Resources

List by Alphabet: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

gene-trapping

An approach that randomly disrupts genes throughout the genome by inserting a DNA element (usually containing a reporter). These DNA elements are sometimes fused in frame of the endogenous gene so that the reporter will be expressed in a similar pattern as the endogenous gene. The disruption usually mutates the endogenous gene."Although enhancer traps are widely used, certain gene traps were developed as an alteRNAtive to enhancer traps to capture open reading frame information. The identification of target genes using enhancer trapping was sometimes problematic because the site of reporter insertion could be as much as 100 kb from the target gene. This would require extensive characterization of the genomic insertion site to identify candidate target genes. gene trapping varies from enhancer trapping in that, instead of using a minimal promoter, gene trap vectors provide specific sequences that generate fusion RNA transcripts when inserted into a gene (See Fig. 1). This feature makes gene trapping (vs. enhancer trapping) especially advantageous in mammalian Cells that have complex genomic organization, including large Introns and small Exons, because the trapped gene can be identified by mRNA sequence. " From: [Durick K, et al. “Hunting with traps” genome research 9(11): 1019-1025. Nov. 1999] - nice review article.

Related Biology Tools

  • GenSmart™ Codon Optimization

    GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.

    Learn More

  • DNA Construct Design Tool

    GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.

    Learn More

  • Codon Frequency Tables

    This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.

    Learn More

Service and Products

Gene Synthesis

Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.

ORF cDNA Clones and Custom Clones

Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.

Plasmid DNA Preparation

High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.

Express Cloning & Subcloning

$49 and 2 day turnaround, choose from over 150 IP FREE vectors.

Mutation and Library Services

Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.

Oligo Synthesis

Bridge the gap between chemically synthesized oligos and bological applications.

If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: website@genscript.com

If your term is adopted, we will send 1,000 EzCoupon points to your GenScript account.