PCO063262 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	PCO063262
Entrez Gene ID	542166
Full Name	4-coumarate--CoA ligase 1
Synonyms	4CL,GRMZM2G075333
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
XM_020537578.2	XP_020393167.1	uncharacterized protein LOC542166 isoform X1
NM_001111788.2	NP_001105258.2	uncharacterized protein LOC542166
XM_020537578.1	XP_020393167.1	uncharacterized protein LOC542166 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.
Soderlund C, Descour A, Kudrna D, Bomhoff M, Boyd L, Currie J, Angelova A, Collura K, Wissotski M, Ashley E, Morrow D, Fernandes J, Walbot V, Yu Y
PLoS genetics5(11)e1000740(2009 Nov)

Anchoring 9,371 maize expressed sequence tagged unigenes to the bacterial artificial chromosome contig map by two-dimensional overgo hybridization.
Gardiner J, Schroeder S, Polacco ML, Sanchez-Villeda H, Fang Z, Morgante M, Landewe T, Fengler K, Useche F, Hanafey M, Tingey S, Chou H, Wing R, Soderlund C, Coe EH Jr
Plant physiology134(4)1317-26(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PCO063262 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCO063262 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OZa22446	NM_001111788.2 Latest version!	Zea mays 4-coumarate--CoA ligase 1 (LOC542166), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OZa61668	XM_020537578.2 Latest version!	Zea mays 4-coumarate--CoA ligase 1 (pco063262), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OZa61668	XM_020537578.1	Zea mays 4-coumarate--CoA ligase 1 (pco063262), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCO063262 ( NM_001111788.2 ) cDNA ORF clone, Zea mays -> NP_001105258.2 Zea mays 4-coumarate--CoA ligase 1 (LOC542166), mRNA.

Price & Availability↑

CloneID	OZa22446
Clone ID Related Accession (Same CDS sequence)	NM_001111788.2
Accession Version	NM_001111788.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551628800000
Organism	Zea mays
Product	uncharacterized protein LOC542166
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BT063202.1 and BT041522.1. On Jul 24, 2015 this sequence version replaced NM_001111788.1. ##Evidence-Data-START## Transcript exon combination :: BT041522.1, SRR3147047.9480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00780139, SAMN00780141 [ECO:0000348] ##Evidence-Data-END##

ATGGGTTCCG TAGACGCGGC GATCGCGGTG CCGGTGCCGG CGGCGGAGGA GAAGGCGGTG 
GAGGAGAAGG CGGTGGTGTT CCGGTCCAAG CTCCCCGACA TCGAGATCGA CAGCAGCATG 
GCGCTGCACA CCTACTGCTT CGGGAAGATG GGTGAGGTGG CGGAGCGGGC GTGCCTGGTC 
GACGGGCTGA CGGGCGCGTC GTACACGTAC GCGGAGGTGG AGTCCCTGTC CCGGCGCGCC 
GCGTCGGGGC TGCGCGCCAT GGGGGTGGGC AAGGGCGACG TGGTGATGAG CCTGCTCCGC 
AACTGCCCCG AGTTCGCCTT CACCTTCCTG GGCGCCGCCC GCCTGGGCGC CGCCACCACC 
ACGGCCAACC CGTTCTACAC CCCGCACGAG GTGCACCGCC AGGCGGAGGC GGCCGGCGCC 
CGGCTCATCG TGACCGAGGC CTGCGCCGTG GAGAAGGTGC GGGAGTTCGC GGCGGAGCGG 
GGCATCCCCG TGGTCACCGT CGACGGGCGC TTCGACGGCT GCGTGGAGTT CGCCGAGCTG 
ATCGCGGCCG AGGAGCTGGA GGCCGACGCC GACATCCACC CCGACGACGT CGTCGCGCTG 
CCCTACTCCT CCGGCACCAC CGGGCTGCCC AAGGGCGTCA TGCTCACCCA CCGCAGCCTC 
ATCACCAGCG TCGCGCAGCA GGTTGATGGC GAGAACCCGA ACCTGTACTT CCGCAAGGAC 
GACGTGGTGC TGTGCCTGCT GCCGCTGTTC CACATCTACT CGCTGAACTC GGTGCTGCTG 
GCCGGCCTGC GCGCGGGCTC CACCATCGTG ATCATGCGCA AGTTCGACCT GGGCGCGCTG 
GTGGACCTGG TGCGCAGGTA CGTGATCACC ATCGCGCCCT TCGTGCCGCC CATCGTGGTG 
GAGATCGCCA AGAGCCCCCG CGTGACCGCC GGCGACCTCG CGTCCATCCG CATGGTCATG 
TCCGGCGCCG CGCCCATGGG CAAGGAGCTC CAGGACGCCT TCATGGCCAA GATTCCCAAT 
GCCGTGCTCG GGCAGGGGTA CGGGATGACG GAGGCAGGCC CCGTGCTGGC GATGTGCCTG 
GCCTTCGCCA AGGAGCCGTA CCCGGTCAAG TCCGGGTCGT GCGGCACCGT GGTGCGGAAC 
GCGGAGCTGA AGATCGTCGA CCCCGACACC GGCGCCGCCC TCGGCCGGAA CCAGCCCGGC 
GAGATCTGCA TCCGCGGGGA GCAGATCATG AAAGGTTACC TGAACGACCC CGAGTCGACG 
AAGAACACCA TCGACAAGGA CGGCTGGCTG CACACCGGAG ACATCGGCTA CGTGGACGAC 
GACGACGAGA TCTTCATCGT CGACAGGCTC AAGGAGATCA TCAAGTACAA GGGCTTCCAG 
GTGCCGCCGG CGGAGCTGGA GGCGCTCCTC ATCACGCACC CGGAGATCAA GGACGCCGCC 
GTCGTATCAA TGAACGATGA CCTTGCTGGT GAAATCCCGG TCGCCTTCAT CGTGCGGACC 
GAAGGTTCTC AAGTCACCGA GGATGAGATC AAGCAATTCG TCGCCAAGGA GGTGGTTTTC 
TACAAGAAGA TCCACAAGGT CTTCTTCACC GAATCCATCC CCAAGAACCC GTCGGGCAAG 
ATCCTGAGGA AGGACTTGAG AGCCAGGCTC GCCGCCGGTG TTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001105258.2
CDS	132..1799
Translation	MGSVDAAIAVPVPAAEEKAVEEKAVVFRSKLPDIEIDSSMALHTYCFGKMGEVAERACLVDGLTGASYTYAEVESLSRRAASGLRAMGVGKGDVVMSLLRNCPEFAFTFLGAARLGAATTTANPFYTPHEVHRQAEAAGARLIVTEACAVEKVREFAAERGIPVVTVDGRFDGCVEFAELIAAEELEADADIHPDDVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGENPNLYFRKDDVVLCLLPLFHIYSLNSVLLAGLRAGSTIVIMRKFDLGALVDLVRRYVITIAPFVPPIVVEIAKSPRVTAGDLASIRMVMSGAAPMGKELQDAFMAKIPNAVLGQGYGMTEAGPVLAMCLAFAKEPYPVKSGSCGTVVRNAELKIVDPDTGAALGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARLAAGVH

Target ORF information:

RefSeq Version	NM_001111788.2
Organism	Zea mays
Definition	Zea mays 4-coumarate--CoA ligase 1 (LOC542166), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111788.2

ORF Insert Sequence:

ATGGGTTCCG TAGACGCGGC GATCGCGGTG CCGGTGCCGG CGGCGGAGGA GAAGGCGGTG 
GAGGAGAAGG CGGTGGTGTT CCGGTCCAAG CTCCCCGACA TCGAGATCGA CAGCAGCATG 
GCGCTGCACA CCTACTGCTT CGGGAAGATG GGTGAGGTGG CGGAGCGGGC GTGCCTGGTC 
GACGGGCTGA CGGGCGCGTC GTACACGTAC GCGGAGGTGG AGTCCCTGTC CCGGCGCGCC 
GCGTCGGGGC TGCGCGCCAT GGGGGTGGGC AAGGGCGACG TGGTGATGAG CCTGCTCCGC 
AACTGCCCCG AGTTCGCCTT CACCTTCCTG GGCGCCGCCC GCCTGGGCGC CGCCACCACC 
ACGGCCAACC CGTTCTACAC CCCGCACGAG GTGCACCGCC AGGCGGAGGC GGCCGGCGCC 
CGGCTCATCG TGACCGAGGC CTGCGCCGTG GAGAAGGTGC GGGAGTTCGC GGCGGAGCGG 
GGCATCCCCG TGGTCACCGT CGACGGGCGC TTCGACGGCT GCGTGGAGTT CGCCGAGCTG 
ATCGCGGCCG AGGAGCTGGA GGCCGACGCC GACATCCACC CCGACGACGT CGTCGCGCTG 
CCCTACTCCT CCGGCACCAC CGGGCTGCCC AAGGGCGTCA TGCTCACCCA CCGCAGCCTC 
ATCACCAGCG TCGCGCAGCA GGTTGATGGC GAGAACCCGA ACCTGTACTT CCGCAAGGAC 
GACGTGGTGC TGTGCCTGCT GCCGCTGTTC CACATCTACT CGCTGAACTC GGTGCTGCTG 
GCCGGCCTGC GCGCGGGCTC CACCATCGTG ATCATGCGCA AGTTCGACCT GGGCGCGCTG 
GTGGACCTGG TGCGCAGGTA CGTGATCACC ATCGCGCCCT TCGTGCCGCC CATCGTGGTG 
GAGATCGCCA AGAGCCCCCG CGTGACCGCC GGCGACCTCG CGTCCATCCG CATGGTCATG 
TCCGGCGCCG CGCCCATGGG CAAGGAGCTC CAGGACGCCT TCATGGCCAA GATTCCCAAT 
GCCGTGCTCG GGCAGGGGTA CGGGATGACG GAGGCAGGCC CCGTGCTGGC GATGTGCCTG 
GCCTTCGCCA AGGAGCCGTA CCCGGTCAAG TCCGGGTCGT GCGGCACCGT GGTGCGGAAC 
GCGGAGCTGA AGATCGTCGA CCCCGACACC GGCGCCGCCC TCGGCCGGAA CCAGCCCGGC 
GAGATCTGCA TCCGCGGGGA GCAGATCATG AAAGGTTACC TGAACGACCC CGAGTCGACG 
AAGAACACCA TCGACAAGGA CGGCTGGCTG CACACCGGAG ACATCGGCTA CGTGGACGAC 
GACGACGAGA TCTTCATCGT CGACAGGCTC AAGGAGATCA TCAAGTACAA GGGCTTCCAG 
GTGCCGCCGG CGGAGCTGGA GGCGCTCCTC ATCACGCACC CGGAGATCAA GGACGCCGCC 
GTCGTATCAA TGAACGATGA CCTTGCTGGT GAAATCCCGG TCGCCTTCAT CGTGCGGACC 
GAAGGTTCTC AAGTCACCGA GGATGAGATC AAGCAATTCG TCGCCAAGGA GGTGGTTTTC 
TACAAGAAGA TCCACAAGGT CTTCTTCACC GAATCCATCC CCAAGAACCC GTCGGGCAAG 
ATCCTGAGGA AGGACTTGAG AGCCAGGCTC GCCGCCGGTG TTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCO063262 ( XM_020537578.2 ) cDNA ORF clone, Zea mays -> XP_020393167.1 Zea mays 4-coumarate--CoA ligase 1 (pco063262), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OZa61668
Clone ID Related Accession (Same CDS sequence)	XM_020537578.2 , XM_020537578.1
Accession Version	XM_020537578.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	uncharacterized protein LOC542166 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024463.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2017 this sequence version replaced XM_020537578.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCG TAGACGCGGC GATCGCGGTG CCGGTGCCGG CGGCGGAGGA GAAGGCGGTG 
GAGGAGAAGG CGGTGGTGTT CCGGTCCAAG CTCCCCGACA TCGAGATCGA CAGCAGCATG 
GCGCTGCACA CCTACTGCTT CGGGAAGATG GGTGAGGTGG CGGAGCGGGC GTGCCTGGTC 
GACGGGCTGA CGGGCGCGTC GTACACGTAC GCGGAGGTGG AGTCCCTGTC CCGGCGCGCC 
GCGTCGGGGC TGCGCGCCAT GGGGGTGGGC AAGGGCGACG TGGTGATGAG CCTGCTCCGC 
AACTGCCCCG AGTTCGCCTT CACCTTCCTG GGCGCCGCCC GCCTGGGCGC CGCCACCACC 
ACGGCCAACC CGTTCTACAC CCCGCACGAG GTGCACCGCC AGGCGGAGGC GGCCGGCGCC 
CGGCTCATCG TGACCGAGGC CTGCGCCGTG GAGAAGGTGC GGGAGTTCGC GGCGGAGCGG 
GGCATCCCCG TGGTCACCGT CGACGGGCGC TTCGACGGCT GCGTGGAGTT CGCCGAGCTG 
ATCGCGGCCG AGGAGCTGGA GGCCGACGCC GACATCCACC CCGACGACGT CGTCGCGCTG 
CCCTACTCCT CCGGCACCAC CGGGCTGCCC AAGGGCGTCA TGCTCACCCA CCGCAGCCTC 
ATCACCAGCG TCGCGCAGCA GGTTGATGGC GAGAACCCGA ACCTGTACTT CCGCAAGGAC 
GACGTGGTGC TGTGCCTGCT GCCGCTGTTC CACATCTACT CGCTGAACTC GGTGCTGCTG 
GCCGGCCTGC GCGCGGGCTC CACCATCGTG ATCATGCGCA AGTTCGACCT GGGCGCGCTG 
GTGGACCTGG TGCGCAGGTA CGTGATCACC ATCGCGCCCT TCGTGCCGCC CATCGTGGTG 
GAGATCGCCA AGAGCCCCCG CGTGACCGCC GGCGACCTCG CGTCCATCCG CATGGTCATG 
TCCGGCGCCG CGCCCATGGG CAAGGAGCTC CAGGACGCCT TCATGGCCAA GATTCCCAAT 
GCCGTGCTCG GGCAGGTACG TCCGTCGTCG TCGTCCGAAG CTTTTTCGGT TTTCCTGATG 
ACACGCGTTC CATTCCGTTC CCATTCCGAC CAATGCAATG CAATGCAACA GGGGTACGGG 
ATGACGGAGG CAGGCCCCGT GCTGGCGATG TGCCTGGCCT TCGCCAAGGA GCCGTACCCG 
GTCAAGTCCG GGTCGTGCGG CACCGTGGTG CGGAACGCGG AGCTGAAGAT CGTCGACCCC 
GACACCGGCG CCGCCCTCGG CCGGAACCAG CCCGGCGAGA TCTGCATCCG CGGGGAGCAG 
ATCATGAAAG GTTACCTGAA CGACCCCGAG TCGACGAAGA ACACCATCGA CAAGGACGGC 
TGGCTGCACA CCGGAGACAT CGGCTACGTG GACGACGACG ACGAGATCTT CATCGTCGAC 
AGGCTCAAGG AGATCATCAA GTACAAGGGC TTCCAGGTGC CGCCGGCGGA GCTGGAGGCG 
CTCCTCATCA CGCACCCGGA GATCAAGGAC GCCGCCGTCG TATCAATGAA CGATGACCTT 
GCTGGTGAAA TCCCGGTCGC CTTCATCGTG CGGACCGAAG GTTCTCAAGT CACCGAGGAT 
GAGATCAAGC AATTCGTCGC CAAGGAGGTG GTTTTCTACA AGAAGATCCA CAAGGTCTTC 
TTCACCGAAT CCATCCCCAA GAACCCGTCG GGCAAGATCC TGAGGAAGGA CTTGAGAGCC 
AGGCTCGCCG CCGGTGTTCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020393167.1
CDS	164..1927
Translation	MGSVDAAIAVPVPAAEEKAVEEKAVVFRSKLPDIEIDSSMALHTYCFGKMGEVAERACLVDGLTGASYTYAEVESLSRRAASGLRAMGVGKGDVVMSLLRNCPEFAFTFLGAARLGAATTTANPFYTPHEVHRQAEAAGARLIVTEACAVEKVREFAAERGIPVVTVDGRFDGCVEFAELIAAEELEADADIHPDDVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGENPNLYFRKDDVVLCLLPLFHIYSLNSVLLAGLRAGSTIVIMRKFDLGALVDLVRRYVITIAPFVPPIVVEIAKSPRVTAGDLASIRMVMSGAAPMGKELQDAFMAKIPNAVLGQVRPSSSSEAFSVFLMTRVPFRSHSDQCNAMQQGYGMTEAGPVLAMCLAFAKEPYPVKSGSCGTVVRNAELKIVDPDTGAALGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARLAAGVH

Target ORF information:

RefSeq Version	XM_020537578.2
Organism	Zea mays
Definition	Zea mays 4-coumarate--CoA ligase 1 (pco063262), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020537578.2

ORF Insert Sequence:

ATGGGTTCCG TAGACGCGGC GATCGCGGTG CCGGTGCCGG CGGCGGAGGA GAAGGCGGTG 
GAGGAGAAGG CGGTGGTGTT CCGGTCCAAG CTCCCCGACA TCGAGATCGA CAGCAGCATG 
GCGCTGCACA CCTACTGCTT CGGGAAGATG GGTGAGGTGG CGGAGCGGGC GTGCCTGGTC 
GACGGGCTGA CGGGCGCGTC GTACACGTAC GCGGAGGTGG AGTCCCTGTC CCGGCGCGCC 
GCGTCGGGGC TGCGCGCCAT GGGGGTGGGC AAGGGCGACG TGGTGATGAG CCTGCTCCGC 
AACTGCCCCG AGTTCGCCTT CACCTTCCTG GGCGCCGCCC GCCTGGGCGC CGCCACCACC 
ACGGCCAACC CGTTCTACAC CCCGCACGAG GTGCACCGCC AGGCGGAGGC GGCCGGCGCC 
CGGCTCATCG TGACCGAGGC CTGCGCCGTG GAGAAGGTGC GGGAGTTCGC GGCGGAGCGG 
GGCATCCCCG TGGTCACCGT CGACGGGCGC TTCGACGGCT GCGTGGAGTT CGCCGAGCTG 
ATCGCGGCCG AGGAGCTGGA GGCCGACGCC GACATCCACC CCGACGACGT CGTCGCGCTG 
CCCTACTCCT CCGGCACCAC CGGGCTGCCC AAGGGCGTCA TGCTCACCCA CCGCAGCCTC 
ATCACCAGCG TCGCGCAGCA GGTTGATGGC GAGAACCCGA ACCTGTACTT CCGCAAGGAC 
GACGTGGTGC TGTGCCTGCT GCCGCTGTTC CACATCTACT CGCTGAACTC GGTGCTGCTG 
GCCGGCCTGC GCGCGGGCTC CACCATCGTG ATCATGCGCA AGTTCGACCT GGGCGCGCTG 
GTGGACCTGG TGCGCAGGTA CGTGATCACC ATCGCGCCCT TCGTGCCGCC CATCGTGGTG 
GAGATCGCCA AGAGCCCCCG CGTGACCGCC GGCGACCTCG CGTCCATCCG CATGGTCATG 
TCCGGCGCCG CGCCCATGGG CAAGGAGCTC CAGGACGCCT TCATGGCCAA GATTCCCAAT 
GCCGTGCTCG GGCAGGTACG TCCGTCGTCG TCGTCCGAAG CTTTTTCGGT TTTCCTGATG 
ACACGCGTTC CATTCCGTTC CCATTCCGAC CAATGCAATG CAATGCAACA GGGGTACGGG 
ATGACGGAGG CAGGCCCCGT GCTGGCGATG TGCCTGGCCT TCGCCAAGGA GCCGTACCCG 
GTCAAGTCCG GGTCGTGCGG CACCGTGGTG CGGAACGCGG AGCTGAAGAT CGTCGACCCC 
GACACCGGCG CCGCCCTCGG CCGGAACCAG CCCGGCGAGA TCTGCATCCG CGGGGAGCAG 
ATCATGAAAG GTTACCTGAA CGACCCCGAG TCGACGAAGA ACACCATCGA CAAGGACGGC 
TGGCTGCACA CCGGAGACAT CGGCTACGTG GACGACGACG ACGAGATCTT CATCGTCGAC 
AGGCTCAAGG AGATCATCAA GTACAAGGGC TTCCAGGTGC CGCCGGCGGA GCTGGAGGCG 
CTCCTCATCA CGCACCCGGA GATCAAGGAC GCCGCCGTCG TATCAATGAA CGATGACCTT 
GCTGGTGAAA TCCCGGTCGC CTTCATCGTG CGGACCGAAG GTTCTCAAGT CACCGAGGAT 
GAGATCAAGC AATTCGTCGC CAAGGAGGTG GTTTTCTACA AGAAGATCCA CAAGGTCTTC 
TTCACCGAAT CCATCCCCAA GAACCCGTCG GGCAAGATCC TGAGGAAGGA CTTGAGAGCC 
AGGCTCGCCG CCGGTGTTCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCO063262 ( XM_020537578.1 ) cDNA ORF clone, Zea mays -> XP_020393167.1 Zea mays 4-coumarate--CoA ligase 1 (pco063262), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OZa61668
Clone ID Related Accession (Same CDS sequence)	XM_020537578.2 , XM_020537578.1
Accession Version	XM_020537578.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1489852800000
Organism	Zea mays
Product	uncharacterized protein LOC542166 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024463.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCG TAGACGCGGC GATCGCGGTG CCGGTGCCGG CGGCGGAGGA GAAGGCGGTG 
GAGGAGAAGG CGGTGGTGTT CCGGTCCAAG CTCCCCGACA TCGAGATCGA CAGCAGCATG 
GCGCTGCACA CCTACTGCTT CGGGAAGATG GGTGAGGTGG CGGAGCGGGC GTGCCTGGTC 
GACGGGCTGA CGGGCGCGTC GTACACGTAC GCGGAGGTGG AGTCCCTGTC CCGGCGCGCC 
GCGTCGGGGC TGCGCGCCAT GGGGGTGGGC AAGGGCGACG TGGTGATGAG CCTGCTCCGC 
AACTGCCCCG AGTTCGCCTT CACCTTCCTG GGCGCCGCCC GCCTGGGCGC CGCCACCACC 
ACGGCCAACC CGTTCTACAC CCCGCACGAG GTGCACCGCC AGGCGGAGGC GGCCGGCGCC 
CGGCTCATCG TGACCGAGGC CTGCGCCGTG GAGAAGGTGC GGGAGTTCGC GGCGGAGCGG 
GGCATCCCCG TGGTCACCGT CGACGGGCGC TTCGACGGCT GCGTGGAGTT CGCCGAGCTG 
ATCGCGGCCG AGGAGCTGGA GGCCGACGCC GACATCCACC CCGACGACGT CGTCGCGCTG 
CCCTACTCCT CCGGCACCAC CGGGCTGCCC AAGGGCGTCA TGCTCACCCA CCGCAGCCTC 
ATCACCAGCG TCGCGCAGCA GGTTGATGGC GAGAACCCGA ACCTGTACTT CCGCAAGGAC 
GACGTGGTGC TGTGCCTGCT GCCGCTGTTC CACATCTACT CGCTGAACTC GGTGCTGCTG 
GCCGGCCTGC GCGCGGGCTC CACCATCGTG ATCATGCGCA AGTTCGACCT GGGCGCGCTG 
GTGGACCTGG TGCGCAGGTA CGTGATCACC ATCGCGCCCT TCGTGCCGCC CATCGTGGTG 
GAGATCGCCA AGAGCCCCCG CGTGACCGCC GGCGACCTCG CGTCCATCCG CATGGTCATG 
TCCGGCGCCG CGCCCATGGG CAAGGAGCTC CAGGACGCCT TCATGGCCAA GATTCCCAAT 
GCCGTGCTCG GGCAGGTACG TCCGTCGTCG TCGTCCGAAG CTTTTTCGGT TTTCCTGATG 
ACACGCGTTC CATTCCGTTC CCATTCCGAC CAATGCAATG CAATGCAACA GGGGTACGGG 
ATGACGGAGG CAGGCCCCGT GCTGGCGATG TGCCTGGCCT TCGCCAAGGA GCCGTACCCG 
GTCAAGTCCG GGTCGTGCGG CACCGTGGTG CGGAACGCGG AGCTGAAGAT CGTCGACCCC 
GACACCGGCG CCGCCCTCGG CCGGAACCAG CCCGGCGAGA TCTGCATCCG CGGGGAGCAG 
ATCATGAAAG GTTACCTGAA CGACCCCGAG TCGACGAAGA ACACCATCGA CAAGGACGGC 
TGGCTGCACA CCGGAGACAT CGGCTACGTG GACGACGACG ACGAGATCTT CATCGTCGAC 
AGGCTCAAGG AGATCATCAA GTACAAGGGC TTCCAGGTGC CGCCGGCGGA GCTGGAGGCG 
CTCCTCATCA CGCACCCGGA GATCAAGGAC GCCGCCGTCG TATCAATGAA CGATGACCTT 
GCTGGTGAAA TCCCGGTCGC CTTCATCGTG CGGACCGAAG GTTCTCAAGT CACCGAGGAT 
GAGATCAAGC AATTCGTCGC CAAGGAGGTG GTTTTCTACA AGAAGATCCA CAAGGTCTTC 
TTCACCGAAT CCATCCCCAA GAACCCGTCG GGCAAGATCC TGAGGAAGGA CTTGAGAGCC 
AGGCTCGCCG CCGGTGTTCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020393167.1
CDS	160..1923
Translation	MGSVDAAIAVPVPAAEEKAVEEKAVVFRSKLPDIEIDSSMALHTYCFGKMGEVAERACLVDGLTGASYTYAEVESLSRRAASGLRAMGVGKGDVVMSLLRNCPEFAFTFLGAARLGAATTTANPFYTPHEVHRQAEAAGARLIVTEACAVEKVREFAAERGIPVVTVDGRFDGCVEFAELIAAEELEADADIHPDDVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGENPNLYFRKDDVVLCLLPLFHIYSLNSVLLAGLRAGSTIVIMRKFDLGALVDLVRRYVITIAPFVPPIVVEIAKSPRVTAGDLASIRMVMSGAAPMGKELQDAFMAKIPNAVLGQVRPSSSSEAFSVFLMTRVPFRSHSDQCNAMQQGYGMTEAGPVLAMCLAFAKEPYPVKSGSCGTVVRNAELKIVDPDTGAALGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARLAAGVH

Target ORF information:

RefSeq Version	XM_020537578.1
Organism	Zea mays
Definition	Zea mays 4-coumarate--CoA ligase 1 (pco063262), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020537578.1

ORF Insert Sequence:

ATGGGTTCCG TAGACGCGGC GATCGCGGTG CCGGTGCCGG CGGCGGAGGA GAAGGCGGTG 
GAGGAGAAGG CGGTGGTGTT CCGGTCCAAG CTCCCCGACA TCGAGATCGA CAGCAGCATG 
GCGCTGCACA CCTACTGCTT CGGGAAGATG GGTGAGGTGG CGGAGCGGGC GTGCCTGGTC 
GACGGGCTGA CGGGCGCGTC GTACACGTAC GCGGAGGTGG AGTCCCTGTC CCGGCGCGCC 
GCGTCGGGGC TGCGCGCCAT GGGGGTGGGC AAGGGCGACG TGGTGATGAG CCTGCTCCGC 
AACTGCCCCG AGTTCGCCTT CACCTTCCTG GGCGCCGCCC GCCTGGGCGC CGCCACCACC 
ACGGCCAACC CGTTCTACAC CCCGCACGAG GTGCACCGCC AGGCGGAGGC GGCCGGCGCC 
CGGCTCATCG TGACCGAGGC CTGCGCCGTG GAGAAGGTGC GGGAGTTCGC GGCGGAGCGG 
GGCATCCCCG TGGTCACCGT CGACGGGCGC TTCGACGGCT GCGTGGAGTT CGCCGAGCTG 
ATCGCGGCCG AGGAGCTGGA GGCCGACGCC GACATCCACC CCGACGACGT CGTCGCGCTG 
CCCTACTCCT CCGGCACCAC CGGGCTGCCC AAGGGCGTCA TGCTCACCCA CCGCAGCCTC 
ATCACCAGCG TCGCGCAGCA GGTTGATGGC GAGAACCCGA ACCTGTACTT CCGCAAGGAC 
GACGTGGTGC TGTGCCTGCT GCCGCTGTTC CACATCTACT CGCTGAACTC GGTGCTGCTG 
GCCGGCCTGC GCGCGGGCTC CACCATCGTG ATCATGCGCA AGTTCGACCT GGGCGCGCTG 
GTGGACCTGG TGCGCAGGTA CGTGATCACC ATCGCGCCCT TCGTGCCGCC CATCGTGGTG 
GAGATCGCCA AGAGCCCCCG CGTGACCGCC GGCGACCTCG CGTCCATCCG CATGGTCATG 
TCCGGCGCCG CGCCCATGGG CAAGGAGCTC CAGGACGCCT TCATGGCCAA GATTCCCAAT 
GCCGTGCTCG GGCAGGTACG TCCGTCGTCG TCGTCCGAAG CTTTTTCGGT TTTCCTGATG 
ACACGCGTTC CATTCCGTTC CCATTCCGAC CAATGCAATG CAATGCAACA GGGGTACGGG 
ATGACGGAGG CAGGCCCCGT GCTGGCGATG TGCCTGGCCT TCGCCAAGGA GCCGTACCCG 
GTCAAGTCCG GGTCGTGCGG CACCGTGGTG CGGAACGCGG AGCTGAAGAT CGTCGACCCC 
GACACCGGCG CCGCCCTCGG CCGGAACCAG CCCGGCGAGA TCTGCATCCG CGGGGAGCAG 
ATCATGAAAG GTTACCTGAA CGACCCCGAG TCGACGAAGA ACACCATCGA CAAGGACGGC 
TGGCTGCACA CCGGAGACAT CGGCTACGTG GACGACGACG ACGAGATCTT CATCGTCGAC 
AGGCTCAAGG AGATCATCAA GTACAAGGGC TTCCAGGTGC CGCCGGCGGA GCTGGAGGCG 
CTCCTCATCA CGCACCCGGA GATCAAGGAC GCCGCCGTCG TATCAATGAA CGATGACCTT 
GCTGGTGAAA TCCCGGTCGC CTTCATCGTG CGGACCGAAG GTTCTCAAGT CACCGAGGAT 
GAGATCAAGC AATTCGTCGC CAAGGAGGTG GTTTTCTACA AGAAGATCCA CAAGGTCTTC 
TTCACCGAAT CCATCCCCAA GAACCCGTCG GGCAAGATCC TGAGGAAGGA CTTGAGAGCC 
AGGCTCGCCG CCGGTGTTCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.
PLoS genetics5(11)e1000740(2009 Nov)
Soderlund C,Descour A,Kudrna D,Bomhoff M,Boyd L,Currie J,Angelova A,Collura K,Wissotski M,Ashley E,Morrow D,Fernandes J,Walbot V,Yu Y

Anchoring 9,371 maize expressed sequence tagged unigenes to the bacterial artificial chromosome contig map by two-dimensional overgo hybridization.
Plant physiology134(4)1317-26(2004 Apr)
Gardiner J,Schroeder S,Polacco ML,Sanchez-Villeda H,Fang Z,Morgante M,Landewe T,Fengler K,Useche F,Hanafey M,Tingey S,Chou H,Wing R,Soderlund C,Coe EH Jr

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PCO063262 cDNA ORF clone, Zea mays

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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