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dna constructs

DNA constructs, also known as recombinant DNA constructs or expression constructs, are artificially engineered segments of DNA that are created by combining different DNA sequences to achieve specific goals, such as expressing a particular gene or producing a desired protein. These constructs are widely used in molecular biology and genetic engineering for various research, biotechnological, and therapeutic purposes.

Key components of a DNA construct typically include:

1. Promoter: A promoter is a DNA sequence that initiates transcription, the process by which the DNA sequence is used as a template to synthesize RNA. It drives the expression of the gene of interest.

2. Gene of Interest (GOI): The gene of interest is the DNA sequence that encodes the protein or RNA molecule you want to study or produce. This could be a therapeutic protein, an enzyme, a fluorescent marker, or any other functional molecule.

3. Selectable Marker: In many cases, a selectable marker, such as a gene that confers resistance to an antibiotic, is included to identify cells that have successfully incorporated the DNA construct.

4. Polyadenylation Signal (PolyA): A polyadenylation signal sequence is added to the construct to signal the end of transcription and ensure proper processing of the mRNA.

5. Plasmid Backbone: The DNA construct is often inserted into a plasmid, a circular DNA molecule that serves as a carrier for the construct. The plasmid backbone contains essential elements for replication, stability, and propagation in bacterial or eukaryotic cells.

DNA constructs can be designed for various purposes:

• Gene Expression: DNA constructs are commonly used to express genes in different host organisms. By placing a gene of interest under the control of a promoter and inserting it into a plasmid, researchers can induce the production of a specific protein.

• Functional Studies: DNA constructs can be used to study the function of a gene or protein. Researchers might manipulate the gene or introduce mutations to observe the effects on cellular processes.

• Therapeutic Applications: DNA constructs are used in gene therapy to deliver therapeutic genes into cells, potentially correcting genetic defects or treating diseases.

• Molecular Cloning: DNA constructs are essential for the cloning and manipulation of DNA fragments, allowing researchers to amplify, sequence, or modify specific DNA sequences.

The design and assembly of DNA constructs involve molecular cloning techniques, such as PCR, restriction enzyme digestion, ligation, and transformation. The resulting DNA construct can be introduced into cells through methods like transfection, electroporation, or viral vectors, depending on the application and host organism.

GenScript related tools: DNA Construct Design Tool

Related Biology Tools

  • GenSmart™ Codon Optimization

    GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.

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  • DNA Construct Design Tool

    GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.

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  • Codon Frequency Tables

    This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.

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Service and Products

Gene Synthesis

Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.

ORF cDNA Clones and Custom Clones

Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.

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High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.

Express Cloning & Subcloning

$49 and 2 day turnaround, choose from over 150 IP FREE vectors.

Mutation and Library Services

Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.

Oligo Synthesis

Bridge the gap between chemically synthesized oligos and bological applications.

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