LOC100125646 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100125646
Entrez Gene ID	100125646
Full Name	catechol-O-methyltransferase
Synonyms	CAOMT,GRMZM5G814904,OMT
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
NM_001112577.2	NP_001106047.1	caffeic acid 3-O-methyltransferase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Extensive genetic diversity and low linkage disequilibrium within the COMT locus in maize exotic populations.
Chen Y, Blanco M, Ji Q, Frei UK, Lübberstedt T
Plant science : an international journal of experimental plant biology221-22269-80(2014 May)

The B73 maize genome: complexity, diversity, and dynamics.
Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, Liang C, Zhang J, Fulton L, Graves TA, Minx P, Reily AD, Courtney L, Kruchowski SS, Tomlinson C, Strong C, Delehaunty K, Fronick C, Courtney B, Rock SM, Belter E, Du F, Kim K, Abbott RM, Cotton M, Levy A, Marchetto P, Ochoa K, Jackson SM, Gillam B, Chen W, Yan L, Higginbotham J, Cardenas M, Waligorski J, Applebaum E, Phelps L, Falcone J, Kanchi K, Thane T, Scimone A, Thane N, Henke J, Wang T, Ruppert J, Shah N, Rotter K, Hodges J, Ingenthron E, Cordes M, Kohlberg S, Sgro J, Delgado B, Mead K, Chinwalla A, Leonard S, Crouse K, Collura K, Kudrna D, Currie J, He R, Angelova A, Rajasekar S, Mueller T, Lomeli R, Scara G, Ko A, Delaney K, Wissotski M, Lopez G, Campos D, Braidotti M, Ashley E, Golser W, Kim H, Lee S, Lin J, Dujmic Z, Kim W, Talag J, Zuccolo A, Fan C, Sebastian A, Kramer M, Spiegel L, Nascimento L, Zutavern T, Miller B, Ambroise C, Muller S, Spooner W, Narechania A, Ren L, Wei S, Kumari S, Faga B, Levy MJ, McMahan L, Van Buren P, Vaughn MW, Ying K, Yeh CT, Emrich SJ, Jia Y, Kalyanaraman A, Hsia AP, Barbazuk WB, Baucom RS, Brutnell TP, Carpita NC, Chaparro C, Chia JM, Deragon JM, Estill JC, Fu Y, Jeddeloh JA, Han Y, Lee H, Li P, Lisch DR, Liu S, Liu Z, Nagel DH, McCann MC, SanMiguel P, Myers AM, Nettleton D, Nguyen J, Penning BW, Ponnala L, Schneider KL, Schwartz DC, Sharma A, Soderlund C, Springer NM, Sun Q, Wang H, Waterman M, Westerman R, Wolfgruber TK, Yang L, Yu Y, Zhang L, Zhou S, Zhu Q, Bennetzen JL, Dawe RK, Jiang J, Jiang N, Presting GG, Wessler SR, Aluru S, Martienssen RA, Clifton SW, McCombie WR, Wing RA, Wilson RK
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)

Expression of cell wall related genes in basal and ear internodes of silking brown-midrib-3, caffeic acid O-methyltransferase (COMT) down-regulated, and normal maize plants.
Guillaumie S, Goffner D, Barbier O, Martinant JP, Pichon M, Barrière Y
BMC plant biology871(2008 Jun)

Genetic diversity associated with variation in silage corn digestibility for three O-methyltransferase genes involved in lignin biosynthesis.
Guillet-Claude C, Birolleau-Touchard C, Manicacci D, Fourmann M, Barraud S, Carret V, Martinant JP, Barrière Y
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik110(1)126-35(2004 Dec)

The brown midrib3 (bm3) mutation in maize occurs in the gene encoding caffeic acid O-methyltransferase.
Vignols F, Rigau J, Torres MA, Capellades M, Puigdomènech P
The Plant cell7(4)407-16(1995 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC100125646 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100125646 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OZa67356	NM_001112577.2 Latest version!	Zea mays catechol-O-methyltransferase (LOC100125646), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100125646 ( NM_001112577.2 ) cDNA ORF clone, Zea mays -> NP_001106047.1 Zea mays catechol-O-methyltransferase (LOC100125646), mRNA.

Price & Availability↑

CloneID	OZa67356
Clone ID Related Accession (Same CDS sequence)	NM_001112577.2
Accession Version	NM_001112577.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577462400000
Organism	Zea mays
Product	caffeic acid 3-O-methyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EU965480.1, BM382769.1 and EU941801.1. On Dec 17, 2016 this sequence version replaced NM_001112577.1. ##Evidence-Data-START## Transcript exon combination :: EU965480.1, SRR3147046.165.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00780084, SAMN00780085 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTCCA CCGCCGGCGA CGTGGCCGCG GTGGTGGACG AGGAGGCGTG CATGTACGCG 
ATGCAGCTGG CGTCGTCGTC CATCCTGCCC ATGACGCTGA AGAACGCCAT CGAGCTGGGC 
CTGCTGGAGG TGCTGCAGAA GGAGGCCGGC GGCGGCAAGG CGGCGCTGGC GCCCGAGGAG 
GTGGTGGCGC GGATGCCCGC GGCGCCCGGC GACCCCGCCG CCGCGGCGGC CATGGTGGAC 
CGCATGCTCC GCCTGCTCGC CTCCTACGAC GTCGTCCGGT GCCAGATGGA GGACCGGGAC 
GGCCGGTACG AGCGCCGCTA CTCCGCCGCG CCCGTCTGCA AGTGGCTCAC CCCCAACGAG 
GACGGCGTGT CCATGGCCGC CCTCGCGCTC ATGAACCAGG ACAAGGTCCT CATGGAGAGC 
TGGTACTATC TCAAGGACGC GGTGCTGGAC GGCGGCATCC CGTTCAACAA GGCGTACGGG 
ATGACGGCGT TCGAGTACCA CGGCACGGAC TCGCGCTTCA ACCGCGTGTT CAACGAGGGC 
ATGAAGAACC ACTCGGTGAT CATCACCAAG AAGCTGCTGG ACTTCTACAC GGGCTTCGAG 
GGCGTGTCGA CGCTGGTGGA CGTGGGCGGC GGCGTGGGCG CCACGCTGCA CGCCATCACG 
TCCCGCCACC CGCACATCTC CGGGGTCAAC TTCGACCTGC CGCACGTCAT CTCCGAGGCG 
CCGCCGTTCC CCGGCGTGCG CCACGTGGGC GGGGACATGT TCGCGTCCGT GCCCGCCGGC 
GACGCCATCC TCATGAAGTG GATCCTCCAC GACTGGAGCG ACGCGCACTG CGCCACGCTG 
CTCAAGAACT GCTACGACGC GCTGCCGGAA AATGGCAAGG TCATCGTCGT CGAGTGCGTG 
CTGCCGGTCA ACACGGAGGC CACCCCCAAG GCGCAGGGCG TCTTCCACGT CGACATGATC 
ATGCTCGCGC ACAACCCCGG CGGCAAGGAG CGGTACGAGC GCGAGTTCCG CGAGCTCGCA 
AAGGGCGCCG GCTTCTCCGG GTTCAAGGCC ACCTACATCT ACGCCAACGC CTGGGCCATC 
GAGTTCATCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106047.1
CDS	120..1214
Translation	MGSTAGDVAAVVDEEACMYAMQLASSSILPMTLKNAIELGLLEVLQKEAGGGKAALAPEEVVARMPAAPGDPAAAAAMVDRMLRLLASYDVVRCQMEDRDGRYERRYSAAPVCKWLTPNEDGVSMAALALMNQDKVLMESWYYLKDAVLDGGIPFNKAYGMTAFEYHGTDSRFNRVFNEGMKNHSVIITKKLLDFYTGFEGVSTLVDVGGGVGATLHAITSRHPHISGVNFDLPHVISEAPPFPGVRHVGGDMFASVPAGDAILMKWILHDWSDAHCATLLKNCYDALPENGKVIVVECVLPVNTEATPKAQGVFHVDMIMLAHNPGGKERYEREFRELAKGAGFSGFKATYIYANAWAIEFIK

Target ORF information:

RefSeq Version	NM_001112577.2
Organism	Zea mays
Definition	Zea mays catechol-O-methyltransferase (LOC100125646), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112577.2

ORF Insert Sequence:

ATGGGCTCCA CCGCCGGCGA CGTGGCCGCG GTGGTGGACG AGGAGGCGTG CATGTACGCG 
ATGCAGCTGG CGTCGTCGTC CATCCTGCCC ATGACGCTGA AGAACGCCAT CGAGCTGGGC 
CTGCTGGAGG TGCTGCAGAA GGAGGCCGGC GGCGGCAAGG CGGCGCTGGC GCCCGAGGAG 
GTGGTGGCGC GGATGCCCGC GGCGCCCGGC GACCCCGCCG CCGCGGCGGC CATGGTGGAC 
CGCATGCTCC GCCTGCTCGC CTCCTACGAC GTCGTCCGGT GCCAGATGGA GGACCGGGAC 
GGCCGGTACG AGCGCCGCTA CTCCGCCGCG CCCGTCTGCA AGTGGCTCAC CCCCAACGAG 
GACGGCGTGT CCATGGCCGC CCTCGCGCTC ATGAACCAGG ACAAGGTCCT CATGGAGAGC 
TGGTACTATC TCAAGGACGC GGTGCTGGAC GGCGGCATCC CGTTCAACAA GGCGTACGGG 
ATGACGGCGT TCGAGTACCA CGGCACGGAC TCGCGCTTCA ACCGCGTGTT CAACGAGGGC 
ATGAAGAACC ACTCGGTGAT CATCACCAAG AAGCTGCTGG ACTTCTACAC GGGCTTCGAG 
GGCGTGTCGA CGCTGGTGGA CGTGGGCGGC GGCGTGGGCG CCACGCTGCA CGCCATCACG 
TCCCGCCACC CGCACATCTC CGGGGTCAAC TTCGACCTGC CGCACGTCAT CTCCGAGGCG 
CCGCCGTTCC CCGGCGTGCG CCACGTGGGC GGGGACATGT TCGCGTCCGT GCCCGCCGGC 
GACGCCATCC TCATGAAGTG GATCCTCCAC GACTGGAGCG ACGCGCACTG CGCCACGCTG 
CTCAAGAACT GCTACGACGC GCTGCCGGAA AATGGCAAGG TCATCGTCGT CGAGTGCGTG 
CTGCCGGTCA ACACGGAGGC CACCCCCAAG GCGCAGGGCG TCTTCCACGT CGACATGATC 
ATGCTCGCGC ACAACCCCGG CGGCAAGGAG CGGTACGAGC GCGAGTTCCG CGAGCTCGCA 
AAGGGCGCCG GCTTCTCCGG GTTCAAGGCC ACCTACATCT ACGCCAACGC CTGGGCCATC 
GAGTTCATCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extensive genetic diversity and low linkage disequilibrium within the COMT locus in maize exotic populations.
Plant science : an international journal of experimental plant biology221-22269-80(2014 May)
Chen Y,Blanco M,Ji Q,Frei UK,Lübberstedt T

The B73 maize genome: complexity, diversity, and dynamics.
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)
Schnable PS,Ware D,Fulton RS,Stein JC,Wei F,Pasternak S,Liang C,Zhang J,Fulton L,Graves TA,Minx P,Reily AD,Courtney L,Kruchowski SS,Tomlinson C,Strong C,Delehaunty K,Fronick C,Courtney B,Rock SM,Belter E,Du F,Kim K,Abbott RM,Cotton M,Levy A,Marchetto P,Ochoa K,Jackson SM,Gillam B,Chen W,Yan L,Higginbotham J,Cardenas M,Waligorski J,Applebaum E,Phelps L,Falcone J,Kanchi K,Thane T,Scimone A,Thane N,Henke J,Wang T,Ruppert J,Shah N,Rotter K,Hodges J,Ingenthron E,Cordes M,Kohlberg S,Sgro J,Delgado B,Mead K,Chinwalla A,Leonard S,Crouse K,Collura K,Kudrna D,Currie J,He R,Angelova A,Rajasekar S,Mueller T,Lomeli R,Scara G,Ko A,Delaney K,Wissotski M,Lopez G,Campos D,Braidotti M,Ashley E,Golser W,Kim H,Lee S,Lin J,Dujmic Z,Kim W,Talag J,Zuccolo A,Fan C,Sebastian A,Kramer M,Spiegel L,Nascimento L,Zutavern T,Miller B,Ambroise C,Muller S,Spooner W,Narechania A,Ren L,Wei S,Kumari S,Faga B,Levy MJ,McMahan L,Van Buren P,Vaughn MW,Ying K,Yeh CT,Emrich SJ,Jia Y,Kalyanaraman A,Hsia AP,Barbazuk WB,Baucom RS,Brutnell TP,Carpita NC,Chaparro C,Chia JM,Deragon JM,Estill JC,Fu Y,Jeddeloh JA,Han Y,Lee H,Li P,Lisch DR,Liu S,Liu Z,Nagel DH,McCann MC,SanMiguel P,Myers AM,Nettleton D,Nguyen J,Penning BW,Ponnala L,Schneider KL,Schwartz DC,Sharma A,Soderlund C,Springer NM,Sun Q,Wang H,Waterman M,Westerman R,Wolfgruber TK,Yang L,Yu Y,Zhang L,Zhou S,Zhu Q,Bennetzen JL,Dawe RK,Jiang J,Jiang N,Presting GG,Wessler SR,Aluru S,Martienssen RA,Clifton SW,McCombie WR,Wing RA,Wilson RK

Expression of cell wall related genes in basal and ear internodes of silking brown-midrib-3, caffeic acid O-methyltransferase (COMT) down-regulated, and normal maize plants.
BMC plant biology871(2008 Jun)
Guillaumie S,Goffner D,Barbier O,Martinant JP,Pichon M,Barrière Y

Genetic diversity associated with variation in silage corn digestibility for three O-methyltransferase genes involved in lignin biosynthesis.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik110(1)126-35(2004 Dec)
Guillet-Claude C,Birolleau-Touchard C,Manicacci D,Fourmann M,Barraud S,Carret V,Martinant JP,Barrière Y

The brown midrib3 (bm3) mutation in maize occurs in the gene encoding caffeic acid O-methyltransferase.
The Plant cell7(4)407-16(1995 Apr)
Vignols F,Rigau J,Torres MA,Capellades M,Puigdomènech P

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LOC100125646 cDNA ORF clone, Zea mays

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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