LOC100281040 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100281040
Entrez Gene ID	100281040
Full Name	aconitase 2
Synonyms	GRMZM5G858454,aconitase2
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
XM_008663662.2	XP_008661884.2	aconitase 2 isoform X1
XM_020545035.1	XP_020400624.1	aconitase 2 isoform X3
NM_001153959.2	NP_001147431.1	aconitase 2 isoform 1
XM_020545035.2	XP_020400624.1	aconitase 2 isoform X2
XM_020545034.1	XP_020400623.1	aconitase 2 isoform X2
XM_020545034.2	XP_020400623.1	aconitase 2 isoform X1
NM_001358373.1	NP_001345302.1	aconitase 2 isoform 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The B73 maize genome: complexity, diversity, and dynamics.
Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, Liang C, Zhang J, Fulton L, Graves TA, Minx P, Reily AD, Courtney L, Kruchowski SS, Tomlinson C, Strong C, Delehaunty K, Fronick C, Courtney B, Rock SM, Belter E, Du F, Kim K, Abbott RM, Cotton M, Levy A, Marchetto P, Ochoa K, Jackson SM, Gillam B, Chen W, Yan L, Higginbotham J, Cardenas M, Waligorski J, Applebaum E, Phelps L, Falcone J, Kanchi K, Thane T, Scimone A, Thane N, Henke J, Wang T, Ruppert J, Shah N, Rotter K, Hodges J, Ingenthron E, Cordes M, Kohlberg S, Sgro J, Delgado B, Mead K, Chinwalla A, Leonard S, Crouse K, Collura K, Kudrna D, Currie J, He R, Angelova A, Rajasekar S, Mueller T, Lomeli R, Scara G, Ko A, Delaney K, Wissotski M, Lopez G, Campos D, Braidotti M, Ashley E, Golser W, Kim H, Lee S, Lin J, Dujmic Z, Kim W, Talag J, Zuccolo A, Fan C, Sebastian A, Kramer M, Spiegel L, Nascimento L, Zutavern T, Miller B, Ambroise C, Muller S, Spooner W, Narechania A, Ren L, Wei S, Kumari S, Faga B, Levy MJ, McMahan L, Van Buren P, Vaughn MW, Ying K, Yeh CT, Emrich SJ, Jia Y, Kalyanaraman A, Hsia AP, Barbazuk WB, Baucom RS, Brutnell TP, Carpita NC, Chaparro C, Chia JM, Deragon JM, Estill JC, Fu Y, Jeddeloh JA, Han Y, Lee H, Li P, Lisch DR, Liu S, Liu Z, Nagel DH, McCann MC, SanMiguel P, Myers AM, Nettleton D, Nguyen J, Penning BW, Ponnala L, Schneider KL, Schwartz DC, Sharma A, Soderlund C, Springer NM, Sun Q, Wang H, Waterman M, Westerman R, Wolfgruber TK, Yang L, Yu Y, Zhang L, Zhou S, Zhu Q, Bennetzen JL, Dawe RK, Jiang J, Jiang N, Presting GG, Wessler SR, Aluru S, Martienssen RA, Clifton SW, McCombie WR, Wing RA, Wilson RK
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC100281040 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100281040 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OZa02471	NM_001153959.2 Latest version!	Zea mays aconitase 2 (LOC100281040), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OZa56261	NM_001358373.1 Latest version!	Zea mays aconitase 2 (LOC100281040), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OZa56261	XM_008663662.2 Latest version!	Zea mays aconitase 2 (aco2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OZa56262	XM_020545034.2 Latest version!	Zea mays aconitase 2 (aco2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OZa56263	XM_020545035.2 Latest version!	Zea mays aconitase 2 (aco2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OZa56263	XM_020545035.1	Zea mays aconitase 2 (aco2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OZa56262	XM_020545034.1	Zea mays aconitase 2 (aco2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100281040 ( NM_001153959.2 ) cDNA ORF clone, Zea mays -> NP_001147431.1 Zea mays aconitase 2 (LOC100281040), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OZa02471
Clone ID Related Accession (Same CDS sequence)	NM_001153959.2
Accession Version	NM_001153959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556467200000
Organism	Zea mays
Product	aconitase 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EU955377.1. On Aug 15, 2014 this sequence version replaced NM_001153959.1. Transcript Variant: This variant (1)has the nineteenth exon. ##Evidence-Data-START## Transcript exon combination :: EU955377.1, SRR3147048.1343.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01096209 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCCAACC CAACAGCTAC CAAGCATGCT TTCAAGCGTA TTTTGACGAG CCTGCTTAAG 
CCTGGCGGTG GTGAATACGG CAAGTTCTTC AGCCTTCCGG CGCTAAATGA TCCAAGGATC 
GATAAGCTTC CCTACTCTAT ACGTGTTCTC CTGGAATCAG CTATCCGCCA TTGTGATAAC 
TTCCAAGTTA CAGAGAGTGA TGTTGAGAAA ATCATTGATT GGGAGAACAC ATCTCCAAAG 
CTGGCTGAGA TACCTTTCAA GCCAGCGAGA TGCATTCTAA TGGACAACAC TGGAGTCCCA 
GCAGTTGTAG ACCTTGCAGC TATGCGTGAT ATGATGCCAA AGTTGGGATG TGATCCCTAC 
AAGATTAATC CATTGATTCC CGTGGATGCT GTTATTGACC ACGCAGTGCG AGTGGATGTA 
GCAGGGACCT ATGATGCATT GGATAGAAAC GAGGAGCTGG AGTTCCAACG CAACAAAGAA 
AGGTTTGCTT TTCTTAAATG GGCATCCAAT GCTTTCCACA ACATGCAGGT TTTCCCTCCT 
GGTTCGGGCA CTGTACACCA GGTAAATCTT GAGTATCTTG CCCGAGTTGT CTTCAACGAA 
GATGGCATTC TTTACTTCGA CAGCGTGGTT GGCACAGACT CACACACCAC TATGATTAAT 
AGTCTTGGAG TTGCTGGGTG GGGAGTAGGC GGTATTGAAG CAGTCGTTGC AATGCTTGGC 
CAGCCAATGG GCATGGTTTT GCCTGGTGTG GTTGGATTCA AATTGAGTGG GAAGTTACGG 
GATGGTGTCA CTACTACTGA CATTGTTCTT ACCATGACCC AAATGCTAAG GAAGCACGGT 
GCTATTGGCA AATTCGTTGA ATTCTATGGT GTTGGTGTGG GCGAGCTATC TTTGCCTGCT 
AGGGCCACAA TTGCCAACAT GTCTCCAGAA TATGGAGCTA CCATGGGCTT CTTCCCTGTA 
GACCAAGTAG CATTGGACTA TCTCAAACTG ACAGGCCGAA GCGATGAAAC TGTGTCGATG 
ATTGAAGCAT ACCTGCGAGC TAACAAAATG TTTGTGGACA AGCATGAGCC TGAGACGGAA 
CGCGTTTTCT CTTCACATCT GGAGCTGGAC CTTAGTGAGG TGGAGCCTTG CGTTTCAGGC 
CCCAAAAGGC CTCATGATCG TGTCCCTTTG AAGGAAATGA AGTCAGACTG GCATGCTTGC 
CTGGACAACG AAGTTGGCTT CAAGGGCTAT GCGGTGCCAA AGGAACAGCA AGGCAAAGTT 
GTGAAATTCG ACTTCCATGG AAGGCCAGCT GAAATCAAGC ATGGCAGTGT TGTTCTTGCA 
GCAATATGTA GCTCCACAAA CACATCAAAT CCCAGCGTCA TGATTGGTGC TGGCCTTGTG 
GCAAAGAAAG CCTGCGAATT AGGCCTTGAG GTGAAGCCAT GGGTAAAGAC AAGCCTTACC 
CCTGGATCTG TGGTTGCTAC CGAGTACTTG AAACATAGTG GCCTTCAAGA TTATCTGAAC 
CAGCAAGGGT TCCATGTCGC TGCGCATGGT TGCGCCACTT GTGTGGGCAA CTCCGGTGAC 
CTGGATGGAT CTGTATCAGC TGCTATCACA GAAAATGATA TGGTTGCTGC TGCGGTGCTG 
TCGGCCAACC GAAACTTCGA GGGGCGTGTG AACCCTCTCA CTCGGGCTAA CTACCTTGCC 
TCGCCGCCTC TGGTTGTTGC CTATGCTCTT GCCGGCACTG TTGACATTGA CTTTGAGAAA 
GAGCCCATTG GAGTTGGAAA GGGTGGTAAG GAAGTTTTCT TGAGGGACAT ATGGCCTTCA 
AACCAAGAGA TCGATGAGGT TGTGGAGTCC AGCGTGCAGA CTCACCTGTT CAAGAAAGTG 
TACGACTCAA TCATGGAACG TAACCCTAGG TGGAACCAGC TGCCGGTTCC AAAGGAGGCA 
CTGTACCCAT GGGAAGACAG GTCCACCTAC ATCCGCAAGC CTACGTACCT GGAGGGCATG 
TCCATGACGC CGCCTGCAGC CCCGCCCACG GTGACGGAGG CCTACTGCCT GCTCAACCTT 
GGGGACAGCA TCACCACGGA CCACATCTCC TACTCGGGGA AGATCCCCGA GGGCACACCT 
GCGGCCAAGT ATCTGCTCGA GTATGGCGTG GAGCCCAAGA ACTTCAGCTC CTATGGTGGC 
CGCCGCGGGA ACAACGAGGT GGTGATGAGG GGAGCGTTTG CCAATATGAG GATCGTGAAT 
AAGCTTCTTG ATGGCAAGGC TGGTCCCTGG ACGATCCATG TTCCTACCGG GGAGAAGCTC 
TATGTTTACG ACGCGGCAAT GAAGTACAAG TCGGAAGGTC ATGACATGGT CATCATTGCT 
GGCTCCGAGT ACGGCAGTGG CAGCTCTCGT GACTCTGCTG CCAAGGGACC AATGCTACTG 
GGTGTCAAGT CGGTGATTGC AAAGAGCTTC GAGCGGATCC ACCGGAGCAA CTTGGTGGGG 
ATGGGGATCA TTCCTCTCTG CTTTAAAGCA GGAGAGGATG CCGATTCACT CGGCCTCACC 
GGACGTGAGC GCTACACCAT CCACCTCCCT ACCAGCACCG CCGAGCTCAG TCCAGGCCAG 
GACGTGACCG TCACAACCCA CGACGGCAGA TCCTTCACTT GCACTCTTCG CTTGGACACA 
CAGCTTGAGG TCACATACTT CAACCATGGA GGCATCCTTC CCTACATGGT CCGCAACCTG 
GCAGCACAGA TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001147431.1
CDS	89..2806
Translation	MANPTATKHAFKRILTSLLKPGGGEYGKFFSLPALNDPRIDKLPYSIRVLLESAIRHCDNFQVTESDVEKIIDWENTSPKLAEIPFKPARCILMDNTGVPAVVDLAAMRDMMPKLGCDPYKINPLIPVDAVIDHAVRVDVAGTYDALDRNEELEFQRNKERFAFLKWASNAFHNMQVFPPGSGTVHQVNLEYLARVVFNEDGILYFDSVVGTDSHTTMINSLGVAGWGVGGIEAVVAMLGQPMGMVLPGVVGFKLSGKLRDGVTTTDIVLTMTQMLRKHGAIGKFVEFYGVGVGELSLPARATIANMSPEYGATMGFFPVDQVALDYLKLTGRSDETVSMIEAYLRANKMFVDKHEPETERVFSSHLELDLSEVEPCVSGPKRPHDRVPLKEMKSDWHACLDNEVGFKGYAVPKEQQGKVVKFDFHGRPAEIKHGSVVLAAICSSTNTSNPSVMIGAGLVAKKACELGLEVKPWVKTSLTPGSVVATEYLKHSGLQDYLNQQGFHVAAHGCATCVGNSGDLDGSVSAAITENDMVAAAVLSANRNFEGRVNPLTRANYLASPPLVVAYALAGTVDIDFEKEPIGVGKGGKEVFLRDIWPSNQEIDEVVESSVQTHLFKKVYDSIMERNPRWNQLPVPKEALYPWEDRSTYIRKPTYLEGMSMTPPAAPPTVTEAYCLLNLGDSITTDHISYSGKIPEGTPAAKYLLEYGVEPKNFSSYGGRRGNNEVVMRGAFANMRIVNKLLDGKAGPWTIHVPTGEKLYVYDAAMKYKSEGHDMVIIAGSEYGSGSSRDSAAKGPMLLGVKSVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGRERYTIHLPTSTAELSPGQDVTVTTHDGRSFTCTLRLDTQLEVTYFNHGGILPYMVRNLAAQMK

Target ORF information:

RefSeq Version	NM_001153959.2
Organism	Zea mays
Definition	Zea mays aconitase 2 (LOC100281040), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001153959.2

ORF Insert Sequence:

ATGGCCAACC CAACAGCTAC CAAGCATGCT TTCAAGCGTA TTTTGACGAG CCTGCTTAAG 
CCTGGCGGTG GTGAATACGG CAAGTTCTTC AGCCTTCCGG CGCTAAATGA TCCAAGGATC 
GATAAGCTTC CCTACTCTAT ACGTGTTCTC CTGGAATCAG CTATCCGCCA TTGTGATAAC 
TTCCAAGTTA CAGAGAGTGA TGTTGAGAAA ATCATTGATT GGGAGAACAC ATCTCCAAAG 
CTGGCTGAGA TACCTTTCAA GCCAGCGAGA TGCATTCTAA TGGACAACAC TGGAGTCCCA 
GCAGTTGTAG ACCTTGCAGC TATGCGTGAT ATGATGCCAA AGTTGGGATG TGATCCCTAC 
AAGATTAATC CATTGATTCC CGTGGATGCT GTTATTGACC ACGCAGTGCG AGTGGATGTA 
GCAGGGACCT ATGATGCATT GGATAGAAAC GAGGAGCTGG AGTTCCAACG CAACAAAGAA 
AGGTTTGCTT TTCTTAAATG GGCATCCAAT GCTTTCCACA ACATGCAGGT TTTCCCTCCT 
GGTTCGGGCA CTGTACACCA GGTAAATCTT GAGTATCTTG CCCGAGTTGT CTTCAACGAA 
GATGGCATTC TTTACTTCGA CAGCGTGGTT GGCACAGACT CACACACCAC TATGATTAAT 
AGTCTTGGAG TTGCTGGGTG GGGAGTAGGC GGTATTGAAG CAGTCGTTGC AATGCTTGGC 
CAGCCAATGG GCATGGTTTT GCCTGGTGTG GTTGGATTCA AATTGAGTGG GAAGTTACGG 
GATGGTGTCA CTACTACTGA CATTGTTCTT ACCATGACCC AAATGCTAAG GAAGCACGGT 
GCTATTGGCA AATTCGTTGA ATTCTATGGT GTTGGTGTGG GCGAGCTATC TTTGCCTGCT 
AGGGCCACAA TTGCCAACAT GTCTCCAGAA TATGGAGCTA CCATGGGCTT CTTCCCTGTA 
GACCAAGTAG CATTGGACTA TCTCAAACTG ACAGGCCGAA GCGATGAAAC TGTGTCGATG 
ATTGAAGCAT ACCTGCGAGC TAACAAAATG TTTGTGGACA AGCATGAGCC TGAGACGGAA 
CGCGTTTTCT CTTCACATCT GGAGCTGGAC CTTAGTGAGG TGGAGCCTTG CGTTTCAGGC 
CCCAAAAGGC CTCATGATCG TGTCCCTTTG AAGGAAATGA AGTCAGACTG GCATGCTTGC 
CTGGACAACG AAGTTGGCTT CAAGGGCTAT GCGGTGCCAA AGGAACAGCA AGGCAAAGTT 
GTGAAATTCG ACTTCCATGG AAGGCCAGCT GAAATCAAGC ATGGCAGTGT TGTTCTTGCA 
GCAATATGTA GCTCCACAAA CACATCAAAT CCCAGCGTCA TGATTGGTGC TGGCCTTGTG 
GCAAAGAAAG CCTGCGAATT AGGCCTTGAG GTGAAGCCAT GGGTAAAGAC AAGCCTTACC 
CCTGGATCTG TGGTTGCTAC CGAGTACTTG AAACATAGTG GCCTTCAAGA TTATCTGAAC 
CAGCAAGGGT TCCATGTCGC TGCGCATGGT TGCGCCACTT GTGTGGGCAA CTCCGGTGAC 
CTGGATGGAT CTGTATCAGC TGCTATCACA GAAAATGATA TGGTTGCTGC TGCGGTGCTG 
TCGGCCAACC GAAACTTCGA GGGGCGTGTG AACCCTCTCA CTCGGGCTAA CTACCTTGCC 
TCGCCGCCTC TGGTTGTTGC CTATGCTCTT GCCGGCACTG TTGACATTGA CTTTGAGAAA 
GAGCCCATTG GAGTTGGAAA GGGTGGTAAG GAAGTTTTCT TGAGGGACAT ATGGCCTTCA 
AACCAAGAGA TCGATGAGGT TGTGGAGTCC AGCGTGCAGA CTCACCTGTT CAAGAAAGTG 
TACGACTCAA TCATGGAACG TAACCCTAGG TGGAACCAGC TGCCGGTTCC AAAGGAGGCA 
CTGTACCCAT GGGAAGACAG GTCCACCTAC ATCCGCAAGC CTACGTACCT GGAGGGCATG 
TCCATGACGC CGCCTGCAGC CCCGCCCACG GTGACGGAGG CCTACTGCCT GCTCAACCTT 
GGGGACAGCA TCACCACGGA CCACATCTCC TACTCGGGGA AGATCCCCGA GGGCACACCT 
GCGGCCAAGT ATCTGCTCGA GTATGGCGTG GAGCCCAAGA ACTTCAGCTC CTATGGTGGC 
CGCCGCGGGA ACAACGAGGT GGTGATGAGG GGAGCGTTTG CCAATATGAG GATCGTGAAT 
AAGCTTCTTG ATGGCAAGGC TGGTCCCTGG ACGATCCATG TTCCTACCGG GGAGAAGCTC 
TATGTTTACG ACGCGGCAAT GAAGTACAAG TCGGAAGGTC ATGACATGGT CATCATTGCT 
GGCTCCGAGT ACGGCAGTGG CAGCTCTCGT GACTCTGCTG CCAAGGGACC AATGCTACTG 
GGTGTCAAGT CGGTGATTGC AAAGAGCTTC GAGCGGATCC ACCGGAGCAA CTTGGTGGGG 
ATGGGGATCA TTCCTCTCTG CTTTAAAGCA GGAGAGGATG CCGATTCACT CGGCCTCACC 
GGACGTGAGC GCTACACCAT CCACCTCCCT ACCAGCACCG CCGAGCTCAG TCCAGGCCAG 
GACGTGACCG TCACAACCCA CGACGGCAGA TCCTTCACTT GCACTCTTCG CTTGGACACA 
CAGCTTGAGG TCACATACTT CAACCATGGA GGCATCCTTC CCTACATGGT CCGCAACCTG 
GCAGCACAGA TGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100281040 ( NM_001358373.1 ) cDNA ORF clone, Zea mays -> NP_001345302.1 Zea mays aconitase 2 (LOC100281040), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OZa56261
Clone ID Related Accession (Same CDS sequence)	XM_008663662.2 , NM_001358373.1
Accession Version	NM_001358373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556467200000
Organism	Zea mays
Product	aconitase 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BT063513.1 and DR814260.1. On Dec 1, 2017 this sequence version replaced XM_008663662.2. Transcript Variant: This variant (2) lacks the nineteenth exon. ##Evidence-Data-START## Transcript exon combination :: SRR3147048.2547.1, SRR3147054.2630.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01096209 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END##

ATGGCCAACC CAACAGCTAC CAAGCATGCT TTCAAGCGTA TTTTGACGAG CCTGCTTAAG 
CCTGGCGGTG GTGAATACGG CAAGTTCTTC AGCCTTCCGG CGCTAAATGA TCCAAGGATC 
GATAAGCTTC CCTACTCTAT ACGTGTTCTC CTGGAATCAG CTATCCGCCA TTGTGATAAC 
TTCCAAGTTA CAGAGAGTGA TGTTGAGAAA ATCATTGATT GGGAGAACAC ATCTCCAAAG 
CTGGCTGAGA TACCTTTCAA GCCAGCGAGA TGCATTCTAA TGGACAACAC TGGAGTCCCA 
GCAGTTGTAG ACCTTGCAGC TATGCGTGAT ATGATGCCAA AGTTGGGATG TGATCCCTAC 
AAGATTAATC CATTGATTCC CGTGGATGCT GTTATTGACC ACGCAGTGCG AGTGGATGTA 
GCAGGGACCT ATGATGCATT GGATAGAAAC GAGGAGCTGG AGTTCCAACG CAACAAAGAA 
AGGTTTGCTT TTCTTAAATG GGCATCCAAT GCTTTCCACA ACATGCAGGT TTTCCCTCCT 
GGTTCGGGCA CTGTACACCA GGTAAATCTT GAGTATCTTG CCCGAGTTGT CTTCAACGAA 
GATGGCATTC TTTACTTCGA CAGCGTGGTT GGCACAGACT CACACACCAC TATGATTAAT 
AGTCTTGGAG TTGCTGGGTG GGGAGTAGGC GGTATTGAAG CAGTCGTTGC AATGCTTGGC 
CAGCCAATGG GCATGGTTTT GCCTGGTGTG GTTGGATTCA AATTGAGTGG GAAGTTACGG 
GATGGTGTCA CTACTACTGA CATTGTTCTT ACCATGACCC AAATGCTAAG GAAGCACGGT 
GCTATTGGCA AATTCGTTGA ATTCTATGGT GTTGGTGTGG GCGAGCTATC TTTGCCTGCT 
AGGGCCACAA TTGCCAACAT GTCTCCAGAA TATGGAGCTA CCATGGGCTT CTTCCCTGTA 
GACCAAGTAG CATTGGACTA TCTCAAACTG ACAGGCCGAA GCGATGAAAC TGTGTCGATG 
ATTGAAGCAT ACCTGCGAGC TAACAAAATG TTTGTGGACA AGCATGAGCC TGAGACGGAA 
CGCGTTTTCT CTTCACATCT GGAGCTGGAC CTTAGTGAGG TGGAGCCTTG CGTTTCAGGC 
CCCAAAAGGC CTCATGATCG TGTCCCTTTG AAGGAAATGA AGTCAGACTG GCATGCTTGC 
CTGGACAACG AAGTTGGCTT CAAGGGCTAT GCGGTGCCAA AGGAACAGCA AGGCAAAGTT 
GTGAAATTCG ACTTCCATGG AAGGCCAGCT GAAATCAAGC ATGGCAGTGT TGTTCTTGCA 
GCAATATGTA GCTCCACAAA CACATCAAAT CCCAGCGTCA TGATTGGTGC TGGCCTTGTG 
GCAAAGAAAG CCTGCGAATT AGGCCTTGAG GTGAAGCCAT GGGTAAAGAC AAGCCTTACC 
CCTGGATCTG TGGTTGCTAC CGAGTACTTG AAACATAGTG GCCTTCAAGA TTATCTGAAC 
CAGCAAGGGT TCCATGTCGC TGCGCATGGT TGCGCCACTT GTGTGGGCAA CTCCGGTGAC 
CTGGATGGAT CTGTATCAGC TGCTATCACA GAAAATGATA TGGTTGCTGC TGCGGTGCTG 
TCGGCCAACC GAAACTTCGA GGGGCGTGTG AACCCTCTCA CTCGGGCTAA CTACCTTGCC 
TCGCCGCCTC TGGTTGTTGC CTATGCTCTT GCCGGCACTG TTGACATTGA CTTTGAGAAA 
GAGCCCATTG GAGTTGGAAA GGGTGGTAAG GAAGTTTTCT TGAGGGACAT ATGGCCTTCA 
AACCAAGAGA TCGATGAGGT TGTGGAGTCC AGCGTGCAGA CTCACCTGTT CAAGAAAGTG 
TACGACTCAA TCATGGAACG TAACCCTAGG TGGAACCAGC TGCCGGTTCC AAAGGAGGCA 
CTGTACCCAT GGGAAGACAG GTCCACCTAC ATCCGCAAGC CTACGTACCT GGAGGGCATG 
TCCATGACGC CGCCTGCAGC CCCGCCCACG GTGACGGAGG CCTACTGCCT GCTCAACCTT 
GGGGACAGCA TCACCACGGA CCACATCTCC TACTCGGGGA AGATCCCCGA GGGCACACCT 
GCGGCCAAGT ATCTGCTCGA GTATGGCGTG GAGCCCAAGA ACTTCAGCTC CTATGGTGGC 
CGCCGCGGGA ACAACGAGGT GGTGATGAGG GGAGCGTTTG CCAATATGAG GATCGTGAAT 
AAGCTTCTTG ATGGCAAGGC TGGTCCCTGG ACGATCCATG TTCCTACCGG GGAGAAGCTC 
TATGTTTACG ACGCGGCAAT GAAGTACAAG TCGGAAGGTC ATGACATGGT CATCATTGCT 
GGCTCCGAGT ACGGCAGTGG CAGCTCTCGT GACTCTGCTG CCAAGGGACC AATGCTACTG 
GGTGTCAAGT CGGTGATTGC AAAGAGCTTC GAGCGGATCC ACCGGAGCAA CTTGGTGGGG 
ATGGGGATCA TTCCTCTCTG CTTTAAAGCA GGAGAGGATG CCGATTCACT CGGCCTCACC 
GGACGTGAGC GCTACACCAT CCACCTCCCT ACCAGCACCG CCGAGCTCAG TCCAGGCCAG 
GACGTGACCG TCACAACCCA CGACGGCAGA TCCTTCACTT GCACTCTTCG CTTGGACACA 
CAGGTACTCA GTGCCCCACG TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345302.1
CDS	51..2717
Translation	MANPTATKHAFKRILTSLLKPGGGEYGKFFSLPALNDPRIDKLPYSIRVLLESAIRHCDNFQVTESDVEKIIDWENTSPKLAEIPFKPARCILMDNTGVPAVVDLAAMRDMMPKLGCDPYKINPLIPVDAVIDHAVRVDVAGTYDALDRNEELEFQRNKERFAFLKWASNAFHNMQVFPPGSGTVHQVNLEYLARVVFNEDGILYFDSVVGTDSHTTMINSLGVAGWGVGGIEAVVAMLGQPMGMVLPGVVGFKLSGKLRDGVTTTDIVLTMTQMLRKHGAIGKFVEFYGVGVGELSLPARATIANMSPEYGATMGFFPVDQVALDYLKLTGRSDETVSMIEAYLRANKMFVDKHEPETERVFSSHLELDLSEVEPCVSGPKRPHDRVPLKEMKSDWHACLDNEVGFKGYAVPKEQQGKVVKFDFHGRPAEIKHGSVVLAAICSSTNTSNPSVMIGAGLVAKKACELGLEVKPWVKTSLTPGSVVATEYLKHSGLQDYLNQQGFHVAAHGCATCVGNSGDLDGSVSAAITENDMVAAAVLSANRNFEGRVNPLTRANYLASPPLVVAYALAGTVDIDFEKEPIGVGKGGKEVFLRDIWPSNQEIDEVVESSVQTHLFKKVYDSIMERNPRWNQLPVPKEALYPWEDRSTYIRKPTYLEGMSMTPPAAPPTVTEAYCLLNLGDSITTDHISYSGKIPEGTPAAKYLLEYGVEPKNFSSYGGRRGNNEVVMRGAFANMRIVNKLLDGKAGPWTIHVPTGEKLYVYDAAMKYKSEGHDMVIIAGSEYGSGSSRDSAAKGPMLLGVKSVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGRERYTIHLPTSTAELSPGQDVTVTTHDGRSFTCTLRLDTQVLSAPRI

Target ORF information:

RefSeq Version	NM_001358373.1
Organism	Zea mays
Definition	Zea mays aconitase 2 (LOC100281040), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358373.1

ORF Insert Sequence:

ATGGCCAACC CAACAGCTAC CAAGCATGCT TTCAAGCGTA TTTTGACGAG CCTGCTTAAG 
CCTGGCGGTG GTGAATACGG CAAGTTCTTC AGCCTTCCGG CGCTAAATGA TCCAAGGATC 
GATAAGCTTC CCTACTCTAT ACGTGTTCTC CTGGAATCAG CTATCCGCCA TTGTGATAAC 
TTCCAAGTTA CAGAGAGTGA TGTTGAGAAA ATCATTGATT GGGAGAACAC ATCTCCAAAG 
CTGGCTGAGA TACCTTTCAA GCCAGCGAGA TGCATTCTAA TGGACAACAC TGGAGTCCCA 
GCAGTTGTAG ACCTTGCAGC TATGCGTGAT ATGATGCCAA AGTTGGGATG TGATCCCTAC 
AAGATTAATC CATTGATTCC CGTGGATGCT GTTATTGACC ACGCAGTGCG AGTGGATGTA 
GCAGGGACCT ATGATGCATT GGATAGAAAC GAGGAGCTGG AGTTCCAACG CAACAAAGAA 
AGGTTTGCTT TTCTTAAATG GGCATCCAAT GCTTTCCACA ACATGCAGGT TTTCCCTCCT 
GGTTCGGGCA CTGTACACCA GGTAAATCTT GAGTATCTTG CCCGAGTTGT CTTCAACGAA 
GATGGCATTC TTTACTTCGA CAGCGTGGTT GGCACAGACT CACACACCAC TATGATTAAT 
AGTCTTGGAG TTGCTGGGTG GGGAGTAGGC GGTATTGAAG CAGTCGTTGC AATGCTTGGC 
CAGCCAATGG GCATGGTTTT GCCTGGTGTG GTTGGATTCA AATTGAGTGG GAAGTTACGG 
GATGGTGTCA CTACTACTGA CATTGTTCTT ACCATGACCC AAATGCTAAG GAAGCACGGT 
GCTATTGGCA AATTCGTTGA ATTCTATGGT GTTGGTGTGG GCGAGCTATC TTTGCCTGCT 
AGGGCCACAA TTGCCAACAT GTCTCCAGAA TATGGAGCTA CCATGGGCTT CTTCCCTGTA 
GACCAAGTAG CATTGGACTA TCTCAAACTG ACAGGCCGAA GCGATGAAAC TGTGTCGATG 
ATTGAAGCAT ACCTGCGAGC TAACAAAATG TTTGTGGACA AGCATGAGCC TGAGACGGAA 
CGCGTTTTCT CTTCACATCT GGAGCTGGAC CTTAGTGAGG TGGAGCCTTG CGTTTCAGGC 
CCCAAAAGGC CTCATGATCG TGTCCCTTTG AAGGAAATGA AGTCAGACTG GCATGCTTGC 
CTGGACAACG AAGTTGGCTT CAAGGGCTAT GCGGTGCCAA AGGAACAGCA AGGCAAAGTT 
GTGAAATTCG ACTTCCATGG AAGGCCAGCT GAAATCAAGC ATGGCAGTGT TGTTCTTGCA 
GCAATATGTA GCTCCACAAA CACATCAAAT CCCAGCGTCA TGATTGGTGC TGGCCTTGTG 
GCAAAGAAAG CCTGCGAATT AGGCCTTGAG GTGAAGCCAT GGGTAAAGAC AAGCCTTACC 
CCTGGATCTG TGGTTGCTAC CGAGTACTTG AAACATAGTG GCCTTCAAGA TTATCTGAAC 
CAGCAAGGGT TCCATGTCGC TGCGCATGGT TGCGCCACTT GTGTGGGCAA CTCCGGTGAC 
CTGGATGGAT CTGTATCAGC TGCTATCACA GAAAATGATA TGGTTGCTGC TGCGGTGCTG 
TCGGCCAACC GAAACTTCGA GGGGCGTGTG AACCCTCTCA CTCGGGCTAA CTACCTTGCC 
TCGCCGCCTC TGGTTGTTGC CTATGCTCTT GCCGGCACTG TTGACATTGA CTTTGAGAAA 
GAGCCCATTG GAGTTGGAAA GGGTGGTAAG GAAGTTTTCT TGAGGGACAT ATGGCCTTCA 
AACCAAGAGA TCGATGAGGT TGTGGAGTCC AGCGTGCAGA CTCACCTGTT CAAGAAAGTG 
TACGACTCAA TCATGGAACG TAACCCTAGG TGGAACCAGC TGCCGGTTCC AAAGGAGGCA 
CTGTACCCAT GGGAAGACAG GTCCACCTAC ATCCGCAAGC CTACGTACCT GGAGGGCATG 
TCCATGACGC CGCCTGCAGC CCCGCCCACG GTGACGGAGG CCTACTGCCT GCTCAACCTT 
GGGGACAGCA TCACCACGGA CCACATCTCC TACTCGGGGA AGATCCCCGA GGGCACACCT 
GCGGCCAAGT ATCTGCTCGA GTATGGCGTG GAGCCCAAGA ACTTCAGCTC CTATGGTGGC 
CGCCGCGGGA ACAACGAGGT GGTGATGAGG GGAGCGTTTG CCAATATGAG GATCGTGAAT 
AAGCTTCTTG ATGGCAAGGC TGGTCCCTGG ACGATCCATG TTCCTACCGG GGAGAAGCTC 
TATGTTTACG ACGCGGCAAT GAAGTACAAG TCGGAAGGTC ATGACATGGT CATCATTGCT 
GGCTCCGAGT ACGGCAGTGG CAGCTCTCGT GACTCTGCTG CCAAGGGACC AATGCTACTG 
GGTGTCAAGT CGGTGATTGC AAAGAGCTTC GAGCGGATCC ACCGGAGCAA CTTGGTGGGG 
ATGGGGATCA TTCCTCTCTG CTTTAAAGCA GGAGAGGATG CCGATTCACT CGGCCTCACC 
GGACGTGAGC GCTACACCAT CCACCTCCCT ACCAGCACCG CCGAGCTCAG TCCAGGCCAG 
GACGTGACCG TCACAACCCA CGACGGCAGA TCCTTCACTT GCACTCTTCG CTTGGACACA 
CAGGTACTCA GTGCCCCACG TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100281040 ( XM_008663662.2 ) cDNA ORF clone, Zea mays -> XP_008661884.2 Zea mays aconitase 2 (aco2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OZa56261
Clone ID Related Accession (Same CDS sequence)	XM_008663662.2 , NM_001358373.1
Accession Version	XM_008663662.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1489852800000
Organism	Zea mays
Product	aconitase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024468.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2017 this sequence version replaced XM_008663662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACC CAACAGCTAC CAAGCATGCT TTCAAGCGTA TTTTGACGAG CCTGCTTAAG 
CCTGGCGGTG GTGAATACGG CAAGTTCTTC AGCCTTCCGG CGCTAAATGA TCCAAGGATC 
GATAAGCTTC CCTACTCTAT ACGTGTTCTC CTGGAATCAG CTATCCGCCA TTGTGATAAC 
TTCCAAGTTA CAGAGAGTGA TGTTGAGAAA ATCATTGATT GGGAGAACAC ATCTCCAAAG 
CTGGCTGAGA TACCTTTCAA GCCAGCGAGA TGCATTCTAA TGGACAACAC TGGAGTCCCA 
GCAGTTGTAG ACCTTGCAGC TATGCGTGAT ATGATGCCAA AGTTGGGATG TGATCCCTAC 
AAGATTAATC CATTGATTCC CGTGGATGCT GTTATTGACC ACGCAGTGCG AGTGGATGTA 
GCAGGGACCT ATGATGCATT GGATAGAAAC GAGGAGCTGG AGTTCCAACG CAACAAAGAA 
AGGTTTGCTT TTCTTAAATG GGCATCCAAT GCTTTCCACA ACATGCAGGT TTTCCCTCCT 
GGTTCGGGCA CTGTACACCA GGTAAATCTT GAGTATCTTG CCCGAGTTGT CTTCAACGAA 
GATGGCATTC TTTACTTCGA CAGCGTGGTT GGCACAGACT CACACACCAC TATGATTAAT 
AGTCTTGGAG TTGCTGGGTG GGGAGTAGGC GGTATTGAAG CAGTCGTTGC AATGCTTGGC 
CAGCCAATGG GCATGGTTTT GCCTGGTGTG GTTGGATTCA AATTGAGTGG GAAGTTACGG 
GATGGTGTCA CTACTACTGA CATTGTTCTT ACCATGACCC AAATGCTAAG GAAGCACGGT 
GCTATTGGCA AATTCGTTGA ATTCTATGGT GTTGGTGTGG GCGAGCTATC TTTGCCTGCT 
AGGGCCACAA TTGCCAACAT GTCTCCAGAA TATGGAGCTA CCATGGGCTT CTTCCCTGTA 
GACCAAGTAG CATTGGACTA TCTCAAACTG ACAGGCCGAA GCGATGAAAC TGTGTCGATG 
ATTGAAGCAT ACCTGCGAGC TAACAAAATG TTTGTGGACA AGCATGAGCC TGAGACGGAA 
CGCGTTTTCT CTTCACATCT GGAGCTGGAC CTTAGTGAGG TGGAGCCTTG CGTTTCAGGC 
CCCAAAAGGC CTCATGATCG TGTCCCTTTG AAGGAAATGA AGTCAGACTG GCATGCTTGC 
CTGGACAACG AAGTTGGCTT CAAGGGCTAT GCGGTGCCAA AGGAACAGCA AGGCAAAGTT 
GTGAAATTCG ACTTCCATGG AAGGCCAGCT GAAATCAAGC ATGGCAGTGT TGTTCTTGCA 
GCAATATGTA GCTCCACAAA CACATCAAAT CCCAGCGTCA TGATTGGTGC TGGCCTTGTG 
GCAAAGAAAG CCTGCGAATT AGGCCTTGAG GTGAAGCCAT GGGTAAAGAC AAGCCTTACC 
CCTGGATCTG TGGTTGCTAC CGAGTACTTG AAACATAGTG GCCTTCAAGA TTATCTGAAC 
CAGCAAGGGT TCCATGTCGC TGCGCATGGT TGCGCCACTT GTGTGGGCAA CTCCGGTGAC 
CTGGATGGAT CTGTATCAGC TGCTATCACA GAAAATGATA TGGTTGCTGC TGCGGTGCTG 
TCGGCCAACC GAAACTTCGA GGGGCGTGTG AACCCTCTCA CTCGGGCTAA CTACCTTGCC 
TCGCCGCCTC TGGTTGTTGC CTATGCTCTT GCCGGCACTG TTGACATTGA CTTTGAGAAA 
GAGCCCATTG GAGTTGGAAA GGGTGGTAAG GAAGTTTTCT TGAGGGACAT ATGGCCTTCA 
AACCAAGAGA TCGATGAGGT TGTGGAGTCC AGCGTGCAGA CTCACCTGTT CAAGAAAGTG 
TACGACTCAA TCATGGAACG TAACCCTAGG TGGAACCAGC TGCCGGTTCC AAAGGAGGCA 
CTGTACCCAT GGGAAGACAG GTCCACCTAC ATCCGCAAGC CTACGTACCT GGAGGGCATG 
TCCATGACGC CGCCTGCAGC CCCGCCCACG GTGACGGAGG CCTACTGCCT GCTCAACCTT 
GGGGACAGCA TCACCACGGA CCACATCTCC TACTCGGGGA AGATCCCCGA GGGCACACCT 
GCGGCCAAGT ATCTGCTCGA GTATGGCGTG GAGCCCAAGA ACTTCAGCTC CTATGGTGGC 
CGCCGCGGGA ACAACGAGGT GGTGATGAGG GGAGCGTTTG CCAATATGAG GATCGTGAAT 
AAGCTTCTTG ATGGCAAGGC TGGTCCCTGG ACGATCCATG TTCCTACCGG GGAGAAGCTC 
TATGTTTACG ACGCGGCAAT GAAGTACAAG TCGGAAGGTC ATGACATGGT CATCATTGCT 
GGCTCCGAGT ACGGCAGTGG CAGCTCTCGT GACTCTGCTG CCAAGGGACC AATGCTACTG 
GGTGTCAAGT CGGTGATTGC AAAGAGCTTC GAGCGGATCC ACCGGAGCAA CTTGGTGGGG 
ATGGGGATCA TTCCTCTCTG CTTTAAAGCA GGAGAGGATG CCGATTCACT CGGCCTCACC 
GGACGTGAGC GCTACACCAT CCACCTCCCT ACCAGCACCG CCGAGCTCAG TCCAGGCCAG 
GACGTGACCG TCACAACCCA CGACGGCAGA TCCTTCACTT GCACTCTTCG CTTGGACACA 
CAGGTACTCA GTGCCCCACG TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008661884.2
CDS	143..2809
Translation	MANPTATKHAFKRILTSLLKPGGGEYGKFFSLPALNDPRIDKLPYSIRVLLESAIRHCDNFQVTESDVEKIIDWENTSPKLAEIPFKPARCILMDNTGVPAVVDLAAMRDMMPKLGCDPYKINPLIPVDAVIDHAVRVDVAGTYDALDRNEELEFQRNKERFAFLKWASNAFHNMQVFPPGSGTVHQVNLEYLARVVFNEDGILYFDSVVGTDSHTTMINSLGVAGWGVGGIEAVVAMLGQPMGMVLPGVVGFKLSGKLRDGVTTTDIVLTMTQMLRKHGAIGKFVEFYGVGVGELSLPARATIANMSPEYGATMGFFPVDQVALDYLKLTGRSDETVSMIEAYLRANKMFVDKHEPETERVFSSHLELDLSEVEPCVSGPKRPHDRVPLKEMKSDWHACLDNEVGFKGYAVPKEQQGKVVKFDFHGRPAEIKHGSVVLAAICSSTNTSNPSVMIGAGLVAKKACELGLEVKPWVKTSLTPGSVVATEYLKHSGLQDYLNQQGFHVAAHGCATCVGNSGDLDGSVSAAITENDMVAAAVLSANRNFEGRVNPLTRANYLASPPLVVAYALAGTVDIDFEKEPIGVGKGGKEVFLRDIWPSNQEIDEVVESSVQTHLFKKVYDSIMERNPRWNQLPVPKEALYPWEDRSTYIRKPTYLEGMSMTPPAAPPTVTEAYCLLNLGDSITTDHISYSGKIPEGTPAAKYLLEYGVEPKNFSSYGGRRGNNEVVMRGAFANMRIVNKLLDGKAGPWTIHVPTGEKLYVYDAAMKYKSEGHDMVIIAGSEYGSGSSRDSAAKGPMLLGVKSVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGRERYTIHLPTSTAELSPGQDVTVTTHDGRSFTCTLRLDTQVLSAPRI

Target ORF information:

RefSeq Version	XM_008663662.2
Organism	Zea mays
Definition	Zea mays aconitase 2 (aco2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008663662.2

ORF Insert Sequence:

ATGGCCAACC CAACAGCTAC CAAGCATGCT TTCAAGCGTA TTTTGACGAG CCTGCTTAAG 
CCTGGCGGTG GTGAATACGG CAAGTTCTTC AGCCTTCCGG CGCTAAATGA TCCAAGGATC 
GATAAGCTTC CCTACTCTAT ACGTGTTCTC CTGGAATCAG CTATCCGCCA TTGTGATAAC 
TTCCAAGTTA CAGAGAGTGA TGTTGAGAAA ATCATTGATT GGGAGAACAC ATCTCCAAAG 
CTGGCTGAGA TACCTTTCAA GCCAGCGAGA TGCATTCTAA TGGACAACAC TGGAGTCCCA 
GCAGTTGTAG ACCTTGCAGC TATGCGTGAT ATGATGCCAA AGTTGGGATG TGATCCCTAC 
AAGATTAATC CATTGATTCC CGTGGATGCT GTTATTGACC ACGCAGTGCG AGTGGATGTA 
GCAGGGACCT ATGATGCATT GGATAGAAAC GAGGAGCTGG AGTTCCAACG CAACAAAGAA 
AGGTTTGCTT TTCTTAAATG GGCATCCAAT GCTTTCCACA ACATGCAGGT TTTCCCTCCT 
GGTTCGGGCA CTGTACACCA GGTAAATCTT GAGTATCTTG CCCGAGTTGT CTTCAACGAA 
GATGGCATTC TTTACTTCGA CAGCGTGGTT GGCACAGACT CACACACCAC TATGATTAAT 
AGTCTTGGAG TTGCTGGGTG GGGAGTAGGC GGTATTGAAG CAGTCGTTGC AATGCTTGGC 
CAGCCAATGG GCATGGTTTT GCCTGGTGTG GTTGGATTCA AATTGAGTGG GAAGTTACGG 
GATGGTGTCA CTACTACTGA CATTGTTCTT ACCATGACCC AAATGCTAAG GAAGCACGGT 
GCTATTGGCA AATTCGTTGA ATTCTATGGT GTTGGTGTGG GCGAGCTATC TTTGCCTGCT 
AGGGCCACAA TTGCCAACAT GTCTCCAGAA TATGGAGCTA CCATGGGCTT CTTCCCTGTA 
GACCAAGTAG CATTGGACTA TCTCAAACTG ACAGGCCGAA GCGATGAAAC TGTGTCGATG 
ATTGAAGCAT ACCTGCGAGC TAACAAAATG TTTGTGGACA AGCATGAGCC TGAGACGGAA 
CGCGTTTTCT CTTCACATCT GGAGCTGGAC CTTAGTGAGG TGGAGCCTTG CGTTTCAGGC 
CCCAAAAGGC CTCATGATCG TGTCCCTTTG AAGGAAATGA AGTCAGACTG GCATGCTTGC 
CTGGACAACG AAGTTGGCTT CAAGGGCTAT GCGGTGCCAA AGGAACAGCA AGGCAAAGTT 
GTGAAATTCG ACTTCCATGG AAGGCCAGCT GAAATCAAGC ATGGCAGTGT TGTTCTTGCA 
GCAATATGTA GCTCCACAAA CACATCAAAT CCCAGCGTCA TGATTGGTGC TGGCCTTGTG 
GCAAAGAAAG CCTGCGAATT AGGCCTTGAG GTGAAGCCAT GGGTAAAGAC AAGCCTTACC 
CCTGGATCTG TGGTTGCTAC CGAGTACTTG AAACATAGTG GCCTTCAAGA TTATCTGAAC 
CAGCAAGGGT TCCATGTCGC TGCGCATGGT TGCGCCACTT GTGTGGGCAA CTCCGGTGAC 
CTGGATGGAT CTGTATCAGC TGCTATCACA GAAAATGATA TGGTTGCTGC TGCGGTGCTG 
TCGGCCAACC GAAACTTCGA GGGGCGTGTG AACCCTCTCA CTCGGGCTAA CTACCTTGCC 
TCGCCGCCTC TGGTTGTTGC CTATGCTCTT GCCGGCACTG TTGACATTGA CTTTGAGAAA 
GAGCCCATTG GAGTTGGAAA GGGTGGTAAG GAAGTTTTCT TGAGGGACAT ATGGCCTTCA 
AACCAAGAGA TCGATGAGGT TGTGGAGTCC AGCGTGCAGA CTCACCTGTT CAAGAAAGTG 
TACGACTCAA TCATGGAACG TAACCCTAGG TGGAACCAGC TGCCGGTTCC AAAGGAGGCA 
CTGTACCCAT GGGAAGACAG GTCCACCTAC ATCCGCAAGC CTACGTACCT GGAGGGCATG 
TCCATGACGC CGCCTGCAGC CCCGCCCACG GTGACGGAGG CCTACTGCCT GCTCAACCTT 
GGGGACAGCA TCACCACGGA CCACATCTCC TACTCGGGGA AGATCCCCGA GGGCACACCT 
GCGGCCAAGT ATCTGCTCGA GTATGGCGTG GAGCCCAAGA ACTTCAGCTC CTATGGTGGC 
CGCCGCGGGA ACAACGAGGT GGTGATGAGG GGAGCGTTTG CCAATATGAG GATCGTGAAT 
AAGCTTCTTG ATGGCAAGGC TGGTCCCTGG ACGATCCATG TTCCTACCGG GGAGAAGCTC 
TATGTTTACG ACGCGGCAAT GAAGTACAAG TCGGAAGGTC ATGACATGGT CATCATTGCT 
GGCTCCGAGT ACGGCAGTGG CAGCTCTCGT GACTCTGCTG CCAAGGGACC AATGCTACTG 
GGTGTCAAGT CGGTGATTGC AAAGAGCTTC GAGCGGATCC ACCGGAGCAA CTTGGTGGGG 
ATGGGGATCA TTCCTCTCTG CTTTAAAGCA GGAGAGGATG CCGATTCACT CGGCCTCACC 
GGACGTGAGC GCTACACCAT CCACCTCCCT ACCAGCACCG CCGAGCTCAG TCCAGGCCAG 
GACGTGACCG TCACAACCCA CGACGGCAGA TCCTTCACTT GCACTCTTCG CTTGGACACA 
CAGGTACTCA GTGCCCCACG TATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100281040 ( XM_020545034.1 ) cDNA ORF clone, Zea mays -> XP_020400623.1 Zea mays aconitase 2 (aco2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OZa56262
Clone ID Related Accession (Same CDS sequence)	XM_020545034.1 , XM_020545034.2
Accession Version	XM_020545034.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1489852800000
Organism	Zea mays
Product	aconitase 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024468.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACC CAACAGCTAC CAAGCATGCT TTCAAGCGTA TTTTGACGAG CCTGCTTAAG 
CCTGGCGGTG GTGAATACGG CAAGTTCTTC AGCCTTCCGG CGCTAAATGA TCCAAGGATC 
GATAAGCTTC CCTACTCTAT ACGTGTTCTC CTGGAATCAG CTATCCGCCA TTGTGATAAC 
TTCCAAGTTA CAGAGAGTGA TGTTGAGAAA ATCATTGATT GGGAGAACAC ATCTCCAAAG 
CTGGCTGAGA TACCTTTCAA GCCAGCGAGA TGCATTCTAA TGGACAACAC TGGAGTCCCA 
GCAGTTGTAG ACCTTGCAGC TATGCGTGAT ATGATGCCAA AGTTGGGATG TGATCCCTAC 
AAGATTAATC CATTGATTCC CGTGGATGCT GTTATTGACC ACGCAGTGCG AGTGGATGTA 
GCAGGGACCT ATGATGCATT GGATAGAAAC GAGGAGCTGG AGTTCCAACG CAACAAAGAA 
AGGTTTGCTT TTCTTAAATG GGCATCCAAT GCTTTCCACA ACATGCAGGT TTTCCCTCCT 
GGTTCGGGCA CTGTACACCA GGTAAATCTT GAGTATCTTG CCCGAGTTGT CTTCAACGAA 
GATGGCATTC TTTACTTCGA CAGCGTGGTT GGCACAGACT CACACACCAC TATGATTAAT 
AGTCTTGGAG TTGCTGGGTG GGGAGTAGGC GGTATTGAAG CAGTCGTTGC AATGCTTGGC 
CAGCCAATGG GCATGGTTTT GCCTGGTGTG GTTGGATTCA AATTGAGTGG GAAGTTACGG 
GATGGTGTCA CTACTACTGA CATTGTTCTT ACCATGACCC AAATGCTAAG GAAGCACGGT 
GCTATTGGCA AATTCGTTGA ATTCTATGGT GTTGGTGTGG GCGAGCTATC TTTGCCTGCT 
AGGGCCACAA TTGCCAACAT GTCTCCAGAA TATGGAGCTA CCATGGGCTT CTTCCCTGTA 
GACCAAGTAG CATTGGACTA TCTCAAACTG ACAGGCCGAA GCGATGAAAC TGTGTCGATG 
ATTGAAGCAT ACCTGCGAGC TAACAAAATG TTTGTGGACA AGCATGAGCC TGAGACGGAA 
CGCGTTTTCT CTTCACATCT GGAGCTGGAC CTTAGTGAGG TGGAGCCTTG CGTTTCAGGC 
CCCAAAAGGC CTCATGATCG TGTCCCTTTG AAGGAAATGA AGTCAGACTG GCATGCTTGC 
CTGGACAACG AAGTTGGCTT CAAGGGCTAT GCGGTGCCAA AGGAACAGCA AGGCAAAGTT 
GTGAAATTCG ACTTCCATGG AAGGCCAGCT GAAATCAAGC ATGGCAGTGT TGTTCTTGCA 
GCAATATGTA GCTCCACAAA CACATCAAAT CCCAGCGTCA TGATTGGTGC TGGCCTTGTG 
GCAAAGAAAG CCTGCGAATT AGGCCTTGAG GTGAAGCCAT GGGTAAAGAC AAGCCTTACC 
CCTGGATCTG TGGTTGCTAC CGAGTACTTG AAACATAGTG GCCTTCAAGA TTATCTGAAC 
CAGCAAGGGT TCCATGTCGC TGCGCATGGT TGCGCCACTT GTGTGGGCAA CTCCGGTGAC 
CTGGATGGAT CTGTATCAGC TGCTATCACA GAAAATGATA TGGTTGCTGC TGCGGTGCTG 
TCGGCCAACC GAAACTTCGA GGGGCGTGTG AACCCTCTCA CTCGGGCTAA CTACCTTGCC 
TCGCCGCCTC TGGTTGTTGC CTATGCTCTT GCCGGCACTG TTGACATTGA CTTTGAGAAA 
GAGCCCATTG GAGTTGGAAA GGGTGGTAAG GAAGTTTTCT TGAGGGACAT ATGGCCTTCA 
AACCAAGAGA TCGATGAGGT TGTGGAGTCC AGCGTGCAGA CTCACCTGTT CAAGAAAGTG 
TACGACTCAA TCATGGAACG TAACCCTAGG TGGAACCAGC TGCCGGTTCC AAAGGAGGCA 
CTGTACCCAT GGGAAGACAG GTCCACCTAC ATCCGCAAGC CTACGTACCT GGAGGGCATG 
TCCATGACGC CGCCTGCAGC CCCGCCCACG GTGACGGAGG CCTACTGCCT GCTCAACCTT 
GGGGACAGCA TCACCACGGA CCACATCTCC TACTCGGGGA AGATCCCCGA GGGCACACCT 
GCGGCCAAGT ATCTGCTCGA GTATGGCGTG GAGCCCAAGA ACTTCAGCTC CTATGGTGGC 
CGCCGCGGGA ACAACGAGGT GGTGATGAGG GGAGCGTTTG CCAATATGAG GATCGTGAAT 
AAGCTTCTTG ATGGCAAGGC TGGTCCCTGG ACGATCCATG TTCCTACCGG GGAGAAGCTC 
TATGTTTACG ACGCGGCAAT GAAGTACAAG TCGGAAGGTC ATGACATGGT CATCATTGCT 
GGCTCCGAGT ACGGCAGTGG CAGCTCTCGT GACTCTGCTG CCAAGGGACC AATGCTACTG 
GGTGTCAAGT CGGTGATTGC AAAGAGCTTC GAGCGGATCC ACCGGAGCAA CTTGGTGGGG 
ATGGGGATCA TTCCTCTCTG CTTTAAAGCA GGAGAGGATG CCGATTCACT CGGCCTCACC 
GGACGTGAGC GCTACACCAT CCACCTCCCT ACCAGCACCG CCGAGCTCAG TCCAGGCCAG 
GACGTGACCG TCACAACCCA CGACGGCAGA TCCTTCACTT GCACTCTTCG CTTGGACACA 
CAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020400623.1
CDS	143..2791
Translation	MANPTATKHAFKRILTSLLKPGGGEYGKFFSLPALNDPRIDKLPYSIRVLLESAIRHCDNFQVTESDVEKIIDWENTSPKLAEIPFKPARCILMDNTGVPAVVDLAAMRDMMPKLGCDPYKINPLIPVDAVIDHAVRVDVAGTYDALDRNEELEFQRNKERFAFLKWASNAFHNMQVFPPGSGTVHQVNLEYLARVVFNEDGILYFDSVVGTDSHTTMINSLGVAGWGVGGIEAVVAMLGQPMGMVLPGVVGFKLSGKLRDGVTTTDIVLTMTQMLRKHGAIGKFVEFYGVGVGELSLPARATIANMSPEYGATMGFFPVDQVALDYLKLTGRSDETVSMIEAYLRANKMFVDKHEPETERVFSSHLELDLSEVEPCVSGPKRPHDRVPLKEMKSDWHACLDNEVGFKGYAVPKEQQGKVVKFDFHGRPAEIKHGSVVLAAICSSTNTSNPSVMIGAGLVAKKACELGLEVKPWVKTSLTPGSVVATEYLKHSGLQDYLNQQGFHVAAHGCATCVGNSGDLDGSVSAAITENDMVAAAVLSANRNFEGRVNPLTRANYLASPPLVVAYALAGTVDIDFEKEPIGVGKGGKEVFLRDIWPSNQEIDEVVESSVQTHLFKKVYDSIMERNPRWNQLPVPKEALYPWEDRSTYIRKPTYLEGMSMTPPAAPPTVTEAYCLLNLGDSITTDHISYSGKIPEGTPAAKYLLEYGVEPKNFSSYGGRRGNNEVVMRGAFANMRIVNKLLDGKAGPWTIHVPTGEKLYVYDAAMKYKSEGHDMVIIAGSEYGSGSSRDSAAKGPMLLGVKSVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGRERYTIHLPTSTAELSPGQDVTVTTHDGRSFTCTLRLDTQV

Target ORF information:

RefSeq Version	XM_020545034.1
Organism	Zea mays
Definition	Zea mays aconitase 2 (aco2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020545034.1

ORF Insert Sequence:

ATGGCCAACC CAACAGCTAC CAAGCATGCT TTCAAGCGTA TTTTGACGAG CCTGCTTAAG 
CCTGGCGGTG GTGAATACGG CAAGTTCTTC AGCCTTCCGG CGCTAAATGA TCCAAGGATC 
GATAAGCTTC CCTACTCTAT ACGTGTTCTC CTGGAATCAG CTATCCGCCA TTGTGATAAC 
TTCCAAGTTA CAGAGAGTGA TGTTGAGAAA ATCATTGATT GGGAGAACAC ATCTCCAAAG 
CTGGCTGAGA TACCTTTCAA GCCAGCGAGA TGCATTCTAA TGGACAACAC TGGAGTCCCA 
GCAGTTGTAG ACCTTGCAGC TATGCGTGAT ATGATGCCAA AGTTGGGATG TGATCCCTAC 
AAGATTAATC CATTGATTCC CGTGGATGCT GTTATTGACC ACGCAGTGCG AGTGGATGTA 
GCAGGGACCT ATGATGCATT GGATAGAAAC GAGGAGCTGG AGTTCCAACG CAACAAAGAA 
AGGTTTGCTT TTCTTAAATG GGCATCCAAT GCTTTCCACA ACATGCAGGT TTTCCCTCCT 
GGTTCGGGCA CTGTACACCA GGTAAATCTT GAGTATCTTG CCCGAGTTGT CTTCAACGAA 
GATGGCATTC TTTACTTCGA CAGCGTGGTT GGCACAGACT CACACACCAC TATGATTAAT 
AGTCTTGGAG TTGCTGGGTG GGGAGTAGGC GGTATTGAAG CAGTCGTTGC AATGCTTGGC 
CAGCCAATGG GCATGGTTTT GCCTGGTGTG GTTGGATTCA AATTGAGTGG GAAGTTACGG 
GATGGTGTCA CTACTACTGA CATTGTTCTT ACCATGACCC AAATGCTAAG GAAGCACGGT 
GCTATTGGCA AATTCGTTGA ATTCTATGGT GTTGGTGTGG GCGAGCTATC TTTGCCTGCT 
AGGGCCACAA TTGCCAACAT GTCTCCAGAA TATGGAGCTA CCATGGGCTT CTTCCCTGTA 
GACCAAGTAG CATTGGACTA TCTCAAACTG ACAGGCCGAA GCGATGAAAC TGTGTCGATG 
ATTGAAGCAT ACCTGCGAGC TAACAAAATG TTTGTGGACA AGCATGAGCC TGAGACGGAA 
CGCGTTTTCT CTTCACATCT GGAGCTGGAC CTTAGTGAGG TGGAGCCTTG CGTTTCAGGC 
CCCAAAAGGC CTCATGATCG TGTCCCTTTG AAGGAAATGA AGTCAGACTG GCATGCTTGC 
CTGGACAACG AAGTTGGCTT CAAGGGCTAT GCGGTGCCAA AGGAACAGCA AGGCAAAGTT 
GTGAAATTCG ACTTCCATGG AAGGCCAGCT GAAATCAAGC ATGGCAGTGT TGTTCTTGCA 
GCAATATGTA GCTCCACAAA CACATCAAAT CCCAGCGTCA TGATTGGTGC TGGCCTTGTG 
GCAAAGAAAG CCTGCGAATT AGGCCTTGAG GTGAAGCCAT GGGTAAAGAC AAGCCTTACC 
CCTGGATCTG TGGTTGCTAC CGAGTACTTG AAACATAGTG GCCTTCAAGA TTATCTGAAC 
CAGCAAGGGT TCCATGTCGC TGCGCATGGT TGCGCCACTT GTGTGGGCAA CTCCGGTGAC 
CTGGATGGAT CTGTATCAGC TGCTATCACA GAAAATGATA TGGTTGCTGC TGCGGTGCTG 
TCGGCCAACC GAAACTTCGA GGGGCGTGTG AACCCTCTCA CTCGGGCTAA CTACCTTGCC 
TCGCCGCCTC TGGTTGTTGC CTATGCTCTT GCCGGCACTG TTGACATTGA CTTTGAGAAA 
GAGCCCATTG GAGTTGGAAA GGGTGGTAAG GAAGTTTTCT TGAGGGACAT ATGGCCTTCA 
AACCAAGAGA TCGATGAGGT TGTGGAGTCC AGCGTGCAGA CTCACCTGTT CAAGAAAGTG 
TACGACTCAA TCATGGAACG TAACCCTAGG TGGAACCAGC TGCCGGTTCC AAAGGAGGCA 
CTGTACCCAT GGGAAGACAG GTCCACCTAC ATCCGCAAGC CTACGTACCT GGAGGGCATG 
TCCATGACGC CGCCTGCAGC CCCGCCCACG GTGACGGAGG CCTACTGCCT GCTCAACCTT 
GGGGACAGCA TCACCACGGA CCACATCTCC TACTCGGGGA AGATCCCCGA GGGCACACCT 
GCGGCCAAGT ATCTGCTCGA GTATGGCGTG GAGCCCAAGA ACTTCAGCTC CTATGGTGGC 
CGCCGCGGGA ACAACGAGGT GGTGATGAGG GGAGCGTTTG CCAATATGAG GATCGTGAAT 
AAGCTTCTTG ATGGCAAGGC TGGTCCCTGG ACGATCCATG TTCCTACCGG GGAGAAGCTC 
TATGTTTACG ACGCGGCAAT GAAGTACAAG TCGGAAGGTC ATGACATGGT CATCATTGCT 
GGCTCCGAGT ACGGCAGTGG CAGCTCTCGT GACTCTGCTG CCAAGGGACC AATGCTACTG 
GGTGTCAAGT CGGTGATTGC AAAGAGCTTC GAGCGGATCC ACCGGAGCAA CTTGGTGGGG 
ATGGGGATCA TTCCTCTCTG CTTTAAAGCA GGAGAGGATG CCGATTCACT CGGCCTCACC 
GGACGTGAGC GCTACACCAT CCACCTCCCT ACCAGCACCG CCGAGCTCAG TCCAGGCCAG 
GACGTGACCG TCACAACCCA CGACGGCAGA TCCTTCACTT GCACTCTTCG CTTGGACACA 
CAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100281040 ( XM_020545034.2 ) cDNA ORF clone, Zea mays -> XP_020400623.1 Zea mays aconitase 2 (aco2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OZa56262
Clone ID Related Accession (Same CDS sequence)	XM_020545034.1 , XM_020545034.2
Accession Version	XM_020545034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	aconitase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024468.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2017 this sequence version replaced XM_020545034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAACC CAACAGCTAC CAAGCATGCT TTCAAGCGTA TTTTGACGAG CCTGCTTAAG 
CCTGGCGGTG GTGAATACGG CAAGTTCTTC AGCCTTCCGG CGCTAAATGA TCCAAGGATC 
GATAAGCTTC CCTACTCTAT ACGTGTTCTC CTGGAATCAG CTATCCGCCA TTGTGATAAC 
TTCCAAGTTA CAGAGAGTGA TGTTGAGAAA ATCATTGATT GGGAGAACAC ATCTCCAAAG 
CTGGCTGAGA TACCTTTCAA GCCAGCGAGA TGCATTCTAA TGGACAACAC TGGAGTCCCA 
GCAGTTGTAG ACCTTGCAGC TATGCGTGAT ATGATGCCAA AGTTGGGATG TGATCCCTAC 
AAGATTAATC CATTGATTCC CGTGGATGCT GTTATTGACC ACGCAGTGCG AGTGGATGTA 
GCAGGGACCT ATGATGCATT GGATAGAAAC GAGGAGCTGG AGTTCCAACG CAACAAAGAA 
AGGTTTGCTT TTCTTAAATG GGCATCCAAT GCTTTCCACA ACATGCAGGT TTTCCCTCCT 
GGTTCGGGCA CTGTACACCA GGTAAATCTT GAGTATCTTG CCCGAGTTGT CTTCAACGAA 
GATGGCATTC TTTACTTCGA CAGCGTGGTT GGCACAGACT CACACACCAC TATGATTAAT 
AGTCTTGGAG TTGCTGGGTG GGGAGTAGGC GGTATTGAAG CAGTCGTTGC AATGCTTGGC 
CAGCCAATGG GCATGGTTTT GCCTGGTGTG GTTGGATTCA AATTGAGTGG GAAGTTACGG 
GATGGTGTCA CTACTACTGA CATTGTTCTT ACCATGACCC AAATGCTAAG GAAGCACGGT 
GCTATTGGCA AATTCGTTGA ATTCTATGGT GTTGGTGTGG GCGAGCTATC TTTGCCTGCT 
AGGGCCACAA TTGCCAACAT GTCTCCAGAA TATGGAGCTA CCATGGGCTT CTTCCCTGTA 
GACCAAGTAG CATTGGACTA TCTCAAACTG ACAGGCCGAA GCGATGAAAC TGTGTCGATG 
ATTGAAGCAT ACCTGCGAGC TAACAAAATG TTTGTGGACA AGCATGAGCC TGAGACGGAA 
CGCGTTTTCT CTTCACATCT GGAGCTGGAC CTTAGTGAGG TGGAGCCTTG CGTTTCAGGC 
CCCAAAAGGC CTCATGATCG TGTCCCTTTG AAGGAAATGA AGTCAGACTG GCATGCTTGC 
CTGGACAACG AAGTTGGCTT CAAGGGCTAT GCGGTGCCAA AGGAACAGCA AGGCAAAGTT 
GTGAAATTCG ACTTCCATGG AAGGCCAGCT GAAATCAAGC ATGGCAGTGT TGTTCTTGCA 
GCAATATGTA GCTCCACAAA CACATCAAAT CCCAGCGTCA TGATTGGTGC TGGCCTTGTG 
GCAAAGAAAG CCTGCGAATT AGGCCTTGAG GTGAAGCCAT GGGTAAAGAC AAGCCTTACC 
CCTGGATCTG TGGTTGCTAC CGAGTACTTG AAACATAGTG GCCTTCAAGA TTATCTGAAC 
CAGCAAGGGT TCCATGTCGC TGCGCATGGT TGCGCCACTT GTGTGGGCAA CTCCGGTGAC 
CTGGATGGAT CTGTATCAGC TGCTATCACA GAAAATGATA TGGTTGCTGC TGCGGTGCTG 
TCGGCCAACC GAAACTTCGA GGGGCGTGTG AACCCTCTCA CTCGGGCTAA CTACCTTGCC 
TCGCCGCCTC TGGTTGTTGC CTATGCTCTT GCCGGCACTG TTGACATTGA CTTTGAGAAA 
GAGCCCATTG GAGTTGGAAA GGGTGGTAAG GAAGTTTTCT TGAGGGACAT ATGGCCTTCA 
AACCAAGAGA TCGATGAGGT TGTGGAGTCC AGCGTGCAGA CTCACCTGTT CAAGAAAGTG 
TACGACTCAA TCATGGAACG TAACCCTAGG TGGAACCAGC TGCCGGTTCC AAAGGAGGCA 
CTGTACCCAT GGGAAGACAG GTCCACCTAC ATCCGCAAGC CTACGTACCT GGAGGGCATG 
TCCATGACGC CGCCTGCAGC CCCGCCCACG GTGACGGAGG CCTACTGCCT GCTCAACCTT 
GGGGACAGCA TCACCACGGA CCACATCTCC TACTCGGGGA AGATCCCCGA GGGCACACCT 
GCGGCCAAGT ATCTGCTCGA GTATGGCGTG GAGCCCAAGA ACTTCAGCTC CTATGGTGGC 
CGCCGCGGGA ACAACGAGGT GGTGATGAGG GGAGCGTTTG CCAATATGAG GATCGTGAAT 
AAGCTTCTTG ATGGCAAGGC TGGTCCCTGG ACGATCCATG TTCCTACCGG GGAGAAGCTC 
TATGTTTACG ACGCGGCAAT GAAGTACAAG TCGGAAGGTC ATGACATGGT CATCATTGCT 
GGCTCCGAGT ACGGCAGTGG CAGCTCTCGT GACTCTGCTG CCAAGGGACC AATGCTACTG 
GGTGTCAAGT CGGTGATTGC AAAGAGCTTC GAGCGGATCC ACCGGAGCAA CTTGGTGGGG 
ATGGGGATCA TTCCTCTCTG CTTTAAAGCA GGAGAGGATG CCGATTCACT CGGCCTCACC 
GGACGTGAGC GCTACACCAT CCACCTCCCT ACCAGCACCG CCGAGCTCAG TCCAGGCCAG 
GACGTGACCG TCACAACCCA CGACGGCAGA TCCTTCACTT GCACTCTTCG CTTGGACACA 
CAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020400623.1
CDS	142..2790
Translation	MANPTATKHAFKRILTSLLKPGGGEYGKFFSLPALNDPRIDKLPYSIRVLLESAIRHCDNFQVTESDVEKIIDWENTSPKLAEIPFKPARCILMDNTGVPAVVDLAAMRDMMPKLGCDPYKINPLIPVDAVIDHAVRVDVAGTYDALDRNEELEFQRNKERFAFLKWASNAFHNMQVFPPGSGTVHQVNLEYLARVVFNEDGILYFDSVVGTDSHTTMINSLGVAGWGVGGIEAVVAMLGQPMGMVLPGVVGFKLSGKLRDGVTTTDIVLTMTQMLRKHGAIGKFVEFYGVGVGELSLPARATIANMSPEYGATMGFFPVDQVALDYLKLTGRSDETVSMIEAYLRANKMFVDKHEPETERVFSSHLELDLSEVEPCVSGPKRPHDRVPLKEMKSDWHACLDNEVGFKGYAVPKEQQGKVVKFDFHGRPAEIKHGSVVLAAICSSTNTSNPSVMIGAGLVAKKACELGLEVKPWVKTSLTPGSVVATEYLKHSGLQDYLNQQGFHVAAHGCATCVGNSGDLDGSVSAAITENDMVAAAVLSANRNFEGRVNPLTRANYLASPPLVVAYALAGTVDIDFEKEPIGVGKGGKEVFLRDIWPSNQEIDEVVESSVQTHLFKKVYDSIMERNPRWNQLPVPKEALYPWEDRSTYIRKPTYLEGMSMTPPAAPPTVTEAYCLLNLGDSITTDHISYSGKIPEGTPAAKYLLEYGVEPKNFSSYGGRRGNNEVVMRGAFANMRIVNKLLDGKAGPWTIHVPTGEKLYVYDAAMKYKSEGHDMVIIAGSEYGSGSSRDSAAKGPMLLGVKSVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGRERYTIHLPTSTAELSPGQDVTVTTHDGRSFTCTLRLDTQV

Target ORF information:

RefSeq Version	XM_020545034.2
Organism	Zea mays
Definition	Zea mays aconitase 2 (aco2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020545034.2

ORF Insert Sequence:

ATGGCCAACC CAACAGCTAC CAAGCATGCT TTCAAGCGTA TTTTGACGAG CCTGCTTAAG 
CCTGGCGGTG GTGAATACGG CAAGTTCTTC AGCCTTCCGG CGCTAAATGA TCCAAGGATC 
GATAAGCTTC CCTACTCTAT ACGTGTTCTC CTGGAATCAG CTATCCGCCA TTGTGATAAC 
TTCCAAGTTA CAGAGAGTGA TGTTGAGAAA ATCATTGATT GGGAGAACAC ATCTCCAAAG 
CTGGCTGAGA TACCTTTCAA GCCAGCGAGA TGCATTCTAA TGGACAACAC TGGAGTCCCA 
GCAGTTGTAG ACCTTGCAGC TATGCGTGAT ATGATGCCAA AGTTGGGATG TGATCCCTAC 
AAGATTAATC CATTGATTCC CGTGGATGCT GTTATTGACC ACGCAGTGCG AGTGGATGTA 
GCAGGGACCT ATGATGCATT GGATAGAAAC GAGGAGCTGG AGTTCCAACG CAACAAAGAA 
AGGTTTGCTT TTCTTAAATG GGCATCCAAT GCTTTCCACA ACATGCAGGT TTTCCCTCCT 
GGTTCGGGCA CTGTACACCA GGTAAATCTT GAGTATCTTG CCCGAGTTGT CTTCAACGAA 
GATGGCATTC TTTACTTCGA CAGCGTGGTT GGCACAGACT CACACACCAC TATGATTAAT 
AGTCTTGGAG TTGCTGGGTG GGGAGTAGGC GGTATTGAAG CAGTCGTTGC AATGCTTGGC 
CAGCCAATGG GCATGGTTTT GCCTGGTGTG GTTGGATTCA AATTGAGTGG GAAGTTACGG 
GATGGTGTCA CTACTACTGA CATTGTTCTT ACCATGACCC AAATGCTAAG GAAGCACGGT 
GCTATTGGCA AATTCGTTGA ATTCTATGGT GTTGGTGTGG GCGAGCTATC TTTGCCTGCT 
AGGGCCACAA TTGCCAACAT GTCTCCAGAA TATGGAGCTA CCATGGGCTT CTTCCCTGTA 
GACCAAGTAG CATTGGACTA TCTCAAACTG ACAGGCCGAA GCGATGAAAC TGTGTCGATG 
ATTGAAGCAT ACCTGCGAGC TAACAAAATG TTTGTGGACA AGCATGAGCC TGAGACGGAA 
CGCGTTTTCT CTTCACATCT GGAGCTGGAC CTTAGTGAGG TGGAGCCTTG CGTTTCAGGC 
CCCAAAAGGC CTCATGATCG TGTCCCTTTG AAGGAAATGA AGTCAGACTG GCATGCTTGC 
CTGGACAACG AAGTTGGCTT CAAGGGCTAT GCGGTGCCAA AGGAACAGCA AGGCAAAGTT 
GTGAAATTCG ACTTCCATGG AAGGCCAGCT GAAATCAAGC ATGGCAGTGT TGTTCTTGCA 
GCAATATGTA GCTCCACAAA CACATCAAAT CCCAGCGTCA TGATTGGTGC TGGCCTTGTG 
GCAAAGAAAG CCTGCGAATT AGGCCTTGAG GTGAAGCCAT GGGTAAAGAC AAGCCTTACC 
CCTGGATCTG TGGTTGCTAC CGAGTACTTG AAACATAGTG GCCTTCAAGA TTATCTGAAC 
CAGCAAGGGT TCCATGTCGC TGCGCATGGT TGCGCCACTT GTGTGGGCAA CTCCGGTGAC 
CTGGATGGAT CTGTATCAGC TGCTATCACA GAAAATGATA TGGTTGCTGC TGCGGTGCTG 
TCGGCCAACC GAAACTTCGA GGGGCGTGTG AACCCTCTCA CTCGGGCTAA CTACCTTGCC 
TCGCCGCCTC TGGTTGTTGC CTATGCTCTT GCCGGCACTG TTGACATTGA CTTTGAGAAA 
GAGCCCATTG GAGTTGGAAA GGGTGGTAAG GAAGTTTTCT TGAGGGACAT ATGGCCTTCA 
AACCAAGAGA TCGATGAGGT TGTGGAGTCC AGCGTGCAGA CTCACCTGTT CAAGAAAGTG 
TACGACTCAA TCATGGAACG TAACCCTAGG TGGAACCAGC TGCCGGTTCC AAAGGAGGCA 
CTGTACCCAT GGGAAGACAG GTCCACCTAC ATCCGCAAGC CTACGTACCT GGAGGGCATG 
TCCATGACGC CGCCTGCAGC CCCGCCCACG GTGACGGAGG CCTACTGCCT GCTCAACCTT 
GGGGACAGCA TCACCACGGA CCACATCTCC TACTCGGGGA AGATCCCCGA GGGCACACCT 
GCGGCCAAGT ATCTGCTCGA GTATGGCGTG GAGCCCAAGA ACTTCAGCTC CTATGGTGGC 
CGCCGCGGGA ACAACGAGGT GGTGATGAGG GGAGCGTTTG CCAATATGAG GATCGTGAAT 
AAGCTTCTTG ATGGCAAGGC TGGTCCCTGG ACGATCCATG TTCCTACCGG GGAGAAGCTC 
TATGTTTACG ACGCGGCAAT GAAGTACAAG TCGGAAGGTC ATGACATGGT CATCATTGCT 
GGCTCCGAGT ACGGCAGTGG CAGCTCTCGT GACTCTGCTG CCAAGGGACC AATGCTACTG 
GGTGTCAAGT CGGTGATTGC AAAGAGCTTC GAGCGGATCC ACCGGAGCAA CTTGGTGGGG 
ATGGGGATCA TTCCTCTCTG CTTTAAAGCA GGAGAGGATG CCGATTCACT CGGCCTCACC 
GGACGTGAGC GCTACACCAT CCACCTCCCT ACCAGCACCG CCGAGCTCAG TCCAGGCCAG 
GACGTGACCG TCACAACCCA CGACGGCAGA TCCTTCACTT GCACTCTTCG CTTGGACACA 
CAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100281040 ( XM_020545035.1 ) cDNA ORF clone, Zea mays -> XP_020400624.1 Zea mays aconitase 2 (aco2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OZa56263
Clone ID Related Accession (Same CDS sequence)	XM_020545035.1 , XM_020545035.2
Accession Version	XM_020545035.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1489852800000
Organism	Zea mays
Product	aconitase 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024468.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACA CTGGAGTCCC AGCAGTTGTA GACCTTGCAG CTATGCGTGA TATGATGCCA 
AAGTTGGGAT GTGATCCCTA CAAGATTAAT CCATTGATTC CCGTGGATGC TGTTATTGAC 
CACGCAGTGC GAGTGGATGT AGCAGGGACC TATGATGCAT TGGATAGAAA CGAGGAGCTG 
GAGTTCCAAC GCAACAAAGA AAGGTTTGCT TTTCTTAAAT GGGCATCCAA TGCTTTCCAC 
AACATGCAGG TTTTCCCTCC TGGTTCGGGC ACTGTACACC AGGTAAATCT TGAGTATCTT 
GCCCGAGTTG TCTTCAACGA AGATGGCATT CTTTACTTCG ACAGCGTGGT TGGCACAGAC 
TCACACACCA CTATGATTAA TAGTCTTGGA GTTGCTGGGT GGGGAGTAGG CGGTATTGAA 
GCAGTCGTTG CAATGCTTGG CCAGCCAATG GGCATGGTTT TGCCTGGTGT GGTTGGATTC 
AAATTGAGTG GGAAGTTACG GGATGGTGTC ACTACTACTG ACATTGTTCT TACCATGACC 
CAAATGCTAA GGAAGCACGG TGCTATTGGC AAATTCGTTG AATTCTATGG TGTTGGTGTG 
GGCGAGCTAT CTTTGCCTGC TAGGGCCACA ATTGCCAACA TGTCTCCAGA ATATGGAGCT 
ACCATGGGCT TCTTCCCTGT AGACCAAGTA GCATTGGACT ATCTCAAACT GACAGGCCGA 
AGCGATGAAA CTGTGTCGAT GATTGAAGCA TACCTGCGAG CTAACAAAAT GTTTGTGGAC 
AAGCATGAGC CTGAGACGGA ACGCGTTTTC TCTTCACATC TGGAGCTGGA CCTTAGTGAG 
GTGGAGCCTT GCGTTTCAGG CCCCAAAAGG CCTCATGATC GTGTCCCTTT GAAGGAAATG 
AAGTCAGACT GGCATGCTTG CCTGGACAAC GAAGTTGGCT TCAAGGGCTA TGCGGTGCCA 
AAGGAACAGC AAGGCAAAGT TGTGAAATTC GACTTCCATG GAAGGCCAGC TGAAATCAAG 
CATGGCAGTG TTGTTCTTGC AGCAATATGT AGCTCCACAA ACACATCAAA TCCCAGCGTC 
ATGATTGGTG CTGGCCTTGT GGCAAAGAAA GCCTGCGAAT TAGGCCTTGA GGTGAAGCCA 
TGGGTAAAGA CAAGCCTTAC CCCTGGATCT GTGGTTGCTA CCGAGTACTT GAAACATAGT 
GGCCTTCAAG ATTATCTGAA CCAGCAAGGG TTCCATGTCG CTGCGCATGG TTGCGCCACT 
TGTGTGGGCA ACTCCGGTGA CCTGGATGGA TCTGTATCAG CTGCTATCAC AGAAAATGAT 
ATGGTTGCTG CTGCGGTGCT GTCGGCCAAC CGAAACTTCG AGGGGCGTGT GAACCCTCTC 
ACTCGGGCTA ACTACCTTGC CTCGCCGCCT CTGGTTGTTG CCTATGCTCT TGCCGGCACT 
GTTGACATTG ACTTTGAGAA AGAGCCCATT GGAGTTGGAA AGGGTGGTAA GGAAGTTTTC 
TTGAGGGACA TATGGCCTTC AAACCAAGAG ATCGATGAGG TTGTGGAGTC CAGCGTGCAG 
ACTCACCTGT TCAAGAAAGT GTACGACTCA ATCATGGAAC GTAACCCTAG GTGGAACCAG 
CTGCCGGTTC CAAAGGAGGC ACTGTACCCA TGGGAAGACA GGTCCACCTA CATCCGCAAG 
CCTACGTACC TGGAGGGCAT GTCCATGACG CCGCCTGCAG CCCCGCCCAC GGTGACGGAG 
GCCTACTGCC TGCTCAACCT TGGGGACAGC ATCACCACGG ACCACATCTC CTACTCGGGG 
AAGATCCCCG AGGGCACACC TGCGGCCAAG TATCTGCTCG AGTATGGCGT GGAGCCCAAG 
AACTTCAGCT CCTATGGTGG CCGCCGCGGG AACAACGAGG TGGTGATGAG GGGAGCGTTT 
GCCAATATGA GGATCGTGAA TAAGCTTCTT GATGGCAAGG CTGGTCCCTG GACGATCCAT 
GTTCCTACCG GGGAGAAGCT CTATGTTTAC GACGCGGCAA TGAAGTACAA GTCGGAAGGT 
CATGACATGG TCATCATTGC TGGCTCCGAG TACGGCAGTG GCAGCTCTCG TGACTCTGCT 
GCCAAGGGAC CAATGCTACT GGGTGTCAAG TCGGTGATTG CAAAGAGCTT CGAGCGGATC 
CACCGGAGCA ACTTGGTGGG GATGGGGATC ATTCCTCTCT GCTTTAAAGC AGGAGAGGAT 
GCCGATTCAC TCGGCCTCAC CGGACGTGAG CGCTACACCA TCCACCTCCC TACCAGCACC 
GCCGAGCTCA GTCCAGGCCA GGACGTGACC GTCACAACCC ACGACGGCAG ATCCTTCACT 
TGCACTCTTC GCTTGGACAC ACAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020400624.1
CDS	739..3108
Translation	MDNTGVPAVVDLAAMRDMMPKLGCDPYKINPLIPVDAVIDHAVRVDVAGTYDALDRNEELEFQRNKERFAFLKWASNAFHNMQVFPPGSGTVHQVNLEYLARVVFNEDGILYFDSVVGTDSHTTMINSLGVAGWGVGGIEAVVAMLGQPMGMVLPGVVGFKLSGKLRDGVTTTDIVLTMTQMLRKHGAIGKFVEFYGVGVGELSLPARATIANMSPEYGATMGFFPVDQVALDYLKLTGRSDETVSMIEAYLRANKMFVDKHEPETERVFSSHLELDLSEVEPCVSGPKRPHDRVPLKEMKSDWHACLDNEVGFKGYAVPKEQQGKVVKFDFHGRPAEIKHGSVVLAAICSSTNTSNPSVMIGAGLVAKKACELGLEVKPWVKTSLTPGSVVATEYLKHSGLQDYLNQQGFHVAAHGCATCVGNSGDLDGSVSAAITENDMVAAAVLSANRNFEGRVNPLTRANYLASPPLVVAYALAGTVDIDFEKEPIGVGKGGKEVFLRDIWPSNQEIDEVVESSVQTHLFKKVYDSIMERNPRWNQLPVPKEALYPWEDRSTYIRKPTYLEGMSMTPPAAPPTVTEAYCLLNLGDSITTDHISYSGKIPEGTPAAKYLLEYGVEPKNFSSYGGRRGNNEVVMRGAFANMRIVNKLLDGKAGPWTIHVPTGEKLYVYDAAMKYKSEGHDMVIIAGSEYGSGSSRDSAAKGPMLLGVKSVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGRERYTIHLPTSTAELSPGQDVTVTTHDGRSFTCTLRLDTQV

Target ORF information:

RefSeq Version	XM_020545035.1
Organism	Zea mays
Definition	Zea mays aconitase 2 (aco2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020545035.1

ORF Insert Sequence:

ATGGACAACA CTGGAGTCCC AGCAGTTGTA GACCTTGCAG CTATGCGTGA TATGATGCCA 
AAGTTGGGAT GTGATCCCTA CAAGATTAAT CCATTGATTC CCGTGGATGC TGTTATTGAC 
CACGCAGTGC GAGTGGATGT AGCAGGGACC TATGATGCAT TGGATAGAAA CGAGGAGCTG 
GAGTTCCAAC GCAACAAAGA AAGGTTTGCT TTTCTTAAAT GGGCATCCAA TGCTTTCCAC 
AACATGCAGG TTTTCCCTCC TGGTTCGGGC ACTGTACACC AGGTAAATCT TGAGTATCTT 
GCCCGAGTTG TCTTCAACGA AGATGGCATT CTTTACTTCG ACAGCGTGGT TGGCACAGAC 
TCACACACCA CTATGATTAA TAGTCTTGGA GTTGCTGGGT GGGGAGTAGG CGGTATTGAA 
GCAGTCGTTG CAATGCTTGG CCAGCCAATG GGCATGGTTT TGCCTGGTGT GGTTGGATTC 
AAATTGAGTG GGAAGTTACG GGATGGTGTC ACTACTACTG ACATTGTTCT TACCATGACC 
CAAATGCTAA GGAAGCACGG TGCTATTGGC AAATTCGTTG AATTCTATGG TGTTGGTGTG 
GGCGAGCTAT CTTTGCCTGC TAGGGCCACA ATTGCCAACA TGTCTCCAGA ATATGGAGCT 
ACCATGGGCT TCTTCCCTGT AGACCAAGTA GCATTGGACT ATCTCAAACT GACAGGCCGA 
AGCGATGAAA CTGTGTCGAT GATTGAAGCA TACCTGCGAG CTAACAAAAT GTTTGTGGAC 
AAGCATGAGC CTGAGACGGA ACGCGTTTTC TCTTCACATC TGGAGCTGGA CCTTAGTGAG 
GTGGAGCCTT GCGTTTCAGG CCCCAAAAGG CCTCATGATC GTGTCCCTTT GAAGGAAATG 
AAGTCAGACT GGCATGCTTG CCTGGACAAC GAAGTTGGCT TCAAGGGCTA TGCGGTGCCA 
AAGGAACAGC AAGGCAAAGT TGTGAAATTC GACTTCCATG GAAGGCCAGC TGAAATCAAG 
CATGGCAGTG TTGTTCTTGC AGCAATATGT AGCTCCACAA ACACATCAAA TCCCAGCGTC 
ATGATTGGTG CTGGCCTTGT GGCAAAGAAA GCCTGCGAAT TAGGCCTTGA GGTGAAGCCA 
TGGGTAAAGA CAAGCCTTAC CCCTGGATCT GTGGTTGCTA CCGAGTACTT GAAACATAGT 
GGCCTTCAAG ATTATCTGAA CCAGCAAGGG TTCCATGTCG CTGCGCATGG TTGCGCCACT 
TGTGTGGGCA ACTCCGGTGA CCTGGATGGA TCTGTATCAG CTGCTATCAC AGAAAATGAT 
ATGGTTGCTG CTGCGGTGCT GTCGGCCAAC CGAAACTTCG AGGGGCGTGT GAACCCTCTC 
ACTCGGGCTA ACTACCTTGC CTCGCCGCCT CTGGTTGTTG CCTATGCTCT TGCCGGCACT 
GTTGACATTG ACTTTGAGAA AGAGCCCATT GGAGTTGGAA AGGGTGGTAA GGAAGTTTTC 
TTGAGGGACA TATGGCCTTC AAACCAAGAG ATCGATGAGG TTGTGGAGTC CAGCGTGCAG 
ACTCACCTGT TCAAGAAAGT GTACGACTCA ATCATGGAAC GTAACCCTAG GTGGAACCAG 
CTGCCGGTTC CAAAGGAGGC ACTGTACCCA TGGGAAGACA GGTCCACCTA CATCCGCAAG 
CCTACGTACC TGGAGGGCAT GTCCATGACG CCGCCTGCAG CCCCGCCCAC GGTGACGGAG 
GCCTACTGCC TGCTCAACCT TGGGGACAGC ATCACCACGG ACCACATCTC CTACTCGGGG 
AAGATCCCCG AGGGCACACC TGCGGCCAAG TATCTGCTCG AGTATGGCGT GGAGCCCAAG 
AACTTCAGCT CCTATGGTGG CCGCCGCGGG AACAACGAGG TGGTGATGAG GGGAGCGTTT 
GCCAATATGA GGATCGTGAA TAAGCTTCTT GATGGCAAGG CTGGTCCCTG GACGATCCAT 
GTTCCTACCG GGGAGAAGCT CTATGTTTAC GACGCGGCAA TGAAGTACAA GTCGGAAGGT 
CATGACATGG TCATCATTGC TGGCTCCGAG TACGGCAGTG GCAGCTCTCG TGACTCTGCT 
GCCAAGGGAC CAATGCTACT GGGTGTCAAG TCGGTGATTG CAAAGAGCTT CGAGCGGATC 
CACCGGAGCA ACTTGGTGGG GATGGGGATC ATTCCTCTCT GCTTTAAAGC AGGAGAGGAT 
GCCGATTCAC TCGGCCTCAC CGGACGTGAG CGCTACACCA TCCACCTCCC TACCAGCACC 
GCCGAGCTCA GTCCAGGCCA GGACGTGACC GTCACAACCC ACGACGGCAG ATCCTTCACT 
TGCACTCTTC GCTTGGACAC ACAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100281040 ( XM_020545035.2 ) cDNA ORF clone, Zea mays -> XP_020400624.1 Zea mays aconitase 2 (aco2), transcript variant X2, mRNA.

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CloneID	OZa56263
Clone ID Related Accession (Same CDS sequence)	XM_020545035.1 , XM_020545035.2
Accession Version	XM_020545035.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	aconitase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024468.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2017 this sequence version replaced XM_020545035.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAACA CTGGAGTCCC AGCAGTTGTA GACCTTGCAG CTATGCGTGA TATGATGCCA 
AAGTTGGGAT GTGATCCCTA CAAGATTAAT CCATTGATTC CCGTGGATGC TGTTATTGAC 
CACGCAGTGC GAGTGGATGT AGCAGGGACC TATGATGCAT TGGATAGAAA CGAGGAGCTG 
GAGTTCCAAC GCAACAAAGA AAGGTTTGCT TTTCTTAAAT GGGCATCCAA TGCTTTCCAC 
AACATGCAGG TTTTCCCTCC TGGTTCGGGC ACTGTACACC AGGTAAATCT TGAGTATCTT 
GCCCGAGTTG TCTTCAACGA AGATGGCATT CTTTACTTCG ACAGCGTGGT TGGCACAGAC 
TCACACACCA CTATGATTAA TAGTCTTGGA GTTGCTGGGT GGGGAGTAGG CGGTATTGAA 
GCAGTCGTTG CAATGCTTGG CCAGCCAATG GGCATGGTTT TGCCTGGTGT GGTTGGATTC 
AAATTGAGTG GGAAGTTACG GGATGGTGTC ACTACTACTG ACATTGTTCT TACCATGACC 
CAAATGCTAA GGAAGCACGG TGCTATTGGC AAATTCGTTG AATTCTATGG TGTTGGTGTG 
GGCGAGCTAT CTTTGCCTGC TAGGGCCACA ATTGCCAACA TGTCTCCAGA ATATGGAGCT 
ACCATGGGCT TCTTCCCTGT AGACCAAGTA GCATTGGACT ATCTCAAACT GACAGGCCGA 
AGCGATGAAA CTGTGTCGAT GATTGAAGCA TACCTGCGAG CTAACAAAAT GTTTGTGGAC 
AAGCATGAGC CTGAGACGGA ACGCGTTTTC TCTTCACATC TGGAGCTGGA CCTTAGTGAG 
GTGGAGCCTT GCGTTTCAGG CCCCAAAAGG CCTCATGATC GTGTCCCTTT GAAGGAAATG 
AAGTCAGACT GGCATGCTTG CCTGGACAAC GAAGTTGGCT TCAAGGGCTA TGCGGTGCCA 
AAGGAACAGC AAGGCAAAGT TGTGAAATTC GACTTCCATG GAAGGCCAGC TGAAATCAAG 
CATGGCAGTG TTGTTCTTGC AGCAATATGT AGCTCCACAA ACACATCAAA TCCCAGCGTC 
ATGATTGGTG CTGGCCTTGT GGCAAAGAAA GCCTGCGAAT TAGGCCTTGA GGTGAAGCCA 
TGGGTAAAGA CAAGCCTTAC CCCTGGATCT GTGGTTGCTA CCGAGTACTT GAAACATAGT 
GGCCTTCAAG ATTATCTGAA CCAGCAAGGG TTCCATGTCG CTGCGCATGG TTGCGCCACT 
TGTGTGGGCA ACTCCGGTGA CCTGGATGGA TCTGTATCAG CTGCTATCAC AGAAAATGAT 
ATGGTTGCTG CTGCGGTGCT GTCGGCCAAC CGAAACTTCG AGGGGCGTGT GAACCCTCTC 
ACTCGGGCTA ACTACCTTGC CTCGCCGCCT CTGGTTGTTG CCTATGCTCT TGCCGGCACT 
GTTGACATTG ACTTTGAGAA AGAGCCCATT GGAGTTGGAA AGGGTGGTAA GGAAGTTTTC 
TTGAGGGACA TATGGCCTTC AAACCAAGAG ATCGATGAGG TTGTGGAGTC CAGCGTGCAG 
ACTCACCTGT TCAAGAAAGT GTACGACTCA ATCATGGAAC GTAACCCTAG GTGGAACCAG 
CTGCCGGTTC CAAAGGAGGC ACTGTACCCA TGGGAAGACA GGTCCACCTA CATCCGCAAG 
CCTACGTACC TGGAGGGCAT GTCCATGACG CCGCCTGCAG CCCCGCCCAC GGTGACGGAG 
GCCTACTGCC TGCTCAACCT TGGGGACAGC ATCACCACGG ACCACATCTC CTACTCGGGG 
AAGATCCCCG AGGGCACACC TGCGGCCAAG TATCTGCTCG AGTATGGCGT GGAGCCCAAG 
AACTTCAGCT CCTATGGTGG CCGCCGCGGG AACAACGAGG TGGTGATGAG GGGAGCGTTT 
GCCAATATGA GGATCGTGAA TAAGCTTCTT GATGGCAAGG CTGGTCCCTG GACGATCCAT 
GTTCCTACCG GGGAGAAGCT CTATGTTTAC GACGCGGCAA TGAAGTACAA GTCGGAAGGT 
CATGACATGG TCATCATTGC TGGCTCCGAG TACGGCAGTG GCAGCTCTCG TGACTCTGCT 
GCCAAGGGAC CAATGCTACT GGGTGTCAAG TCGGTGATTG CAAAGAGCTT CGAGCGGATC 
CACCGGAGCA ACTTGGTGGG GATGGGGATC ATTCCTCTCT GCTTTAAAGC AGGAGAGGAT 
GCCGATTCAC TCGGCCTCAC CGGACGTGAG CGCTACACCA TCCACCTCCC TACCAGCACC 
GCCGAGCTCA GTCCAGGCCA GGACGTGACC GTCACAACCC ACGACGGCAG ATCCTTCACT 
TGCACTCTTC GCTTGGACAC ACAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020400624.1
CDS	738..3107
Translation	MDNTGVPAVVDLAAMRDMMPKLGCDPYKINPLIPVDAVIDHAVRVDVAGTYDALDRNEELEFQRNKERFAFLKWASNAFHNMQVFPPGSGTVHQVNLEYLARVVFNEDGILYFDSVVGTDSHTTMINSLGVAGWGVGGIEAVVAMLGQPMGMVLPGVVGFKLSGKLRDGVTTTDIVLTMTQMLRKHGAIGKFVEFYGVGVGELSLPARATIANMSPEYGATMGFFPVDQVALDYLKLTGRSDETVSMIEAYLRANKMFVDKHEPETERVFSSHLELDLSEVEPCVSGPKRPHDRVPLKEMKSDWHACLDNEVGFKGYAVPKEQQGKVVKFDFHGRPAEIKHGSVVLAAICSSTNTSNPSVMIGAGLVAKKACELGLEVKPWVKTSLTPGSVVATEYLKHSGLQDYLNQQGFHVAAHGCATCVGNSGDLDGSVSAAITENDMVAAAVLSANRNFEGRVNPLTRANYLASPPLVVAYALAGTVDIDFEKEPIGVGKGGKEVFLRDIWPSNQEIDEVVESSVQTHLFKKVYDSIMERNPRWNQLPVPKEALYPWEDRSTYIRKPTYLEGMSMTPPAAPPTVTEAYCLLNLGDSITTDHISYSGKIPEGTPAAKYLLEYGVEPKNFSSYGGRRGNNEVVMRGAFANMRIVNKLLDGKAGPWTIHVPTGEKLYVYDAAMKYKSEGHDMVIIAGSEYGSGSSRDSAAKGPMLLGVKSVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGRERYTIHLPTSTAELSPGQDVTVTTHDGRSFTCTLRLDTQV

Target ORF information:

RefSeq Version	XM_020545035.2
Organism	Zea mays
Definition	Zea mays aconitase 2 (aco2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020545035.2

ORF Insert Sequence:

ATGGACAACA CTGGAGTCCC AGCAGTTGTA GACCTTGCAG CTATGCGTGA TATGATGCCA 
AAGTTGGGAT GTGATCCCTA CAAGATTAAT CCATTGATTC CCGTGGATGC TGTTATTGAC 
CACGCAGTGC GAGTGGATGT AGCAGGGACC TATGATGCAT TGGATAGAAA CGAGGAGCTG 
GAGTTCCAAC GCAACAAAGA AAGGTTTGCT TTTCTTAAAT GGGCATCCAA TGCTTTCCAC 
AACATGCAGG TTTTCCCTCC TGGTTCGGGC ACTGTACACC AGGTAAATCT TGAGTATCTT 
GCCCGAGTTG TCTTCAACGA AGATGGCATT CTTTACTTCG ACAGCGTGGT TGGCACAGAC 
TCACACACCA CTATGATTAA TAGTCTTGGA GTTGCTGGGT GGGGAGTAGG CGGTATTGAA 
GCAGTCGTTG CAATGCTTGG CCAGCCAATG GGCATGGTTT TGCCTGGTGT GGTTGGATTC 
AAATTGAGTG GGAAGTTACG GGATGGTGTC ACTACTACTG ACATTGTTCT TACCATGACC 
CAAATGCTAA GGAAGCACGG TGCTATTGGC AAATTCGTTG AATTCTATGG TGTTGGTGTG 
GGCGAGCTAT CTTTGCCTGC TAGGGCCACA ATTGCCAACA TGTCTCCAGA ATATGGAGCT 
ACCATGGGCT TCTTCCCTGT AGACCAAGTA GCATTGGACT ATCTCAAACT GACAGGCCGA 
AGCGATGAAA CTGTGTCGAT GATTGAAGCA TACCTGCGAG CTAACAAAAT GTTTGTGGAC 
AAGCATGAGC CTGAGACGGA ACGCGTTTTC TCTTCACATC TGGAGCTGGA CCTTAGTGAG 
GTGGAGCCTT GCGTTTCAGG CCCCAAAAGG CCTCATGATC GTGTCCCTTT GAAGGAAATG 
AAGTCAGACT GGCATGCTTG CCTGGACAAC GAAGTTGGCT TCAAGGGCTA TGCGGTGCCA 
AAGGAACAGC AAGGCAAAGT TGTGAAATTC GACTTCCATG GAAGGCCAGC TGAAATCAAG 
CATGGCAGTG TTGTTCTTGC AGCAATATGT AGCTCCACAA ACACATCAAA TCCCAGCGTC 
ATGATTGGTG CTGGCCTTGT GGCAAAGAAA GCCTGCGAAT TAGGCCTTGA GGTGAAGCCA 
TGGGTAAAGA CAAGCCTTAC CCCTGGATCT GTGGTTGCTA CCGAGTACTT GAAACATAGT 
GGCCTTCAAG ATTATCTGAA CCAGCAAGGG TTCCATGTCG CTGCGCATGG TTGCGCCACT 
TGTGTGGGCA ACTCCGGTGA CCTGGATGGA TCTGTATCAG CTGCTATCAC AGAAAATGAT 
ATGGTTGCTG CTGCGGTGCT GTCGGCCAAC CGAAACTTCG AGGGGCGTGT GAACCCTCTC 
ACTCGGGCTA ACTACCTTGC CTCGCCGCCT CTGGTTGTTG CCTATGCTCT TGCCGGCACT 
GTTGACATTG ACTTTGAGAA AGAGCCCATT GGAGTTGGAA AGGGTGGTAA GGAAGTTTTC 
TTGAGGGACA TATGGCCTTC AAACCAAGAG ATCGATGAGG TTGTGGAGTC CAGCGTGCAG 
ACTCACCTGT TCAAGAAAGT GTACGACTCA ATCATGGAAC GTAACCCTAG GTGGAACCAG 
CTGCCGGTTC CAAAGGAGGC ACTGTACCCA TGGGAAGACA GGTCCACCTA CATCCGCAAG 
CCTACGTACC TGGAGGGCAT GTCCATGACG CCGCCTGCAG CCCCGCCCAC GGTGACGGAG 
GCCTACTGCC TGCTCAACCT TGGGGACAGC ATCACCACGG ACCACATCTC CTACTCGGGG 
AAGATCCCCG AGGGCACACC TGCGGCCAAG TATCTGCTCG AGTATGGCGT GGAGCCCAAG 
AACTTCAGCT CCTATGGTGG CCGCCGCGGG AACAACGAGG TGGTGATGAG GGGAGCGTTT 
GCCAATATGA GGATCGTGAA TAAGCTTCTT GATGGCAAGG CTGGTCCCTG GACGATCCAT 
GTTCCTACCG GGGAGAAGCT CTATGTTTAC GACGCGGCAA TGAAGTACAA GTCGGAAGGT 
CATGACATGG TCATCATTGC TGGCTCCGAG TACGGCAGTG GCAGCTCTCG TGACTCTGCT 
GCCAAGGGAC CAATGCTACT GGGTGTCAAG TCGGTGATTG CAAAGAGCTT CGAGCGGATC 
CACCGGAGCA ACTTGGTGGG GATGGGGATC ATTCCTCTCT GCTTTAAAGC AGGAGAGGAT 
GCCGATTCAC TCGGCCTCAC CGGACGTGAG CGCTACACCA TCCACCTCCC TACCAGCACC 
GCCGAGCTCA GTCCAGGCCA GGACGTGACC GTCACAACCC ACGACGGCAG ATCCTTCACT 
TGCACTCTTC GCTTGGACAC ACAGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The B73 maize genome: complexity, diversity, and dynamics.
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)
Schnable PS,Ware D,Fulton RS,Stein JC,Wei F,Pasternak S,Liang C,Zhang J,Fulton L,Graves TA,Minx P,Reily AD,Courtney L,Kruchowski SS,Tomlinson C,Strong C,Delehaunty K,Fronick C,Courtney B,Rock SM,Belter E,Du F,Kim K,Abbott RM,Cotton M,Levy A,Marchetto P,Ochoa K,Jackson SM,Gillam B,Chen W,Yan L,Higginbotham J,Cardenas M,Waligorski J,Applebaum E,Phelps L,Falcone J,Kanchi K,Thane T,Scimone A,Thane N,Henke J,Wang T,Ruppert J,Shah N,Rotter K,Hodges J,Ingenthron E,Cordes M,Kohlberg S,Sgro J,Delgado B,Mead K,Chinwalla A,Leonard S,Crouse K,Collura K,Kudrna D,Currie J,He R,Angelova A,Rajasekar S,Mueller T,Lomeli R,Scara G,Ko A,Delaney K,Wissotski M,Lopez G,Campos D,Braidotti M,Ashley E,Golser W,Kim H,Lee S,Lin J,Dujmic Z,Kim W,Talag J,Zuccolo A,Fan C,Sebastian A,Kramer M,Spiegel L,Nascimento L,Zutavern T,Miller B,Ambroise C,Muller S,Spooner W,Narechania A,Ren L,Wei S,Kumari S,Faga B,Levy MJ,McMahan L,Van Buren P,Vaughn MW,Ying K,Yeh CT,Emrich SJ,Jia Y,Kalyanaraman A,Hsia AP,Barbazuk WB,Baucom RS,Brutnell TP,Carpita NC,Chaparro C,Chia JM,Deragon JM,Estill JC,Fu Y,Jeddeloh JA,Han Y,Lee H,Li P,Lisch DR,Liu S,Liu Z,Nagel DH,McCann MC,SanMiguel P,Myers AM,Nettleton D,Nguyen J,Penning BW,Ponnala L,Schneider KL,Schwartz DC,Sharma A,Soderlund C,Springer NM,Sun Q,Wang H,Waterman M,Westerman R,Wolfgruber TK,Yang L,Yu Y,Zhang L,Zhou S,Zhu Q,Bennetzen JL,Dawe RK,Jiang J,Jiang N,Presting GG,Wessler SR,Aluru S,Martienssen RA,Clifton SW,McCombie WR,Wing RA,Wilson RK

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LOC100281040 cDNA ORF clone, Zea mays

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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