAGAG GAATGACCCC AGAAGTGACA 
CAGGACAGCG GTCGACGTAG GACCACCAGT GAGGGACAGT ACCAGAGCAG CCACGACAGC 
ACACCAATGG TTGATTCACC GGACTTTGCC CACAGTTTGA CCCTCAACCC TGGAGGCAGA 
CCCAGAGAGG GCCAGATTCA CAGCTACAGG GAGGTGTTGG AGGACAGCGA GGTGGACCTT 
GTCATGCACA GTCCCACCTT CAGAGGCGGT GCTCGTGTCC CTCCAGGTCT GGCCAAGACT 
CCTCTGTCAG CACTGGAACT GAAGCCCCAT GATCACGACT CTGATTCAAA TGAGGGAGAG 
CAAGATAACC ACACTTCCAG GGACTCAAGA GTTCAACCCT TCAACACCTC TTTCTCTTCC 
ACCTACCCTA GTCACAGCAG TGATGGAAGA AGAACCGGTT CTTCAGACGT TCACAGCCAC 
ACCCCCTCCT ACGCAAACAG ACCCACCTCC GCTGAGGGCT CCCAGGTCAA CGTCATGGGA 
GTCCACACAG TGCCCGGCAG TCCCAACACC CTCCATCGCA CCGTAGCCAC CAACACTCCA 
CCAAGCCCGG CCCTTCAGAG ACGTCTGGGT CAAGCCAGTC CCTCACTAGC CAGACACCAA 
CCCCCAGCTG GTGCCAACAG GGACAGTGTC CCCTCCAGTC CTCTGATTGG CCGCAACCCT 
AAAACAGCAG CTGCTGGCGT GCCCCCCAGC CCTCTAATGG GCCGCCGAAC GGCGATGAGC 
GGCCACAGCA CGCCTGATGA CATCGGCCCA CCACCTTCTA CGCCTGCCTT CCCTGTGTCC 
CCACAGCTAC CAGAAAAGAG GCACATGTCC ACTGGAGACG TGGAGAGGAC AGATAACAAG 
AACCTTATGG CCGGTGTTGG CAGCAGTGCG CCAAACCTGT CTGGCACGCA CACGTTGACA 
GACGTCCAAA AGTCCATCTA TGATGGGTAT CCGGACATTA AGATGAATGT GAAGTTTGTC 
CAAGACACAT CCAAGTTCTG GTACAAGCCA GACATCTCCA GAGAGCAAGC CATCAATCTG 
TTGAAGGACA GAGAGCCCGG GGCCTTCATC ATAAGGGACA GCCATTCCTT CCGTGGAGCT 
TACGGCCTTG CAATGAAGGT GGCCTGCCCG CCGCCAACTG TCCAGCAGAA CAAAAAAGTT 
GGAGACTTGA CCAACGAGCT TGTGAGACAC TTCCTGATTG AGACAAGCTC CAGAGGTGTT 
CGACTCAAAG GCTGTCCCAA TGAACCCTAC TTTGGTTGCC TCTCAGCGCT GGTTTATCAA 
CACTCAATGA CCCCGCTAGC TCTGCCCTGT AAGCTGATGA TCCCCACCAA AGACCCGAAT 
GAGGAAGCGC TGGAGTTGGC CACACCCACT GACCCAGTTG TCGAGCTTCT GAAGCAAGGA 
GCAGTTCAGA AGGTTCCAGA GGAGGCTTAC GCGTGTAACG TCCTCTACAT CAACTCGGTG 
GACATGGAGT CTCTCACGGG GCCCCAGGCC ATCGCCAAGG CGATCAGCCT GACTTTGGCG 
ACAAACCCCC TGCCGGCCGC CACCACCGTC CACTTCAAAG TGTCTATGCA GGGCATCACG 
CTCACCGACA GCCAGAGGAA ACTTTTTTTC AGACGCCACT ATCCCATCAA CACCATAACG 
TTCTGTGACA TTGACCCGCA GAACAGGAAA TGGGGAAAAG AAGGAGGCGG TTCAGTGAAG 
CTGTTTGGTT TTGTGGCACG GAAACAAGGA AGCACAACCG ACAACGTGAG CCACCTGTTC 
GCAGAGCTGG ACCCAGATCA GCCGGCTTCC GCCATCACCA GTTTTGTCTC CAAGACGATG 
AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696054.1
CDS	647..5695
Translation	MARLNWCLAAVISWGKFLFACFFPLRGAKRDTPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELPPTPQLPLKHVDTPGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGARVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840194.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840194.1

ORF Insert Sequence:

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGAGTACA ACGCTCGTAT CCGGGGCATC AGCGAAAGCA TGACACCGCA ACAATCTGAC 
CGTCACCGCT CCTATTCCTT CTCTGGCGTT CGCTCCAGAG GAATGACCCC AGAAGTGACA 
CAGGACAGCG GTCGACGTAG GACCACCAGT GAGGGACAGT ACCAGAGCAG CCACGACAGC 
ACACCAATGG TTGATTCACC GGACTTTGCC CACAGTTTGA CCCTCAACCC TGGAGGCAGA 
CCCAGAGAGG GCCAGATTCA CAGCTACAGG GAGGTGTTGG AGGACAGCGA GGTGGACCTT 
GTCATGCACA GTCCCACCTT CAGAGGCGGT GCTCGTGTCC CTCCAGGTCT GGCCAAGACT 
CCTCTGTCAG CACTGGAACT GAAGCCCCAT GATCACGACT CTGATTCAAA TGAGGGAGAG 
CAAGATAACC ACACTTCCAG GGACTCAAGA GTTCAACCCT TCAACACCTC TTTCTCTTCC 
ACCTACCCTA GTCACAGCAG TGATGGAAGA AGAACCGGTT CTTCAGACGT TCACAGCCAC 
ACCCCCTCCT ACGCAAACAG ACCCACCTCC GCTGAGGGCT CCCAGGTCAA CGTCATGGGA 
GTCCACACAG TGCCCGGCAG TCCCAACACC CTCCATCGCA CCGTAGCCAC CAACACTCCA 
CCAAGCCCGG CCCTTCAGAG ACGTCTGGGT CAAGCCAGTC CCTCACTAGC CAGACACCAA 
CCCCCAGCTG GTGCCAACAG GGACAGTGTC CCCTCCAGTC CTCTGATTGG CCGCAACCCT 
AAAACAGCAG CTGCTGGCGT GCCCCCCAGC CCTCTAATGG GCCGCCGAAC GGCGATGAGC 
GGCCACAGCA CGCCTGATGA CATCGGCCCA CCACCTTCTA CGCCTGCCTT CCCTGTGTCC 
CCACAGCTAC CAGAAAAGAG GCACATGTCC ACTGGAGACG TGGAGAGGAC AGATAACAAG 
AACCTTATGG CCGGTGTTGG CAGCAGTGCG CCAAACCTGT CTGGCACGCA CACGTTGACA 
GACGTCCAAA AGTCCATCTA TGATGGGTAT CCGGACATTA AGATGAATGT GAAGTTTGTC 
CAAGACACAT CCAAGTTCTG GTACAAGCCA GACATCTCCA GAGAGCAAGC CATCAATCTG 
TTGAAGGACA GAGAGCCCGG GGCCTTCATC ATAAGGGACA GCCATTCCTT CCGTGGAGCT 
TACGGCCTTG CAATGAAGGT GGCCTGCCCG CCGCCAACTG TCCAGCAGAA CAAAAAAGTT 
GGAGACTTGA CCAACGAGCT TGTGAGACAC TTCCTGATTG AGACAAGCTC CAGAGGTGTT 
CGACTCAAAG GCTGTCCCAA TGAACCCTAC TTTGGTTGCC TCTCAGCGCT GGTTTATCAA 
CACTCAATGA CCCCGCTAGC TCTGCCCTGT AAGCTGATGA TCCCCACCAA AGACCCGAAT 
GAGGAAGCGC TGGAGTTGGC CACACCCACT GACCCAGTTG TCGAGCTTCT GAAGCAAGGA 
GCAGTTCAGA AGGTTCCAGA GGAGGCTTAC GCGTGTAACG TCCTCTACAT CAACTCGGTG 
GACATGGAGT CTCTCACGGG GCCCCAGGCC ATCGCCAAGG CGATCAGCCT GACTTTGGCG 
ACAAACCCCC TGCCGGCCGC CACCACCGTC CACTTCAAAG TGTCTATGCA GGGCATCACG 
CTCACCGACA GCCAGAGGAA ACTTTTTTTC AGACGCCACT ATCCCATCAA CACCATAACG 
TTCTGTGACA TTGACCCGCA GAACAGGAAA TGGGGAAAAG AAGGAGGCGG TTCAGTGAAG 
CTGTTTGGTT TTGTGGCACG GAAACAAGGA AGCACAACCG ACAACGTGAG CCACCTGTTC 
GCAGAGCTGG ACCCAGATCA GCCGGCTTCC GCCATCACCA GTTTTGTCTC CAAGACGATG 
AAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840195.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696055.1 Takifugu rubripes tensin (LOC101064247), transcript variant X7, mRNA.

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CloneID	OTo71059
Clone ID Related Accession (Same CDS sequence)	XM_029840195.1
Accession Version	XM_029840195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGGCGTTC GCTCCAGAGG AATGACCCCA GAAGTGACAC AGGACAGCGG TCGACGTAGG 
ACCACCAGTG AGGGACAGTA CCAGAGCAGC CACGACAGCA CACCAATGGT TGATTCACCG 
GACTTTGCCC ACAGTTTGAC CCTCAACCCT GGAGGCAGAC CCAGAGAGGG CCAGATTCAC 
AGCTACAGGG AGGTGTTGGA GGACAGCGAG GTGGACCTTG TCATGCACAG TCCCACCTTC 
AGAGGCGGTG GTGAGAATTC CCCTCAGACC CCCAAGTTCC CTGTGTCACC ACAGACTCCT 
TACTTTAACA TGTCTCGTGT CCCTCCAGGT CTGGCCAAGA CTCCTCTGTC AGCACTGGAA 
CTGAAGCCCC ATGATCACGA CTCTGATTCA AATGAGGGAG AGCAAGATAA CCACACTTCC 
AGGGACTCAA GAGTTCAACC CTTCAACACC TCTTTCTCTT CCACCTACCC TAGTCACAGC 
AGTGATGGAA GAAGAACCGG TTCTTCAGAC GTTCACAGCC ACACCCCCTC CTACGCAAAC 
AGACCCACCT CCGCTGAGGG CTCCCAGGTC AACGTCATGG GAGTCCACAC AGTGCCCGGC 
AGTCCCAACA CCCTCCATCG CACCGTAGCC ACCAACACTC CACCAAGCCC GGCCCTTCAG 
AGACGTCTGG GTCAAGCCAG TCCCTCACTA GCCAGACACC AACCCCCAGC TGGTGCCAAC 
AGGGACAGTG TCCCCTCCAG TCCTCTGATT GGCCGCAACC CTAAAACAGC AGCTGCTGGC 
GTGCCCCCCA GCCCTCTAAT GGGCCGCCGA ACGGCGATGA GCGGCCACAG CACGCCTGAT 
GACATCGGCC CACCACCTTC TACGCCTGCC TTCCCTGTGT CCCCACAGCT ACCAGAAAAG 
AGGCACATGT CCACTGGAGA CGTGGAGAGG ACAGATAACA AGAACCTTAT GGCCGGTGTT 
GGCAGCAGTG CGCCAAACCT GTCTGGCACG CACACGTTGA CAGACGTCCA AAAGTCCATC 
TATGATGGGT ATCCGGACAT TAAGATGAAT GTGAAGTTTG TCCAAGACAC ATCCAAGTTC 
TGGTACAAGC CAGACATCTC CAGAGAGCAA GCCATCAATC TGTTGAAGGA CAGAGAGCCC 
GGGGCCTTCA TCATAAGGGA CAGCCATTCC TTCCGTGGAG CTTACGGCCT TGCAATGAAG 
GTGGCCTGCC CGCCGCCAAC TGTCCAGCAG AACAAAAAAG TTGGAGACTT GACCAACGAG 
CTTGTGAGAC ACTTCCTGAT TGAGACAAGC TCCAGAGGTG TTCGACTCAA AGGCTGTCCC 
AATGAACCCT ACTTTGGTTG CCTCTCAGCG CTGGTTTATC AACACTCAAT GACCCCGCTA 
GCTCTGCCCT GTAAGCTGAT GATCCCCACC AAAGACCCGA ATGAGGAAGC GCTGGAGTTG 
GCCACACCCA CTGACCCAGT TGTCGAGCTT CTGAAGCAAG GAGCAGTTCA GAAGGTTCCA 
GAGGAGGCTT ACGCGTGTAA CGTCCTCTAC ATCAACTCGG TGGACATGGA GTCTCTCACG 
GGGCCCCAGG CCATCGCCAA GGCGATCAGC CTGACTTTGG CGACAAACCC CCTGCCGGCC 
GCCACCACCG TCCACTTCAA AGTGTCTATG CAGGGCATCA CGCTCACCGA CAGCCAGAGG 
AAACTTTTTT TCAGACGCCA CTATCCCATC AACACCATAA CGTTCTGTGA CATTGACCCG 
CAGAACAGGA AATGGGGAAA AGAAGGAGGC GGTTCAGTGA AGCTGTTTGG TTTTGTGGCA 
CGGAAACAAG GAAGCACAAC CGACAACGTG AGCCACCTGT TCGCAGAGCT GGACCCAGAT 
CAGCCGGCTT CCGCCATCAC CAGTTTTGTC TCCAAGACGA TGAAGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696055.1
CDS	643..5673
Translation	MARLNWCLAAVISWGKFLFACFFPLRGAKRDTPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELPPTPQLPLKHVDTPGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840195.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840195.1

ORF Insert Sequence:

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGGCGTTC GCTCCAGAGG AATGACCCCA GAAGTGACAC AGGACAGCGG TCGACGTAGG 
ACCACCAGTG AGGGACAGTA CCAGAGCAGC CACGACAGCA CACCAATGGT TGATTCACCG 
GACTTTGCCC ACAGTTTGAC CCTCAACCCT GGAGGCAGAC CCAGAGAGGG CCAGATTCAC 
AGCTACAGGG AGGTGTTGGA GGACAGCGAG GTGGACCTTG TCATGCACAG TCCCACCTTC 
AGAGGCGGTG GTGAGAATTC CCCTCAGACC CCCAAGTTCC CTGTGTCACC ACAGACTCCT 
TACTTTAACA TGTCTCGTGT CCCTCCAGGT CTGGCCAAGA CTCCTCTGTC AGCACTGGAA 
CTGAAGCCCC ATGATCACGA CTCTGATTCA AATGAGGGAG AGCAAGATAA CCACACTTCC 
AGGGACTCAA GAGTTCAACC CTTCAACACC TCTTTCTCTT CCACCTACCC TAGTCACAGC 
AGTGATGGAA GAAGAACCGG TTCTTCAGAC GTTCACAGCC ACACCCCCTC CTACGCAAAC 
AGACCCACCT CCGCTGAGGG CTCCCAGGTC AACGTCATGG GAGTCCACAC AGTGCCCGGC 
AGTCCCAACA CCCTCCATCG CACCGTAGCC ACCAACACTC CACCAAGCCC GGCCCTTCAG 
AGACGTCTGG GTCAAGCCAG TCCCTCACTA GCCAGACACC AACCCCCAGC TGGTGCCAAC 
AGGGACAGTG TCCCCTCCAG TCCTCTGATT GGCCGCAACC CTAAAACAGC AGCTGCTGGC 
GTGCCCCCCA GCCCTCTAAT GGGCCGCCGA ACGGCGATGA GCGGCCACAG CACGCCTGAT 
GACATCGGCC CACCACCTTC TACGCCTGCC TTCCCTGTGT CCCCACAGCT ACCAGAAAAG 
AGGCACATGT CCACTGGAGA CGTGGAGAGG ACAGATAACA AGAACCTTAT GGCCGGTGTT 
GGCAGCAGTG CGCCAAACCT GTCTGGCACG CACACGTTGA CAGACGTCCA AAAGTCCATC 
TATGATGGGT ATCCGGACAT TAAGATGAAT GTGAAGTTTG TCCAAGACAC ATCCAAGTTC 
TGGTACAAGC CAGACATCTC CAGAGAGCAA GCCATCAATC TGTTGAAGGA CAGAGAGCCC 
GGGGCCTTCA TCATAAGGGA CAGCCATTCC TTCCGTGGAG CTTACGGCCT TGCAATGAAG 
GTGGCCTGCC CGCCGCCAAC TGTCCAGCAG AACAAAAAAG TTGGAGACTT GACCAACGAG 
CTTGTGAGAC ACTTCCTGAT TGAGACAAGC TCCAGAGGTG TTCGACTCAA AGGCTGTCCC 
AATGAACCCT ACTTTGGTTG CCTCTCAGCG CTGGTTTATC AACACTCAAT GACCCCGCTA 
GCTCTGCCCT GTAAGCTGAT GATCCCCACC AAAGACCCGA ATGAGGAAGC GCTGGAGTTG 
GCCACACCCA CTGACCCAGT TGTCGAGCTT CTGAAGCAAG GAGCAGTTCA GAAGGTTCCA 
GAGGAGGCTT ACGCGTGTAA CGTCCTCTAC ATCAACTCGG TGGACATGGA GTCTCTCACG 
GGGCCCCAGG CCATCGCCAA GGCGATCAGC CTGACTTTGG CGACAAACCC CCTGCCGGCC 
GCCACCACCG TCCACTTCAA AGTGTCTATG CAGGGCATCA CGCTCACCGA CAGCCAGAGG 
AAACTTTTTT TCAGACGCCA CTATCCCATC AACACCATAA CGTTCTGTGA CATTGACCCG 
CAGAACAGGA AATGGGGAAA AGAAGGAGGC GGTTCAGTGA AGCTGTTTGG TTTTGTGGCA 
CGGAAACAAG GAAGCACAAC CGACAACGTG AGCCACCTGT TCGCAGAGCT GGACCCAGAT 
CAGCCGGCTT CCGCCATCAC CAGTTTTGTC TCCAAGACGA TGAAGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840196.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696056.1 Takifugu rubripes tensin (LOC101064247), transcript variant X8, mRNA.

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CloneID	OTo71060
Clone ID Related Accession (Same CDS sequence)	XM_029840196.1
Accession Version	XM_029840196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGAGTACA ACGCTCGTAT CCGGGGCATC AGCGAAAGCA TGACACCGCA ACAATCTGAC 
CGTCACCGCT CCTATTCCTT CTCTGGCGTT CGCTCCAGAG GAATGACCCC AGAAGTGACA 
CAGGACAGCG GTCGACGTAG GACCACCAGT GAGGGACAGT ACCAGAGCAG CCACGACAGC 
ACACCAATGG TTGATTCACC GGACTTTGCC CACAGTTTGA CCCTCAACCC TGGAGGCAGA 
CCCAGAGAGG GCCAGATTCA CAGCTACAGG GAGGTGTTGG AGGACAGCGA GGTGGACCTT 
GTCATGCACA GTCCCACCTT CAGAGGCGGT GCTCGTGTCC CTCCAGGTCT GGCCAAGACT 
CCTCTGTCAG CACTGGAACT GAAGCCCCAT GATCACGATA ACCACACTTC CAGGGACTCA 
AGAGTTCAAC CCTTCAACAC CTCTTTCTCT TCCACCTACC CTAGTCACAG CAGTGATGGA 
AGAAGAACCG GTTCTTCAGA CGTTCACAGC CACACCCCCT CCTACGCAAA CAGACCCACC 
TCCGCTGAGG GCTCCCAGGT CAACGTCATG GGAGTCCACA CAGTGCCCGG CAGTCCCAAC 
ACCCTCCATC GCACCGTAGC CACCAACACT CCACCAAGCC CGGCCCTTCA GAGACGTCTG 
GGTCAAGCCA GTCCCTCACT AGCCAGACAC CAACCCCCAG CTGGTGCCAA CAGGGACAGT 
GTCCCCTCCA GTCCTCTGAT TGGCCGCAAC CCTAAAACAG CAGCTGCTGG CGTGCCCCCC 
AGCCCTCTAA TGGGCCGCCG AACGGCGATG AGCGGCCACA GCACGCCTGA TGACATCGGC 
CCACCACCTT CTACGCCTGC CTTCCCTGTG TCCCCACAGC TACCAGAAAA GAGGCACATG 
TCCACTGGAG ACGTGGAGAG GACAGATAAC AAGAACCTTA TGGCCGGTGT TGGCAGCAGT 
GCGCCAAACC TGTCTGGCAC GCACACGTTG ACAGACGTCC AAAAGTCCAT CTATGATGGG 
TATCCGGACA TTAAGATGAA TGTGAAGTTT GTCCAAGACA CATCCAAGTT CTGGTACAAG 
CCAGACATCT CCAGAGAGCA AGCCATCAAT CTGTTGAAGG ACAGAGAGCC CGGGGCCTTC 
ATCATAAGGG ACAGCCATTC CTTCCGTGGA GCTTACGGCC TTGCAATGAA GGTGGCCTGC 
CCGCCGCCAA CTGTCCAGCA GAACAAAAAA GTTGGAGACT TGACCAACGA GCTTGTGAGA 
CACTTCCTGA TTGAGACAAG CTCCAGAGGT GTTCGACTCA AAGGCTGTCC CAATGAACCC 
TACTTTGGTT GCCTCTCAGC GCTGGTTTAT CAACACTCAA TGACCCCGCT AGCTCTGCCC 
TGTAAGCTGA TGATCCCCAC CAAAGACCCG AATGAGGAAG CGCTGGAGTT GGCCACACCC 
ACTGACCCAG TTGTCGAGCT TCTGAAGCAA GGAGCAGTTC AGAAGGTTCC AGAGGAGGCT 
TACGCGTGTA ACGTCCTCTA CATCAACTCG GTGGACATGG AGTCTCTCAC GGGGCCCCAG 
GCCATCGCCA AGGCGATCAG CCTGACTTTG GCGACAAACC CCCTGCCGGC CGCCACCACC 
GTCCACTTCA AAGTGTCTAT GCAGGGCATC ACGCTCACCG ACAGCCAGAG GAAACTTTTT 
TTCAGACGCC ACTATCCCAT CAACACCATA ACGTTCTGTG ACATTGACCC GCAGAACAGG 
AAATGGGGAA AAGAAGGAGG CGGTTCAGTG AAGCTGTTTG GTTTTGTGGC ACGGAAACAA 
GGAAGCACAA CCGACAACGT GAGCCACCTG TTCGCAGAGC TGGACCCAGA TCAGCCGGCT 
TCCGCCATCA CCAGTTTTGT CTCCAAGACG ATGAAGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696056.1
CDS	647..5668
Translation	MARLNWCLAAVISWGKFLFACFFPLRGAKRDTPDELEGVHTHTFKLKPFKKAKSCDICKQAITKEGLVCKACRLSCHKKCEVKVTTSCQTSTNHELPPTPQLPLKHVDTPGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGARVPPGLAKTPLSALELKPHDHDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840196.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840196.1

ORF Insert Sequence:

ATGGCTCGCC TCAACTGGTG CCTGGCTGCC GTCATCAGCT GGGGCAAGTT CCTCTTCGCC 
TGCTTCTTCC CACTGCGGGG GGCCAAGCGA GACACGCCGG ATGAGCTGGA GGGCGTCCAC 
ACTCACACCT TCAAGCTGAA GCCATTCAAG AAGGCCAAAA GCTGTGACAT CTGTAAGCAG 
GCCATCACCA AGGAGGGCCT CGTCTGCAAA GCGTGTCGGC TCTCCTGCCA CAAAAAATGT 
GAAGTGAAGG TCACCACATC ATGTCAGACG TCCACCAACC ATGAGCTGCC CCCCACTCCG 
CAGCTGCCAT TAAAGCATGT AGACACGCCG GGATCTGCGA GGTCCTGCAA GAGTGTGGAG 
ATAAGGCGGA AGCAGTCTCG AAGCCAGAGT GTGGTTCTGA CCATGGAGGA GAACTATGAG 
GTAGACCTGG TCTACATCAC GGAGAGGATC ATCTCCCTCT GCTTCCCCAT TGGCATGGAG 
GACCAGGGCT ACATCGCCAA CCTCAAGGAG GTGGCCACCA TGCTGCGATC CAAACATGGA 
GATCATTATT TGGTGCTGAA CCTGAGTGAA TGGAGGACTG AGTTATCGAA GCTAAACCAC 
AAGGTCTTGG AATTTGGCTG GCCGGACTTT CACGCACCAG CCCTGGACAA GATCTGTAGC 
ATGTGCAAAG CGATTGACAC GTGGCTCAAT GGAGACTCGC ACAATGTGGT GGTGCTTCAC 
AACAAGGGGA ACCGAGGTCG AACTGGGGTC GTGGTGGCTG CTTACATGCA CTATAGCAAC 
ATATCAGCAA GTGCTGACCA GGCCTTGGAC AGGTTCGCCA TGAGACGGTT CTACGAGGAT 
AAAGCACTTC CTGTGGGTCA GCCGTCACAA AAAAGGTATG TGCGATACTT CAATGGCCTT 
CTGTCGGGGC ACATCAAAAT CAACAACAAA CCTCTCTTCC TGCACCACGT CATCATGCAC 
GGTATCCCCA ACTTTGAGTC TAAAGGAGGC TGCCGCCCTT TCCTGAAGAT CTACCAAGCG 
ATGCAGCCAG TCTACACGTC AGGGATATAC AACGTGCAGG GAGACAGTAA CACTAGTATC 
TGCATCACCA TCGAACCAGG CCTCCTCCTG AAGGGAGACA TCCTGCTGAA GTGTTACCAC 
AAACGATACA GAGGCCCGAC CAGAGACGTA GTGTTCCGGG TGCAGTTCCA CACCTGTGCC 
ATCCACGACC TTGGCGTGGT GTTCGGGAAG AGCGAACTGG ACGAGACCTT CAAAGACGAC 
AGGTTTCCTG AATACGGGAA AGTGGAGTTT GTTTTCTCCT ATGCACCAGA AAAAATTAGA 
GGCTTAGACC ACCTGGAGAA CGGCCCCAGT GTTTCCGTGG ACTACAACAC CCAGGAAAAC 
CTGATTCGCT GTGATTCATA TGACAACTTT AGCCAGCGCT CTGAAGATGC CGTGGATGGA 
GAGCGTGAGG TCATTCACAC TCAAGGTCCA CTTGATGGAA GCCTTTATGC CCGGGTCTGT 
AAGAAAGACT CCCTGGAGGG AGTTGTCGCC ATCAACGGTA ATCCAGTCCA TGAAAATTCC 
TTAGCCAGCA CAGAGAACCA GTTACAACAC ACAAGCTCCT TGCTTCAAAC CGCCGAACAG 
ACCCTTCCTG TGATTGGCCA CGCTTCTCTT CCTGCTGTTG ACCACACACT CTCTGTGAGC 
AGTGACTCGG GCAACTCCAC TGCATCCATT AAAACTGATC GTACCGACGA GCACAGCCAG 
CCAGTGCACA GCACTGTCAA CCACAACAAT CCGGCAGTTC CCCACCCGAC ACTTAGTCCC 
CAGGAGAAAA AAGAGCTTGA TCAGCTCCTT AGTGGCCTCG AAGCACCGAC TCTTCAACGA 
CAAGCCTACC TGGCTACATC CACCAGTCCG GGAGGCGGTG TCCGACACCT GGTCCCAGCA 
CAAGTGCACG TTAACGGAGG CCACCCAAGG CTAGTTCCTG TCCCCTCAAC GGAAGAGCGT 
GAGACGGACA TTCTTGACGA CGAGCTGCCA AATAGCCAAG AGGGAAACAG TGTGGACAGC 
CTGGGTACAC TGTCATCCTT AGAGGGTCAA GCAACCCCAG GGGATCTCTA TTACCAACAA 
GAGAAGTCCG TCAGCAGACA AAACGACGGG CCATATCTTG AGAGAGGCGT TTGTCGGGAA 
AAAAAAAACG AGATGCCCAT GCATGGAGTT CATACTCCGA TGATGCAGGA AAGACCTGTG 
GACTCTGTAT CCCCACAAGG GGGATATAAC AAATACCAGA ACGGTGGTGG GATGTACCAC 
TCTGAATCCT ATGGGATTCC ACCTGCCAGC AGCCCGGAGG GAAACCCTAA ACTGATGCCC 
AGGGCCCCAG AGAGAAGCAC AAGTAGCCGG GAGGCTGTTC AAAGGGGCCT CAATGCCTGG 
CACCAGTATA GCCTCCCAGA TGATCCATTT GGACCTCCCC TGCAGTCAAC CCATAGCATG 
CCACAGTTCC CCACAGCAGC CTCACAGCGG GACATAGAGC AGTCCATCGA GGCACTGAAT 
ATGCTCATGC TTGACCTGGA CCCCATTAAC TCTCACATGT CCAAGTCCCA TAGTGCACCC 
TCCGGGGAGA ACATCCTGAA TTCATCCCAG GCACCCTTCT CCCAGACCCT CTCCAGACCC 
TCATACCAAG GAGACTCCGC CATCCACAGA TACAATAACT CTGAATCTGT CAACAATCCC 
TCCCATCAGC CCATATGGTC AGCTGGAAGA GTGTCAGCAG TGCCTAACCA GAGCCCTATT 
ATGGATTCTC AAGCACATTT GCCCCAGAGA TCCAGCTCGA GCTGTCAACA CCAGAAAACC 
ACCCCAACAC ACACCCCAGA GCCCAATCTC CATGCACGAC AAGGAGCCAT CCATTACCCC 
ATTGATCCCT CCTTTAATTT CAACCAGCAT CTCCCAGTTA GGCCTGCAAA CACTTTCCCA 
GGTGTTTCCC CCTCCCCAGA CCTGCAGGGT TCGTCTCCAT ACCAGGGCTT CAGTGCCTCT 
TCCTCACCCC TTCCTGCGCT CACTCCACAG CCAAAGAACA CATCGTCTTC ATCCCTGCCT 
CAAGAACAAG ATGTTGAGGA AGAGGCGCTG AACTTGGAAG GTCTGGTGGC TCATCGCATT 
GCCGAGTACA ACGCTCGTAT CCGGGGCATC AGCGAAAGCA TGACACCGCA ACAATCTGAC 
CGTCACCGCT CCTATTCCTT CTCTGGCGTT CGCTCCAGAG GAATGACCCC AGAAGTGACA 
CAGGACAGCG GTCGACGTAG GACCACCAGT GAGGGACAGT ACCAGAGCAG CCACGACAGC 
ACACCAATGG TTGATTCACC GGACTTTGCC CACAGTTTGA CCCTCAACCC TGGAGGCAGA 
CCCAGAGAGG GCCAGATTCA CAGCTACAGG GAGGTGTTGG AGGACAGCGA GGTGGACCTT 
GTCATGCACA GTCCCACCTT CAGAGGCGGT GCTCGTGTCC CTCCAGGTCT GGCCAAGACT 
CCTCTGTCAG CACTGGAACT GAAGCCCCAT GATCACGATA ACCACACTTC CAGGGACTCA 
AGAGTTCAAC CCTTCAACAC CTCTTTCTCT TCCACCTACC CTAGTCACAG CAGTGATGGA 
AGAAGAACCG GTTCTTCAGA CGTTCACAGC CACACCCCCT CCTACGCAAA CAGACCCACC 
TCCGCTGAGG GCTCCCAGGT CAACGTCATG GGAGTCCACA CAGTGCCCGG CAGTCCCAAC 
ACCCTCCATC GCACCGTAGC CACCAACACT CCACCAAGCC CGGCCCTTCA GAGACGTCTG 
GGTCAAGCCA GTCCCTCACT AGCCAGACAC CAACCCCCAG CTGGTGCCAA CAGGGACAGT 
GTCCCCTCCA GTCCTCTGAT TGGCCGCAAC CCTAAAACAG CAGCTGCTGG CGTGCCCCCC 
AGCCCTCTAA TGGGCCGCCG AACGGCGATG AGCGGCCACA GCACGCCTGA TGACATCGGC 
CCACCACCTT CTACGCCTGC CTTCCCTGTG TCCCCACAGC TACCAGAAAA GAGGCACATG 
TCCACTGGAG ACGTGGAGAG GACAGATAAC AAGAACCTTA TGGCCGGTGT TGGCAGCAGT 
GCGCCAAACC TGTCTGGCAC GCACACGTTG ACAGACGTCC AAAAGTCCAT CTATGATGGG 
TATCCGGACA TTAAGATGAA TGTGAAGTTT GTCCAAGACA CATCCAAGTT CTGGTACAAG 
CCAGACATCT CCAGAGAGCA AGCCATCAAT CTGTTGAAGG ACAGAGAGCC CGGGGCCTTC 
ATCATAAGGG ACAGCCATTC CTTCCGTGGA GCTTACGGCC TTGCAATGAA GGTGGCCTGC 
CCGCCGCCAA CTGTCCAGCA GAACAAAAAA GTTGGAGACT TGACCAACGA GCTTGTGAGA 
CACTTCCTGA TTGAGACAAG CTCCAGAGGT GTTCGACTCA AAGGCTGTCC CAATGAACCC 
TACTTTGGTT GCCTCTCAGC GCTGGTTTAT CAACACTCAA TGACCCCGCT AGCTCTGCCC 
TGTAAGCTGA TGATCCCCAC CAAAGACCCG AATGAGGAAG CGCTGGAGTT GGCCACACCC 
ACTGACCCAG TTGTCGAGCT TCTGAAGCAA GGAGCAGTTC AGAAGGTTCC AGAGGAGGCT 
TACGCGTGTA ACGTCCTCTA CATCAACTCG GTGGACATGG AGTCTCTCAC GGGGCCCCAG 
GCCATCGCCA AGGCGATCAG CCTGACTTTG GCGACAAACC CCCTGCCGGC CGCCACCACC 
GTCCACTTCA AAGTGTCTAT GCAGGGCATC ACGCTCACCG ACAGCCAGAG GAAACTTTTT 
TTCAGACGCC ACTATCCCAT CAACACCATA ACGTTCTGTG ACATTGACCC GCAGAACAGG 
AAATGGGGAA AAGAAGGAGG CGGTTCAGTG AAGCTGTTTG GTTTTGTGGC ACGGAAACAA 
GGAAGCACAA CCGACAACGT GAGCCACCTG TTCGCAGAGC TGGACCCAGA TCAGCCGGCT 
TCCGCCATCA CCAGTTTTGT CTCCAAGACG ATGAAGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840197.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696057.1 Takifugu rubripes tensin (LOC101064247), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OTo71061
Clone ID Related Accession (Same CDS sequence)	XM_029840197.1
Accession Version	XM_029840197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG GAAACTCAGT AGAGAAAAGC TTTTCTCACC TGCGTTCATC CTTGAGATGG 
AGCACCGGTC ACCTGTTCAG CAAGGGATCT GCGAGGTCCT GCAAGAGTGT GGAGATAAGG 
CGGAAGCAGT CTCGAAGCCA GAGTGTGGTT CTGACCATGG AGGAGAACTA TGAGGTAGAC 
CTGGTCTACA TCACGGAGAG GATCATCTCC CTCTGCTTCC CCATTGGCAT GGAGGACCAG 
GGCTACATCG CCAACCTCAA GGAGGTGGCC ACCATGCTGC GATCCAAACA TGGAGATCAT 
TATTTGGTGC TGAACCTGAG TGAATGGAGG ACTGAGTTAT CGAAGCTAAA CCACAAGGTC 
TTGGAATTTG GCTGGCCGGA CTTTCACGCA CCAGCCCTGG ACAAGATCTG TAGCATGTGC 
AAAGCGATTG ACACGTGGCT CAATGGAGAC TCGCACAATG TGGTGGTGCT TCACAACAAG 
GGGAACCGAG GTCGAACTGG GGTCGTGGTG GCTGCTTACA TGCACTATAG CAACATATCA 
GCAAGTGCTG ACCAGGCCTT GGACAGGTTC GCCATGAGAC GGTTCTACGA GGATAAAGCA 
CTTCCTGTGG GTCAGCCGTC ACAAAAAAGG TATGTGCGAT ACTTCAATGG CCTTCTGTCG 
GGGCACATCA AAATCAACAA CAAACCTCTC TTCCTGCACC ACGTCATCAT GCACGGTATC 
CCCAACTTTG AGTCTAAAGG AGGCTGCCGC CCTTTCCTGA AGATCTACCA AGCGATGCAG 
CCAGTCTACA CGTCAGGGAT ATACAACGTG CAGGGAGACA GTAACACTAG TATCTGCATC 
ACCATCGAAC CAGGCCTCCT CCTGAAGGGA GACATCCTGC TGAAGTGTTA CCACAAACGA 
TACAGAGGCC CGACCAGAGA CGTAGTGTTC CGGGTGCAGT TCCACACCTG TGCCATCCAC 
GACCTTGGCG TGGTGTTCGG GAAGAGCGAA CTGGACGAGA CCTTCAAAGA CGACAGGTTT 
CCTGAATACG GGAAAGTGGA GTTTGTTTTC TCCTATGCAC CAGAAAAAAT TAGAGGCTTA 
GACCACCTGG AGAACGGCCC CAGTGTTTCC GTGGACTACA ACACCCAGGA AAACCTGATT 
CGCTGTGATT CATATGACAA CTTTAGCCAG CGCTCTGAAG ATGCCGTGGA TGGAGAGCGT 
GAGGTCATTC ACACTCAAGG TCCACTTGAT GGAAGCCTTT ATGCCCGGGT CTGTAAGAAA 
GACTCCCTGG AGGGAGTTGT CGCCATCAAC GGTAATCCAG TCCATGAAAA TTCCTTAGCC 
AGCACAGAGA ACCAGTTACA ACACACAAGC TCCTTGCTTC AAACCGCCGA ACAGACCCTT 
CCTGTGATTG GCCACGCTTC TCTTCCTGCT GTTGACCACA CACTCTCTGT GAGCAGTGAC 
TCGGGCAACT CCACTGCATC CATTAAAACT GATCGTACCG ACGAGCACAG CCAGCCAGTG 
CACAGCACTG TCAACCACAA CAATCCGGCA GTTCCCCACC CGACACTTAG TCCCCAGGAG 
AAAAAAGAGC TTGATCAGCT CCTTAGTGGC CTCGAAGCAC CGACTCTTCA ACGACAAGCC 
TACCTGGCTA CATCCACCAG TCCGGGAGGC GGTGTCCGAC ACCTGGTCCC AGCACAAGTG 
CACGTTAACG GAGGCCACCC AAGGCTAGTT CCTGTCCCCT CAACGGAAGA GCGTGAGACG 
GACATTCTTG ACGACGAGCT GCCAAATAGC CAAGAGGGAA ACAGTGTGGA CAGCCTGGGT 
ACACTGTCAT CCTTAGAGGG TCAAGCAACC CCAGGGGATC TCTATTACCA ACAAGAGAAG 
TCCGTCAGCA GACAAAACGA CGGGCCATAT CTTGAGAGAG GCGTTTGTCG GGAAAAAAAA 
AACGAGATGC CCATGCATGG AGTTCATACT CCGATGATGC AGGAAAGACC TGTGGACTCT 
GTATCCCCAC AAGGGGGATA TAACAAATAC CAGAACGGTG GTGGGATGTA CCACTCTGAA 
TCCTATGGGA TTCCACCTGC CAGCAGCCCG GAGGGAAACC CTAAACTGAT GCCCAGGGCC 
CCAGAGAGAA GCACAAGTAG CCGGGAGGCT GTTCAAAGGG GCCTCAATGC CTGGCACCAG 
TATAGCCTCC CAGATGATCC ATTTGGACCT CCCCTGCAGT CAACCCATAG CATGCCACAG 
TTCCCCACAG CAGCCTCACA GCGGGACATA GAGCAGTCCA TCGAGGCACT GAATATGCTC 
ATGCTTGACC TGGACCCCAT TAACTCTCAC ATGTCCAAGT CCCATAGTGC ACCCTCCGGG 
GAGAACATCC TGAATTCATC CCAGGCACCC TTCTCCCAGA CCCTCTCCAG ACCCTCATAC 
CAAGGAGACT CCGCCATCCA CAGATACAAT AACTCTGAAT CTGTCAACAA TCCCTCCCAT 
CAGCCCATAT GGTCAGCTGG AAGAGTGTCA GCAGTGCCTA ACCAGAGCCC TATTATGGAT 
TCTCAAGCAC ATTTGCCCCA GAGATCCAGC TCGAGCTGTC AACACCAGAA AACCACCCCA 
ACACACACCC CAGAGCCCAA TCTCCATGCA CGACAAGGAG CCATCCATTA CCCCATTGAT 
CCCTCCTTTA ATTTCAACCA GCATCTCCCA GTTAGGCCTG CAAACACTTT CCCAGGTGTT 
TCCCCCTCCC CAGACCTGCA GGGTTCGTCT CCATACCAGG GCTTCAGTGC CTCTTCCTCA 
CCCCTTCCTG CGCTCACTCC ACAGCCAAAG AACACATCGT CTTCATCCCT GCCTCAAGAA 
CAAGATGTTG AGGAAGAGGC GCTGAACTTG GAAGGTCTGG TGGCTCATCG CATTGCCGAG 
TACAACGCTC GTATCCGGGG CATCAGCGAA AGCATGACAC CGCAACAATC TGACCGTCAC 
CGCTCCTATT CCTTCTCTGG CGTTCGCTCC AGAGGAATGA CCCCAGAAGT GACACAGGAC 
AGCGGTCGAC GTAGGACCAC CAGTGAGGGA CAGTACCAGA GCAGCCACGA CAGCACACCA 
ATGGTTGATT CACCGGACTT TGCCCACAGT TTGACCCTCA ACCCTGGAGG CAGACCCAGA 
GAGGGCCAGA TTCACAGCTA CAGGGAGGTG TTGGAGGACA GCGAGGTGGA CCTTGTCATG 
CACAGTCCCA CCTTCAGAGG CGGTGGTGAG AATTCCCCTC AGACCCCCAA GTTCCCTGTG 
TCACCACAGA CTCCTTACTT TAACATGTCT CGTGTCCCTC CAGGTCTGGC CAAGACTCCT 
CTGTCAGCAC TGGAACTGAA GCCCCATGAT CACGACTCTG ATTCAAATGA GGGAGAGCAA 
GATAACCACA CTTCCAGGGA CTCAAGAGTT CAACCCTTCA ACACCTCTTT CTCTTCCACC 
TACCCTAGTC ACAGCAGTGA TGGAAGAAGA ACCGGTTCTT CAGACGTTCA CAGCCACACC 
CCCTCCTACG CAAACAGACC CACCTCCGCT GAGGGCTCCC AGGTCAACGT CATGGGAGTC 
CACACAGTGC CCGGCAGTCC CAACACCCTC CATCGCACCG TAGCCACCAA CACTCCACCA 
AGCCCGGCCC TTCAGAGACG TCTGGGTCAA GCCAGTCCCT CACTAGCCAG ACACCAACCC 
CCAGCTGGTG CCAACAGGGA CAGTGTCCCC TCCAGTCCTC TGATTGGCCG CAACCCTAAA 
ACAGCAGCTG CTGGCGTGCC CCCCAGCCCT CTAATGGGCC GCCGAACGGC GATGAGCGGC 
CACAGCACGC CTGATGACAT CGGCCCACCA CCTTCTACGC CTGCCTTCCC TGTGTCCCCA 
CAGCTACCAG AAAAGAGGCA CATGTCCACT GGAGACGTGG AGAGGACAGA TAACAAGAAC 
CTTATGGCCG GTGTTGGCAG CAGTGCGCCA AACCTGTCTG GCACGCACAC GTTGACAGAC 
GTCCAAAAGT CCATCTATGA TGGGTATCCG GACATTAAGA TGAATGTGAA GTTTGTCCAA 
GACACATCCA AGTTCTGGTA CAAGCCAGAC ATCTCCAGAG AGCAAGCCAT CAATCTGTTG 
AAGGACAGAG AGCCCGGGGC CTTCATCATA AGGGACAGCC ATTCCTTCCG TGGAGCTTAC 
GGCCTTGCAA TGAAGGTGGC CTGCCCGCCG CCAACTGTCC AGCAGAACAA AAAAGTTGGA 
GACTTGACCA ACGAGCTTGT GAGACACTTC CTGATTGAGA CAAGCTCCAG AGGTGTTCGA 
CTCAAAGGCT GTCCCAATGA ACCCTACTTT GGTTGCCTCT CAGCGCTGGT TTATCAACAC 
TCAATGACCC CGCTAGCTCT GCCCTGTAAG CTGATGATCC CCACCAAAGA CCCGAATGAG 
GAAGCGCTGG AGTTGGCCAC ACCCACTGAC CCAGTTGTCG AGCTTCTGAA GCAAGGAGCA 
GTTCAGAAGG TTCCAGAGGA GGCTTACGCG TGTAACGTCC TCTACATCAA CTCGGTGGAC 
ATGGAGTCTC TCACGGGGCC CCAGGCCATC GCCAAGGCGA TCAGCCTGAC TTTGGCGACA 
AACCCCCTGC CGGCCGCCAC CACCGTCCAC TTCAAAGTGT CTATGCAGGG CATCACGCTC 
ACCGACAGCC AGAGGAAACT TTTTTTCAGA CGCCACTATC CCATCAACAC CATAACGTTC 
TGTGACATTG ACCCGCAGAA CAGGAAATGG GGAAAAGAAG GAGGCGGTTC AGTGAAGCTG 
TTTGGTTTTG TGGCACGGAA ACAAGGAAGC ACAACCGACA ACGTGAGCCA CCTGTTCGCA 
GAGCTGGACC CAGATCAGCC GGCTTCCGCC ATCACCAGTT TTGTCTCCAA GACGATGAAG 
CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696057.1
CDS	920..5785
Translation	MSLGNSVEKSFSHLRSSLRWSTGHLFSKGSARSCKSVEIRRKQSRSQSVVLTMEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840197.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840197.1

ORF Insert Sequence:

ATGTCTCTGG GAAACTCAGT AGAGAAAAGC TTTTCTCACC TGCGTTCATC CTTGAGATGG 
AGCACCGGTC ACCTGTTCAG CAAGGGATCT GCGAGGTCCT GCAAGAGTGT GGAGATAAGG 
CGGAAGCAGT CTCGAAGCCA GAGTGTGGTT CTGACCATGG AGGAGAACTA TGAGGTAGAC 
CTGGTCTACA TCACGGAGAG GATCATCTCC CTCTGCTTCC CCATTGGCAT GGAGGACCAG 
GGCTACATCG CCAACCTCAA GGAGGTGGCC ACCATGCTGC GATCCAAACA TGGAGATCAT 
TATTTGGTGC TGAACCTGAG TGAATGGAGG ACTGAGTTAT CGAAGCTAAA CCACAAGGTC 
TTGGAATTTG GCTGGCCGGA CTTTCACGCA CCAGCCCTGG ACAAGATCTG TAGCATGTGC 
AAAGCGATTG ACACGTGGCT CAATGGAGAC TCGCACAATG TGGTGGTGCT TCACAACAAG 
GGGAACCGAG GTCGAACTGG GGTCGTGGTG GCTGCTTACA TGCACTATAG CAACATATCA 
GCAAGTGCTG ACCAGGCCTT GGACAGGTTC GCCATGAGAC GGTTCTACGA GGATAAAGCA 
CTTCCTGTGG GTCAGCCGTC ACAAAAAAGG TATGTGCGAT ACTTCAATGG CCTTCTGTCG 
GGGCACATCA AAATCAACAA CAAACCTCTC TTCCTGCACC ACGTCATCAT GCACGGTATC 
CCCAACTTTG AGTCTAAAGG AGGCTGCCGC CCTTTCCTGA AGATCTACCA AGCGATGCAG 
CCAGTCTACA CGTCAGGGAT ATACAACGTG CAGGGAGACA GTAACACTAG TATCTGCATC 
ACCATCGAAC CAGGCCTCCT CCTGAAGGGA GACATCCTGC TGAAGTGTTA CCACAAACGA 
TACAGAGGCC CGACCAGAGA CGTAGTGTTC CGGGTGCAGT TCCACACCTG TGCCATCCAC 
GACCTTGGCG TGGTGTTCGG GAAGAGCGAA CTGGACGAGA CCTTCAAAGA CGACAGGTTT 
CCTGAATACG GGAAAGTGGA GTTTGTTTTC TCCTATGCAC CAGAAAAAAT TAGAGGCTTA 
GACCACCTGG AGAACGGCCC CAGTGTTTCC GTGGACTACA ACACCCAGGA AAACCTGATT 
CGCTGTGATT CATATGACAA CTTTAGCCAG CGCTCTGAAG ATGCCGTGGA TGGAGAGCGT 
GAGGTCATTC ACACTCAAGG TCCACTTGAT GGAAGCCTTT ATGCCCGGGT CTGTAAGAAA 
GACTCCCTGG AGGGAGTTGT CGCCATCAAC GGTAATCCAG TCCATGAAAA TTCCTTAGCC 
AGCACAGAGA ACCAGTTACA ACACACAAGC TCCTTGCTTC AAACCGCCGA ACAGACCCTT 
CCTGTGATTG GCCACGCTTC TCTTCCTGCT GTTGACCACA CACTCTCTGT GAGCAGTGAC 
TCGGGCAACT CCACTGCATC CATTAAAACT GATCGTACCG ACGAGCACAG CCAGCCAGTG 
CACAGCACTG TCAACCACAA CAATCCGGCA GTTCCCCACC CGACACTTAG TCCCCAGGAG 
AAAAAAGAGC TTGATCAGCT CCTTAGTGGC CTCGAAGCAC CGACTCTTCA ACGACAAGCC 
TACCTGGCTA CATCCACCAG TCCGGGAGGC GGTGTCCGAC ACCTGGTCCC AGCACAAGTG 
CACGTTAACG GAGGCCACCC AAGGCTAGTT CCTGTCCCCT CAACGGAAGA GCGTGAGACG 
GACATTCTTG ACGACGAGCT GCCAAATAGC CAAGAGGGAA ACAGTGTGGA CAGCCTGGGT 
ACACTGTCAT CCTTAGAGGG TCAAGCAACC CCAGGGGATC TCTATTACCA ACAAGAGAAG 
TCCGTCAGCA GACAAAACGA CGGGCCATAT CTTGAGAGAG GCGTTTGTCG GGAAAAAAAA 
AACGAGATGC CCATGCATGG AGTTCATACT CCGATGATGC AGGAAAGACC TGTGGACTCT 
GTATCCCCAC AAGGGGGATA TAACAAATAC CAGAACGGTG GTGGGATGTA CCACTCTGAA 
TCCTATGGGA TTCCACCTGC CAGCAGCCCG GAGGGAAACC CTAAACTGAT GCCCAGGGCC 
CCAGAGAGAA GCACAAGTAG CCGGGAGGCT GTTCAAAGGG GCCTCAATGC CTGGCACCAG 
TATAGCCTCC CAGATGATCC ATTTGGACCT CCCCTGCAGT CAACCCATAG CATGCCACAG 
TTCCCCACAG CAGCCTCACA GCGGGACATA GAGCAGTCCA TCGAGGCACT GAATATGCTC 
ATGCTTGACC TGGACCCCAT TAACTCTCAC ATGTCCAAGT CCCATAGTGC ACCCTCCGGG 
GAGAACATCC TGAATTCATC CCAGGCACCC TTCTCCCAGA CCCTCTCCAG ACCCTCATAC 
CAAGGAGACT CCGCCATCCA CAGATACAAT AACTCTGAAT CTGTCAACAA TCCCTCCCAT 
CAGCCCATAT GGTCAGCTGG AAGAGTGTCA GCAGTGCCTA ACCAGAGCCC TATTATGGAT 
TCTCAAGCAC ATTTGCCCCA GAGATCCAGC TCGAGCTGTC AACACCAGAA AACCACCCCA 
ACACACACCC CAGAGCCCAA TCTCCATGCA CGACAAGGAG CCATCCATTA CCCCATTGAT 
CCCTCCTTTA ATTTCAACCA GCATCTCCCA GTTAGGCCTG CAAACACTTT CCCAGGTGTT 
TCCCCCTCCC CAGACCTGCA GGGTTCGTCT CCATACCAGG GCTTCAGTGC CTCTTCCTCA 
CCCCTTCCTG CGCTCACTCC ACAGCCAAAG AACACATCGT CTTCATCCCT GCCTCAAGAA 
CAAGATGTTG AGGAAGAGGC GCTGAACTTG GAAGGTCTGG TGGCTCATCG CATTGCCGAG 
TACAACGCTC GTATCCGGGG CATCAGCGAA AGCATGACAC CGCAACAATC TGACCGTCAC 
CGCTCCTATT CCTTCTCTGG CGTTCGCTCC AGAGGAATGA CCCCAGAAGT GACACAGGAC 
AGCGGTCGAC GTAGGACCAC CAGTGAGGGA CAGTACCAGA GCAGCCACGA CAGCACACCA 
ATGGTTGATT CACCGGACTT TGCCCACAGT TTGACCCTCA ACCCTGGAGG CAGACCCAGA 
GAGGGCCAGA TTCACAGCTA CAGGGAGGTG TTGGAGGACA GCGAGGTGGA CCTTGTCATG 
CACAGTCCCA CCTTCAGAGG CGGTGGTGAG AATTCCCCTC AGACCCCCAA GTTCCCTGTG 
TCACCACAGA CTCCTTACTT TAACATGTCT CGTGTCCCTC CAGGTCTGGC CAAGACTCCT 
CTGTCAGCAC TGGAACTGAA GCCCCATGAT CACGACTCTG ATTCAAATGA GGGAGAGCAA 
GATAACCACA CTTCCAGGGA CTCAAGAGTT CAACCCTTCA ACACCTCTTT CTCTTCCACC 
TACCCTAGTC ACAGCAGTGA TGGAAGAAGA ACCGGTTCTT CAGACGTTCA CAGCCACACC 
CCCTCCTACG CAAACAGACC CACCTCCGCT GAGGGCTCCC AGGTCAACGT CATGGGAGTC 
CACACAGTGC CCGGCAGTCC CAACACCCTC CATCGCACCG TAGCCACCAA CACTCCACCA 
AGCCCGGCCC TTCAGAGACG TCTGGGTCAA GCCAGTCCCT CACTAGCCAG ACACCAACCC 
CCAGCTGGTG CCAACAGGGA CAGTGTCCCC TCCAGTCCTC TGATTGGCCG CAACCCTAAA 
ACAGCAGCTG CTGGCGTGCC CCCCAGCCCT CTAATGGGCC GCCGAACGGC GATGAGCGGC 
CACAGCACGC CTGATGACAT CGGCCCACCA CCTTCTACGC CTGCCTTCCC TGTGTCCCCA 
CAGCTACCAG AAAAGAGGCA CATGTCCACT GGAGACGTGG AGAGGACAGA TAACAAGAAC 
CTTATGGCCG GTGTTGGCAG CAGTGCGCCA AACCTGTCTG GCACGCACAC GTTGACAGAC 
GTCCAAAAGT CCATCTATGA TGGGTATCCG GACATTAAGA TGAATGTGAA GTTTGTCCAA 
GACACATCCA AGTTCTGGTA CAAGCCAGAC ATCTCCAGAG AGCAAGCCAT CAATCTGTTG 
AAGGACAGAG AGCCCGGGGC CTTCATCATA AGGGACAGCC ATTCCTTCCG TGGAGCTTAC 
GGCCTTGCAA TGAAGGTGGC CTGCCCGCCG CCAACTGTCC AGCAGAACAA AAAAGTTGGA 
GACTTGACCA ACGAGCTTGT GAGACACTTC CTGATTGAGA CAAGCTCCAG AGGTGTTCGA 
CTCAAAGGCT GTCCCAATGA ACCCTACTTT GGTTGCCTCT CAGCGCTGGT TTATCAACAC 
TCAATGACCC CGCTAGCTCT GCCCTGTAAG CTGATGATCC CCACCAAAGA CCCGAATGAG 
GAAGCGCTGG AGTTGGCCAC ACCCACTGAC CCAGTTGTCG AGCTTCTGAA GCAAGGAGCA 
GTTCAGAAGG TTCCAGAGGA GGCTTACGCG TGTAACGTCC TCTACATCAA CTCGGTGGAC 
ATGGAGTCTC TCACGGGGCC CCAGGCCATC GCCAAGGCGA TCAGCCTGAC TTTGGCGACA 
AACCCCCTGC CGGCCGCCAC CACCGTCCAC TTCAAAGTGT CTATGCAGGG CATCACGCTC 
ACCGACAGCC AGAGGAAACT TTTTTTCAGA CGCCACTATC CCATCAACAC CATAACGTTC 
TGTGACATTG ACCCGCAGAA CAGGAAATGG GGAAAAGAAG GAGGCGGTTC AGTGAAGCTG 
TTTGGTTTTG TGGCACGGAA ACAAGGAAGC ACAACCGACA ACGTGAGCCA CCTGTTCGCA 
GAGCTGGACC CAGATCAGCC GGCTTCCGCC ATCACCAGTT TTGTCTCCAA GACGATGAAG 
CGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840200.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696060.1 Takifugu rubripes tensin (LOC101064247), transcript variant X11, mRNA.

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CloneID	OTo71062
Clone ID Related Accession (Same CDS sequence)	XM_029840200.1 , XM_029840199.1 , XM_029840201.1
Accession Version	XM_029840200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACTATGAGGT AGACCTGGTC TACATCACGG AGAGGATCAT CTCCCTCTGC 
TTCCCCATTG GCATGGAGGA CCAGGGCTAC ATCGCCAACC TCAAGGAGGT GGCCACCATG 
CTGCGATCCA AACATGGAGA TCATTATTTG GTGCTGAACC TGAGTGAATG GAGGACTGAG 
TTATCGAAGC TAAACCACAA GGTCTTGGAA TTTGGCTGGC CGGACTTTCA CGCACCAGCC 
CTGGACAAGA TCTGTAGCAT GTGCAAAGCG ATTGACACGT GGCTCAATGG AGACTCGCAC 
AATGTGGTGG TGCTTCACAA CAAGGGGAAC CGAGGTCGAA CTGGGGTCGT GGTGGCTGCT 
TACATGCACT ATAGCAACAT ATCAGCAAGT GCTGACCAGG CCTTGGACAG GTTCGCCATG 
AGACGGTTCT ACGAGGATAA AGCACTTCCT GTGGGTCAGC CGTCACAAAA AAGGTATGTG 
CGATACTTCA ATGGCCTTCT GTCGGGGCAC ATCAAAATCA ACAACAAACC TCTCTTCCTG 
CACCACGTCA TCATGCACGG TATCCCCAAC TTTGAGTCTA AAGGAGGCTG CCGCCCTTTC 
CTGAAGATCT ACCAAGCGAT GCAGCCAGTC TACACGTCAG GGATATACAA CGTGCAGGGA 
GACAGTAACA CTAGTATCTG CATCACCATC GAACCAGGCC TCCTCCTGAA GGGAGACATC 
CTGCTGAAGT GTTACCACAA ACGATACAGA GGCCCGACCA GAGACGTAGT GTTCCGGGTG 
CAGTTCCACA CCTGTGCCAT CCACGACCTT GGCGTGGTGT TCGGGAAGAG CGAACTGGAC 
GAGACCTTCA AAGACGACAG GTTTCCTGAA TACGGGAAAG TGGAGTTTGT TTTCTCCTAT 
GCACCAGAAA AAATTAGAGG CTTAGACCAC CTGGAGAACG GCCCCAGTGT TTCCGTGGAC 
TACAACACCC AGGAAAACCT GATTCGCTGT GATTCATATG ACAACTTTAG CCAGCGCTCT 
GAAGATGCCG TGGATGGAGA GCGTGAGGTC ATTCACACTC AAGGTCCACT TGATGGAAGC 
CTTTATGCCC GGGTCTGTAA GAAAGACTCC CTGGAGGGAG TTGTCGCCAT CAACGGTAAT 
CCAGTCCATG AAAATTCCTT AGCCAGCACA GAGAACCAGT TACAACACAC AAGCTCCTTG 
CTTCAAACCG CCGAACAGAC CCTTCCTGTG ATTGGCCACG CTTCTCTTCC TGCTGTTGAC 
CACACACTCT CTGTGAGCAG TGACTCGGGC AACTCCACTG CATCCATTAA AACTGATCGT 
ACCGACGAGC ACAGCCAGCC AGTGCACAGC ACTGTCAACC ACAACAATCC GGCAGTTCCC 
CACCCGACAC TTAGTCCCCA GGAGAAAAAA GAGCTTGATC AGCTCCTTAG TGGCCTCGAA 
GCACCGACTC TTCAACGACA AGCCTACCTG GCTACATCCA CCAGTCCGGG AGGCGGTGTC 
CGACACCTGG TCCCAGCACA AGTGCACGTT AACGGAGGCC ACCCAAGGCT AGTTCCTGTC 
CCCTCAACGG AAGAGCGTGA GACGGACATT CTTGACGACG AGCTGCCAAA TAGCCAAGAG 
GGAAACAGTG TGGACAGCCT GGGTACACTG TCATCCTTAG AGGGTCAAGC AACCCCAGGG 
GATCTCTATT ACCAACAAGA GAAGTCCGTC AGCAGACAAA ACGACGGGCC ATATCTTGAG 
AGAGGCGTTT GTCGGGAAAA AAAAAACGAG ATGCCCATGC ATGGAGTTCA TACTCCGATG 
ATGCAGGAAA GACCTGTGGA CTCTGTATCC CCACAAGGGG GATATAACAA ATACCAGAAC 
GGTGGTGGGA TGTACCACTC TGAATCCTAT GGGATTCCAC CTGCCAGCAG CCCGGAGGGA 
AACCCTAAAC TGATGCCCAG GGCCCCAGAG AGAAGCACAA GTAGCCGGGA GGCTGTTCAA 
AGGGGCCTCA ATGCCTGGCA CCAGTATAGC CTCCCAGATG ATCCATTTGG ACCTCCCCTG 
CAGTCAACCC ATAGCATGCC ACAGTTCCCC ACAGCAGCCT CACAGCGGGA CATAGAGCAG 
TCCATCGAGG CACTGAATAT GCTCATGCTT GACCTGGACC CCATTAACTC TCACATGTCC 
AAGTCCCATA GTGCACCCTC CGGGGAGAAC ATCCTGAATT CATCCCAGGC ACCCTTCTCC 
CAGACCCTCT CCAGACCCTC ATACCAAGGA GACTCCGCCA TCCACAGATA CAATAACTCT 
GAATCTGTCA ACAATCCCTC CCATCAGCCC ATATGGTCAG CTGGAAGAGT GTCAGCAGTG 
CCTAACCAGA GCCCTATTAT GGATTCTCAA GCACATTTGC CCCAGAGATC CAGCTCGAGC 
TGTCAACACC AGAAAACCAC CCCAACACAC ACCCCAGAGC CCAATCTCCA TGCACGACAA 
GGAGCCATCC ATTACCCCAT TGATCCCTCC TTTAATTTCA ACCAGCATCT CCCAGTTAGG 
CCTGCAAACA CTTTCCCAGG TGTTTCCCCC TCCCCAGACC TGCAGGGTTC GTCTCCATAC 
CAGGGCTTCA GTGCCTCTTC CTCACCCCTT CCTGCGCTCA CTCCACAGCC AAAGAACACA 
TCGTCTTCAT CCCTGCCTCA AGAACAAGAT GTTGAGGAAG AGGCGCTGAA CTTGGAAGGT 
CTGGTGGCTC ATCGCATTGC CGAGTACAAC GCTCGTATCC GGGGCATCAG CGAAAGCATG 
ACACCGCAAC AATCTGACCG TCACCGCTCC TATTCCTTCT CTGGCGTTCG CTCCAGAGGA 
ATGACCCCAG AAGTGACACA GGACAGCGGT CGACGTAGGA CCACCAGTGA GGGACAGTAC 
CAGAGCAGCC ACGACAGCAC ACCAATGGTT GATTCACCGG ACTTTGCCCA CAGTTTGACC 
CTCAACCCTG GAGGCAGACC CAGAGAGGGC CAGATTCACA GCTACAGGGA GGTGTTGGAG 
GACAGCGAGG TGGACCTTGT CATGCACAGT CCCACCTTCA GAGGCGGTGG TGAGAATTCC 
CCTCAGACCC CCAAGTTCCC TGTGTCACCA CAGACTCCTT ACTTTAACAT GTCTCGTGTC 
CCTCCAGGTC TGGCCAAGAC TCCTCTGTCA GCACTGGAAC TGAAGCCCCA TGATCACGAC 
TCTGATTCAA ATGAGGGAGA GCAAGATAAC CACACTTCCA GGGACTCAAG AGTTCAACCC 
TTCAACACCT CTTTCTCTTC CACCTACCCT AGTCACAGCA GTGATGGAAG AAGAACCGGT 
TCTTCAGACG TTCACAGCCA CACCCCCTCC TACGCAAACA GACCCACCTC CGCTGAGGGC 
TCCCAGGTCA ACGTCATGGG AGTCCACACA GTGCCCGGCA GTCCCAACAC CCTCCATCGC 
ACCGTAGCCA CCAACACTCC ACCAAGCCCG GCCCTTCAGA GACGTCTGGG TCAAGCCAGT 
CCCTCACTAG CCAGACACCA ACCCCCAGCT GGTGCCAACA GGGACAGTGT CCCCTCCAGT 
CCTCTGATTG GCCGCAACCC TAAAACAGCA GCTGCTGGCG TGCCCCCCAG CCCTCTAATG 
GGCCGCCGAA CGGCGATGAG CGGCCACAGC ACGCCTGATG ACATCGGCCC ACCACCTTCT 
ACGCCTGCCT TCCCTGTGTC CCCACAGCTA CCAGAAAAGA GGCACATGTC CACTGGAGAC 
GTGGAGAGGA CAGATAACAA GAACCTTATG GCCGGTGTTG GCAGCAGTGC GCCAAACCTG 
TCTGGCACGC ACACGTTGAC AGACGTCCAA AAGTCCATCT ATGATGGGTA TCCGGACATT 
AAGATGAATG TGAAGTTTGT CCAAGACACA TCCAAGTTCT GGTACAAGCC AGACATCTCC 
AGAGAGCAAG CCATCAATCT GTTGAAGGAC AGAGAGCCCG GGGCCTTCAT CATAAGGGAC 
AGCCATTCCT TCCGTGGAGC TTACGGCCTT GCAATGAAGG TGGCCTGCCC GCCGCCAACT 
GTCCAGCAGA ACAAAAAAGT TGGAGACTTG ACCAACGAGC TTGTGAGACA CTTCCTGATT 
GAGACAAGCT CCAGAGGTGT TCGACTCAAA GGCTGTCCCA ATGAACCCTA CTTTGGTTGC 
CTCTCAGCGC TGGTTTATCA ACACTCAATG ACCCCGCTAG CTCTGCCCTG TAAGCTGATG 
ATCCCCACCA AAGACCCGAA TGAGGAAGCG CTGGAGTTGG CCACACCCAC TGACCCAGTT 
GTCGAGCTTC TGAAGCAAGG AGCAGTTCAG AAGGTTCCAG AGGAGGCTTA CGCGTGTAAC 
GTCCTCTACA TCAACTCGGT GGACATGGAG TCTCTCACGG GGCCCCAGGC CATCGCCAAG 
GCGATCAGCC TGACTTTGGC GACAAACCCC CTGCCGGCCG CCACCACCGT CCACTTCAAA 
GTGTCTATGC AGGGCATCAC GCTCACCGAC AGCCAGAGGA AACTTTTTTT CAGACGCCAC 
TATCCCATCA ACACCATAAC GTTCTGTGAC ATTGACCCGC AGAACAGGAA ATGGGGAAAA 
GAAGGAGGCG GTTCAGTGAA GCTGTTTGGT TTTGTGGCAC GGAAACAAGG AAGCACAACC 
GACAACGTGA GCCACCTGTT CGCAGAGCTG GACCCAGATC AGCCGGCTTC CGCCATCACC 
AGTTTTGTCT CCAAGACGAT GAAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696060.1
CDS	425..5134
Translation	MEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840200.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840200.1

ORF Insert Sequence:

ATGGAGGAGA ACTATGAGGT AGACCTGGTC TACATCACGG AGAGGATCAT CTCCCTCTGC 
TTCCCCATTG GCATGGAGGA CCAGGGCTAC ATCGCCAACC TCAAGGAGGT GGCCACCATG 
CTGCGATCCA AACATGGAGA TCATTATTTG GTGCTGAACC TGAGTGAATG GAGGACTGAG 
TTATCGAAGC TAAACCACAA GGTCTTGGAA TTTGGCTGGC CGGACTTTCA CGCACCAGCC 
CTGGACAAGA TCTGTAGCAT GTGCAAAGCG ATTGACACGT GGCTCAATGG AGACTCGCAC 
AATGTGGTGG TGCTTCACAA CAAGGGGAAC CGAGGTCGAA CTGGGGTCGT GGTGGCTGCT 
TACATGCACT ATAGCAACAT ATCAGCAAGT GCTGACCAGG CCTTGGACAG GTTCGCCATG 
AGACGGTTCT ACGAGGATAA AGCACTTCCT GTGGGTCAGC CGTCACAAAA AAGGTATGTG 
CGATACTTCA ATGGCCTTCT GTCGGGGCAC ATCAAAATCA ACAACAAACC TCTCTTCCTG 
CACCACGTCA TCATGCACGG TATCCCCAAC TTTGAGTCTA AAGGAGGCTG CCGCCCTTTC 
CTGAAGATCT ACCAAGCGAT GCAGCCAGTC TACACGTCAG GGATATACAA CGTGCAGGGA 
GACAGTAACA CTAGTATCTG CATCACCATC GAACCAGGCC TCCTCCTGAA GGGAGACATC 
CTGCTGAAGT GTTACCACAA ACGATACAGA GGCCCGACCA GAGACGTAGT GTTCCGGGTG 
CAGTTCCACA CCTGTGCCAT CCACGACCTT GGCGTGGTGT TCGGGAAGAG CGAACTGGAC 
GAGACCTTCA AAGACGACAG GTTTCCTGAA TACGGGAAAG TGGAGTTTGT TTTCTCCTAT 
GCACCAGAAA AAATTAGAGG CTTAGACCAC CTGGAGAACG GCCCCAGTGT TTCCGTGGAC 
TACAACACCC AGGAAAACCT GATTCGCTGT GATTCATATG ACAACTTTAG CCAGCGCTCT 
GAAGATGCCG TGGATGGAGA GCGTGAGGTC ATTCACACTC AAGGTCCACT TGATGGAAGC 
CTTTATGCCC GGGTCTGTAA GAAAGACTCC CTGGAGGGAG TTGTCGCCAT CAACGGTAAT 
CCAGTCCATG AAAATTCCTT AGCCAGCACA GAGAACCAGT TACAACACAC AAGCTCCTTG 
CTTCAAACCG CCGAACAGAC CCTTCCTGTG ATTGGCCACG CTTCTCTTCC TGCTGTTGAC 
CACACACTCT CTGTGAGCAG TGACTCGGGC AACTCCACTG CATCCATTAA AACTGATCGT 
ACCGACGAGC ACAGCCAGCC AGTGCACAGC ACTGTCAACC ACAACAATCC GGCAGTTCCC 
CACCCGACAC TTAGTCCCCA GGAGAAAAAA GAGCTTGATC AGCTCCTTAG TGGCCTCGAA 
GCACCGACTC TTCAACGACA AGCCTACCTG GCTACATCCA CCAGTCCGGG AGGCGGTGTC 
CGACACCTGG TCCCAGCACA AGTGCACGTT AACGGAGGCC ACCCAAGGCT AGTTCCTGTC 
CCCTCAACGG AAGAGCGTGA GACGGACATT CTTGACGACG AGCTGCCAAA TAGCCAAGAG 
GGAAACAGTG TGGACAGCCT GGGTACACTG TCATCCTTAG AGGGTCAAGC AACCCCAGGG 
GATCTCTATT ACCAACAAGA GAAGTCCGTC AGCAGACAAA ACGACGGGCC ATATCTTGAG 
AGAGGCGTTT GTCGGGAAAA AAAAAACGAG ATGCCCATGC ATGGAGTTCA TACTCCGATG 
ATGCAGGAAA GACCTGTGGA CTCTGTATCC CCACAAGGGG GATATAACAA ATACCAGAAC 
GGTGGTGGGA TGTACCACTC TGAATCCTAT GGGATTCCAC CTGCCAGCAG CCCGGAGGGA 
AACCCTAAAC TGATGCCCAG GGCCCCAGAG AGAAGCACAA GTAGCCGGGA GGCTGTTCAA 
AGGGGCCTCA ATGCCTGGCA CCAGTATAGC CTCCCAGATG ATCCATTTGG ACCTCCCCTG 
CAGTCAACCC ATAGCATGCC ACAGTTCCCC ACAGCAGCCT CACAGCGGGA CATAGAGCAG 
TCCATCGAGG CACTGAATAT GCTCATGCTT GACCTGGACC CCATTAACTC TCACATGTCC 
AAGTCCCATA GTGCACCCTC CGGGGAGAAC ATCCTGAATT CATCCCAGGC ACCCTTCTCC 
CAGACCCTCT CCAGACCCTC ATACCAAGGA GACTCCGCCA TCCACAGATA CAATAACTCT 
GAATCTGTCA ACAATCCCTC CCATCAGCCC ATATGGTCAG CTGGAAGAGT GTCAGCAGTG 
CCTAACCAGA GCCCTATTAT GGATTCTCAA GCACATTTGC CCCAGAGATC CAGCTCGAGC 
TGTCAACACC AGAAAACCAC CCCAACACAC ACCCCAGAGC CCAATCTCCA TGCACGACAA 
GGAGCCATCC ATTACCCCAT TGATCCCTCC TTTAATTTCA ACCAGCATCT CCCAGTTAGG 
CCTGCAAACA CTTTCCCAGG TGTTTCCCCC TCCCCAGACC TGCAGGGTTC GTCTCCATAC 
CAGGGCTTCA GTGCCTCTTC CTCACCCCTT CCTGCGCTCA CTCCACAGCC AAAGAACACA 
TCGTCTTCAT CCCTGCCTCA AGAACAAGAT GTTGAGGAAG AGGCGCTGAA CTTGGAAGGT 
CTGGTGGCTC ATCGCATTGC CGAGTACAAC GCTCGTATCC GGGGCATCAG CGAAAGCATG 
ACACCGCAAC AATCTGACCG TCACCGCTCC TATTCCTTCT CTGGCGTTCG CTCCAGAGGA 
ATGACCCCAG AAGTGACACA GGACAGCGGT CGACGTAGGA CCACCAGTGA GGGACAGTAC 
CAGAGCAGCC ACGACAGCAC ACCAATGGTT GATTCACCGG ACTTTGCCCA CAGTTTGACC 
CTCAACCCTG GAGGCAGACC CAGAGAGGGC CAGATTCACA GCTACAGGGA GGTGTTGGAG 
GACAGCGAGG TGGACCTTGT CATGCACAGT CCCACCTTCA GAGGCGGTGG TGAGAATTCC 
CCTCAGACCC CCAAGTTCCC TGTGTCACCA CAGACTCCTT ACTTTAACAT GTCTCGTGTC 
CCTCCAGGTC TGGCCAAGAC TCCTCTGTCA GCACTGGAAC TGAAGCCCCA TGATCACGAC 
TCTGATTCAA ATGAGGGAGA GCAAGATAAC CACACTTCCA GGGACTCAAG AGTTCAACCC 
TTCAACACCT CTTTCTCTTC CACCTACCCT AGTCACAGCA GTGATGGAAG AAGAACCGGT 
TCTTCAGACG TTCACAGCCA CACCCCCTCC TACGCAAACA GACCCACCTC CGCTGAGGGC 
TCCCAGGTCA ACGTCATGGG AGTCCACACA GTGCCCGGCA GTCCCAACAC CCTCCATCGC 
ACCGTAGCCA CCAACACTCC ACCAAGCCCG GCCCTTCAGA GACGTCTGGG TCAAGCCAGT 
CCCTCACTAG CCAGACACCA ACCCCCAGCT GGTGCCAACA GGGACAGTGT CCCCTCCAGT 
CCTCTGATTG GCCGCAACCC TAAAACAGCA GCTGCTGGCG TGCCCCCCAG CCCTCTAATG 
GGCCGCCGAA CGGCGATGAG CGGCCACAGC ACGCCTGATG ACATCGGCCC ACCACCTTCT 
ACGCCTGCCT TCCCTGTGTC CCCACAGCTA CCAGAAAAGA GGCACATGTC CACTGGAGAC 
GTGGAGAGGA CAGATAACAA GAACCTTATG GCCGGTGTTG GCAGCAGTGC GCCAAACCTG 
TCTGGCACGC ACACGTTGAC AGACGTCCAA AAGTCCATCT ATGATGGGTA TCCGGACATT 
AAGATGAATG TGAAGTTTGT CCAAGACACA TCCAAGTTCT GGTACAAGCC AGACATCTCC 
AGAGAGCAAG CCATCAATCT GTTGAAGGAC AGAGAGCCCG GGGCCTTCAT CATAAGGGAC 
AGCCATTCCT TCCGTGGAGC TTACGGCCTT GCAATGAAGG TGGCCTGCCC GCCGCCAACT 
GTCCAGCAGA ACAAAAAAGT TGGAGACTTG ACCAACGAGC TTGTGAGACA CTTCCTGATT 
GAGACAAGCT CCAGAGGTGT TCGACTCAAA GGCTGTCCCA ATGAACCCTA CTTTGGTTGC 
CTCTCAGCGC TGGTTTATCA ACACTCAATG ACCCCGCTAG CTCTGCCCTG TAAGCTGATG 
ATCCCCACCA AAGACCCGAA TGAGGAAGCG CTGGAGTTGG CCACACCCAC TGACCCAGTT 
GTCGAGCTTC TGAAGCAAGG AGCAGTTCAG AAGGTTCCAG AGGAGGCTTA CGCGTGTAAC 
GTCCTCTACA TCAACTCGGT GGACATGGAG TCTCTCACGG GGCCCCAGGC CATCGCCAAG 
GCGATCAGCC TGACTTTGGC GACAAACCCC CTGCCGGCCG CCACCACCGT CCACTTCAAA 
GTGTCTATGC AGGGCATCAC GCTCACCGAC AGCCAGAGGA AACTTTTTTT CAGACGCCAC 
TATCCCATCA ACACCATAAC GTTCTGTGAC ATTGACCCGC AGAACAGGAA ATGGGGAAAA 
GAAGGAGGCG GTTCAGTGAA GCTGTTTGGT TTTGTGGCAC GGAAACAAGG AAGCACAACC 
GACAACGTGA GCCACCTGTT CGCAGAGCTG GACCCAGATC AGCCGGCTTC CGCCATCACC 
AGTTTTGTCT CCAAGACGAT GAAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840199.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696059.1 Takifugu rubripes tensin (LOC101064247), transcript variant X10, mRNA.

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CloneID	OTo71062
Clone ID Related Accession (Same CDS sequence)	XM_029840200.1 , XM_029840199.1 , XM_029840201.1
Accession Version	XM_029840199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACTATGAGGT AGACCTGGTC TACATCACGG AGAGGATCAT CTCCCTCTGC 
TTCCCCATTG GCATGGAGGA CCAGGGCTAC ATCGCCAACC TCAAGGAGGT GGCCACCATG 
CTGCGATCCA AACATGGAGA TCATTATTTG GTGCTGAACC TGAGTGAATG GAGGACTGAG 
TTATCGAAGC TAAACCACAA GGTCTTGGAA TTTGGCTGGC CGGACTTTCA CGCACCAGCC 
CTGGACAAGA TCTGTAGCAT GTGCAAAGCG ATTGACACGT GGCTCAATGG AGACTCGCAC 
AATGTGGTGG TGCTTCACAA CAAGGGGAAC CGAGGTCGAA CTGGGGTCGT GGTGGCTGCT 
TACATGCACT ATAGCAACAT ATCAGCAAGT GCTGACCAGG CCTTGGACAG GTTCGCCATG 
AGACGGTTCT ACGAGGATAA AGCACTTCCT GTGGGTCAGC CGTCACAAAA AAGGTATGTG 
CGATACTTCA ATGGCCTTCT GTCGGGGCAC ATCAAAATCA ACAACAAACC TCTCTTCCTG 
CACCACGTCA TCATGCACGG TATCCCCAAC TTTGAGTCTA AAGGAGGCTG CCGCCCTTTC 
CTGAAGATCT ACCAAGCGAT GCAGCCAGTC TACACGTCAG GGATATACAA CGTGCAGGGA 
GACAGTAACA CTAGTATCTG CATCACCATC GAACCAGGCC TCCTCCTGAA GGGAGACATC 
CTGCTGAAGT GTTACCACAA ACGATACAGA GGCCCGACCA GAGACGTAGT GTTCCGGGTG 
CAGTTCCACA CCTGTGCCAT CCACGACCTT GGCGTGGTGT TCGGGAAGAG CGAACTGGAC 
GAGACCTTCA AAGACGACAG GTTTCCTGAA TACGGGAAAG TGGAGTTTGT TTTCTCCTAT 
GCACCAGAAA AAATTAGAGG CTTAGACCAC CTGGAGAACG GCCCCAGTGT TTCCGTGGAC 
TACAACACCC AGGAAAACCT GATTCGCTGT GATTCATATG ACAACTTTAG CCAGCGCTCT 
GAAGATGCCG TGGATGGAGA GCGTGAGGTC ATTCACACTC AAGGTCCACT TGATGGAAGC 
CTTTATGCCC GGGTCTGTAA GAAAGACTCC CTGGAGGGAG TTGTCGCCAT CAACGGTAAT 
CCAGTCCATG AAAATTCCTT AGCCAGCACA GAGAACCAGT TACAACACAC AAGCTCCTTG 
CTTCAAACCG CCGAACAGAC CCTTCCTGTG ATTGGCCACG CTTCTCTTCC TGCTGTTGAC 
CACACACTCT CTGTGAGCAG TGACTCGGGC AACTCCACTG CATCCATTAA AACTGATCGT 
ACCGACGAGC ACAGCCAGCC AGTGCACAGC ACTGTCAACC ACAACAATCC GGCAGTTCCC 
CACCCGACAC TTAGTCCCCA GGAGAAAAAA GAGCTTGATC AGCTCCTTAG TGGCCTCGAA 
GCACCGACTC TTCAACGACA AGCCTACCTG GCTACATCCA CCAGTCCGGG AGGCGGTGTC 
CGACACCTGG TCCCAGCACA AGTGCACGTT AACGGAGGCC ACCCAAGGCT AGTTCCTGTC 
CCCTCAACGG AAGAGCGTGA GACGGACATT CTTGACGACG AGCTGCCAAA TAGCCAAGAG 
GGAAACAGTG TGGACAGCCT GGGTACACTG TCATCCTTAG AGGGTCAAGC AACCCCAGGG 
GATCTCTATT ACCAACAAGA GAAGTCCGTC AGCAGACAAA ACGACGGGCC ATATCTTGAG 
AGAGGCGTTT GTCGGGAAAA AAAAAACGAG ATGCCCATGC ATGGAGTTCA TACTCCGATG 
ATGCAGGAAA GACCTGTGGA CTCTGTATCC CCACAAGGGG GATATAACAA ATACCAGAAC 
GGTGGTGGGA TGTACCACTC TGAATCCTAT GGGATTCCAC CTGCCAGCAG CCCGGAGGGA 
AACCCTAAAC TGATGCCCAG GGCCCCAGAG AGAAGCACAA GTAGCCGGGA GGCTGTTCAA 
AGGGGCCTCA ATGCCTGGCA CCAGTATAGC CTCCCAGATG ATCCATTTGG ACCTCCCCTG 
CAGTCAACCC ATAGCATGCC ACAGTTCCCC ACAGCAGCCT CACAGCGGGA CATAGAGCAG 
TCCATCGAGG CACTGAATAT GCTCATGCTT GACCTGGACC CCATTAACTC TCACATGTCC 
AAGTCCCATA GTGCACCCTC CGGGGAGAAC ATCCTGAATT CATCCCAGGC ACCCTTCTCC 
CAGACCCTCT CCAGACCCTC ATACCAAGGA GACTCCGCCA TCCACAGATA CAATAACTCT 
GAATCTGTCA ACAATCCCTC CCATCAGCCC ATATGGTCAG CTGGAAGAGT GTCAGCAGTG 
CCTAACCAGA GCCCTATTAT GGATTCTCAA GCACATTTGC CCCAGAGATC CAGCTCGAGC 
TGTCAACACC AGAAAACCAC CCCAACACAC ACCCCAGAGC CCAATCTCCA TGCACGACAA 
GGAGCCATCC ATTACCCCAT TGATCCCTCC TTTAATTTCA ACCAGCATCT CCCAGTTAGG 
CCTGCAAACA CTTTCCCAGG TGTTTCCCCC TCCCCAGACC TGCAGGGTTC GTCTCCATAC 
CAGGGCTTCA GTGCCTCTTC CTCACCCCTT CCTGCGCTCA CTCCACAGCC AAAGAACACA 
TCGTCTTCAT CCCTGCCTCA AGAACAAGAT GTTGAGGAAG AGGCGCTGAA CTTGGAAGGT 
CTGGTGGCTC ATCGCATTGC CGAGTACAAC GCTCGTATCC GGGGCATCAG CGAAAGCATG 
ACACCGCAAC AATCTGACCG TCACCGCTCC TATTCCTTCT CTGGCGTTCG CTCCAGAGGA 
ATGACCCCAG AAGTGACACA GGACAGCGGT CGACGTAGGA CCACCAGTGA GGGACAGTAC 
CAGAGCAGCC ACGACAGCAC ACCAATGGTT GATTCACCGG ACTTTGCCCA CAGTTTGACC 
CTCAACCCTG GAGGCAGACC CAGAGAGGGC CAGATTCACA GCTACAGGGA GGTGTTGGAG 
GACAGCGAGG TGGACCTTGT CATGCACAGT CCCACCTTCA GAGGCGGTGG TGAGAATTCC 
CCTCAGACCC CCAAGTTCCC TGTGTCACCA CAGACTCCTT ACTTTAACAT GTCTCGTGTC 
CCTCCAGGTC TGGCCAAGAC TCCTCTGTCA GCACTGGAAC TGAAGCCCCA TGATCACGAC 
TCTGATTCAA ATGAGGGAGA GCAAGATAAC CACACTTCCA GGGACTCAAG AGTTCAACCC 
TTCAACACCT CTTTCTCTTC CACCTACCCT AGTCACAGCA GTGATGGAAG AAGAACCGGT 
TCTTCAGACG TTCACAGCCA CACCCCCTCC TACGCAAACA GACCCACCTC CGCTGAGGGC 
TCCCAGGTCA ACGTCATGGG AGTCCACACA GTGCCCGGCA GTCCCAACAC CCTCCATCGC 
ACCGTAGCCA CCAACACTCC ACCAAGCCCG GCCCTTCAGA GACGTCTGGG TCAAGCCAGT 
CCCTCACTAG CCAGACACCA ACCCCCAGCT GGTGCCAACA GGGACAGTGT CCCCTCCAGT 
CCTCTGATTG GCCGCAACCC TAAAACAGCA GCTGCTGGCG TGCCCCCCAG CCCTCTAATG 
GGCCGCCGAA CGGCGATGAG CGGCCACAGC ACGCCTGATG ACATCGGCCC ACCACCTTCT 
ACGCCTGCCT TCCCTGTGTC CCCACAGCTA CCAGAAAAGA GGCACATGTC CACTGGAGAC 
GTGGAGAGGA CAGATAACAA GAACCTTATG GCCGGTGTTG GCAGCAGTGC GCCAAACCTG 
TCTGGCACGC ACACGTTGAC AGACGTCCAA AAGTCCATCT ATGATGGGTA TCCGGACATT 
AAGATGAATG TGAAGTTTGT CCAAGACACA TCCAAGTTCT GGTACAAGCC AGACATCTCC 
AGAGAGCAAG CCATCAATCT GTTGAAGGAC AGAGAGCCCG GGGCCTTCAT CATAAGGGAC 
AGCCATTCCT TCCGTGGAGC TTACGGCCTT GCAATGAAGG TGGCCTGCCC GCCGCCAACT 
GTCCAGCAGA ACAAAAAAGT TGGAGACTTG ACCAACGAGC TTGTGAGACA CTTCCTGATT 
GAGACAAGCT CCAGAGGTGT TCGACTCAAA GGCTGTCCCA ATGAACCCTA CTTTGGTTGC 
CTCTCAGCGC TGGTTTATCA ACACTCAATG ACCCCGCTAG CTCTGCCCTG TAAGCTGATG 
ATCCCCACCA AAGACCCGAA TGAGGAAGCG CTGGAGTTGG CCACACCCAC TGACCCAGTT 
GTCGAGCTTC TGAAGCAAGG AGCAGTTCAG AAGGTTCCAG AGGAGGCTTA CGCGTGTAAC 
GTCCTCTACA TCAACTCGGT GGACATGGAG TCTCTCACGG GGCCCCAGGC CATCGCCAAG 
GCGATCAGCC TGACTTTGGC GACAAACCCC CTGCCGGCCG CCACCACCGT CCACTTCAAA 
GTGTCTATGC AGGGCATCAC GCTCACCGAC AGCCAGAGGA AACTTTTTTT CAGACGCCAC 
TATCCCATCA ACACCATAAC GTTCTGTGAC ATTGACCCGC AGAACAGGAA ATGGGGAAAA 
GAAGGAGGCG GTTCAGTGAA GCTGTTTGGT TTTGTGGCAC GGAAACAAGG AAGCACAACC 
GACAACGTGA GCCACCTGTT CGCAGAGCTG GACCCAGATC AGCCGGCTTC CGCCATCACC 
AGTTTTGTCT CCAAGACGAT GAAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696059.1
CDS	467..5176
Translation	MEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840199.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840199.1

ORF Insert Sequence:

ATGGAGGAGA ACTATGAGGT AGACCTGGTC TACATCACGG AGAGGATCAT CTCCCTCTGC 
TTCCCCATTG GCATGGAGGA CCAGGGCTAC ATCGCCAACC TCAAGGAGGT GGCCACCATG 
CTGCGATCCA AACATGGAGA TCATTATTTG GTGCTGAACC TGAGTGAATG GAGGACTGAG 
TTATCGAAGC TAAACCACAA GGTCTTGGAA TTTGGCTGGC CGGACTTTCA CGCACCAGCC 
CTGGACAAGA TCTGTAGCAT GTGCAAAGCG ATTGACACGT GGCTCAATGG AGACTCGCAC 
AATGTGGTGG TGCTTCACAA CAAGGGGAAC CGAGGTCGAA CTGGGGTCGT GGTGGCTGCT 
TACATGCACT ATAGCAACAT ATCAGCAAGT GCTGACCAGG CCTTGGACAG GTTCGCCATG 
AGACGGTTCT ACGAGGATAA AGCACTTCCT GTGGGTCAGC CGTCACAAAA AAGGTATGTG 
CGATACTTCA ATGGCCTTCT GTCGGGGCAC ATCAAAATCA ACAACAAACC TCTCTTCCTG 
CACCACGTCA TCATGCACGG TATCCCCAAC TTTGAGTCTA AAGGAGGCTG CCGCCCTTTC 
CTGAAGATCT ACCAAGCGAT GCAGCCAGTC TACACGTCAG GGATATACAA CGTGCAGGGA 
GACAGTAACA CTAGTATCTG CATCACCATC GAACCAGGCC TCCTCCTGAA GGGAGACATC 
CTGCTGAAGT GTTACCACAA ACGATACAGA GGCCCGACCA GAGACGTAGT GTTCCGGGTG 
CAGTTCCACA CCTGTGCCAT CCACGACCTT GGCGTGGTGT TCGGGAAGAG CGAACTGGAC 
GAGACCTTCA AAGACGACAG GTTTCCTGAA TACGGGAAAG TGGAGTTTGT TTTCTCCTAT 
GCACCAGAAA AAATTAGAGG CTTAGACCAC CTGGAGAACG GCCCCAGTGT TTCCGTGGAC 
TACAACACCC AGGAAAACCT GATTCGCTGT GATTCATATG ACAACTTTAG CCAGCGCTCT 
GAAGATGCCG TGGATGGAGA GCGTGAGGTC ATTCACACTC AAGGTCCACT TGATGGAAGC 
CTTTATGCCC GGGTCTGTAA GAAAGACTCC CTGGAGGGAG TTGTCGCCAT CAACGGTAAT 
CCAGTCCATG AAAATTCCTT AGCCAGCACA GAGAACCAGT TACAACACAC AAGCTCCTTG 
CTTCAAACCG CCGAACAGAC CCTTCCTGTG ATTGGCCACG CTTCTCTTCC TGCTGTTGAC 
CACACACTCT CTGTGAGCAG TGACTCGGGC AACTCCACTG CATCCATTAA AACTGATCGT 
ACCGACGAGC ACAGCCAGCC AGTGCACAGC ACTGTCAACC ACAACAATCC GGCAGTTCCC 
CACCCGACAC TTAGTCCCCA GGAGAAAAAA GAGCTTGATC AGCTCCTTAG TGGCCTCGAA 
GCACCGACTC TTCAACGACA AGCCTACCTG GCTACATCCA CCAGTCCGGG AGGCGGTGTC 
CGACACCTGG TCCCAGCACA AGTGCACGTT AACGGAGGCC ACCCAAGGCT AGTTCCTGTC 
CCCTCAACGG AAGAGCGTGA GACGGACATT CTTGACGACG AGCTGCCAAA TAGCCAAGAG 
GGAAACAGTG TGGACAGCCT GGGTACACTG TCATCCTTAG AGGGTCAAGC AACCCCAGGG 
GATCTCTATT ACCAACAAGA GAAGTCCGTC AGCAGACAAA ACGACGGGCC ATATCTTGAG 
AGAGGCGTTT GTCGGGAAAA AAAAAACGAG ATGCCCATGC ATGGAGTTCA TACTCCGATG 
ATGCAGGAAA GACCTGTGGA CTCTGTATCC CCACAAGGGG GATATAACAA ATACCAGAAC 
GGTGGTGGGA TGTACCACTC TGAATCCTAT GGGATTCCAC CTGCCAGCAG CCCGGAGGGA 
AACCCTAAAC TGATGCCCAG GGCCCCAGAG AGAAGCACAA GTAGCCGGGA GGCTGTTCAA 
AGGGGCCTCA ATGCCTGGCA CCAGTATAGC CTCCCAGATG ATCCATTTGG ACCTCCCCTG 
CAGTCAACCC ATAGCATGCC ACAGTTCCCC ACAGCAGCCT CACAGCGGGA CATAGAGCAG 
TCCATCGAGG CACTGAATAT GCTCATGCTT GACCTGGACC CCATTAACTC TCACATGTCC 
AAGTCCCATA GTGCACCCTC CGGGGAGAAC ATCCTGAATT CATCCCAGGC ACCCTTCTCC 
CAGACCCTCT CCAGACCCTC ATACCAAGGA GACTCCGCCA TCCACAGATA CAATAACTCT 
GAATCTGTCA ACAATCCCTC CCATCAGCCC ATATGGTCAG CTGGAAGAGT GTCAGCAGTG 
CCTAACCAGA GCCCTATTAT GGATTCTCAA GCACATTTGC CCCAGAGATC CAGCTCGAGC 
TGTCAACACC AGAAAACCAC CCCAACACAC ACCCCAGAGC CCAATCTCCA TGCACGACAA 
GGAGCCATCC ATTACCCCAT TGATCCCTCC TTTAATTTCA ACCAGCATCT CCCAGTTAGG 
CCTGCAAACA CTTTCCCAGG TGTTTCCCCC TCCCCAGACC TGCAGGGTTC GTCTCCATAC 
CAGGGCTTCA GTGCCTCTTC CTCACCCCTT CCTGCGCTCA CTCCACAGCC AAAGAACACA 
TCGTCTTCAT CCCTGCCTCA AGAACAAGAT GTTGAGGAAG AGGCGCTGAA CTTGGAAGGT 
CTGGTGGCTC ATCGCATTGC CGAGTACAAC GCTCGTATCC GGGGCATCAG CGAAAGCATG 
ACACCGCAAC AATCTGACCG TCACCGCTCC TATTCCTTCT CTGGCGTTCG CTCCAGAGGA 
ATGACCCCAG AAGTGACACA GGACAGCGGT CGACGTAGGA CCACCAGTGA GGGACAGTAC 
CAGAGCAGCC ACGACAGCAC ACCAATGGTT GATTCACCGG ACTTTGCCCA CAGTTTGACC 
CTCAACCCTG GAGGCAGACC CAGAGAGGGC CAGATTCACA GCTACAGGGA GGTGTTGGAG 
GACAGCGAGG TGGACCTTGT CATGCACAGT CCCACCTTCA GAGGCGGTGG TGAGAATTCC 
CCTCAGACCC CCAAGTTCCC TGTGTCACCA CAGACTCCTT ACTTTAACAT GTCTCGTGTC 
CCTCCAGGTC TGGCCAAGAC TCCTCTGTCA GCACTGGAAC TGAAGCCCCA TGATCACGAC 
TCTGATTCAA ATGAGGGAGA GCAAGATAAC CACACTTCCA GGGACTCAAG AGTTCAACCC 
TTCAACACCT CTTTCTCTTC CACCTACCCT AGTCACAGCA GTGATGGAAG AAGAACCGGT 
TCTTCAGACG TTCACAGCCA CACCCCCTCC TACGCAAACA GACCCACCTC CGCTGAGGGC 
TCCCAGGTCA ACGTCATGGG AGTCCACACA GTGCCCGGCA GTCCCAACAC CCTCCATCGC 
ACCGTAGCCA CCAACACTCC ACCAAGCCCG GCCCTTCAGA GACGTCTGGG TCAAGCCAGT 
CCCTCACTAG CCAGACACCA ACCCCCAGCT GGTGCCAACA GGGACAGTGT CCCCTCCAGT 
CCTCTGATTG GCCGCAACCC TAAAACAGCA GCTGCTGGCG TGCCCCCCAG CCCTCTAATG 
GGCCGCCGAA CGGCGATGAG CGGCCACAGC ACGCCTGATG ACATCGGCCC ACCACCTTCT 
ACGCCTGCCT TCCCTGTGTC CCCACAGCTA CCAGAAAAGA GGCACATGTC CACTGGAGAC 
GTGGAGAGGA CAGATAACAA GAACCTTATG GCCGGTGTTG GCAGCAGTGC GCCAAACCTG 
TCTGGCACGC ACACGTTGAC AGACGTCCAA AAGTCCATCT ATGATGGGTA TCCGGACATT 
AAGATGAATG TGAAGTTTGT CCAAGACACA TCCAAGTTCT GGTACAAGCC AGACATCTCC 
AGAGAGCAAG CCATCAATCT GTTGAAGGAC AGAGAGCCCG GGGCCTTCAT CATAAGGGAC 
AGCCATTCCT TCCGTGGAGC TTACGGCCTT GCAATGAAGG TGGCCTGCCC GCCGCCAACT 
GTCCAGCAGA ACAAAAAAGT TGGAGACTTG ACCAACGAGC TTGTGAGACA CTTCCTGATT 
GAGACAAGCT CCAGAGGTGT TCGACTCAAA GGCTGTCCCA ATGAACCCTA CTTTGGTTGC 
CTCTCAGCGC TGGTTTATCA ACACTCAATG ACCCCGCTAG CTCTGCCCTG TAAGCTGATG 
ATCCCCACCA AAGACCCGAA TGAGGAAGCG CTGGAGTTGG CCACACCCAC TGACCCAGTT 
GTCGAGCTTC TGAAGCAAGG AGCAGTTCAG AAGGTTCCAG AGGAGGCTTA CGCGTGTAAC 
GTCCTCTACA TCAACTCGGT GGACATGGAG TCTCTCACGG GGCCCCAGGC CATCGCCAAG 
GCGATCAGCC TGACTTTGGC GACAAACCCC CTGCCGGCCG CCACCACCGT CCACTTCAAA 
GTGTCTATGC AGGGCATCAC GCTCACCGAC AGCCAGAGGA AACTTTTTTT CAGACGCCAC 
TATCCCATCA ACACCATAAC GTTCTGTGAC ATTGACCCGC AGAACAGGAA ATGGGGAAAA 
GAAGGAGGCG GTTCAGTGAA GCTGTTTGGT TTTGTGGCAC GGAAACAAGG AAGCACAACC 
GACAACGTGA GCCACCTGTT CGCAGAGCTG GACCCAGATC AGCCGGCTTC CGCCATCACC 
AGTTTTGTCT CCAAGACGAT GAAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101064247 ( XM_029840201.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696061.1 Takifugu rubripes tensin (LOC101064247), transcript variant X13, mRNA.

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CloneID	OTo71062
Clone ID Related Accession (Same CDS sequence)	XM_029840200.1 , XM_029840199.1 , XM_029840201.1
Accession Version	XM_029840201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	tensin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACTATGAGGT AGACCTGGTC TACATCACGG AGAGGATCAT CTCCCTCTGC 
TTCCCCATTG GCATGGAGGA CCAGGGCTAC ATCGCCAACC TCAAGGAGGT GGCCACCATG 
CTGCGATCCA AACATGGAGA TCATTATTTG GTGCTGAACC TGAGTGAATG GAGGACTGAG 
TTATCGAAGC TAAACCACAA GGTCTTGGAA TTTGGCTGGC CGGACTTTCA CGCACCAGCC 
CTGGACAAGA TCTGTAGCAT GTGCAAAGCG ATTGACACGT GGCTCAATGG AGACTCGCAC 
AATGTGGTGG TGCTTCACAA CAAGGGGAAC CGAGGTCGAA CTGGGGTCGT GGTGGCTGCT 
TACATGCACT ATAGCAACAT ATCAGCAAGT GCTGACCAGG CCTTGGACAG GTTCGCCATG 
AGACGGTTCT ACGAGGATAA AGCACTTCCT GTGGGTCAGC CGTCACAAAA AAGGTATGTG 
CGATACTTCA ATGGCCTTCT GTCGGGGCAC ATCAAAATCA ACAACAAACC TCTCTTCCTG 
CACCACGTCA TCATGCACGG TATCCCCAAC TTTGAGTCTA AAGGAGGCTG CCGCCCTTTC 
CTGAAGATCT ACCAAGCGAT GCAGCCAGTC TACACGTCAG GGATATACAA CGTGCAGGGA 
GACAGTAACA CTAGTATCTG CATCACCATC GAACCAGGCC TCCTCCTGAA GGGAGACATC 
CTGCTGAAGT GTTACCACAA ACGATACAGA GGCCCGACCA GAGACGTAGT GTTCCGGGTG 
CAGTTCCACA CCTGTGCCAT CCACGACCTT GGCGTGGTGT TCGGGAAGAG CGAACTGGAC 
GAGACCTTCA AAGACGACAG GTTTCCTGAA TACGGGAAAG TGGAGTTTGT TTTCTCCTAT 
GCACCAGAAA AAATTAGAGG CTTAGACCAC CTGGAGAACG GCCCCAGTGT TTCCGTGGAC 
TACAACACCC AGGAAAACCT GATTCGCTGT GATTCATATG ACAACTTTAG CCAGCGCTCT 
GAAGATGCCG TGGATGGAGA GCGTGAGGTC ATTCACACTC AAGGTCCACT TGATGGAAGC 
CTTTATGCCC GGGTCTGTAA GAAAGACTCC CTGGAGGGAG TTGTCGCCAT CAACGGTAAT 
CCAGTCCATG AAAATTCCTT AGCCAGCACA GAGAACCAGT TACAACACAC AAGCTCCTTG 
CTTCAAACCG CCGAACAGAC CCTTCCTGTG ATTGGCCACG CTTCTCTTCC TGCTGTTGAC 
CACACACTCT CTGTGAGCAG TGACTCGGGC AACTCCACTG CATCCATTAA AACTGATCGT 
ACCGACGAGC ACAGCCAGCC AGTGCACAGC ACTGTCAACC ACAACAATCC GGCAGTTCCC 
CACCCGACAC TTAGTCCCCA GGAGAAAAAA GAGCTTGATC AGCTCCTTAG TGGCCTCGAA 
GCACCGACTC TTCAACGACA AGCCTACCTG GCTACATCCA CCAGTCCGGG AGGCGGTGTC 
CGACACCTGG TCCCAGCACA AGTGCACGTT AACGGAGGCC ACCCAAGGCT AGTTCCTGTC 
CCCTCAACGG AAGAGCGTGA GACGGACATT CTTGACGACG AGCTGCCAAA TAGCCAAGAG 
GGAAACAGTG TGGACAGCCT GGGTACACTG TCATCCTTAG AGGGTCAAGC AACCCCAGGG 
GATCTCTATT ACCAACAAGA GAAGTCCGTC AGCAGACAAA ACGACGGGCC ATATCTTGAG 
AGAGGCGTTT GTCGGGAAAA AAAAAACGAG ATGCCCATGC ATGGAGTTCA TACTCCGATG 
ATGCAGGAAA GACCTGTGGA CTCTGTATCC CCACAAGGGG GATATAACAA ATACCAGAAC 
GGTGGTGGGA TGTACCACTC TGAATCCTAT GGGATTCCAC CTGCCAGCAG CCCGGAGGGA 
AACCCTAAAC TGATGCCCAG GGCCCCAGAG AGAAGCACAA GTAGCCGGGA GGCTGTTCAA 
AGGGGCCTCA ATGCCTGGCA CCAGTATAGC CTCCCAGATG ATCCATTTGG ACCTCCCCTG 
CAGTCAACCC ATAGCATGCC ACAGTTCCCC ACAGCAGCCT CACAGCGGGA CATAGAGCAG 
TCCATCGAGG CACTGAATAT GCTCATGCTT GACCTGGACC CCATTAACTC TCACATGTCC 
AAGTCCCATA GTGCACCCTC CGGGGAGAAC ATCCTGAATT CATCCCAGGC ACCCTTCTCC 
CAGACCCTCT CCAGACCCTC ATACCAAGGA GACTCCGCCA TCCACAGATA CAATAACTCT 
GAATCTGTCA ACAATCCCTC CCATCAGCCC ATATGGTCAG CTGGAAGAGT GTCAGCAGTG 
CCTAACCAGA GCCCTATTAT GGATTCTCAA GCACATTTGC CCCAGAGATC CAGCTCGAGC 
TGTCAACACC AGAAAACCAC CCCAACACAC ACCCCAGAGC CCAATCTCCA TGCACGACAA 
GGAGCCATCC ATTACCCCAT TGATCCCTCC TTTAATTTCA ACCAGCATCT CCCAGTTAGG 
CCTGCAAACA CTTTCCCAGG TGTTTCCCCC TCCCCAGACC TGCAGGGTTC GTCTCCATAC 
CAGGGCTTCA GTGCCTCTTC CTCACCCCTT CCTGCGCTCA CTCCACAGCC AAAGAACACA 
TCGTCTTCAT CCCTGCCTCA AGAACAAGAT GTTGAGGAAG AGGCGCTGAA CTTGGAAGGT 
CTGGTGGCTC ATCGCATTGC CGAGTACAAC GCTCGTATCC GGGGCATCAG CGAAAGCATG 
ACACCGCAAC AATCTGACCG TCACCGCTCC TATTCCTTCT CTGGCGTTCG CTCCAGAGGA 
ATGACCCCAG AAGTGACACA GGACAGCGGT CGACGTAGGA CCACCAGTGA GGGACAGTAC 
CAGAGCAGCC ACGACAGCAC ACCAATGGTT GATTCACCGG ACTTTGCCCA CAGTTTGACC 
CTCAACCCTG GAGGCAGACC CAGAGAGGGC CAGATTCACA GCTACAGGGA GGTGTTGGAG 
GACAGCGAGG TGGACCTTGT CATGCACAGT CCCACCTTCA GAGGCGGTGG TGAGAATTCC 
CCTCAGACCC CCAAGTTCCC TGTGTCACCA CAGACTCCTT ACTTTAACAT GTCTCGTGTC 
CCTCCAGGTC TGGCCAAGAC TCCTCTGTCA GCACTGGAAC TGAAGCCCCA TGATCACGAC 
TCTGATTCAA ATGAGGGAGA GCAAGATAAC CACACTTCCA GGGACTCAAG AGTTCAACCC 
TTCAACACCT CTTTCTCTTC CACCTACCCT AGTCACAGCA GTGATGGAAG AAGAACCGGT 
TCTTCAGACG TTCACAGCCA CACCCCCTCC TACGCAAACA GACCCACCTC CGCTGAGGGC 
TCCCAGGTCA ACGTCATGGG AGTCCACACA GTGCCCGGCA GTCCCAACAC CCTCCATCGC 
ACCGTAGCCA CCAACACTCC ACCAAGCCCG GCCCTTCAGA GACGTCTGGG TCAAGCCAGT 
CCCTCACTAG CCAGACACCA ACCCCCAGCT GGTGCCAACA GGGACAGTGT CCCCTCCAGT 
CCTCTGATTG GCCGCAACCC TAAAACAGCA GCTGCTGGCG TGCCCCCCAG CCCTCTAATG 
GGCCGCCGAA CGGCGATGAG CGGCCACAGC ACGCCTGATG ACATCGGCCC ACCACCTTCT 
ACGCCTGCCT TCCCTGTGTC CCCACAGCTA CCAGAAAAGA GGCACATGTC CACTGGAGAC 
GTGGAGAGGA CAGATAACAA GAACCTTATG GCCGGTGTTG GCAGCAGTGC GCCAAACCTG 
TCTGGCACGC ACACGTTGAC AGACGTCCAA AAGTCCATCT ATGATGGGTA TCCGGACATT 
AAGATGAATG TGAAGTTTGT CCAAGACACA TCCAAGTTCT GGTACAAGCC AGACATCTCC 
AGAGAGCAAG CCATCAATCT GTTGAAGGAC AGAGAGCCCG GGGCCTTCAT CATAAGGGAC 
AGCCATTCCT TCCGTGGAGC TTACGGCCTT GCAATGAAGG TGGCCTGCCC GCCGCCAACT 
GTCCAGCAGA ACAAAAAAGT TGGAGACTTG ACCAACGAGC TTGTGAGACA CTTCCTGATT 
GAGACAAGCT CCAGAGGTGT TCGACTCAAA GGCTGTCCCA ATGAACCCTA CTTTGGTTGC 
CTCTCAGCGC TGGTTTATCA ACACTCAATG ACCCCGCTAG CTCTGCCCTG TAAGCTGATG 
ATCCCCACCA AAGACCCGAA TGAGGAAGCG CTGGAGTTGG CCACACCCAC TGACCCAGTT 
GTCGAGCTTC TGAAGCAAGG AGCAGTTCAG AAGGTTCCAG AGGAGGCTTA CGCGTGTAAC 
GTCCTCTACA TCAACTCGGT GGACATGGAG TCTCTCACGG GGCCCCAGGC CATCGCCAAG 
GCGATCAGCC TGACTTTGGC GACAAACCCC CTGCCGGCCG CCACCACCGT CCACTTCAAA 
GTGTCTATGC AGGGCATCAC GCTCACCGAC AGCCAGAGGA AACTTTTTTT CAGACGCCAC 
TATCCCATCA ACACCATAAC GTTCTGTGAC ATTGACCCGC AGAACAGGAA ATGGGGAAAA 
GAAGGAGGCG GTTCAGTGAA GCTGTTTGGT TTTGTGGCAC GGAAACAAGG AAGCACAACC 
GACAACGTGA GCCACCTGTT CGCAGAGCTG GACCCAGATC AGCCGGCTTC CGCCATCACC 
AGTTTTGTCT CCAAGACGAT GAAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696061.1
CDS	178..4887
Translation	MEENYEVDLVYITERIISLCFPIGMEDQGYIANLKEVATMLRSKHGDHYLVLNLSEWRTELSKLNHKVLEFGWPDFHAPALDKICSMCKAIDTWLNGDSHNVVVLHNKGNRGRTGVVVAAYMHYSNISASADQALDRFAMRRFYEDKALPVGQPSQKRYVRYFNGLLSGHIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRGPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDDRFPEYGKVEFVFSYAPEKIRGLDHLENGPSVSVDYNTQENLIRCDSYDNFSQRSEDAVDGEREVIHTQGPLDGSLYARVCKKDSLEGVVAINGNPVHENSLASTENQLQHTSSLLQTAEQTLPVIGHASLPAVDHTLSVSSDSGNSTASIKTDRTDEHSQPVHSTVNHNNPAVPHPTLSPQEKKELDQLLSGLEAPTLQRQAYLATSTSPGGGVRHLVPAQVHVNGGHPRLVPVPSTEERETDILDDELPNSQEGNSVDSLGTLSSLEGQATPGDLYYQQEKSVSRQNDGPYLERGVCREKKNEMPMHGVHTPMMQERPVDSVSPQGGYNKYQNGGGMYHSESYGIPPASSPEGNPKLMPRAPERSTSSREAVQRGLNAWHQYSLPDDPFGPPLQSTHSMPQFPTAASQRDIEQSIEALNMLMLDLDPINSHMSKSHSAPSGENILNSSQAPFSQTLSRPSYQGDSAIHRYNNSESVNNPSHQPIWSAGRVSAVPNQSPIMDSQAHLPQRSSSSCQHQKTTPTHTPEPNLHARQGAIHYPIDPSFNFNQHLPVRPANTFPGVSPSPDLQGSSPYQGFSASSSPLPALTPQPKNTSSSSLPQEQDVEEEALNLEGLVAHRIAEYNARIRGISESMTPQQSDRHRSYSFSGVRSRGMTPEVTQDSGRRRTTSEGQYQSSHDSTPMVDSPDFAHSLTLNPGGRPREGQIHSYREVLEDSEVDLVMHSPTFRGGGENSPQTPKFPVSPQTPYFNMSRVPPGLAKTPLSALELKPHDHDSDSNEGEQDNHTSRDSRVQPFNTSFSSTYPSHSSDGRRTGSSDVHSHTPSYANRPTSAEGSQVNVMGVHTVPGSPNTLHRTVATNTPPSPALQRRLGQASPSLARHQPPAGANRDSVPSSPLIGRNPKTAAAGVPPSPLMGRRTAMSGHSTPDDIGPPPSTPAFPVSPQLPEKRHMSTGDVERTDNKNLMAGVGSSAPNLSGTHTLTDVQKSIYDGYPDIKMNVKFVQDTSKFWYKPDISREQAINLLKDREPGAFIIRDSHSFRGAYGLAMKVACPPPTVQQNKKVGDLTNELVRHFLIETSSRGVRLKGCPNEPYFGCLSALVYQHSMTPLALPCKLMIPTKDPNEEALELATPTDPVVELLKQGAVQKVPEEAYACNVLYINSVDMESLTGPQAIAKAISLTLATNPLPAATTVHFKVSMQGITLTDSQRKLFFRRHYPINTITFCDIDPQNRKWGKEGGGSVKLFGFVARKQGSTTDNVSHLFAELDPDQPASAITSFVSKTMKR

Target ORF information:

RefSeq Version	XM_029840201.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes tensin (LOC101064247), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840201.1

ORF Insert Sequence:

ATGGAGGAGA ACTATGAGGT AGACCTGGTC TACATCACGG AGAGGATCAT CTCCCTCTGC 
TTCCCCATTG GCATGGAGGA CCAGGGCTAC ATCGCCAACC TCAAGGAGGT GGCCACCATG 
CTGCGATCCA AACATGGAGA TCATTATTTG GTGCTGAACC TGAGTGAATG GAGGACTGAG 
TTATCGAAGC TAAACCACAA GGTCTTGGAA TTTGGCTGGC CGGACTTTCA CGCACCAGCC 
CTGGACAAGA TCTGTAGCAT GTGCAAAGCG ATTGACACGT GGCTCAATGG AGACTCGCAC 
AATGTGGTGG TGCTTCACAA CAAGGGGAAC CGAGGTCGAA CTGGGGTCGT GGTGGCTGCT 
TACATGCACT ATAGCAACAT ATCAGCAAGT GCTGACCAGG CCTTGGACAG GTTCGCCATG 
AGACGGTTCT ACGAGGATAA AGCACTTCCT GTGGGTCAGC CGTCACAAAA AAGGTATGTG 
CGATACTTCA ATGGCCTTCT GTCGGGGCAC ATCAAAATCA ACAACAAACC TCTCTTCCTG 
CACCACGTCA TCATGCACGG TATCCCCAAC TTTGAGTCTA AAGGAGGCTG CCGCCCTTTC 
CTGAAGATCT ACCAAGCGAT GCAGCCAGTC TACACGTCAG GGATATACAA CGTGCAGGGA 
GACAGTAACA CTAGTATCTG CATCACCATC GAACCAGGCC TCCTCCTGAA GGGAGACATC 
CTGCTGAAGT GTTACCACAA ACGATACAGA GGCCCGACCA GAGACGTAGT GTTCCGGGTG 
CAGTTCCACA CCTGTGCCAT CCACGACCTT GGCGTGGTGT TCGGGAAGAG CGAACTGGAC 
GAGACCTTCA AAGACGACAG GTTTCCTGAA TACGGGAAAG TGGAGTTTGT TTTCTCCTAT 
GCACCAGAAA AAATTAGAGG CTTAGACCAC CTGGAGAACG GCCCCAGTGT TTCCGTGGAC 
TACAACACCC AGGAAAACCT GATTCGCTGT GATTCATATG ACAACTTTAG CCAGCGCTCT 
GAAGATGCCG TGGATGGAGA GCGTGAGGTC ATTCACACTC AAGGTCCACT TGATGGAAGC 
CTTTATGCCC GGGTCTGTAA GAAAGACTCC CTGGAGGGAG TTGTCGCCAT CAACGGTAAT 
CCAGTCCATG AAAATTCCTT AGCCAGCACA GAGAACCAGT TACAACACAC AAGCTCCTTG 
CTTCAAACCG CCGAACAGAC CCTTCCTGTG ATTGGCCACG CTTCTCTTCC TGCTGTTGAC 
CACACACTCT CTGTGAGCAG TGACTCGGGC AACTCCACTG CATCCATTAA AACTGATCGT 
ACCGACGAGC ACAGCCAGCC AGTGCACAGC ACTGTCAACC ACAACAATCC GGCAGTTCCC 
CACCCGACAC TTAGTCCCCA GGAGAAAAAA GAGCTTGATC AGCTCCTTAG TGGCCTCGAA 
GCACCGACTC TTCAACGACA AGCCTACCTG GCTACATCCA CCAGTCCGGG AGGCGGTGTC 
CGACACCTGG TCCCAGCACA AGTGCACGTT AACGGAGGCC ACCCAAGGCT AGTTCCTGTC 
CCCTCAACGG AAGAGCGTGA GACGGACATT CTTGACGACG AGCTGCCAAA TAGCCAAGAG 
GGAAACAGTG TGGACAGCCT GGGTACACTG TCATCCTTAG AGGGTCAAGC AACCCCAGGG 
GATCTCTATT ACCAACAAGA GAAGTCCGTC AGCAGACAAA ACGACGGGCC ATATCTTGAG 
AGAGGCGTTT GTCGGGAAAA AAAAAACGAG ATGCCCATGC ATGGAGTTCA TACTCCGATG 
ATGCAGGAAA GACCTGTGGA CTCTGTATCC CCACAAGGGG GATATAACAA ATACCAGAAC 
GGTGGTGGGA TGTACCACTC TGAATCCTAT GGGATTCCAC CTGCCAGCAG CCCGGAGGGA 
AACCCTAAAC TGATGCCCAG GGCCCCAGAG AGAAGCACAA GTAGCCGGGA GGCTGTTCAA 
AGGGGCCTCA ATGCCTGGCA CCAGTATAGC CTCCCAGATG ATCCATTTGG ACCTCCCCTG 
CAGTCAACCC ATAGCATGCC ACAGTTCCCC ACAGCAGCCT CACAGCGGGA CATAGAGCAG 
TCCATCGAGG CACTGAATAT GCTCATGCTT GACCTGGACC CCATTAACTC TCACATGTCC 
AAGTCCCATA GTGCACCCTC CGGGGAGAAC ATCCTGAATT CATCCCAGGC ACCCTTCTCC 
CAGACCCTCT CCAGACCCTC ATACCAAGGA GACTCCGCCA TCCACAGATA CAATAACTCT 
GAATCTGTCA ACAATCCCTC CCATCAGCCC ATATGGTCAG CTGGAAGAGT GTCAGCAGTG 
CCTAACCAGA GCCCTATTAT GGATTCTCAA GCACATTTGC CCCAGAGATC CAGCTCGAGC 
TGTCAACACC AGAAAACCAC CCCAACACAC ACCCCAGAGC CCAATCTCCA TGCACGACAA 
GGAGCCATCC ATTACCCCAT TGATCCCTCC TTTAATTTCA ACCAGCATCT CCCAGTTAGG 
CCTGCAAACA CTTTCCCAGG TGTTTCCCCC TCCCCAGACC TGCAGGGTTC GTCTCCATAC 
CAGGGCTTCA GTGCCTCTTC CTCACCCCTT CCTGCGCTCA CTCCACAGCC AAAGAACACA 
TCGTCTTCAT CCCTGCCTCA AGAACAAGAT GTTGAGGAAG AGGCGCTGAA CTTGGAAGGT 
CTGGTGGCTC ATCGCATTGC CGAGTACAAC GCTCGTATCC GGGGCATCAG CGAAAGCATG 
ACACCGCAAC AATCTGACCG TCACCGCTCC TATTCCTTCT CTGGCGTTCG CTCCAGAGGA 
ATGACCCCAG AAGTGACACA GGACAGCGGT CGACGTAGGA CCACCAGTGA GGGACAGTAC 
CAGAGCAGCC ACGACAGCAC ACCAATGGTT GATTCACCGG ACTTTGCCCA CAGTTTGACC 
CTCAACCCTG GAGGCAGACC CAGAGAGGGC CAGATTCACA GCTACAGGGA GGTGTTGGAG 
GACAGCGAGG TGGACCTTGT CATGCACAGT CCCACCTTCA GAGGCGGTGG TGAGAATTCC 
CCTCAGACCC CCAAGTTCCC TGTGTCACCA CAGACTCCTT ACTTTAACAT GTCTCGTGTC 
CCTCCAGGTC TGGCCAAGAC TCCTCTGTCA GCACTGGAAC TGAAGCCCCA TGATCACGAC 
TCTGATTCAA ATGAGGGAGA GCAAGATAAC CACACTTCCA GGGACTCAAG AGTTCAACCC 
TTCAACACCT CTTTCTCTTC CACCTACCCT AGTCACAGCA GTGATGGAAG AAGAACCGGT 
TCTTCAGACG TTCACAGCCA CACCCCCTCC TACGCAAACA GACCCACCTC CGCTGAGGGC 
TCCCAGGTCA ACGTCATGGG AGTCCACACA GTGCCCGGCA GTCCCAACAC CCTCCATCGC 
ACCGTAGCCA CCAACACTCC ACCAAGCCCG GCCCTTCAGA GACGTCTGGG TCAAGCCAGT 
CCCTCACTAG CCAGACACCA ACCCCCAGCT GGTGCCAACA GGGACAGTGT CCCCTCCAGT 
CCTCTGATTG GCCGCAACCC TAAAACAGCA GCTGCTGGCG TGCCCCCCAG CCCTCTAATG 
GGCCGCCGAA CGGCGATGAG CGGCCACAGC ACGCCTGATG ACATCGGCCC ACCACCTTCT 
ACGCCTGCCT TCCCTGTGTC CCCACAGCTA CCAGAAAAGA GGCACATGTC CACTGGAGAC 
GTGGAGAGGA CAGATAACAA GAACCTTATG GCCGGTGTTG GCAGCAGTGC GCCAAACCTG 
TCTGGCACGC ACACGTTGAC AGACGTCCAA AAGTCCATCT ATGATGGGTA TCCGGACATT 
AAGATGAATG TGAAGTTTGT CCAAGACACA TCCAAGTTCT GGTACAAGCC AGACATCTCC 
AGAGAGCAAG CCATCAATCT GTTGAAGGAC AGAGAGCCCG GGGCCTTCAT CATAAGGGAC 
AGCCATTCCT TCCGTGGAGC TTACGGCCTT GCAATGAAGG TGGCCTGCCC GCCGCCAACT 
GTCCAGCAGA ACAAAAAAGT TGGAGACTTG ACCAACGAGC TTGTGAGACA CTTCCTGATT 
GAGACAAGCT CCAGAGGTGT TCGACTCAAA GGCTGTCCCA ATGAACCCTA CTTTGGTTGC 
CTCTCAGCGC TGGTTTATCA ACACTCAATG ACCCCGCTAG CTCTGCCCTG TAAGCTGATG 
ATCCCCACCA AAGACCCGAA TGAGGAAGCG CTGGAGTTGG CCACACCCAC TGACCCAGTT 
GTCGAGCTTC TGAAGCAAGG AGCAGTTCAG AAGGTTCCAG AGGAGGCTTA CGCGTGTAAC 
GTCCTCTACA TCAACTCGGT GGACATGGAG TCTCTCACGG GGCCCCAGGC CATCGCCAAG 
GCGATCAGCC TGACTTTGGC GACAAACCCC CTGCCGGCCG CCACCACCGT CCACTTCAAA 
GTGTCTATGC AGGGCATCAC GCTCACCGAC AGCCAGAGGA AACTTTTTTT CAGACGCCAC 
TATCCCATCA ACACCATAAC GTTCTGTGAC ATTGACCCGC AGAACAGGAA ATGGGGAAAA 
GAAGGAGGCG GTTCAGTGAA GCTGTTTGGT TTTGTGGCAC GGAAACAAGG AAGCACAACC 
GACAACGTGA GCCACCTGTT CGCAGAGCTG GACCCAGATC AGCCGGCTTC CGCCATCACC 
AGTTTTGTCT CCAAGACGAT GAAGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Gene Symbol	LOC101064247
Entrez Gene ID	101064247
Full Name	tensin 1
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

LOC101064247 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

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