Resources » Technical Resource Centers » Peptide Technical Resources » Impact of TFA - A Review
In Fmoc-protected strategy, trifluoroacetic acid (TFA) is used to cleave the peptide from the resin support, and is added in the mobile phase for a better separation in purification. But TFA residues in the custom peptides may cause unpredictable fluctuations in the experimental data. The upper limit of TFA concentration varies in different researches. Check the blog to find the acceptable upper limit of TFA concentration on your experiment.
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