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gene cloning

The production of a Lineage of cells all of which contain one kind of DNA fragment of interest derived from a population of many kinds of DNA fragments. Operationally by: inserting (recombining) a population of DNA molecules, known to contain the DNA of interest, into a population of vector DNA molecules in such a way that each vector molecule contains only a single DNA molecule from the original population; transforming a population of Host cells with the vector DNA recombinants such that each Host cell takes up only one vector; growing single Host cells separately (cloning) by plating at low density to form a collection of separate colonies; screening the colonies (clones) formed for the presence of the DNA of interest.

Related Biology Tools

  • GenSmart™ Codon Optimization

    GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.

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  • DNA Construct Design Tool

    GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.

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  • Codon Frequency Tables

    This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.

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Service and Products

Cell Engineering

From lentivirus packaging to generating clonal cell lines, let us assist you on your assay cell line creation project.

Assay Cell Line Development

For Drug Discovery, Toxicity Testing and Basic Research.

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Lentiviruses are a powerful gene delivery vector that ensures long-term expression of your target transgene.

Research Grade AAV

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If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: website@genscript.com

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