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RNA ligase reverse transcription PCR (RL-RT-PCR)

A procedure for amplification of minute amounts of an unknown RNA (or DNA) sequence. The RNA fragment is ligated at both ends to single-stranded palindromic oligodeoxynucleotide that provides a site for subsequent hybridization with a second oligodeoxynucleotide. Reverse transcription uses as a primer the hybridized oligodeoxynucleotide, and then PCR amplification generates a family of polynucleotides that can be treated with a restriction endonuclease to produce large fragments of the cloned RNA and smaller fragments of linker oligonucleotides. Wahls, W.P. (1994) PCR Methods Appl. 3, 272-277

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Service and Products

Gene Synthesis

Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.

ORF cDNA Clones and Custom Clones

Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.

Plasmid DNA Preparation

High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.

Express Cloning & Subcloning

$49 and 2 day turnaround, choose from over 150 IP FREE vectors.

Mutation and Library Services

Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.

Oligo Synthesis

Bridge the gap between chemically synthesized oligos and bological applications.

If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: website@genscript.com

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