cntln cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cntln
Entrez Gene ID	100379901
Full Name	centlein
General protein information	Preferred Names centlein Names centlein centlein, centrosomal protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004910865.3	XP_004910922.1	centlein isoform X1
XM_004910865.4	XP_004910922.1	centlein isoform X1
XM_002935668.5	XP_002935714.2	centlein isoform X1
XM_004910866.3	XP_004910923.1	centlein isoform X2
XM_002935668.4	XP_002935714.2	centlein isoform X1
XM_004910866.4	XP_004910923.1	centlein isoform X2
XM_018097775.1	XP_017953264.1	centlein isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CNTLN	NP_060208.2
Pan troglodytes (chimpanzee)	CNTLN	XP_003312056.1
Rattus norvegicus (Norway rat)	Cntln	NP_001107874.1
Gallus gallus (chicken)	C9ORF39	XP_001232685.3
Danio rerio (zebrafish)	si:ch211-2i17.1	XP_005159969.1
Bos taurus (cattle)	CNTLN	NP_001179650.1
Macaca mulatta (Rhesus monkey)	CNTLN	XP_001102815.2
Canis lupus familiaris (dog)	CNTLN	XP_538669.3
Mus musculus (house mouse)	Cntln	NP_780484.2
Xenopus tropicalis (tropical clawed frog)	cntln	XP_004910922.1

Gene Ontology

The following cntln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cntln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04070	XM_004910865.4 Latest version!	Xenopus tropicalis centlein (cntln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa04070	XM_002935668.5 Latest version!	Xenopus tropicalis centlein (cntln), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa04071	XM_004910866.4 Latest version!	Xenopus tropicalis centlein (cntln), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa42089	XM_018097775.1 Latest version!	Xenopus tropicalis centlein (cntln), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OXa04070	XM_004910865.3	Xenopus tropicalis centlein (cntln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa04070	XM_002935668.4	Xenopus tropicalis centlein (cntln), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa04071	XM_004910866.3	Xenopus tropicalis centlein (cntln), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cntln ( XM_004910865.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910922.1 Xenopus tropicalis centlein (cntln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04070
Clone ID Related Accession (Same CDS sequence)	XM_004910865.3 , XM_004910865.4 , XM_002935668.5 , XM_002935668.4
Accession Version	XM_004910865.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centlein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGCAGAATT ATCTGCATGA GAGTGCAGCA 
AAGAAAAGAA ACATTACAAA ATGTTGCACC AGGCTCCATC CACCTGCTGT AAAATCAGGA 
CACCGAGAGA AGAGAAACAA AATCCTGGCA TACAGGCATA TTTCCTCTGT GTCATCGTTA 
AGGCAAAGAG TTGCATCCTT ACAGCACCAG GTGTCCGTGT TACAAAAAGG AAAGCAAGCA 
GCTAGCGCTT GTGCAGATGA GCTAAAGAAA GAGAAGATAA AACTAACAGC TGAACTGCAT 
CTGGCACAGC AAAGACTTCA AATCAGCAAG CAGATGGCAA AGAAATTGTC TGCAGAACTT 
ACTGAACTTC ATCGGGAAAG AGAGTATTTG GAAAGATCTG CAGAGCAGAT GAAGGAACAG 
CTGAATCTAG CCAAAAGATC ATCAGAAACG CAGCGCCTTT TGACCTCAGA TCAGTCTCCA 
GTCACACAAT TAACTGAATC AAAGAGTTCA GACCTCGAAA TAAAGCAGCT CCACACTAAA 
CTTAAGAATA TGACTGTTGA AATAACGAAA CAGACCACAA CGATTAAATA TTTGAAAAAT 
GAAGTCCAGG AAAAAGAGGA CCGTATTCGG GATCTGCAGG AAAGGGTTGC ACGAATGGAA 
AGGGATGTCA ACATGAAAAG GCATTTGATA GAGGATCAGA AAACTCGCCT GAAAACCTAC 
CAGGAAAACG AGAGACCTAG CAAAGAAATG TTAGAAAGTC TTGAAAGGAA GGTGAAGGCA 
TTGACAGAAG AATGTTCTCA CAAGAAGACA TCAATTGATT CATTAAAGCA GAGGCTTAGT 
GTAGCTACAA AAGAAAAGGC ACAGTATGAG CAGATGTACC ACAAAGTAAA AGAAGACATG 
GAAAAAAAGA CTGTTAAGGT CAGCAACCTT GAGACTAAAG TGACAGAATC TGAGCATGCC 
ATATCTAAAC TGGAAAACAC AGCCACTCAG CAACTTCACA ATTTAGCGGC GCAAAGTGAA 
CAAGCTTTAG AAGTCATTCA GAATAAACTG CTGTTGGTTA CCAGAAGGGC AGAGGAGCTT 
GCCATCTTTG TAAAGAGTCT AGCTAAAGAG CTGCTGTTAA ATATAGAAGA AATCAGAAAG 
GAAATCAGGC AGGCAAACTT ACTTCAAGAA GCAAACAATG GTCCATCCAA AAAATCTGTT 
CACAAGGCAC AATCTCTTGC AGCCTCTATC CTCAACATAT CATGCACTGA CCTGGATGAG 
ATATTAGATG TCCAGGATGA AGAGGAAGAA GCAAACATAA AGCGGTTGGT GGAATCTGAT 
AAAAAATGGG CGAACCACAT AATAAAGCTT CTGGAAGGAC AGCTTCCTTT TGCATCCAGC 
CTCATGAAAG CCGTGCAGGA AAAACTTAAT GAGAAGGCTA GACTTATGGA AGAATACACA 
CTGCTGAAGA GACATATCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910922.1
CDS	285..4208
Translation	MAAKKNYQECIRKLEEEVEHLNEELSQCQADKEFVWSLWKRLQVANPDITQAVGLVVDREKSKAESKDRKVLEILQAKDSKIQELEQRASGQQQEINNLVQRKIAVDEENTLLKKELNDLHEKLKDKSQELKSNKENTQEKEKQTAVILKNLEQEKEGLSKRCADLLNDLEKARKQEAQWKADKSGIDGKIKDMEVDLKEARKQMEAMHNKINGLSSQVIVKQTELAQRETDLTRLRKELQELQNLYKQSNEHASQQAELIQQLQALNLDTQKVLRNQEDAHTTETISYQKLYNDLNVCYDMAKSSEAQLRQSNIALTDQIHQKEQQISQLQVKLQEALSALPKSPQEPQDCEHPSLVELELIIASQKSEIKFLQDKLKTTDLQLTRVNNCGADALESSILRAGRKHEVQPAKRSRSLSPKGFSKESEELKKLKIAEKRIEHLEKALQIKMQENAEIRKAHERRRERLLTLQSNYREVKQQIEQSEEYARGKENKKRNQRADPRQLRHEDSDAVWNELAHFKRQNKKLHVEKMNLEEELDQIKVQASADKVAIQELNECLQQEREELLYILAKEEEIVSSTPKKNKDEVLQQALHKANRLEIRLCGLEAEVTKLKGINEELIAEKDSLKLLVTSLQIDADSKERKVQKLKTENIEAYEVREKLEMKNAELIYEITSLKILLEEGNKLQSENQDLIRQIENLQRALDQVRTVPVLATRELNSRQRSKKHTVNKVKQRAVKKISLRRHQAFLNQSIKVMSSVFENFSRDGWEDVIEDSDSESQTLGSEKEGIVKTAKSENLCMDYTNSADENTCSEYTHSGCEDASDELTYGKQNYLHESAAKKRNITKCCTRLHPPAVKSGHREKRNKILAYRHISSVSSLRQRVASLQHQVSVLQKGKQAASACADELKKEKIKLTAELHLAQQRLQISKQMAKKLSAELTELHREREYLERSAEQMKEQLNLAKRSSETQRLLTSDQSPVTQLTESKSSDLEIKQLHTKLKNMTVEITKQTTTIKYLKNEVQEKEDRIRDLQERVARMERDVNMKRHLIEDQKTRLKTYQENERPSKEMLESLERKVKALTEECSHKKTSIDSLKQRLSVATKEKAQYEQMYHKVKEDMEKKTVKVSNLETKVTESEHAISKLENTATQQLHNLAAQSEQALEVIQNKLLLVTRRAEELAIFVKSLAKELLLNIEEIRKEIRQANLLQEANNGPSKKSVHKAQSLAASILNISCTDLDEILDVQDEEEEANIKRLVESDKKWANHIIKLLEGQLPFASSLMKAVQEKLNEKARLMEEYTLLKRHIS

Target ORF information:

RefSeq Version	XM_004910865.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centlein (cntln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910865.3

ORF Insert Sequence:

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGCAGAATT ATCTGCATGA GAGTGCAGCA 
AAGAAAAGAA ACATTACAAA ATGTTGCACC AGGCTCCATC CACCTGCTGT AAAATCAGGA 
CACCGAGAGA AGAGAAACAA AATCCTGGCA TACAGGCATA TTTCCTCTGT GTCATCGTTA 
AGGCAAAGAG TTGCATCCTT ACAGCACCAG GTGTCCGTGT TACAAAAAGG AAAGCAAGCA 
GCTAGCGCTT GTGCAGATGA GCTAAAGAAA GAGAAGATAA AACTAACAGC TGAACTGCAT 
CTGGCACAGC AAAGACTTCA AATCAGCAAG CAGATGGCAA AGAAATTGTC TGCAGAACTT 
ACTGAACTTC ATCGGGAAAG AGAGTATTTG GAAAGATCTG CAGAGCAGAT GAAGGAACAG 
CTGAATCTAG CCAAAAGATC ATCAGAAACG CAGCGCCTTT TGACCTCAGA TCAGTCTCCA 
GTCACACAAT TAACTGAATC AAAGAGTTCA GACCTCGAAA TAAAGCAGCT CCACACTAAA 
CTTAAGAATA TGACTGTTGA AATAACGAAA CAGACCACAA CGATTAAATA TTTGAAAAAT 
GAAGTCCAGG AAAAAGAGGA CCGTATTCGG GATCTGCAGG AAAGGGTTGC ACGAATGGAA 
AGGGATGTCA ACATGAAAAG GCATTTGATA GAGGATCAGA AAACTCGCCT GAAAACCTAC 
CAGGAAAACG AGAGACCTAG CAAAGAAATG TTAGAAAGTC TTGAAAGGAA GGTGAAGGCA 
TTGACAGAAG AATGTTCTCA CAAGAAGACA TCAATTGATT CATTAAAGCA GAGGCTTAGT 
GTAGCTACAA AAGAAAAGGC ACAGTATGAG CAGATGTACC ACAAAGTAAA AGAAGACATG 
GAAAAAAAGA CTGTTAAGGT CAGCAACCTT GAGACTAAAG TGACAGAATC TGAGCATGCC 
ATATCTAAAC TGGAAAACAC AGCCACTCAG CAACTTCACA ATTTAGCGGC GCAAAGTGAA 
CAAGCTTTAG AAGTCATTCA GAATAAACTG CTGTTGGTTA CCAGAAGGGC AGAGGAGCTT 
GCCATCTTTG TAAAGAGTCT AGCTAAAGAG CTGCTGTTAA ATATAGAAGA AATCAGAAAG 
GAAATCAGGC AGGCAAACTT ACTTCAAGAA GCAAACAATG GTCCATCCAA AAAATCTGTT 
CACAAGGCAC AATCTCTTGC AGCCTCTATC CTCAACATAT CATGCACTGA CCTGGATGAG 
ATATTAGATG TCCAGGATGA AGAGGAAGAA GCAAACATAA AGCGGTTGGT GGAATCTGAT 
AAAAAATGGG CGAACCACAT AATAAAGCTT CTGGAAGGAC AGCTTCCTTT TGCATCCAGC 
CTCATGAAAG CCGTGCAGGA AAAACTTAAT GAGAAGGCTA GACTTATGGA AGAATACACA 
CTGCTGAAGA GACATATCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cntln ( XM_004910865.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910922.1 Xenopus tropicalis centlein (cntln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04070
Clone ID Related Accession (Same CDS sequence)	XM_004910865.3 , XM_004910865.4 , XM_002935668.5 , XM_002935668.4
Accession Version	XM_004910865.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centlein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGCAGAATT ATCTGCATGA GAGTGCAGCA 
AAGAAAAGAA ACATTACAAA ATGTTGCACC AGGCTCCATC CACCTGCTGT AAAATCAGGA 
CACCGAGAGA AGAGAAACAA AATCCTGGCA TACAGGCATA TTTCCTCTGT GTCATCGTTA 
AGGCAAAGAG TTGCATCCTT ACAGCACCAG GTGTCCGTGT TACAAAAAGG AAAGCAAGCA 
GCTAGCGCTT GTGCAGATGA GCTAAAGAAA GAGAAGATAA AACTAACAGC TGAACTGCAT 
CTGGCACAGC AAAGACTTCA AATCAGCAAG CAGATGGCAA AGAAATTGTC TGCAGAACTT 
ACTGAACTTC ATCGGGAAAG AGAGTATTTG GAAAGATCTG CAGAGCAGAT GAAGGAACAG 
CTGAATCTAG CCAAAAGATC ATCAGAAACG CAGCGCCTTT TGACCTCAGA TCAGTCTCCA 
GTCACACAAT TAACTGAATC AAAGAGTTCA GACCTCGAAA TAAAGCAGCT CCACACTAAA 
CTTAAGAATA TGACTGTTGA AATAACGAAA CAGACCACAA CGATTAAATA TTTGAAAAAT 
GAAGTCCAGG AAAAAGAGGA CCGTATTCGG GATCTGCAGG AAAGGGTTGC ACGAATGGAA 
AGGGATGTCA ACATGAAAAG GCATTTGATA GAGGATCAGA AAACTCGCCT GAAAACCTAC 
CAGGAAAACG AGAGACCTAG CAAAGAAATG TTAGAAAGTC TTGAAAGGAA GGTGAAGGCA 
TTGACAGAAG AATGTTCTCA CAAGAAGACA TCAATTGATT CATTAAAGCA GAGGCTTAGT 
GTAGCTACAA AAGAAAAGGC ACAGTATGAG CAGATGTACC ACAAAGTAAA AGAAGACATG 
GAAAAAAAGA CTGTTAAGGT CAGCAACCTT GAGACTAAAG TGACAGAATC TGAGCATGCC 
ATATCTAAAC TGGAAAACAC AGCCACTCAG CAACTTCACA ATTTAGCGGC GCAAAGTGAA 
CAAGCTTTAG AAGTCATTCA GAATAAACTG CTGTTGGTTA CCAGAAGGGC AGAGGAGCTT 
GCCATCTTTG TAAAGAGTCT AGCTAAAGAG CTGCTGTTAA ATATAGAAGA AATCAGAAAG 
GAAATCAGGC AGGCAAACTT ACTTCAAGAA GCAAACAATG GTCCATCCAA AAAATCTGTT 
CACAAGGCAC AATCTCTTGC AGCCTCTATC CTCAACATAT CATGCACTGA CCTGGATGAG 
ATATTAGATG TCCAGGATGA AGAGGAAGAA GCAAACATAA AGCGGTTGGT GGAATCTGAT 
AAAAAATGGG CGAACCACAT AATAAAGCTT CTGGAAGGAC AGCTTCCTTT TGCATCCAGC 
CTCATGAAAG CCGTGCAGGA AAAACTTAAT GAGAAGGCTA GACTTATGGA AGAATACACA 
CTGCTGAAGA GACATATCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910922.1
CDS	275..4198
Translation	MAAKKNYQECIRKLEEEVEHLNEELSQCQADKEFVWSLWKRLQVANPDITQAVGLVVDREKSKAESKDRKVLEILQAKDSKIQELEQRASGQQQEINNLVQRKIAVDEENTLLKKELNDLHEKLKDKSQELKSNKENTQEKEKQTAVILKNLEQEKEGLSKRCADLLNDLEKARKQEAQWKADKSGIDGKIKDMEVDLKEARKQMEAMHNKINGLSSQVIVKQTELAQRETDLTRLRKELQELQNLYKQSNEHASQQAELIQQLQALNLDTQKVLRNQEDAHTTETISYQKLYNDLNVCYDMAKSSEAQLRQSNIALTDQIHQKEQQISQLQVKLQEALSALPKSPQEPQDCEHPSLVELELIIASQKSEIKFLQDKLKTTDLQLTRVNNCGADALESSILRAGRKHEVQPAKRSRSLSPKGFSKESEELKKLKIAEKRIEHLEKALQIKMQENAEIRKAHERRRERLLTLQSNYREVKQQIEQSEEYARGKENKKRNQRADPRQLRHEDSDAVWNELAHFKRQNKKLHVEKMNLEEELDQIKVQASADKVAIQELNECLQQEREELLYILAKEEEIVSSTPKKNKDEVLQQALHKANRLEIRLCGLEAEVTKLKGINEELIAEKDSLKLLVTSLQIDADSKERKVQKLKTENIEAYEVREKLEMKNAELIYEITSLKILLEEGNKLQSENQDLIRQIENLQRALDQVRTVPVLATRELNSRQRSKKHTVNKVKQRAVKKISLRRHQAFLNQSIKVMSSVFENFSRDGWEDVIEDSDSESQTLGSEKEGIVKTAKSENLCMDYTNSADENTCSEYTHSGCEDASDELTYGKQNYLHESAAKKRNITKCCTRLHPPAVKSGHREKRNKILAYRHISSVSSLRQRVASLQHQVSVLQKGKQAASACADELKKEKIKLTAELHLAQQRLQISKQMAKKLSAELTELHREREYLERSAEQMKEQLNLAKRSSETQRLLTSDQSPVTQLTESKSSDLEIKQLHTKLKNMTVEITKQTTTIKYLKNEVQEKEDRIRDLQERVARMERDVNMKRHLIEDQKTRLKTYQENERPSKEMLESLERKVKALTEECSHKKTSIDSLKQRLSVATKEKAQYEQMYHKVKEDMEKKTVKVSNLETKVTESEHAISKLENTATQQLHNLAAQSEQALEVIQNKLLLVTRRAEELAIFVKSLAKELLLNIEEIRKEIRQANLLQEANNGPSKKSVHKAQSLAASILNISCTDLDEILDVQDEEEEANIKRLVESDKKWANHIIKLLEGQLPFASSLMKAVQEKLNEKARLMEEYTLLKRHIS

Target ORF information:

RefSeq Version	XM_004910865.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centlein (cntln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910865.4

ORF Insert Sequence:

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGCAGAATT ATCTGCATGA GAGTGCAGCA 
AAGAAAAGAA ACATTACAAA ATGTTGCACC AGGCTCCATC CACCTGCTGT AAAATCAGGA 
CACCGAGAGA AGAGAAACAA AATCCTGGCA TACAGGCATA TTTCCTCTGT GTCATCGTTA 
AGGCAAAGAG TTGCATCCTT ACAGCACCAG GTGTCCGTGT TACAAAAAGG AAAGCAAGCA 
GCTAGCGCTT GTGCAGATGA GCTAAAGAAA GAGAAGATAA AACTAACAGC TGAACTGCAT 
CTGGCACAGC AAAGACTTCA AATCAGCAAG CAGATGGCAA AGAAATTGTC TGCAGAACTT 
ACTGAACTTC ATCGGGAAAG AGAGTATTTG GAAAGATCTG CAGAGCAGAT GAAGGAACAG 
CTGAATCTAG CCAAAAGATC ATCAGAAACG CAGCGCCTTT TGACCTCAGA TCAGTCTCCA 
GTCACACAAT TAACTGAATC AAAGAGTTCA GACCTCGAAA TAAAGCAGCT CCACACTAAA 
CTTAAGAATA TGACTGTTGA AATAACGAAA CAGACCACAA CGATTAAATA TTTGAAAAAT 
GAAGTCCAGG AAAAAGAGGA CCGTATTCGG GATCTGCAGG AAAGGGTTGC ACGAATGGAA 
AGGGATGTCA ACATGAAAAG GCATTTGATA GAGGATCAGA AAACTCGCCT GAAAACCTAC 
CAGGAAAACG AGAGACCTAG CAAAGAAATG TTAGAAAGTC TTGAAAGGAA GGTGAAGGCA 
TTGACAGAAG AATGTTCTCA CAAGAAGACA TCAATTGATT CATTAAAGCA GAGGCTTAGT 
GTAGCTACAA AAGAAAAGGC ACAGTATGAG CAGATGTACC ACAAAGTAAA AGAAGACATG 
GAAAAAAAGA CTGTTAAGGT CAGCAACCTT GAGACTAAAG TGACAGAATC TGAGCATGCC 
ATATCTAAAC TGGAAAACAC AGCCACTCAG CAACTTCACA ATTTAGCGGC GCAAAGTGAA 
CAAGCTTTAG AAGTCATTCA GAATAAACTG CTGTTGGTTA CCAGAAGGGC AGAGGAGCTT 
GCCATCTTTG TAAAGAGTCT AGCTAAAGAG CTGCTGTTAA ATATAGAAGA AATCAGAAAG 
GAAATCAGGC AGGCAAACTT ACTTCAAGAA GCAAACAATG GTCCATCCAA AAAATCTGTT 
CACAAGGCAC AATCTCTTGC AGCCTCTATC CTCAACATAT CATGCACTGA CCTGGATGAG 
ATATTAGATG TCCAGGATGA AGAGGAAGAA GCAAACATAA AGCGGTTGGT GGAATCTGAT 
AAAAAATGGG CGAACCACAT AATAAAGCTT CTGGAAGGAC AGCTTCCTTT TGCATCCAGC 
CTCATGAAAG CCGTGCAGGA AAAACTTAAT GAGAAGGCTA GACTTATGGA AGAATACACA 
CTGCTGAAGA GACATATCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cntln ( XM_002935668.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935714.2 Xenopus tropicalis centlein (cntln), transcript variant X2, mRNA.

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CloneID	OXa04070
Clone ID Related Accession (Same CDS sequence)	XM_004910865.3 , XM_004910865.4 , XM_002935668.5 , XM_002935668.4
Accession Version	XM_002935668.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centlein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935668.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGCAGAATT ATCTGCATGA GAGTGCAGCA 
AAGAAAAGAA ACATTACAAA ATGTTGCACC AGGCTCCATC CACCTGCTGT AAAATCAGGA 
CACCGAGAGA AGAGAAACAA AATCCTGGCA TACAGGCATA TTTCCTCTGT GTCATCGTTA 
AGGCAAAGAG TTGCATCCTT ACAGCACCAG GTGTCCGTGT TACAAAAAGG AAAGCAAGCA 
GCTAGCGCTT GTGCAGATGA GCTAAAGAAA GAGAAGATAA AACTAACAGC TGAACTGCAT 
CTGGCACAGC AAAGACTTCA AATCAGCAAG CAGATGGCAA AGAAATTGTC TGCAGAACTT 
ACTGAACTTC ATCGGGAAAG AGAGTATTTG GAAAGATCTG CAGAGCAGAT GAAGGAACAG 
CTGAATCTAG CCAAAAGATC ATCAGAAACG CAGCGCCTTT TGACCTCAGA TCAGTCTCCA 
GTCACACAAT TAACTGAATC AAAGAGTTCA GACCTCGAAA TAAAGCAGCT CCACACTAAA 
CTTAAGAATA TGACTGTTGA AATAACGAAA CAGACCACAA CGATTAAATA TTTGAAAAAT 
GAAGTCCAGG AAAAAGAGGA CCGTATTCGG GATCTGCAGG AAAGGGTTGC ACGAATGGAA 
AGGGATGTCA ACATGAAAAG GCATTTGATA GAGGATCAGA AAACTCGCCT GAAAACCTAC 
CAGGAAAACG AGAGACCTAG CAAAGAAATG TTAGAAAGTC TTGAAAGGAA GGTGAAGGCA 
TTGACAGAAG AATGTTCTCA CAAGAAGACA TCAATTGATT CATTAAAGCA GAGGCTTAGT 
GTAGCTACAA AAGAAAAGGC ACAGTATGAG CAGATGTACC ACAAAGTAAA AGAAGACATG 
GAAAAAAAGA CTGTTAAGGT CAGCAACCTT GAGACTAAAG TGACAGAATC TGAGCATGCC 
ATATCTAAAC TGGAAAACAC AGCCACTCAG CAACTTCACA ATTTAGCGGC GCAAAGTGAA 
CAAGCTTTAG AAGTCATTCA GAATAAACTG CTGTTGGTTA CCAGAAGGGC AGAGGAGCTT 
GCCATCTTTG TAAAGAGTCT AGCTAAAGAG CTGCTGTTAA ATATAGAAGA AATCAGAAAG 
GAAATCAGGC AGGCAAACTT ACTTCAAGAA GCAAACAATG GTCCATCCAA AAAATCTGTT 
CACAAGGCAC AATCTCTTGC AGCCTCTATC CTCAACATAT CATGCACTGA CCTGGATGAG 
ATATTAGATG TCCAGGATGA AGAGGAAGAA GCAAACATAA AGCGGTTGGT GGAATCTGAT 
AAAAAATGGG CGAACCACAT AATAAAGCTT CTGGAAGGAC AGCTTCCTTT TGCATCCAGC 
CTCATGAAAG CCGTGCAGGA AAAACTTAAT GAGAAGGCTA GACTTATGGA AGAATACACA 
CTGCTGAAGA GACATATCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935714.2
CDS	190..4113
Translation	MAAKKNYQECIRKLEEEVEHLNEELSQCQADKEFVWSLWKRLQVANPDITQAVGLVVDREKSKAESKDRKVLEILQAKDSKIQELEQRASGQQQEINNLVQRKIAVDEENTLLKKELNDLHEKLKDKSQELKSNKENTQEKEKQTAVILKNLEQEKEGLSKRCADLLNDLEKARKQEAQWKADKSGIDGKIKDMEVDLKEARKQMEAMHNKINGLSSQVIVKQTELAQRETDLTRLRKELQELQNLYKQSNEHASQQAELIQQLQALNLDTQKVLRNQEDAHTTETISYQKLYNDLNVCYDMAKSSEAQLRQSNIALTDQIHQKEQQISQLQVKLQEALSALPKSPQEPQDCEHPSLVELELIIASQKSEIKFLQDKLKTTDLQLTRVNNCGADALESSILRAGRKHEVQPAKRSRSLSPKGFSKESEELKKLKIAEKRIEHLEKALQIKMQENAEIRKAHERRRERLLTLQSNYREVKQQIEQSEEYARGKENKKRNQRADPRQLRHEDSDAVWNELAHFKRQNKKLHVEKMNLEEELDQIKVQASADKVAIQELNECLQQEREELLYILAKEEEIVSSTPKKNKDEVLQQALHKANRLEIRLCGLEAEVTKLKGINEELIAEKDSLKLLVTSLQIDADSKERKVQKLKTENIEAYEVREKLEMKNAELIYEITSLKILLEEGNKLQSENQDLIRQIENLQRALDQVRTVPVLATRELNSRQRSKKHTVNKVKQRAVKKISLRRHQAFLNQSIKVMSSVFENFSRDGWEDVIEDSDSESQTLGSEKEGIVKTAKSENLCMDYTNSADENTCSEYTHSGCEDASDELTYGKQNYLHESAAKKRNITKCCTRLHPPAVKSGHREKRNKILAYRHISSVSSLRQRVASLQHQVSVLQKGKQAASACADELKKEKIKLTAELHLAQQRLQISKQMAKKLSAELTELHREREYLERSAEQMKEQLNLAKRSSETQRLLTSDQSPVTQLTESKSSDLEIKQLHTKLKNMTVEITKQTTTIKYLKNEVQEKEDRIRDLQERVARMERDVNMKRHLIEDQKTRLKTYQENERPSKEMLESLERKVKALTEECSHKKTSIDSLKQRLSVATKEKAQYEQMYHKVKEDMEKKTVKVSNLETKVTESEHAISKLENTATQQLHNLAAQSEQALEVIQNKLLLVTRRAEELAIFVKSLAKELLLNIEEIRKEIRQANLLQEANNGPSKKSVHKAQSLAASILNISCTDLDEILDVQDEEEEANIKRLVESDKKWANHIIKLLEGQLPFASSLMKAVQEKLNEKARLMEEYTLLKRHIS

Target ORF information:

RefSeq Version	XM_002935668.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centlein (cntln), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935668.5

ORF Insert Sequence:

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGCAGAATT ATCTGCATGA GAGTGCAGCA 
AAGAAAAGAA ACATTACAAA ATGTTGCACC AGGCTCCATC CACCTGCTGT AAAATCAGGA 
CACCGAGAGA AGAGAAACAA AATCCTGGCA TACAGGCATA TTTCCTCTGT GTCATCGTTA 
AGGCAAAGAG TTGCATCCTT ACAGCACCAG GTGTCCGTGT TACAAAAAGG AAAGCAAGCA 
GCTAGCGCTT GTGCAGATGA GCTAAAGAAA GAGAAGATAA AACTAACAGC TGAACTGCAT 
CTGGCACAGC AAAGACTTCA AATCAGCAAG CAGATGGCAA AGAAATTGTC TGCAGAACTT 
ACTGAACTTC ATCGGGAAAG AGAGTATTTG GAAAGATCTG CAGAGCAGAT GAAGGAACAG 
CTGAATCTAG CCAAAAGATC ATCAGAAACG CAGCGCCTTT TGACCTCAGA TCAGTCTCCA 
GTCACACAAT TAACTGAATC AAAGAGTTCA GACCTCGAAA TAAAGCAGCT CCACACTAAA 
CTTAAGAATA TGACTGTTGA AATAACGAAA CAGACCACAA CGATTAAATA TTTGAAAAAT 
GAAGTCCAGG AAAAAGAGGA CCGTATTCGG GATCTGCAGG AAAGGGTTGC ACGAATGGAA 
AGGGATGTCA ACATGAAAAG GCATTTGATA GAGGATCAGA AAACTCGCCT GAAAACCTAC 
CAGGAAAACG AGAGACCTAG CAAAGAAATG TTAGAAAGTC TTGAAAGGAA GGTGAAGGCA 
TTGACAGAAG AATGTTCTCA CAAGAAGACA TCAATTGATT CATTAAAGCA GAGGCTTAGT 
GTAGCTACAA AAGAAAAGGC ACAGTATGAG CAGATGTACC ACAAAGTAAA AGAAGACATG 
GAAAAAAAGA CTGTTAAGGT CAGCAACCTT GAGACTAAAG TGACAGAATC TGAGCATGCC 
ATATCTAAAC TGGAAAACAC AGCCACTCAG CAACTTCACA ATTTAGCGGC GCAAAGTGAA 
CAAGCTTTAG AAGTCATTCA GAATAAACTG CTGTTGGTTA CCAGAAGGGC AGAGGAGCTT 
GCCATCTTTG TAAAGAGTCT AGCTAAAGAG CTGCTGTTAA ATATAGAAGA AATCAGAAAG 
GAAATCAGGC AGGCAAACTT ACTTCAAGAA GCAAACAATG GTCCATCCAA AAAATCTGTT 
CACAAGGCAC AATCTCTTGC AGCCTCTATC CTCAACATAT CATGCACTGA CCTGGATGAG 
ATATTAGATG TCCAGGATGA AGAGGAAGAA GCAAACATAA AGCGGTTGGT GGAATCTGAT 
AAAAAATGGG CGAACCACAT AATAAAGCTT CTGGAAGGAC AGCTTCCTTT TGCATCCAGC 
CTCATGAAAG CCGTGCAGGA AAAACTTAAT GAGAAGGCTA GACTTATGGA AGAATACACA 
CTGCTGAAGA GACATATCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cntln ( XM_002935668.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935714.2 Xenopus tropicalis centlein (cntln), transcript variant X2, mRNA.

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CloneID	OXa04070
Clone ID Related Accession (Same CDS sequence)	XM_004910865.3 , XM_004910865.4 , XM_002935668.5 , XM_002935668.4
Accession Version	XM_002935668.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centlein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935668.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGCAGAATT ATCTGCATGA GAGTGCAGCA 
AAGAAAAGAA ACATTACAAA ATGTTGCACC AGGCTCCATC CACCTGCTGT AAAATCAGGA 
CACCGAGAGA AGAGAAACAA AATCCTGGCA TACAGGCATA TTTCCTCTGT GTCATCGTTA 
AGGCAAAGAG TTGCATCCTT ACAGCACCAG GTGTCCGTGT TACAAAAAGG AAAGCAAGCA 
GCTAGCGCTT GTGCAGATGA GCTAAAGAAA GAGAAGATAA AACTAACAGC TGAACTGCAT 
CTGGCACAGC AAAGACTTCA AATCAGCAAG CAGATGGCAA AGAAATTGTC TGCAGAACTT 
ACTGAACTTC ATCGGGAAAG AGAGTATTTG GAAAGATCTG CAGAGCAGAT GAAGGAACAG 
CTGAATCTAG CCAAAAGATC ATCAGAAACG CAGCGCCTTT TGACCTCAGA TCAGTCTCCA 
GTCACACAAT TAACTGAATC AAAGAGTTCA GACCTCGAAA TAAAGCAGCT CCACACTAAA 
CTTAAGAATA TGACTGTTGA AATAACGAAA CAGACCACAA CGATTAAATA TTTGAAAAAT 
GAAGTCCAGG AAAAAGAGGA CCGTATTCGG GATCTGCAGG AAAGGGTTGC ACGAATGGAA 
AGGGATGTCA ACATGAAAAG GCATTTGATA GAGGATCAGA AAACTCGCCT GAAAACCTAC 
CAGGAAAACG AGAGACCTAG CAAAGAAATG TTAGAAAGTC TTGAAAGGAA GGTGAAGGCA 
TTGACAGAAG AATGTTCTCA CAAGAAGACA TCAATTGATT CATTAAAGCA GAGGCTTAGT 
GTAGCTACAA AAGAAAAGGC ACAGTATGAG CAGATGTACC ACAAAGTAAA AGAAGACATG 
GAAAAAAAGA CTGTTAAGGT CAGCAACCTT GAGACTAAAG TGACAGAATC TGAGCATGCC 
ATATCTAAAC TGGAAAACAC AGCCACTCAG CAACTTCACA ATTTAGCGGC GCAAAGTGAA 
CAAGCTTTAG AAGTCATTCA GAATAAACTG CTGTTGGTTA CCAGAAGGGC AGAGGAGCTT 
GCCATCTTTG TAAAGAGTCT AGCTAAAGAG CTGCTGTTAA ATATAGAAGA AATCAGAAAG 
GAAATCAGGC AGGCAAACTT ACTTCAAGAA GCAAACAATG GTCCATCCAA AAAATCTGTT 
CACAAGGCAC AATCTCTTGC AGCCTCTATC CTCAACATAT CATGCACTGA CCTGGATGAG 
ATATTAGATG TCCAGGATGA AGAGGAAGAA GCAAACATAA AGCGGTTGGT GGAATCTGAT 
AAAAAATGGG CGAACCACAT AATAAAGCTT CTGGAAGGAC AGCTTCCTTT TGCATCCAGC 
CTCATGAAAG CCGTGCAGGA AAAACTTAAT GAGAAGGCTA GACTTATGGA AGAATACACA 
CTGCTGAAGA GACATATCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935714.2
CDS	200..4123
Translation	MAAKKNYQECIRKLEEEVEHLNEELSQCQADKEFVWSLWKRLQVANPDITQAVGLVVDREKSKAESKDRKVLEILQAKDSKIQELEQRASGQQQEINNLVQRKIAVDEENTLLKKELNDLHEKLKDKSQELKSNKENTQEKEKQTAVILKNLEQEKEGLSKRCADLLNDLEKARKQEAQWKADKSGIDGKIKDMEVDLKEARKQMEAMHNKINGLSSQVIVKQTELAQRETDLTRLRKELQELQNLYKQSNEHASQQAELIQQLQALNLDTQKVLRNQEDAHTTETISYQKLYNDLNVCYDMAKSSEAQLRQSNIALTDQIHQKEQQISQLQVKLQEALSALPKSPQEPQDCEHPSLVELELIIASQKSEIKFLQDKLKTTDLQLTRVNNCGADALESSILRAGRKHEVQPAKRSRSLSPKGFSKESEELKKLKIAEKRIEHLEKALQIKMQENAEIRKAHERRRERLLTLQSNYREVKQQIEQSEEYARGKENKKRNQRADPRQLRHEDSDAVWNELAHFKRQNKKLHVEKMNLEEELDQIKVQASADKVAIQELNECLQQEREELLYILAKEEEIVSSTPKKNKDEVLQQALHKANRLEIRLCGLEAEVTKLKGINEELIAEKDSLKLLVTSLQIDADSKERKVQKLKTENIEAYEVREKLEMKNAELIYEITSLKILLEEGNKLQSENQDLIRQIENLQRALDQVRTVPVLATRELNSRQRSKKHTVNKVKQRAVKKISLRRHQAFLNQSIKVMSSVFENFSRDGWEDVIEDSDSESQTLGSEKEGIVKTAKSENLCMDYTNSADENTCSEYTHSGCEDASDELTYGKQNYLHESAAKKRNITKCCTRLHPPAVKSGHREKRNKILAYRHISSVSSLRQRVASLQHQVSVLQKGKQAASACADELKKEKIKLTAELHLAQQRLQISKQMAKKLSAELTELHREREYLERSAEQMKEQLNLAKRSSETQRLLTSDQSPVTQLTESKSSDLEIKQLHTKLKNMTVEITKQTTTIKYLKNEVQEKEDRIRDLQERVARMERDVNMKRHLIEDQKTRLKTYQENERPSKEMLESLERKVKALTEECSHKKTSIDSLKQRLSVATKEKAQYEQMYHKVKEDMEKKTVKVSNLETKVTESEHAISKLENTATQQLHNLAAQSEQALEVIQNKLLLVTRRAEELAIFVKSLAKELLLNIEEIRKEIRQANLLQEANNGPSKKSVHKAQSLAASILNISCTDLDEILDVQDEEEEANIKRLVESDKKWANHIIKLLEGQLPFASSLMKAVQEKLNEKARLMEEYTLLKRHIS

Target ORF information:

RefSeq Version	XM_002935668.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centlein (cntln), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935668.4

ORF Insert Sequence:

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGCAGAATT ATCTGCATGA GAGTGCAGCA 
AAGAAAAGAA ACATTACAAA ATGTTGCACC AGGCTCCATC CACCTGCTGT AAAATCAGGA 
CACCGAGAGA AGAGAAACAA AATCCTGGCA TACAGGCATA TTTCCTCTGT GTCATCGTTA 
AGGCAAAGAG TTGCATCCTT ACAGCACCAG GTGTCCGTGT TACAAAAAGG AAAGCAAGCA 
GCTAGCGCTT GTGCAGATGA GCTAAAGAAA GAGAAGATAA AACTAACAGC TGAACTGCAT 
CTGGCACAGC AAAGACTTCA AATCAGCAAG CAGATGGCAA AGAAATTGTC TGCAGAACTT 
ACTGAACTTC ATCGGGAAAG AGAGTATTTG GAAAGATCTG CAGAGCAGAT GAAGGAACAG 
CTGAATCTAG CCAAAAGATC ATCAGAAACG CAGCGCCTTT TGACCTCAGA TCAGTCTCCA 
GTCACACAAT TAACTGAATC AAAGAGTTCA GACCTCGAAA TAAAGCAGCT CCACACTAAA 
CTTAAGAATA TGACTGTTGA AATAACGAAA CAGACCACAA CGATTAAATA TTTGAAAAAT 
GAAGTCCAGG AAAAAGAGGA CCGTATTCGG GATCTGCAGG AAAGGGTTGC ACGAATGGAA 
AGGGATGTCA ACATGAAAAG GCATTTGATA GAGGATCAGA AAACTCGCCT GAAAACCTAC 
CAGGAAAACG AGAGACCTAG CAAAGAAATG TTAGAAAGTC TTGAAAGGAA GGTGAAGGCA 
TTGACAGAAG AATGTTCTCA CAAGAAGACA TCAATTGATT CATTAAAGCA GAGGCTTAGT 
GTAGCTACAA AAGAAAAGGC ACAGTATGAG CAGATGTACC ACAAAGTAAA AGAAGACATG 
GAAAAAAAGA CTGTTAAGGT CAGCAACCTT GAGACTAAAG TGACAGAATC TGAGCATGCC 
ATATCTAAAC TGGAAAACAC AGCCACTCAG CAACTTCACA ATTTAGCGGC GCAAAGTGAA 
CAAGCTTTAG AAGTCATTCA GAATAAACTG CTGTTGGTTA CCAGAAGGGC AGAGGAGCTT 
GCCATCTTTG TAAAGAGTCT AGCTAAAGAG CTGCTGTTAA ATATAGAAGA AATCAGAAAG 
GAAATCAGGC AGGCAAACTT ACTTCAAGAA GCAAACAATG GTCCATCCAA AAAATCTGTT 
CACAAGGCAC AATCTCTTGC AGCCTCTATC CTCAACATAT CATGCACTGA CCTGGATGAG 
ATATTAGATG TCCAGGATGA AGAGGAAGAA GCAAACATAA AGCGGTTGGT GGAATCTGAT 
AAAAAATGGG CGAACCACAT AATAAAGCTT CTGGAAGGAC AGCTTCCTTT TGCATCCAGC 
CTCATGAAAG CCGTGCAGGA AAAACTTAAT GAGAAGGCTA GACTTATGGA AGAATACACA 
CTGCTGAAGA GACATATCTC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cntln ( XM_004910866.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910923.1 Xenopus tropicalis centlein (cntln), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa04071
Clone ID Related Accession (Same CDS sequence)	XM_004910866.3 , XM_004910866.4
Accession Version	XM_004910866.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centlein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGAATTATC TGCATGAGAG TGCAGCAAAG 
AAAAGAAACA TTACAAAATG TTGCACCAGG CTCCATCCAC CTGCTGTAAA ATCAGGACAC 
CGAGAGAAGA GAAACAAAAT CCTGGCATAC AGGCATATTT CCTCTGTGTC ATCGTTAAGG 
CAAAGAGTTG CATCCTTACA GCACCAGGTG TCCGTGTTAC AAAAAGGAAA GCAAGCAGCT 
AGCGCTTGTG CAGATGAGCT AAAGAAAGAG AAGATAAAAC TAACAGCTGA ACTGCATCTG 
GCACAGCAAA GACTTCAAAT CAGCAAGCAG ATGGCAAAGA AATTGTCTGC AGAACTTACT 
GAACTTCATC GGGAAAGAGA GTATTTGGAA AGATCTGCAG AGCAGATGAA GGAACAGCTG 
AATCTAGCCA AAAGATCATC AGAAACGCAG CGCCTTTTGA CCTCAGATCA GTCTCCAGTC 
ACACAATTAA CTGAATCAAA GAGTTCAGAC CTCGAAATAA AGCAGCTCCA CACTAAACTT 
AAGAATATGA CTGTTGAAAT AACGAAACAG ACCACAACGA TTAAATATTT GAAAAATGAA 
GTCCAGGAAA AAGAGGACCG TATTCGGGAT CTGCAGGAAA GGGTTGCACG AATGGAAAGG 
GATGTCAACA TGAAAAGGCA TTTGATAGAG GATCAGAAAA CTCGCCTGAA AACCTACCAG 
GAAAACGAGA GACCTAGCAA AGAAATGTTA GAAAGTCTTG AAAGGAAGGT GAAGGCATTG 
ACAGAAGAAT GTTCTCACAA GAAGACATCA ATTGATTCAT TAAAGCAGAG GCTTAGTGTA 
GCTACAAAAG AAAAGGCACA GTATGAGCAG ATGTACCACA AAGTAAAAGA AGACATGGAA 
AAAAAGACTG TTAAGGTCAG CAACCTTGAG ACTAAAGTGA CAGAATCTGA GCATGCCATA 
TCTAAACTGG AAAACACAGC CACTCAGCAA CTTCACAATT TAGCGGCGCA AAGTGAACAA 
GCTTTAGAAG TCATTCAGAA TAAACTGCTG TTGGTTACCA GAAGGGCAGA GGAGCTTGCC 
ATCTTTGTAA AGAGTCTAGC TAAAGAGCTG CTGTTAAATA TAGAAGAAAT CAGAAAGGAA 
ATCAGGCAGG CAAACTTACT TCAAGAAGCA AACAATGGTC CATCCAAAAA ATCTGTTCAC 
AAGGCACAAT CTCTTGCAGC CTCTATCCTC AACATATCAT GCACTGACCT GGATGAGATA 
TTAGATGTCC AGGATGAAGA GGAAGAAGCA AACATAAAGC GGTTGGTGGA ATCTGATAAA 
AAATGGGCGA ACCACATAAT AAAGCTTCTG GAAGGACAGC TTCCTTTTGC ATCCAGCCTC 
ATGAAAGCCG TGCAGGAAAA ACTTAATGAG AAGGCTAGAC TTATGGAAGA ATACACACTG 
CTGAAGAGAC ATATCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910923.1
CDS	285..4205
Translation	MAAKKNYQECIRKLEEEVEHLNEELSQCQADKEFVWSLWKRLQVANPDITQAVGLVVDREKSKAESKDRKVLEILQAKDSKIQELEQRASGQQQEINNLVQRKIAVDEENTLLKKELNDLHEKLKDKSQELKSNKENTQEKEKQTAVILKNLEQEKEGLSKRCADLLNDLEKARKQEAQWKADKSGIDGKIKDMEVDLKEARKQMEAMHNKINGLSSQVIVKQTELAQRETDLTRLRKELQELQNLYKQSNEHASQQAELIQQLQALNLDTQKVLRNQEDAHTTETISYQKLYNDLNVCYDMAKSSEAQLRQSNIALTDQIHQKEQQISQLQVKLQEALSALPKSPQEPQDCEHPSLVELELIIASQKSEIKFLQDKLKTTDLQLTRVNNCGADALESSILRAGRKHEVQPAKRSRSLSPKGFSKESEELKKLKIAEKRIEHLEKALQIKMQENAEIRKAHERRRERLLTLQSNYREVKQQIEQSEEYARGKENKKRNQRADPRQLRHEDSDAVWNELAHFKRQNKKLHVEKMNLEEELDQIKVQASADKVAIQELNECLQQEREELLYILAKEEEIVSSTPKKNKDEVLQQALHKANRLEIRLCGLEAEVTKLKGINEELIAEKDSLKLLVTSLQIDADSKERKVQKLKTENIEAYEVREKLEMKNAELIYEITSLKILLEEGNKLQSENQDLIRQIENLQRALDQVRTVPVLATRELNSRQRSKKHTVNKVKQRAVKKISLRRHQAFLNQSIKVMSSVFENFSRDGWEDVIEDSDSESQTLGSEKEGIVKTAKSENLCMDYTNSADENTCSEYTHSGCEDASDELTYGKNYLHESAAKKRNITKCCTRLHPPAVKSGHREKRNKILAYRHISSVSSLRQRVASLQHQVSVLQKGKQAASACADELKKEKIKLTAELHLAQQRLQISKQMAKKLSAELTELHREREYLERSAEQMKEQLNLAKRSSETQRLLTSDQSPVTQLTESKSSDLEIKQLHTKLKNMTVEITKQTTTIKYLKNEVQEKEDRIRDLQERVARMERDVNMKRHLIEDQKTRLKTYQENERPSKEMLESLERKVKALTEECSHKKTSIDSLKQRLSVATKEKAQYEQMYHKVKEDMEKKTVKVSNLETKVTESEHAISKLENTATQQLHNLAAQSEQALEVIQNKLLLVTRRAEELAIFVKSLAKELLLNIEEIRKEIRQANLLQEANNGPSKKSVHKAQSLAASILNISCTDLDEILDVQDEEEEANIKRLVESDKKWANHIIKLLEGQLPFASSLMKAVQEKLNEKARLMEEYTLLKRHIS

Target ORF information:

RefSeq Version	XM_004910866.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centlein (cntln), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910866.3

ORF Insert Sequence:

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGAATTATC TGCATGAGAG TGCAGCAAAG 
AAAAGAAACA TTACAAAATG TTGCACCAGG CTCCATCCAC CTGCTGTAAA ATCAGGACAC 
CGAGAGAAGA GAAACAAAAT CCTGGCATAC AGGCATATTT CCTCTGTGTC ATCGTTAAGG 
CAAAGAGTTG CATCCTTACA GCACCAGGTG TCCGTGTTAC AAAAAGGAAA GCAAGCAGCT 
AGCGCTTGTG CAGATGAGCT AAAGAAAGAG AAGATAAAAC TAACAGCTGA ACTGCATCTG 
GCACAGCAAA GACTTCAAAT CAGCAAGCAG ATGGCAAAGA AATTGTCTGC AGAACTTACT 
GAACTTCATC GGGAAAGAGA GTATTTGGAA AGATCTGCAG AGCAGATGAA GGAACAGCTG 
AATCTAGCCA AAAGATCATC AGAAACGCAG CGCCTTTTGA CCTCAGATCA GTCTCCAGTC 
ACACAATTAA CTGAATCAAA GAGTTCAGAC CTCGAAATAA AGCAGCTCCA CACTAAACTT 
AAGAATATGA CTGTTGAAAT AACGAAACAG ACCACAACGA TTAAATATTT GAAAAATGAA 
GTCCAGGAAA AAGAGGACCG TATTCGGGAT CTGCAGGAAA GGGTTGCACG AATGGAAAGG 
GATGTCAACA TGAAAAGGCA TTTGATAGAG GATCAGAAAA CTCGCCTGAA AACCTACCAG 
GAAAACGAGA GACCTAGCAA AGAAATGTTA GAAAGTCTTG AAAGGAAGGT GAAGGCATTG 
ACAGAAGAAT GTTCTCACAA GAAGACATCA ATTGATTCAT TAAAGCAGAG GCTTAGTGTA 
GCTACAAAAG AAAAGGCACA GTATGAGCAG ATGTACCACA AAGTAAAAGA AGACATGGAA 
AAAAAGACTG TTAAGGTCAG CAACCTTGAG ACTAAAGTGA CAGAATCTGA GCATGCCATA 
TCTAAACTGG AAAACACAGC CACTCAGCAA CTTCACAATT TAGCGGCGCA AAGTGAACAA 
GCTTTAGAAG TCATTCAGAA TAAACTGCTG TTGGTTACCA GAAGGGCAGA GGAGCTTGCC 
ATCTTTGTAA AGAGTCTAGC TAAAGAGCTG CTGTTAAATA TAGAAGAAAT CAGAAAGGAA 
ATCAGGCAGG CAAACTTACT TCAAGAAGCA AACAATGGTC CATCCAAAAA ATCTGTTCAC 
AAGGCACAAT CTCTTGCAGC CTCTATCCTC AACATATCAT GCACTGACCT GGATGAGATA 
TTAGATGTCC AGGATGAAGA GGAAGAAGCA AACATAAAGC GGTTGGTGGA ATCTGATAAA 
AAATGGGCGA ACCACATAAT AAAGCTTCTG GAAGGACAGC TTCCTTTTGC ATCCAGCCTC 
ATGAAAGCCG TGCAGGAAAA ACTTAATGAG AAGGCTAGAC TTATGGAAGA ATACACACTG 
CTGAAGAGAC ATATCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cntln ( XM_004910866.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910923.1 Xenopus tropicalis centlein (cntln), transcript variant X3, mRNA.

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CloneID	OXa04071
Clone ID Related Accession (Same CDS sequence)	XM_004910866.3 , XM_004910866.4
Accession Version	XM_004910866.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centlein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910866.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGAATTATC TGCATGAGAG TGCAGCAAAG 
AAAAGAAACA TTACAAAATG TTGCACCAGG CTCCATCCAC CTGCTGTAAA ATCAGGACAC 
CGAGAGAAGA GAAACAAAAT CCTGGCATAC AGGCATATTT CCTCTGTGTC ATCGTTAAGG 
CAAAGAGTTG CATCCTTACA GCACCAGGTG TCCGTGTTAC AAAAAGGAAA GCAAGCAGCT 
AGCGCTTGTG CAGATGAGCT AAAGAAAGAG AAGATAAAAC TAACAGCTGA ACTGCATCTG 
GCACAGCAAA GACTTCAAAT CAGCAAGCAG ATGGCAAAGA AATTGTCTGC AGAACTTACT 
GAACTTCATC GGGAAAGAGA GTATTTGGAA AGATCTGCAG AGCAGATGAA GGAACAGCTG 
AATCTAGCCA AAAGATCATC AGAAACGCAG CGCCTTTTGA CCTCAGATCA GTCTCCAGTC 
ACACAATTAA CTGAATCAAA GAGTTCAGAC CTCGAAATAA AGCAGCTCCA CACTAAACTT 
AAGAATATGA CTGTTGAAAT AACGAAACAG ACCACAACGA TTAAATATTT GAAAAATGAA 
GTCCAGGAAA AAGAGGACCG TATTCGGGAT CTGCAGGAAA GGGTTGCACG AATGGAAAGG 
GATGTCAACA TGAAAAGGCA TTTGATAGAG GATCAGAAAA CTCGCCTGAA AACCTACCAG 
GAAAACGAGA GACCTAGCAA AGAAATGTTA GAAAGTCTTG AAAGGAAGGT GAAGGCATTG 
ACAGAAGAAT GTTCTCACAA GAAGACATCA ATTGATTCAT TAAAGCAGAG GCTTAGTGTA 
GCTACAAAAG AAAAGGCACA GTATGAGCAG ATGTACCACA AAGTAAAAGA AGACATGGAA 
AAAAAGACTG TTAAGGTCAG CAACCTTGAG ACTAAAGTGA CAGAATCTGA GCATGCCATA 
TCTAAACTGG AAAACACAGC CACTCAGCAA CTTCACAATT TAGCGGCGCA AAGTGAACAA 
GCTTTAGAAG TCATTCAGAA TAAACTGCTG TTGGTTACCA GAAGGGCAGA GGAGCTTGCC 
ATCTTTGTAA AGAGTCTAGC TAAAGAGCTG CTGTTAAATA TAGAAGAAAT CAGAAAGGAA 
ATCAGGCAGG CAAACTTACT TCAAGAAGCA AACAATGGTC CATCCAAAAA ATCTGTTCAC 
AAGGCACAAT CTCTTGCAGC CTCTATCCTC AACATATCAT GCACTGACCT GGATGAGATA 
TTAGATGTCC AGGATGAAGA GGAAGAAGCA AACATAAAGC GGTTGGTGGA ATCTGATAAA 
AAATGGGCGA ACCACATAAT AAAGCTTCTG GAAGGACAGC TTCCTTTTGC ATCCAGCCTC 
ATGAAAGCCG TGCAGGAAAA ACTTAATGAG AAGGCTAGAC TTATGGAAGA ATACACACTG 
CTGAAGAGAC ATATCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910923.1
CDS	275..4195
Translation	MAAKKNYQECIRKLEEEVEHLNEELSQCQADKEFVWSLWKRLQVANPDITQAVGLVVDREKSKAESKDRKVLEILQAKDSKIQELEQRASGQQQEINNLVQRKIAVDEENTLLKKELNDLHEKLKDKSQELKSNKENTQEKEKQTAVILKNLEQEKEGLSKRCADLLNDLEKARKQEAQWKADKSGIDGKIKDMEVDLKEARKQMEAMHNKINGLSSQVIVKQTELAQRETDLTRLRKELQELQNLYKQSNEHASQQAELIQQLQALNLDTQKVLRNQEDAHTTETISYQKLYNDLNVCYDMAKSSEAQLRQSNIALTDQIHQKEQQISQLQVKLQEALSALPKSPQEPQDCEHPSLVELELIIASQKSEIKFLQDKLKTTDLQLTRVNNCGADALESSILRAGRKHEVQPAKRSRSLSPKGFSKESEELKKLKIAEKRIEHLEKALQIKMQENAEIRKAHERRRERLLTLQSNYREVKQQIEQSEEYARGKENKKRNQRADPRQLRHEDSDAVWNELAHFKRQNKKLHVEKMNLEEELDQIKVQASADKVAIQELNECLQQEREELLYILAKEEEIVSSTPKKNKDEVLQQALHKANRLEIRLCGLEAEVTKLKGINEELIAEKDSLKLLVTSLQIDADSKERKVQKLKTENIEAYEVREKLEMKNAELIYEITSLKILLEEGNKLQSENQDLIRQIENLQRALDQVRTVPVLATRELNSRQRSKKHTVNKVKQRAVKKISLRRHQAFLNQSIKVMSSVFENFSRDGWEDVIEDSDSESQTLGSEKEGIVKTAKSENLCMDYTNSADENTCSEYTHSGCEDASDELTYGKNYLHESAAKKRNITKCCTRLHPPAVKSGHREKRNKILAYRHISSVSSLRQRVASLQHQVSVLQKGKQAASACADELKKEKIKLTAELHLAQQRLQISKQMAKKLSAELTELHREREYLERSAEQMKEQLNLAKRSSETQRLLTSDQSPVTQLTESKSSDLEIKQLHTKLKNMTVEITKQTTTIKYLKNEVQEKEDRIRDLQERVARMERDVNMKRHLIEDQKTRLKTYQENERPSKEMLESLERKVKALTEECSHKKTSIDSLKQRLSVATKEKAQYEQMYHKVKEDMEKKTVKVSNLETKVTESEHAISKLENTATQQLHNLAAQSEQALEVIQNKLLLVTRRAEELAIFVKSLAKELLLNIEEIRKEIRQANLLQEANNGPSKKSVHKAQSLAASILNISCTDLDEILDVQDEEEEANIKRLVESDKKWANHIIKLLEGQLPFASSLMKAVQEKLNEKARLMEEYTLLKRHIS

Target ORF information:

RefSeq Version	XM_004910866.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centlein (cntln), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910866.4

ORF Insert Sequence:

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGAGCATCA GGGCAACAGC AAGAAATAAA CAACTTAGTT 
CAGAGAAAAA TAGCTGTAGA TGAAGAAAAT ACACTCCTGA AGAAGGAACT GAATGACCTC 
CACGAAAAAT TAAAAGATAA AAGTCAAGAA CTTAAGAGTA ATAAAGAGAA TACGCAAGAA 
AAGGAAAAAC AGACAGCAGT GATTTTAAAA AATCTAGAGC AGGAAAAGGA GGGGTTGAGT 
AAGAGATGTG CTGACCTGCT CAATGACCTG GAGAAAGCAA GGAAGCAGGA GGCACAGTGG 
AAAGCAGACA AATCTGGCAT TGATGGCAAA ATAAAGGATA TGGAAGTTGA CTTGAAAGAA 
GCAAGAAAAC AGATGGAGGC TATGCACAAC AAAATAAATG GGCTGTCATC ACAGGTCATT 
GTAAAGCAGA CAGAGTTGGC ACAGAGAGAA ACTGATTTGA CCAGGCTGAG GAAGGAGTTG 
CAAGAACTGC AAAATCTATA CAAGCAAAGC AATGAACATG CATCACAGCA AGCTGAGCTG 
ATCCAACAGC TTCAGGCTCT CAATTTGGAT ACTCAAAAGG TACTAAGAAA TCAGGAAGAT 
GCTCACACCA CTGAAACAAT CTCATACCAG AAGCTTTATA ATGACTTGAA TGTCTGTTAT 
GATATGGCAA AGTCCAGTGA GGCTCAACTC CGGCAGAGTA ATATTGCTCT CACAGACCAG 
ATACATCAAA AGGAACAGCA GATTAGCCAG CTTCAAGTGA AACTGCAAGA AGCCCTCAGT 
GCATTACCCA AGTCACCTCA AGAACCTCAG GACTGTGAAC ATCCATCCTT GGTGGAACTG 
GAACTCATAA TAGCTTCTCA GAAATCAGAA ATCAAATTTC TACAGGACAA GCTTAAAACA 
ACAGACCTTC AGTTAACTCG AGTAAATAAT TGTGGTGCCG ATGCACTTGA GAGCAGCATC 
CTGAGGGCTG GGAGAAAGCA CGAGGTGCAA CCTGCGAAAC GTTCAAGGTC TCTGTCCCCA 
AAAGGATTTT CCAAAGAGTC AGAAGAGTTA AAGAAGCTTA AGATAGCAGA AAAGAGAATT 
GAGCATTTAG AGAAAGCACT CCAGATTAAG ATGCAGGAAA ATGCTGAAAT CCGAAAAGCT 
CATGAAAGAC GAAGAGAGAG GCTACTGACA TTGCAAAGTA ATTATAGGGA AGTTAAACAA 
CAGATAGAGC AATCTGAAGA GTATGCCAGG GGAAAAGAAA ATAAAAAGCG AAACCAGCGT 
GCAGATCCAA GGCAACTTCG CCATGAAGAC TCTGATGCAG TGTGGAATGA GCTTGCTCAC 
TTTAAGAGGC AAAACAAAAA GCTTCATGTT GAAAAAATGA ACTTAGAAGA GGAACTAGAT 
CAAATCAAGG TGCAGGCATC TGCAGACAAA GTAGCTATAC AAGAATTAAA TGAGTGTCTT 
CAGCAAGAAC GAGAAGAACT GCTTTATATA CTTGCTAAAG AGGAAGAGAT CGTCAGCAGC 
ACCCCGAAGA AAAATAAAGA TGAGGTTTTG CAACAGGCCT TGCACAAGGC AAATCGTTTG 
GAAATACGTC TATGTGGGTT AGAAGCAGAA GTTACAAAAT TAAAAGGAAT AAATGAAGAA 
TTAATTGCAG AGAAAGATTC TTTAAAATTA TTGGTAACTA GTCTGCAAAT AGATGCAGAC 
TCTAAGGAAA GAAAAGTACA AAAACTAAAG ACTGAAAATA TAGAAGCATA TGAAGTAAGA 
GAGAAGTTAG AGATGAAGAA TGCAGAACTA ATTTATGAGA TCACATCACT CAAGATACTA 
TTGGAAGAAG GTAACAAGCT GCAAAGTGAA AACCAGGATC TCATAAGGCA AATAGAGAAC 
CTACAACGTG CTCTGGACCA GGTTAGAACA GTCCCTGTTC TGGCCACTAG AGAGTTGAAC 
AGTAGACAAA GAAGTAAAAA GCATACAGTA AACAAGGTTA AACAAAGAGC AGTCAAGAAG 
ATCTCTTTAA GACGTCACCA AGCTTTTCTT AATCAGTCTA TCAAGGTTAT GAGCAGTGTG 
TTTGAGAACT TCAGCAGAGA CGGATGGGAA GATGTTATTG AAGACAGTGA TTCAGAGTCC 
CAAACTTTGG GGAGTGAGAA AGAAGGAATT GTTAAAACAG CCAAGTCAGA AAATTTATGT 
ATGGATTACA CAAACTCAGC TGATGAAAAC ACTTGTTCTG AGTATACTCA TTCAGGGTGT 
GAAGATGCAA GTGATGAACT GACCTATGGC AAGAATTATC TGCATGAGAG TGCAGCAAAG 
AAAAGAAACA TTACAAAATG TTGCACCAGG CTCCATCCAC CTGCTGTAAA ATCAGGACAC 
CGAGAGAAGA GAAACAAAAT CCTGGCATAC AGGCATATTT CCTCTGTGTC ATCGTTAAGG 
CAAAGAGTTG CATCCTTACA GCACCAGGTG TCCGTGTTAC AAAAAGGAAA GCAAGCAGCT 
AGCGCTTGTG CAGATGAGCT AAAGAAAGAG AAGATAAAAC TAACAGCTGA ACTGCATCTG 
GCACAGCAAA GACTTCAAAT CAGCAAGCAG ATGGCAAAGA AATTGTCTGC AGAACTTACT 
GAACTTCATC GGGAAAGAGA GTATTTGGAA AGATCTGCAG AGCAGATGAA GGAACAGCTG 
AATCTAGCCA AAAGATCATC AGAAACGCAG CGCCTTTTGA CCTCAGATCA GTCTCCAGTC 
ACACAATTAA CTGAATCAAA GAGTTCAGAC CTCGAAATAA AGCAGCTCCA CACTAAACTT 
AAGAATATGA CTGTTGAAAT AACGAAACAG ACCACAACGA TTAAATATTT GAAAAATGAA 
GTCCAGGAAA AAGAGGACCG TATTCGGGAT CTGCAGGAAA GGGTTGCACG AATGGAAAGG 
GATGTCAACA TGAAAAGGCA TTTGATAGAG GATCAGAAAA CTCGCCTGAA AACCTACCAG 
GAAAACGAGA GACCTAGCAA AGAAATGTTA GAAAGTCTTG AAAGGAAGGT GAAGGCATTG 
ACAGAAGAAT GTTCTCACAA GAAGACATCA ATTGATTCAT TAAAGCAGAG GCTTAGTGTA 
GCTACAAAAG AAAAGGCACA GTATGAGCAG ATGTACCACA AAGTAAAAGA AGACATGGAA 
AAAAAGACTG TTAAGGTCAG CAACCTTGAG ACTAAAGTGA CAGAATCTGA GCATGCCATA 
TCTAAACTGG AAAACACAGC CACTCAGCAA CTTCACAATT TAGCGGCGCA AAGTGAACAA 
GCTTTAGAAG TCATTCAGAA TAAACTGCTG TTGGTTACCA GAAGGGCAGA GGAGCTTGCC 
ATCTTTGTAA AGAGTCTAGC TAAAGAGCTG CTGTTAAATA TAGAAGAAAT CAGAAAGGAA 
ATCAGGCAGG CAAACTTACT TCAAGAAGCA AACAATGGTC CATCCAAAAA ATCTGTTCAC 
AAGGCACAAT CTCTTGCAGC CTCTATCCTC AACATATCAT GCACTGACCT GGATGAGATA 
TTAGATGTCC AGGATGAAGA GGAAGAAGCA AACATAAAGC GGTTGGTGGA ATCTGATAAA 
AAATGGGCGA ACCACATAAT AAAGCTTCTG GAAGGACAGC TTCCTTTTGC ATCCAGCCTC 
ATGAAAGCCG TGCAGGAAAA ACTTAATGAG AAGGCTAGAC TTATGGAAGA ATACACACTG 
CTGAAGAGAC ATATCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cntln ( XM_018097775.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017953264.1 Xenopus tropicalis centlein (cntln), transcript variant X4, mRNA.

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CloneID	OXa42089
Clone ID Related Accession (Same CDS sequence)	XM_018097775.1
Accession Version	XM_018097775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	centlein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGTAATAAA GAGAATACGC AAGAAAAGGA AAAACAGACA 
GCAGTGATTT TAAAAAATCT AGAGCAGGAA AAGGAGGGGT TGAGTAAGAG ATGTGCTGAC 
CTGCTCAATG ACCTGGAGAA AGCAAGGAAG CAGGAGGCAC AGTGGAAAGC AGACAAATCT 
GGCATTGATG GCAAAATAAA GGATATGGAA GTTGACTTGA AAGAAGCAAG AAAACAGATG 
GAGGCTATGC ACAACAAAAT AAATGGGCTG TCATCACAGG TCATTGTAAA GCAGACAGAG 
TTGGCACAGA GAGAAACTGA TTTGACCAGG CTGAGGAAGG AGTTGCAAGA ACTGCAAAAT 
CTATACAAGC AAAGCAATGA ACATGCATCA CAGCAAGCTG AGCTGATCCA ACAGCTTCAG 
GCTCTCAATT TGGATACTCA AAAGGTACTA AGAAATCAGG AAGATGCTCA CACCACTGAA 
ACAATCTCAT ACCAGAAGCT TTATAATGAC TTGAATGTCT GTTATGATAT GGCAAAGTCC 
AGTGAGGCTC AACTCCGGCA GAGTAATATT GCTCTCACAG ACCAGATACA TCAAAAGGAA 
CAGCAGATTA GCCAGCTTCA AGTGAAACTG CAAGAAGCCC TCAGTGCATT ACCCAAGTCA 
CCTCAAGAAC CTCAGGACTG TGAACATCCA TCCTTGGTGG AACTGGAACT CATAATAGCT 
TCTCAGAAAT CAGAAATCAA ATTTCTACAG GACAAGCTTA AAACAACAGA CCTTCAGTTA 
ACTCGAGTAA ATAATTGTGG TGCCGATGCA CTTGAGAGCA GCATCCTGAG GGCTGGGAGA 
AAGCACGAGG TGCAACCTGC GAAACGTTCA AGGTCTCTGT CCCCAAAAGG ATTTTCCAAA 
GAGTCAGAAG AGTTAAAGAA GCTTAAGATA GCAGAAAAGA GAATTGAGCA TTTAGAGAAA 
GCACTCCAGA TTAAGATGCA GGAAAATGCT GAAATCCGAA AAGCTCATGA AAGACGAAGA 
GAGAGGCTAC TGACATTGCA AAGTAATTAT AGGGAAGTTA AACAACAGAT AGAGCAATCT 
GAAGAGTATG CCAGGGGAAA AGAAAATAAA AAGCGAAACC AGCGTGCAGA TCCAAGGCAA 
CTTCGCCATG AAGACTCTGA TGCAGTGTGG AATGAGCTTG CTCACTTTAA GAGGCAAAAC 
AAAAAGCTTC ATGTTGAAAA AATGAACTTA GAAGAGGAAC TAGATCAAAT CAAGGTGCAG 
GCATCTGCAG ACAAAGTAGC TATACAAGAA TTAAATGAGT GTCTTCAGCA AGAACGAGAA 
GAACTGCTTT ATATACTTGC TAAAGAGGAA GAGATCGTCA GCAGCACCCC GAAGAAAAAT 
AAAGATGAGG TTTTGCAACA GGCCTTGCAC AAGGCAAATC GTTTGGAAAT ACGTCTATGT 
GGGTTAGAAG CAGAAGTTAC AAAATTAAAA GGAATAAATG AAGAATTAAT TGCAGAGAAA 
GATTCTTTAA AATTATTGGT AACTAGTCTG CAAATAGATG CAGACTCTAA GGAAAGAAAA 
GTACAAAAAC TAAAGACTGA AAATATAGAA GCATATGAAG TAAGAGAGAA GTTAGAGATG 
AAGAATGCAG AACTAATTTA TGAGATCACA TCACTCAAGA TACTATTGGA AGAAGGTAAC 
AAGCTGCAAA GTGAAAACCA GGATCTCATA AGGCAAATAG AGAACCTACA ACGTGCTCTG 
GACCAGGTTA GAACAGTCCC TGTTCTGGCC ACTAGAGAGT TGAACAGTAG ACAAAGAAGT 
AAAAAGCATA CAGTAAACAA GGTTAAACAA AGAGCAGTCA AGAAGATCTC TTTAAGACGT 
CACCAAGCTT TTCTTAATCA GTCTATCAAG GTTATGAGCA GTGTGTTTGA GAACTTCAGC 
AGAGACGGAT GGGAAGATGT TATTGAAGAC AGTGATTCAG AGTCCCAAAC TTTGGGGAGT 
GAGAAAGAAG GAATTGTTAA AACAGCCAAG TCAGAAAATT TATGTATGGA TTACACAAAC 
TCAGCTGATG AAAACACTTG TTCTGAGTAT ACTCATTCAG GGTGTGAAGA TGCAAGTGAT 
GAACTGACCT ATGGCAAGCA GAATTATCTG CATGAGAGTG CAGCAAAGAA AAGAAACATT 
ACAAAATGTT GCACCAGGCT CCATCCACCT GCTGTAAAAT CAGGACACCG AGAGAAGAGA 
AACAAAATCC TGGCATACAG GCATATTTCC TCTGTGTCAT CGTTAAGGCA AAGAGTTGCA 
TCCTTACAGC ACCAGGTGTC CGTGTTACAA AAAGGAAAGC AAGCAGCTAG CGCTTGTGCA 
GATGAGCTAA AGAAAGAGAA GATAAAACTA ACAGCTGAAC TGCATCTGGC ACAGCAAAGA 
CTTCAAATCA GCAAGCAGAT GGCAAAGAAA TTGTCTGCAG AACTTACTGA ACTTCATCGG 
GAAAGAGAGT ATTTGGAAAG ATCTGCAGAG CAGATGAAGG AACAGCTGAA TCTAGCCAAA 
AGATCATCAG AAACGCAGCG CCTTTTGACC TCAGATCAGT CTCCAGTCAC ACAATTAACT 
GAATCAAAGA GTTCAGACCT CGAAATAAAG CAGCTCCACA CTAAACTTAA GAATATGACT 
GTTGAAATAA CGAAACAGAC CACAACGATT AAATATTTGA AAAATGAAGT CCAGGAAAAA 
GAGGACCGTA TTCGGGATCT GCAGGAAAGG GTTGCACGAA TGGAAAGGGA TGTCAACATG 
AAAAGGCATT TGATAGAGGA TCAGAAAACT CGCCTGAAAA CCTACCAGGA AAACGAGAGA 
CCTAGCAAAG AAATGTTAGA AAGTCTTGAA AGGAAGGTGA AGGCATTGAC AGAAGAATGT 
TCTCACAAGA AGACATCAAT TGATTCATTA AAGCAGAGGC TTAGTGTAGC TACAAAAGAA 
AAGGCACAGT ATGAGCAGAT GTACCACAAA GTAAAAGAAG ACATGGAAAA AAAGACTGTT 
AAGGTCAGCA ACCTTGAGAC TAAAGTGACA GAATCTGAGC ATGCCATATC TAAACTGGAA 
AACACAGCCA CTCAGCAACT TCACAATTTA GCGGCGCAAA GTGAACAAGC TTTAGAAGTC 
ATTCAGAATA AACTGCTGTT GGTTACCAGA AGGGCAGAGG AGCTTGCCAT CTTTGTAAAG 
AGTCTAGCTA AAGAGCTGCT GTTAAATATA GAAGAAATCA GAAAGGAAAT CAGGCAGGCA 
AACTTACTTC AAGAAGCAAA CAATGGTCCA TCCAAAAAAT CTGTTCACAA GGCACAATCT 
CTTGCAGCCT CTATCCTCAA CATATCATGC ACTGACCTGG ATGAGATATT AGATGTCCAG 
GATGAAGAGG AAGAAGCAAA CATAAAGCGG TTGGTGGAAT CTGATAAAAA ATGGGCGAAC 
CACATAATAA AGCTTCTGGA AGGACAGCTT CCTTTTGCAT CCAGCCTCAT GAAAGCCGTG 
CAGGAAAAAC TTAATGAGAA GGCTAGACTT ATGGAAGAAT ACACACTGCT GAAGAGACAT 
ATCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017953264.1
CDS	285..4073
Translation	MAAKKNYQECIRKLEEEVEHLNEELSQCQADKEFVWSLWKRLQVANPDITQAVGLVVDREKSKAESKDRKVLEILQAKDSKIQELEQSNKENTQEKEKQTAVILKNLEQEKEGLSKRCADLLNDLEKARKQEAQWKADKSGIDGKIKDMEVDLKEARKQMEAMHNKINGLSSQVIVKQTELAQRETDLTRLRKELQELQNLYKQSNEHASQQAELIQQLQALNLDTQKVLRNQEDAHTTETISYQKLYNDLNVCYDMAKSSEAQLRQSNIALTDQIHQKEQQISQLQVKLQEALSALPKSPQEPQDCEHPSLVELELIIASQKSEIKFLQDKLKTTDLQLTRVNNCGADALESSILRAGRKHEVQPAKRSRSLSPKGFSKESEELKKLKIAEKRIEHLEKALQIKMQENAEIRKAHERRRERLLTLQSNYREVKQQIEQSEEYARGKENKKRNQRADPRQLRHEDSDAVWNELAHFKRQNKKLHVEKMNLEEELDQIKVQASADKVAIQELNECLQQEREELLYILAKEEEIVSSTPKKNKDEVLQQALHKANRLEIRLCGLEAEVTKLKGINEELIAEKDSLKLLVTSLQIDADSKERKVQKLKTENIEAYEVREKLEMKNAELIYEITSLKILLEEGNKLQSENQDLIRQIENLQRALDQVRTVPVLATRELNSRQRSKKHTVNKVKQRAVKKISLRRHQAFLNQSIKVMSSVFENFSRDGWEDVIEDSDSESQTLGSEKEGIVKTAKSENLCMDYTNSADENTCSEYTHSGCEDASDELTYGKQNYLHESAAKKRNITKCCTRLHPPAVKSGHREKRNKILAYRHISSVSSLRQRVASLQHQVSVLQKGKQAASACADELKKEKIKLTAELHLAQQRLQISKQMAKKLSAELTELHREREYLERSAEQMKEQLNLAKRSSETQRLLTSDQSPVTQLTESKSSDLEIKQLHTKLKNMTVEITKQTTTIKYLKNEVQEKEDRIRDLQERVARMERDVNMKRHLIEDQKTRLKTYQENERPSKEMLESLERKVKALTEECSHKKTSIDSLKQRLSVATKEKAQYEQMYHKVKEDMEKKTVKVSNLETKVTESEHAISKLENTATQQLHNLAAQSEQALEVIQNKLLLVTRRAEELAIFVKSLAKELLLNIEEIRKEIRQANLLQEANNGPSKKSVHKAQSLAASILNISCTDLDEILDVQDEEEEANIKRLVESDKKWANHIIKLLEGQLPFASSLMKAVQEKLNEKARLMEEYTLLKRHIS

Target ORF information:

RefSeq Version	XM_018097775.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis centlein (cntln), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097775.1

ORF Insert Sequence:

ATGGCAGCAA AGAAAAATTA TCAAGAATGC ATTCGTAAAC TTGAAGAAGA AGTAGAGCAC 
TTAAATGAAG AGTTGTCTCA GTGCCAGGCA GACAAAGAAT TTGTATGGTC ATTGTGGAAG 
CGTCTACAGG TGGCTAATCC CGATATCACA CAAGCAGTTG GTTTAGTTGT AGACAGAGAA 
AAGAGTAAAG CAGAGTCCAA AGACAGAAAA GTGCTGGAAA TCCTGCAGGC AAAGGATAGC 
AAAATACAGG AACTTGAACA GAGTAATAAA GAGAATACGC AAGAAAAGGA AAAACAGACA 
GCAGTGATTT TAAAAAATCT AGAGCAGGAA AAGGAGGGGT TGAGTAAGAG ATGTGCTGAC 
CTGCTCAATG ACCTGGAGAA AGCAAGGAAG CAGGAGGCAC AGTGGAAAGC AGACAAATCT 
GGCATTGATG GCAAAATAAA GGATATGGAA GTTGACTTGA AAGAAGCAAG AAAACAGATG 
GAGGCTATGC ACAACAAAAT AAATGGGCTG TCATCACAGG TCATTGTAAA GCAGACAGAG 
TTGGCACAGA GAGAAACTGA TTTGACCAGG CTGAGGAAGG AGTTGCAAGA ACTGCAAAAT 
CTATACAAGC AAAGCAATGA ACATGCATCA CAGCAAGCTG AGCTGATCCA ACAGCTTCAG 
GCTCTCAATT TGGATACTCA AAAGGTACTA AGAAATCAGG AAGATGCTCA CACCACTGAA 
ACAATCTCAT ACCAGAAGCT TTATAATGAC TTGAATGTCT GTTATGATAT GGCAAAGTCC 
AGTGAGGCTC AACTCCGGCA GAGTAATATT GCTCTCACAG ACCAGATACA TCAAAAGGAA 
CAGCAGATTA GCCAGCTTCA AGTGAAACTG CAAGAAGCCC TCAGTGCATT ACCCAAGTCA 
CCTCAAGAAC CTCAGGACTG TGAACATCCA TCCTTGGTGG AACTGGAACT CATAATAGCT 
TCTCAGAAAT CAGAAATCAA ATTTCTACAG GACAAGCTTA AAACAACAGA CCTTCAGTTA 
ACTCGAGTAA ATAATTGTGG TGCCGATGCA CTTGAGAGCA GCATCCTGAG GGCTGGGAGA 
AAGCACGAGG TGCAACCTGC GAAACGTTCA AGGTCTCTGT CCCCAAAAGG ATTTTCCAAA 
GAGTCAGAAG AGTTAAAGAA GCTTAAGATA GCAGAAAAGA GAATTGAGCA TTTAGAGAAA 
GCACTCCAGA TTAAGATGCA GGAAAATGCT GAAATCCGAA AAGCTCATGA AAGACGAAGA 
GAGAGGCTAC TGACATTGCA AAGTAATTAT AGGGAAGTTA AACAACAGAT AGAGCAATCT 
GAAGAGTATG CCAGGGGAAA AGAAAATAAA AAGCGAAACC AGCGTGCAGA TCCAAGGCAA 
CTTCGCCATG AAGACTCTGA TGCAGTGTGG AATGAGCTTG CTCACTTTAA GAGGCAAAAC 
AAAAAGCTTC ATGTTGAAAA AATGAACTTA GAAGAGGAAC TAGATCAAAT CAAGGTGCAG 
GCATCTGCAG ACAAAGTAGC TATACAAGAA TTAAATGAGT GTCTTCAGCA AGAACGAGAA 
GAACTGCTTT ATATACTTGC TAAAGAGGAA GAGATCGTCA GCAGCACCCC GAAGAAAAAT 
AAAGATGAGG TTTTGCAACA GGCCTTGCAC AAGGCAAATC GTTTGGAAAT ACGTCTATGT 
GGGTTAGAAG CAGAAGTTAC AAAATTAAAA GGAATAAATG AAGAATTAAT TGCAGAGAAA 
GATTCTTTAA AATTATTGGT AACTAGTCTG CAAATAGATG CAGACTCTAA GGAAAGAAAA 
GTACAAAAAC TAAAGACTGA AAATATAGAA GCATATGAAG TAAGAGAGAA GTTAGAGATG 
AAGAATGCAG AACTAATTTA TGAGATCACA TCACTCAAGA TACTATTGGA AGAAGGTAAC 
AAGCTGCAAA GTGAAAACCA GGATCTCATA AGGCAAATAG AGAACCTACA ACGTGCTCTG 
GACCAGGTTA GAACAGTCCC TGTTCTGGCC ACTAGAGAGT TGAACAGTAG ACAAAGAAGT 
AAAAAGCATA CAGTAAACAA GGTTAAACAA AGAGCAGTCA AGAAGATCTC TTTAAGACGT 
CACCAAGCTT TTCTTAATCA GTCTATCAAG GTTATGAGCA GTGTGTTTGA GAACTTCAGC 
AGAGACGGAT GGGAAGATGT TATTGAAGAC AGTGATTCAG AGTCCCAAAC TTTGGGGAGT 
GAGAAAGAAG GAATTGTTAA AACAGCCAAG TCAGAAAATT TATGTATGGA TTACACAAAC 
TCAGCTGATG AAAACACTTG TTCTGAGTAT ACTCATTCAG GGTGTGAAGA TGCAAGTGAT 
GAACTGACCT ATGGCAAGCA GAATTATCTG CATGAGAGTG CAGCAAAGAA AAGAAACATT 
ACAAAATGTT GCACCAGGCT CCATCCACCT GCTGTAAAAT CAGGACACCG AGAGAAGAGA 
AACAAAATCC TGGCATACAG GCATATTTCC TCTGTGTCAT CGTTAAGGCA AAGAGTTGCA 
TCCTTACAGC ACCAGGTGTC CGTGTTACAA AAAGGAAAGC AAGCAGCTAG CGCTTGTGCA 
GATGAGCTAA AGAAAGAGAA GATAAAACTA ACAGCTGAAC TGCATCTGGC ACAGCAAAGA 
CTTCAAATCA GCAAGCAGAT GGCAAAGAAA TTGTCTGCAG AACTTACTGA ACTTCATCGG 
GAAAGAGAGT ATTTGGAAAG ATCTGCAGAG CAGATGAAGG AACAGCTGAA TCTAGCCAAA 
AGATCATCAG AAACGCAGCG CCTTTTGACC TCAGATCAGT CTCCAGTCAC ACAATTAACT 
GAATCAAAGA GTTCAGACCT CGAAATAAAG CAGCTCCACA CTAAACTTAA GAATATGACT 
GTTGAAATAA CGAAACAGAC CACAACGATT AAATATTTGA AAAATGAAGT CCAGGAAAAA 
GAGGACCGTA TTCGGGATCT GCAGGAAAGG GTTGCACGAA TGGAAAGGGA TGTCAACATG 
AAAAGGCATT TGATAGAGGA TCAGAAAACT CGCCTGAAAA CCTACCAGGA AAACGAGAGA 
CCTAGCAAAG AAATGTTAGA AAGTCTTGAA AGGAAGGTGA AGGCATTGAC AGAAGAATGT 
TCTCACAAGA AGACATCAAT TGATTCATTA AAGCAGAGGC TTAGTGTAGC TACAAAAGAA 
AAGGCACAGT ATGAGCAGAT GTACCACAAA GTAAAAGAAG ACATGGAAAA AAAGACTGTT 
AAGGTCAGCA ACCTTGAGAC TAAAGTGACA GAATCTGAGC ATGCCATATC TAAACTGGAA 
AACACAGCCA CTCAGCAACT TCACAATTTA GCGGCGCAAA GTGAACAAGC TTTAGAAGTC 
ATTCAGAATA AACTGCTGTT GGTTACCAGA AGGGCAGAGG AGCTTGCCAT CTTTGTAAAG 
AGTCTAGCTA AAGAGCTGCT GTTAAATATA GAAGAAATCA GAAAGGAAAT CAGGCAGGCA 
AACTTACTTC AAGAAGCAAA CAATGGTCCA TCCAAAAAAT CTGTTCACAA GGCACAATCT 
CTTGCAGCCT CTATCCTCAA CATATCATGC ACTGACCTGG ATGAGATATT AGATGTCCAG 
GATGAAGAGG AAGAAGCAAA CATAAAGCGG TTGGTGGAAT CTGATAAAAA ATGGGCGAAC 
CACATAATAA AGCTTCTGGA AGGACAGCTT CCTTTTGCAT CCAGCCTCAT GAAAGCCGTG 
CAGGAAAAAC TTAATGAGAA GGCTAGACTT ATGGAAGAAT ACACACTGCT GAAGAGACAT 
ATCTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cntln cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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