ORF » Species Summary » Xenopus tropicalis » gbf1 cDNA ORF clone

gbf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following gbf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gbf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa12833 XM_018095871.1
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa12834 XM_018095872.1
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa12835 XM_018095873.1
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa33097 XM_012966854.2
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa33097 XM_012966852.2
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa33097 XM_012966853.2
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa33097 XM_012966851.2
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62534 XM_031906081.1
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62534 XM_031906082.1
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62534 XM_031906080.1
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62535 XM_031906083.1
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62536 XM_031906084.1
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62537 XM_031906085.1
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62538 XM_012966851.3
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62538 XM_012966852.3
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62539 XM_031906086.1
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa62540 XM_018095873.2
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa62541 XM_018095871.2
Latest version!		 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    gbf1 ( XM_018095871.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951360.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OXa12833
    Clone ID Related Accession (Same CDS sequence) XM_018095871.1
    Accession Version XM_018095871.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA 
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG 
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC 
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA 
    TTACAGGTTA GTCATGATCT TTTGGACCTA ATGCACACAC TTCACACAAG AGCTGCCACC 
    ATATACAGTT CTTGGGCAGA GGAACAGCGC AACCTAGAGA CAGCTGCTGA GAAAATTACA 
    GCCGATTCTA AGACACTGTG GTCAAACTGT TGGTGTCCTT TACTGCAAGG TATTGCTTGG 
    CTCTGCTGTG ATGCTCGGCG CCAGGTCAGA ATGCAGGCTC TCACCTACCT GCAAAGGGCA 
    CTTTTAGTCC ATGACTTACA GGCTCTTGAT GCACTTGAAT GGGAATCATG CTTTAATAAG 
    GTGCTATTTC CATTGCTGAC CAAACTATTG GAAAATATTA GTCCAGCTGA TGTTGGCGGG 
    ATGGAGGAGA CCAGAATGAG AGGCTCCACT CTGCTCTCAA AGGTATTTCT ACAGCATCTG 
    TCTCCTCTTC TTTCTCTGTC GACATTTGCT GCCTTGTGGC TTACTATTCT TGATTTCATG 
    GACAAGTACA TGCATGCTGG CTCCAGTGAC TTACTGCCTG AGGCAATTCC AGAGTCACTG 
    AAAAATATGC TTCTCGTAAT GGACACTGCC GGAATATTCC ACAGTGCAGA CTCGCGGACG 
    GGCTACTCTG ATTTATGGGA GATCACGTGG GAAAGGATAG ATTGCTTTTT ACCTCGTCTA 
    AGGGAAGAAC TGTTTAAACA AACTGTGATA CAAGATCCAC CCAATATAGT TACAACAGAA 
    ACTCCTGTTG AGCCAAGACC TCCCCCTCAA TCAACTCAGT CAGAGAGACC AGCGGAGCAG 
    GGACCTTGTG ACTCAGAGAC TCAAAGTTTA GCAATCTCAT CTGCTCCAGT AGTTGGATCA 
    AGTTCTTCCT GCACCCCAGT TAGAGTCAGC CCTGTAACAG ATATACCTCC ACCTATAACT 
    CAGTCTCCAG TCATCCTGCA TCCTCTGACC TCCCCGCTGC AGGTGGGAGT GCCACCCATG 
    TCGCTGCCAA TCATCCTCAA CCCTGCATTA ATTGAAGCTA CCTCTCCAGT TCCGCTCTTG 
    TCCACTCCCC GACCTGCTGA TGTATCATCT GTCTCAGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951360.1
    CDS163..5730
    Translation

    Target ORF information:

    RefSeq Version XM_018095871.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_018095871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA 
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG 
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC 
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA 
    TTACAGGTTA GTCATGATCT TTTGGACCTA ATGCACACAC TTCACACAAG AGCTGCCACC 
    ATATACAGTT CTTGGGCAGA GGAACAGCGC AACCTAGAGA CAGCTGCTGA GAAAATTACA 
    GCCGATTCTA AGACACTGTG GTCAAACTGT TGGTGTCCTT TACTGCAAGG TATTGCTTGG 
    CTCTGCTGTG ATGCTCGGCG CCAGGTCAGA ATGCAGGCTC TCACCTACCT GCAAAGGGCA 
    CTTTTAGTCC ATGACTTACA GGCTCTTGAT GCACTTGAAT GGGAATCATG CTTTAATAAG 
    GTGCTATTTC CATTGCTGAC CAAACTATTG GAAAATATTA GTCCAGCTGA TGTTGGCGGG 
    ATGGAGGAGA CCAGAATGAG AGGCTCCACT CTGCTCTCAA AGGTATTTCT ACAGCATCTG 
    TCTCCTCTTC TTTCTCTGTC GACATTTGCT GCCTTGTGGC TTACTATTCT TGATTTCATG 
    GACAAGTACA TGCATGCTGG CTCCAGTGAC TTACTGCCTG AGGCAATTCC AGAGTCACTG 
    AAAAATATGC TTCTCGTAAT GGACACTGCC GGAATATTCC ACAGTGCAGA CTCGCGGACG 
    GGCTACTCTG ATTTATGGGA GATCACGTGG GAAAGGATAG ATTGCTTTTT ACCTCGTCTA 
    AGGGAAGAAC TGTTTAAACA AACTGTGATA CAAGATCCAC CCAATATAGT TACAACAGAA 
    ACTCCTGTTG AGCCAAGACC TCCCCCTCAA TCAACTCAGT CAGAGAGACC AGCGGAGCAG 
    GGACCTTGTG ACTCAGAGAC TCAAAGTTTA GCAATCTCAT CTGCTCCAGT AGTTGGATCA 
    AGTTCTTCCT GCACCCCAGT TAGAGTCAGC CCTGTAACAG ATATACCTCC ACCTATAACT 
    CAGTCTCCAG TCATCCTGCA TCCTCTGACC TCCCCGCTGC AGGTGGGAGT GCCACCCATG 
    TCGCTGCCAA TCATCCTCAA CCCTGCATTA ATTGAAGCTA CCTCTCCAGT TCCGCTCTTG 
    TCCACTCCCC GACCTGCTGA TGTATCATCT GTCTCAGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_018095872.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951361.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OXa12834
    Clone ID Related Accession (Same CDS sequence) XM_018095872.1
    Accession Version XM_018095872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGCTTTTGGA CCTAATGCAC ACACTTCACA CAAGAGCTGC CACCATATAC 
    AGTTCTTGGG CAGAGGAACA GCGCAACCTA GAGACAGCTG CTGAGAAAAT TACAGCCGAT 
    TCTAAGACAC TGTGGTCAAA CTGTTGGTGT CCTTTACTGC AAGGTATTGC TTGGCTCTGC 
    TGTGATGCTC GGCGCCAGGT CAGAATGCAG GCTCTCACCT ACCTGCAAAG GGCACTTTTA 
    GTCCATGACT TACAGGCTCT TGATGCACTT GAATGGGAAT CATGCTTTAA TAAGGTGCTA 
    TTTCCATTGC TGACCAAACT ATTGGAAAAT ATTAGTCCAG CTGATGTTGG CGGGATGGAG 
    GAGACCAGAA TGAGAGGCTC CACTCTGCTC TCAAAGGTAT TTCTACAGCA TCTGTCTCCT 
    CTTCTTTCTC TGTCGACATT TGCTGCCTTG TGGCTTACTA TTCTTGATTT CATGGACAAG 
    TACATGCATG CTGGCTCCAG TGACTTACTG CCTGAGGCAA TTCCAGAGTC ACTGAAAAAT 
    ATGCTTCTCG TAATGGACAC TGCCGGAATA TTCCACAGTG CAGACTCGCG GACGGGCTAC 
    TCTGATTTAT GGGAGATCAC GTGGGAAAGG ATAGATTGCT TTTTACCTCG TCTAAGGGAA 
    GAACTGTTTA AACAAACTGT GATACAAGAT CCACCCAATA TAGTTACAAC AGAAACTCCT 
    GTTGAGCCAA GACCTCCCCC TCAATCAACT CAGTCAGAGA GACCAGCGGA GCAGGGACCT 
    TGTGACTCAG AGACTCAAAG TTTAGCAATC TCATCTGCTC CAGTAGTTGG ATCAAGTTCT 
    TCCTGCACCC CAGTTAGAGT CAGCCCTGTA ACAGATATAC CTCCACCTAT AACTCAGTCT 
    CCAGTCATCC TGCATCCTCT GACCTCCCCG CTGCAGGTGG GAGTGCCACC CATGTCGCTG 
    CCAATCATCC TCAACCCTGC ATTAATTGAA GCTACCTCTC CAGTTCCGCT CTTGTCCACT 
    CCCCGACCTG CTGATGTATC ATCTGTCTCA GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951361.1
    CDS162..5723
    Translation

    Target ORF information:

    RefSeq Version XM_018095872.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_018095872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGCTTTTGGA CCTAATGCAC ACACTTCACA CAAGAGCTGC CACCATATAC 
    AGTTCTTGGG CAGAGGAACA GCGCAACCTA GAGACAGCTG CTGAGAAAAT TACAGCCGAT 
    TCTAAGACAC TGTGGTCAAA CTGTTGGTGT CCTTTACTGC AAGGTATTGC TTGGCTCTGC 
    TGTGATGCTC GGCGCCAGGT CAGAATGCAG GCTCTCACCT ACCTGCAAAG GGCACTTTTA 
    GTCCATGACT TACAGGCTCT TGATGCACTT GAATGGGAAT CATGCTTTAA TAAGGTGCTA 
    TTTCCATTGC TGACCAAACT ATTGGAAAAT ATTAGTCCAG CTGATGTTGG CGGGATGGAG 
    GAGACCAGAA TGAGAGGCTC CACTCTGCTC TCAAAGGTAT TTCTACAGCA TCTGTCTCCT 
    CTTCTTTCTC TGTCGACATT TGCTGCCTTG TGGCTTACTA TTCTTGATTT CATGGACAAG 
    TACATGCATG CTGGCTCCAG TGACTTACTG CCTGAGGCAA TTCCAGAGTC ACTGAAAAAT 
    ATGCTTCTCG TAATGGACAC TGCCGGAATA TTCCACAGTG CAGACTCGCG GACGGGCTAC 
    TCTGATTTAT GGGAGATCAC GTGGGAAAGG ATAGATTGCT TTTTACCTCG TCTAAGGGAA 
    GAACTGTTTA AACAAACTGT GATACAAGAT CCACCCAATA TAGTTACAAC AGAAACTCCT 
    GTTGAGCCAA GACCTCCCCC TCAATCAACT CAGTCAGAGA GACCAGCGGA GCAGGGACCT 
    TGTGACTCAG AGACTCAAAG TTTAGCAATC TCATCTGCTC CAGTAGTTGG ATCAAGTTCT 
    TCCTGCACCC CAGTTAGAGT CAGCCCTGTA ACAGATATAC CTCCACCTAT AACTCAGTCT 
    CCAGTCATCC TGCATCCTCT GACCTCCCCG CTGCAGGTGG GAGTGCCACC CATGTCGCTG 
    CCAATCATCC TCAACCCTGC ATTAATTGAA GCTACCTCTC CAGTTCCGCT CTTGTCCACT 
    CCCCGACCTG CTGATGTATC ATCTGTCTCA GAGGTCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_018095873.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951362.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OXa12835
    Clone ID Related Accession (Same CDS sequence) XM_018095873.1
    Accession Version XM_018095873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA 
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG 
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC 
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA 
    TTACAGCTTT TGGACCTAAT GCACACACTT CACACAAGAG CTGCCACCAT ATACAGTTCT 
    TGGGCAGAGG AACAGCGCAA CCTAGAGACA GCTGCTGAGA AAATTACAGC CGATTCTAAG 
    ACACTGTGGT CAAACTGTTG GTGTCCTTTA CTGCAAGGTA TTGCTTGGCT CTGCTGTGAT 
    GCTCGGCGCC AGGTCAGAAT GCAGGCTCTC ACCTACCTGC AAAGGGCACT TTTAGTCCAT 
    GACTTACAGG CTCTTGATGC ACTTGAATGG GAATCATGCT TTAATAAGGT GCTATTTCCA 
    TTGCTGACCA AACTATTGGA AAATATTAGT CCAGCTGATG TTGGCGGGAT GGAGGAGACC 
    AGAATGAGAG GCTCCACTCT GCTCTCAAAG GTATTTCTAC AGCATCTGTC TCCTCTTCTT 
    TCTCTGTCGA CATTTGCTGC CTTGTGGCTT ACTATTCTTG ATTTCATGGA CAAGTACATG 
    CATGCTGGCT CCAGTGACTT ACTGCCTGAG GCAATTCCAG AGTCACTGAA AAATATGCTT 
    CTCGTAATGG ACACTGCCGG AATATTCCAC AGTGCAGACT CGCGGACGGG CTACTCTGAT 
    TTATGGGAGA TCACGTGGGA AAGGATAGAT TGCTTTTTAC CTCGTCTAAG GGAAGAACTG 
    TTTAAACAAA CTGTGATACA AGATCCACCC AATATAGTTA CAACAGAAAC TCCTGTTGAG 
    CCAAGACCTC CCCCTCAATC AACTCAGTCA GAGAGACCAG CGGAGCAGGG ACCTTGTGAC 
    TCAGAGACTC AAAGTTTAGC AATCTCATCT GCTCCAGTAG TTGGATCAAG TTCTTCCTGC 
    ACCCCAGTTA GAGTCAGCCC TGTAACAGAT ATACCTCCAC CTATAACTCA GTCTCCAGTC 
    ATCCTGCATC CTCTGACCTC CCCGCTGCAG GTGGGAGTGC CACCCATGTC GCTGCCAATC 
    ATCCTCAACC CTGCATTAAT TGAAGCTACC TCTCCAGTTC CGCTCTTGTC CACTCCCCGA 
    CCTGCTGATG TATCATCTGT CTCAGAGGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951362.1
    CDS162..5717
    Translation

    Target ORF information:

    RefSeq Version XM_018095873.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_018095873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA 
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG 
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC 
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA 
    TTACAGCTTT TGGACCTAAT GCACACACTT CACACAAGAG CTGCCACCAT ATACAGTTCT 
    TGGGCAGAGG AACAGCGCAA CCTAGAGACA GCTGCTGAGA AAATTACAGC CGATTCTAAG 
    ACACTGTGGT CAAACTGTTG GTGTCCTTTA CTGCAAGGTA TTGCTTGGCT CTGCTGTGAT 
    GCTCGGCGCC AGGTCAGAAT GCAGGCTCTC ACCTACCTGC AAAGGGCACT TTTAGTCCAT 
    GACTTACAGG CTCTTGATGC ACTTGAATGG GAATCATGCT TTAATAAGGT GCTATTTCCA 
    TTGCTGACCA AACTATTGGA AAATATTAGT CCAGCTGATG TTGGCGGGAT GGAGGAGACC 
    AGAATGAGAG GCTCCACTCT GCTCTCAAAG GTATTTCTAC AGCATCTGTC TCCTCTTCTT 
    TCTCTGTCGA CATTTGCTGC CTTGTGGCTT ACTATTCTTG ATTTCATGGA CAAGTACATG 
    CATGCTGGCT CCAGTGACTT ACTGCCTGAG GCAATTCCAG AGTCACTGAA AAATATGCTT 
    CTCGTAATGG ACACTGCCGG AATATTCCAC AGTGCAGACT CGCGGACGGG CTACTCTGAT 
    TTATGGGAGA TCACGTGGGA AAGGATAGAT TGCTTTTTAC CTCGTCTAAG GGAAGAACTG 
    TTTAAACAAA CTGTGATACA AGATCCACCC AATATAGTTA CAACAGAAAC TCCTGTTGAG 
    CCAAGACCTC CCCCTCAATC AACTCAGTCA GAGAGACCAG CGGAGCAGGG ACCTTGTGAC 
    TCAGAGACTC AAAGTTTAGC AATCTCATCT GCTCCAGTAG TTGGATCAAG TTCTTCCTGC 
    ACCCCAGTTA GAGTCAGCCC TGTAACAGAT ATACCTCCAC CTATAACTCA GTCTCCAGTC 
    ATCCTGCATC CTCTGACCTC CCCGCTGCAG GTGGGAGTGC CACCCATGTC GCTGCCAATC 
    ATCCTCAACC CTGCATTAAT TGAAGCTACC TCTCCAGTTC CGCTCTTGTC CACTCCCCGA 
    CCTGCTGATG TATCATCTGT CTCAGAGGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_012966852.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822306.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa33097
    Clone ID Related Accession (Same CDS sequence) XM_012966852.2 , XM_012966854.2 , XM_012966853.2 , XM_012966851.2
    Accession Version XM_012966852.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822306.1
    CDS163..5736
    Translation

    Target ORF information:

    RefSeq Version XM_012966852.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012966852.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_012966854.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822308.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OXa33097
    Clone ID Related Accession (Same CDS sequence) XM_012966852.2 , XM_012966854.2 , XM_012966853.2 , XM_012966851.2
    Accession Version XM_012966854.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822308.1
    CDS35..5608
    Translation

    Target ORF information:

    RefSeq Version XM_012966854.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012966854.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_012966853.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822307.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OXa33097
    Clone ID Related Accession (Same CDS sequence) XM_012966852.2 , XM_012966854.2 , XM_012966853.2 , XM_012966851.2
    Accession Version XM_012966853.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822307.1
    CDS84..5657
    Translation

    Target ORF information:

    RefSeq Version XM_012966853.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012966853.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_012966851.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822305.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OXa33097
    Clone ID Related Accession (Same CDS sequence) XM_012966852.2 , XM_012966854.2 , XM_012966853.2 , XM_012966851.2
    Accession Version XM_012966851.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1473177600000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966851.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822305.1
    CDS337..5910
    Translation

    Target ORF information:

    RefSeq Version XM_012966851.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012966851.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCTAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATTGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_031906080.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761940.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa62534
    Clone ID Related Accession (Same CDS sequence) XM_031906080.1 , XM_031906081.1 , XM_031906082.1
    Accession Version XM_031906080.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG 
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA 
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT 
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT 
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG 
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA 
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT 
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC 
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG 
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA 
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT 
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT 
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC 
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA 
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT 
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA 
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA 
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC 
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC 
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC 
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT 
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761940.1
    CDS100..5772
    Translation

    Target ORF information:

    RefSeq Version XM_031906080.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031906080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG 
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA 
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT 
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT 
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG 
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA 
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT 
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC 
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG 
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA 
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT 
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT 
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC 
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA 
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT 
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA 
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA 
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC 
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC 
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC 
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT 
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_031906081.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761941.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OXa62534
    Clone ID Related Accession (Same CDS sequence) XM_031906080.1 , XM_031906081.1 , XM_031906082.1
    Accession Version XM_031906081.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG 
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA 
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT 
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT 
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG 
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA 
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT 
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC 
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG 
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA 
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT 
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT 
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC 
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA 
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT 
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA 
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA 
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC 
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC 
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC 
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT 
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761941.1
    CDS263..5935
    Translation

    Target ORF information:

    RefSeq Version XM_031906081.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031906081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG 
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA 
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT 
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT 
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG 
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA 
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT 
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC 
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG 
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA 
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT 
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT 
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC 
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA 
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT 
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA 
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA 
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC 
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC 
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC 
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT 
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_031906082.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761942.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OXa62534
    Clone ID Related Accession (Same CDS sequence) XM_031906080.1 , XM_031906081.1 , XM_031906082.1
    Accession Version XM_031906082.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5673bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG 
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA 
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT 
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT 
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG 
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA 
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT 
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC 
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG 
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA 
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT 
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT 
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC 
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA 
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT 
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA 
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA 
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC 
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC 
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC 
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT 
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761942.1
    CDS86..5758
    Translation

    Target ORF information:

    RefSeq Version XM_031906082.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031906082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG 
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA 
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT 
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GGTTAGTCAT 
    GATCTTTTGG ACCTAATGCA CACACTTCAC ACAAGAGCTG CCACCATATA CAGTTCTTGG 
    GCAGAGGAAC AGCGCAACCT AGAGACAGCT GCTGAGAAAA TTACAGCCGA TTCTAAGACA 
    CTGTGGTCAA ACTGTTGGTG TCCTTTACTG CAAGGTATTG CTTGGCTCTG CTGTGATGCT 
    CGGCGCCAGG TCAGAATGCA GGCTCTCACC TACCTGCAAA GGGCACTTTT AGTCCATGAC 
    TTACAGGCTC TTGATGCACT TGAATGGGAA TCATGCTTTA ATAAGGTGCT ATTTCCATTG 
    CTGACCAAAC TATTGGAAAA TATTAGTCCA GCTGATGTTG GCGGGATGGA GGAGACCAGA 
    ATGAGAGGCT CCACTCTGCT CTCAAAGGTA TTTCTACAGC ATCTGTCTCC TCTTCTTTCT 
    CTGTCGACAT TTGCTGCCTT GTGGCTTACT ATTCTTGATT TCATGGACAA GTACATGCAT 
    GCTGGCTCCA GTGACTTACT GCCTGAGGCA ATTCCAGAGT CACTGAAAAA TATGCTTCTC 
    GTAATGGACA CTGCCGGAAT ATTCCACAGT GCAGACTCGC GGACGGGCTA CTCTGATTTA 
    TGGGAGATCA CGTGGGAAAG GATAGATTGC TTTTTACCTC GTCTAAGGGA AGAACTGTTT 
    AAACAAACTG TGATACAAGA TCCACCCAAT ATAGTTACAA CAGAAACTCC TGTTGAGCCA 
    AGACCTCCCC CTCAATCAAC TCAGTCAGAG AGACCAGCGG AGCAGGGACC TTGTGACTCA 
    GAGACTCAAA GTTTAGCAAT CTCATCTGCT CCAGTAGTTG GATCAAGTTC TTCCTGCACC 
    CCAGTTAGAG TCAGCCCTGT AACAGATATA CCTCCACCTA TAACTCAGTC TCCAGTCATC 
    CTGCATCCTC TGACCTCCCC GCTGCAGGTG GGAGTGCCAC CCATGTCGCT GCCAATCATC 
    CTCAACCCTG CATTAATTGA AGCTACCTCT CCAGTTCCGC TCTTGTCCAC TCCCCGACCT 
    GCTGATGTAT CATCTGTCTC AGAGGTCAAC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_031906083.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761943.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OXa62535
    Clone ID Related Accession (Same CDS sequence) XM_031906083.1
    Accession Version XM_031906083.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGGTACAAAT CTCAGGAGAA AAGGGTAAAG 
    AACCACAAAT ATGACAGTAA ATCCAACCGC TTCAAAAAGA AAACCAAGGA AAAAGAAAAT 
    TCTGTCCGCC GTACACGGGC TTCAAATCAG CGACAATACC GATCTCACAG TGATGATGAG 
    GAAGATGAAA GTGTACCAGC CAGCTATCAC ACTGTGTCAT TACAGGTTAG TCATGATCTT 
    TTGGACCTAA TGCACACACT TCACACAAGA GCTGCCACCA TATACAGTTC TTGGGCAGAG 
    GAACAGCGCA ACCTAGAGAC AGCTGCTGAG AAAATTACAG CCGATTCTAA GACACTGTGG 
    TCAAACTGTT GGTGTCCTTT ACTGCAAGGT ATTGCTTGGC TCTGCTGTGA TGCTCGGCGC 
    CAGGTCAGAA TGCAGGCTCT CACCTACCTG CAAAGGGCAC TTTTAGTCCA TGACTTACAG 
    GCTCTTGATG CACTTGAATG GGAATCATGC TTTAATAAGG TGCTATTTCC ATTGCTGACC 
    AAACTATTGG AAAATATTAG TCCAGCTGAT GTTGGCGGGA TGGAGGAGAC CAGAATGAGA 
    GGCTCCACTC TGCTCTCAAA GGTATTTCTA CAGCATCTGT CTCCTCTTCT TTCTCTGTCG 
    ACATTTGCTG CCTTGTGGCT TACTATTCTT GATTTCATGG ACAAGTACAT GCATGCTGGC 
    TCCAGTGACT TACTGCCTGA GGCAATTCCA GAGTCACTGA AAAATATGCT TCTCGTAATG 
    GACACTGCCG GAATATTCCA CAGTGCAGAC TCGCGGACGG GCTACTCTGA TTTATGGGAG 
    ATCACGTGGG AAAGGATAGA TTGCTTTTTA CCTCGTCTAA GGGAAGAACT GTTTAAACAA 
    ACTGTGATAC AAGATCCACC CAATATAGTT ACAACAGAAA CTCCTGTTGA GCCAAGACCT 
    CCCCCTCAAT CAACTCAGTC AGAGAGACCA GCGGAGCAGG GACCTTGTGA CTCAGAGACT 
    CAAAGTTTAG CAATCTCATC TGCTCCAGTA GTTGGATCAA GTTCTTCCTG CACCCCAGTT 
    AGAGTCAGCC CTGTAACAGA TATACCTCCA CCTATAACTC AGTCTCCAGT CATCCTGCAT 
    CCTCTGACCT CCCCGCTGCA GGTGGGAGTG CCACCCATGT CGCTGCCAAT CATCCTCAAC 
    CCTGCATTAA TTGAAGCTAC CTCTCCAGTT CCGCTCTTGT CCACTCCCCG ACCTGCTGAT 
    GTATCATCTG TCTCAGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761943.1
    CDS100..5766
    Translation

    Target ORF information:

    RefSeq Version XM_031906083.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031906083.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGGTACAAAT CTCAGGAGAA AAGGGTAAAG 
    AACCACAAAT ATGACAGTAA ATCCAACCGC TTCAAAAAGA AAACCAAGGA AAAAGAAAAT 
    TCTGTCCGCC GTACACGGGC TTCAAATCAG CGACAATACC GATCTCACAG TGATGATGAG 
    GAAGATGAAA GTGTACCAGC CAGCTATCAC ACTGTGTCAT TACAGGTTAG TCATGATCTT 
    TTGGACCTAA TGCACACACT TCACACAAGA GCTGCCACCA TATACAGTTC TTGGGCAGAG 
    GAACAGCGCA ACCTAGAGAC AGCTGCTGAG AAAATTACAG CCGATTCTAA GACACTGTGG 
    TCAAACTGTT GGTGTCCTTT ACTGCAAGGT ATTGCTTGGC TCTGCTGTGA TGCTCGGCGC 
    CAGGTCAGAA TGCAGGCTCT CACCTACCTG CAAAGGGCAC TTTTAGTCCA TGACTTACAG 
    GCTCTTGATG CACTTGAATG GGAATCATGC TTTAATAAGG TGCTATTTCC ATTGCTGACC 
    AAACTATTGG AAAATATTAG TCCAGCTGAT GTTGGCGGGA TGGAGGAGAC CAGAATGAGA 
    GGCTCCACTC TGCTCTCAAA GGTATTTCTA CAGCATCTGT CTCCTCTTCT TTCTCTGTCG 
    ACATTTGCTG CCTTGTGGCT TACTATTCTT GATTTCATGG ACAAGTACAT GCATGCTGGC 
    TCCAGTGACT TACTGCCTGA GGCAATTCCA GAGTCACTGA AAAATATGCT TCTCGTAATG 
    GACACTGCCG GAATATTCCA CAGTGCAGAC TCGCGGACGG GCTACTCTGA TTTATGGGAG 
    ATCACGTGGG AAAGGATAGA TTGCTTTTTA CCTCGTCTAA GGGAAGAACT GTTTAAACAA 
    ACTGTGATAC AAGATCCACC CAATATAGTT ACAACAGAAA CTCCTGTTGA GCCAAGACCT 
    CCCCCTCAAT CAACTCAGTC AGAGAGACCA GCGGAGCAGG GACCTTGTGA CTCAGAGACT 
    CAAAGTTTAG CAATCTCATC TGCTCCAGTA GTTGGATCAA GTTCTTCCTG CACCCCAGTT 
    AGAGTCAGCC CTGTAACAGA TATACCTCCA CCTATAACTC AGTCTCCAGT CATCCTGCAT 
    CCTCTGACCT CCCCGCTGCA GGTGGGAGTG CCACCCATGT CGCTGCCAAT CATCCTCAAC 
    CCTGCATTAA TTGAAGCTAC CTCTCCAGTT CCGCTCTTGT CCACTCCCCG ACCTGCTGAT 
    GTATCATCTG TCTCAGAGGT CAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_031906084.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761944.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OXa62536
    Clone ID Related Accession (Same CDS sequence) XM_031906084.1
    Accession Version XM_031906084.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5661bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG 
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA 
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT 
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GCTTTTGGAC 
    CTAATGCACA CACTTCACAC AAGAGCTGCC ACCATATACA GTTCTTGGGC AGAGGAACAG 
    CGCAACCTAG AGACAGCTGC TGAGAAAATT ACAGCCGATT CTAAGACACT GTGGTCAAAC 
    TGTTGGTGTC CTTTACTGCA AGGTATTGCT TGGCTCTGCT GTGATGCTCG GCGCCAGGTC 
    AGAATGCAGG CTCTCACCTA CCTGCAAAGG GCACTTTTAG TCCATGACTT ACAGGCTCTT 
    GATGCACTTG AATGGGAATC ATGCTTTAAT AAGGTGCTAT TTCCATTGCT GACCAAACTA 
    TTGGAAAATA TTAGTCCAGC TGATGTTGGC GGGATGGAGG AGACCAGAAT GAGAGGCTCC 
    ACTCTGCTCT CAAAGGTATT TCTACAGCAT CTGTCTCCTC TTCTTTCTCT GTCGACATTT 
    GCTGCCTTGT GGCTTACTAT TCTTGATTTC ATGGACAAGT ACATGCATGC TGGCTCCAGT 
    GACTTACTGC CTGAGGCAAT TCCAGAGTCA CTGAAAAATA TGCTTCTCGT AATGGACACT 
    GCCGGAATAT TCCACAGTGC AGACTCGCGG ACGGGCTACT CTGATTTATG GGAGATCACG 
    TGGGAAAGGA TAGATTGCTT TTTACCTCGT CTAAGGGAAG AACTGTTTAA ACAAACTGTG 
    ATACAAGATC CACCCAATAT AGTTACAACA GAAACTCCTG TTGAGCCAAG ACCTCCCCCT 
    CAATCAACTC AGTCAGAGAG ACCAGCGGAG CAGGGACCTT GTGACTCAGA GACTCAAAGT 
    TTAGCAATCT CATCTGCTCC AGTAGTTGGA TCAAGTTCTT CCTGCACCCC AGTTAGAGTC 
    AGCCCTGTAA CAGATATACC TCCACCTATA ACTCAGTCTC CAGTCATCCT GCATCCTCTG 
    ACCTCCCCGC TGCAGGTGGG AGTGCCACCC ATGTCGCTGC CAATCATCCT CAACCCTGCA 
    TTAATTGAAG CTACCTCTCC AGTTCCGCTC TTGTCCACTC CCCGACCTGC TGATGTATCA 
    TCTGTCTCAG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761944.1
    CDS100..5760
    Translation

    Target ORF information:

    RefSeq Version XM_031906084.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031906084.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGTGTGTGGT ACAAATCTCA GGAGAAAAGG 
    GTAAAGAACC ACAAATATGA CAGTAAATCC AACCGCTTCA AAAAGAAAAC CAAGGAAAAA 
    GAAAATTCTG TCCGCCGTAC ACGGGCTTCA AATCAGCGAC AATACCGATC TCACAGTGAT 
    GATGAGGAAG ATGAAAGTGT ACCAGCCAGC TATCACACTG TGTCATTACA GCTTTTGGAC 
    CTAATGCACA CACTTCACAC AAGAGCTGCC ACCATATACA GTTCTTGGGC AGAGGAACAG 
    CGCAACCTAG AGACAGCTGC TGAGAAAATT ACAGCCGATT CTAAGACACT GTGGTCAAAC 
    TGTTGGTGTC CTTTACTGCA AGGTATTGCT TGGCTCTGCT GTGATGCTCG GCGCCAGGTC 
    AGAATGCAGG CTCTCACCTA CCTGCAAAGG GCACTTTTAG TCCATGACTT ACAGGCTCTT 
    GATGCACTTG AATGGGAATC ATGCTTTAAT AAGGTGCTAT TTCCATTGCT GACCAAACTA 
    TTGGAAAATA TTAGTCCAGC TGATGTTGGC GGGATGGAGG AGACCAGAAT GAGAGGCTCC 
    ACTCTGCTCT CAAAGGTATT TCTACAGCAT CTGTCTCCTC TTCTTTCTCT GTCGACATTT 
    GCTGCCTTGT GGCTTACTAT TCTTGATTTC ATGGACAAGT ACATGCATGC TGGCTCCAGT 
    GACTTACTGC CTGAGGCAAT TCCAGAGTCA CTGAAAAATA TGCTTCTCGT AATGGACACT 
    GCCGGAATAT TCCACAGTGC AGACTCGCGG ACGGGCTACT CTGATTTATG GGAGATCACG 
    TGGGAAAGGA TAGATTGCTT TTTACCTCGT CTAAGGGAAG AACTGTTTAA ACAAACTGTG 
    ATACAAGATC CACCCAATAT AGTTACAACA GAAACTCCTG TTGAGCCAAG ACCTCCCCCT 
    CAATCAACTC AGTCAGAGAG ACCAGCGGAG CAGGGACCTT GTGACTCAGA GACTCAAAGT 
    TTAGCAATCT CATCTGCTCC AGTAGTTGGA TCAAGTTCTT CCTGCACCCC AGTTAGAGTC 
    AGCCCTGTAA CAGATATACC TCCACCTATA ACTCAGTCTC CAGTCATCCT GCATCCTCTG 
    ACCTCCCCGC TGCAGGTGGG AGTGCCACCC ATGTCGCTGC CAATCATCCT CAACCCTGCA 
    TTAATTGAAG CTACCTCTCC AGTTCCGCTC TTGTCCACTC CCCGACCTGC TGATGTATCA 
    TCTGTCTCAG AGGTCAACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_031906085.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761945.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OXa62537
    Clone ID Related Accession (Same CDS sequence) XM_031906085.1
    Accession Version XM_031906085.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5655bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGGTACAAAT CTCAGGAGAA AAGGGTAAAG 
    AACCACAAAT ATGACAGTAA ATCCAACCGC TTCAAAAAGA AAACCAAGGA AAAAGAAAAT 
    TCTGTCCGCC GTACACGGGC TTCAAATCAG CGACAATACC GATCTCACAG TGATGATGAG 
    GAAGATGAAA GTGTACCAGC CAGCTATCAC ACTGTGTCAT TACAGCTTTT GGACCTAATG 
    CACACACTTC ACACAAGAGC TGCCACCATA TACAGTTCTT GGGCAGAGGA ACAGCGCAAC 
    CTAGAGACAG CTGCTGAGAA AATTACAGCC GATTCTAAGA CACTGTGGTC AAACTGTTGG 
    TGTCCTTTAC TGCAAGGTAT TGCTTGGCTC TGCTGTGATG CTCGGCGCCA GGTCAGAATG 
    CAGGCTCTCA CCTACCTGCA AAGGGCACTT TTAGTCCATG ACTTACAGGC TCTTGATGCA 
    CTTGAATGGG AATCATGCTT TAATAAGGTG CTATTTCCAT TGCTGACCAA ACTATTGGAA 
    AATATTAGTC CAGCTGATGT TGGCGGGATG GAGGAGACCA GAATGAGAGG CTCCACTCTG 
    CTCTCAAAGG TATTTCTACA GCATCTGTCT CCTCTTCTTT CTCTGTCGAC ATTTGCTGCC 
    TTGTGGCTTA CTATTCTTGA TTTCATGGAC AAGTACATGC ATGCTGGCTC CAGTGACTTA 
    CTGCCTGAGG CAATTCCAGA GTCACTGAAA AATATGCTTC TCGTAATGGA CACTGCCGGA 
    ATATTCCACA GTGCAGACTC GCGGACGGGC TACTCTGATT TATGGGAGAT CACGTGGGAA 
    AGGATAGATT GCTTTTTACC TCGTCTAAGG GAAGAACTGT TTAAACAAAC TGTGATACAA 
    GATCCACCCA ATATAGTTAC AACAGAAACT CCTGTTGAGC CAAGACCTCC CCCTCAATCA 
    ACTCAGTCAG AGAGACCAGC GGAGCAGGGA CCTTGTGACT CAGAGACTCA AAGTTTAGCA 
    ATCTCATCTG CTCCAGTAGT TGGATCAAGT TCTTCCTGCA CCCCAGTTAG AGTCAGCCCT 
    GTAACAGATA TACCTCCACC TATAACTCAG TCTCCAGTCA TCCTGCATCC TCTGACCTCC 
    CCGCTGCAGG TGGGAGTGCC ACCCATGTCG CTGCCAATCA TCCTCAACCC TGCATTAATT 
    GAAGCTACCT CTCCAGTTCC GCTCTTGTCC ACTCCCCGAC CTGCTGATGT ATCATCTGTC 
    TCAGAGGTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761945.1
    CDS100..5754
    Translation

    Target ORF information:

    RefSeq Version XM_031906085.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031906085.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGA TCTCTCCATG TCTACTCTGC 
    AAAATGGAGC TTAATAGTGG TGAACAGTCC AAAAACCTGA ACCAGTTAGA GAGGGTACTT 
    CTTTTTAAGA GCCTGAAGTT GAAAATGAGA GCTGGAGGAA TGAGTGACTC CTCAAAATGG 
    AAAAAGCAGA AGAAATCTCC TAGGCAGCCA CGTCATGTTA CCAAAGCTAT GGCGGCAGCA 
    GAACCCTCCT CTCAGCAACC AGTAGTTAAT GGCACCACAA TTACTACTAA CCTCACTGGT 
    GAAGTTCCTT TTATTGATGC TTCCTCTCCA GATGCTAATT TTCCCATTTC AGGGGTGAGT 
    TCAGAGGCCA CGTCTGCTGT AGTCAGTCCT ACCACAGATA GTGGTGTAGA TATGTCCTCT 
    CAGGCAACAT CTAAAGAAGA CCTTTCTGAA CTGGATCCTG CAAGTCCATC TCTTTCTGCC 
    ATTCAGCAAT CAGGAGACAT TTGTCTTTCT GAACAGCAAA ACCAGGATGA TGATAAGATT 
    GAAGACCTTA TGGAAAGGCA TACATATTCT GCTTCTGTGG AATCCATACC AGAAGTATTA 
    GATGATAATG TGTCATTGGC TGATCAATCA GACTCCGCTT CAGTCCATGA TATGGATTAT 
    GTAAATCCTA GAGGTGTGAG ATTTACCCAA TCTTCACAGA AAGAAGGAAC AGTCTTAGTA 
    CCATATGGAC TGCCATGCAT TCGGGAATTA TTTCGTTTTC TTATTTCTTT AACCAACCCC 
    CATGATCGAC ATAACTCCGA AGTGATGATA CACATGGGTT TACAGTTGAT CACAGTTGCT 
    CTTGAGTCTG CACCTATTGA AAATTATGTT TCGCTTTTAG GCCTTGTAAA GGATGAACTT 
    TGTCGACACT TATTTCAGCT ACTGAGCATT GAGAGACTGA ACCTATATGC AGCTTCCTTG 
    CGAGCATGTT TTCTTTTGTT TGAGGGTATG AGGGAGCATT TGAAGTTCCA GCTAGAGATG 
    TACATAAAGA AGCTCATGGA TATTATTACT GTGGAGAACC CTAAGATGCC TTATGAGATG 
    AAAGAAATGG CTCTTGAAGC TATTGTGCAA TTGTGGAGAA TCCCGAGCTT TGTTACAGAA 
    TTATACATTA ATTACGACTG TGATTATTAT TGCTCCAACC TTTTTGAAGA TCTCACTAAA 
    CTGCTTTCCA AGAATGCCTT TCCAGTTTCT GGACAGCTGT ACACCACTCA TCTCCTTTCC 
    CTGGAAGCTC TGCTAACTGT CATTGATAGC ACAGAATCAC ACTGCCAGGC TAAAAATCTA 
    AGCAATGTGT CACAGGATAA GAAAGAAACT GGAAAACCAA GTGGAGATCT GTCAGAAGGG 
    ACCAAAGATT CTGGAAATCT CAATGAACAC TCCATTGAAG TTAAGCCATT CACTGTTAGT 
    GCAGAAAACA ATGGATCGAA GCCCCCCACA AGTGGCCACT TAATGGCAGA TAAAATGACT 
    CTTGGGGTCC AAGAAACAGA GTACAGCACT GATGGAGGTG AGAAGAAGAC TTTAAAGAAG 
    CCCCAACGCT TTTCCTGCTT ACTTCCGAAT GTTCAAGAGC TGAATGACAT TAAGACCAAA 
    AAGAAGCTTC TTATAACTGG AACTGAGCAG TTCAACCAGA AACCAAAGAA AGGAGTGCAG 
    TTTCTTCAGG AAAAAGACCT TTTGGCAACC CCCATGGATA ATGCTGAGGT AGCACAGTGG 
    CTCAGAGAAA ATCCAAGGCT GGACAAGAAA ATGATTGGGG AGTTTGTGAG TGATCGCAAA 
    AATCTGGACT TACTTGAAAG TTTTGTAGGA ACCTTCCATT TCCAAGGCCT GAGAGTTGAT 
    GAAGCTTTAA GGCTTTACCT TGAAGCTTTT CGTTTGCCTG GCGAGGCACC TGTGATTCAG 
    AGGTTACTGG AGGCTTTTAC AGAGCACTGG CGAAAATCCA ATGGAACTCC TTTTGCACAC 
    AGTGATGCCT GCTTTGCTTT GGCTTATGCA GTTATCATGC TGAACACAGA CCAACACAAT 
    CACAATGTCC GCAAGCAAAA TGTGCCAATG ACTTTAGAGG AATTCAGGAA AAATTTAAAG 
    GGTGTTAATG GTGGAAAAGA TTTTGACCAA GATATGCTAG AGGATATTTA TCACGCAATC 
    AAAAATGAAG AAATTGTAAT GCCTGAAGAG CAGACTGGCT TAGTAAAAGA GAATTATTTT 
    TGGAATGTTT TGCTGCATCG GGGAGCCACT CCAGAAGGAA TGTTCTTGCA TGTGGATCCT 
    GGAAGTTATG ACCATGACCT CTTTACTATG ACTTGGGGGC CAACAATAGC TGCCTTGTCC 
    TATGTGTTTG ATAAGAGCAT GGATGAAACG ATTATTCAGA AAGCAATATC TGGTTTTAGG 
    AAATGTGCAA TGATATCTGC TCACTATGGT CTCAGTGATG TTTTTGACAA TCTCATAATT 
    TCCTTGTGCA AGTTTACAAC ACTTAGCAGT GAAGCCGTTG AAAATCTTCC CACTGTTTTT 
    GGAAGCAACC TAAAAGCTCA GATCGCTGCA AAGACTGTGT TCCACTTATC CCACAGACAT 
    GGTGACATCC TTCGAGAGGG ATGGAAAAAT ATTATGGACT CCATGCTGCA GCTTTTTAGA 
    GCTGAGCTGC TTCCCAAGGC TATGGTTGAG GTTGAAGATT TTGTAGACCC CAATGGAAAA 
    ATATCTCTTC AACGTGAGGA AATCCCTGCC AACCGTGGGG AATCCACTGT ACTAAGTTTT 
    GTCAGCTGGC TGACTCTGAG TGGGACAGAA CAGTCAAGTC TAAGAGGACC TTCTACTGAA 
    AATCAAGAGG CCAAGAAACT AGCGCTGGAA TGTATCAAGT TATGTGATCC AGAGAAACTG 
    ATCACAGAGA GCAAGTTTCT TCAACTGGAG TCTTTGCAAG AACTAATGAA GGCTCTGATA 
    TCTGTGACAC CAGATGAAGA AACGTATGAT GAAGAAGATG CTGCATTCTG CCTAGAAATG 
    TTAATGAGAA TTGTGCTAGA AAACAGAGAT CGTGTTGGCT GTGTTTGGCA GGCTGTACGA 
    GATCATCTCT ATCGTCTATG TGTCCATGCT GTAGAGTTCT GCTTCCTGGT TGAAAGAGCA 
    GTTGTAGGGC TGCTGCGCTT GGCAATACGC CTTTTACGCA GAGAGGAAAT TAGTGGTCAG 
    GTTATCCTCT CCTTGAGAAT TCTTTTAATG ATGAAACCAA CTGTGTTGTC TAAAGTGAGT 
    CGCCAAGTTG CTTTTGGTGT GCATGAGCTC TTGAAAACCA ATGCTGCTAA CATCCATTCC 
    AGCAATGACT GGTACACACT ATTCTGCCTT TTGGAGTGCA TTGGGGCAGG AGTGAAGCCG 
    CCCCCTGCCT TACAGGTGAC GACGAGAGCT GACAATGACA CAGGTGCGCA GTCTGACAGT 
    GAAGTTTCCT CATACCATCC AAGTGAAGTG AATCTTGATC GTGGATACAC CTCAGACTCT 
    GAGGTATATG CAGAACACAG CAAGCAGTCA AAAATGCACC GCTCTGTGAC TGATGTGGAT 
    ATGATGAACA GTGGCTGGCT GATGGTTGGA AAGGAAGATG TTGACTCTTC TAAAGTTGCT 
    ACAGTTATTA ACAAACCTGT TGCTAATGCC CCAGTGAATC AATACAGCCT AACCATTGGT 
    TTGGATTTTG GCCCTCACGA TACCAAATCT CTCATGAAGA GCGTGGAGTC CCTTTCTTTT 
    ATTGTTCGAG ATGCTGCACA TGTCACACCA GAAAACTTTG AACTCTGTGT TAAAACAATC 
    CGTGTCTTTG TAGAGGCAAG TCTAAATGGA GGGTACAAAT CTCAGGAGAA AAGGGTAAAG 
    AACCACAAAT ATGACAGTAA ATCCAACCGC TTCAAAAAGA AAACCAAGGA AAAAGAAAAT 
    TCTGTCCGCC GTACACGGGC TTCAAATCAG CGACAATACC GATCTCACAG TGATGATGAG 
    GAAGATGAAA GTGTACCAGC CAGCTATCAC ACTGTGTCAT TACAGCTTTT GGACCTAATG 
    CACACACTTC ACACAAGAGC TGCCACCATA TACAGTTCTT GGGCAGAGGA ACAGCGCAAC 
    CTAGAGACAG CTGCTGAGAA AATTACAGCC GATTCTAAGA CACTGTGGTC AAACTGTTGG 
    TGTCCTTTAC TGCAAGGTAT TGCTTGGCTC TGCTGTGATG CTCGGCGCCA GGTCAGAATG 
    CAGGCTCTCA CCTACCTGCA AAGGGCACTT TTAGTCCATG ACTTACAGGC TCTTGATGCA 
    CTTGAATGGG AATCATGCTT TAATAAGGTG CTATTTCCAT TGCTGACCAA ACTATTGGAA 
    AATATTAGTC CAGCTGATGT TGGCGGGATG GAGGAGACCA GAATGAGAGG CTCCACTCTG 
    CTCTCAAAGG TATTTCTACA GCATCTGTCT CCTCTTCTTT CTCTGTCGAC ATTTGCTGCC 
    TTGTGGCTTA CTATTCTTGA TTTCATGGAC AAGTACATGC ATGCTGGCTC CAGTGACTTA 
    CTGCCTGAGG CAATTCCAGA GTCACTGAAA AATATGCTTC TCGTAATGGA CACTGCCGGA 
    ATATTCCACA GTGCAGACTC GCGGACGGGC TACTCTGATT TATGGGAGAT CACGTGGGAA 
    AGGATAGATT GCTTTTTACC TCGTCTAAGG GAAGAACTGT TTAAACAAAC TGTGATACAA 
    GATCCACCCA ATATAGTTAC AACAGAAACT CCTGTTGAGC CAAGACCTCC CCCTCAATCA 
    ACTCAGTCAG AGAGACCAGC GGAGCAGGGA CCTTGTGACT CAGAGACTCA AAGTTTAGCA 
    ATCTCATCTG CTCCAGTAGT TGGATCAAGT TCTTCCTGCA CCCCAGTTAG AGTCAGCCCT 
    GTAACAGATA TACCTCCACC TATAACTCAG TCTCCAGTCA TCCTGCATCC TCTGACCTCC 
    CCGCTGCAGG TGGGAGTGCC ACCCATGTCG CTGCCAATCA TCCTCAACCC TGCATTAATT 
    GAAGCTACCT CTCCAGTTCC GCTCTTGTCC ACTCCCCGAC CTGCTGATGT ATCATCTGTC 
    TCAGAGGTCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_012966851.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822305.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OXa62538
    Clone ID Related Accession (Same CDS sequence) XM_012966851.3 , XM_012966852.3
    Accession Version XM_012966851.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966851.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822305.1
    CDS262..5835
    Translation

    Target ORF information:

    RefSeq Version XM_012966851.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012966851.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_012966852.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822306.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OXa62538
    Clone ID Related Accession (Same CDS sequence) XM_012966851.3 , XM_012966852.3
    Accession Version XM_012966852.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822306.1
    CDS100..5673
    Translation

    Target ORF information:

    RefSeq Version XM_012966852.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012966852.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGTGTGTGG 
    TACAAATCTC AGGAGAAAAG GGTAAAGAAC CACAAATATG ACAGTAAATC CAACCGCTTC 
    AAAAAGAAAA CCAAGGAAAA AGAAAATTCT GTCCGCCGTA CACGGGCTTC AAATCAGCGA 
    CAATACCGAT CTCACAGTGA TGATGAGGAA GATGAAAGTG TACCAGCCAG CTATCACACT 
    GTGTCATTAC AGGTTAGTCA TGATCTTTTG GACCTAATGC ACACACTTCA CACAAGAGCT 
    GCCACCATAT ACAGTTCTTG GGCAGAGGAA CAGCGCAACC TAGAGACAGC TGCTGAGAAA 
    ATTACAGCCG ATTCTAAGAC ACTGTGGTCA AACTGTTGGT GTCCTTTACT GCAAGGTATT 
    GCTTGGCTCT GCTGTGATGC TCGGCGCCAG GTCAGAATGC AGGCTCTCAC CTACCTGCAA 
    AGGGCACTTT TAGTCCATGA CTTACAGGCT CTTGATGCAC TTGAATGGGA ATCATGCTTT 
    AATAAGGTGC TATTTCCATT GCTGACCAAA CTATTGGAAA ATATTAGTCC AGCTGATGTT 
    GGCGGGATGG AGGAGACCAG AATGAGAGGC TCCACTCTGC TCTCAAAGGT ATTTCTACAG 
    CATCTGTCTC CTCTTCTTTC TCTGTCGACA TTTGCTGCCT TGTGGCTTAC TATTCTTGAT 
    TTCATGGACA AGTACATGCA TGCTGGCTCC AGTGACTTAC TGCCTGAGGC AATTCCAGAG 
    TCACTGAAAA ATATGCTTCT CGTAATGGAC ACTGCCGGAA TATTCCACAG TGCAGACTCG 
    CGGACGGGCT ACTCTGATTT ATGGGAGATC ACGTGGGAAA GGATAGATTG CTTTTTACCT 
    CGTCTAAGGG AAGAACTGTT TAAACAAACT GTGATACAAG ATCCACCCAA TATAGTTACA 
    ACAGAAACTC CTGTTGAGCC AAGACCTCCC CCTCAATCAA CTCAGTCAGA GAGACCAGCG 
    GAGCAGGGAC CTTGTGACTC AGAGACTCAA AGTTTAGCAA TCTCATCTGC TCCAGTAGTT 
    GGATCAAGTT CTTCCTGCAC CCCAGTTAGA GTCAGCCCTG TAACAGATAT ACCTCCACCT 
    ATAACTCAGT CTCCAGTCAT CCTGCATCCT CTGACCTCCC CGCTGCAGGT GGGAGTGCCA 
    CCCATGTCGC TGCCAATCAT CCTCAACCCT GCATTAATTG AAGCTACCTC TCCAGTTCCG 
    CTCTTGTCCA CTCCCCGACC TGCTGATGTA TCATCTGTCT CAGAGGTCAA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_031906086.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761946.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OXa62539
    Clone ID Related Accession (Same CDS sequence) XM_031906086.1
    Accession Version XM_031906086.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4989bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCATTTT TCCTTGAGGC TTACAATATC TTTTGGAAAA ACAAACGTGT TCAGTTGAAA 
    ATGAGAGCTG GAGGAATGAG TGACTCCTCA AAATGGAAAA AGCAGAAGAA ATCTCCTAGG 
    CAGCCACGTC ATGTTACCAA AGCTATGGCG GCAGCAGAAC CCTCCTCTCA GCAACCAGTA 
    GTTAATGGCA CCACAATTAC TACTAACCTC ACTGGTGAAG TTCCTTTTAT TGATGCTTCC 
    TCTCCAGATG CTAATTTTCC CATTTCAGGG GTGAGTTCAG AGGCCACGTC TGCTGTAGTC 
    AGTCCTACCA CAGATAGTGG TGTAGATATG TCCTCTCAGG CAACATCTAA AGAAGACCTT 
    TCTGAACTGG ATCCTGCAAG TCCATCTCTT TCTGCCATTC AGCAATCAGG AGACATTTGT 
    CTTTCTGAAC AGCAAAACCA GGATGATGAT AAGATTGAAG ACCTTATGGA AAGGCATACA 
    TATTCTGCTT CTGTGGAATC CATACCAGAA GTATTAGATG ATAATGTGTC ATTGGCTGAT 
    CAATCAGACT CCGCTTCAGT CCATGATATG GATTATGTAA ATCCTAGAGG TGTGAGATTT 
    ACCCAATCTT CACAGAAAGA AGGAACAGTC TTAGTACCAT ATGGACTGCC ATGCATTCGG 
    GAATTATTTC GTTTTCTTAT TTCTTTAACC AACCCCCATG ATCGACATAA CTCCGAAGTG 
    ATGATACACA TGGGTTTACA GTTGATCACA GTTGCTCTTG AGTCTGCACC TATTGAAAAT 
    TATGTTTCGC TTTTAGGCCT TGTAAAGGAT GAACTTTGTC GACACTTATT TCAGCTACTG 
    AGCATTGAGA GACTGAACCT ATATGCAGCT TCCTTGCGAG CATGTTTTCT TTTGTTTGAG 
    GGTATGAGGG AGCATTTGAA GTTCCAGCTA GAGATGTACA TAAAGAAGCT CATGGATATT 
    ATTACTGTGG AGAACCCTAA GATGCCTTAT GAGATGAAAG AAATGGCTCT TGAAGCTATT 
    GTGCAATTGT GGAGAATCCC GAGCTTTGTT ACAGAATTAT ACATTAATTA CGACTGTGAT 
    TATTATTGCT CCAACCTTTT TGAAGATCTC ACTAAACTGC TTTCCAAGAA TGCCTTTCCA 
    GTTTCTGGAC AGCTGTACAC CACTCATCTC CTTTCCCTGG AAGCTCTGCT AACTGTCATT 
    GATAGCACAG AATCACACTG CCAGGCTAAA AATCTAAGCA ATGTGTCACA GGATAAGAAA 
    GAAACTGGAA AACCAAGTGG AGATCTGTCA GAAGGGACCA AAGATTCTGG AAATCTCAAT 
    GAACACTCCA TTGAAGTTAA GCCATTCACT GTTAGTGCAG AAAACAATGG ATCGAAGCCC 
    CCCACAAGTG GCCACTTAAT GGCAGATAAA ATGACTCTTG GGGTCCAAGA AACAGAGTAC 
    AGCACTGATG GAGGTGAGAA GAAGACTTTA AAGAAGCCCC AACGCTTTTC CTGCTTACTT 
    CCGAATGTTC AAGAGCTGAA TGACATTAAG ACCAAAAAGA AGCTTCTTAT AACTGGAACT 
    GAGCAGTTCA ACCAGAAACC AAAGAAAGGA GTGCAGTTTC TTCAGGAAAA AGACCTTTTG 
    GCAACCCCCA TGGATAATGC TGAGGTAGCA CAGTGGCTCA GAGAAAATCC AAGGCTGGAC 
    AAGAAAATGA TTGGGGAGTT TGTGAGTGAT CGCAAAAATC TGGACTTACT TGAAAGTTTT 
    GTAGGAACCT TCCATTTCCA AGGCCTGAGA GTTGATGAAG CTTTAAGGCT TTACCTTGAA 
    GCTTTTCGTT TGCCTGGCGA GGCACCTGTG ATTCAGAGGT TACTGGAGGC TTTTACAGAG 
    CACTGGCGAA AATCCAATGG AACTCCTTTT GCACACAGTG ATGCCTGCTT TGCTTTGGCT 
    TATGCAGTTA TCATGCTGAA CACAGACCAA CACAATCACA ATGTCCGCAA GCAAAATGTG 
    CCAATGACTT TAGAGGAATT CAGGAAAAAT TTAAAGGGTG TTAATGGTGG AAAAGATTTT 
    GACCAAGATA TGCTAGAGGA TATTTATCAC GCAATCAAAA ATGAAGAAAT TGTAATGCCT 
    GAAGAGCAGA CTGGCTTAGT AAAAGAGAAT TATTTTTGGA ATGTTTTGCT GCATCGGGGA 
    GCCACTCCAG AAGGAATGTT CTTGCATGTG GATCCTGGAA GTTATGACCA TGACCTCTTT 
    ACTATGACTT GGGGGCCAAC AATAGCTGCC TTGTCCTATG TGTTTGATAA GAGCATGGAT 
    GAAACGATTA TTCAGAAAGC AATATCTGGT TTTAGGAAAT GTGCAATGAT ATCTGCTCAC 
    TATGGTCTCA GTGATGTTTT TGACAATCTC ATAATTTCCT TGTGCAAGTT TACAACACTT 
    AGCAGTGAAG CCGTTGAAAA TCTTCCCACT GTTTTTGGAA GCAACCTAAA AGCTCAGATC 
    GCTGCAAAGA CTGTGTTCCA CTTATCCCAC AGACATGGTG ACATCCTTCG AGAGGGATGG 
    AAAAATATTA TGGACTCCAT GCTGCAGCTT TTTAGAGCTG AGCTGCTTCC CAAGGCTATG 
    GTTGAGGTTG AAGATTTTGT AGACCCCAAT GGAAAAATAT CTCTTCAACG TGAGGAAATC 
    CCTGCCAACC GTGGGGAATC CACTGTACTA AGTTTTGTCA GCTGGCTGAC TCTGAGTGGG 
    ACAGAACAGT CAAGTCTAAG AGGACCTTCT ACTGAAAATC AAGAGGCCAA GAAACTAGCG 
    CTGGAATGTA TCAAGTTATG TGATCCAGAG AAACTGATCA CAGAGAGCAA GTTTCTTCAA 
    CTGGAGTCTT TGCAAGAACT AATGAAGGCT CTGATATCTG TGACACCAGA TGAAGAAACG 
    TATGATGAAG AAGATGCTGC ATTCTGCCTA GAAATGTTAA TGAGAATTGT GCTAGAAAAC 
    AGAGATCGTG TTGGCTGTGT TTGGCAGGCT GTACGAGATC ATCTCTATCG TCTATGTGTC 
    CATGCTGTAG AGTTCTGCTT CCTGGTTGAA AGAGCAGTTG TAGGGCTGCT GCGCTTGGCA 
    ATACGCCTTT TACGCAGAGA GGAAATTAGT GGTCAGGTTA TCCTCTCCTT GAGAATTCTT 
    TTAATGATGA AACCAACTGT GTTGTCTAAA GTGAGTCGCC AAGTTGCTTT TGGTGTGCAT 
    GAGCTCTTGA AAACCAATGC TGCTAACATC CATTCCAGCA ATGACTGGTA CACACTATTC 
    TGCCTTTTGG AGTGCATTGG GGCAGGAGTG AAGCCGCCCC CTGCCTTACA GGTGACGACG 
    AGAGCTGACA ATGACACAGG TGCGCAGTCT GACAGTGAAG TTTCCTCATA CCATCCAAGT 
    GAAGTGAATC TTGATCGTGG ATACACCTCA GACTCTGAGG TATATGCAGA ACACAGCAAG 
    CAGTCAAAAA TGCACCGCTC TGTGACTGAT GTGGATATGA TGAACAGTGG CTGGCTGATG 
    GTTGGAAAGG AAGATGTTGA CTCTTCTAAA GTTGCTACAG TTATTAACAA ACCTGTTGCT 
    AATGCCCCAG TGAATCAATA CAGCCTAACC ATTGGTTTGG ATTTTGGCCC TCACGATACC 
    AAATCTCTCA TGAAGAGCGT GGAGTCCCTT TCTTTTATTG TTCGAGATGC TGCACATGTC 
    ACACCAGAAA ACTTTGAACT CTGTGTTAAA ACAATCCGTG TCTTTGTAGA GGCAAGTCTA 
    AATGGAGGTG TGTGGTACAA ATCTCAGGAG AAAAGGGTAA AGAACCACAA ATATGACAGT 
    AAATCCAACC GCTTCAAAAA GAAAACCAAG GAAAAAGAAA ATTCTGTCCG CCGTACACGG 
    GCTTCAAATC AGCGACAATA CCGATCTCAC AGTGATGATG AGGAAGATGA AAGTGTACCA 
    GCCAGCTATC ACACTGTGTC ATTACAGGTT AGTCATGATC TTTTGGACCT AATGCACACA 
    CTTCACACAA GAGCTGCCAC CATATACAGT TCTTGGGCAG AGGAACAGCG CAACCTAGAG 
    ACAGCTGCTG AGAAAATTAC AGCCGATTCT AAGACACTGT GGTCAAACTG TTGGTGTCCT 
    TTACTGCAAG GTATTGCTTG GCTCTGCTGT GATGCTCGGC GCCAGGTCAG AATGCAGGCT 
    CTCACCTACC TGCAAAGGGC ACTTTTAGTC CATGACTTAC AGGCTCTTGA TGCACTTGAA 
    TGGGAATCAT GCTTTAATAA GGTGCTATTT CCATTGCTGA CCAAACTATT GGAAAATATT 
    AGTCCAGCTG ATGTTGGCGG GATGGAGGAG ACCAGAATGA GAGGCTCCAC TCTGCTCTCA 
    AAGGTATTTC TACAGCATCT GTCTCCTCTT CTTTCTCTGT CGACATTTGC TGCCTTGTGG 
    CTTACTATTC TTGATTTCAT GGACAAGTAC ATGCATGCTG GCTCCAGTGA CTTACTGCCT 
    GAGGCAATTC CAGAGTCACT GAAAAATATG CTTCTCGTAA TGGACACTGC CGGAATATTC 
    CACAGTGCAG ACTCGCGGAC GGGCTACTCT GATTTATGGG AGATCACGTG GGAAAGGATA 
    GATTGCTTTT TACCTCGTCT AAGGGAAGAA CTGTTTAAAC AAACTGTGAT ACAAGATCCA 
    CCCAATATAG TTACAACAGA AACTCCTGTT GAGCCAAGAC CTCCCCCTCA ATCAACTCAG 
    TCAGAGAGAC CAGCGGAGCA GGGACCTTGT GACTCAGAGA CTCAAAGTTT AGCAATCTCA 
    TCTGCTCCAG TAGTTGGATC AAGTTCTTCC TGCACCCCAG TTAGAGTCAG CCCTGTAACA 
    GATATACCTC CACCTATAAC TCAGTCTCCA GTCATCCTGC ATCCTCTGAC CTCCCCGCTG 
    CAGGTGGGAG TGCCACCCAT GTCGCTGCCA ATCATCCTCA ACCCTGCATT AATTGAAGCT 
    ACCTCTCCAG TTCCGCTCTT GTCCACTCCC CGACCTGCTG ATGTATCATC TGTCTCAGAG 
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761946.1
    CDS16..5004
    Translation

    Target ORF information:

    RefSeq Version XM_031906086.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_031906086.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCCATTTT TCCTTGAGGC TTACAATATC TTTTGGAAAA ACAAACGTGT TCAGTTGAAA 
    ATGAGAGCTG GAGGAATGAG TGACTCCTCA AAATGGAAAA AGCAGAAGAA ATCTCCTAGG 
    CAGCCACGTC ATGTTACCAA AGCTATGGCG GCAGCAGAAC CCTCCTCTCA GCAACCAGTA 
    GTTAATGGCA CCACAATTAC TACTAACCTC ACTGGTGAAG TTCCTTTTAT TGATGCTTCC 
    TCTCCAGATG CTAATTTTCC CATTTCAGGG GTGAGTTCAG AGGCCACGTC TGCTGTAGTC 
    AGTCCTACCA CAGATAGTGG TGTAGATATG TCCTCTCAGG CAACATCTAA AGAAGACCTT 
    TCTGAACTGG ATCCTGCAAG TCCATCTCTT TCTGCCATTC AGCAATCAGG AGACATTTGT 
    CTTTCTGAAC AGCAAAACCA GGATGATGAT AAGATTGAAG ACCTTATGGA AAGGCATACA 
    TATTCTGCTT CTGTGGAATC CATACCAGAA GTATTAGATG ATAATGTGTC ATTGGCTGAT 
    CAATCAGACT CCGCTTCAGT CCATGATATG GATTATGTAA ATCCTAGAGG TGTGAGATTT 
    ACCCAATCTT CACAGAAAGA AGGAACAGTC TTAGTACCAT ATGGACTGCC ATGCATTCGG 
    GAATTATTTC GTTTTCTTAT TTCTTTAACC AACCCCCATG ATCGACATAA CTCCGAAGTG 
    ATGATACACA TGGGTTTACA GTTGATCACA GTTGCTCTTG AGTCTGCACC TATTGAAAAT 
    TATGTTTCGC TTTTAGGCCT TGTAAAGGAT GAACTTTGTC GACACTTATT TCAGCTACTG 
    AGCATTGAGA GACTGAACCT ATATGCAGCT TCCTTGCGAG CATGTTTTCT TTTGTTTGAG 
    GGTATGAGGG AGCATTTGAA GTTCCAGCTA GAGATGTACA TAAAGAAGCT CATGGATATT 
    ATTACTGTGG AGAACCCTAA GATGCCTTAT GAGATGAAAG AAATGGCTCT TGAAGCTATT 
    GTGCAATTGT GGAGAATCCC GAGCTTTGTT ACAGAATTAT ACATTAATTA CGACTGTGAT 
    TATTATTGCT CCAACCTTTT TGAAGATCTC ACTAAACTGC TTTCCAAGAA TGCCTTTCCA 
    GTTTCTGGAC AGCTGTACAC CACTCATCTC CTTTCCCTGG AAGCTCTGCT AACTGTCATT 
    GATAGCACAG AATCACACTG CCAGGCTAAA AATCTAAGCA ATGTGTCACA GGATAAGAAA 
    GAAACTGGAA AACCAAGTGG AGATCTGTCA GAAGGGACCA AAGATTCTGG AAATCTCAAT 
    GAACACTCCA TTGAAGTTAA GCCATTCACT GTTAGTGCAG AAAACAATGG ATCGAAGCCC 
    CCCACAAGTG GCCACTTAAT GGCAGATAAA ATGACTCTTG GGGTCCAAGA AACAGAGTAC 
    AGCACTGATG GAGGTGAGAA GAAGACTTTA AAGAAGCCCC AACGCTTTTC CTGCTTACTT 
    CCGAATGTTC AAGAGCTGAA TGACATTAAG ACCAAAAAGA AGCTTCTTAT AACTGGAACT 
    GAGCAGTTCA ACCAGAAACC AAAGAAAGGA GTGCAGTTTC TTCAGGAAAA AGACCTTTTG 
    GCAACCCCCA TGGATAATGC TGAGGTAGCA CAGTGGCTCA GAGAAAATCC AAGGCTGGAC 
    AAGAAAATGA TTGGGGAGTT TGTGAGTGAT CGCAAAAATC TGGACTTACT TGAAAGTTTT 
    GTAGGAACCT TCCATTTCCA AGGCCTGAGA GTTGATGAAG CTTTAAGGCT TTACCTTGAA 
    GCTTTTCGTT TGCCTGGCGA GGCACCTGTG ATTCAGAGGT TACTGGAGGC TTTTACAGAG 
    CACTGGCGAA AATCCAATGG AACTCCTTTT GCACACAGTG ATGCCTGCTT TGCTTTGGCT 
    TATGCAGTTA TCATGCTGAA CACAGACCAA CACAATCACA ATGTCCGCAA GCAAAATGTG 
    CCAATGACTT TAGAGGAATT CAGGAAAAAT TTAAAGGGTG TTAATGGTGG AAAAGATTTT 
    GACCAAGATA TGCTAGAGGA TATTTATCAC GCAATCAAAA ATGAAGAAAT TGTAATGCCT 
    GAAGAGCAGA CTGGCTTAGT AAAAGAGAAT TATTTTTGGA ATGTTTTGCT GCATCGGGGA 
    GCCACTCCAG AAGGAATGTT CTTGCATGTG GATCCTGGAA GTTATGACCA TGACCTCTTT 
    ACTATGACTT GGGGGCCAAC AATAGCTGCC TTGTCCTATG TGTTTGATAA GAGCATGGAT 
    GAAACGATTA TTCAGAAAGC AATATCTGGT TTTAGGAAAT GTGCAATGAT ATCTGCTCAC 
    TATGGTCTCA GTGATGTTTT TGACAATCTC ATAATTTCCT TGTGCAAGTT TACAACACTT 
    AGCAGTGAAG CCGTTGAAAA TCTTCCCACT GTTTTTGGAA GCAACCTAAA AGCTCAGATC 
    GCTGCAAAGA CTGTGTTCCA CTTATCCCAC AGACATGGTG ACATCCTTCG AGAGGGATGG 
    AAAAATATTA TGGACTCCAT GCTGCAGCTT TTTAGAGCTG AGCTGCTTCC CAAGGCTATG 
    GTTGAGGTTG AAGATTTTGT AGACCCCAAT GGAAAAATAT CTCTTCAACG TGAGGAAATC 
    CCTGCCAACC GTGGGGAATC CACTGTACTA AGTTTTGTCA GCTGGCTGAC TCTGAGTGGG 
    ACAGAACAGT CAAGTCTAAG AGGACCTTCT ACTGAAAATC AAGAGGCCAA GAAACTAGCG 
    CTGGAATGTA TCAAGTTATG TGATCCAGAG AAACTGATCA CAGAGAGCAA GTTTCTTCAA 
    CTGGAGTCTT TGCAAGAACT AATGAAGGCT CTGATATCTG TGACACCAGA TGAAGAAACG 
    TATGATGAAG AAGATGCTGC ATTCTGCCTA GAAATGTTAA TGAGAATTGT GCTAGAAAAC 
    AGAGATCGTG TTGGCTGTGT TTGGCAGGCT GTACGAGATC ATCTCTATCG TCTATGTGTC 
    CATGCTGTAG AGTTCTGCTT CCTGGTTGAA AGAGCAGTTG TAGGGCTGCT GCGCTTGGCA 
    ATACGCCTTT TACGCAGAGA GGAAATTAGT GGTCAGGTTA TCCTCTCCTT GAGAATTCTT 
    TTAATGATGA AACCAACTGT GTTGTCTAAA GTGAGTCGCC AAGTTGCTTT TGGTGTGCAT 
    GAGCTCTTGA AAACCAATGC TGCTAACATC CATTCCAGCA ATGACTGGTA CACACTATTC 
    TGCCTTTTGG AGTGCATTGG GGCAGGAGTG AAGCCGCCCC CTGCCTTACA GGTGACGACG 
    AGAGCTGACA ATGACACAGG TGCGCAGTCT GACAGTGAAG TTTCCTCATA CCATCCAAGT 
    GAAGTGAATC TTGATCGTGG ATACACCTCA GACTCTGAGG TATATGCAGA ACACAGCAAG 
    CAGTCAAAAA TGCACCGCTC TGTGACTGAT GTGGATATGA TGAACAGTGG CTGGCTGATG 
    GTTGGAAAGG AAGATGTTGA CTCTTCTAAA GTTGCTACAG TTATTAACAA ACCTGTTGCT 
    AATGCCCCAG TGAATCAATA CAGCCTAACC ATTGGTTTGG ATTTTGGCCC TCACGATACC 
    AAATCTCTCA TGAAGAGCGT GGAGTCCCTT TCTTTTATTG TTCGAGATGC TGCACATGTC 
    ACACCAGAAA ACTTTGAACT CTGTGTTAAA ACAATCCGTG TCTTTGTAGA GGCAAGTCTA 
    AATGGAGGTG TGTGGTACAA ATCTCAGGAG AAAAGGGTAA AGAACCACAA ATATGACAGT 
    AAATCCAACC GCTTCAAAAA GAAAACCAAG GAAAAAGAAA ATTCTGTCCG CCGTACACGG 
    GCTTCAAATC AGCGACAATA CCGATCTCAC AGTGATGATG AGGAAGATGA AAGTGTACCA 
    GCCAGCTATC ACACTGTGTC ATTACAGGTT AGTCATGATC TTTTGGACCT AATGCACACA 
    CTTCACACAA GAGCTGCCAC CATATACAGT TCTTGGGCAG AGGAACAGCG CAACCTAGAG 
    ACAGCTGCTG AGAAAATTAC AGCCGATTCT AAGACACTGT GGTCAAACTG TTGGTGTCCT 
    TTACTGCAAG GTATTGCTTG GCTCTGCTGT GATGCTCGGC GCCAGGTCAG AATGCAGGCT 
    CTCACCTACC TGCAAAGGGC ACTTTTAGTC CATGACTTAC AGGCTCTTGA TGCACTTGAA 
    TGGGAATCAT GCTTTAATAA GGTGCTATTT CCATTGCTGA CCAAACTATT GGAAAATATT 
    AGTCCAGCTG ATGTTGGCGG GATGGAGGAG ACCAGAATGA GAGGCTCCAC TCTGCTCTCA 
    AAGGTATTTC TACAGCATCT GTCTCCTCTT CTTTCTCTGT CGACATTTGC TGCCTTGTGG 
    CTTACTATTC TTGATTTCAT GGACAAGTAC ATGCATGCTG GCTCCAGTGA CTTACTGCCT 
    GAGGCAATTC CAGAGTCACT GAAAAATATG CTTCTCGTAA TGGACACTGC CGGAATATTC 
    CACAGTGCAG ACTCGCGGAC GGGCTACTCT GATTTATGGG AGATCACGTG GGAAAGGATA 
    GATTGCTTTT TACCTCGTCT AAGGGAAGAA CTGTTTAAAC AAACTGTGAT ACAAGATCCA 
    CCCAATATAG TTACAACAGA AACTCCTGTT GAGCCAAGAC CTCCCCCTCA ATCAACTCAG 
    TCAGAGAGAC CAGCGGAGCA GGGACCTTGT GACTCAGAGA CTCAAAGTTT AGCAATCTCA 
    TCTGCTCCAG TAGTTGGATC AAGTTCTTCC TGCACCCCAG TTAGAGTCAG CCCTGTAACA 
    GATATACCTC CACCTATAAC TCAGTCTCCA GTCATCCTGC ATCCTCTGAC CTCCCCGCTG 
    CAGGTGGGAG TGCCACCCAT GTCGCTGCCA ATCATCCTCA ACCCTGCATT AATTGAAGCT 
    ACCTCTCCAG TTCCGCTCTT GTCCACTCCC CGACCTGCTG ATGTATCATC TGTCTCAGAG 
    GTCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_018095873.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951362.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID OXa62540
    Clone ID Related Accession (Same CDS sequence) XM_018095873.2
    Accession Version XM_018095873.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA 
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG 
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC 
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA 
    TTACAGCTTT TGGACCTAAT GCACACACTT CACACAAGAG CTGCCACCAT ATACAGTTCT 
    TGGGCAGAGG AACAGCGCAA CCTAGAGACA GCTGCTGAGA AAATTACAGC CGATTCTAAG 
    ACACTGTGGT CAAACTGTTG GTGTCCTTTA CTGCAAGGTA TTGCTTGGCT CTGCTGTGAT 
    GCTCGGCGCC AGGTCAGAAT GCAGGCTCTC ACCTACCTGC AAAGGGCACT TTTAGTCCAT 
    GACTTACAGG CTCTTGATGC ACTTGAATGG GAATCATGCT TTAATAAGGT GCTATTTCCA 
    TTGCTGACCA AACTATTGGA AAATATTAGT CCAGCTGATG TTGGCGGGAT GGAGGAGACC 
    AGAATGAGAG GCTCCACTCT GCTCTCAAAG GTATTTCTAC AGCATCTGTC TCCTCTTCTT 
    TCTCTGTCGA CATTTGCTGC CTTGTGGCTT ACTATTCTTG ATTTCATGGA CAAGTACATG 
    CATGCTGGCT CCAGTGACTT ACTGCCTGAG GCAATTCCAG AGTCACTGAA AAATATGCTT 
    CTCGTAATGG ACACTGCCGG AATATTCCAC AGTGCAGACT CGCGGACGGG CTACTCTGAT 
    TTATGGGAGA TCACGTGGGA AAGGATAGAT TGCTTTTTAC CTCGTCTAAG GGAAGAACTG 
    TTTAAACAAA CTGTGATACA AGATCCACCC AATATAGTTA CAACAGAAAC TCCTGTTGAG 
    CCAAGACCTC CCCCTCAATC AACTCAGTCA GAGAGACCAG CGGAGCAGGG ACCTTGTGAC 
    TCAGAGACTC AAAGTTTAGC AATCTCATCT GCTCCAGTAG TTGGATCAAG TTCTTCCTGC 
    ACCCCAGTTA GAGTCAGCCC TGTAACAGAT ATACCTCCAC CTATAACTCA GTCTCCAGTC 
    ATCCTGCATC CTCTGACCTC CCCGCTGCAG GTGGGAGTGC CACCCATGTC GCTGCCAATC 
    ATCCTCAACC CTGCATTAAT TGAAGCTACC TCTCCAGTTC CGCTCTTGTC CACTCCCCGA 
    CCTGCTGATG TATCATCTGT CTCAGAGGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951362.1
    CDS100..5655
    Translation

    Target ORF information:

    RefSeq Version XM_018095873.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_018095873.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA 
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG 
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC 
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA 
    TTACAGCTTT TGGACCTAAT GCACACACTT CACACAAGAG CTGCCACCAT ATACAGTTCT 
    TGGGCAGAGG AACAGCGCAA CCTAGAGACA GCTGCTGAGA AAATTACAGC CGATTCTAAG 
    ACACTGTGGT CAAACTGTTG GTGTCCTTTA CTGCAAGGTA TTGCTTGGCT CTGCTGTGAT 
    GCTCGGCGCC AGGTCAGAAT GCAGGCTCTC ACCTACCTGC AAAGGGCACT TTTAGTCCAT 
    GACTTACAGG CTCTTGATGC ACTTGAATGG GAATCATGCT TTAATAAGGT GCTATTTCCA 
    TTGCTGACCA AACTATTGGA AAATATTAGT CCAGCTGATG TTGGCGGGAT GGAGGAGACC 
    AGAATGAGAG GCTCCACTCT GCTCTCAAAG GTATTTCTAC AGCATCTGTC TCCTCTTCTT 
    TCTCTGTCGA CATTTGCTGC CTTGTGGCTT ACTATTCTTG ATTTCATGGA CAAGTACATG 
    CATGCTGGCT CCAGTGACTT ACTGCCTGAG GCAATTCCAG AGTCACTGAA AAATATGCTT 
    CTCGTAATGG ACACTGCCGG AATATTCCAC AGTGCAGACT CGCGGACGGG CTACTCTGAT 
    TTATGGGAGA TCACGTGGGA AAGGATAGAT TGCTTTTTAC CTCGTCTAAG GGAAGAACTG 
    TTTAAACAAA CTGTGATACA AGATCCACCC AATATAGTTA CAACAGAAAC TCCTGTTGAG 
    CCAAGACCTC CCCCTCAATC AACTCAGTCA GAGAGACCAG CGGAGCAGGG ACCTTGTGAC 
    TCAGAGACTC AAAGTTTAGC AATCTCATCT GCTCCAGTAG TTGGATCAAG TTCTTCCTGC 
    ACCCCAGTTA GAGTCAGCCC TGTAACAGAT ATACCTCCAC CTATAACTCA GTCTCCAGTC 
    ATCCTGCATC CTCTGACCTC CCCGCTGCAG GTGGGAGTGC CACCCATGTC GCTGCCAATC 
    ATCCTCAACC CTGCATTAAT TGAAGCTACC TCTCCAGTTC CGCTCTTGTC CACTCCCCGA 
    CCTGCTGATG TATCATCTGT CTCAGAGGTC AACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    gbf1 ( XM_018095871.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951360.1 Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID OXa62541
    Clone ID Related Accession (Same CDS sequence) XM_018095871.2
    Accession Version XM_018095871.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1576512000000
    Organism Xenopus tropicalis(tropical clawed frog)
    Product Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA 
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG 
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC 
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA 
    TTACAGGTTA GTCATGATCT TTTGGACCTA ATGCACACAC TTCACACAAG AGCTGCCACC 
    ATATACAGTT CTTGGGCAGA GGAACAGCGC AACCTAGAGA CAGCTGCTGA GAAAATTACA 
    GCCGATTCTA AGACACTGTG GTCAAACTGT TGGTGTCCTT TACTGCAAGG TATTGCTTGG 
    CTCTGCTGTG ATGCTCGGCG CCAGGTCAGA ATGCAGGCTC TCACCTACCT GCAAAGGGCA 
    CTTTTAGTCC ATGACTTACA GGCTCTTGAT GCACTTGAAT GGGAATCATG CTTTAATAAG 
    GTGCTATTTC CATTGCTGAC CAAACTATTG GAAAATATTA GTCCAGCTGA TGTTGGCGGG 
    ATGGAGGAGA CCAGAATGAG AGGCTCCACT CTGCTCTCAA AGGTATTTCT ACAGCATCTG 
    TCTCCTCTTC TTTCTCTGTC GACATTTGCT GCCTTGTGGC TTACTATTCT TGATTTCATG 
    GACAAGTACA TGCATGCTGG CTCCAGTGAC TTACTGCCTG AGGCAATTCC AGAGTCACTG 
    AAAAATATGC TTCTCGTAAT GGACACTGCC GGAATATTCC ACAGTGCAGA CTCGCGGACG 
    GGCTACTCTG ATTTATGGGA GATCACGTGG GAAAGGATAG ATTGCTTTTT ACCTCGTCTA 
    AGGGAAGAAC TGTTTAAACA AACTGTGATA CAAGATCCAC CCAATATAGT TACAACAGAA 
    ACTCCTGTTG AGCCAAGACC TCCCCCTCAA TCAACTCAGT CAGAGAGACC AGCGGAGCAG 
    GGACCTTGTG ACTCAGAGAC TCAAAGTTTA GCAATCTCAT CTGCTCCAGT AGTTGGATCA 
    AGTTCTTCCT GCACCCCAGT TAGAGTCAGC CCTGTAACAG ATATACCTCC ACCTATAACT 
    CAGTCTCCAG TCATCCTGCA TCCTCTGACC TCCCCGCTGC AGGTGGGAGT GCCACCCATG 
    TCGCTGCCAA TCATCCTCAA CCCTGCATTA ATTGAAGCTA CCTCTCCAGT TCCGCTCTTG 
    TCCACTCCCC GACCTGCTGA TGTATCATCT GTCTCAGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951360.1
    CDS100..5667
    Translation

    Target ORF information:

    RefSeq Version XM_018095871.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis golgi brefeldin A resistant guanine nucleotide exchange factor 1 (gbf1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_018095871.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGTGGACA AGAATATCTA TATAGTCCAG GGAGAAATTA GCATTGTTGT TGGTGCAATA 
    AAACGCAATG CACGATGGAG CATGCACACT CCTGTGGATG AGGAACAGGA CCCTCTGCTA 
    CACAGCTTTA GTGTCCTAAA GGAAGTATTA AATAATATTA AAGAACTTTC TGAAATTGAG 
    CCCAATGTAT TCCTTCGTCC TTTCTTGGAA GTTATCCGTT CTGAAGACAC TACAGGCCCT 
    ATTACTGGAC TTGCTCTGAC GTCTGTCAAT AAGTTTCTTT CCTATGCACT TATAGATCCA 
    AGCCATGAAG GTACAGCTGA AGGAATTGAA AATATGGCAG ATGCAGTTAC ACATGCCCGA 
    TTTGTTGGCA CTGACCCAGC GAGTGACGAG GTTGTCTTGA TGAAAATCTT ACAGGTTTTG 
    CGGACTTTGC TTCTCACCCC AGTTGGAGCT CATTTAACAA ATGAATCCGT TTGTGAAATA 
    ATGCAATCTT GTTTTCGCAT ATGCTTTGAA ATGAGGCTGA GTGAGTTATT GAGAAAATCT 
    GCAGAGCACA CTCTTGTCGA CATGGTGCAG CTGCTCTTCT CAAGGTTACC ACAGTTTAAG 
    GAAGAAGCAA AGAGTTACAT TGGGACTAAC ATGAAAAAGT TGAAAATGAG AGCTGGAGGA 
    ATGAGTGACT CCTCAAAATG GAAAAAGCAG AAGAAATCTC CTAGGCAGCC ACGTCATGTT 
    ACCAAAGCTA TGGCGGCAGC AGAACCCTCC TCTCAGCAAC CAGTAGTTAA TGGCACCACA 
    ATTACTACTA ACCTCACTGG TGAAGTTCCT TTTATTGATG CTTCCTCTCC AGATGCTAAT 
    TTTCCCATTT CAGGGGTGAG TTCAGAGGCC ACGTCTGCTG TAGTCAGTCC TACCACAGAT 
    AGTGGTGTAG ATATGTCCTC TCAGGCAACA TCTAAAGAAG ACCTTTCTGA ACTGGATCCT 
    GCAAGTCCAT CTCTTTCTGC CATTCAGCAA TCAGGAGACA TTTGTCTTTC TGAACAGCAA 
    AACCAGGATG ATGATAAGAT TGAAGACCTT ATGGAAAGGC ATACATATTC TGCTTCTGTG 
    GAATCCATAC CAGAAGTATT AGATGATAAT GTGTCATTGG CTGATCAATC AGACTCCGCT 
    TCAGTCCATG ATATGGATTA TGTAAATCCT AGAGGTGTGA GATTTACCCA ATCTTCACAG 
    AAAGAAGGAA CAGTCTTAGT ACCATATGGA CTGCCATGCA TTCGGGAATT ATTTCGTTTT 
    CTTATTTCTT TAACCAACCC CCATGATCGA CATAACTCCG AAGTGATGAT ACACATGGGT 
    TTACAGTTGA TCACAGTTGC TCTTGAGTCT GCACCTATTG AAAATTATGT TTCGCTTTTA 
    GGCCTTGTAA AGGATGAACT TTGTCGACAC TTATTTCAGC TACTGAGCAT TGAGAGACTG 
    AACCTATATG CAGCTTCCTT GCGAGCATGT TTTCTTTTGT TTGAGGGTAT GAGGGAGCAT 
    TTGAAGTTCC AGCTAGAGAT GTACATAAAG AAGCTCATGG ATATTATTAC TGTGGAGAAC 
    CCTAAGATGC CTTATGAGAT GAAAGAAATG GCTCTTGAAG CTATTGTGCA ATTGTGGAGA 
    ATCCCGAGCT TTGTTACAGA ATTATACATT AATTACGACT GTGATTATTA TTGCTCCAAC 
    CTTTTTGAAG ATCTCACTAA ACTGCTTTCC AAGAATGCCT TTCCAGTTTC TGGACAGCTG 
    TACACCACTC ATCTCCTTTC CCTGGAAGCT CTGCTAACTG TCATTGATAG CACAGAATCA 
    CACTGCCAGG CTAAAAATCT AAGCAATGTG TCACAGGATA AGAAAGAAAC TGGAAAACCA 
    AGTGGAGATC TGTCAGAAGG GACCAAAGAT TCTGGAAATC TCAATGAACA CTCCATTGAA 
    GTTAAGCCAT TCACTGTTAG TGCAGAAAAC AATGGATCGA AGCCCCCCAC AAGTGGCCAC 
    TTAATGGCAG ATAAAATGAC TCTTGGGGTC CAAGAAACAG AGTACAGCAC TGATGGAGGT 
    GAGAAGAAGA CTTTAAAGAA GCCCCAACGC TTTTCCTGCT TACTTCCGAA TGTTCAAGAG 
    CTGAATGACA TTAAGACCAA AAAGAAGCTT CTTATAACTG GAACTGAGCA GTTCAACCAG 
    AAACCAAAGA AAGGAGTGCA GTTTCTTCAG GAAAAAGACC TTTTGGCAAC CCCCATGGAT 
    AATGCTGAGG TAGCACAGTG GCTCAGAGAA AATCCAAGGC TGGACAAGAA AATGATTGGG 
    GAGTTTGTGA GTGATCGCAA AAATCTGGAC TTACTTGAAA GTTTTGTAGG AACCTTCCAT 
    TTCCAAGGCC TGAGAGTTGA TGAAGCTTTA AGGCTTTACC TTGAAGCTTT TCGTTTGCCT 
    GGCGAGGCAC CTGTGATTCA GAGGTTACTG GAGGCTTTTA CAGAGCACTG GCGAAAATCC 
    AATGGAACTC CTTTTGCACA CAGTGATGCC TGCTTTGCTT TGGCTTATGC AGTTATCATG 
    CTGAACACAG ACCAACACAA TCACAATGTC CGCAAGCAAA ATGTGCCAAT GACTTTAGAG 
    GAATTCAGGA AAAATTTAAA GGGTGTTAAT GGTGGAAAAG ATTTTGACCA AGATATGCTA 
    GAGGATATTT ATCACGCAAT CAAAAATGAA GAAATTGTAA TGCCTGAAGA GCAGACTGGC 
    TTAGTAAAAG AGAATTATTT TTGGAATGTT TTGCTGCATC GGGGAGCCAC TCCAGAAGGA 
    ATGTTCTTGC ATGTGGATCC TGGAAGTTAT GACCATGACC TCTTTACTAT GACTTGGGGG 
    CCAACAATAG CTGCCTTGTC CTATGTGTTT GATAAGAGCA TGGATGAAAC GATTATTCAG 
    AAAGCAATAT CTGGTTTTAG GAAATGTGCA ATGATATCTG CTCACTATGG TCTCAGTGAT 
    GTTTTTGACA ATCTCATAAT TTCCTTGTGC AAGTTTACAA CACTTAGCAG TGAAGCCGTT 
    GAAAATCTTC CCACTGTTTT TGGAAGCAAC CTAAAAGCTC AGATCGCTGC AAAGACTGTG 
    TTCCACTTAT CCCACAGACA TGGTGACATC CTTCGAGAGG GATGGAAAAA TATTATGGAC 
    TCCATGCTGC AGCTTTTTAG AGCTGAGCTG CTTCCCAAGG CTATGGTTGA GGTTGAAGAT 
    TTTGTAGACC CCAATGGAAA AATATCTCTT CAACGTGAGG AAATCCCTGC CAACCGTGGG 
    GAATCCACTG TACTAAGTTT TGTCAGCTGG CTGACTCTGA GTGGGACAGA ACAGTCAAGT 
    CTAAGAGGAC CTTCTACTGA AAATCAAGAG GCCAAGAAAC TAGCGCTGGA ATGTATCAAG 
    TTATGTGATC CAGAGAAACT GATCACAGAG AGCAAGTTTC TTCAACTGGA GTCTTTGCAA 
    GAACTAATGA AGGCTCTGAT ATCTGTGACA CCAGATGAAG AAACGTATGA TGAAGAAGAT 
    GCTGCATTCT GCCTAGAAAT GTTAATGAGA ATTGTGCTAG AAAACAGAGA TCGTGTTGGC 
    TGTGTTTGGC AGGCTGTACG AGATCATCTC TATCGTCTAT GTGTCCATGC TGTAGAGTTC 
    TGCTTCCTGG TTGAAAGAGC AGTTGTAGGG CTGCTGCGCT TGGCAATACG CCTTTTACGC 
    AGAGAGGAAA TTAGTGGTCA GGTTATCCTC TCCTTGAGAA TTCTTTTAAT GATGAAACCA 
    ACTGTGTTGT CTAAAGTGAG TCGCCAAGTT GCTTTTGGTG TGCATGAGCT CTTGAAAACC 
    AATGCTGCTA ACATCCATTC CAGCAATGAC TGGTACACAC TATTCTGCCT TTTGGAGTGC 
    ATTGGGGCAG GAGTGAAGCC GCCCCCTGCC TTACAGGTGA CGACGAGAGC TGACAATGAC 
    ACAGGTGCGC AGTCTGACAG TGAAGTTTCC TCATACCATC CAAGTGAAGT GAATCTTGAT 
    CGTGGATACA CCTCAGACTC TGAGGTATAT GCAGAACACA GCAAGCAGTC AAAAATGCAC 
    CGCTCTGTGA CTGATGTGGA TATGATGAAC AGTGGCTGGC TGATGGTTGG AAAGGAAGAT 
    GTTGACTCTT CTAAAGTTGC TACAGTTATT AACAAACCTG TTGCTAATGC CCCAGTGAAT 
    CAATACAGCC TAACCATTGG TTTGGATTTT GGCCCTCACG ATACCAAATC TCTCATGAAG 
    AGCGTGGAGT CCCTTTCTTT TATTGTTCGA GATGCTGCAC ATGTCACACC AGAAAACTTT 
    GAACTCTGTG TTAAAACAAT CCGTGTCTTT GTAGAGGCAA GTCTAAATGG AGGGTACAAA 
    TCTCAGGAGA AAAGGGTAAA GAACCACAAA TATGACAGTA AATCCAACCG CTTCAAAAAG 
    AAAACCAAGG AAAAAGAAAA TTCTGTCCGC CGTACACGGG CTTCAAATCA GCGACAATAC 
    CGATCTCACA GTGATGATGA GGAAGATGAA AGTGTACCAG CCAGCTATCA CACTGTGTCA 
    TTACAGGTTA GTCATGATCT TTTGGACCTA ATGCACACAC TTCACACAAG AGCTGCCACC 
    ATATACAGTT CTTGGGCAGA GGAACAGCGC AACCTAGAGA CAGCTGCTGA GAAAATTACA 
    GCCGATTCTA AGACACTGTG GTCAAACTGT TGGTGTCCTT TACTGCAAGG TATTGCTTGG 
    CTCTGCTGTG ATGCTCGGCG CCAGGTCAGA ATGCAGGCTC TCACCTACCT GCAAAGGGCA 
    CTTTTAGTCC ATGACTTACA GGCTCTTGAT GCACTTGAAT GGGAATCATG CTTTAATAAG 
    GTGCTATTTC CATTGCTGAC CAAACTATTG GAAAATATTA GTCCAGCTGA TGTTGGCGGG 
    ATGGAGGAGA CCAGAATGAG AGGCTCCACT CTGCTCTCAA AGGTATTTCT ACAGCATCTG 
    TCTCCTCTTC TTTCTCTGTC GACATTTGCT GCCTTGTGGC TTACTATTCT TGATTTCATG 
    GACAAGTACA TGCATGCTGG CTCCAGTGAC TTACTGCCTG AGGCAATTCC AGAGTCACTG 
    AAAAATATGC TTCTCGTAAT GGACACTGCC GGAATATTCC ACAGTGCAGA CTCGCGGACG 
    GGCTACTCTG ATTTATGGGA GATCACGTGG GAAAGGATAG ATTGCTTTTT ACCTCGTCTA 
    AGGGAAGAAC TGTTTAAACA AACTGTGATA CAAGATCCAC CCAATATAGT TACAACAGAA 
    ACTCCTGTTG AGCCAAGACC TCCCCCTCAA TCAACTCAGT CAGAGAGACC AGCGGAGCAG 
    GGACCTTGTG ACTCAGAGAC TCAAAGTTTA GCAATCTCAT CTGCTCCAGT AGTTGGATCA 
    AGTTCTTCCT GCACCCCAGT TAGAGTCAGC CCTGTAACAG ATATACCTCC ACCTATAACT 
    CAGTCTCCAG TCATCCTGCA TCCTCTGACC TCCCCGCTGC AGGTGGGAGT GCCACCCATG 
    TCGCTGCCAA TCATCCTCAA CCCTGCATTA ATTGAAGCTA CCTCTCCAGT TCCGCTCTTG 
    TCCACTCCCC GACCTGCTGA TGTATCATCT GTCTCAGAGG TCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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