AY109804 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	AY109804
Entrez Gene ID	100381411
Full Name	Alpha-glucan water dikinase 1 chloroplastic
Synonyms	GRMZM2G412611
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
XM_008649791.2	XP_008648013.1	alpha-glucan water dikinase 1 chloroplastic isoform X1
XM_020539039.1	XP_020394628.1	alpha-glucan water dikinase 1 chloroplastic isoform X1
NM_001174252.1	NP_001167723.1	Alpha-glucan water dikinase 1 chloroplastic
XM_020539038.1	XP_020394627.1	alpha-glucan water dikinase 1 chloroplastic isoform X1
NM_001361424.1	NP_001348353.1	Alpha-glucan water dikinase 1 chloroplastic
XM_020539039.2	XP_020394628.1	alpha-glucan water dikinase 1 chloroplastic isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.
Soderlund C, Descour A, Kudrna D, Bomhoff M, Boyd L, Currie J, Angelova A, Collura K, Wissotski M, Ashley E, Morrow D, Fernandes J, Walbot V, Yu Y
PLoS genetics5(11)e1000740(2009 Nov)

Anchoring 9,371 maize expressed sequence tagged unigenes to the bacterial artificial chromosome contig map by two-dimensional overgo hybridization.
Gardiner J, Schroeder S, Polacco ML, Sanchez-Villeda H, Fang Z, Morgante M, Landewe T, Fengler K, Useche F, Hanafey M, Tingey S, Chou H, Wing R, Soderlund C, Coe EH Jr
Plant physiology134(4)1317-26(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AY109804 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AY109804 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OZa10461	NM_001174252.1 Latest version!	Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OZa29821	NM_001361424.1 Latest version!	Zea mays Alpha-glucan water dikinase 1 chloroplastic (LOC100381411), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OZa29821	XM_008649791.2 Latest version!	Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OZa29821	XM_020539038.1 Latest version!	Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OZa29821	XM_020539039.2 Latest version!	Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OZa29821	XM_020539039.1	Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AY109804 ( NM_001174252.1 ) cDNA ORF clone, Zea mays -> NP_001167723.1 Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), mRNA.

Price & Availability↑

CloneID	OZa10461
Clone ID Related Accession (Same CDS sequence)	NM_001174252.1
Accession Version	NM_001174252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514390400000
Organism	Zea mays
Product	Alpha-glucan water dikinase 1 chloroplastic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT061194.1. ##Evidence-Data-START## Transcript exon combination :: BT061194.1, DT939285.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00780084, SAMN00780085 [ECO:0000348] ##Evidence-Data-END##

ATGCAATTGG TTAATGAGCT GAAGGAGAGG ATGCTAGGCT CTGGAATGCC CTGGCCTGGT 
GATGAAGGAG ACAAGCGTTG GGAGCAAGCA TGGATGGCTA TTAAAAAGGT TTGGGCATCA 
AAATGGAACG AAAGAGCATA TTTTAGCACA CGCAAGGTGA AACTTGATCA TGAGTACCTT 
TCGATGGCTG TTCTCGTGCA AGAAGTTGTG AATGCAGATT ATGCTTTTGT CATTCATACC 
ACAAACCCAT CGTCTGGAGA TTCTTCTGAG ATATATGCTG AAGTGGTGAA AGGGCTTGGC 
GAGACCCTCG TGGGAGCCTA TCCTGGTCGT GCTATGAGCT TTGTTTGCAA AAAAGATGAC 
CTTGACTCTC CCAAGTTACT TGGTTACCCA AGCAAGCCAA TTGGTCTCTT CATAAGGCAA 
TCAATCATCT TCCGTTCCGA CTCCAACGGT GAGGACCTGG AAGGTTATGC TGGAGCAGGA 
TTATATGATA GTGTACCGAT GGATGAGGAG GATGAGGTTG TACTTGATTA TACAACTGAC 
CCTCTTATAG TAGACCGTGG ATTCCGAAGC TCAATCCTCT CAAGCATAGC ACGGGCTGGC 
CATGCCATCG AGGAGCTATA TGGTTCTCCT CAGGACGTCG AGGGAGTAGT GAAGGATGGA 
AAAATCTATG TAGTCCAGAC AAGACCACAG ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001167723.1
CDS	142..837
Translation	MQLVNELKERMLGSGMPWPGDEGDKRWEQAWMAIKKVWASKWNERAYFSTRKVKLDHEYLSMAVLVQEVVNADYAFVIHTTNPSSGDSSEIYAEVVKGLGETLVGAYPGRAMSFVCKKDDLDSPKLLGYPSKPIGLFIRQSIIFRSDSNGEDLEGYAGAGLYDSVPMDEEDEVVLDYTTDPLIVDRGFRSSILSSIARAGHAIEELYGSPQDVEGVVKDGKIYVVQTRPQM

Target ORF information:

RefSeq Version	NM_001174252.1
Organism	Zea mays
Definition	Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001174252.1

ORF Insert Sequence:

ATGCAATTGG TTAATGAGCT GAAGGAGAGG ATGCTAGGCT CTGGAATGCC CTGGCCTGGT 
GATGAAGGAG ACAAGCGTTG GGAGCAAGCA TGGATGGCTA TTAAAAAGGT TTGGGCATCA 
AAATGGAACG AAAGAGCATA TTTTAGCACA CGCAAGGTGA AACTTGATCA TGAGTACCTT 
TCGATGGCTG TTCTCGTGCA AGAAGTTGTG AATGCAGATT ATGCTTTTGT CATTCATACC 
ACAAACCCAT CGTCTGGAGA TTCTTCTGAG ATATATGCTG AAGTGGTGAA AGGGCTTGGC 
GAGACCCTCG TGGGAGCCTA TCCTGGTCGT GCTATGAGCT TTGTTTGCAA AAAAGATGAC 
CTTGACTCTC CCAAGTTACT TGGTTACCCA AGCAAGCCAA TTGGTCTCTT CATAAGGCAA 
TCAATCATCT TCCGTTCCGA CTCCAACGGT GAGGACCTGG AAGGTTATGC TGGAGCAGGA 
TTATATGATA GTGTACCGAT GGATGAGGAG GATGAGGTTG TACTTGATTA TACAACTGAC 
CCTCTTATAG TAGACCGTGG ATTCCGAAGC TCAATCCTCT CAAGCATAGC ACGGGCTGGC 
CATGCCATCG AGGAGCTATA TGGTTCTCCT CAGGACGTCG AGGGAGTAGT GAAGGATGGA 
AAAATCTATG TAGTCCAGAC AAGACCACAG ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AY109804 ( NM_001361424.1 ) cDNA ORF clone, Zea mays -> NP_001348353.1 Zea mays Alpha-glucan water dikinase 1 chloroplastic (LOC100381411), mRNA.

Price & Availability↑

CloneID	OZa29821
Clone ID Related Accession (Same CDS sequence)	XM_008649791.2 , XM_020539039.1 , XM_020539038.1 , NM_001361424.1 , XM_020539039.2
Accession Version	NM_001361424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1557676800000
Organism	Zea mays
Product	Alpha-glucan water dikinase 1 chloroplastic
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from LPUQ01002335.1, CD651175.1 and BT061194.1. On Mar 28, 2018 this sequence version replaced XM_008649791.2. ##Evidence-Data-START## Transcript exon combination :: SRR3147031.15396.1, SRR3147031.30054.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00780084, SAMN00780085 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGGAT TCAGTGCCGC GGCCAACGCA GCGGCGGCTG AGCGGTGCGC GCTCGCGTTC 
CGCGCACGGC CCGCGGCCTC CTCGCCAGCG AAGCGGCAGC AGCAGCCGCA GCCAGCGTCC 
CTCCGACGCA GCGGGGGCCA GCGCCGCCCC ACGACGCTCT CCGCCTCTAG CCGCGGCCCC 
GTCGTGCCGC GCGCCGTCGC CACGTCCGCG GACCGCGCGT CCCCCGACCT TATCGGAAAG 
TTCACGCTGG ATTCCAACTC CGAGCTCCAG GTCGCAGTGA ACCCAGCGCC GCAGGGTTTG 
GTGTCAGAGA TTAGCCTGGA GGTGACCAAC ACAAGCGGTT CCCTGATTTT GCATTGGGGA 
GCCCTTCGCC CGGACAAGAG AGATTGGATC CTCCCGTCCA GAAAACCTGA TGGAACGACA 
GTGTACAAGA ACAGGGCTCT CAGGACACCT TTTGTAAAGT CAGGTGATAA CTCCACTCTA 
AGGATTGAGA TAGATGATCC TGGGGTGCAC GCCATTGAGT TCCTCATCTT TGACGAGACA 
CAGAACAAAT GGTTTAAAAA CAATGGCCAG AATTTTCAGG TTCAGTTCCA GTCGAGCCGC 
CATCAGGGTA CTGGTGCATC TGGTGCCTCC TCTTCTGCTA CTTCTACCTT GGTGCCAGAG 
GATCTTGTGC AGATCCAAGC TTACCTTCGG TGGGAAAGAA GGGGAAAGCA GTCATACACA 
CCAGAGCAAG AAAAGGAGGA GTATGAAGCT GCACGAGCTG AGTTAATAGA GGAAGTAAAC 
AGAGGTGTTT CTTTAGAGAA GCTTCGAGCT AAATTGACAA AAGCACCTGA AGCACCTGAG 
TCGGATGAAA GTAAATCTTC TGCATCTCGA ATGCCCATCG GTAAACTTCC AGAGGATCTT 
GTACAGGTGC AGGCTTATAT AAGGTGGGAG CAAGCGGGCA AGCCAAACTA TCCTCCTGAG 
AAGCAACTGG TAGAATTTGA GGAAGCAAGG AAGGAACTGC AGGCTGAGGT GGACAAGGGA 
ATCTCTATTG ATCAGTTGAG GCAGAAAATT TTGAAAGGAA ACATTGAGAG TAAAGTTTCC 
AAGCAGCTGA AGAACAAGAA GTACTTCTCT GTAGAAAGGA TTCAGCGCAA AAAGAGAGAT 
ATCACACAAC TTCTCAGTAA ACATAAGCAT ACACTTGTGG AAGATAAAGT AGAGGTTGTA 
CCAAAACAAC CAACTGTTCT TGATCTCTTC ACCAAGTCTT TACATGAGAA GGATGGCTGT 
GAAGTTCTAA GCAGAAAGCT CTTCAAGTTC GGCGATAAAG AGATACTGGC AATTTCTACC 
AAGGTTCAAA ATAAAACAGA AGTTCACTTG GCAACAAACC ATACCGACCC ACTTATTCTT 
CACTGGTCTT TGGCAAAAAA TGCTGGAGAA TGGAAGGCAC CTTCTCCAAA TATATTGCCA 
TCTGGTTCCA CATTGCTGGA CAAGGCGTGT GAAACTGAAT TTACTAAATC TGAATTGGAT 
GGTTTGCATT ACCAGGTTGT TGAGATAGAG CTTGATGATG GAGGATACAA AGGAATGCCA 
TTTGTTCTTC GGTCTGGTGA AACATGGATA AAAAATAATG GTTCTGATTT TTTCCTAGAT 
TTCAGCACCC ATGATGTCAG AAATATTAAG TTAAAGGGCA ATGGTGATGC TGGTAAAGGT 
ACTGCTAAGG CATTGCTGGA GAGAATAGCA GATCTGGAGG AAGATGCCCA GCGATCTCTT 
ATGCACAGAT TCAATATTGC AGCAGATCTA GCTGACCAAG CCAGAGATGC TGGACTTTTG 
GGTATTGTTG GGCTTTTTGT TTGGATTAGA TTCATGGCTA CCAGGCAACT AACATGGAAT 
AAGAACTATA ATGTGAAGCC ACGTGAGATA AGCAAAGCAC AGGATAGGTT TACAGATGAT 
CTTGAGAATA TGTACAAAGC TTATCCACAG TACAGAGAGA TATTAAGAAT GATAATGGCT 
GCTGTTGGTC GCGGAGGTGA AGGTGATGTT GGTCAACGCA TTCGTGATGA GATATTAGTA 
ATACAGAGAA ATAATGACTG CAAAGGTGGA ATGATGGAAG AATGGCACCA GAAATTGCAC 
AACAATACAA GCCCAGATGA TGTAGTGATA TGCCAGGCCT TAATTGATTA TATCAAGAGT 
GACTTTGATA TAAGCGTTTA CTGGGACACC TTGAACAAAA ATGGCATAAC CAAAGAGCGT 
CTCTTGAGCT ATGATCGTGC TATTCATTCA GAACCAAATT TCAGAAGTGA ACAGAAGGCG 
GGTTTACTCC GTGACCTGGG AAATTACATG AGAAGCCTAA AGGCTGTGCA TTCTGGTGCT 
GATCTTGAAT CTGCTATAGC AAGTTGTATG GGATACAAAT CAGAGGGTGA AGGTTTCATG 
GTTGGTGTTC AGATCAATCC AGTGAAGGGT TTACCATCTG GATTTCCGGA GTTGCTTGAA 
TTTGTGCTTG AACATGTTGA GGATAAATCA GCGGAACCAC TTCTTGAGGG GCTATTGGAA 
GCTCGAGTTG AACTGCGCCC TTTGCTTCTT GATTCGCGTG AACGCATGAA AGATCTTATA 
TTTTTGGACA TTGCTCTTGA TTCTACCTTC AGGACAGCAA TTGAAAGGTC ATATGAGGAG 
CTGAATGATG CAGCCCCAGA GAAAATAATG TACTTCATCA GTCTTGTCCT TGAAAATCTT 
GCGCTTTCAA TTGACGACAA TGAAGACATC CTGTATTGTT TAAAGGGATG GAACCAAGCC 
TTGGAAATGG CTAAGCAAAA AGACGACCAA TGGGCGCTCT ATGCTAAAGC ATTTCTTGAC 
AGAAACAGAC TTGCCCTTGC GAGCAAGGGA GAACAATACC ATAATATGAT GCAGCCCTCT 
GCTGAGTATC TTGGCTCGTT ACTCAGCATA GACCAATGGG CAGTCAATAT CTTCACAGAA 
GAAATTATAC GCGGTGGATC AGCTGCTACT CTGTCTGCTC TTCTGAACCG ATTTGATCCT 
GTTTTAAGGA ATGTTGCTCA CCTCGGAAGT TGGCAGGTTA TAAGCCCGGT TGAAGTATCA 
GGTTATGTGG TTGTGGTTGA TGAGTTACTT GCTGTCCAGA ACAAATCTTA TGATAAACCA 
ACCATCCTTG TGGCAAAGAG TGTCAAGGGA GAGGAAGAAA TACCAGATGG AGTAGTTGGT 
GTAATTACAC CTGATATGCC AGATGTTCTG TCTCATGTGT CAGTCCGAGC AAGGAATAGC 
AAGGTACTGT TTGCGACCTG TTTTGACCAC ACCACTCTAT CTGAACTTGA AGGATATGAT 
CAGAAACTGT TTTCCTTCAA GCCTACTTCT GCAGATATAA CCTATAGGGA GATCACAGAG 
AGTGAACTTC AGCAATCAAG TTCTCCAAAT GCAGAAGTTG GCCATGCAGT ACCATCTATT 
TCATTGGCCA AGAAGAAATT TCTTGGAAAA TATGCAATAT CAGCCGAAGA ATTCTCTGAG 
GAAATGGTTG GGGCCAAGTC TCGGAATATA GCATACCTCA AAGGAAAAGT ACCTTCATGG 
GTCGGTGTCC CAACGTCAGT TGCGATACCA TTTGGCACTT TTGAGAAGGT TTTGTCAGAT 
GGGCTTAATA AGGAAGTAGC ACAGAGCATA GAGAAGCTTA AGATCAGACT TGCCCAAGAA 
GATTTTAGTG CTCTAGGTGA AATAAGAAAA GTCGTCCTTA ATCTTACTGC TCCTATGCAA 
TTGGTTAATG AGCTGAAGGA GAGGATGCTA GGCTCTGGAA TGCCCTGGCC TGGTGATGAA 
GGAGACAAGC GTTGGGAGCA AGCATGGATG GCTATTAAAA AGGTTTGGGC ATCAAAATGG 
AACGAAAGAG CATATTTTAG CACACGCAAG GTGAAACTTG ATCATGAGTA CCTTTCGATG 
GCTGTTCTCG TGCAAGAAGT TGTGAATGCA GATTATGCTT TTGTCATTCA TACCACAAAC 
CCATCGTCTG GAGATTCTTC TGAGATATAT GCTGAAGTGG TGAAAGGGCT TGGCGAGACC 
CTCGTGGGAG CCTATCCTGG TCGTGCTATG AGCTTTGTTT GCAAAAAAGA TGACCTTGAC 
TCTCCCAAGT TACTTGGTTA CCCAAGCAAG CCAATTGGTC TCTTCATAAG GCAATCAATC 
ATCTTCCGTT CCGACTCCAA CGGTGAGGAC CTGGAAGGTT ATGCTGGAGC AGGATTATAT 
GATAGTGTAC CGATGGATGA GGAGGATGAG GTTGTACTTG ATTATACAAC TGACCCTCTT 
ATAGTAGACC GTGGATTCCG AAGCTCAATC CTCTCAAGCA TAGCACGGGC TGGCCATGCC 
ATCGAGGAGC TATATGGTTC TCCTCAGGAC GTCGAGGGAG TAGTGAAGGA TGGAAAAATC 
TATGTAGTCC AGACAAGACC ACAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001348353.1
CDS	337..4746
Translation	MSGFSAAANAAAAERCALAFRARPAASSPAKRQQQPQPASLRRSGGQRRPTTLSASSRGPVVPRAVATSADRASPDLIGKFTLDSNSELQVAVNPAPQGLVSEISLEVTNTSGSLILHWGALRPDKRDWILPSRKPDGTTVYKNRALRTPFVKSGDNSTLRIEIDDPGVHAIEFLIFDETQNKWFKNNGQNFQVQFQSSRHQGTGASGASSSATSTLVPEDLVQIQAYLRWERRGKQSYTPEQEKEEYEAARAELIEEVNRGVSLEKLRAKLTKAPEAPESDESKSSASRMPIGKLPEDLVQVQAYIRWEQAGKPNYPPEKQLVEFEEARKELQAEVDKGISIDQLRQKILKGNIESKVSKQLKNKKYFSVERIQRKKRDITQLLSKHKHTLVEDKVEVVPKQPTVLDLFTKSLHEKDGCEVLSRKLFKFGDKEILAISTKVQNKTEVHLATNHTDPLILHWSLAKNAGEWKAPSPNILPSGSTLLDKACETEFTKSELDGLHYQVVEIELDDGGYKGMPFVLRSGETWIKNNGSDFFLDFSTHDVRNIKLKGNGDAGKGTAKALLERIADLEEDAQRSLMHRFNIAADLADQARDAGLLGIVGLFVWIRFMATRQLTWNKNYNVKPREISKAQDRFTDDLENMYKAYPQYREILRMIMAAVGRGGEGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNNTSPDDVVICQALIDYIKSDFDISVYWDTLNKNGITKERLLSYDRAIHSEPNFRSEQKAGLLRDLGNYMRSLKAVHSGADLESAIASCMGYKSEGEGFMVGVQINPVKGLPSGFPELLEFVLEHVEDKSAEPLLEGLLEARVELRPLLLDSRERMKDLIFLDIALDSTFRTAIERSYEELNDAAPEKIMYFISLVLENLALSIDDNEDILYCLKGWNQALEMAKQKDDQWALYAKAFLDRNRLALASKGEQYHNMMQPSAEYLGSLLSIDQWAVNIFTEEIIRGGSAATLSALLNRFDPVLRNVAHLGSWQVISPVEVSGYVVVVDELLAVQNKSYDKPTILVAKSVKGEEEIPDGVVGVITPDMPDVLSHVSVRARNSKVLFATCFDHTTLSELEGYDQKLFSFKPTSADITYREITESELQQSSSPNAEVGHAVPSISLAKKKFLGKYAISAEEFSEEMVGAKSRNIAYLKGKVPSWVGVPTSVAIPFGTFEKVLSDGLNKEVAQSIEKLKIRLAQEDFSALGEIRKVVLNLTAPMQLVNELKERMLGSGMPWPGDEGDKRWEQAWMAIKKVWASKWNERAYFSTRKVKLDHEYLSMAVLVQEVVNADYAFVIHTTNPSSGDSSEIYAEVVKGLGETLVGAYPGRAMSFVCKKDDLDSPKLLGYPSKPIGLFIRQSIIFRSDSNGEDLEGYAGAGLYDSVPMDEEDEVVLDYTTDPLIVDRGFRSSILSSIARAGHAIEELYGSPQDVEGVVKDGKIYVVQTRPQM

Target ORF information:

RefSeq Version	NM_001361424.1
Organism	Zea mays
Definition	Zea mays Alpha-glucan water dikinase 1 chloroplastic (LOC100381411), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001361424.1

ORF Insert Sequence:

ATGTCCGGAT TCAGTGCCGC GGCCAACGCA GCGGCGGCTG AGCGGTGCGC GCTCGCGTTC 
CGCGCACGGC CCGCGGCCTC CTCGCCAGCG AAGCGGCAGC AGCAGCCGCA GCCAGCGTCC 
CTCCGACGCA GCGGGGGCCA GCGCCGCCCC ACGACGCTCT CCGCCTCTAG CCGCGGCCCC 
GTCGTGCCGC GCGCCGTCGC CACGTCCGCG GACCGCGCGT CCCCCGACCT TATCGGAAAG 
TTCACGCTGG ATTCCAACTC CGAGCTCCAG GTCGCAGTGA ACCCAGCGCC GCAGGGTTTG 
GTGTCAGAGA TTAGCCTGGA GGTGACCAAC ACAAGCGGTT CCCTGATTTT GCATTGGGGA 
GCCCTTCGCC CGGACAAGAG AGATTGGATC CTCCCGTCCA GAAAACCTGA TGGAACGACA 
GTGTACAAGA ACAGGGCTCT CAGGACACCT TTTGTAAAGT CAGGTGATAA CTCCACTCTA 
AGGATTGAGA TAGATGATCC TGGGGTGCAC GCCATTGAGT TCCTCATCTT TGACGAGACA 
CAGAACAAAT GGTTTAAAAA CAATGGCCAG AATTTTCAGG TTCAGTTCCA GTCGAGCCGC 
CATCAGGGTA CTGGTGCATC TGGTGCCTCC TCTTCTGCTA CTTCTACCTT GGTGCCAGAG 
GATCTTGTGC AGATCCAAGC TTACCTTCGG TGGGAAAGAA GGGGAAAGCA GTCATACACA 
CCAGAGCAAG AAAAGGAGGA GTATGAAGCT GCACGAGCTG AGTTAATAGA GGAAGTAAAC 
AGAGGTGTTT CTTTAGAGAA GCTTCGAGCT AAATTGACAA AAGCACCTGA AGCACCTGAG 
TCGGATGAAA GTAAATCTTC TGCATCTCGA ATGCCCATCG GTAAACTTCC AGAGGATCTT 
GTACAGGTGC AGGCTTATAT AAGGTGGGAG CAAGCGGGCA AGCCAAACTA TCCTCCTGAG 
AAGCAACTGG TAGAATTTGA GGAAGCAAGG AAGGAACTGC AGGCTGAGGT GGACAAGGGA 
ATCTCTATTG ATCAGTTGAG GCAGAAAATT TTGAAAGGAA ACATTGAGAG TAAAGTTTCC 
AAGCAGCTGA AGAACAAGAA GTACTTCTCT GTAGAAAGGA TTCAGCGCAA AAAGAGAGAT 
ATCACACAAC TTCTCAGTAA ACATAAGCAT ACACTTGTGG AAGATAAAGT AGAGGTTGTA 
CCAAAACAAC CAACTGTTCT TGATCTCTTC ACCAAGTCTT TACATGAGAA GGATGGCTGT 
GAAGTTCTAA GCAGAAAGCT CTTCAAGTTC GGCGATAAAG AGATACTGGC AATTTCTACC 
AAGGTTCAAA ATAAAACAGA AGTTCACTTG GCAACAAACC ATACCGACCC ACTTATTCTT 
CACTGGTCTT TGGCAAAAAA TGCTGGAGAA TGGAAGGCAC CTTCTCCAAA TATATTGCCA 
TCTGGTTCCA CATTGCTGGA CAAGGCGTGT GAAACTGAAT TTACTAAATC TGAATTGGAT 
GGTTTGCATT ACCAGGTTGT TGAGATAGAG CTTGATGATG GAGGATACAA AGGAATGCCA 
TTTGTTCTTC GGTCTGGTGA AACATGGATA AAAAATAATG GTTCTGATTT TTTCCTAGAT 
TTCAGCACCC ATGATGTCAG AAATATTAAG TTAAAGGGCA ATGGTGATGC TGGTAAAGGT 
ACTGCTAAGG CATTGCTGGA GAGAATAGCA GATCTGGAGG AAGATGCCCA GCGATCTCTT 
ATGCACAGAT TCAATATTGC AGCAGATCTA GCTGACCAAG CCAGAGATGC TGGACTTTTG 
GGTATTGTTG GGCTTTTTGT TTGGATTAGA TTCATGGCTA CCAGGCAACT AACATGGAAT 
AAGAACTATA ATGTGAAGCC ACGTGAGATA AGCAAAGCAC AGGATAGGTT TACAGATGAT 
CTTGAGAATA TGTACAAAGC TTATCCACAG TACAGAGAGA TATTAAGAAT GATAATGGCT 
GCTGTTGGTC GCGGAGGTGA AGGTGATGTT GGTCAACGCA TTCGTGATGA GATATTAGTA 
ATACAGAGAA ATAATGACTG CAAAGGTGGA ATGATGGAAG AATGGCACCA GAAATTGCAC 
AACAATACAA GCCCAGATGA TGTAGTGATA TGCCAGGCCT TAATTGATTA TATCAAGAGT 
GACTTTGATA TAAGCGTTTA CTGGGACACC TTGAACAAAA ATGGCATAAC CAAAGAGCGT 
CTCTTGAGCT ATGATCGTGC TATTCATTCA GAACCAAATT TCAGAAGTGA ACAGAAGGCG 
GGTTTACTCC GTGACCTGGG AAATTACATG AGAAGCCTAA AGGCTGTGCA TTCTGGTGCT 
GATCTTGAAT CTGCTATAGC AAGTTGTATG GGATACAAAT CAGAGGGTGA AGGTTTCATG 
GTTGGTGTTC AGATCAATCC AGTGAAGGGT TTACCATCTG GATTTCCGGA GTTGCTTGAA 
TTTGTGCTTG AACATGTTGA GGATAAATCA GCGGAACCAC TTCTTGAGGG GCTATTGGAA 
GCTCGAGTTG AACTGCGCCC TTTGCTTCTT GATTCGCGTG AACGCATGAA AGATCTTATA 
TTTTTGGACA TTGCTCTTGA TTCTACCTTC AGGACAGCAA TTGAAAGGTC ATATGAGGAG 
CTGAATGATG CAGCCCCAGA GAAAATAATG TACTTCATCA GTCTTGTCCT TGAAAATCTT 
GCGCTTTCAA TTGACGACAA TGAAGACATC CTGTATTGTT TAAAGGGATG GAACCAAGCC 
TTGGAAATGG CTAAGCAAAA AGACGACCAA TGGGCGCTCT ATGCTAAAGC ATTTCTTGAC 
AGAAACAGAC TTGCCCTTGC GAGCAAGGGA GAACAATACC ATAATATGAT GCAGCCCTCT 
GCTGAGTATC TTGGCTCGTT ACTCAGCATA GACCAATGGG CAGTCAATAT CTTCACAGAA 
GAAATTATAC GCGGTGGATC AGCTGCTACT CTGTCTGCTC TTCTGAACCG ATTTGATCCT 
GTTTTAAGGA ATGTTGCTCA CCTCGGAAGT TGGCAGGTTA TAAGCCCGGT TGAAGTATCA 
GGTTATGTGG TTGTGGTTGA TGAGTTACTT GCTGTCCAGA ACAAATCTTA TGATAAACCA 
ACCATCCTTG TGGCAAAGAG TGTCAAGGGA GAGGAAGAAA TACCAGATGG AGTAGTTGGT 
GTAATTACAC CTGATATGCC AGATGTTCTG TCTCATGTGT CAGTCCGAGC AAGGAATAGC 
AAGGTACTGT TTGCGACCTG TTTTGACCAC ACCACTCTAT CTGAACTTGA AGGATATGAT 
CAGAAACTGT TTTCCTTCAA GCCTACTTCT GCAGATATAA CCTATAGGGA GATCACAGAG 
AGTGAACTTC AGCAATCAAG TTCTCCAAAT GCAGAAGTTG GCCATGCAGT ACCATCTATT 
TCATTGGCCA AGAAGAAATT TCTTGGAAAA TATGCAATAT CAGCCGAAGA ATTCTCTGAG 
GAAATGGTTG GGGCCAAGTC TCGGAATATA GCATACCTCA AAGGAAAAGT ACCTTCATGG 
GTCGGTGTCC CAACGTCAGT TGCGATACCA TTTGGCACTT TTGAGAAGGT TTTGTCAGAT 
GGGCTTAATA AGGAAGTAGC ACAGAGCATA GAGAAGCTTA AGATCAGACT TGCCCAAGAA 
GATTTTAGTG CTCTAGGTGA AATAAGAAAA GTCGTCCTTA ATCTTACTGC TCCTATGCAA 
TTGGTTAATG AGCTGAAGGA GAGGATGCTA GGCTCTGGAA TGCCCTGGCC TGGTGATGAA 
GGAGACAAGC GTTGGGAGCA AGCATGGATG GCTATTAAAA AGGTTTGGGC ATCAAAATGG 
AACGAAAGAG CATATTTTAG CACACGCAAG GTGAAACTTG ATCATGAGTA CCTTTCGATG 
GCTGTTCTCG TGCAAGAAGT TGTGAATGCA GATTATGCTT TTGTCATTCA TACCACAAAC 
CCATCGTCTG GAGATTCTTC TGAGATATAT GCTGAAGTGG TGAAAGGGCT TGGCGAGACC 
CTCGTGGGAG CCTATCCTGG TCGTGCTATG AGCTTTGTTT GCAAAAAAGA TGACCTTGAC 
TCTCCCAAGT TACTTGGTTA CCCAAGCAAG CCAATTGGTC TCTTCATAAG GCAATCAATC 
ATCTTCCGTT CCGACTCCAA CGGTGAGGAC CTGGAAGGTT ATGCTGGAGC AGGATTATAT 
GATAGTGTAC CGATGGATGA GGAGGATGAG GTTGTACTTG ATTATACAAC TGACCCTCTT 
ATAGTAGACC GTGGATTCCG AAGCTCAATC CTCTCAAGCA TAGCACGGGC TGGCCATGCC 
ATCGAGGAGC TATATGGTTC TCCTCAGGAC GTCGAGGGAG TAGTGAAGGA TGGAAAAATC 
TATGTAGTCC AGACAAGACC ACAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AY109804 ( XM_008649791.2 ) cDNA ORF clone, Zea mays -> XP_008648013.1 Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OZa29821
Clone ID Related Accession (Same CDS sequence)	XM_008649791.2 , XM_020539039.1 , XM_020539038.1 , NM_001361424.1 , XM_020539039.2
Accession Version	XM_008649791.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	alpha-glucan water dikinase 1 chloroplastic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024464.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2017 this sequence version replaced XM_008649791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAT TCAGTGCCGC GGCCAACGCA GCGGCGGCTG AGCGGTGCGC GCTCGCGTTC 
CGCGCACGGC CCGCGGCCTC CTCGCCAGCG AAGCGGCAGC AGCAGCCGCA GCCAGCGTCC 
CTCCGACGCA GCGGGGGCCA GCGCCGCCCC ACGACGCTCT CCGCCTCTAG CCGCGGCCCC 
GTCGTGCCGC GCGCCGTCGC CACGTCCGCG GACCGCGCGT CCCCCGACCT TATCGGAAAG 
TTCACGCTGG ATTCCAACTC CGAGCTCCAG GTCGCAGTGA ACCCAGCGCC GCAGGGTTTG 
GTGTCAGAGA TTAGCCTGGA GGTGACCAAC ACAAGCGGTT CCCTGATTTT GCATTGGGGA 
GCCCTTCGCC CGGACAAGAG AGATTGGATC CTCCCGTCCA GAAAACCTGA TGGAACGACA 
GTGTACAAGA ACAGGGCTCT CAGGACACCT TTTGTAAAGT CAGGTGATAA CTCCACTCTA 
AGGATTGAGA TAGATGATCC TGGGGTGCAC GCCATTGAGT TCCTCATCTT TGACGAGACA 
CAGAACAAAT GGTTTAAAAA CAATGGCCAG AATTTTCAGG TTCAGTTCCA GTCGAGCCGC 
CATCAGGGTA CTGGTGCATC TGGTGCCTCC TCTTCTGCTA CTTCTACCTT GGTGCCAGAG 
GATCTTGTGC AGATCCAAGC TTACCTTCGG TGGGAAAGAA GGGGAAAGCA GTCATACACA 
CCAGAGCAAG AAAAGGAGGA GTATGAAGCT GCACGAGCTG AGTTAATAGA GGAAGTAAAC 
AGAGGTGTTT CTTTAGAGAA GCTTCGAGCT AAATTGACAA AAGCACCTGA AGCACCTGAG 
TCGGATGAAA GTAAATCTTC TGCATCTCGA ATGCCCATCG GTAAACTTCC AGAGGATCTT 
GTACAGGTGC AGGCTTATAT AAGGTGGGAG CAAGCGGGCA AGCCAAACTA TCCTCCTGAG 
AAGCAACTGG TAGAATTTGA GGAAGCAAGG AAGGAACTGC AGGCTGAGGT GGACAAGGGA 
ATCTCTATTG ATCAGTTGAG GCAGAAAATT TTGAAAGGAA ACATTGAGAG TAAAGTTTCC 
AAGCAGCTGA AGAACAAGAA GTACTTCTCT GTAGAAAGGA TTCAGCGCAA AAAGAGAGAT 
ATCACACAAC TTCTCAGTAA ACATAAGCAT ACACTTGTGG AAGATAAAGT AGAGGTTGTA 
CCAAAACAAC CAACTGTTCT TGATCTCTTC ACCAAGTCTT TACATGAGAA GGATGGCTGT 
GAAGTTCTAA GCAGAAAGCT CTTCAAGTTC GGCGATAAAG AGATACTGGC AATTTCTACC 
AAGGTTCAAA ATAAAACAGA AGTTCACTTG GCAACAAACC ATACCGACCC ACTTATTCTT 
CACTGGTCTT TGGCAAAAAA TGCTGGAGAA TGGAAGGCAC CTTCTCCAAA TATATTGCCA 
TCTGGTTCCA CATTGCTGGA CAAGGCGTGT GAAACTGAAT TTACTAAATC TGAATTGGAT 
GGTTTGCATT ACCAGGTTGT TGAGATAGAG CTTGATGATG GAGGATACAA AGGAATGCCA 
TTTGTTCTTC GGTCTGGTGA AACATGGATA AAAAATAATG GTTCTGATTT TTTCCTAGAT 
TTCAGCACCC ATGATGTCAG AAATATTAAG TTAAAGGGCA ATGGTGATGC TGGTAAAGGT 
ACTGCTAAGG CATTGCTGGA GAGAATAGCA GATCTGGAGG AAGATGCCCA GCGATCTCTT 
ATGCACAGAT TCAATATTGC AGCAGATCTA GCTGACCAAG CCAGAGATGC TGGACTTTTG 
GGTATTGTTG GGCTTTTTGT TTGGATTAGA TTCATGGCTA CCAGGCAACT AACATGGAAT 
AAGAACTATA ATGTGAAGCC ACGTGAGATA AGCAAAGCAC AGGATAGGTT TACAGATGAT 
CTTGAGAATA TGTACAAAGC TTATCCACAG TACAGAGAGA TATTAAGAAT GATAATGGCT 
GCTGTTGGTC GCGGAGGTGA AGGTGATGTT GGTCAACGCA TTCGTGATGA GATATTAGTA 
ATACAGAGAA ATAATGACTG CAAAGGTGGA ATGATGGAAG AATGGCACCA GAAATTGCAC 
AACAATACAA GCCCAGATGA TGTAGTGATA TGCCAGGCCT TAATTGATTA TATCAAGAGT 
GACTTTGATA TAAGCGTTTA CTGGGACACC TTGAACAAAA ATGGCATAAC CAAAGAGCGT 
CTCTTGAGCT ATGATCGTGC TATTCATTCA GAACCAAATT TCAGAAGTGA ACAGAAGGCG 
GGTTTACTCC GTGACCTGGG AAATTACATG AGAAGCCTAA AGGCTGTGCA TTCTGGTGCT 
GATCTTGAAT CTGCTATAGC AAGTTGTATG GGATACAAAT CAGAGGGTGA AGGTTTCATG 
GTTGGTGTTC AGATCAATCC AGTGAAGGGT TTACCATCTG GATTTCCGGA GTTGCTTGAA 
TTTGTGCTTG AACATGTTGA GGATAAATCA GCGGAACCAC TTCTTGAGGG GCTATTGGAA 
GCTCGAGTTG AACTGCGCCC TTTGCTTCTT GATTCGCGTG AACGCATGAA AGATCTTATA 
TTTTTGGACA TTGCTCTTGA TTCTACCTTC AGGACAGCAA TTGAAAGGTC ATATGAGGAG 
CTGAATGATG CAGCCCCAGA GAAAATAATG TACTTCATCA GTCTTGTCCT TGAAAATCTT 
GCGCTTTCAA TTGACGACAA TGAAGACATC CTGTATTGTT TAAAGGGATG GAACCAAGCC 
TTGGAAATGG CTAAGCAAAA AGACGACCAA TGGGCGCTCT ATGCTAAAGC ATTTCTTGAC 
AGAAACAGAC TTGCCCTTGC GAGCAAGGGA GAACAATACC ATAATATGAT GCAGCCCTCT 
GCTGAGTATC TTGGCTCGTT ACTCAGCATA GACCAATGGG CAGTCAATAT CTTCACAGAA 
GAAATTATAC GCGGTGGATC AGCTGCTACT CTGTCTGCTC TTCTGAACCG ATTTGATCCT 
GTTTTAAGGA ATGTTGCTCA CCTCGGAAGT TGGCAGGTTA TAAGCCCGGT TGAAGTATCA 
GGTTATGTGG TTGTGGTTGA TGAGTTACTT GCTGTCCAGA ACAAATCTTA TGATAAACCA 
ACCATCCTTG TGGCAAAGAG TGTCAAGGGA GAGGAAGAAA TACCAGATGG AGTAGTTGGT 
GTAATTACAC CTGATATGCC AGATGTTCTG TCTCATGTGT CAGTCCGAGC AAGGAATAGC 
AAGGTACTGT TTGCGACCTG TTTTGACCAC ACCACTCTAT CTGAACTTGA AGGATATGAT 
CAGAAACTGT TTTCCTTCAA GCCTACTTCT GCAGATATAA CCTATAGGGA GATCACAGAG 
AGTGAACTTC AGCAATCAAG TTCTCCAAAT GCAGAAGTTG GCCATGCAGT ACCATCTATT 
TCATTGGCCA AGAAGAAATT TCTTGGAAAA TATGCAATAT CAGCCGAAGA ATTCTCTGAG 
GAAATGGTTG GGGCCAAGTC TCGGAATATA GCATACCTCA AAGGAAAAGT ACCTTCATGG 
GTCGGTGTCC CAACGTCAGT TGCGATACCA TTTGGCACTT TTGAGAAGGT TTTGTCAGAT 
GGGCTTAATA AGGAAGTAGC ACAGAGCATA GAGAAGCTTA AGATCAGACT TGCCCAAGAA 
GATTTTAGTG CTCTAGGTGA AATAAGAAAA GTCGTCCTTA ATCTTACTGC TCCTATGCAA 
TTGGTTAATG AGCTGAAGGA GAGGATGCTA GGCTCTGGAA TGCCCTGGCC TGGTGATGAA 
GGAGACAAGC GTTGGGAGCA AGCATGGATG GCTATTAAAA AGGTTTGGGC ATCAAAATGG 
AACGAAAGAG CATATTTTAG CACACGCAAG GTGAAACTTG ATCATGAGTA CCTTTCGATG 
GCTGTTCTCG TGCAAGAAGT TGTGAATGCA GATTATGCTT TTGTCATTCA TACCACAAAC 
CCATCGTCTG GAGATTCTTC TGAGATATAT GCTGAAGTGG TGAAAGGGCT TGGCGAGACC 
CTCGTGGGAG CCTATCCTGG TCGTGCTATG AGCTTTGTTT GCAAAAAAGA TGACCTTGAC 
TCTCCCAAGT TACTTGGTTA CCCAAGCAAG CCAATTGGTC TCTTCATAAG GCAATCAATC 
ATCTTCCGTT CCGACTCCAA CGGTGAGGAC CTGGAAGGTT ATGCTGGAGC AGGATTATAT 
GATAGTGTAC CGATGGATGA GGAGGATGAG GTTGTACTTG ATTATACAAC TGACCCTCTT 
ATAGTAGACC GTGGATTCCG AAGCTCAATC CTCTCAAGCA TAGCACGGGC TGGCCATGCC 
ATCGAGGAGC TATATGGTTC TCCTCAGGAC GTCGAGGGAG TAGTGAAGGA TGGAAAAATC 
TATGTAGTCC AGACAAGACC ACAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008648013.1
CDS	371..4780
Translation	MSGFSAAANAAAAERCALAFRARPAASSPAKRQQQPQPASLRRSGGQRRPTTLSASSRGPVVPRAVATSADRASPDLIGKFTLDSNSELQVAVNPAPQGLVSEISLEVTNTSGSLILHWGALRPDKRDWILPSRKPDGTTVYKNRALRTPFVKSGDNSTLRIEIDDPGVHAIEFLIFDETQNKWFKNNGQNFQVQFQSSRHQGTGASGASSSATSTLVPEDLVQIQAYLRWERRGKQSYTPEQEKEEYEAARAELIEEVNRGVSLEKLRAKLTKAPEAPESDESKSSASRMPIGKLPEDLVQVQAYIRWEQAGKPNYPPEKQLVEFEEARKELQAEVDKGISIDQLRQKILKGNIESKVSKQLKNKKYFSVERIQRKKRDITQLLSKHKHTLVEDKVEVVPKQPTVLDLFTKSLHEKDGCEVLSRKLFKFGDKEILAISTKVQNKTEVHLATNHTDPLILHWSLAKNAGEWKAPSPNILPSGSTLLDKACETEFTKSELDGLHYQVVEIELDDGGYKGMPFVLRSGETWIKNNGSDFFLDFSTHDVRNIKLKGNGDAGKGTAKALLERIADLEEDAQRSLMHRFNIAADLADQARDAGLLGIVGLFVWIRFMATRQLTWNKNYNVKPREISKAQDRFTDDLENMYKAYPQYREILRMIMAAVGRGGEGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNNTSPDDVVICQALIDYIKSDFDISVYWDTLNKNGITKERLLSYDRAIHSEPNFRSEQKAGLLRDLGNYMRSLKAVHSGADLESAIASCMGYKSEGEGFMVGVQINPVKGLPSGFPELLEFVLEHVEDKSAEPLLEGLLEARVELRPLLLDSRERMKDLIFLDIALDSTFRTAIERSYEELNDAAPEKIMYFISLVLENLALSIDDNEDILYCLKGWNQALEMAKQKDDQWALYAKAFLDRNRLALASKGEQYHNMMQPSAEYLGSLLSIDQWAVNIFTEEIIRGGSAATLSALLNRFDPVLRNVAHLGSWQVISPVEVSGYVVVVDELLAVQNKSYDKPTILVAKSVKGEEEIPDGVVGVITPDMPDVLSHVSVRARNSKVLFATCFDHTTLSELEGYDQKLFSFKPTSADITYREITESELQQSSSPNAEVGHAVPSISLAKKKFLGKYAISAEEFSEEMVGAKSRNIAYLKGKVPSWVGVPTSVAIPFGTFEKVLSDGLNKEVAQSIEKLKIRLAQEDFSALGEIRKVVLNLTAPMQLVNELKERMLGSGMPWPGDEGDKRWEQAWMAIKKVWASKWNERAYFSTRKVKLDHEYLSMAVLVQEVVNADYAFVIHTTNPSSGDSSEIYAEVVKGLGETLVGAYPGRAMSFVCKKDDLDSPKLLGYPSKPIGLFIRQSIIFRSDSNGEDLEGYAGAGLYDSVPMDEEDEVVLDYTTDPLIVDRGFRSSILSSIARAGHAIEELYGSPQDVEGVVKDGKIYVVQTRPQM

Target ORF information:

RefSeq Version	XM_008649791.2
Organism	Zea mays
Definition	Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008649791.2

ORF Insert Sequence:

ATGTCCGGAT TCAGTGCCGC GGCCAACGCA GCGGCGGCTG AGCGGTGCGC GCTCGCGTTC 
CGCGCACGGC CCGCGGCCTC CTCGCCAGCG AAGCGGCAGC AGCAGCCGCA GCCAGCGTCC 
CTCCGACGCA GCGGGGGCCA GCGCCGCCCC ACGACGCTCT CCGCCTCTAG CCGCGGCCCC 
GTCGTGCCGC GCGCCGTCGC CACGTCCGCG GACCGCGCGT CCCCCGACCT TATCGGAAAG 
TTCACGCTGG ATTCCAACTC CGAGCTCCAG GTCGCAGTGA ACCCAGCGCC GCAGGGTTTG 
GTGTCAGAGA TTAGCCTGGA GGTGACCAAC ACAAGCGGTT CCCTGATTTT GCATTGGGGA 
GCCCTTCGCC CGGACAAGAG AGATTGGATC CTCCCGTCCA GAAAACCTGA TGGAACGACA 
GTGTACAAGA ACAGGGCTCT CAGGACACCT TTTGTAAAGT CAGGTGATAA CTCCACTCTA 
AGGATTGAGA TAGATGATCC TGGGGTGCAC GCCATTGAGT TCCTCATCTT TGACGAGACA 
CAGAACAAAT GGTTTAAAAA CAATGGCCAG AATTTTCAGG TTCAGTTCCA GTCGAGCCGC 
CATCAGGGTA CTGGTGCATC TGGTGCCTCC TCTTCTGCTA CTTCTACCTT GGTGCCAGAG 
GATCTTGTGC AGATCCAAGC TTACCTTCGG TGGGAAAGAA GGGGAAAGCA GTCATACACA 
CCAGAGCAAG AAAAGGAGGA GTATGAAGCT GCACGAGCTG AGTTAATAGA GGAAGTAAAC 
AGAGGTGTTT CTTTAGAGAA GCTTCGAGCT AAATTGACAA AAGCACCTGA AGCACCTGAG 
TCGGATGAAA GTAAATCTTC TGCATCTCGA ATGCCCATCG GTAAACTTCC AGAGGATCTT 
GTACAGGTGC AGGCTTATAT AAGGTGGGAG CAAGCGGGCA AGCCAAACTA TCCTCCTGAG 
AAGCAACTGG TAGAATTTGA GGAAGCAAGG AAGGAACTGC AGGCTGAGGT GGACAAGGGA 
ATCTCTATTG ATCAGTTGAG GCAGAAAATT TTGAAAGGAA ACATTGAGAG TAAAGTTTCC 
AAGCAGCTGA AGAACAAGAA GTACTTCTCT GTAGAAAGGA TTCAGCGCAA AAAGAGAGAT 
ATCACACAAC TTCTCAGTAA ACATAAGCAT ACACTTGTGG AAGATAAAGT AGAGGTTGTA 
CCAAAACAAC CAACTGTTCT TGATCTCTTC ACCAAGTCTT TACATGAGAA GGATGGCTGT 
GAAGTTCTAA GCAGAAAGCT CTTCAAGTTC GGCGATAAAG AGATACTGGC AATTTCTACC 
AAGGTTCAAA ATAAAACAGA AGTTCACTTG GCAACAAACC ATACCGACCC ACTTATTCTT 
CACTGGTCTT TGGCAAAAAA TGCTGGAGAA TGGAAGGCAC CTTCTCCAAA TATATTGCCA 
TCTGGTTCCA CATTGCTGGA CAAGGCGTGT GAAACTGAAT TTACTAAATC TGAATTGGAT 
GGTTTGCATT ACCAGGTTGT TGAGATAGAG CTTGATGATG GAGGATACAA AGGAATGCCA 
TTTGTTCTTC GGTCTGGTGA AACATGGATA AAAAATAATG GTTCTGATTT TTTCCTAGAT 
TTCAGCACCC ATGATGTCAG AAATATTAAG TTAAAGGGCA ATGGTGATGC TGGTAAAGGT 
ACTGCTAAGG CATTGCTGGA GAGAATAGCA GATCTGGAGG AAGATGCCCA GCGATCTCTT 
ATGCACAGAT TCAATATTGC AGCAGATCTA GCTGACCAAG CCAGAGATGC TGGACTTTTG 
GGTATTGTTG GGCTTTTTGT TTGGATTAGA TTCATGGCTA CCAGGCAACT AACATGGAAT 
AAGAACTATA ATGTGAAGCC ACGTGAGATA AGCAAAGCAC AGGATAGGTT TACAGATGAT 
CTTGAGAATA TGTACAAAGC TTATCCACAG TACAGAGAGA TATTAAGAAT GATAATGGCT 
GCTGTTGGTC GCGGAGGTGA AGGTGATGTT GGTCAACGCA TTCGTGATGA GATATTAGTA 
ATACAGAGAA ATAATGACTG CAAAGGTGGA ATGATGGAAG AATGGCACCA GAAATTGCAC 
AACAATACAA GCCCAGATGA TGTAGTGATA TGCCAGGCCT TAATTGATTA TATCAAGAGT 
GACTTTGATA TAAGCGTTTA CTGGGACACC TTGAACAAAA ATGGCATAAC CAAAGAGCGT 
CTCTTGAGCT ATGATCGTGC TATTCATTCA GAACCAAATT TCAGAAGTGA ACAGAAGGCG 
GGTTTACTCC GTGACCTGGG AAATTACATG AGAAGCCTAA AGGCTGTGCA TTCTGGTGCT 
GATCTTGAAT CTGCTATAGC AAGTTGTATG GGATACAAAT CAGAGGGTGA AGGTTTCATG 
GTTGGTGTTC AGATCAATCC AGTGAAGGGT TTACCATCTG GATTTCCGGA GTTGCTTGAA 
TTTGTGCTTG AACATGTTGA GGATAAATCA GCGGAACCAC TTCTTGAGGG GCTATTGGAA 
GCTCGAGTTG AACTGCGCCC TTTGCTTCTT GATTCGCGTG AACGCATGAA AGATCTTATA 
TTTTTGGACA TTGCTCTTGA TTCTACCTTC AGGACAGCAA TTGAAAGGTC ATATGAGGAG 
CTGAATGATG CAGCCCCAGA GAAAATAATG TACTTCATCA GTCTTGTCCT TGAAAATCTT 
GCGCTTTCAA TTGACGACAA TGAAGACATC CTGTATTGTT TAAAGGGATG GAACCAAGCC 
TTGGAAATGG CTAAGCAAAA AGACGACCAA TGGGCGCTCT ATGCTAAAGC ATTTCTTGAC 
AGAAACAGAC TTGCCCTTGC GAGCAAGGGA GAACAATACC ATAATATGAT GCAGCCCTCT 
GCTGAGTATC TTGGCTCGTT ACTCAGCATA GACCAATGGG CAGTCAATAT CTTCACAGAA 
GAAATTATAC GCGGTGGATC AGCTGCTACT CTGTCTGCTC TTCTGAACCG ATTTGATCCT 
GTTTTAAGGA ATGTTGCTCA CCTCGGAAGT TGGCAGGTTA TAAGCCCGGT TGAAGTATCA 
GGTTATGTGG TTGTGGTTGA TGAGTTACTT GCTGTCCAGA ACAAATCTTA TGATAAACCA 
ACCATCCTTG TGGCAAAGAG TGTCAAGGGA GAGGAAGAAA TACCAGATGG AGTAGTTGGT 
GTAATTACAC CTGATATGCC AGATGTTCTG TCTCATGTGT CAGTCCGAGC AAGGAATAGC 
AAGGTACTGT TTGCGACCTG TTTTGACCAC ACCACTCTAT CTGAACTTGA AGGATATGAT 
CAGAAACTGT TTTCCTTCAA GCCTACTTCT GCAGATATAA CCTATAGGGA GATCACAGAG 
AGTGAACTTC AGCAATCAAG TTCTCCAAAT GCAGAAGTTG GCCATGCAGT ACCATCTATT 
TCATTGGCCA AGAAGAAATT TCTTGGAAAA TATGCAATAT CAGCCGAAGA ATTCTCTGAG 
GAAATGGTTG GGGCCAAGTC TCGGAATATA GCATACCTCA AAGGAAAAGT ACCTTCATGG 
GTCGGTGTCC CAACGTCAGT TGCGATACCA TTTGGCACTT TTGAGAAGGT TTTGTCAGAT 
GGGCTTAATA AGGAAGTAGC ACAGAGCATA GAGAAGCTTA AGATCAGACT TGCCCAAGAA 
GATTTTAGTG CTCTAGGTGA AATAAGAAAA GTCGTCCTTA ATCTTACTGC TCCTATGCAA 
TTGGTTAATG AGCTGAAGGA GAGGATGCTA GGCTCTGGAA TGCCCTGGCC TGGTGATGAA 
GGAGACAAGC GTTGGGAGCA AGCATGGATG GCTATTAAAA AGGTTTGGGC ATCAAAATGG 
AACGAAAGAG CATATTTTAG CACACGCAAG GTGAAACTTG ATCATGAGTA CCTTTCGATG 
GCTGTTCTCG TGCAAGAAGT TGTGAATGCA GATTATGCTT TTGTCATTCA TACCACAAAC 
CCATCGTCTG GAGATTCTTC TGAGATATAT GCTGAAGTGG TGAAAGGGCT TGGCGAGACC 
CTCGTGGGAG CCTATCCTGG TCGTGCTATG AGCTTTGTTT GCAAAAAAGA TGACCTTGAC 
TCTCCCAAGT TACTTGGTTA CCCAAGCAAG CCAATTGGTC TCTTCATAAG GCAATCAATC 
ATCTTCCGTT CCGACTCCAA CGGTGAGGAC CTGGAAGGTT ATGCTGGAGC AGGATTATAT 
GATAGTGTAC CGATGGATGA GGAGGATGAG GTTGTACTTG ATTATACAAC TGACCCTCTT 
ATAGTAGACC GTGGATTCCG AAGCTCAATC CTCTCAAGCA TAGCACGGGC TGGCCATGCC 
ATCGAGGAGC TATATGGTTC TCCTCAGGAC GTCGAGGGAG TAGTGAAGGA TGGAAAAATC 
TATGTAGTCC AGACAAGACC ACAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AY109804 ( XM_020539039.1 ) cDNA ORF clone, Zea mays -> XP_020394628.1 Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OZa29821
Clone ID Related Accession (Same CDS sequence)	XM_008649791.2 , XM_020539039.1 , XM_020539038.1 , NM_001361424.1 , XM_020539039.2
Accession Version	XM_020539039.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1489852800000
Organism	Zea mays
Product	alpha-glucan water dikinase 1 chloroplastic isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024464.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAT TCAGTGCCGC GGCCAACGCA GCGGCGGCTG AGCGGTGCGC GCTCGCGTTC 
CGCGCACGGC CCGCGGCCTC CTCGCCAGCG AAGCGGCAGC AGCAGCCGCA GCCAGCGTCC 
CTCCGACGCA GCGGGGGCCA GCGCCGCCCC ACGACGCTCT CCGCCTCTAG CCGCGGCCCC 
GTCGTGCCGC GCGCCGTCGC CACGTCCGCG GACCGCGCGT CCCCCGACCT TATCGGAAAG 
TTCACGCTGG ATTCCAACTC CGAGCTCCAG GTCGCAGTGA ACCCAGCGCC GCAGGGTTTG 
GTGTCAGAGA TTAGCCTGGA GGTGACCAAC ACAAGCGGTT CCCTGATTTT GCATTGGGGA 
GCCCTTCGCC CGGACAAGAG AGATTGGATC CTCCCGTCCA GAAAACCTGA TGGAACGACA 
GTGTACAAGA ACAGGGCTCT CAGGACACCT TTTGTAAAGT CAGGTGATAA CTCCACTCTA 
AGGATTGAGA TAGATGATCC TGGGGTGCAC GCCATTGAGT TCCTCATCTT TGACGAGACA 
CAGAACAAAT GGTTTAAAAA CAATGGCCAG AATTTTCAGG TTCAGTTCCA GTCGAGCCGC 
CATCAGGGTA CTGGTGCATC TGGTGCCTCC TCTTCTGCTA CTTCTACCTT GGTGCCAGAG 
GATCTTGTGC AGATCCAAGC TTACCTTCGG TGGGAAAGAA GGGGAAAGCA GTCATACACA 
CCAGAGCAAG AAAAGGAGGA GTATGAAGCT GCACGAGCTG AGTTAATAGA GGAAGTAAAC 
AGAGGTGTTT CTTTAGAGAA GCTTCGAGCT AAATTGACAA AAGCACCTGA AGCACCTGAG 
TCGGATGAAA GTAAATCTTC TGCATCTCGA ATGCCCATCG GTAAACTTCC AGAGGATCTT 
GTACAGGTGC AGGCTTATAT AAGGTGGGAG CAAGCGGGCA AGCCAAACTA TCCTCCTGAG 
AAGCAACTGG TAGAATTTGA GGAAGCAAGG AAGGAACTGC AGGCTGAGGT GGACAAGGGA 
ATCTCTATTG ATCAGTTGAG GCAGAAAATT TTGAAAGGAA ACATTGAGAG TAAAGTTTCC 
AAGCAGCTGA AGAACAAGAA GTACTTCTCT GTAGAAAGGA TTCAGCGCAA AAAGAGAGAT 
ATCACACAAC TTCTCAGTAA ACATAAGCAT ACACTTGTGG AAGATAAAGT AGAGGTTGTA 
CCAAAACAAC CAACTGTTCT TGATCTCTTC ACCAAGTCTT TACATGAGAA GGATGGCTGT 
GAAGTTCTAA GCAGAAAGCT CTTCAAGTTC GGCGATAAAG AGATACTGGC AATTTCTACC 
AAGGTTCAAA ATAAAACAGA AGTTCACTTG GCAACAAACC ATACCGACCC ACTTATTCTT 
CACTGGTCTT TGGCAAAAAA TGCTGGAGAA TGGAAGGCAC CTTCTCCAAA TATATTGCCA 
TCTGGTTCCA CATTGCTGGA CAAGGCGTGT GAAACTGAAT TTACTAAATC TGAATTGGAT 
GGTTTGCATT ACCAGGTTGT TGAGATAGAG CTTGATGATG GAGGATACAA AGGAATGCCA 
TTTGTTCTTC GGTCTGGTGA AACATGGATA AAAAATAATG GTTCTGATTT TTTCCTAGAT 
TTCAGCACCC ATGATGTCAG AAATATTAAG TTAAAGGGCA ATGGTGATGC TGGTAAAGGT 
ACTGCTAAGG CATTGCTGGA GAGAATAGCA GATCTGGAGG AAGATGCCCA GCGATCTCTT 
ATGCACAGAT TCAATATTGC AGCAGATCTA GCTGACCAAG CCAGAGATGC TGGACTTTTG 
GGTATTGTTG GGCTTTTTGT TTGGATTAGA TTCATGGCTA CCAGGCAACT AACATGGAAT 
AAGAACTATA ATGTGAAGCC ACGTGAGATA AGCAAAGCAC AGGATAGGTT TACAGATGAT 
CTTGAGAATA TGTACAAAGC TTATCCACAG TACAGAGAGA TATTAAGAAT GATAATGGCT 
GCTGTTGGTC GCGGAGGTGA AGGTGATGTT GGTCAACGCA TTCGTGATGA GATATTAGTA 
ATACAGAGAA ATAATGACTG CAAAGGTGGA ATGATGGAAG AATGGCACCA GAAATTGCAC 
AACAATACAA GCCCAGATGA TGTAGTGATA TGCCAGGCCT TAATTGATTA TATCAAGAGT 
GACTTTGATA TAAGCGTTTA CTGGGACACC TTGAACAAAA ATGGCATAAC CAAAGAGCGT 
CTCTTGAGCT ATGATCGTGC TATTCATTCA GAACCAAATT TCAGAAGTGA ACAGAAGGCG 
GGTTTACTCC GTGACCTGGG AAATTACATG AGAAGCCTAA AGGCTGTGCA TTCTGGTGCT 
GATCTTGAAT CTGCTATAGC AAGTTGTATG GGATACAAAT CAGAGGGTGA AGGTTTCATG 
GTTGGTGTTC AGATCAATCC AGTGAAGGGT TTACCATCTG GATTTCCGGA GTTGCTTGAA 
TTTGTGCTTG AACATGTTGA GGATAAATCA GCGGAACCAC TTCTTGAGGG GCTATTGGAA 
GCTCGAGTTG AACTGCGCCC TTTGCTTCTT GATTCGCGTG AACGCATGAA AGATCTTATA 
TTTTTGGACA TTGCTCTTGA TTCTACCTTC AGGACAGCAA TTGAAAGGTC ATATGAGGAG 
CTGAATGATG CAGCCCCAGA GAAAATAATG TACTTCATCA GTCTTGTCCT TGAAAATCTT 
GCGCTTTCAA TTGACGACAA TGAAGACATC CTGTATTGTT TAAAGGGATG GAACCAAGCC 
TTGGAAATGG CTAAGCAAAA AGACGACCAA TGGGCGCTCT ATGCTAAAGC ATTTCTTGAC 
AGAAACAGAC TTGCCCTTGC GAGCAAGGGA GAACAATACC ATAATATGAT GCAGCCCTCT 
GCTGAGTATC TTGGCTCGTT ACTCAGCATA GACCAATGGG CAGTCAATAT CTTCACAGAA 
GAAATTATAC GCGGTGGATC AGCTGCTACT CTGTCTGCTC TTCTGAACCG ATTTGATCCT 
GTTTTAAGGA ATGTTGCTCA CCTCGGAAGT TGGCAGGTTA TAAGCCCGGT TGAAGTATCA 
GGTTATGTGG TTGTGGTTGA TGAGTTACTT GCTGTCCAGA ACAAATCTTA TGATAAACCA 
ACCATCCTTG TGGCAAAGAG TGTCAAGGGA GAGGAAGAAA TACCAGATGG AGTAGTTGGT 
GTAATTACAC CTGATATGCC AGATGTTCTG TCTCATGTGT CAGTCCGAGC AAGGAATAGC 
AAGGTACTGT TTGCGACCTG TTTTGACCAC ACCACTCTAT CTGAACTTGA AGGATATGAT 
CAGAAACTGT TTTCCTTCAA GCCTACTTCT GCAGATATAA CCTATAGGGA GATCACAGAG 
AGTGAACTTC AGCAATCAAG TTCTCCAAAT GCAGAAGTTG GCCATGCAGT ACCATCTATT 
TCATTGGCCA AGAAGAAATT TCTTGGAAAA TATGCAATAT CAGCCGAAGA ATTCTCTGAG 
GAAATGGTTG GGGCCAAGTC TCGGAATATA GCATACCTCA AAGGAAAAGT ACCTTCATGG 
GTCGGTGTCC CAACGTCAGT TGCGATACCA TTTGGCACTT TTGAGAAGGT TTTGTCAGAT 
GGGCTTAATA AGGAAGTAGC ACAGAGCATA GAGAAGCTTA AGATCAGACT TGCCCAAGAA 
GATTTTAGTG CTCTAGGTGA AATAAGAAAA GTCGTCCTTA ATCTTACTGC TCCTATGCAA 
TTGGTTAATG AGCTGAAGGA GAGGATGCTA GGCTCTGGAA TGCCCTGGCC TGGTGATGAA 
GGAGACAAGC GTTGGGAGCA AGCATGGATG GCTATTAAAA AGGTTTGGGC ATCAAAATGG 
AACGAAAGAG CATATTTTAG CACACGCAAG GTGAAACTTG ATCATGAGTA CCTTTCGATG 
GCTGTTCTCG TGCAAGAAGT TGTGAATGCA GATTATGCTT TTGTCATTCA TACCACAAAC 
CCATCGTCTG GAGATTCTTC TGAGATATAT GCTGAAGTGG TGAAAGGGCT TGGCGAGACC 
CTCGTGGGAG CCTATCCTGG TCGTGCTATG AGCTTTGTTT GCAAAAAAGA TGACCTTGAC 
TCTCCCAAGT TACTTGGTTA CCCAAGCAAG CCAATTGGTC TCTTCATAAG GCAATCAATC 
ATCTTCCGTT CCGACTCCAA CGGTGAGGAC CTGGAAGGTT ATGCTGGAGC AGGATTATAT 
GATAGTGTAC CGATGGATGA GGAGGATGAG GTTGTACTTG ATTATACAAC TGACCCTCTT 
ATAGTAGACC GTGGATTCCG AAGCTCAATC CTCTCAAGCA TAGCACGGGC TGGCCATGCC 
ATCGAGGAGC TATATGGTTC TCCTCAGGAC GTCGAGGGAG TAGTGAAGGA TGGAAAAATC 
TATGTAGTCC AGACAAGACC ACAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020394628.1
CDS	299..4708
Translation	MSGFSAAANAAAAERCALAFRARPAASSPAKRQQQPQPASLRRSGGQRRPTTLSASSRGPVVPRAVATSADRASPDLIGKFTLDSNSELQVAVNPAPQGLVSEISLEVTNTSGSLILHWGALRPDKRDWILPSRKPDGTTVYKNRALRTPFVKSGDNSTLRIEIDDPGVHAIEFLIFDETQNKWFKNNGQNFQVQFQSSRHQGTGASGASSSATSTLVPEDLVQIQAYLRWERRGKQSYTPEQEKEEYEAARAELIEEVNRGVSLEKLRAKLTKAPEAPESDESKSSASRMPIGKLPEDLVQVQAYIRWEQAGKPNYPPEKQLVEFEEARKELQAEVDKGISIDQLRQKILKGNIESKVSKQLKNKKYFSVERIQRKKRDITQLLSKHKHTLVEDKVEVVPKQPTVLDLFTKSLHEKDGCEVLSRKLFKFGDKEILAISTKVQNKTEVHLATNHTDPLILHWSLAKNAGEWKAPSPNILPSGSTLLDKACETEFTKSELDGLHYQVVEIELDDGGYKGMPFVLRSGETWIKNNGSDFFLDFSTHDVRNIKLKGNGDAGKGTAKALLERIADLEEDAQRSLMHRFNIAADLADQARDAGLLGIVGLFVWIRFMATRQLTWNKNYNVKPREISKAQDRFTDDLENMYKAYPQYREILRMIMAAVGRGGEGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNNTSPDDVVICQALIDYIKSDFDISVYWDTLNKNGITKERLLSYDRAIHSEPNFRSEQKAGLLRDLGNYMRSLKAVHSGADLESAIASCMGYKSEGEGFMVGVQINPVKGLPSGFPELLEFVLEHVEDKSAEPLLEGLLEARVELRPLLLDSRERMKDLIFLDIALDSTFRTAIERSYEELNDAAPEKIMYFISLVLENLALSIDDNEDILYCLKGWNQALEMAKQKDDQWALYAKAFLDRNRLALASKGEQYHNMMQPSAEYLGSLLSIDQWAVNIFTEEIIRGGSAATLSALLNRFDPVLRNVAHLGSWQVISPVEVSGYVVVVDELLAVQNKSYDKPTILVAKSVKGEEEIPDGVVGVITPDMPDVLSHVSVRARNSKVLFATCFDHTTLSELEGYDQKLFSFKPTSADITYREITESELQQSSSPNAEVGHAVPSISLAKKKFLGKYAISAEEFSEEMVGAKSRNIAYLKGKVPSWVGVPTSVAIPFGTFEKVLSDGLNKEVAQSIEKLKIRLAQEDFSALGEIRKVVLNLTAPMQLVNELKERMLGSGMPWPGDEGDKRWEQAWMAIKKVWASKWNERAYFSTRKVKLDHEYLSMAVLVQEVVNADYAFVIHTTNPSSGDSSEIYAEVVKGLGETLVGAYPGRAMSFVCKKDDLDSPKLLGYPSKPIGLFIRQSIIFRSDSNGEDLEGYAGAGLYDSVPMDEEDEVVLDYTTDPLIVDRGFRSSILSSIARAGHAIEELYGSPQDVEGVVKDGKIYVVQTRPQM

Target ORF information:

RefSeq Version	XM_020539039.1
Organism	Zea mays
Definition	Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020539039.1

ORF Insert Sequence:

ATGTCCGGAT TCAGTGCCGC GGCCAACGCA GCGGCGGCTG AGCGGTGCGC GCTCGCGTTC 
CGCGCACGGC CCGCGGCCTC CTCGCCAGCG AAGCGGCAGC AGCAGCCGCA GCCAGCGTCC 
CTCCGACGCA GCGGGGGCCA GCGCCGCCCC ACGACGCTCT CCGCCTCTAG CCGCGGCCCC 
GTCGTGCCGC GCGCCGTCGC CACGTCCGCG GACCGCGCGT CCCCCGACCT TATCGGAAAG 
TTCACGCTGG ATTCCAACTC CGAGCTCCAG GTCGCAGTGA ACCCAGCGCC GCAGGGTTTG 
GTGTCAGAGA TTAGCCTGGA GGTGACCAAC ACAAGCGGTT CCCTGATTTT GCATTGGGGA 
GCCCTTCGCC CGGACAAGAG AGATTGGATC CTCCCGTCCA GAAAACCTGA TGGAACGACA 
GTGTACAAGA ACAGGGCTCT CAGGACACCT TTTGTAAAGT CAGGTGATAA CTCCACTCTA 
AGGATTGAGA TAGATGATCC TGGGGTGCAC GCCATTGAGT TCCTCATCTT TGACGAGACA 
CAGAACAAAT GGTTTAAAAA CAATGGCCAG AATTTTCAGG TTCAGTTCCA GTCGAGCCGC 
CATCAGGGTA CTGGTGCATC TGGTGCCTCC TCTTCTGCTA CTTCTACCTT GGTGCCAGAG 
GATCTTGTGC AGATCCAAGC TTACCTTCGG TGGGAAAGAA GGGGAAAGCA GTCATACACA 
CCAGAGCAAG AAAAGGAGGA GTATGAAGCT GCACGAGCTG AGTTAATAGA GGAAGTAAAC 
AGAGGTGTTT CTTTAGAGAA GCTTCGAGCT AAATTGACAA AAGCACCTGA AGCACCTGAG 
TCGGATGAAA GTAAATCTTC TGCATCTCGA ATGCCCATCG GTAAACTTCC AGAGGATCTT 
GTACAGGTGC AGGCTTATAT AAGGTGGGAG CAAGCGGGCA AGCCAAACTA TCCTCCTGAG 
AAGCAACTGG TAGAATTTGA GGAAGCAAGG AAGGAACTGC AGGCTGAGGT GGACAAGGGA 
ATCTCTATTG ATCAGTTGAG GCAGAAAATT TTGAAAGGAA ACATTGAGAG TAAAGTTTCC 
AAGCAGCTGA AGAACAAGAA GTACTTCTCT GTAGAAAGGA TTCAGCGCAA AAAGAGAGAT 
ATCACACAAC TTCTCAGTAA ACATAAGCAT ACACTTGTGG AAGATAAAGT AGAGGTTGTA 
CCAAAACAAC CAACTGTTCT TGATCTCTTC ACCAAGTCTT TACATGAGAA GGATGGCTGT 
GAAGTTCTAA GCAGAAAGCT CTTCAAGTTC GGCGATAAAG AGATACTGGC AATTTCTACC 
AAGGTTCAAA ATAAAACAGA AGTTCACTTG GCAACAAACC ATACCGACCC ACTTATTCTT 
CACTGGTCTT TGGCAAAAAA TGCTGGAGAA TGGAAGGCAC CTTCTCCAAA TATATTGCCA 
TCTGGTTCCA CATTGCTGGA CAAGGCGTGT GAAACTGAAT TTACTAAATC TGAATTGGAT 
GGTTTGCATT ACCAGGTTGT TGAGATAGAG CTTGATGATG GAGGATACAA AGGAATGCCA 
TTTGTTCTTC GGTCTGGTGA AACATGGATA AAAAATAATG GTTCTGATTT TTTCCTAGAT 
TTCAGCACCC ATGATGTCAG AAATATTAAG TTAAAGGGCA ATGGTGATGC TGGTAAAGGT 
ACTGCTAAGG CATTGCTGGA GAGAATAGCA GATCTGGAGG AAGATGCCCA GCGATCTCTT 
ATGCACAGAT TCAATATTGC AGCAGATCTA GCTGACCAAG CCAGAGATGC TGGACTTTTG 
GGTATTGTTG GGCTTTTTGT TTGGATTAGA TTCATGGCTA CCAGGCAACT AACATGGAAT 
AAGAACTATA ATGTGAAGCC ACGTGAGATA AGCAAAGCAC AGGATAGGTT TACAGATGAT 
CTTGAGAATA TGTACAAAGC TTATCCACAG TACAGAGAGA TATTAAGAAT GATAATGGCT 
GCTGTTGGTC GCGGAGGTGA AGGTGATGTT GGTCAACGCA TTCGTGATGA GATATTAGTA 
ATACAGAGAA ATAATGACTG CAAAGGTGGA ATGATGGAAG AATGGCACCA GAAATTGCAC 
AACAATACAA GCCCAGATGA TGTAGTGATA TGCCAGGCCT TAATTGATTA TATCAAGAGT 
GACTTTGATA TAAGCGTTTA CTGGGACACC TTGAACAAAA ATGGCATAAC CAAAGAGCGT 
CTCTTGAGCT ATGATCGTGC TATTCATTCA GAACCAAATT TCAGAAGTGA ACAGAAGGCG 
GGTTTACTCC GTGACCTGGG AAATTACATG AGAAGCCTAA AGGCTGTGCA TTCTGGTGCT 
GATCTTGAAT CTGCTATAGC AAGTTGTATG GGATACAAAT CAGAGGGTGA AGGTTTCATG 
GTTGGTGTTC AGATCAATCC AGTGAAGGGT TTACCATCTG GATTTCCGGA GTTGCTTGAA 
TTTGTGCTTG AACATGTTGA GGATAAATCA GCGGAACCAC TTCTTGAGGG GCTATTGGAA 
GCTCGAGTTG AACTGCGCCC TTTGCTTCTT GATTCGCGTG AACGCATGAA AGATCTTATA 
TTTTTGGACA TTGCTCTTGA TTCTACCTTC AGGACAGCAA TTGAAAGGTC ATATGAGGAG 
CTGAATGATG CAGCCCCAGA GAAAATAATG TACTTCATCA GTCTTGTCCT TGAAAATCTT 
GCGCTTTCAA TTGACGACAA TGAAGACATC CTGTATTGTT TAAAGGGATG GAACCAAGCC 
TTGGAAATGG CTAAGCAAAA AGACGACCAA TGGGCGCTCT ATGCTAAAGC ATTTCTTGAC 
AGAAACAGAC TTGCCCTTGC GAGCAAGGGA GAACAATACC ATAATATGAT GCAGCCCTCT 
GCTGAGTATC TTGGCTCGTT ACTCAGCATA GACCAATGGG CAGTCAATAT CTTCACAGAA 
GAAATTATAC GCGGTGGATC AGCTGCTACT CTGTCTGCTC TTCTGAACCG ATTTGATCCT 
GTTTTAAGGA ATGTTGCTCA CCTCGGAAGT TGGCAGGTTA TAAGCCCGGT TGAAGTATCA 
GGTTATGTGG TTGTGGTTGA TGAGTTACTT GCTGTCCAGA ACAAATCTTA TGATAAACCA 
ACCATCCTTG TGGCAAAGAG TGTCAAGGGA GAGGAAGAAA TACCAGATGG AGTAGTTGGT 
GTAATTACAC CTGATATGCC AGATGTTCTG TCTCATGTGT CAGTCCGAGC AAGGAATAGC 
AAGGTACTGT TTGCGACCTG TTTTGACCAC ACCACTCTAT CTGAACTTGA AGGATATGAT 
CAGAAACTGT TTTCCTTCAA GCCTACTTCT GCAGATATAA CCTATAGGGA GATCACAGAG 
AGTGAACTTC AGCAATCAAG TTCTCCAAAT GCAGAAGTTG GCCATGCAGT ACCATCTATT 
TCATTGGCCA AGAAGAAATT TCTTGGAAAA TATGCAATAT CAGCCGAAGA ATTCTCTGAG 
GAAATGGTTG GGGCCAAGTC TCGGAATATA GCATACCTCA AAGGAAAAGT ACCTTCATGG 
GTCGGTGTCC CAACGTCAGT TGCGATACCA TTTGGCACTT TTGAGAAGGT TTTGTCAGAT 
GGGCTTAATA AGGAAGTAGC ACAGAGCATA GAGAAGCTTA AGATCAGACT TGCCCAAGAA 
GATTTTAGTG CTCTAGGTGA AATAAGAAAA GTCGTCCTTA ATCTTACTGC TCCTATGCAA 
TTGGTTAATG AGCTGAAGGA GAGGATGCTA GGCTCTGGAA TGCCCTGGCC TGGTGATGAA 
GGAGACAAGC GTTGGGAGCA AGCATGGATG GCTATTAAAA AGGTTTGGGC ATCAAAATGG 
AACGAAAGAG CATATTTTAG CACACGCAAG GTGAAACTTG ATCATGAGTA CCTTTCGATG 
GCTGTTCTCG TGCAAGAAGT TGTGAATGCA GATTATGCTT TTGTCATTCA TACCACAAAC 
CCATCGTCTG GAGATTCTTC TGAGATATAT GCTGAAGTGG TGAAAGGGCT TGGCGAGACC 
CTCGTGGGAG CCTATCCTGG TCGTGCTATG AGCTTTGTTT GCAAAAAAGA TGACCTTGAC 
TCTCCCAAGT TACTTGGTTA CCCAAGCAAG CCAATTGGTC TCTTCATAAG GCAATCAATC 
ATCTTCCGTT CCGACTCCAA CGGTGAGGAC CTGGAAGGTT ATGCTGGAGC AGGATTATAT 
GATAGTGTAC CGATGGATGA GGAGGATGAG GTTGTACTTG ATTATACAAC TGACCCTCTT 
ATAGTAGACC GTGGATTCCG AAGCTCAATC CTCTCAAGCA TAGCACGGGC TGGCCATGCC 
ATCGAGGAGC TATATGGTTC TCCTCAGGAC GTCGAGGGAG TAGTGAAGGA TGGAAAAATC 
TATGTAGTCC AGACAAGACC ACAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AY109804 ( XM_020539038.1 ) cDNA ORF clone, Zea mays -> XP_020394627.1 Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X2, mRNA.

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CloneID	OZa29821
Clone ID Related Accession (Same CDS sequence)	XM_008649791.2 , XM_020539039.1 , XM_020539038.1 , NM_001361424.1 , XM_020539039.2
Accession Version	XM_020539038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1489852800000
Organism	Zea mays
Product	alpha-glucan water dikinase 1 chloroplastic isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024464.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAT TCAGTGCCGC GGCCAACGCA GCGGCGGCTG AGCGGTGCGC GCTCGCGTTC 
CGCGCACGGC CCGCGGCCTC CTCGCCAGCG AAGCGGCAGC AGCAGCCGCA GCCAGCGTCC 
CTCCGACGCA GCGGGGGCCA GCGCCGCCCC ACGACGCTCT CCGCCTCTAG CCGCGGCCCC 
GTCGTGCCGC GCGCCGTCGC CACGTCCGCG GACCGCGCGT CCCCCGACCT TATCGGAAAG 
TTCACGCTGG ATTCCAACTC CGAGCTCCAG GTCGCAGTGA ACCCAGCGCC GCAGGGTTTG 
GTGTCAGAGA TTAGCCTGGA GGTGACCAAC ACAAGCGGTT CCCTGATTTT GCATTGGGGA 
GCCCTTCGCC CGGACAAGAG AGATTGGATC CTCCCGTCCA GAAAACCTGA TGGAACGACA 
GTGTACAAGA ACAGGGCTCT CAGGACACCT TTTGTAAAGT CAGGTGATAA CTCCACTCTA 
AGGATTGAGA TAGATGATCC TGGGGTGCAC GCCATTGAGT TCCTCATCTT TGACGAGACA 
CAGAACAAAT GGTTTAAAAA CAATGGCCAG AATTTTCAGG TTCAGTTCCA GTCGAGCCGC 
CATCAGGGTA CTGGTGCATC TGGTGCCTCC TCTTCTGCTA CTTCTACCTT GGTGCCAGAG 
GATCTTGTGC AGATCCAAGC TTACCTTCGG TGGGAAAGAA GGGGAAAGCA GTCATACACA 
CCAGAGCAAG AAAAGGAGGA GTATGAAGCT GCACGAGCTG AGTTAATAGA GGAAGTAAAC 
AGAGGTGTTT CTTTAGAGAA GCTTCGAGCT AAATTGACAA AAGCACCTGA AGCACCTGAG 
TCGGATGAAA GTAAATCTTC TGCATCTCGA ATGCCCATCG GTAAACTTCC AGAGGATCTT 
GTACAGGTGC AGGCTTATAT AAGGTGGGAG CAAGCGGGCA AGCCAAACTA TCCTCCTGAG 
AAGCAACTGG TAGAATTTGA GGAAGCAAGG AAGGAACTGC AGGCTGAGGT GGACAAGGGA 
ATCTCTATTG ATCAGTTGAG GCAGAAAATT TTGAAAGGAA ACATTGAGAG TAAAGTTTCC 
AAGCAGCTGA AGAACAAGAA GTACTTCTCT GTAGAAAGGA TTCAGCGCAA AAAGAGAGAT 
ATCACACAAC TTCTCAGTAA ACATAAGCAT ACACTTGTGG AAGATAAAGT AGAGGTTGTA 
CCAAAACAAC CAACTGTTCT TGATCTCTTC ACCAAGTCTT TACATGAGAA GGATGGCTGT 
GAAGTTCTAA GCAGAAAGCT CTTCAAGTTC GGCGATAAAG AGATACTGGC AATTTCTACC 
AAGGTTCAAA ATAAAACAGA AGTTCACTTG GCAACAAACC ATACCGACCC ACTTATTCTT 
CACTGGTCTT TGGCAAAAAA TGCTGGAGAA TGGAAGGCAC CTTCTCCAAA TATATTGCCA 
TCTGGTTCCA CATTGCTGGA CAAGGCGTGT GAAACTGAAT TTACTAAATC TGAATTGGAT 
GGTTTGCATT ACCAGGTTGT TGAGATAGAG CTTGATGATG GAGGATACAA AGGAATGCCA 
TTTGTTCTTC GGTCTGGTGA AACATGGATA AAAAATAATG GTTCTGATTT TTTCCTAGAT 
TTCAGCACCC ATGATGTCAG AAATATTAAG TTAAAGGGCA ATGGTGATGC TGGTAAAGGT 
ACTGCTAAGG CATTGCTGGA GAGAATAGCA GATCTGGAGG AAGATGCCCA GCGATCTCTT 
ATGCACAGAT TCAATATTGC AGCAGATCTA GCTGACCAAG CCAGAGATGC TGGACTTTTG 
GGTATTGTTG GGCTTTTTGT TTGGATTAGA TTCATGGCTA CCAGGCAACT AACATGGAAT 
AAGAACTATA ATGTGAAGCC ACGTGAGATA AGCAAAGCAC AGGATAGGTT TACAGATGAT 
CTTGAGAATA TGTACAAAGC TTATCCACAG TACAGAGAGA TATTAAGAAT GATAATGGCT 
GCTGTTGGTC GCGGAGGTGA AGGTGATGTT GGTCAACGCA TTCGTGATGA GATATTAGTA 
ATACAGAGAA ATAATGACTG CAAAGGTGGA ATGATGGAAG AATGGCACCA GAAATTGCAC 
AACAATACAA GCCCAGATGA TGTAGTGATA TGCCAGGCCT TAATTGATTA TATCAAGAGT 
GACTTTGATA TAAGCGTTTA CTGGGACACC TTGAACAAAA ATGGCATAAC CAAAGAGCGT 
CTCTTGAGCT ATGATCGTGC TATTCATTCA GAACCAAATT TCAGAAGTGA ACAGAAGGCG 
GGTTTACTCC GTGACCTGGG AAATTACATG AGAAGCCTAA AGGCTGTGCA TTCTGGTGCT 
GATCTTGAAT CTGCTATAGC AAGTTGTATG GGATACAAAT CAGAGGGTGA AGGTTTCATG 
GTTGGTGTTC AGATCAATCC AGTGAAGGGT TTACCATCTG GATTTCCGGA GTTGCTTGAA 
TTTGTGCTTG AACATGTTGA GGATAAATCA GCGGAACCAC TTCTTGAGGG GCTATTGGAA 
GCTCGAGTTG AACTGCGCCC TTTGCTTCTT GATTCGCGTG AACGCATGAA AGATCTTATA 
TTTTTGGACA TTGCTCTTGA TTCTACCTTC AGGACAGCAA TTGAAAGGTC ATATGAGGAG 
CTGAATGATG CAGCCCCAGA GAAAATAATG TACTTCATCA GTCTTGTCCT TGAAAATCTT 
GCGCTTTCAA TTGACGACAA TGAAGACATC CTGTATTGTT TAAAGGGATG GAACCAAGCC 
TTGGAAATGG CTAAGCAAAA AGACGACCAA TGGGCGCTCT ATGCTAAAGC ATTTCTTGAC 
AGAAACAGAC TTGCCCTTGC GAGCAAGGGA GAACAATACC ATAATATGAT GCAGCCCTCT 
GCTGAGTATC TTGGCTCGTT ACTCAGCATA GACCAATGGG CAGTCAATAT CTTCACAGAA 
GAAATTATAC GCGGTGGATC AGCTGCTACT CTGTCTGCTC TTCTGAACCG ATTTGATCCT 
GTTTTAAGGA ATGTTGCTCA CCTCGGAAGT TGGCAGGTTA TAAGCCCGGT TGAAGTATCA 
GGTTATGTGG TTGTGGTTGA TGAGTTACTT GCTGTCCAGA ACAAATCTTA TGATAAACCA 
ACCATCCTTG TGGCAAAGAG TGTCAAGGGA GAGGAAGAAA TACCAGATGG AGTAGTTGGT 
GTAATTACAC CTGATATGCC AGATGTTCTG TCTCATGTGT CAGTCCGAGC AAGGAATAGC 
AAGGTACTGT TTGCGACCTG TTTTGACCAC ACCACTCTAT CTGAACTTGA AGGATATGAT 
CAGAAACTGT TTTCCTTCAA GCCTACTTCT GCAGATATAA CCTATAGGGA GATCACAGAG 
AGTGAACTTC AGCAATCAAG TTCTCCAAAT GCAGAAGTTG GCCATGCAGT ACCATCTATT 
TCATTGGCCA AGAAGAAATT TCTTGGAAAA TATGCAATAT CAGCCGAAGA ATTCTCTGAG 
GAAATGGTTG GGGCCAAGTC TCGGAATATA GCATACCTCA AAGGAAAAGT ACCTTCATGG 
GTCGGTGTCC CAACGTCAGT TGCGATACCA TTTGGCACTT TTGAGAAGGT TTTGTCAGAT 
GGGCTTAATA AGGAAGTAGC ACAGAGCATA GAGAAGCTTA AGATCAGACT TGCCCAAGAA 
GATTTTAGTG CTCTAGGTGA AATAAGAAAA GTCGTCCTTA ATCTTACTGC TCCTATGCAA 
TTGGTTAATG AGCTGAAGGA GAGGATGCTA GGCTCTGGAA TGCCCTGGCC TGGTGATGAA 
GGAGACAAGC GTTGGGAGCA AGCATGGATG GCTATTAAAA AGGTTTGGGC ATCAAAATGG 
AACGAAAGAG CATATTTTAG CACACGCAAG GTGAAACTTG ATCATGAGTA CCTTTCGATG 
GCTGTTCTCG TGCAAGAAGT TGTGAATGCA GATTATGCTT TTGTCATTCA TACCACAAAC 
CCATCGTCTG GAGATTCTTC TGAGATATAT GCTGAAGTGG TGAAAGGGCT TGGCGAGACC 
CTCGTGGGAG CCTATCCTGG TCGTGCTATG AGCTTTGTTT GCAAAAAAGA TGACCTTGAC 
TCTCCCAAGT TACTTGGTTA CCCAAGCAAG CCAATTGGTC TCTTCATAAG GCAATCAATC 
ATCTTCCGTT CCGACTCCAA CGGTGAGGAC CTGGAAGGTT ATGCTGGAGC AGGATTATAT 
GATAGTGTAC CGATGGATGA GGAGGATGAG GTTGTACTTG ATTATACAAC TGACCCTCTT 
ATAGTAGACC GTGGATTCCG AAGCTCAATC CTCTCAAGCA TAGCACGGGC TGGCCATGCC 
ATCGAGGAGC TATATGGTTC TCCTCAGGAC GTCGAGGGAG TAGTGAAGGA TGGAAAAATC 
TATGTAGTCC AGACAAGACC ACAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020394627.1
CDS	435..4844
Translation	MSGFSAAANAAAAERCALAFRARPAASSPAKRQQQPQPASLRRSGGQRRPTTLSASSRGPVVPRAVATSADRASPDLIGKFTLDSNSELQVAVNPAPQGLVSEISLEVTNTSGSLILHWGALRPDKRDWILPSRKPDGTTVYKNRALRTPFVKSGDNSTLRIEIDDPGVHAIEFLIFDETQNKWFKNNGQNFQVQFQSSRHQGTGASGASSSATSTLVPEDLVQIQAYLRWERRGKQSYTPEQEKEEYEAARAELIEEVNRGVSLEKLRAKLTKAPEAPESDESKSSASRMPIGKLPEDLVQVQAYIRWEQAGKPNYPPEKQLVEFEEARKELQAEVDKGISIDQLRQKILKGNIESKVSKQLKNKKYFSVERIQRKKRDITQLLSKHKHTLVEDKVEVVPKQPTVLDLFTKSLHEKDGCEVLSRKLFKFGDKEILAISTKVQNKTEVHLATNHTDPLILHWSLAKNAGEWKAPSPNILPSGSTLLDKACETEFTKSELDGLHYQVVEIELDDGGYKGMPFVLRSGETWIKNNGSDFFLDFSTHDVRNIKLKGNGDAGKGTAKALLERIADLEEDAQRSLMHRFNIAADLADQARDAGLLGIVGLFVWIRFMATRQLTWNKNYNVKPREISKAQDRFTDDLENMYKAYPQYREILRMIMAAVGRGGEGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNNTSPDDVVICQALIDYIKSDFDISVYWDTLNKNGITKERLLSYDRAIHSEPNFRSEQKAGLLRDLGNYMRSLKAVHSGADLESAIASCMGYKSEGEGFMVGVQINPVKGLPSGFPELLEFVLEHVEDKSAEPLLEGLLEARVELRPLLLDSRERMKDLIFLDIALDSTFRTAIERSYEELNDAAPEKIMYFISLVLENLALSIDDNEDILYCLKGWNQALEMAKQKDDQWALYAKAFLDRNRLALASKGEQYHNMMQPSAEYLGSLLSIDQWAVNIFTEEIIRGGSAATLSALLNRFDPVLRNVAHLGSWQVISPVEVSGYVVVVDELLAVQNKSYDKPTILVAKSVKGEEEIPDGVVGVITPDMPDVLSHVSVRARNSKVLFATCFDHTTLSELEGYDQKLFSFKPTSADITYREITESELQQSSSPNAEVGHAVPSISLAKKKFLGKYAISAEEFSEEMVGAKSRNIAYLKGKVPSWVGVPTSVAIPFGTFEKVLSDGLNKEVAQSIEKLKIRLAQEDFSALGEIRKVVLNLTAPMQLVNELKERMLGSGMPWPGDEGDKRWEQAWMAIKKVWASKWNERAYFSTRKVKLDHEYLSMAVLVQEVVNADYAFVIHTTNPSSGDSSEIYAEVVKGLGETLVGAYPGRAMSFVCKKDDLDSPKLLGYPSKPIGLFIRQSIIFRSDSNGEDLEGYAGAGLYDSVPMDEEDEVVLDYTTDPLIVDRGFRSSILSSIARAGHAIEELYGSPQDVEGVVKDGKIYVVQTRPQM

Target ORF information:

RefSeq Version	XM_020539038.1
Organism	Zea mays
Definition	Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020539038.1

ORF Insert Sequence:

ATGTCCGGAT TCAGTGCCGC GGCCAACGCA GCGGCGGCTG AGCGGTGCGC GCTCGCGTTC 
CGCGCACGGC CCGCGGCCTC CTCGCCAGCG AAGCGGCAGC AGCAGCCGCA GCCAGCGTCC 
CTCCGACGCA GCGGGGGCCA GCGCCGCCCC ACGACGCTCT CCGCCTCTAG CCGCGGCCCC 
GTCGTGCCGC GCGCCGTCGC CACGTCCGCG GACCGCGCGT CCCCCGACCT TATCGGAAAG 
TTCACGCTGG ATTCCAACTC CGAGCTCCAG GTCGCAGTGA ACCCAGCGCC GCAGGGTTTG 
GTGTCAGAGA TTAGCCTGGA GGTGACCAAC ACAAGCGGTT CCCTGATTTT GCATTGGGGA 
GCCCTTCGCC CGGACAAGAG AGATTGGATC CTCCCGTCCA GAAAACCTGA TGGAACGACA 
GTGTACAAGA ACAGGGCTCT CAGGACACCT TTTGTAAAGT CAGGTGATAA CTCCACTCTA 
AGGATTGAGA TAGATGATCC TGGGGTGCAC GCCATTGAGT TCCTCATCTT TGACGAGACA 
CAGAACAAAT GGTTTAAAAA CAATGGCCAG AATTTTCAGG TTCAGTTCCA GTCGAGCCGC 
CATCAGGGTA CTGGTGCATC TGGTGCCTCC TCTTCTGCTA CTTCTACCTT GGTGCCAGAG 
GATCTTGTGC AGATCCAAGC TTACCTTCGG TGGGAAAGAA GGGGAAAGCA GTCATACACA 
CCAGAGCAAG AAAAGGAGGA GTATGAAGCT GCACGAGCTG AGTTAATAGA GGAAGTAAAC 
AGAGGTGTTT CTTTAGAGAA GCTTCGAGCT AAATTGACAA AAGCACCTGA AGCACCTGAG 
TCGGATGAAA GTAAATCTTC TGCATCTCGA ATGCCCATCG GTAAACTTCC AGAGGATCTT 
GTACAGGTGC AGGCTTATAT AAGGTGGGAG CAAGCGGGCA AGCCAAACTA TCCTCCTGAG 
AAGCAACTGG TAGAATTTGA GGAAGCAAGG AAGGAACTGC AGGCTGAGGT GGACAAGGGA 
ATCTCTATTG ATCAGTTGAG GCAGAAAATT TTGAAAGGAA ACATTGAGAG TAAAGTTTCC 
AAGCAGCTGA AGAACAAGAA GTACTTCTCT GTAGAAAGGA TTCAGCGCAA AAAGAGAGAT 
ATCACACAAC TTCTCAGTAA ACATAAGCAT ACACTTGTGG AAGATAAAGT AGAGGTTGTA 
CCAAAACAAC CAACTGTTCT TGATCTCTTC ACCAAGTCTT TACATGAGAA GGATGGCTGT 
GAAGTTCTAA GCAGAAAGCT CTTCAAGTTC GGCGATAAAG AGATACTGGC AATTTCTACC 
AAGGTTCAAA ATAAAACAGA AGTTCACTTG GCAACAAACC ATACCGACCC ACTTATTCTT 
CACTGGTCTT TGGCAAAAAA TGCTGGAGAA TGGAAGGCAC CTTCTCCAAA TATATTGCCA 
TCTGGTTCCA CATTGCTGGA CAAGGCGTGT GAAACTGAAT TTACTAAATC TGAATTGGAT 
GGTTTGCATT ACCAGGTTGT TGAGATAGAG CTTGATGATG GAGGATACAA AGGAATGCCA 
TTTGTTCTTC GGTCTGGTGA AACATGGATA AAAAATAATG GTTCTGATTT TTTCCTAGAT 
TTCAGCACCC ATGATGTCAG AAATATTAAG TTAAAGGGCA ATGGTGATGC TGGTAAAGGT 
ACTGCTAAGG CATTGCTGGA GAGAATAGCA GATCTGGAGG AAGATGCCCA GCGATCTCTT 
ATGCACAGAT TCAATATTGC AGCAGATCTA GCTGACCAAG CCAGAGATGC TGGACTTTTG 
GGTATTGTTG GGCTTTTTGT TTGGATTAGA TTCATGGCTA CCAGGCAACT AACATGGAAT 
AAGAACTATA ATGTGAAGCC ACGTGAGATA AGCAAAGCAC AGGATAGGTT TACAGATGAT 
CTTGAGAATA TGTACAAAGC TTATCCACAG TACAGAGAGA TATTAAGAAT GATAATGGCT 
GCTGTTGGTC GCGGAGGTGA AGGTGATGTT GGTCAACGCA TTCGTGATGA GATATTAGTA 
ATACAGAGAA ATAATGACTG CAAAGGTGGA ATGATGGAAG AATGGCACCA GAAATTGCAC 
AACAATACAA GCCCAGATGA TGTAGTGATA TGCCAGGCCT TAATTGATTA TATCAAGAGT 
GACTTTGATA TAAGCGTTTA CTGGGACACC TTGAACAAAA ATGGCATAAC CAAAGAGCGT 
CTCTTGAGCT ATGATCGTGC TATTCATTCA GAACCAAATT TCAGAAGTGA ACAGAAGGCG 
GGTTTACTCC GTGACCTGGG AAATTACATG AGAAGCCTAA AGGCTGTGCA TTCTGGTGCT 
GATCTTGAAT CTGCTATAGC AAGTTGTATG GGATACAAAT CAGAGGGTGA AGGTTTCATG 
GTTGGTGTTC AGATCAATCC AGTGAAGGGT TTACCATCTG GATTTCCGGA GTTGCTTGAA 
TTTGTGCTTG AACATGTTGA GGATAAATCA GCGGAACCAC TTCTTGAGGG GCTATTGGAA 
GCTCGAGTTG AACTGCGCCC TTTGCTTCTT GATTCGCGTG AACGCATGAA AGATCTTATA 
TTTTTGGACA TTGCTCTTGA TTCTACCTTC AGGACAGCAA TTGAAAGGTC ATATGAGGAG 
CTGAATGATG CAGCCCCAGA GAAAATAATG TACTTCATCA GTCTTGTCCT TGAAAATCTT 
GCGCTTTCAA TTGACGACAA TGAAGACATC CTGTATTGTT TAAAGGGATG GAACCAAGCC 
TTGGAAATGG CTAAGCAAAA AGACGACCAA TGGGCGCTCT ATGCTAAAGC ATTTCTTGAC 
AGAAACAGAC TTGCCCTTGC GAGCAAGGGA GAACAATACC ATAATATGAT GCAGCCCTCT 
GCTGAGTATC TTGGCTCGTT ACTCAGCATA GACCAATGGG CAGTCAATAT CTTCACAGAA 
GAAATTATAC GCGGTGGATC AGCTGCTACT CTGTCTGCTC TTCTGAACCG ATTTGATCCT 
GTTTTAAGGA ATGTTGCTCA CCTCGGAAGT TGGCAGGTTA TAAGCCCGGT TGAAGTATCA 
GGTTATGTGG TTGTGGTTGA TGAGTTACTT GCTGTCCAGA ACAAATCTTA TGATAAACCA 
ACCATCCTTG TGGCAAAGAG TGTCAAGGGA GAGGAAGAAA TACCAGATGG AGTAGTTGGT 
GTAATTACAC CTGATATGCC AGATGTTCTG TCTCATGTGT CAGTCCGAGC AAGGAATAGC 
AAGGTACTGT TTGCGACCTG TTTTGACCAC ACCACTCTAT CTGAACTTGA AGGATATGAT 
CAGAAACTGT TTTCCTTCAA GCCTACTTCT GCAGATATAA CCTATAGGGA GATCACAGAG 
AGTGAACTTC AGCAATCAAG TTCTCCAAAT GCAGAAGTTG GCCATGCAGT ACCATCTATT 
TCATTGGCCA AGAAGAAATT TCTTGGAAAA TATGCAATAT CAGCCGAAGA ATTCTCTGAG 
GAAATGGTTG GGGCCAAGTC TCGGAATATA GCATACCTCA AAGGAAAAGT ACCTTCATGG 
GTCGGTGTCC CAACGTCAGT TGCGATACCA TTTGGCACTT TTGAGAAGGT TTTGTCAGAT 
GGGCTTAATA AGGAAGTAGC ACAGAGCATA GAGAAGCTTA AGATCAGACT TGCCCAAGAA 
GATTTTAGTG CTCTAGGTGA AATAAGAAAA GTCGTCCTTA ATCTTACTGC TCCTATGCAA 
TTGGTTAATG AGCTGAAGGA GAGGATGCTA GGCTCTGGAA TGCCCTGGCC TGGTGATGAA 
GGAGACAAGC GTTGGGAGCA AGCATGGATG GCTATTAAAA AGGTTTGGGC ATCAAAATGG 
AACGAAAGAG CATATTTTAG CACACGCAAG GTGAAACTTG ATCATGAGTA CCTTTCGATG 
GCTGTTCTCG TGCAAGAAGT TGTGAATGCA GATTATGCTT TTGTCATTCA TACCACAAAC 
CCATCGTCTG GAGATTCTTC TGAGATATAT GCTGAAGTGG TGAAAGGGCT TGGCGAGACC 
CTCGTGGGAG CCTATCCTGG TCGTGCTATG AGCTTTGTTT GCAAAAAAGA TGACCTTGAC 
TCTCCCAAGT TACTTGGTTA CCCAAGCAAG CCAATTGGTC TCTTCATAAG GCAATCAATC 
ATCTTCCGTT CCGACTCCAA CGGTGAGGAC CTGGAAGGTT ATGCTGGAGC AGGATTATAT 
GATAGTGTAC CGATGGATGA GGAGGATGAG GTTGTACTTG ATTATACAAC TGACCCTCTT 
ATAGTAGACC GTGGATTCCG AAGCTCAATC CTCTCAAGCA TAGCACGGGC TGGCCATGCC 
ATCGAGGAGC TATATGGTTC TCCTCAGGAC GTCGAGGGAG TAGTGAAGGA TGGAAAAATC 
TATGTAGTCC AGACAAGACC ACAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AY109804 ( XM_020539039.2 ) cDNA ORF clone, Zea mays -> XP_020394628.1 Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X2, mRNA.

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CloneID	OZa29821
Clone ID Related Accession (Same CDS sequence)	XM_008649791.2 , XM_020539039.1 , XM_020539038.1 , NM_001361424.1 , XM_020539039.2
Accession Version	XM_020539039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	alpha-glucan water dikinase 1 chloroplastic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024464.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2017 this sequence version replaced XM_020539039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAT TCAGTGCCGC GGCCAACGCA GCGGCGGCTG AGCGGTGCGC GCTCGCGTTC 
CGCGCACGGC CCGCGGCCTC CTCGCCAGCG AAGCGGCAGC AGCAGCCGCA GCCAGCGTCC 
CTCCGACGCA GCGGGGGCCA GCGCCGCCCC ACGACGCTCT CCGCCTCTAG CCGCGGCCCC 
GTCGTGCCGC GCGCCGTCGC CACGTCCGCG GACCGCGCGT CCCCCGACCT TATCGGAAAG 
TTCACGCTGG ATTCCAACTC CGAGCTCCAG GTCGCAGTGA ACCCAGCGCC GCAGGGTTTG 
GTGTCAGAGA TTAGCCTGGA GGTGACCAAC ACAAGCGGTT CCCTGATTTT GCATTGGGGA 
GCCCTTCGCC CGGACAAGAG AGATTGGATC CTCCCGTCCA GAAAACCTGA TGGAACGACA 
GTGTACAAGA ACAGGGCTCT CAGGACACCT TTTGTAAAGT CAGGTGATAA CTCCACTCTA 
AGGATTGAGA TAGATGATCC TGGGGTGCAC GCCATTGAGT TCCTCATCTT TGACGAGACA 
CAGAACAAAT GGTTTAAAAA CAATGGCCAG AATTTTCAGG TTCAGTTCCA GTCGAGCCGC 
CATCAGGGTA CTGGTGCATC TGGTGCCTCC TCTTCTGCTA CTTCTACCTT GGTGCCAGAG 
GATCTTGTGC AGATCCAAGC TTACCTTCGG TGGGAAAGAA GGGGAAAGCA GTCATACACA 
CCAGAGCAAG AAAAGGAGGA GTATGAAGCT GCACGAGCTG AGTTAATAGA GGAAGTAAAC 
AGAGGTGTTT CTTTAGAGAA GCTTCGAGCT AAATTGACAA AAGCACCTGA AGCACCTGAG 
TCGGATGAAA GTAAATCTTC TGCATCTCGA ATGCCCATCG GTAAACTTCC AGAGGATCTT 
GTACAGGTGC AGGCTTATAT AAGGTGGGAG CAAGCGGGCA AGCCAAACTA TCCTCCTGAG 
AAGCAACTGG TAGAATTTGA GGAAGCAAGG AAGGAACTGC AGGCTGAGGT GGACAAGGGA 
ATCTCTATTG ATCAGTTGAG GCAGAAAATT TTGAAAGGAA ACATTGAGAG TAAAGTTTCC 
AAGCAGCTGA AGAACAAGAA GTACTTCTCT GTAGAAAGGA TTCAGCGCAA AAAGAGAGAT 
ATCACACAAC TTCTCAGTAA ACATAAGCAT ACACTTGTGG AAGATAAAGT AGAGGTTGTA 
CCAAAACAAC CAACTGTTCT TGATCTCTTC ACCAAGTCTT TACATGAGAA GGATGGCTGT 
GAAGTTCTAA GCAGAAAGCT CTTCAAGTTC GGCGATAAAG AGATACTGGC AATTTCTACC 
AAGGTTCAAA ATAAAACAGA AGTTCACTTG GCAACAAACC ATACCGACCC ACTTATTCTT 
CACTGGTCTT TGGCAAAAAA TGCTGGAGAA TGGAAGGCAC CTTCTCCAAA TATATTGCCA 
TCTGGTTCCA CATTGCTGGA CAAGGCGTGT GAAACTGAAT TTACTAAATC TGAATTGGAT 
GGTTTGCATT ACCAGGTTGT TGAGATAGAG CTTGATGATG GAGGATACAA AGGAATGCCA 
TTTGTTCTTC GGTCTGGTGA AACATGGATA AAAAATAATG GTTCTGATTT TTTCCTAGAT 
TTCAGCACCC ATGATGTCAG AAATATTAAG TTAAAGGGCA ATGGTGATGC TGGTAAAGGT 
ACTGCTAAGG CATTGCTGGA GAGAATAGCA GATCTGGAGG AAGATGCCCA GCGATCTCTT 
ATGCACAGAT TCAATATTGC AGCAGATCTA GCTGACCAAG CCAGAGATGC TGGACTTTTG 
GGTATTGTTG GGCTTTTTGT TTGGATTAGA TTCATGGCTA CCAGGCAACT AACATGGAAT 
AAGAACTATA ATGTGAAGCC ACGTGAGATA AGCAAAGCAC AGGATAGGTT TACAGATGAT 
CTTGAGAATA TGTACAAAGC TTATCCACAG TACAGAGAGA TATTAAGAAT GATAATGGCT 
GCTGTTGGTC GCGGAGGTGA AGGTGATGTT GGTCAACGCA TTCGTGATGA GATATTAGTA 
ATACAGAGAA ATAATGACTG CAAAGGTGGA ATGATGGAAG AATGGCACCA GAAATTGCAC 
AACAATACAA GCCCAGATGA TGTAGTGATA TGCCAGGCCT TAATTGATTA TATCAAGAGT 
GACTTTGATA TAAGCGTTTA CTGGGACACC TTGAACAAAA ATGGCATAAC CAAAGAGCGT 
CTCTTGAGCT ATGATCGTGC TATTCATTCA GAACCAAATT TCAGAAGTGA ACAGAAGGCG 
GGTTTACTCC GTGACCTGGG AAATTACATG AGAAGCCTAA AGGCTGTGCA TTCTGGTGCT 
GATCTTGAAT CTGCTATAGC AAGTTGTATG GGATACAAAT CAGAGGGTGA AGGTTTCATG 
GTTGGTGTTC AGATCAATCC AGTGAAGGGT TTACCATCTG GATTTCCGGA GTTGCTTGAA 
TTTGTGCTTG AACATGTTGA GGATAAATCA GCGGAACCAC TTCTTGAGGG GCTATTGGAA 
GCTCGAGTTG AACTGCGCCC TTTGCTTCTT GATTCGCGTG AACGCATGAA AGATCTTATA 
TTTTTGGACA TTGCTCTTGA TTCTACCTTC AGGACAGCAA TTGAAAGGTC ATATGAGGAG 
CTGAATGATG CAGCCCCAGA GAAAATAATG TACTTCATCA GTCTTGTCCT TGAAAATCTT 
GCGCTTTCAA TTGACGACAA TGAAGACATC CTGTATTGTT TAAAGGGATG GAACCAAGCC 
TTGGAAATGG CTAAGCAAAA AGACGACCAA TGGGCGCTCT ATGCTAAAGC ATTTCTTGAC 
AGAAACAGAC TTGCCCTTGC GAGCAAGGGA GAACAATACC ATAATATGAT GCAGCCCTCT 
GCTGAGTATC TTGGCTCGTT ACTCAGCATA GACCAATGGG CAGTCAATAT CTTCACAGAA 
GAAATTATAC GCGGTGGATC AGCTGCTACT CTGTCTGCTC TTCTGAACCG ATTTGATCCT 
GTTTTAAGGA ATGTTGCTCA CCTCGGAAGT TGGCAGGTTA TAAGCCCGGT TGAAGTATCA 
GGTTATGTGG TTGTGGTTGA TGAGTTACTT GCTGTCCAGA ACAAATCTTA TGATAAACCA 
ACCATCCTTG TGGCAAAGAG TGTCAAGGGA GAGGAAGAAA TACCAGATGG AGTAGTTGGT 
GTAATTACAC CTGATATGCC AGATGTTCTG TCTCATGTGT CAGTCCGAGC AAGGAATAGC 
AAGGTACTGT TTGCGACCTG TTTTGACCAC ACCACTCTAT CTGAACTTGA AGGATATGAT 
CAGAAACTGT TTTCCTTCAA GCCTACTTCT GCAGATATAA CCTATAGGGA GATCACAGAG 
AGTGAACTTC AGCAATCAAG TTCTCCAAAT GCAGAAGTTG GCCATGCAGT ACCATCTATT 
TCATTGGCCA AGAAGAAATT TCTTGGAAAA TATGCAATAT CAGCCGAAGA ATTCTCTGAG 
GAAATGGTTG GGGCCAAGTC TCGGAATATA GCATACCTCA AAGGAAAAGT ACCTTCATGG 
GTCGGTGTCC CAACGTCAGT TGCGATACCA TTTGGCACTT TTGAGAAGGT TTTGTCAGAT 
GGGCTTAATA AGGAAGTAGC ACAGAGCATA GAGAAGCTTA AGATCAGACT TGCCCAAGAA 
GATTTTAGTG CTCTAGGTGA AATAAGAAAA GTCGTCCTTA ATCTTACTGC TCCTATGCAA 
TTGGTTAATG AGCTGAAGGA GAGGATGCTA GGCTCTGGAA TGCCCTGGCC TGGTGATGAA 
GGAGACAAGC GTTGGGAGCA AGCATGGATG GCTATTAAAA AGGTTTGGGC ATCAAAATGG 
AACGAAAGAG CATATTTTAG CACACGCAAG GTGAAACTTG ATCATGAGTA CCTTTCGATG 
GCTGTTCTCG TGCAAGAAGT TGTGAATGCA GATTATGCTT TTGTCATTCA TACCACAAAC 
CCATCGTCTG GAGATTCTTC TGAGATATAT GCTGAAGTGG TGAAAGGGCT TGGCGAGACC 
CTCGTGGGAG CCTATCCTGG TCGTGCTATG AGCTTTGTTT GCAAAAAAGA TGACCTTGAC 
TCTCCCAAGT TACTTGGTTA CCCAAGCAAG CCAATTGGTC TCTTCATAAG GCAATCAATC 
ATCTTCCGTT CCGACTCCAA CGGTGAGGAC CTGGAAGGTT ATGCTGGAGC AGGATTATAT 
GATAGTGTAC CGATGGATGA GGAGGATGAG GTTGTACTTG ATTATACAAC TGACCCTCTT 
ATAGTAGACC GTGGATTCCG AAGCTCAATC CTCTCAAGCA TAGCACGGGC TGGCCATGCC 
ATCGAGGAGC TATATGGTTC TCCTCAGGAC GTCGAGGGAG TAGTGAAGGA TGGAAAAATC 
TATGTAGTCC AGACAAGACC ACAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020394628.1
CDS	296..4705
Translation	MSGFSAAANAAAAERCALAFRARPAASSPAKRQQQPQPASLRRSGGQRRPTTLSASSRGPVVPRAVATSADRASPDLIGKFTLDSNSELQVAVNPAPQGLVSEISLEVTNTSGSLILHWGALRPDKRDWILPSRKPDGTTVYKNRALRTPFVKSGDNSTLRIEIDDPGVHAIEFLIFDETQNKWFKNNGQNFQVQFQSSRHQGTGASGASSSATSTLVPEDLVQIQAYLRWERRGKQSYTPEQEKEEYEAARAELIEEVNRGVSLEKLRAKLTKAPEAPESDESKSSASRMPIGKLPEDLVQVQAYIRWEQAGKPNYPPEKQLVEFEEARKELQAEVDKGISIDQLRQKILKGNIESKVSKQLKNKKYFSVERIQRKKRDITQLLSKHKHTLVEDKVEVVPKQPTVLDLFTKSLHEKDGCEVLSRKLFKFGDKEILAISTKVQNKTEVHLATNHTDPLILHWSLAKNAGEWKAPSPNILPSGSTLLDKACETEFTKSELDGLHYQVVEIELDDGGYKGMPFVLRSGETWIKNNGSDFFLDFSTHDVRNIKLKGNGDAGKGTAKALLERIADLEEDAQRSLMHRFNIAADLADQARDAGLLGIVGLFVWIRFMATRQLTWNKNYNVKPREISKAQDRFTDDLENMYKAYPQYREILRMIMAAVGRGGEGDVGQRIRDEILVIQRNNDCKGGMMEEWHQKLHNNTSPDDVVICQALIDYIKSDFDISVYWDTLNKNGITKERLLSYDRAIHSEPNFRSEQKAGLLRDLGNYMRSLKAVHSGADLESAIASCMGYKSEGEGFMVGVQINPVKGLPSGFPELLEFVLEHVEDKSAEPLLEGLLEARVELRPLLLDSRERMKDLIFLDIALDSTFRTAIERSYEELNDAAPEKIMYFISLVLENLALSIDDNEDILYCLKGWNQALEMAKQKDDQWALYAKAFLDRNRLALASKGEQYHNMMQPSAEYLGSLLSIDQWAVNIFTEEIIRGGSAATLSALLNRFDPVLRNVAHLGSWQVISPVEVSGYVVVVDELLAVQNKSYDKPTILVAKSVKGEEEIPDGVVGVITPDMPDVLSHVSVRARNSKVLFATCFDHTTLSELEGYDQKLFSFKPTSADITYREITESELQQSSSPNAEVGHAVPSISLAKKKFLGKYAISAEEFSEEMVGAKSRNIAYLKGKVPSWVGVPTSVAIPFGTFEKVLSDGLNKEVAQSIEKLKIRLAQEDFSALGEIRKVVLNLTAPMQLVNELKERMLGSGMPWPGDEGDKRWEQAWMAIKKVWASKWNERAYFSTRKVKLDHEYLSMAVLVQEVVNADYAFVIHTTNPSSGDSSEIYAEVVKGLGETLVGAYPGRAMSFVCKKDDLDSPKLLGYPSKPIGLFIRQSIIFRSDSNGEDLEGYAGAGLYDSVPMDEEDEVVLDYTTDPLIVDRGFRSSILSSIARAGHAIEELYGSPQDVEGVVKDGKIYVVQTRPQM

Target ORF information:

RefSeq Version	XM_020539039.2
Organism	Zea mays
Definition	Zea mays Alpha-glucan water dikinase 1 chloroplastic (AY109804), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020539039.2

ORF Insert Sequence:

ATGTCCGGAT TCAGTGCCGC GGCCAACGCA GCGGCGGCTG AGCGGTGCGC GCTCGCGTTC 
CGCGCACGGC CCGCGGCCTC CTCGCCAGCG AAGCGGCAGC AGCAGCCGCA GCCAGCGTCC 
CTCCGACGCA GCGGGGGCCA GCGCCGCCCC ACGACGCTCT CCGCCTCTAG CCGCGGCCCC 
GTCGTGCCGC GCGCCGTCGC CACGTCCGCG GACCGCGCGT CCCCCGACCT TATCGGAAAG 
TTCACGCTGG ATTCCAACTC CGAGCTCCAG GTCGCAGTGA ACCCAGCGCC GCAGGGTTTG 
GTGTCAGAGA TTAGCCTGGA GGTGACCAAC ACAAGCGGTT CCCTGATTTT GCATTGGGGA 
GCCCTTCGCC CGGACAAGAG AGATTGGATC CTCCCGTCCA GAAAACCTGA TGGAACGACA 
GTGTACAAGA ACAGGGCTCT CAGGACACCT TTTGTAAAGT CAGGTGATAA CTCCACTCTA 
AGGATTGAGA TAGATGATCC TGGGGTGCAC GCCATTGAGT TCCTCATCTT TGACGAGACA 
CAGAACAAAT GGTTTAAAAA CAATGGCCAG AATTTTCAGG TTCAGTTCCA GTCGAGCCGC 
CATCAGGGTA CTGGTGCATC TGGTGCCTCC TCTTCTGCTA CTTCTACCTT GGTGCCAGAG 
GATCTTGTGC AGATCCAAGC TTACCTTCGG TGGGAAAGAA GGGGAAAGCA GTCATACACA 
CCAGAGCAAG AAAAGGAGGA GTATGAAGCT GCACGAGCTG AGTTAATAGA GGAAGTAAAC 
AGAGGTGTTT CTTTAGAGAA GCTTCGAGCT AAATTGACAA AAGCACCTGA AGCACCTGAG 
TCGGATGAAA GTAAATCTTC TGCATCTCGA ATGCCCATCG GTAAACTTCC AGAGGATCTT 
GTACAGGTGC AGGCTTATAT AAGGTGGGAG CAAGCGGGCA AGCCAAACTA TCCTCCTGAG 
AAGCAACTGG TAGAATTTGA GGAAGCAAGG AAGGAACTGC AGGCTGAGGT GGACAAGGGA 
ATCTCTATTG ATCAGTTGAG GCAGAAAATT TTGAAAGGAA ACATTGAGAG TAAAGTTTCC 
AAGCAGCTGA AGAACAAGAA GTACTTCTCT GTAGAAAGGA TTCAGCGCAA AAAGAGAGAT 
ATCACACAAC TTCTCAGTAA ACATAAGCAT ACACTTGTGG AAGATAAAGT AGAGGTTGTA 
CCAAAACAAC CAACTGTTCT TGATCTCTTC ACCAAGTCTT TACATGAGAA GGATGGCTGT 
GAAGTTCTAA GCAGAAAGCT CTTCAAGTTC GGCGATAAAG AGATACTGGC AATTTCTACC 
AAGGTTCAAA ATAAAACAGA AGTTCACTTG GCAACAAACC ATACCGACCC ACTTATTCTT 
CACTGGTCTT TGGCAAAAAA TGCTGGAGAA TGGAAGGCAC CTTCTCCAAA TATATTGCCA 
TCTGGTTCCA CATTGCTGGA CAAGGCGTGT GAAACTGAAT TTACTAAATC TGAATTGGAT 
GGTTTGCATT ACCAGGTTGT TGAGATAGAG CTTGATGATG GAGGATACAA AGGAATGCCA 
TTTGTTCTTC GGTCTGGTGA AACATGGATA AAAAATAATG GTTCTGATTT TTTCCTAGAT 
TTCAGCACCC ATGATGTCAG AAATATTAAG TTAAAGGGCA ATGGTGATGC TGGTAAAGGT 
ACTGCTAAGG CATTGCTGGA GAGAATAGCA GATCTGGAGG AAGATGCCCA GCGATCTCTT 
ATGCACAGAT TCAATATTGC AGCAGATCTA GCTGACCAAG CCAGAGATGC TGGACTTTTG 
GGTATTGTTG GGCTTTTTGT TTGGATTAGA TTCATGGCTA CCAGGCAACT AACATGGAAT 
AAGAACTATA ATGTGAAGCC ACGTGAGATA AGCAAAGCAC AGGATAGGTT TACAGATGAT 
CTTGAGAATA TGTACAAAGC TTATCCACAG TACAGAGAGA TATTAAGAAT GATAATGGCT 
GCTGTTGGTC GCGGAGGTGA AGGTGATGTT GGTCAACGCA TTCGTGATGA GATATTAGTA 
ATACAGAGAA ATAATGACTG CAAAGGTGGA ATGATGGAAG AATGGCACCA GAAATTGCAC 
AACAATACAA GCCCAGATGA TGTAGTGATA TGCCAGGCCT TAATTGATTA TATCAAGAGT 
GACTTTGATA TAAGCGTTTA CTGGGACACC TTGAACAAAA ATGGCATAAC CAAAGAGCGT 
CTCTTGAGCT ATGATCGTGC TATTCATTCA GAACCAAATT TCAGAAGTGA ACAGAAGGCG 
GGTTTACTCC GTGACCTGGG AAATTACATG AGAAGCCTAA AGGCTGTGCA TTCTGGTGCT 
GATCTTGAAT CTGCTATAGC AAGTTGTATG GGATACAAAT CAGAGGGTGA AGGTTTCATG 
GTTGGTGTTC AGATCAATCC AGTGAAGGGT TTACCATCTG GATTTCCGGA GTTGCTTGAA 
TTTGTGCTTG AACATGTTGA GGATAAATCA GCGGAACCAC TTCTTGAGGG GCTATTGGAA 
GCTCGAGTTG AACTGCGCCC TTTGCTTCTT GATTCGCGTG AACGCATGAA AGATCTTATA 
TTTTTGGACA TTGCTCTTGA TTCTACCTTC AGGACAGCAA TTGAAAGGTC ATATGAGGAG 
CTGAATGATG CAGCCCCAGA GAAAATAATG TACTTCATCA GTCTTGTCCT TGAAAATCTT 
GCGCTTTCAA TTGACGACAA TGAAGACATC CTGTATTGTT TAAAGGGATG GAACCAAGCC 
TTGGAAATGG CTAAGCAAAA AGACGACCAA TGGGCGCTCT ATGCTAAAGC ATTTCTTGAC 
AGAAACAGAC TTGCCCTTGC GAGCAAGGGA GAACAATACC ATAATATGAT GCAGCCCTCT 
GCTGAGTATC TTGGCTCGTT ACTCAGCATA GACCAATGGG CAGTCAATAT CTTCACAGAA 
GAAATTATAC GCGGTGGATC AGCTGCTACT CTGTCTGCTC TTCTGAACCG ATTTGATCCT 
GTTTTAAGGA ATGTTGCTCA CCTCGGAAGT TGGCAGGTTA TAAGCCCGGT TGAAGTATCA 
GGTTATGTGG TTGTGGTTGA TGAGTTACTT GCTGTCCAGA ACAAATCTTA TGATAAACCA 
ACCATCCTTG TGGCAAAGAG TGTCAAGGGA GAGGAAGAAA TACCAGATGG AGTAGTTGGT 
GTAATTACAC CTGATATGCC AGATGTTCTG TCTCATGTGT CAGTCCGAGC AAGGAATAGC 
AAGGTACTGT TTGCGACCTG TTTTGACCAC ACCACTCTAT CTGAACTTGA AGGATATGAT 
CAGAAACTGT TTTCCTTCAA GCCTACTTCT GCAGATATAA CCTATAGGGA GATCACAGAG 
AGTGAACTTC AGCAATCAAG TTCTCCAAAT GCAGAAGTTG GCCATGCAGT ACCATCTATT 
TCATTGGCCA AGAAGAAATT TCTTGGAAAA TATGCAATAT CAGCCGAAGA ATTCTCTGAG 
GAAATGGTTG GGGCCAAGTC TCGGAATATA GCATACCTCA AAGGAAAAGT ACCTTCATGG 
GTCGGTGTCC CAACGTCAGT TGCGATACCA TTTGGCACTT TTGAGAAGGT TTTGTCAGAT 
GGGCTTAATA AGGAAGTAGC ACAGAGCATA GAGAAGCTTA AGATCAGACT TGCCCAAGAA 
GATTTTAGTG CTCTAGGTGA AATAAGAAAA GTCGTCCTTA ATCTTACTGC TCCTATGCAA 
TTGGTTAATG AGCTGAAGGA GAGGATGCTA GGCTCTGGAA TGCCCTGGCC TGGTGATGAA 
GGAGACAAGC GTTGGGAGCA AGCATGGATG GCTATTAAAA AGGTTTGGGC ATCAAAATGG 
AACGAAAGAG CATATTTTAG CACACGCAAG GTGAAACTTG ATCATGAGTA CCTTTCGATG 
GCTGTTCTCG TGCAAGAAGT TGTGAATGCA GATTATGCTT TTGTCATTCA TACCACAAAC 
CCATCGTCTG GAGATTCTTC TGAGATATAT GCTGAAGTGG TGAAAGGGCT TGGCGAGACC 
CTCGTGGGAG CCTATCCTGG TCGTGCTATG AGCTTTGTTT GCAAAAAAGA TGACCTTGAC 
TCTCCCAAGT TACTTGGTTA CCCAAGCAAG CCAATTGGTC TCTTCATAAG GCAATCAATC 
ATCTTCCGTT CCGACTCCAA CGGTGAGGAC CTGGAAGGTT ATGCTGGAGC AGGATTATAT 
GATAGTGTAC CGATGGATGA GGAGGATGAG GTTGTACTTG ATTATACAAC TGACCCTCTT 
ATAGTAGACC GTGGATTCCG AAGCTCAATC CTCTCAAGCA TAGCACGGGC TGGCCATGCC 
ATCGAGGAGC TATATGGTTC TCCTCAGGAC GTCGAGGGAG TAGTGAAGGA TGGAAAAATC 
TATGTAGTCC AGACAAGACC ACAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.
PLoS genetics5(11)e1000740(2009 Nov)
Soderlund C,Descour A,Kudrna D,Bomhoff M,Boyd L,Currie J,Angelova A,Collura K,Wissotski M,Ashley E,Morrow D,Fernandes J,Walbot V,Yu Y

Anchoring 9,371 maize expressed sequence tagged unigenes to the bacterial artificial chromosome contig map by two-dimensional overgo hybridization.
Plant physiology134(4)1317-26(2004 Apr)
Gardiner J,Schroeder S,Polacco ML,Sanchez-Villeda H,Fang Z,Morgante M,Landewe T,Fengler K,Useche F,Hanafey M,Tingey S,Chou H,Wing R,Soderlund C,Coe EH Jr

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AY109804 cDNA ORF clone, Zea mays

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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