Resources

List by Alphabet: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

cyclic amplification and selection of targets (CASTing)

Essentially identical to the selected and amplified (protein) binding site oligonucleotide (SAAB) and target detection assay (TDA) procedures; a procedure for identification of consensus sequences of DNA to which a protein, e.g. a transcription factor, may bind. A random polynucleotide sequence is synthesized flanked by two defined sequences that will serve as templates for PCR primers; the polynucleotides are exposed to the DNA-binding protein, any complex that is formed is separated from the unliganded polynucleotides (e.g. by gel shift assay, affinity chromatography, filter binding) and the polynucleotide of the complex is isolated and amplified by PCR; repeated recycling through the sequence of ligand formation, selection and amplification results in a preparation that is sufficiently pure to be cloned into bacteria for larger-scale production. A variant is systematic evolution of ligands by exponential enrichment (SELEX) for identification of RNA sequences, which begins with a mixture of polyribonucleotides and in each cycle produces DNA from the selected RNA-protein complex using reverse transcriptase, amplifies it by PCR, and then produces new RNA transcripts for the next round of selection. CASTing: Wright, W.E. and Funk, W.D. (1993) Trends Biochem. Sci. 18, 77-80; SAAB: Blackwell, T.K. and Weintraub, H.W. (1990) Science 250, 1104-1110; SELEX: Turek, C. and Gold, L. (1990) Science 249, 505-510; TDA: Thiesen, H.-J. and Bach, C. (1990) Nucleic Acids Res. 18, 3203-3209; Ouellette, M.M. and Wright, W.E. (1995) Curr. Opin. Biotechnol. 6, 65-72

Related Biology Tools

  • GenSmart™ Codon Optimization

    GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.

    Learn More

  • DNA Construct Design Tool

    GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.

    Learn More

  • Codon Frequency Tables

    This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.

    Learn More

Service and Products

Protein Expression

GenScript recombinant protein and rAb services provide high quality recombinant proteins and rAbs for a variety of downstream research applications.

Bacterial Expression

One-stop service from Sequence to Protein starting from $1600, 4 weeks.

Insect Expression

GenScript's BacuVance baculovirus expression system was developed by our in-house team of scientists for virus production and expression of recombinant proteins from baculovirus-infected insect cells.

Mammalian Transient Expression

Proprietary High Density (HD) expression system, enhance the protein yield up to 100 fold, achieve antibody titers up to 3 g/L.

Reagents for COVID-19 Research

Gram level, ready to ship RBD proteins, ACE2 assay cell lines and pseudovirus.

If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: website@genscript.com

If your term is adopted, we will send 1,000 EzCoupon points to your GenScript account.