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a Related Biological Terms:

aldimine form

(see quinimine form)

aldol condensation

The reversible formation of a bond between two carbons, one of a carbonyl group and the other adjacent to a second carbonyl group; by extension, a condensation of compounds that share that kind of chemistry, e.g. the citrate synthase reaction.

aliquot

A representative sample of a fixed proportion. Thus 50 µl from a 25 ml solution represents 1/500 of the total.

alkaline Bohr effect

(see Bohr effect)

all-or-none assay

A technique to measure the total amount of a functional enzyme, regardless of its efficiency or affinity for its substrate.

allele

One member of a pair of homologous genes in a diploid cell. An individual with identical alleles at a genetic locus is a homozygote; one with non-identical alleles is a heterozygote. In a case in which one allele leads to an observable gene product and the other has no phenotype, the functional allele is said to be dominant and the non-functional allele recessive.

allele-specific PCR

(see PCR amplification of specific alleles)

allele-specific PCR (ASPCR)

The adaptation of a PCR primer to selectivity for only one variant sequence. The 3'-end of the primer is designed to be complementary to only one allelic sequence, so the appearance of a PCR fragment is indicative of the presence of the variant sequence.Ugozzoli, L. and Wallace, R.B. (1991) Methods Companion Methods Enzymol. 2, 42-48

allele-specific amplification

(see PCR amplification of specific alleles)

allelic exclusion

(see imprinting)

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