ciita cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ciita
Entrez Gene ID	101734303
Full Name	class II, major histocompatibility complex, transactivator
General protein information	Preferred Names class II, major histocompatibility complex, transactivator Names MHC class II transactivator
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018097270.2	XP_017952759.2	MHC class II transactivator
XM_018097270.1	XP_017952759.1	MHC class II transactivator

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CIITA	XP_002698008.2
Macaca mulatta (Rhesus monkey)	CIITA	XP_001103518.2
Canis lupus familiaris (dog)	CIITA	XP_005621604.1
Gallus gallus (chicken)	LOC416633	XP_004945372.1
Xenopus tropicalis (tropical clawed frog)	ciita	XP_004918329.1
Pan troglodytes (chimpanzee)	CIITA	XP_510813.2
Rattus norvegicus (Norway rat)	Ciita	NP_445981.2
Homo sapiens (human)	CIITA	NP_001273331.1
Mus musculus (house mouse)	Ciita	NP_001230689.1
Danio rerio (zebrafish)	ciita	XP_005163972.1

Gene Ontology

The following ciita gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ciita cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa46712	XM_018097270.1 Latest version!	Xenopus tropicalis class II major histocompatibility complex transactivator (ciita), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa67195	XM_018097270.2 Latest version!	Xenopus tropicalis class II, major histocompatibility complex, transactivator (ciita), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ciita ( XM_018097270.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952759.1 Xenopus tropicalis class II major histocompatibility complex transactivator (ciita), mRNA.

Price & Availability↑

CloneID	OXa46712
Clone ID Related Accession (Same CDS sequence)	XM_018097270.1
Accession Version	XM_018097270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MHC class II transactivator
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases ##RefSeq-Attributes-END##

ATGGTATTTC GGTTTATAGC ACCTTCTTCA GTGTCCCCGC CATTCCAAGC ACAACCTGCG 
CCTCCAGTTT CCACAGTTAC CATTTCAGAA ATTCCAACAG TTCCTATGAG CATCCCATGT 
TCCCCCGACA AAAACTGCTC CTTTAGTCCA TTCACTCCTG CAGCAGCTGA TTTACCAAAT 
TCCCCAGAAG CAACTGCCAT GAGTCAGTAC CCTCTGAAAG AAAATGAAAC TGTTACCCCA 
CAGTCACTAG CACCTATGGA GAATTCATTT GCAGAGAAAG AAAAGAATAA ATATGTGGAC 
TTGTTTAAAG CTGCATTGAC AGAGAAGTAC AAAAACTTAT GGGCCTTTGA AAGAGATGTA 
TTGTACACTG ACATTGCACT TGTGAAAAGC AGGATTGACC CCAAACCTGG GAAAGTTACA 
TGTAAAACAG CAGAGAAGGA ACTTGTCATT TATGATTTAG ATGAACGGGA AAAAGAAGCA 
GTGGATCATT TCAGTCTTTT TGCAGATAAA GAAAGGAAAG AGATGGAGAC AAATATAATT 
GCACTACTGG GACAATCAGG AATGGGGAAA ACTGTTCTTG CTAAAAAAAT ATGCCAAGAC 
TGGTCCAATG GCGCTTATTC ACAGTTCAGT CTAGTGATTT TTTTTGAGTG GAGCATGCTT 
GCTTCTTGTA AAAAGCAATA CAGCTTGAAA GATTTCCTTT TTGAATTGTC TTGCTGCCCA 
CATGAAAGAA ATGTTGAAAT CTACCAGTAT ATACTGAGAA ACCCAGAGAA AGTGCTGCTT 
ATCTTTGATG GTGTTGATGA GTTTCAAGAC TCAGAGGGTC TGATGCAGGC TTCTTACACC 
GCATCACCTA CAAAGACACA TAAAATAAAG GAATTATTTA CTGGCCTTTT CCAAAAGAAG 
CTGCTACGGG GGTGCACCAT GCTAATAACT GCAAGACCAA AAGATAAATT TAATCAGTAC 
TTAACTAAGG TAGACCAGAT TGTGGAAATG TCTGGATTTT CTCAGCAGCA GGTGGAATGG 
TATGTCAATG AATATTTTAA GGGGTTTCAA GATTTTACTG ACGTGCTAAA ATGGATGAGA 
GAATATGAAT ACTTATTCAG ATATTGTTAT ATCCCCTTTA TGTGTAGGAT AATGTGTCTG 
TTTGCTGAAA CTAATTTTAG GGATGAGAGA GTGGAATCTC ATTTTTCTTT AGCAGAATTT 
ATGTTGACTG TGCTTCAGAG AAACGAAGAG TTTACAGGCC AAGGTTCCTC AGGTTTGTCA 
TCTGATGAGC ATTCCAATAT GAAAGGTGAT CAAGCTGTAC AATGTGCCTC AACCCCTCAG 
CCTTCTTTAA TGGAAAATGA GATGGATTTA TCTAATGGAG TTCATACTAA ACACAAACTT 
CCAGTAAAAA AACACCATAT AAACAACGGG AATTCACTGG TTCAGCATTT TCGAACAGCT 
CAAAGTCTTA TGGAACATAT AACAGACAAG CATTTTGTCA GATATGCTTC ACTGGAGCTC 
AAAAAGAAAC GAAATCATGA TAATTGCACT GATATGGTAC GGAGGTGCTT GGTTGGACTT 
TTATTTCGTA AAGATTTAAA TACTGATCTG CTGAAAGTCA TTCTCAAAAA ACAAAAGAAA 
ATGACTGATT ACTTCAAGGC AATACAGCCT GAAACACTTT GTCCACAAAG ACTACTTGAA 
CTTTGTCACT GTGTCTGTGA GACAAATGAC ATTAGTCTAA GTCAACACAC GGCTCAAAAA 
CTTAGTAATG AACTTTCATT TGTGGGTACC AGACTTACGC CCCCAGATGT TTATGTGTTA 
AAATGCATTT TGAAGCATTC ACACACAAAA ATATCCTTGG ACCTAAGGAG AACAGGAATT 
GATCAACAAG GATTAAAGGA ATTGGTAAAA CTAAAAACCA TCAAGTCATT CAGGGCATCT 
CTCAGTGACA CGGTCAGACT CTGGAAGACC TTACAAACTG AGAACAACCT CAGACTGTTA 
AAGCTGTGTG TTATGAAATT CAGAATCGAT CCATTCACAG CAGAAAGCAT AAAAGATATA 
ACTGATCTTA CAGCCATTGT CAGTATTCAG AATGAAATTT GCAGTTGCCT TCAAGAATTA 
ACAGATGGGA TCTCTGAAAT TCCAGCTATC AAAACACTTA AAAAGATCAT ATTTAGACTT 
GGAAAGAGTC ATGGACAAAA TGGGTTCATG AGGCTTGTTG ATATCCTTCC ACAGCTTCCA 
TCTCTCCAAC ATTTAGATTT ACATTCTCTT ATTGAGAATC ACATCGGTGA TAAATCTGTT 
GCAAAGCTGG CCGAGAAATT CCCAGAAATG CTGTCTCTTG AGACACTGGA CTTATCACAA 
AACACCATAA CCGATGTCGG TGCAGCAAAG CTGGCCATGG CTCTTCCTTC CTTGGGCTCG 
CTACATACAC TTAGTTTATT CAACAACTTT ATCCAGGATG ATGGAGCCAA GAAATTTGCT 
GAGATACTTC CTGAAATGTC TTCCCTGGAA AAGCTACATC TAGCATACAA CAAGATCAGT 
GATGTTGGTG CCAGGAATCT CACAGAGAGT CTTAGGAAAT GTCCCAAAAT GAAGAGTTTG 
ATGATATACA GTTCCGCAAT TCCTCATAAT ATTTTGCATC ATCTCCAACA GCAGGACCCA 
AGGATCAGTT GCCAGTCGTT TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952759.1
CDS	1..2667
Translation	MVFRFIAPSSVSPPFQAQPAPPVSTVTISEIPTVPMSIPCSPDKNCSFSPFTPAAADLPNSPEATAMSQYPLKENETVTPQSLAPMENSFAEKEKNKYVDLFKAALTEKYKNLWAFERDVLYTDIALVKSRIDPKPGKVTCKTAEKELVIYDLDEREKEAVDHFSLFADKERKEMETNIIALLGQSGMGKTVLAKKICQDWSNGAYSQFSLVIFFEWSMLASCKKQYSLKDFLFELSCCPHERNVEIYQYILRNPEKVLLIFDGVDEFQDSEGLMQASYTASPTKTHKIKELFTGLFQKKLLRGCTMLITARPKDKFNQYLTKVDQIVEMSGFSQQQVEWYVNEYFKGFQDFTDVLKWMREYEYLFRYCYIPFMCRIMCLFAETNFRDERVESHFSLAEFMLTVLQRNEEFTGQGSSGLSSDEHSNMKGDQAVQCASTPQPSLMENEMDLSNGVHTKHKLPVKKHHINNGNSLVQHFRTAQSLMEHITDKHFVRYASLELKKKRNHDNCTDMVRRCLVGLLFRKDLNTDLLKVILKKQKKMTDYFKAIQPETLCPQRLLELCHCVCETNDISLSQHTAQKLSNELSFVGTRLTPPDVYVLKCILKHSHTKISLDLRRTGIDQQGLKELVKLKTIKSFRASLSDTVRLWKTLQTENNLRLLKLCVMKFRIDPFTAESIKDITDLTAIVSIQNEICSCLQELTDGISEIPAIKTLKKIIFRLGKSHGQNGFMRLVDILPQLPSLQHLDLHSLIENHIGDKSVAKLAEKFPEMLSLETLDLSQNTITDVGAAKLAMALPSLGSLHTLSLFNNFIQDDGAKKFAEILPEMSSLEKLHLAYNKISDVGARNLTESLRKCPKMKSLMIYSSAIPHNILHHLQQQDPRISCQSFG

Target ORF information:

RefSeq Version	XM_018097270.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis class II major histocompatibility complex transactivator (ciita), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097270.1

ORF Insert Sequence:

ATGGTATTTC GGTTTATAGC ACCTTCTTCA GTGTCCCCGC CATTCCAAGC ACAACCTGCG 
CCTCCAGTTT CCACAGTTAC CATTTCAGAA ATTCCAACAG TTCCTATGAG CATCCCATGT 
TCCCCCGACA AAAACTGCTC CTTTAGTCCA TTCACTCCTG CAGCAGCTGA TTTACCAAAT 
TCCCCAGAAG CAACTGCCAT GAGTCAGTAC CCTCTGAAAG AAAATGAAAC TGTTACCCCA 
CAGTCACTAG CACCTATGGA GAATTCATTT GCAGAGAAAG AAAAGAATAA ATATGTGGAC 
TTGTTTAAAG CTGCATTGAC AGAGAAGTAC AAAAACTTAT GGGCCTTTGA AAGAGATGTA 
TTGTACACTG ACATTGCACT TGTGAAAAGC AGGATTGACC CCAAACCTGG GAAAGTTACA 
TGTAAAACAG CAGAGAAGGA ACTTGTCATT TATGATTTAG ATGAACGGGA AAAAGAAGCA 
GTGGATCATT TCAGTCTTTT TGCAGATAAA GAAAGGAAAG AGATGGAGAC AAATATAATT 
GCACTACTGG GACAATCAGG AATGGGGAAA ACTGTTCTTG CTAAAAAAAT ATGCCAAGAC 
TGGTCCAATG GCGCTTATTC ACAGTTCAGT CTAGTGATTT TTTTTGAGTG GAGCATGCTT 
GCTTCTTGTA AAAAGCAATA CAGCTTGAAA GATTTCCTTT TTGAATTGTC TTGCTGCCCA 
CATGAAAGAA ATGTTGAAAT CTACCAGTAT ATACTGAGAA ACCCAGAGAA AGTGCTGCTT 
ATCTTTGATG GTGTTGATGA GTTTCAAGAC TCAGAGGGTC TGATGCAGGC TTCTTACACC 
GCATCACCTA CAAAGACACA TAAAATAAAG GAATTATTTA CTGGCCTTTT CCAAAAGAAG 
CTGCTACGGG GGTGCACCAT GCTAATAACT GCAAGACCAA AAGATAAATT TAATCAGTAC 
TTAACTAAGG TAGACCAGAT TGTGGAAATG TCTGGATTTT CTCAGCAGCA GGTGGAATGG 
TATGTCAATG AATATTTTAA GGGGTTTCAA GATTTTACTG ACGTGCTAAA ATGGATGAGA 
GAATATGAAT ACTTATTCAG ATATTGTTAT ATCCCCTTTA TGTGTAGGAT AATGTGTCTG 
TTTGCTGAAA CTAATTTTAG GGATGAGAGA GTGGAATCTC ATTTTTCTTT AGCAGAATTT 
ATGTTGACTG TGCTTCAGAG AAACGAAGAG TTTACAGGCC AAGGTTCCTC AGGTTTGTCA 
TCTGATGAGC ATTCCAATAT GAAAGGTGAT CAAGCTGTAC AATGTGCCTC AACCCCTCAG 
CCTTCTTTAA TGGAAAATGA GATGGATTTA TCTAATGGAG TTCATACTAA ACACAAACTT 
CCAGTAAAAA AACACCATAT AAACAACGGG AATTCACTGG TTCAGCATTT TCGAACAGCT 
CAAAGTCTTA TGGAACATAT AACAGACAAG CATTTTGTCA GATATGCTTC ACTGGAGCTC 
AAAAAGAAAC GAAATCATGA TAATTGCACT GATATGGTAC GGAGGTGCTT GGTTGGACTT 
TTATTTCGTA AAGATTTAAA TACTGATCTG CTGAAAGTCA TTCTCAAAAA ACAAAAGAAA 
ATGACTGATT ACTTCAAGGC AATACAGCCT GAAACACTTT GTCCACAAAG ACTACTTGAA 
CTTTGTCACT GTGTCTGTGA GACAAATGAC ATTAGTCTAA GTCAACACAC GGCTCAAAAA 
CTTAGTAATG AACTTTCATT TGTGGGTACC AGACTTACGC CCCCAGATGT TTATGTGTTA 
AAATGCATTT TGAAGCATTC ACACACAAAA ATATCCTTGG ACCTAAGGAG AACAGGAATT 
GATCAACAAG GATTAAAGGA ATTGGTAAAA CTAAAAACCA TCAAGTCATT CAGGGCATCT 
CTCAGTGACA CGGTCAGACT CTGGAAGACC TTACAAACTG AGAACAACCT CAGACTGTTA 
AAGCTGTGTG TTATGAAATT CAGAATCGAT CCATTCACAG CAGAAAGCAT AAAAGATATA 
ACTGATCTTA CAGCCATTGT CAGTATTCAG AATGAAATTT GCAGTTGCCT TCAAGAATTA 
ACAGATGGGA TCTCTGAAAT TCCAGCTATC AAAACACTTA AAAAGATCAT ATTTAGACTT 
GGAAAGAGTC ATGGACAAAA TGGGTTCATG AGGCTTGTTG ATATCCTTCC ACAGCTTCCA 
TCTCTCCAAC ATTTAGATTT ACATTCTCTT ATTGAGAATC ACATCGGTGA TAAATCTGTT 
GCAAAGCTGG CCGAGAAATT CCCAGAAATG CTGTCTCTTG AGACACTGGA CTTATCACAA 
AACACCATAA CCGATGTCGG TGCAGCAAAG CTGGCCATGG CTCTTCCTTC CTTGGGCTCG 
CTACATACAC TTAGTTTATT CAACAACTTT ATCCAGGATG ATGGAGCCAA GAAATTTGCT 
GAGATACTTC CTGAAATGTC TTCCCTGGAA AAGCTACATC TAGCATACAA CAAGATCAGT 
GATGTTGGTG CCAGGAATCT CACAGAGAGT CTTAGGAAAT GTCCCAAAAT GAAGAGTTTG 
ATGATATACA GTTCCGCAAT TCCTCATAAT ATTTTGCATC ATCTCCAACA GCAGGACCCA 
AGGATCAGTT GCCAGTCGTT TGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ciita ( XM_018097270.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952759.2 Xenopus tropicalis class II, major histocompatibility complex, transactivator (ciita), mRNA.

Price & Availability↑

CloneID	OXa67195
Clone ID Related Accession (Same CDS sequence)	XM_018097270.2
Accession Version	XM_018097270.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	MHC class II transactivator
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018097270.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACT TTAAGGAGAT CCTGCCTTTT ACGACACAAA TTGTCTCCAA CTCCTCCTCT 
AACGACCTAA GAAAACTTCT TAGTCAGCTT GTTGAAACCA AGATTATATC CATGGAATAT 
TATGTTAGTT TGCTAAGTGA AAATGACACA GATGATCTTG CACGGAAAAT TGCTTTGATT 
GCTGTAGAAA AACCTGAAAT TCGCCATAAA TTCCCATCAC TTTTCTGTGG ACCTGAGAGG 
GAAGACTATC CACTAGAGGC ACCTGCAGTT AGTATGGATT CAAGGCAGGA GAACCTACAG 
TTACCCAGAA AGACTGTCTC TAACTCATTC TCTAATACCA TAACTCAATT TCCCAATAAT 
TCTCTGGAAA CACAGACCAC GCCACTGGAA TATTTTCTAA ATTCACTCAG TGAGAACAAT 
ATGGATAATC TTACATTGGA TATGTCCTTA AAATTTGCAG AAAGAAATGA AATACTTCAT 
ACATGTTTAC CACTTATGGA TGAACTAGAA GAAGAATATA TTTTCACTGA TGCCACTTCA 
TTTAGGGAAA ATTCAATGAT GGATCTGAGC ACTATAGATA GTAGTGTGCT GAATTGGAGT 
TTGGAATCGG AGTCAGAATT GGACTTGGAG ACACTGTCCA CTTTGGATTT ATCAGATATT 
GAAAAGTGTT TTTTAGATGA CCCTTTGGAT TTTGAACTTG AAGATGATAA CTGCAGTAAT 
TGTATTGATA ATGATGAAGA ATATGACAAG ATTGCCAGAA TATGCAATTA TATTTGTGGT 
GAAGATCATC AGATGGAAAG TTTATTGGAT GGAGAATCAG GGAATCCTGC AGAAGCCCCA 
AACAACGAAA CATTATCAAA GAAATTAAAA AGAAAAAGAA AAATTGGGCC AGTGATACCA 
AAGCGAAAGA GGATAAACCG AGAAAAACCG ACAGCCCCTC AGTGTATGGA GGCAACAGAA 
GCTCAGGATA CCCCAGACAT TCTCCCAACA TCTAAAAGTC TCAGTGCACC CGTGCACATA 
TCTTCACTAC CTGTAATCCA GGTCTTTGGT AAATTGCCAC CAGAATTCAC GCCCCAGCAT 
ATAATCAGTT TGCCTCCTGG AGCAAATATT ATTTTCTACA CAGCACCTTC TTCAGTGTCC 
CCGCCATTCC AAGCACAACC TGCGCCTCCA GTTTCCACAG TTACCATTTC AGAAATTCCA 
ACAGTTCCTA TGAGCATCCC ATGTTCCCCC GACAAAAACT GCTCCTTTAG TCCATTCACT 
CCTGCAGCAG CTGATTTACC AAATTCCCCA GAAGCAACTG CCATGAGTCA GTACCCTCTG 
AAAGAAAATG AAACTGTTAC CCCACAGTCA CTAGCACCTA TGGAGAATTC ATTTGCAGAG 
AAAGAAAAGA ATAAATATGT GGACTTGTTT AAAGCTGCAT TGACAGAGAA GTACAAAAAC 
TTATGGGCCT TTGAAAGAGA TGTATTGTAC ACTGACATTG CACTTGTGAA AAGCAGGATT 
GACCCCAAAC CTGGGAAAGT TACATGTAAA ACAGCAGAGA AGGAACTTGT CATTTATGAT 
TTAGATGAAC GGGAAAAAGA AGCAGTGGAT CATTTCAGTC TTTTTGCAGA TAAAGAAAGG 
AAAGAGATGG AGACAAATAT AATTGCACTA CTGGGACAAT CAGGAATGGG GAAAACTGTT 
CTTGCTAAAA AAATATGCCA AGACTGGTCC AATGGCGCTT ATTCACAGTT CAGTCTAGTG 
ATTTTTTTTG AGTGGAGCAT GCTTGCTTCT TGTAAAAAGC AATACAGCTT GAAAGATTTC 
CTTTTTGAAT TGTCTTGCTG CCCACATGAA AGAAATGTTG AAATCTACCA GTATATACTG 
AGAAACCCAG AGAAAGTGCT GCTTATCTTT GATGGTGTTG ATGAGTTTCA AGACTCAGAG 
GGTCTGATGC AGGCTTCTTA CACCGCATCA CCTACAAAGA CACATAAAAT AAAGGAATTA 
TTTACTGGCC TTTTCCAAAA GAAGCTGCTA CGGGGGTGCA CCATGCTAAT AACTGCAAGA 
CCAAAAGATA AATTTAATCA GTACTTAACT AAGGTAGACC AGATTGTGGA AATGTCTGGA 
TTTTCTCAGC AGCAGGTGGA ATGGTATGTC AATGAATATT TTAAGGGGTT TCAAGATTTT 
ACTGACGTGC TAAAATGGAT GAGAGAATAT GAATACTTAT TCAGATATTG TTATATCCCC 
TTTATGTGTA GGATAATGTG TCTGTTTGCT GAAACTAATT TTAGGGATGA GAGAGTGGAA 
TCTCATTTTT CTTTAGCAGA ATTTATGTTG ACTGTGCTTC AGAGAAACGA AGAGTTTACA 
GGCCAAGGTT CCTCAGGTTT GTCATCTGAT GAGCATTCCA ATATGAAAGG TGATCAAGCT 
GTACAATGTG CCTCAACCCC TCAGCCTTCT TTAATGGAAA ATGAGATGGA TTTATCTAAT 
GGAGTTCATA CTAAACACAA ACTTCCAGTA AAAAAACACC ATATAAACAA CGGGAATTCA 
CTGGTTCAGC ATTTTCGAAC AGCTCAAAGT CTTATGGAAC ATATAACAGA CAAGCATTTT 
GTCAGATATG CTTCACTGGA GCTCAAAAAG AAACGAAATC ATGATAATTG CACTGATATG 
GTACGGAGGT GCTTGGTTGG ACTTTTATTT CGTAAAGATT TAAATACTGA TCTGCTGAAA 
GTCATTCTCA AAAAACAAAA GAAAATGACT GATTACTTCA AGGCAATACA GCCTGAAACA 
CTTTGTCCAC AAAGACTACT TGAACTTTGT CACTGTGTCT GTGAGACAAA TGACATTAGT 
CTAAGTCAAC ACACGGCTCA AAAACTTAGT AATGAACTTT CATTTGTGGG TACCAGACTT 
ACGCCCCCAG ATGTTTATGT GTTAAAATGC ATTTTGAAGC ATTCACACAC AAAAATATCC 
TTGGACCTAA GGAGAACAGG AATTGATCAA CAAGGATTAA AGGAATTGGT AAAACTAAAA 
ACCATCAAGT CATTCAGGGC ATCTCTCAGT GACACGGTCA GACTCTGGAA GACCTTACAA 
ACTGAGAACA ACCTCAGACT GTTAAAGCTG TGTGTTATGA AATTCAGAAT CGATCCATTC 
ACAGCAGAAA GCATAAAAGA TATAACTGAT CTTACAGCCA TTGTCAGTAT TCAGAATGAA 
ATTTGCAGTT GCCTTCAAGA ATTAACAGAT GGGATCTCTG AAATTCCAGC TATCAAAACA 
CTTAAAAAGA TCATATTTAG ACTTGGAAAG AGTCATGGAC AAAATGGGTT CATGAGGCTT 
GTTGATATCC TTCCACAGCT TCCATCTCTC CAACATTTAG ATTTACATTC TCTTATTGAG 
AATCACATCG GTGATAAATC TGTTGCAAAG CTGGCCGAGA AATTCCCAGA AATGCTGTCT 
CTTGAGACAC TGGACTTATC ACAAAACACC ATAACCGATG TCGGTGCAGC AAAGCTGGCC 
ATGGCTCTTC CTTCCTTGGG CTCGCTACAT ACACTTAGTT TATTCAACAA CTTTATCCAG 
GATGATGGAG CCAAGAAATT TGCTGAGATA CTTCCTGAAA TGTCTTCCCT GGAAAAGCTA 
CATCTAGCAT ACAACAAGAT CAGTGATGTT GGTGCCAGGA ATCTCACAGA GAGTCTTAGG 
AAATGTCCCA AAATGAAGAG TTTGATGATA TACAGTTCCG CAATTCCTCA TAATATTTTG 
CATCATCTCC AACAGCAGGA CCCAAGGATC AGTTGCCAGT CGTTTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952759.2
CDS	45..3815
Translation	MNYFKEILPFTTQIVSNSSSNDLRKLLSQLVETKIISMEYYVSLLSENDTDDLARKIALIAVEKPEIRHKFPSLFCGPEREDYPLEAPAVSMDSRQENLQLPRKTVSNSFSNTITQFPNNSLETQTTPLEYFLNSLSENNMDNLTLDMSLKFAERNEILHTCLPLMDELEEEYIFTDATSFRENSMMDLSTIDSSVLNWSLESESELDLETLSTLDLSDIEKCFLDDPLDFELEDDNCSNCIDNDEEYDKIARICNYICGEDHQMESLLDGESGNPAEAPNNETLSKKLKRKRKIGPVIPKRKRINREKPTAPQCMEATEAQDTPDILPTSKSLSAPVHISSLPVIQVFGKLPPEFTPQHIISLPPGANIIFYTAPSSVSPPFQAQPAPPVSTVTISEIPTVPMSIPCSPDKNCSFSPFTPAAADLPNSPEATAMSQYPLKENETVTPQSLAPMENSFAEKEKNKYVDLFKAALTEKYKNLWAFERDVLYTDIALVKSRIDPKPGKVTCKTAEKELVIYDLDEREKEAVDHFSLFADKERKEMETNIIALLGQSGMGKTVLAKKICQDWSNGAYSQFSLVIFFEWSMLASCKKQYSLKDFLFELSCCPHERNVEIYQYILRNPEKVLLIFDGVDEFQDSEGLMQASYTASPTKTHKIKELFTGLFQKKLLRGCTMLITARPKDKFNQYLTKVDQIVEMSGFSQQQVEWYVNEYFKGFQDFTDVLKWMREYEYLFRYCYIPFMCRIMCLFAETNFRDERVESHFSLAEFMLTVLQRNEEFTGQGSSGLSSDEHSNMKGDQAVQCASTPQPSLMENEMDLSNGVHTKHKLPVKKHHINNGNSLVQHFRTAQSLMEHITDKHFVRYASLELKKKRNHDNCTDMVRRCLVGLLFRKDLNTDLLKVILKKQKKMTDYFKAIQPETLCPQRLLELCHCVCETNDISLSQHTAQKLSNELSFVGTRLTPPDVYVLKCILKHSHTKISLDLRRTGIDQQGLKELVKLKTIKSFRASLSDTVRLWKTLQTENNLRLLKLCVMKFRIDPFTAESIKDITDLTAIVSIQNEICSCLQELTDGISEIPAIKTLKKIIFRLGKSHGQNGFMRLVDILPQLPSLQHLDLHSLIENHIGDKSVAKLAEKFPEMLSLETLDLSQNTITDVGAAKLAMALPSLGSLHTLSLFNNFIQDDGAKKFAEILPEMSSLEKLHLAYNKISDVGARNLTESLRKCPKMKSLMIYSSAIPHNILHHLQQQDPRISCQSFG

Target ORF information:

RefSeq Version	XM_018097270.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis class II, major histocompatibility complex, transactivator (ciita), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097270.2

ORF Insert Sequence:

ATGAATTACT TTAAGGAGAT CCTGCCTTTT ACGACACAAA TTGTCTCCAA CTCCTCCTCT 
AACGACCTAA GAAAACTTCT TAGTCAGCTT GTTGAAACCA AGATTATATC CATGGAATAT 
TATGTTAGTT TGCTAAGTGA AAATGACACA GATGATCTTG CACGGAAAAT TGCTTTGATT 
GCTGTAGAAA AACCTGAAAT TCGCCATAAA TTCCCATCAC TTTTCTGTGG ACCTGAGAGG 
GAAGACTATC CACTAGAGGC ACCTGCAGTT AGTATGGATT CAAGGCAGGA GAACCTACAG 
TTACCCAGAA AGACTGTCTC TAACTCATTC TCTAATACCA TAACTCAATT TCCCAATAAT 
TCTCTGGAAA CACAGACCAC GCCACTGGAA TATTTTCTAA ATTCACTCAG TGAGAACAAT 
ATGGATAATC TTACATTGGA TATGTCCTTA AAATTTGCAG AAAGAAATGA AATACTTCAT 
ACATGTTTAC CACTTATGGA TGAACTAGAA GAAGAATATA TTTTCACTGA TGCCACTTCA 
TTTAGGGAAA ATTCAATGAT GGATCTGAGC ACTATAGATA GTAGTGTGCT GAATTGGAGT 
TTGGAATCGG AGTCAGAATT GGACTTGGAG ACACTGTCCA CTTTGGATTT ATCAGATATT 
GAAAAGTGTT TTTTAGATGA CCCTTTGGAT TTTGAACTTG AAGATGATAA CTGCAGTAAT 
TGTATTGATA ATGATGAAGA ATATGACAAG ATTGCCAGAA TATGCAATTA TATTTGTGGT 
GAAGATCATC AGATGGAAAG TTTATTGGAT GGAGAATCAG GGAATCCTGC AGAAGCCCCA 
AACAACGAAA CATTATCAAA GAAATTAAAA AGAAAAAGAA AAATTGGGCC AGTGATACCA 
AAGCGAAAGA GGATAAACCG AGAAAAACCG ACAGCCCCTC AGTGTATGGA GGCAACAGAA 
GCTCAGGATA CCCCAGACAT TCTCCCAACA TCTAAAAGTC TCAGTGCACC CGTGCACATA 
TCTTCACTAC CTGTAATCCA GGTCTTTGGT AAATTGCCAC CAGAATTCAC GCCCCAGCAT 
ATAATCAGTT TGCCTCCTGG AGCAAATATT ATTTTCTACA CAGCACCTTC TTCAGTGTCC 
CCGCCATTCC AAGCACAACC TGCGCCTCCA GTTTCCACAG TTACCATTTC AGAAATTCCA 
ACAGTTCCTA TGAGCATCCC ATGTTCCCCC GACAAAAACT GCTCCTTTAG TCCATTCACT 
CCTGCAGCAG CTGATTTACC AAATTCCCCA GAAGCAACTG CCATGAGTCA GTACCCTCTG 
AAAGAAAATG AAACTGTTAC CCCACAGTCA CTAGCACCTA TGGAGAATTC ATTTGCAGAG 
AAAGAAAAGA ATAAATATGT GGACTTGTTT AAAGCTGCAT TGACAGAGAA GTACAAAAAC 
TTATGGGCCT TTGAAAGAGA TGTATTGTAC ACTGACATTG CACTTGTGAA AAGCAGGATT 
GACCCCAAAC CTGGGAAAGT TACATGTAAA ACAGCAGAGA AGGAACTTGT CATTTATGAT 
TTAGATGAAC GGGAAAAAGA AGCAGTGGAT CATTTCAGTC TTTTTGCAGA TAAAGAAAGG 
AAAGAGATGG AGACAAATAT AATTGCACTA CTGGGACAAT CAGGAATGGG GAAAACTGTT 
CTTGCTAAAA AAATATGCCA AGACTGGTCC AATGGCGCTT ATTCACAGTT CAGTCTAGTG 
ATTTTTTTTG AGTGGAGCAT GCTTGCTTCT TGTAAAAAGC AATACAGCTT GAAAGATTTC 
CTTTTTGAAT TGTCTTGCTG CCCACATGAA AGAAATGTTG AAATCTACCA GTATATACTG 
AGAAACCCAG AGAAAGTGCT GCTTATCTTT GATGGTGTTG ATGAGTTTCA AGACTCAGAG 
GGTCTGATGC AGGCTTCTTA CACCGCATCA CCTACAAAGA CACATAAAAT AAAGGAATTA 
TTTACTGGCC TTTTCCAAAA GAAGCTGCTA CGGGGGTGCA CCATGCTAAT AACTGCAAGA 
CCAAAAGATA AATTTAATCA GTACTTAACT AAGGTAGACC AGATTGTGGA AATGTCTGGA 
TTTTCTCAGC AGCAGGTGGA ATGGTATGTC AATGAATATT TTAAGGGGTT TCAAGATTTT 
ACTGACGTGC TAAAATGGAT GAGAGAATAT GAATACTTAT TCAGATATTG TTATATCCCC 
TTTATGTGTA GGATAATGTG TCTGTTTGCT GAAACTAATT TTAGGGATGA GAGAGTGGAA 
TCTCATTTTT CTTTAGCAGA ATTTATGTTG ACTGTGCTTC AGAGAAACGA AGAGTTTACA 
GGCCAAGGTT CCTCAGGTTT GTCATCTGAT GAGCATTCCA ATATGAAAGG TGATCAAGCT 
GTACAATGTG CCTCAACCCC TCAGCCTTCT TTAATGGAAA ATGAGATGGA TTTATCTAAT 
GGAGTTCATA CTAAACACAA ACTTCCAGTA AAAAAACACC ATATAAACAA CGGGAATTCA 
CTGGTTCAGC ATTTTCGAAC AGCTCAAAGT CTTATGGAAC ATATAACAGA CAAGCATTTT 
GTCAGATATG CTTCACTGGA GCTCAAAAAG AAACGAAATC ATGATAATTG CACTGATATG 
GTACGGAGGT GCTTGGTTGG ACTTTTATTT CGTAAAGATT TAAATACTGA TCTGCTGAAA 
GTCATTCTCA AAAAACAAAA GAAAATGACT GATTACTTCA AGGCAATACA GCCTGAAACA 
CTTTGTCCAC AAAGACTACT TGAACTTTGT CACTGTGTCT GTGAGACAAA TGACATTAGT 
CTAAGTCAAC ACACGGCTCA AAAACTTAGT AATGAACTTT CATTTGTGGG TACCAGACTT 
ACGCCCCCAG ATGTTTATGT GTTAAAATGC ATTTTGAAGC ATTCACACAC AAAAATATCC 
TTGGACCTAA GGAGAACAGG AATTGATCAA CAAGGATTAA AGGAATTGGT AAAACTAAAA 
ACCATCAAGT CATTCAGGGC ATCTCTCAGT GACACGGTCA GACTCTGGAA GACCTTACAA 
ACTGAGAACA ACCTCAGACT GTTAAAGCTG TGTGTTATGA AATTCAGAAT CGATCCATTC 
ACAGCAGAAA GCATAAAAGA TATAACTGAT CTTACAGCCA TTGTCAGTAT TCAGAATGAA 
ATTTGCAGTT GCCTTCAAGA ATTAACAGAT GGGATCTCTG AAATTCCAGC TATCAAAACA 
CTTAAAAAGA TCATATTTAG ACTTGGAAAG AGTCATGGAC AAAATGGGTT CATGAGGCTT 
GTTGATATCC TTCCACAGCT TCCATCTCTC CAACATTTAG ATTTACATTC TCTTATTGAG 
AATCACATCG GTGATAAATC TGTTGCAAAG CTGGCCGAGA AATTCCCAGA AATGCTGTCT 
CTTGAGACAC TGGACTTATC ACAAAACACC ATAACCGATG TCGGTGCAGC AAAGCTGGCC 
ATGGCTCTTC CTTCCTTGGG CTCGCTACAT ACACTTAGTT TATTCAACAA CTTTATCCAG 
GATGATGGAG CCAAGAAATT TGCTGAGATA CTTCCTGAAA TGTCTTCCCT GGAAAAGCTA 
CATCTAGCAT ACAACAAGAT CAGTGATGTT GGTGCCAGGA ATCTCACAGA GAGTCTTAGG 
AAATGTCCCA AAATGAAGAG TTTGATGATA TACAGTTCCG CAATTCCTCA TAATATTTTG 
CATCATCTCC AACAGCAGGA CCCAAGGATC AGTTGCCAGT CGTTTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ciita cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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