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Primer extension

This is a method used to figure out how far upstream from a fixed site the start of an mRNA is. For example, perhaps you have isolated a cDNA clone, but you don't think that the clone has all of the 5' untranslated region. To find out how much is missing, you would first sequence the part you have, and figure out which strand is coding strand (usually the coding strand will have a large open reading frame). Next, you ask the DNA Synthesis Facility to make an Oligonucleotide complementary to the 5'-most region of the coding strand (and thus complementary to the mRNA). This "primer" is hybridized to mRNA (say, a mixture of mRNA containing the one in which you are interested), and reverse transcriptase is added to copy the mRNA from the primer out to the 5' end. The size of the resulting DNA fragment shows how far away from the 5' end your primer is.

Related Biology Tools

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    GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.

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Service and Products

Gene Synthesis

Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.

ORF cDNA Clones and Custom Clones

Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.

Plasmid DNA Preparation

High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.

Express Cloning & Subcloning

$49 and 2 day turnaround, choose from over 150 IP FREE vectors.

Mutation and Library Services

Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.

Oligo Synthesis

Bridge the gap between chemically synthesized oligos and bological applications.

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