MDC1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	MDC1
Entrez Gene ID	103092449
Full Name	mediator of DNA damage checkpoint 1
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016430938.1	XP_016286424.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_016430939.1	XP_016286425.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_007483500.2	XP_007483562.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_007483501.2	XP_007483563.1	mediator of DNA damage checkpoint protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following MDC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc11773	XM_016430939.1 Latest version!	Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMc11773	XM_016430938.1 Latest version!	Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMc11773	XM_007483500.2 Latest version!	Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMc11774	XM_007483501.2 Latest version!	Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDC1 ( XM_016430938.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016286424.1 Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMc11773
Clone ID Related Accession (Same CDS sequence)	XM_016430938.1 , XM_016430939.1 , XM_007483500.2
Accession Version	XM_016430938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CTCAATTGGT GGACTGGGAG GCTCAGGAGG AGGAGCCTGA GGATTCTAAT 
GGCTCTCCTC CACAATTCGG CTTGGAGCCT GTAGGGCGGC TGCACCTGTT CAGCAGTGTT 
CGAGGTCCTG AGAAAGATTT CCTGTTGTAC CCAGGGGAGA ATGTGGTTGG CCGAATACCA 
GGCTGCTCAG TTGCTTTGCC TTTTCCATCC ATTTCCAAAC ACCATGCAAT AATTGAGATT 
TCAGCCCAAG GCAGGGCACC TATCCTTCGA GATTGTGGGA GCCTCAATCA TACTCGCCTT 
CTAAGACCTC CCAAGCTCCT AAGCCCTGGG GTGGGCCATC AGTTGAGAGA CCAAGATTTG 
GTACTCTTTG CTGACCTGCC TTGCCAGTAT CACCGCCTGG GTACCCCCCA ACTCTCTGGC 
CGACGGGGGG GCCTAAGTGT GGAAGAGACA CCCAGGGTTC CAGGAATAGG AACAGCCCGG 
TTCCAGGGGA CCTTATTGGC TGAAGATTCA GAGGAGGAAG GAGATTCTCC TTTGGATAGG 
AGTGTGGAGG TACCCATCAC ATACTCCCCC TCTGAAACTA TTGTTCCAGA GAGTGATGTG 
GAGGGAGCTT CACCAGGCAC CAAGGATTCT GCCCTGCCTC TTACCTTCAT CTTGGATAGT 
GATACAGATG AAGAAGATTC TCCTCCAGTG GAGTCCTCCT CAGCTACCAG GAGAGGCTCC 
ACTGTGGATA TGGAGTGGAA TAGGACTGAT GGAGAAAACC ATTCCTCAGC TGTGGGGAGG 
GACAGTGATA CAGATGTTGA TGAAGAAGGC AGTCTCCAAA GGACCTTAGT TGTGGCACCT 
TTGGCAGGGA CTCAGCCTTC TTCTCTTGAA GATAGTAGTA CAGATGTGGA AGAGGAGGGG 
CTTTCAGTGA GACCAGTTGT AGCACAAACT GATATAGGGG ACAGTGATAC AGATATAGAA 
GAGGACTCTG TCCCAGCAGC GCCACCTGTA GTCCATCTAG GGAAAGTTCA GGGACCCATG 
GGTGGGACAA ACAATACAGA TATAAAACAG GAGGGGGCCC CAACAGAACT CTCCATAGTG 
CATCTTGAAA AGGACCAGCA CCCTACTGGT GGGGATAGGG GGGACAGTGA CACAGATGCA 
GATGACAATG GATCTCTGAC ATATATGATA AAGAGCCAAC TTCCACCTGA AGAAGGCAGT 
AGGGTAGAGT GGGCCCCAGC CACAGCTCAA GGGGAAAAGA CCACTAAGAA ATTTGGGGCC 
CAGGGTAGGA TCCCTATAAC AGAATTCGAG CAGGGTCTAT CTCCATTCCC AAGGGTCAGC 
TCAGTAGGCA CATTGGTAGA ACCAAGCATT CAAGGGAAGT CAGCTCAGGT ACTTTTGGAA 
GGAACCCAGA ATTATGTTGG AGGTAGTGAT CCAGATGTGG AAGGTACTGA AGACTTTCAG 
AACAAGAGTG ATGACAATTC AGACCTGGAT TTACAAGATA CTCAGTGCTT TGTAGAAGGA 
GGAAATCAGA ACCATGGAGA TGGTCCAGAT GAACCATGGG AAATTTTGGC CACACAGCCA 
TTCTGCTCTG AGCGGTCAGA GGCTTCAGAA ACTCAGCCCA TCAAATCTTA CCTTGCAGCT 
CATGGAGCAT GTCTCTCCCC ACCTAGTACA ACCTCAGGGG ACCTACAGCC AGGGAGTTCA 
GACCAAACAG AGCCCTGGGG GAATCAAGGC ACAGAGAATC CAAAAACAGA GATCCAGGGA 
CAAACTATGG AGAGTGGCAT GGAGAACCAG GGGATAGAAA CAGAGATACC TAGAGTAATA 
CCAGAGAGAA AAATGGAAAA AGGGCCCTTT AAGAGAAAGA CATTGGCAAA TAAGACAAAG 
AGATCCAAAA GAAAAAGAGT GAGTCCAGTG GTACAAACAG AGATACCAGA GAAAGGACAG 
AGACGTACAG CAGGTAGGTC GAAGAATGTT ATGATAGAGA CAGACAACAA AAGCTGTATA 
GAAACAAAGG AAATGGAGAG GCAAATCCCT GAGAGAGACA CATTTGAGGG ACAAATACCA 
GGAAAAGAAA CCAAGAATCT GGTAGTAGAA GTGAAGGTGC CCAGAGAAAT GCTGGAGAGA 
GGGGCAGAGG GACAAATGGC AGAAAGTTAT GACCAGAAGG CCCAGAATTC AACTCCAGTA 
CCAAAATCTA AAACTGGGGA AGGAGACATG TCGGAATTAG TCTCAAACCC CCAAATCCTC 
AATGATGGCA GACATCAGAG AGGTCTTGCT GACAACAAAA CTCTGCCTCC TGAGGAAGTG 
CCAATGGATG ACCAGGACTA CTCAAATGTA CCTTTTGTAG CATCTAAAGT TCCAGTTCCT 
GAAGCCCCAA CTCCTACTTC AACTCCTAGA ATCACTCGGA GTCAAAACAG GAGAGGATCA 
AAACTCCTCC ATTCTTCTTC CTCTCTAGCA TCTCAGGAGT CACCTATATT AGGAGCCACA 
CGAAGCAGGA GGCAGGGAAC CCAGGGGATT CTCCCAAAAA TTGAGTCTCT CCCCTCTCAG 
GATTCTAGAA ACAAATCAGA GCTTTCTCTC CAGAGCCAGA CACAGAAGTC TTTCAGTACC 
TCCTCTGGAC CAACTGCACC CTTGGCTCCT GAACTTCACA CTCCCATCCC TAAAGAATTG 
CCTTTAATCC CTCAACCTCA CCTTCAGACT CGTCAAAACC GGAAACGGGG GACCACCAGA 
ACTTGTGGGT CTACTGCAGT TTCACCAGTC CTCCCTGAGC ACCCCCCTCC CATTTCAACA 
GACAGATCTG AAACTCTTGT TCCCCAACCC AGAGCACTCC GAAGTCAGAG GGCAAGAGTA 
GGAGAAACTT CTGAGTCACT CACGGCCACC CCAGAGGCCT CCTATTCTCT GACACCTGAG 
GCACCTGCTC CTCCAGCTAC CAGGAGTCGA AGAACAGGGG CATCAGGAAT GCCTGGTTCT 
ACTCCAGAGA CCCAGCCTAA GCTGCTTCCA GGCAGAAAGC GGCCTCTAGC CACCAAGGAA 
TCATCTCCAT GCCCAAAGCA ACCCCGGAGA GGACGGTCCC AAAGACAAGA ATCGAGCAAG 
GAAGATGAAG AGAAGCCAAT GGAAGTTGAG ATTGCTTCGA AGACCCCAGT GGGGGTTGCA 
GAACCAGCAG TTCAAGCAAA GGAGGAAGTC AAGGGGGCAC CTAGCCGAAG CTTGCGTCGA 
GCTAGACTCA GTAGAGAACC TCGAGCTCCC AAGGTTCTCT TCACAGGTGT GGTGGATGAT 
CGAGGAGAGC AGGCTGTGCT GGCACTGGGT GGAAGTCTGG CCAGTTCAGT GGCAGAAGCC 
TCACACTTGG TGACAGATCG AGTTAGAAGG ACAGTCAAAT TCCTATGTGC CTTGGGGCGG 
GGGATACCAA TTCTGTCTTT GGAATGGTTG CACCAGTCTA GAAAGGTTGG CCGATTCCTG 
CCACCTGAGG AATTTGTGGT GAATGATCCA GAGCAGGAGA AGAACTTTGG CTTCAGCCTT 
CGAGAGGCAC TAAGCCGGGC CCAGGAGAGG GGCCTACTGG AGGGCTATGA AATCTTTGTA 
ACTCCTGGAG TGCAGCCCCC ACCCCCCCAA ATGGGAGAGA TCATTACCTG CTGTGGAGGC 
TCTGCACTTT CTAGCATGCC CCGGGCCTAT AAGCCCCAGA GACTGGTGAT TTCGTGTCCC 
CAGGACCTCT CCAGATGCTC TGCTGCTGCT AGAGCTAAGC TGCCCCTCCT TTCCCCAGAG 
TTCCTGCTCA CTGGGGTGCT GAGGCAGGAA GTCCAGCTGG AAGCCTTCCT CCTTTCCACC 
CCAGACCCTG TACCTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016286424.1
CDS	570..4370
Translation	MEDTQLVDWEAQEEEPEDSNGSPPQFGLEPVGRLHLFSSVRGPEKDFLLYPGENVVGRIPGCSVALPFPSISKHHAIIEISAQGRAPILRDCGSLNHTRLLRPPKLLSPGVGHQLRDQDLVLFADLPCQYHRLGTPQLSGRRGGLSVEETPRVPGIGTARFQGTLLAEDSEEEGDSPLDRSVEVPITYSPSETIVPESDVEGASPGTKDSALPLTFILDSDTDEEDSPPVESSSATRRGSTVDMEWNRTDGENHSSAVGRDSDTDVDEEGSLQRTLVVAPLAGTQPSSLEDSSTDVEEEGLSVRPVVAQTDIGDSDTDIEEDSVPAAPPVVHLGKVQGPMGGTNNTDIKQEGAPTELSIVHLEKDQHPTGGDRGDSDTDADDNGSLTYMIKSQLPPEEGSRVEWAPATAQGEKTTKKFGAQGRIPITEFEQGLSPFPRVSSVGTLVEPSIQGKSAQVLLEGTQNYVGGSDPDVEGTEDFQNKSDDNSDLDLQDTQCFVEGGNQNHGDGPDEPWEILATQPFCSERSEASETQPIKSYLAAHGACLSPPSTTSGDLQPGSSDQTEPWGNQGTENPKTEIQGQTMESGMENQGIETEIPRVIPERKMEKGPFKRKTLANKTKRSKRKRVSPVVQTEIPEKGQRRTAGRSKNVMIETDNKSCIETKEMERQIPERDTFEGQIPGKETKNLVVEVKVPREMLERGAEGQMAESYDQKAQNSTPVPKSKTGEGDMSELVSNPQILNDGRHQRGLADNKTLPPEEVPMDDQDYSNVPFVASKVPVPEAPTPTSTPRITRSQNRRGSKLLHSSSSLASQESPILGATRSRRQGTQGILPKIESLPSQDSRNKSELSLQSQTQKSFSTSSGPTAPLAPELHTPIPKELPLIPQPHLQTRQNRKRGTTRTCGSTAVSPVLPEHPPPISTDRSETLVPQPRALRSQRARVGETSESLTATPEASYSLTPEAPAPPATRSRRTGASGMPGSTPETQPKLLPGRKRPLATKESSPCPKQPRRGRSQRQESSKEDEEKPMEVEIASKTPVGVAEPAVQAKEEVKGAPSRSLRRARLSREPRAPKVLFTGVVDDRGEQAVLALGGSLASSVAEASHLVTDRVRRTVKFLCALGRGIPILSLEWLHQSRKVGRFLPPEEFVVNDPEQEKNFGFSLREALSRAQERGLLEGYEIFVTPGVQPPPPQMGEIITCCGGSALSSMPRAYKPQRLVISCPQDLSRCSAAARAKLPLLSPEFLLTGVLRQEVQLEAFLLSTPDPVPS

Target ORF information:

RefSeq Version	XM_016430938.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016430938.1

ORF Insert Sequence:

ATGGAGGACA CTCAATTGGT GGACTGGGAG GCTCAGGAGG AGGAGCCTGA GGATTCTAAT 
GGCTCTCCTC CACAATTCGG CTTGGAGCCT GTAGGGCGGC TGCACCTGTT CAGCAGTGTT 
CGAGGTCCTG AGAAAGATTT CCTGTTGTAC CCAGGGGAGA ATGTGGTTGG CCGAATACCA 
GGCTGCTCAG TTGCTTTGCC TTTTCCATCC ATTTCCAAAC ACCATGCAAT AATTGAGATT 
TCAGCCCAAG GCAGGGCACC TATCCTTCGA GATTGTGGGA GCCTCAATCA TACTCGCCTT 
CTAAGACCTC CCAAGCTCCT AAGCCCTGGG GTGGGCCATC AGTTGAGAGA CCAAGATTTG 
GTACTCTTTG CTGACCTGCC TTGCCAGTAT CACCGCCTGG GTACCCCCCA ACTCTCTGGC 
CGACGGGGGG GCCTAAGTGT GGAAGAGACA CCCAGGGTTC CAGGAATAGG AACAGCCCGG 
TTCCAGGGGA CCTTATTGGC TGAAGATTCA GAGGAGGAAG GAGATTCTCC TTTGGATAGG 
AGTGTGGAGG TACCCATCAC ATACTCCCCC TCTGAAACTA TTGTTCCAGA GAGTGATGTG 
GAGGGAGCTT CACCAGGCAC CAAGGATTCT GCCCTGCCTC TTACCTTCAT CTTGGATAGT 
GATACAGATG AAGAAGATTC TCCTCCAGTG GAGTCCTCCT CAGCTACCAG GAGAGGCTCC 
ACTGTGGATA TGGAGTGGAA TAGGACTGAT GGAGAAAACC ATTCCTCAGC TGTGGGGAGG 
GACAGTGATA CAGATGTTGA TGAAGAAGGC AGTCTCCAAA GGACCTTAGT TGTGGCACCT 
TTGGCAGGGA CTCAGCCTTC TTCTCTTGAA GATAGTAGTA CAGATGTGGA AGAGGAGGGG 
CTTTCAGTGA GACCAGTTGT AGCACAAACT GATATAGGGG ACAGTGATAC AGATATAGAA 
GAGGACTCTG TCCCAGCAGC GCCACCTGTA GTCCATCTAG GGAAAGTTCA GGGACCCATG 
GGTGGGACAA ACAATACAGA TATAAAACAG GAGGGGGCCC CAACAGAACT CTCCATAGTG 
CATCTTGAAA AGGACCAGCA CCCTACTGGT GGGGATAGGG GGGACAGTGA CACAGATGCA 
GATGACAATG GATCTCTGAC ATATATGATA AAGAGCCAAC TTCCACCTGA AGAAGGCAGT 
AGGGTAGAGT GGGCCCCAGC CACAGCTCAA GGGGAAAAGA CCACTAAGAA ATTTGGGGCC 
CAGGGTAGGA TCCCTATAAC AGAATTCGAG CAGGGTCTAT CTCCATTCCC AAGGGTCAGC 
TCAGTAGGCA CATTGGTAGA ACCAAGCATT CAAGGGAAGT CAGCTCAGGT ACTTTTGGAA 
GGAACCCAGA ATTATGTTGG AGGTAGTGAT CCAGATGTGG AAGGTACTGA AGACTTTCAG 
AACAAGAGTG ATGACAATTC AGACCTGGAT TTACAAGATA CTCAGTGCTT TGTAGAAGGA 
GGAAATCAGA ACCATGGAGA TGGTCCAGAT GAACCATGGG AAATTTTGGC CACACAGCCA 
TTCTGCTCTG AGCGGTCAGA GGCTTCAGAA ACTCAGCCCA TCAAATCTTA CCTTGCAGCT 
CATGGAGCAT GTCTCTCCCC ACCTAGTACA ACCTCAGGGG ACCTACAGCC AGGGAGTTCA 
GACCAAACAG AGCCCTGGGG GAATCAAGGC ACAGAGAATC CAAAAACAGA GATCCAGGGA 
CAAACTATGG AGAGTGGCAT GGAGAACCAG GGGATAGAAA CAGAGATACC TAGAGTAATA 
CCAGAGAGAA AAATGGAAAA AGGGCCCTTT AAGAGAAAGA CATTGGCAAA TAAGACAAAG 
AGATCCAAAA GAAAAAGAGT GAGTCCAGTG GTACAAACAG AGATACCAGA GAAAGGACAG 
AGACGTACAG CAGGTAGGTC GAAGAATGTT ATGATAGAGA CAGACAACAA AAGCTGTATA 
GAAACAAAGG AAATGGAGAG GCAAATCCCT GAGAGAGACA CATTTGAGGG ACAAATACCA 
GGAAAAGAAA CCAAGAATCT GGTAGTAGAA GTGAAGGTGC CCAGAGAAAT GCTGGAGAGA 
GGGGCAGAGG GACAAATGGC AGAAAGTTAT GACCAGAAGG CCCAGAATTC AACTCCAGTA 
CCAAAATCTA AAACTGGGGA AGGAGACATG TCGGAATTAG TCTCAAACCC CCAAATCCTC 
AATGATGGCA GACATCAGAG AGGTCTTGCT GACAACAAAA CTCTGCCTCC TGAGGAAGTG 
CCAATGGATG ACCAGGACTA CTCAAATGTA CCTTTTGTAG CATCTAAAGT TCCAGTTCCT 
GAAGCCCCAA CTCCTACTTC AACTCCTAGA ATCACTCGGA GTCAAAACAG GAGAGGATCA 
AAACTCCTCC ATTCTTCTTC CTCTCTAGCA TCTCAGGAGT CACCTATATT AGGAGCCACA 
CGAAGCAGGA GGCAGGGAAC CCAGGGGATT CTCCCAAAAA TTGAGTCTCT CCCCTCTCAG 
GATTCTAGAA ACAAATCAGA GCTTTCTCTC CAGAGCCAGA CACAGAAGTC TTTCAGTACC 
TCCTCTGGAC CAACTGCACC CTTGGCTCCT GAACTTCACA CTCCCATCCC TAAAGAATTG 
CCTTTAATCC CTCAACCTCA CCTTCAGACT CGTCAAAACC GGAAACGGGG GACCACCAGA 
ACTTGTGGGT CTACTGCAGT TTCACCAGTC CTCCCTGAGC ACCCCCCTCC CATTTCAACA 
GACAGATCTG AAACTCTTGT TCCCCAACCC AGAGCACTCC GAAGTCAGAG GGCAAGAGTA 
GGAGAAACTT CTGAGTCACT CACGGCCACC CCAGAGGCCT CCTATTCTCT GACACCTGAG 
GCACCTGCTC CTCCAGCTAC CAGGAGTCGA AGAACAGGGG CATCAGGAAT GCCTGGTTCT 
ACTCCAGAGA CCCAGCCTAA GCTGCTTCCA GGCAGAAAGC GGCCTCTAGC CACCAAGGAA 
TCATCTCCAT GCCCAAAGCA ACCCCGGAGA GGACGGTCCC AAAGACAAGA ATCGAGCAAG 
GAAGATGAAG AGAAGCCAAT GGAAGTTGAG ATTGCTTCGA AGACCCCAGT GGGGGTTGCA 
GAACCAGCAG TTCAAGCAAA GGAGGAAGTC AAGGGGGCAC CTAGCCGAAG CTTGCGTCGA 
GCTAGACTCA GTAGAGAACC TCGAGCTCCC AAGGTTCTCT TCACAGGTGT GGTGGATGAT 
CGAGGAGAGC AGGCTGTGCT GGCACTGGGT GGAAGTCTGG CCAGTTCAGT GGCAGAAGCC 
TCACACTTGG TGACAGATCG AGTTAGAAGG ACAGTCAAAT TCCTATGTGC CTTGGGGCGG 
GGGATACCAA TTCTGTCTTT GGAATGGTTG CACCAGTCTA GAAAGGTTGG CCGATTCCTG 
CCACCTGAGG AATTTGTGGT GAATGATCCA GAGCAGGAGA AGAACTTTGG CTTCAGCCTT 
CGAGAGGCAC TAAGCCGGGC CCAGGAGAGG GGCCTACTGG AGGGCTATGA AATCTTTGTA 
ACTCCTGGAG TGCAGCCCCC ACCCCCCCAA ATGGGAGAGA TCATTACCTG CTGTGGAGGC 
TCTGCACTTT CTAGCATGCC CCGGGCCTAT AAGCCCCAGA GACTGGTGAT TTCGTGTCCC 
CAGGACCTCT CCAGATGCTC TGCTGCTGCT AGAGCTAAGC TGCCCCTCCT TTCCCCAGAG 
TTCCTGCTCA CTGGGGTGCT GAGGCAGGAA GTCCAGCTGG AAGCCTTCCT CCTTTCCACC 
CCAGACCCTG TACCTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_016430939.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016286425.1 Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMc11773
Clone ID Related Accession (Same CDS sequence)	XM_016430938.1 , XM_016430939.1 , XM_007483500.2
Accession Version	XM_016430939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CTCAATTGGT GGACTGGGAG GCTCAGGAGG AGGAGCCTGA GGATTCTAAT 
GGCTCTCCTC CACAATTCGG CTTGGAGCCT GTAGGGCGGC TGCACCTGTT CAGCAGTGTT 
CGAGGTCCTG AGAAAGATTT CCTGTTGTAC CCAGGGGAGA ATGTGGTTGG CCGAATACCA 
GGCTGCTCAG TTGCTTTGCC TTTTCCATCC ATTTCCAAAC ACCATGCAAT AATTGAGATT 
TCAGCCCAAG GCAGGGCACC TATCCTTCGA GATTGTGGGA GCCTCAATCA TACTCGCCTT 
CTAAGACCTC CCAAGCTCCT AAGCCCTGGG GTGGGCCATC AGTTGAGAGA CCAAGATTTG 
GTACTCTTTG CTGACCTGCC TTGCCAGTAT CACCGCCTGG GTACCCCCCA ACTCTCTGGC 
CGACGGGGGG GCCTAAGTGT GGAAGAGACA CCCAGGGTTC CAGGAATAGG AACAGCCCGG 
TTCCAGGGGA CCTTATTGGC TGAAGATTCA GAGGAGGAAG GAGATTCTCC TTTGGATAGG 
AGTGTGGAGG TACCCATCAC ATACTCCCCC TCTGAAACTA TTGTTCCAGA GAGTGATGTG 
GAGGGAGCTT CACCAGGCAC CAAGGATTCT GCCCTGCCTC TTACCTTCAT CTTGGATAGT 
GATACAGATG AAGAAGATTC TCCTCCAGTG GAGTCCTCCT CAGCTACCAG GAGAGGCTCC 
ACTGTGGATA TGGAGTGGAA TAGGACTGAT GGAGAAAACC ATTCCTCAGC TGTGGGGAGG 
GACAGTGATA CAGATGTTGA TGAAGAAGGC AGTCTCCAAA GGACCTTAGT TGTGGCACCT 
TTGGCAGGGA CTCAGCCTTC TTCTCTTGAA GATAGTAGTA CAGATGTGGA AGAGGAGGGG 
CTTTCAGTGA GACCAGTTGT AGCACAAACT GATATAGGGG ACAGTGATAC AGATATAGAA 
GAGGACTCTG TCCCAGCAGC GCCACCTGTA GTCCATCTAG GGAAAGTTCA GGGACCCATG 
GGTGGGACAA ACAATACAGA TATAAAACAG GAGGGGGCCC CAACAGAACT CTCCATAGTG 
CATCTTGAAA AGGACCAGCA CCCTACTGGT GGGGATAGGG GGGACAGTGA CACAGATGCA 
GATGACAATG GATCTCTGAC ATATATGATA AAGAGCCAAC TTCCACCTGA AGAAGGCAGT 
AGGGTAGAGT GGGCCCCAGC CACAGCTCAA GGGGAAAAGA CCACTAAGAA ATTTGGGGCC 
CAGGGTAGGA TCCCTATAAC AGAATTCGAG CAGGGTCTAT CTCCATTCCC AAGGGTCAGC 
TCAGTAGGCA CATTGGTAGA ACCAAGCATT CAAGGGAAGT CAGCTCAGGT ACTTTTGGAA 
GGAACCCAGA ATTATGTTGG AGGTAGTGAT CCAGATGTGG AAGGTACTGA AGACTTTCAG 
AACAAGAGTG ATGACAATTC AGACCTGGAT TTACAAGATA CTCAGTGCTT TGTAGAAGGA 
GGAAATCAGA ACCATGGAGA TGGTCCAGAT GAACCATGGG AAATTTTGGC CACACAGCCA 
TTCTGCTCTG AGCGGTCAGA GGCTTCAGAA ACTCAGCCCA TCAAATCTTA CCTTGCAGCT 
CATGGAGCAT GTCTCTCCCC ACCTAGTACA ACCTCAGGGG ACCTACAGCC AGGGAGTTCA 
GACCAAACAG AGCCCTGGGG GAATCAAGGC ACAGAGAATC CAAAAACAGA GATCCAGGGA 
CAAACTATGG AGAGTGGCAT GGAGAACCAG GGGATAGAAA CAGAGATACC TAGAGTAATA 
CCAGAGAGAA AAATGGAAAA AGGGCCCTTT AAGAGAAAGA CATTGGCAAA TAAGACAAAG 
AGATCCAAAA GAAAAAGAGT GAGTCCAGTG GTACAAACAG AGATACCAGA GAAAGGACAG 
AGACGTACAG CAGGTAGGTC GAAGAATGTT ATGATAGAGA CAGACAACAA AAGCTGTATA 
GAAACAAAGG AAATGGAGAG GCAAATCCCT GAGAGAGACA CATTTGAGGG ACAAATACCA 
GGAAAAGAAA CCAAGAATCT GGTAGTAGAA GTGAAGGTGC CCAGAGAAAT GCTGGAGAGA 
GGGGCAGAGG GACAAATGGC AGAAAGTTAT GACCAGAAGG CCCAGAATTC AACTCCAGTA 
CCAAAATCTA AAACTGGGGA AGGAGACATG TCGGAATTAG TCTCAAACCC CCAAATCCTC 
AATGATGGCA GACATCAGAG AGGTCTTGCT GACAACAAAA CTCTGCCTCC TGAGGAAGTG 
CCAATGGATG ACCAGGACTA CTCAAATGTA CCTTTTGTAG CATCTAAAGT TCCAGTTCCT 
GAAGCCCCAA CTCCTACTTC AACTCCTAGA ATCACTCGGA GTCAAAACAG GAGAGGATCA 
AAACTCCTCC ATTCTTCTTC CTCTCTAGCA TCTCAGGAGT CACCTATATT AGGAGCCACA 
CGAAGCAGGA GGCAGGGAAC CCAGGGGATT CTCCCAAAAA TTGAGTCTCT CCCCTCTCAG 
GATTCTAGAA ACAAATCAGA GCTTTCTCTC CAGAGCCAGA CACAGAAGTC TTTCAGTACC 
TCCTCTGGAC CAACTGCACC CTTGGCTCCT GAACTTCACA CTCCCATCCC TAAAGAATTG 
CCTTTAATCC CTCAACCTCA CCTTCAGACT CGTCAAAACC GGAAACGGGG GACCACCAGA 
ACTTGTGGGT CTACTGCAGT TTCACCAGTC CTCCCTGAGC ACCCCCCTCC CATTTCAACA 
GACAGATCTG AAACTCTTGT TCCCCAACCC AGAGCACTCC GAAGTCAGAG GGCAAGAGTA 
GGAGAAACTT CTGAGTCACT CACGGCCACC CCAGAGGCCT CCTATTCTCT GACACCTGAG 
GCACCTGCTC CTCCAGCTAC CAGGAGTCGA AGAACAGGGG CATCAGGAAT GCCTGGTTCT 
ACTCCAGAGA CCCAGCCTAA GCTGCTTCCA GGCAGAAAGC GGCCTCTAGC CACCAAGGAA 
TCATCTCCAT GCCCAAAGCA ACCCCGGAGA GGACGGTCCC AAAGACAAGA ATCGAGCAAG 
GAAGATGAAG AGAAGCCAAT GGAAGTTGAG ATTGCTTCGA AGACCCCAGT GGGGGTTGCA 
GAACCAGCAG TTCAAGCAAA GGAGGAAGTC AAGGGGGCAC CTAGCCGAAG CTTGCGTCGA 
GCTAGACTCA GTAGAGAACC TCGAGCTCCC AAGGTTCTCT TCACAGGTGT GGTGGATGAT 
CGAGGAGAGC AGGCTGTGCT GGCACTGGGT GGAAGTCTGG CCAGTTCAGT GGCAGAAGCC 
TCACACTTGG TGACAGATCG AGTTAGAAGG ACAGTCAAAT TCCTATGTGC CTTGGGGCGG 
GGGATACCAA TTCTGTCTTT GGAATGGTTG CACCAGTCTA GAAAGGTTGG CCGATTCCTG 
CCACCTGAGG AATTTGTGGT GAATGATCCA GAGCAGGAGA AGAACTTTGG CTTCAGCCTT 
CGAGAGGCAC TAAGCCGGGC CCAGGAGAGG GGCCTACTGG AGGGCTATGA AATCTTTGTA 
ACTCCTGGAG TGCAGCCCCC ACCCCCCCAA ATGGGAGAGA TCATTACCTG CTGTGGAGGC 
TCTGCACTTT CTAGCATGCC CCGGGCCTAT AAGCCCCAGA GACTGGTGAT TTCGTGTCCC 
CAGGACCTCT CCAGATGCTC TGCTGCTGCT AGAGCTAAGC TGCCCCTCCT TTCCCCAGAG 
TTCCTGCTCA CTGGGGTGCT GAGGCAGGAA GTCCAGCTGG AAGCCTTCCT CCTTTCCACC 
CCAGACCCTG TACCTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016286425.1
CDS	330..4130
Translation	MEDTQLVDWEAQEEEPEDSNGSPPQFGLEPVGRLHLFSSVRGPEKDFLLYPGENVVGRIPGCSVALPFPSISKHHAIIEISAQGRAPILRDCGSLNHTRLLRPPKLLSPGVGHQLRDQDLVLFADLPCQYHRLGTPQLSGRRGGLSVEETPRVPGIGTARFQGTLLAEDSEEEGDSPLDRSVEVPITYSPSETIVPESDVEGASPGTKDSALPLTFILDSDTDEEDSPPVESSSATRRGSTVDMEWNRTDGENHSSAVGRDSDTDVDEEGSLQRTLVVAPLAGTQPSSLEDSSTDVEEEGLSVRPVVAQTDIGDSDTDIEEDSVPAAPPVVHLGKVQGPMGGTNNTDIKQEGAPTELSIVHLEKDQHPTGGDRGDSDTDADDNGSLTYMIKSQLPPEEGSRVEWAPATAQGEKTTKKFGAQGRIPITEFEQGLSPFPRVSSVGTLVEPSIQGKSAQVLLEGTQNYVGGSDPDVEGTEDFQNKSDDNSDLDLQDTQCFVEGGNQNHGDGPDEPWEILATQPFCSERSEASETQPIKSYLAAHGACLSPPSTTSGDLQPGSSDQTEPWGNQGTENPKTEIQGQTMESGMENQGIETEIPRVIPERKMEKGPFKRKTLANKTKRSKRKRVSPVVQTEIPEKGQRRTAGRSKNVMIETDNKSCIETKEMERQIPERDTFEGQIPGKETKNLVVEVKVPREMLERGAEGQMAESYDQKAQNSTPVPKSKTGEGDMSELVSNPQILNDGRHQRGLADNKTLPPEEVPMDDQDYSNVPFVASKVPVPEAPTPTSTPRITRSQNRRGSKLLHSSSSLASQESPILGATRSRRQGTQGILPKIESLPSQDSRNKSELSLQSQTQKSFSTSSGPTAPLAPELHTPIPKELPLIPQPHLQTRQNRKRGTTRTCGSTAVSPVLPEHPPPISTDRSETLVPQPRALRSQRARVGETSESLTATPEASYSLTPEAPAPPATRSRRTGASGMPGSTPETQPKLLPGRKRPLATKESSPCPKQPRRGRSQRQESSKEDEEKPMEVEIASKTPVGVAEPAVQAKEEVKGAPSRSLRRARLSREPRAPKVLFTGVVDDRGEQAVLALGGSLASSVAEASHLVTDRVRRTVKFLCALGRGIPILSLEWLHQSRKVGRFLPPEEFVVNDPEQEKNFGFSLREALSRAQERGLLEGYEIFVTPGVQPPPPQMGEIITCCGGSALSSMPRAYKPQRLVISCPQDLSRCSAAARAKLPLLSPEFLLTGVLRQEVQLEAFLLSTPDPVPS

Target ORF information:

RefSeq Version	XM_016430939.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016430939.1

ORF Insert Sequence:

ATGGAGGACA CTCAATTGGT GGACTGGGAG GCTCAGGAGG AGGAGCCTGA GGATTCTAAT 
GGCTCTCCTC CACAATTCGG CTTGGAGCCT GTAGGGCGGC TGCACCTGTT CAGCAGTGTT 
CGAGGTCCTG AGAAAGATTT CCTGTTGTAC CCAGGGGAGA ATGTGGTTGG CCGAATACCA 
GGCTGCTCAG TTGCTTTGCC TTTTCCATCC ATTTCCAAAC ACCATGCAAT AATTGAGATT 
TCAGCCCAAG GCAGGGCACC TATCCTTCGA GATTGTGGGA GCCTCAATCA TACTCGCCTT 
CTAAGACCTC CCAAGCTCCT AAGCCCTGGG GTGGGCCATC AGTTGAGAGA CCAAGATTTG 
GTACTCTTTG CTGACCTGCC TTGCCAGTAT CACCGCCTGG GTACCCCCCA ACTCTCTGGC 
CGACGGGGGG GCCTAAGTGT GGAAGAGACA CCCAGGGTTC CAGGAATAGG AACAGCCCGG 
TTCCAGGGGA CCTTATTGGC TGAAGATTCA GAGGAGGAAG GAGATTCTCC TTTGGATAGG 
AGTGTGGAGG TACCCATCAC ATACTCCCCC TCTGAAACTA TTGTTCCAGA GAGTGATGTG 
GAGGGAGCTT CACCAGGCAC CAAGGATTCT GCCCTGCCTC TTACCTTCAT CTTGGATAGT 
GATACAGATG AAGAAGATTC TCCTCCAGTG GAGTCCTCCT CAGCTACCAG GAGAGGCTCC 
ACTGTGGATA TGGAGTGGAA TAGGACTGAT GGAGAAAACC ATTCCTCAGC TGTGGGGAGG 
GACAGTGATA CAGATGTTGA TGAAGAAGGC AGTCTCCAAA GGACCTTAGT TGTGGCACCT 
TTGGCAGGGA CTCAGCCTTC TTCTCTTGAA GATAGTAGTA CAGATGTGGA AGAGGAGGGG 
CTTTCAGTGA GACCAGTTGT AGCACAAACT GATATAGGGG ACAGTGATAC AGATATAGAA 
GAGGACTCTG TCCCAGCAGC GCCACCTGTA GTCCATCTAG GGAAAGTTCA GGGACCCATG 
GGTGGGACAA ACAATACAGA TATAAAACAG GAGGGGGCCC CAACAGAACT CTCCATAGTG 
CATCTTGAAA AGGACCAGCA CCCTACTGGT GGGGATAGGG GGGACAGTGA CACAGATGCA 
GATGACAATG GATCTCTGAC ATATATGATA AAGAGCCAAC TTCCACCTGA AGAAGGCAGT 
AGGGTAGAGT GGGCCCCAGC CACAGCTCAA GGGGAAAAGA CCACTAAGAA ATTTGGGGCC 
CAGGGTAGGA TCCCTATAAC AGAATTCGAG CAGGGTCTAT CTCCATTCCC AAGGGTCAGC 
TCAGTAGGCA CATTGGTAGA ACCAAGCATT CAAGGGAAGT CAGCTCAGGT ACTTTTGGAA 
GGAACCCAGA ATTATGTTGG AGGTAGTGAT CCAGATGTGG AAGGTACTGA AGACTTTCAG 
AACAAGAGTG ATGACAATTC AGACCTGGAT TTACAAGATA CTCAGTGCTT TGTAGAAGGA 
GGAAATCAGA ACCATGGAGA TGGTCCAGAT GAACCATGGG AAATTTTGGC CACACAGCCA 
TTCTGCTCTG AGCGGTCAGA GGCTTCAGAA ACTCAGCCCA TCAAATCTTA CCTTGCAGCT 
CATGGAGCAT GTCTCTCCCC ACCTAGTACA ACCTCAGGGG ACCTACAGCC AGGGAGTTCA 
GACCAAACAG AGCCCTGGGG GAATCAAGGC ACAGAGAATC CAAAAACAGA GATCCAGGGA 
CAAACTATGG AGAGTGGCAT GGAGAACCAG GGGATAGAAA CAGAGATACC TAGAGTAATA 
CCAGAGAGAA AAATGGAAAA AGGGCCCTTT AAGAGAAAGA CATTGGCAAA TAAGACAAAG 
AGATCCAAAA GAAAAAGAGT GAGTCCAGTG GTACAAACAG AGATACCAGA GAAAGGACAG 
AGACGTACAG CAGGTAGGTC GAAGAATGTT ATGATAGAGA CAGACAACAA AAGCTGTATA 
GAAACAAAGG AAATGGAGAG GCAAATCCCT GAGAGAGACA CATTTGAGGG ACAAATACCA 
GGAAAAGAAA CCAAGAATCT GGTAGTAGAA GTGAAGGTGC CCAGAGAAAT GCTGGAGAGA 
GGGGCAGAGG GACAAATGGC AGAAAGTTAT GACCAGAAGG CCCAGAATTC AACTCCAGTA 
CCAAAATCTA AAACTGGGGA AGGAGACATG TCGGAATTAG TCTCAAACCC CCAAATCCTC 
AATGATGGCA GACATCAGAG AGGTCTTGCT GACAACAAAA CTCTGCCTCC TGAGGAAGTG 
CCAATGGATG ACCAGGACTA CTCAAATGTA CCTTTTGTAG CATCTAAAGT TCCAGTTCCT 
GAAGCCCCAA CTCCTACTTC AACTCCTAGA ATCACTCGGA GTCAAAACAG GAGAGGATCA 
AAACTCCTCC ATTCTTCTTC CTCTCTAGCA TCTCAGGAGT CACCTATATT AGGAGCCACA 
CGAAGCAGGA GGCAGGGAAC CCAGGGGATT CTCCCAAAAA TTGAGTCTCT CCCCTCTCAG 
GATTCTAGAA ACAAATCAGA GCTTTCTCTC CAGAGCCAGA CACAGAAGTC TTTCAGTACC 
TCCTCTGGAC CAACTGCACC CTTGGCTCCT GAACTTCACA CTCCCATCCC TAAAGAATTG 
CCTTTAATCC CTCAACCTCA CCTTCAGACT CGTCAAAACC GGAAACGGGG GACCACCAGA 
ACTTGTGGGT CTACTGCAGT TTCACCAGTC CTCCCTGAGC ACCCCCCTCC CATTTCAACA 
GACAGATCTG AAACTCTTGT TCCCCAACCC AGAGCACTCC GAAGTCAGAG GGCAAGAGTA 
GGAGAAACTT CTGAGTCACT CACGGCCACC CCAGAGGCCT CCTATTCTCT GACACCTGAG 
GCACCTGCTC CTCCAGCTAC CAGGAGTCGA AGAACAGGGG CATCAGGAAT GCCTGGTTCT 
ACTCCAGAGA CCCAGCCTAA GCTGCTTCCA GGCAGAAAGC GGCCTCTAGC CACCAAGGAA 
TCATCTCCAT GCCCAAAGCA ACCCCGGAGA GGACGGTCCC AAAGACAAGA ATCGAGCAAG 
GAAGATGAAG AGAAGCCAAT GGAAGTTGAG ATTGCTTCGA AGACCCCAGT GGGGGTTGCA 
GAACCAGCAG TTCAAGCAAA GGAGGAAGTC AAGGGGGCAC CTAGCCGAAG CTTGCGTCGA 
GCTAGACTCA GTAGAGAACC TCGAGCTCCC AAGGTTCTCT TCACAGGTGT GGTGGATGAT 
CGAGGAGAGC AGGCTGTGCT GGCACTGGGT GGAAGTCTGG CCAGTTCAGT GGCAGAAGCC 
TCACACTTGG TGACAGATCG AGTTAGAAGG ACAGTCAAAT TCCTATGTGC CTTGGGGCGG 
GGGATACCAA TTCTGTCTTT GGAATGGTTG CACCAGTCTA GAAAGGTTGG CCGATTCCTG 
CCACCTGAGG AATTTGTGGT GAATGATCCA GAGCAGGAGA AGAACTTTGG CTTCAGCCTT 
CGAGAGGCAC TAAGCCGGGC CCAGGAGAGG GGCCTACTGG AGGGCTATGA AATCTTTGTA 
ACTCCTGGAG TGCAGCCCCC ACCCCCCCAA ATGGGAGAGA TCATTACCTG CTGTGGAGGC 
TCTGCACTTT CTAGCATGCC CCGGGCCTAT AAGCCCCAGA GACTGGTGAT TTCGTGTCCC 
CAGGACCTCT CCAGATGCTC TGCTGCTGCT AGAGCTAAGC TGCCCCTCCT TTCCCCAGAG 
TTCCTGCTCA CTGGGGTGCT GAGGCAGGAA GTCCAGCTGG AAGCCTTCCT CCTTTCCACC 
CCAGACCCTG TACCTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_007483500.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007483562.1 Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.

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CloneID	OMc11773
Clone ID Related Accession (Same CDS sequence)	XM_016430938.1 , XM_016430939.1 , XM_007483500.2
Accession Version	XM_007483500.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007483500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CTCAATTGGT GGACTGGGAG GCTCAGGAGG AGGAGCCTGA GGATTCTAAT 
GGCTCTCCTC CACAATTCGG CTTGGAGCCT GTAGGGCGGC TGCACCTGTT CAGCAGTGTT 
CGAGGTCCTG AGAAAGATTT CCTGTTGTAC CCAGGGGAGA ATGTGGTTGG CCGAATACCA 
GGCTGCTCAG TTGCTTTGCC TTTTCCATCC ATTTCCAAAC ACCATGCAAT AATTGAGATT 
TCAGCCCAAG GCAGGGCACC TATCCTTCGA GATTGTGGGA GCCTCAATCA TACTCGCCTT 
CTAAGACCTC CCAAGCTCCT AAGCCCTGGG GTGGGCCATC AGTTGAGAGA CCAAGATTTG 
GTACTCTTTG CTGACCTGCC TTGCCAGTAT CACCGCCTGG GTACCCCCCA ACTCTCTGGC 
CGACGGGGGG GCCTAAGTGT GGAAGAGACA CCCAGGGTTC CAGGAATAGG AACAGCCCGG 
TTCCAGGGGA CCTTATTGGC TGAAGATTCA GAGGAGGAAG GAGATTCTCC TTTGGATAGG 
AGTGTGGAGG TACCCATCAC ATACTCCCCC TCTGAAACTA TTGTTCCAGA GAGTGATGTG 
GAGGGAGCTT CACCAGGCAC CAAGGATTCT GCCCTGCCTC TTACCTTCAT CTTGGATAGT 
GATACAGATG AAGAAGATTC TCCTCCAGTG GAGTCCTCCT CAGCTACCAG GAGAGGCTCC 
ACTGTGGATA TGGAGTGGAA TAGGACTGAT GGAGAAAACC ATTCCTCAGC TGTGGGGAGG 
GACAGTGATA CAGATGTTGA TGAAGAAGGC AGTCTCCAAA GGACCTTAGT TGTGGCACCT 
TTGGCAGGGA CTCAGCCTTC TTCTCTTGAA GATAGTAGTA CAGATGTGGA AGAGGAGGGG 
CTTTCAGTGA GACCAGTTGT AGCACAAACT GATATAGGGG ACAGTGATAC AGATATAGAA 
GAGGACTCTG TCCCAGCAGC GCCACCTGTA GTCCATCTAG GGAAAGTTCA GGGACCCATG 
GGTGGGACAA ACAATACAGA TATAAAACAG GAGGGGGCCC CAACAGAACT CTCCATAGTG 
CATCTTGAAA AGGACCAGCA CCCTACTGGT GGGGATAGGG GGGACAGTGA CACAGATGCA 
GATGACAATG GATCTCTGAC ATATATGATA AAGAGCCAAC TTCCACCTGA AGAAGGCAGT 
AGGGTAGAGT GGGCCCCAGC CACAGCTCAA GGGGAAAAGA CCACTAAGAA ATTTGGGGCC 
CAGGGTAGGA TCCCTATAAC AGAATTCGAG CAGGGTCTAT CTCCATTCCC AAGGGTCAGC 
TCAGTAGGCA CATTGGTAGA ACCAAGCATT CAAGGGAAGT CAGCTCAGGT ACTTTTGGAA 
GGAACCCAGA ATTATGTTGG AGGTAGTGAT CCAGATGTGG AAGGTACTGA AGACTTTCAG 
AACAAGAGTG ATGACAATTC AGACCTGGAT TTACAAGATA CTCAGTGCTT TGTAGAAGGA 
GGAAATCAGA ACCATGGAGA TGGTCCAGAT GAACCATGGG AAATTTTGGC CACACAGCCA 
TTCTGCTCTG AGCGGTCAGA GGCTTCAGAA ACTCAGCCCA TCAAATCTTA CCTTGCAGCT 
CATGGAGCAT GTCTCTCCCC ACCTAGTACA ACCTCAGGGG ACCTACAGCC AGGGAGTTCA 
GACCAAACAG AGCCCTGGGG GAATCAAGGC ACAGAGAATC CAAAAACAGA GATCCAGGGA 
CAAACTATGG AGAGTGGCAT GGAGAACCAG GGGATAGAAA CAGAGATACC TAGAGTAATA 
CCAGAGAGAA AAATGGAAAA AGGGCCCTTT AAGAGAAAGA CATTGGCAAA TAAGACAAAG 
AGATCCAAAA GAAAAAGAGT GAGTCCAGTG GTACAAACAG AGATACCAGA GAAAGGACAG 
AGACGTACAG CAGGTAGGTC GAAGAATGTT ATGATAGAGA CAGACAACAA AAGCTGTATA 
GAAACAAAGG AAATGGAGAG GCAAATCCCT GAGAGAGACA CATTTGAGGG ACAAATACCA 
GGAAAAGAAA CCAAGAATCT GGTAGTAGAA GTGAAGGTGC CCAGAGAAAT GCTGGAGAGA 
GGGGCAGAGG GACAAATGGC AGAAAGTTAT GACCAGAAGG CCCAGAATTC AACTCCAGTA 
CCAAAATCTA AAACTGGGGA AGGAGACATG TCGGAATTAG TCTCAAACCC CCAAATCCTC 
AATGATGGCA GACATCAGAG AGGTCTTGCT GACAACAAAA CTCTGCCTCC TGAGGAAGTG 
CCAATGGATG ACCAGGACTA CTCAAATGTA CCTTTTGTAG CATCTAAAGT TCCAGTTCCT 
GAAGCCCCAA CTCCTACTTC AACTCCTAGA ATCACTCGGA GTCAAAACAG GAGAGGATCA 
AAACTCCTCC ATTCTTCTTC CTCTCTAGCA TCTCAGGAGT CACCTATATT AGGAGCCACA 
CGAAGCAGGA GGCAGGGAAC CCAGGGGATT CTCCCAAAAA TTGAGTCTCT CCCCTCTCAG 
GATTCTAGAA ACAAATCAGA GCTTTCTCTC CAGAGCCAGA CACAGAAGTC TTTCAGTACC 
TCCTCTGGAC CAACTGCACC CTTGGCTCCT GAACTTCACA CTCCCATCCC TAAAGAATTG 
CCTTTAATCC CTCAACCTCA CCTTCAGACT CGTCAAAACC GGAAACGGGG GACCACCAGA 
ACTTGTGGGT CTACTGCAGT TTCACCAGTC CTCCCTGAGC ACCCCCCTCC CATTTCAACA 
GACAGATCTG AAACTCTTGT TCCCCAACCC AGAGCACTCC GAAGTCAGAG GGCAAGAGTA 
GGAGAAACTT CTGAGTCACT CACGGCCACC CCAGAGGCCT CCTATTCTCT GACACCTGAG 
GCACCTGCTC CTCCAGCTAC CAGGAGTCGA AGAACAGGGG CATCAGGAAT GCCTGGTTCT 
ACTCCAGAGA CCCAGCCTAA GCTGCTTCCA GGCAGAAAGC GGCCTCTAGC CACCAAGGAA 
TCATCTCCAT GCCCAAAGCA ACCCCGGAGA GGACGGTCCC AAAGACAAGA ATCGAGCAAG 
GAAGATGAAG AGAAGCCAAT GGAAGTTGAG ATTGCTTCGA AGACCCCAGT GGGGGTTGCA 
GAACCAGCAG TTCAAGCAAA GGAGGAAGTC AAGGGGGCAC CTAGCCGAAG CTTGCGTCGA 
GCTAGACTCA GTAGAGAACC TCGAGCTCCC AAGGTTCTCT TCACAGGTGT GGTGGATGAT 
CGAGGAGAGC AGGCTGTGCT GGCACTGGGT GGAAGTCTGG CCAGTTCAGT GGCAGAAGCC 
TCACACTTGG TGACAGATCG AGTTAGAAGG ACAGTCAAAT TCCTATGTGC CTTGGGGCGG 
GGGATACCAA TTCTGTCTTT GGAATGGTTG CACCAGTCTA GAAAGGTTGG CCGATTCCTG 
CCACCTGAGG AATTTGTGGT GAATGATCCA GAGCAGGAGA AGAACTTTGG CTTCAGCCTT 
CGAGAGGCAC TAAGCCGGGC CCAGGAGAGG GGCCTACTGG AGGGCTATGA AATCTTTGTA 
ACTCCTGGAG TGCAGCCCCC ACCCCCCCAA ATGGGAGAGA TCATTACCTG CTGTGGAGGC 
TCTGCACTTT CTAGCATGCC CCGGGCCTAT AAGCCCCAGA GACTGGTGAT TTCGTGTCCC 
CAGGACCTCT CCAGATGCTC TGCTGCTGCT AGAGCTAAGC TGCCCCTCCT TTCCCCAGAG 
TTCCTGCTCA CTGGGGTGCT GAGGCAGGAA GTCCAGCTGG AAGCCTTCCT CCTTTCCACC 
CCAGACCCTG TACCTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007483562.1
CDS	234..4034
Translation	MEDTQLVDWEAQEEEPEDSNGSPPQFGLEPVGRLHLFSSVRGPEKDFLLYPGENVVGRIPGCSVALPFPSISKHHAIIEISAQGRAPILRDCGSLNHTRLLRPPKLLSPGVGHQLRDQDLVLFADLPCQYHRLGTPQLSGRRGGLSVEETPRVPGIGTARFQGTLLAEDSEEEGDSPLDRSVEVPITYSPSETIVPESDVEGASPGTKDSALPLTFILDSDTDEEDSPPVESSSATRRGSTVDMEWNRTDGENHSSAVGRDSDTDVDEEGSLQRTLVVAPLAGTQPSSLEDSSTDVEEEGLSVRPVVAQTDIGDSDTDIEEDSVPAAPPVVHLGKVQGPMGGTNNTDIKQEGAPTELSIVHLEKDQHPTGGDRGDSDTDADDNGSLTYMIKSQLPPEEGSRVEWAPATAQGEKTTKKFGAQGRIPITEFEQGLSPFPRVSSVGTLVEPSIQGKSAQVLLEGTQNYVGGSDPDVEGTEDFQNKSDDNSDLDLQDTQCFVEGGNQNHGDGPDEPWEILATQPFCSERSEASETQPIKSYLAAHGACLSPPSTTSGDLQPGSSDQTEPWGNQGTENPKTEIQGQTMESGMENQGIETEIPRVIPERKMEKGPFKRKTLANKTKRSKRKRVSPVVQTEIPEKGQRRTAGRSKNVMIETDNKSCIETKEMERQIPERDTFEGQIPGKETKNLVVEVKVPREMLERGAEGQMAESYDQKAQNSTPVPKSKTGEGDMSELVSNPQILNDGRHQRGLADNKTLPPEEVPMDDQDYSNVPFVASKVPVPEAPTPTSTPRITRSQNRRGSKLLHSSSSLASQESPILGATRSRRQGTQGILPKIESLPSQDSRNKSELSLQSQTQKSFSTSSGPTAPLAPELHTPIPKELPLIPQPHLQTRQNRKRGTTRTCGSTAVSPVLPEHPPPISTDRSETLVPQPRALRSQRARVGETSESLTATPEASYSLTPEAPAPPATRSRRTGASGMPGSTPETQPKLLPGRKRPLATKESSPCPKQPRRGRSQRQESSKEDEEKPMEVEIASKTPVGVAEPAVQAKEEVKGAPSRSLRRARLSREPRAPKVLFTGVVDDRGEQAVLALGGSLASSVAEASHLVTDRVRRTVKFLCALGRGIPILSLEWLHQSRKVGRFLPPEEFVVNDPEQEKNFGFSLREALSRAQERGLLEGYEIFVTPGVQPPPPQMGEIITCCGGSALSSMPRAYKPQRLVISCPQDLSRCSAAARAKLPLLSPEFLLTGVLRQEVQLEAFLLSTPDPVPS

Target ORF information:

RefSeq Version	XM_007483500.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007483500.2

ORF Insert Sequence:

ATGGAGGACA CTCAATTGGT GGACTGGGAG GCTCAGGAGG AGGAGCCTGA GGATTCTAAT 
GGCTCTCCTC CACAATTCGG CTTGGAGCCT GTAGGGCGGC TGCACCTGTT CAGCAGTGTT 
CGAGGTCCTG AGAAAGATTT CCTGTTGTAC CCAGGGGAGA ATGTGGTTGG CCGAATACCA 
GGCTGCTCAG TTGCTTTGCC TTTTCCATCC ATTTCCAAAC ACCATGCAAT AATTGAGATT 
TCAGCCCAAG GCAGGGCACC TATCCTTCGA GATTGTGGGA GCCTCAATCA TACTCGCCTT 
CTAAGACCTC CCAAGCTCCT AAGCCCTGGG GTGGGCCATC AGTTGAGAGA CCAAGATTTG 
GTACTCTTTG CTGACCTGCC TTGCCAGTAT CACCGCCTGG GTACCCCCCA ACTCTCTGGC 
CGACGGGGGG GCCTAAGTGT GGAAGAGACA CCCAGGGTTC CAGGAATAGG AACAGCCCGG 
TTCCAGGGGA CCTTATTGGC TGAAGATTCA GAGGAGGAAG GAGATTCTCC TTTGGATAGG 
AGTGTGGAGG TACCCATCAC ATACTCCCCC TCTGAAACTA TTGTTCCAGA GAGTGATGTG 
GAGGGAGCTT CACCAGGCAC CAAGGATTCT GCCCTGCCTC TTACCTTCAT CTTGGATAGT 
GATACAGATG AAGAAGATTC TCCTCCAGTG GAGTCCTCCT CAGCTACCAG GAGAGGCTCC 
ACTGTGGATA TGGAGTGGAA TAGGACTGAT GGAGAAAACC ATTCCTCAGC TGTGGGGAGG 
GACAGTGATA CAGATGTTGA TGAAGAAGGC AGTCTCCAAA GGACCTTAGT TGTGGCACCT 
TTGGCAGGGA CTCAGCCTTC TTCTCTTGAA GATAGTAGTA CAGATGTGGA AGAGGAGGGG 
CTTTCAGTGA GACCAGTTGT AGCACAAACT GATATAGGGG ACAGTGATAC AGATATAGAA 
GAGGACTCTG TCCCAGCAGC GCCACCTGTA GTCCATCTAG GGAAAGTTCA GGGACCCATG 
GGTGGGACAA ACAATACAGA TATAAAACAG GAGGGGGCCC CAACAGAACT CTCCATAGTG 
CATCTTGAAA AGGACCAGCA CCCTACTGGT GGGGATAGGG GGGACAGTGA CACAGATGCA 
GATGACAATG GATCTCTGAC ATATATGATA AAGAGCCAAC TTCCACCTGA AGAAGGCAGT 
AGGGTAGAGT GGGCCCCAGC CACAGCTCAA GGGGAAAAGA CCACTAAGAA ATTTGGGGCC 
CAGGGTAGGA TCCCTATAAC AGAATTCGAG CAGGGTCTAT CTCCATTCCC AAGGGTCAGC 
TCAGTAGGCA CATTGGTAGA ACCAAGCATT CAAGGGAAGT CAGCTCAGGT ACTTTTGGAA 
GGAACCCAGA ATTATGTTGG AGGTAGTGAT CCAGATGTGG AAGGTACTGA AGACTTTCAG 
AACAAGAGTG ATGACAATTC AGACCTGGAT TTACAAGATA CTCAGTGCTT TGTAGAAGGA 
GGAAATCAGA ACCATGGAGA TGGTCCAGAT GAACCATGGG AAATTTTGGC CACACAGCCA 
TTCTGCTCTG AGCGGTCAGA GGCTTCAGAA ACTCAGCCCA TCAAATCTTA CCTTGCAGCT 
CATGGAGCAT GTCTCTCCCC ACCTAGTACA ACCTCAGGGG ACCTACAGCC AGGGAGTTCA 
GACCAAACAG AGCCCTGGGG GAATCAAGGC ACAGAGAATC CAAAAACAGA GATCCAGGGA 
CAAACTATGG AGAGTGGCAT GGAGAACCAG GGGATAGAAA CAGAGATACC TAGAGTAATA 
CCAGAGAGAA AAATGGAAAA AGGGCCCTTT AAGAGAAAGA CATTGGCAAA TAAGACAAAG 
AGATCCAAAA GAAAAAGAGT GAGTCCAGTG GTACAAACAG AGATACCAGA GAAAGGACAG 
AGACGTACAG CAGGTAGGTC GAAGAATGTT ATGATAGAGA CAGACAACAA AAGCTGTATA 
GAAACAAAGG AAATGGAGAG GCAAATCCCT GAGAGAGACA CATTTGAGGG ACAAATACCA 
GGAAAAGAAA CCAAGAATCT GGTAGTAGAA GTGAAGGTGC CCAGAGAAAT GCTGGAGAGA 
GGGGCAGAGG GACAAATGGC AGAAAGTTAT GACCAGAAGG CCCAGAATTC AACTCCAGTA 
CCAAAATCTA AAACTGGGGA AGGAGACATG TCGGAATTAG TCTCAAACCC CCAAATCCTC 
AATGATGGCA GACATCAGAG AGGTCTTGCT GACAACAAAA CTCTGCCTCC TGAGGAAGTG 
CCAATGGATG ACCAGGACTA CTCAAATGTA CCTTTTGTAG CATCTAAAGT TCCAGTTCCT 
GAAGCCCCAA CTCCTACTTC AACTCCTAGA ATCACTCGGA GTCAAAACAG GAGAGGATCA 
AAACTCCTCC ATTCTTCTTC CTCTCTAGCA TCTCAGGAGT CACCTATATT AGGAGCCACA 
CGAAGCAGGA GGCAGGGAAC CCAGGGGATT CTCCCAAAAA TTGAGTCTCT CCCCTCTCAG 
GATTCTAGAA ACAAATCAGA GCTTTCTCTC CAGAGCCAGA CACAGAAGTC TTTCAGTACC 
TCCTCTGGAC CAACTGCACC CTTGGCTCCT GAACTTCACA CTCCCATCCC TAAAGAATTG 
CCTTTAATCC CTCAACCTCA CCTTCAGACT CGTCAAAACC GGAAACGGGG GACCACCAGA 
ACTTGTGGGT CTACTGCAGT TTCACCAGTC CTCCCTGAGC ACCCCCCTCC CATTTCAACA 
GACAGATCTG AAACTCTTGT TCCCCAACCC AGAGCACTCC GAAGTCAGAG GGCAAGAGTA 
GGAGAAACTT CTGAGTCACT CACGGCCACC CCAGAGGCCT CCTATTCTCT GACACCTGAG 
GCACCTGCTC CTCCAGCTAC CAGGAGTCGA AGAACAGGGG CATCAGGAAT GCCTGGTTCT 
ACTCCAGAGA CCCAGCCTAA GCTGCTTCCA GGCAGAAAGC GGCCTCTAGC CACCAAGGAA 
TCATCTCCAT GCCCAAAGCA ACCCCGGAGA GGACGGTCCC AAAGACAAGA ATCGAGCAAG 
GAAGATGAAG AGAAGCCAAT GGAAGTTGAG ATTGCTTCGA AGACCCCAGT GGGGGTTGCA 
GAACCAGCAG TTCAAGCAAA GGAGGAAGTC AAGGGGGCAC CTAGCCGAAG CTTGCGTCGA 
GCTAGACTCA GTAGAGAACC TCGAGCTCCC AAGGTTCTCT TCACAGGTGT GGTGGATGAT 
CGAGGAGAGC AGGCTGTGCT GGCACTGGGT GGAAGTCTGG CCAGTTCAGT GGCAGAAGCC 
TCACACTTGG TGACAGATCG AGTTAGAAGG ACAGTCAAAT TCCTATGTGC CTTGGGGCGG 
GGGATACCAA TTCTGTCTTT GGAATGGTTG CACCAGTCTA GAAAGGTTGG CCGATTCCTG 
CCACCTGAGG AATTTGTGGT GAATGATCCA GAGCAGGAGA AGAACTTTGG CTTCAGCCTT 
CGAGAGGCAC TAAGCCGGGC CCAGGAGAGG GGCCTACTGG AGGGCTATGA AATCTTTGTA 
ACTCCTGGAG TGCAGCCCCC ACCCCCCCAA ATGGGAGAGA TCATTACCTG CTGTGGAGGC 
TCTGCACTTT CTAGCATGCC CCGGGCCTAT AAGCCCCAGA GACTGGTGAT TTCGTGTCCC 
CAGGACCTCT CCAGATGCTC TGCTGCTGCT AGAGCTAAGC TGCCCCTCCT TTCCCCAGAG 
TTCCTGCTCA CTGGGGTGCT GAGGCAGGAA GTCCAGCTGG AAGCCTTCCT CCTTTCCACC 
CCAGACCCTG TACCTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_007483501.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007483563.1 Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.

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CloneID	OMc11774
Clone ID Related Accession (Same CDS sequence)	XM_007483501.2
Accession Version	XM_007483501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	mediator of DNA damage checkpoint protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007483501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CTCAATTGGT GGACTGGGAG GCTCAGGAGG AGGAGCCTGA GGATTCTAAT 
GGCTCTCCTC CACAATTCGG CTTGGAGCCT GTAGGGCGGC TGCACCTGTT CAGCAGTGTT 
CGAGGTCCTG AGAAAGATTT CCTGTTGTAC CCAGGGGAGA ATGTGGTTGG CCGAATACCA 
GGCTGCTCAG TTGCTTTGCC TTTTCCATCC ATTTCCAAAC ACCATGCAAT AATTGAGATT 
TCAGCCCAAG GCAGGGCACC TATCCTTCGA GATTGTGGGA GCCTCAATCA TACTCGCCTT 
CTAAGACCTC CCAAGCTCCT AAGCCCTGGG GTGGGCCATC AGTTGAGAGA CCAAGATTTG 
GTACTCTTTG CTGACCTGCC TTGCCAGTAT CACCGCCTGG GTACCCCCCA ACTCTCTGGC 
CGACGGGGGG GCCTAAGTGT GGAAGAGACA CCCAGGGTTC CAGGAATAGG AACAGCCCGG 
TTCCAGGGGA CCTTATTGGC TGAAGATTCA GAGGAGGAAG GAGATTCTCC TTTGGATAGG 
AGTGTGGAGG TACCCATCAC ATACTCCCCC TCTGAAACTA TTGTTCCAGA GAGTGATGTG 
GAGGGAGCTT CACCAGGCAC CAAGGATTCT GCCCTGCCTC TTACCTTCAT CTTGGATAGT 
GATACAGATG AAGAAGATTC TCCTCCAGTG GAGTCCTCCT CAGCTACCAG GAGAGGCTCC 
ACTGTGGATA TGGAGTGGAA TAGGACTGAT GGAGAAAACC ATTCCTCAGC TGTGGGGAGG 
GACAGTGATA CAGATGTTGA TGAAGAAGGC AGTCTCCAAA GGACCTTAGT TGTGGCACCT 
TTGGCAGGGA CTCAGCCTTC TTCTCTTGAA GATAGTAGTA CAGATGTGGA AGAGGAGGGG 
CTTTCAGTGA GACCAGTTGT AGCACAAACT GATATAGGGG ACAGTGATAC AGATATAGAA 
GAGGACTCTG TCCCAGCAGC GCCACCTGTA GTCCATCTAG GGAAAGTTCA GGGACCCATG 
GGTGGGACAA ACAATACAGA TATAAAACAG GAGGGGGCCC CAACAGAACT CTCCATAGTG 
CATCTTGAAA AGGACCAGCA CCCTACTGGT GGGGATAGGG GGGACAGTGA CACAGATGCA 
GATGACAATG GATCTCTGAC ATATATGATA AAGAGCCAAC TTCCACCTGA AGAAGGCAGT 
AGGGTAGAGT GGGCCCCAGC CACAGCTCAA GGGGAAAAGA CCACTAAGAA ATTTGGGGCC 
CAGGGTAGGA TCCCTATAAC AGAATTCGAG CAGGGTCTAT CTCCATTCCC AAGGGTCAGC 
TCAGTAGGCA CATTGGTAGA ACCAAGCATT CAAGGGAAGT CAGCTCAGGT ACTTTTGGAA 
GGAACCCAGA ATTATGTTGG AGGTAGTGAT CCAGATGTGG AAGGTACTGA AGACTTTCAG 
AACAAGAGTG ATGACAATTC AGACCTGGAT TTACAAGATA CTCAGTGCTT TGTAGAAGGA 
GGAAATCAGA ACCATGGAGA TGGTCCAGAT GAACCATGGG AAATTTTGGC CACACAGCCA 
TTCTGCTCTG AGCGGTCAGA GGCTTCAGAA ACTCAGCCCA TCAAATCTTA CCTTGCAGCT 
CATGGAGCAT GTCTCTCCCC ACCTAGTACA ACCTCAGGGG ACCTACAGCC AGGGAGTTCA 
GACCAAACAG AGCCCTGGGG GAATCAAGGC ACAGAGAATC CAAAAACAGA GATCCAGGGA 
CAAACTATGG AGAGTGGCAT GGAGAACCAG GGGATAGAAA CAGAGATACC TAGAGTAATA 
CCAGAGAGAA AAATGGAAAA AGGGCCCTTT AAGAGAAAGA CATTGGCAAA TAAGACAAAG 
AGATCCAAAA GAAAAAGAGT GAGTCCAGTG GTACAAACAG AGATACCAGA GAAAGGACAG 
AGACGTACAG CAGGTAGGTC GAAGAATGTT ATGATAGAGA CAGACAACAA AAGCTGTATA 
GAAACAAAGG AAATGGAGAG GCAAATCCCT GAGAGAGACA CATTTGAGGG ACAAATACCA 
GGAAAAGAAA CCAAGAATCT GGTAGTAGAA GTGAAGGTGC CCAGAGAAAT GCTGGAGAGA 
GGGGCAGAGG GACAAATGGC AGAAAGTTAT GACCAGAAGG CCCAGAATTC AACTCCAGTA 
CCAAAATCTA AAACTGGGGA AGGAGACATG TCGGAATTAG TCTCAAACCC CCAAATCCTC 
AATGATGGCA GACATCAGAG AGGTCTTGCT GACAACAAAA CTCTGCCTCC TGAGGAAGTG 
CCAATGGATG ACCAGGACTA CTCAAATGTA CCTTTTGTAG CATCTAAAGT TCCAGTTCCT 
GAAGCCCCAA CTCCTACTTC AACTCCTAGA ATCACTCGGA GTCAAAACAG GAGAGGATCA 
AAACTCCTCC ATTCTTCTTC CTCTCTAGCA TCTCAGGAGT CACCTATATT AGGAGCCACA 
CGAAGCAGGA GGCAGGGAAC CCAGGGGATT CTCCCAAAAA TTGAGTCTCT CCCCTCTCAG 
GATTCTAGAA ACAAATCAGA GCTTTCTCTC CAGAGCCAGA CACAGAAGTC TTTCAGTACC 
TCCTCTGGAC CAACTGCACC CTTGGCTCCT GAACTTCACA CTCCCATCCC TAAAGAATTG 
CCTTTAATCC CTCAACCTCA CCTTCAGACT CGTCAAAACC GGAAACGGGG GACCACCAGA 
ACTTGTGGGT CTACTGCAGT TTCACCAGTC CTCCCTGAGC ACCCCCCTCC CATTTCAACA 
GACAGATCTG AAACTCTTGT TCCCCAACCC AGAGCACTCC GAAGTCAGAG GGCAAGAGTA 
GGAGAAACTT CTGAGTCACT CACGGCCACC CCAGAGGCCT CCTATTCTCT GACACCTGAG 
GCACCTGCTC CTCCAGCTAC CAGGAGTCGA AGAACAGGGG CATCAGGAAT GCCTGGTTCT 
ACTCCAGAGA CCCAGCCTAA GCTGCTTCCA GGCAGAAAGC GGCCTCTAGC CACCAAGGAA 
TCATCTCCAT GCCCAAAGCA ACCCCGGAGA GGACGGTCCC AAAGACAAGA ATCGAGCAAG 
GAAGATGAAG AGAAGCCAAT GGAAGTTGAG ATTGCTTCGA AGACCCCAGT GGGGGTTGCA 
GAACCAGCAG TTCAAGCAAA GGAGGAAGTC AAGGGGGCAC CTAGCCGAAG CTTGCGTCGA 
GCTAGACTCA GTAGAGAACC TCGAGCTCCC AAGGTTCTCT TCACAGGTGT GGTGGATGAT 
CGAGGAGAGC AGGCTGTGCT GGCACTGGGT GGAAGTCTGG CCAGTTCAGT GGCAGAAGCC 
TCACACTTGG TGACAGATCG AGTTAGAAGG ACAGTCAAAT TCCTATGTGC CTTGGGGCGG 
GGGATACCAA TTCTGTCTTT GGAATGGTTG CACCAGTCTA GAAAGGTTGG CCGATTCCTG 
CCACCTGAGG AATTTGTGGT GAATGATCCA GAGCAGGAGA AGAACTTTGG CTTCAGCCTT 
CGAGAGGCAC TAAGCCGGGC CCAGGAGAGG GGCCTACTGG AGCCCCAGAG ACTGGTGATT 
TCGTGTCCCC AGGACCTCTC CAGATGCTCT GCTGCTGCTA GAGCTAAGCT GCCCCTCCTT 
TCCCCAGAGT TCCTGCTCAC TGGGGTGCTG AGGCAGGAAG TCCAGCTGGA AGCCTTCCTC 
CTTTCCACCC CAGACCCTGT ACCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007483563.1
CDS	572..4261
Translation	MEDTQLVDWEAQEEEPEDSNGSPPQFGLEPVGRLHLFSSVRGPEKDFLLYPGENVVGRIPGCSVALPFPSISKHHAIIEISAQGRAPILRDCGSLNHTRLLRPPKLLSPGVGHQLRDQDLVLFADLPCQYHRLGTPQLSGRRGGLSVEETPRVPGIGTARFQGTLLAEDSEEEGDSPLDRSVEVPITYSPSETIVPESDVEGASPGTKDSALPLTFILDSDTDEEDSPPVESSSATRRGSTVDMEWNRTDGENHSSAVGRDSDTDVDEEGSLQRTLVVAPLAGTQPSSLEDSSTDVEEEGLSVRPVVAQTDIGDSDTDIEEDSVPAAPPVVHLGKVQGPMGGTNNTDIKQEGAPTELSIVHLEKDQHPTGGDRGDSDTDADDNGSLTYMIKSQLPPEEGSRVEWAPATAQGEKTTKKFGAQGRIPITEFEQGLSPFPRVSSVGTLVEPSIQGKSAQVLLEGTQNYVGGSDPDVEGTEDFQNKSDDNSDLDLQDTQCFVEGGNQNHGDGPDEPWEILATQPFCSERSEASETQPIKSYLAAHGACLSPPSTTSGDLQPGSSDQTEPWGNQGTENPKTEIQGQTMESGMENQGIETEIPRVIPERKMEKGPFKRKTLANKTKRSKRKRVSPVVQTEIPEKGQRRTAGRSKNVMIETDNKSCIETKEMERQIPERDTFEGQIPGKETKNLVVEVKVPREMLERGAEGQMAESYDQKAQNSTPVPKSKTGEGDMSELVSNPQILNDGRHQRGLADNKTLPPEEVPMDDQDYSNVPFVASKVPVPEAPTPTSTPRITRSQNRRGSKLLHSSSSLASQESPILGATRSRRQGTQGILPKIESLPSQDSRNKSELSLQSQTQKSFSTSSGPTAPLAPELHTPIPKELPLIPQPHLQTRQNRKRGTTRTCGSTAVSPVLPEHPPPISTDRSETLVPQPRALRSQRARVGETSESLTATPEASYSLTPEAPAPPATRSRRTGASGMPGSTPETQPKLLPGRKRPLATKESSPCPKQPRRGRSQRQESSKEDEEKPMEVEIASKTPVGVAEPAVQAKEEVKGAPSRSLRRARLSREPRAPKVLFTGVVDDRGEQAVLALGGSLASSVAEASHLVTDRVRRTVKFLCALGRGIPILSLEWLHQSRKVGRFLPPEEFVVNDPEQEKNFGFSLREALSRAQERGLLEPQRLVISCPQDLSRCSAAARAKLPLLSPEFLLTGVLRQEVQLEAFLLSTPDPVPS

Target ORF information:

RefSeq Version	XM_007483501.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007483501.2

ORF Insert Sequence:

ATGGAGGACA CTCAATTGGT GGACTGGGAG GCTCAGGAGG AGGAGCCTGA GGATTCTAAT 
GGCTCTCCTC CACAATTCGG CTTGGAGCCT GTAGGGCGGC TGCACCTGTT CAGCAGTGTT 
CGAGGTCCTG AGAAAGATTT CCTGTTGTAC CCAGGGGAGA ATGTGGTTGG CCGAATACCA 
GGCTGCTCAG TTGCTTTGCC TTTTCCATCC ATTTCCAAAC ACCATGCAAT AATTGAGATT 
TCAGCCCAAG GCAGGGCACC TATCCTTCGA GATTGTGGGA GCCTCAATCA TACTCGCCTT 
CTAAGACCTC CCAAGCTCCT AAGCCCTGGG GTGGGCCATC AGTTGAGAGA CCAAGATTTG 
GTACTCTTTG CTGACCTGCC TTGCCAGTAT CACCGCCTGG GTACCCCCCA ACTCTCTGGC 
CGACGGGGGG GCCTAAGTGT GGAAGAGACA CCCAGGGTTC CAGGAATAGG AACAGCCCGG 
TTCCAGGGGA CCTTATTGGC TGAAGATTCA GAGGAGGAAG GAGATTCTCC TTTGGATAGG 
AGTGTGGAGG TACCCATCAC ATACTCCCCC TCTGAAACTA TTGTTCCAGA GAGTGATGTG 
GAGGGAGCTT CACCAGGCAC CAAGGATTCT GCCCTGCCTC TTACCTTCAT CTTGGATAGT 
GATACAGATG AAGAAGATTC TCCTCCAGTG GAGTCCTCCT CAGCTACCAG GAGAGGCTCC 
ACTGTGGATA TGGAGTGGAA TAGGACTGAT GGAGAAAACC ATTCCTCAGC TGTGGGGAGG 
GACAGTGATA CAGATGTTGA TGAAGAAGGC AGTCTCCAAA GGACCTTAGT TGTGGCACCT 
TTGGCAGGGA CTCAGCCTTC TTCTCTTGAA GATAGTAGTA CAGATGTGGA AGAGGAGGGG 
CTTTCAGTGA GACCAGTTGT AGCACAAACT GATATAGGGG ACAGTGATAC AGATATAGAA 
GAGGACTCTG TCCCAGCAGC GCCACCTGTA GTCCATCTAG GGAAAGTTCA GGGACCCATG 
GGTGGGACAA ACAATACAGA TATAAAACAG GAGGGGGCCC CAACAGAACT CTCCATAGTG 
CATCTTGAAA AGGACCAGCA CCCTACTGGT GGGGATAGGG GGGACAGTGA CACAGATGCA 
GATGACAATG GATCTCTGAC ATATATGATA AAGAGCCAAC TTCCACCTGA AGAAGGCAGT 
AGGGTAGAGT GGGCCCCAGC CACAGCTCAA GGGGAAAAGA CCACTAAGAA ATTTGGGGCC 
CAGGGTAGGA TCCCTATAAC AGAATTCGAG CAGGGTCTAT CTCCATTCCC AAGGGTCAGC 
TCAGTAGGCA CATTGGTAGA ACCAAGCATT CAAGGGAAGT CAGCTCAGGT ACTTTTGGAA 
GGAACCCAGA ATTATGTTGG AGGTAGTGAT CCAGATGTGG AAGGTACTGA AGACTTTCAG 
AACAAGAGTG ATGACAATTC AGACCTGGAT TTACAAGATA CTCAGTGCTT TGTAGAAGGA 
GGAAATCAGA ACCATGGAGA TGGTCCAGAT GAACCATGGG AAATTTTGGC CACACAGCCA 
TTCTGCTCTG AGCGGTCAGA GGCTTCAGAA ACTCAGCCCA TCAAATCTTA CCTTGCAGCT 
CATGGAGCAT GTCTCTCCCC ACCTAGTACA ACCTCAGGGG ACCTACAGCC AGGGAGTTCA 
GACCAAACAG AGCCCTGGGG GAATCAAGGC ACAGAGAATC CAAAAACAGA GATCCAGGGA 
CAAACTATGG AGAGTGGCAT GGAGAACCAG GGGATAGAAA CAGAGATACC TAGAGTAATA 
CCAGAGAGAA AAATGGAAAA AGGGCCCTTT AAGAGAAAGA CATTGGCAAA TAAGACAAAG 
AGATCCAAAA GAAAAAGAGT GAGTCCAGTG GTACAAACAG AGATACCAGA GAAAGGACAG 
AGACGTACAG CAGGTAGGTC GAAGAATGTT ATGATAGAGA CAGACAACAA AAGCTGTATA 
GAAACAAAGG AAATGGAGAG GCAAATCCCT GAGAGAGACA CATTTGAGGG ACAAATACCA 
GGAAAAGAAA CCAAGAATCT GGTAGTAGAA GTGAAGGTGC CCAGAGAAAT GCTGGAGAGA 
GGGGCAGAGG GACAAATGGC AGAAAGTTAT GACCAGAAGG CCCAGAATTC AACTCCAGTA 
CCAAAATCTA AAACTGGGGA AGGAGACATG TCGGAATTAG TCTCAAACCC CCAAATCCTC 
AATGATGGCA GACATCAGAG AGGTCTTGCT GACAACAAAA CTCTGCCTCC TGAGGAAGTG 
CCAATGGATG ACCAGGACTA CTCAAATGTA CCTTTTGTAG CATCTAAAGT TCCAGTTCCT 
GAAGCCCCAA CTCCTACTTC AACTCCTAGA ATCACTCGGA GTCAAAACAG GAGAGGATCA 
AAACTCCTCC ATTCTTCTTC CTCTCTAGCA TCTCAGGAGT CACCTATATT AGGAGCCACA 
CGAAGCAGGA GGCAGGGAAC CCAGGGGATT CTCCCAAAAA TTGAGTCTCT CCCCTCTCAG 
GATTCTAGAA ACAAATCAGA GCTTTCTCTC CAGAGCCAGA CACAGAAGTC TTTCAGTACC 
TCCTCTGGAC CAACTGCACC CTTGGCTCCT GAACTTCACA CTCCCATCCC TAAAGAATTG 
CCTTTAATCC CTCAACCTCA CCTTCAGACT CGTCAAAACC GGAAACGGGG GACCACCAGA 
ACTTGTGGGT CTACTGCAGT TTCACCAGTC CTCCCTGAGC ACCCCCCTCC CATTTCAACA 
GACAGATCTG AAACTCTTGT TCCCCAACCC AGAGCACTCC GAAGTCAGAG GGCAAGAGTA 
GGAGAAACTT CTGAGTCACT CACGGCCACC CCAGAGGCCT CCTATTCTCT GACACCTGAG 
GCACCTGCTC CTCCAGCTAC CAGGAGTCGA AGAACAGGGG CATCAGGAAT GCCTGGTTCT 
ACTCCAGAGA CCCAGCCTAA GCTGCTTCCA GGCAGAAAGC GGCCTCTAGC CACCAAGGAA 
TCATCTCCAT GCCCAAAGCA ACCCCGGAGA GGACGGTCCC AAAGACAAGA ATCGAGCAAG 
GAAGATGAAG AGAAGCCAAT GGAAGTTGAG ATTGCTTCGA AGACCCCAGT GGGGGTTGCA 
GAACCAGCAG TTCAAGCAAA GGAGGAAGTC AAGGGGGCAC CTAGCCGAAG CTTGCGTCGA 
GCTAGACTCA GTAGAGAACC TCGAGCTCCC AAGGTTCTCT TCACAGGTGT GGTGGATGAT 
CGAGGAGAGC AGGCTGTGCT GGCACTGGGT GGAAGTCTGG CCAGTTCAGT GGCAGAAGCC 
TCACACTTGG TGACAGATCG AGTTAGAAGG ACAGTCAAAT TCCTATGTGC CTTGGGGCGG 
GGGATACCAA TTCTGTCTTT GGAATGGTTG CACCAGTCTA GAAAGGTTGG CCGATTCCTG 
CCACCTGAGG AATTTGTGGT GAATGATCCA GAGCAGGAGA AGAACTTTGG CTTCAGCCTT 
CGAGAGGCAC TAAGCCGGGC CCAGGAGAGG GGCCTACTGG AGCCCCAGAG ACTGGTGATT 
TCGTGTCCCC AGGACCTCTC CAGATGCTCT GCTGCTGCTA GAGCTAAGCT GCCCCTCCTT 
TCCCCAGAGT TCCTGCTCAC TGGGGTGCTG AGGCAGGAAG TCCAGCTGGA AGCCTTCCTC 
CTTTCCACCC CAGACCCTGT ACCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MDC1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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