cntln cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cntln
Entrez Gene ID	799510
Full Name	centlein, centrosomal protein
Synonyms	si:ch211-2i17.1,si:dkeyp-9c8.1
General protein information	Preferred Names centlein, centrosomal protein Names centlein centlein, centrosomal protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001922094.5	XP_001922129.3	centlein
XM_021473911.1	XP_021329586.1	centlein isoform X3
XM_001922094.6	XP_001922129.3	centlein isoform X1
XM_021471204.1	XP_021326879.1	centlein isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CNTLN	NP_060208.2
Pan troglodytes (chimpanzee)	CNTLN	XP_003312056.1
Rattus norvegicus (Norway rat)	Cntln	NP_001107874.1
Gallus gallus (chicken)	C9ORF39	XP_001232685.3
Danio rerio (zebrafish)	si:ch211-2i17.1	XP_005159969.1
Bos taurus (cattle)	CNTLN	NP_001179650.1
Macaca mulatta (Rhesus monkey)	CNTLN	XP_001102815.2
Canis lupus familiaris (dog)	CNTLN	XP_538669.3
Mus musculus (house mouse)	Cntln	NP_780484.2
Xenopus tropicalis (tropical clawed frog)	cntln	XP_004910922.1

Gene Ontology

The following cntln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cntln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa05800	XM_001922094.6 Latest version!	Danio rerio centlein, centrosomal protein (cntln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa144258	XM_021471204.1 Latest version!	Danio rerio centlein, centrosomal protein (cntln), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa05800	XM_001922094.5	Danio rerio centlein, centrosomal protein (cntln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cntln ( XM_001922094.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001922129.3 Danio rerio centlein, centrosomal protein (cntln), mRNA.

Price & Availability↑

CloneID	ODa05800
Clone ID Related Accession (Same CDS sequence)	XM_001922094.5 , XM_001922094.6
Accession Version	XM_001922094.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	centlein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001922094.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAGACAATGA ACGTGTTTTA CTTCTTAAGG AAAAGCTGAT GAGTTTATCT 
GAAGAACTTG CTCAGTGCCA GGCAGACAAG GAGTTTGTGT GGTCACTTTG GAAGCGGCTT 
CAGGTGGCCA GTCCTGATCT CACCCAGGCT GTGAGTCTTG CTGTGGAAAG AGAGAAGCAG 
AAGGCTGAGA ACAAGGACAG AAAAGTTCTG GAGATCCTTC AAGCAAAAGA CTACAGGATC 
CAAGAGCTGG AGCAGAAAGT GACGGCAGAA GAGCAGGAGA TAAACAAGCG AGTCCAGCAG 
TTGCGTGGCA GTGAAGAGGA GTGCACTCTA ATGAAGAAAG AACTGGCAGC TCTGCAGCAC 
AGACTGGGGA ATAAGAGTCG ACAACTTAAG GAAGCGAGGA GGCAAGCGCA GGAGTGTGAG 
GAGGAGGGCA GGCGTGCCCT ACAGGAAGCC AGGCAACAAT TAGATGCTCA GCACTCGGCC 
TCTCAGGCAG AGCTGGAGAG ATTGCGGGAC CAAGAGGCCG TCAGCAGTTC CAGAGTCAAG 
AATTTGGAAG AGGAAGTGTT CAGAAGCAGA CAGGATTTGC TAGAGGCTCA GAGCCGCTCA 
ACTGCTTTGT CCCTTCAGCT ATCCTCAGTA GAACGAGAAA GAGCTGACAA CGAACAACAA 
CTCTGTCAAA TCAAGCGTGA GCTCCAGGAC CTGCAGTGTC TGTACAAGCA GAGCGTAGAA 
CATGCAGGAG AACAGGCCCA TCTTATTCAG CAGCTGGAGG GTCTTAACCT GGACACGCAA 
AGAGTCCTGC GCAGCCAGGA GGAGGCCCAC ACAGCGGACA CCGTCTCTTA CCAGAAGCTC 
TACAGCGAGC TGAGCGTGTC ATACCAGGCC CTTTGGCACA GCCAGGAACA GCTCCACCAG 
CGGGAAGCAG CCCTCAGCAT GCAGCTCAGC CAGAGAGATC AGCAGATCCA AGAACTCCAG 
GCACAGCTGC AGAGTTTCAC AGCAGCCGCG GGGCACTGTG AGGACTCTGG GTCTCCAGGT 
GGCAGCAGCA GTGACGGAGC TGACAGTCTG GAGAAAAGGA TTCAGCAACT GCAGGAATTA 
CTCGCTTTTA AGACTCAGGA GAATGAGGAT CTTCGGAAAG CCCATGCCAA ACATCATGAT 
CGTTTACGCG TTATTCAGAC CAACTACAAA ACAGTGACAG AGCGACTGAA AGAAGCTGAA 
GATACTCATG ACTTGGTGAA GCACAAAGTA CAATTTCCAC AGGACAATGA GGGTTTATGG 
AAAGAACTGT TTTTCTATAA GGAGGAGAAC AGGAAGCTAC TCGCTGACAA AGCTAATATG 
GAGGAGGAAC TAAACGCAGC CCTGGACAGA ATTGCAGCAC AGGAGATCCA AGAGGATCGG 
CGGGAGGCAG CGCAAGCAGA GCTTCTGTTT CGGGAAGTGG CGGAGGAGGA GGTCACAAGG 
AGCAGCACCC CCATCAAGAC AACCATGCGA AGAGTGGAAG AGACTTTTAA GAAGATCGAG 
CAGCTACAAA GTCAAATGGT CTCTCTGGAG AGTGAGATTT TGCGGTTACG AGAAGACAAC 
CGGGCACTGG CCGGTGAACG GGGTGATCTG CAGGCCAGGG TTCAGATGCT GCAGCAAAGC 
AAGGAGGCAG AAGAGGCCCG GGCCAAAGAC GAACGCAACC GACTGATTTC CCAGATGCAG 
AGCTTTGAGA GAGAGGCAAA GGAGGCTGCG AAAGCTGCTG CCCAGGCCAG ACGCAGGCTG 
CTCAAGCTTC GACAGGAACT GGGCGTCCTC CGAGCAGAGA GAGATTTCCA TCGGTCCTCT 
GCTAGACGCA AGGTGAACAA CAAAGCCCCC AGGGTGGATG CCTCCAGCAG AACACACGTG 
CTTTCGACAG AGAACAGGAT GGACAGTCCC GCCAAGGACG ACTGGGAGGA CATGAGCGCA 
GACAGTGAGA GTGAAGATTT CTCTGACAGC CTGGAAAGTC GATATTCTGC TCGGACTCGT 
CAAGCTTCTC GAAAATCGAA GGGCTGCAGG TACATGCTGA TCTGCCCTAT AGACAGACCT 
AGAGAGGAAT CTCAAACCAG AAACATCCAA TTCCAAGGTG CCTCAATGCA GAAGATGAGG 
AGGAGAAAAA CATCAAAGCC CCATTCTGCT TTACGACAGC ATCTTCTGTC CCTCCAGCAG 
CAAGTGGCAG CACTACAGGC AGACAGACAA GCAGCACAGC AGCTTGCAAC TGAGCATAAC 
CACCGACAAG TCCAAACACA AGTGCAGATT GACTCTCTTC TCCAGCAACT CAATGCTAGC 
AAGCAGCTTT CAAAGAAGCA GGCATGCGAG TTGGCTGGTC TGGAGCAGCA GAAAGCATCT 
TTGCAGATGG AGTTAGAGCA GTGGAGACGT ATACGGCAGC AGACGTCACT GAACCCCCCC 
GCTTCCGAGC CGGAACGCCT GCAGGCCCAA GTCAAACAGC TCACTCAACG ACTCAAGAGT 
GCCAGCAGTG AGATGAGCAA ACACACTGCT GCTAACAAGA GCCTGAGGGC TCAGTTAGAT 
GAAAGAGATC AGACACTGAA GGAACTGCAC GAGAGGATTG GTGTTTTAGA GAGGGATGTA 
ACGATGAAAA GGCAGCTGGT GGAAGATCTC AGGAGCAGAC TAAAGATATG CCAGGACTCT 
GAGAGGAGCC ACAAAACAGC GACTGAAGAC TTGGAGAAGA AAATAAAATC ACTCTCTGAT 
GAGTCAACTA ACCGGAAAGC TCTTGTCGAC TCTCTCAAGA GAAGATTGAC AGTTGCCACT 
GCAGAAAAAA CTCAGCAGGA GACAAGCAGC CGCAAACTCA AAGAGGAGCT TCAGAAAAAG 
GAGCAGAGAG TGAGCGTCCT GCAGGCACGG ATAGGCGAGC GTGATCAGGC CATGACTGAG 
TTGGAACAGA CGGCGTCTCA ACAAATGCAT GCTTTGACCG AGCAGAGCTC TCAGACTCTG 
GAATCTCTGC AGAGAAAACT AACCCTCGCC GAGTCTCAGC TGCGCCAGTT TCACACATTC 
ATTCAGGCTC TGGCTAATGA ATTGGCACAG GATGTGCAGG ACACTAAAGC TCGTTTGAGG 
AGAAAGAAAA AGAGTACAAA AGAAGGAAAC AAAATGTCCA AGTGCTCACT GGTGAAAGCT 
GCATCAATCC TCAACATGTC AGAAACAGAC CTGGTGGACA TGCTGGATAC AGATGAGTGT 
GAGGAGGCGT TTGTGGGTCG TGGGCCAGAC ACAGATTGGA CTGAACATGT TCAGCACATC 
CTTCAGCAGC AGATCCCCTC TGCAGGCCTG CTGATGGACG CCATGCTGGT GAAGATGAAG 
GAGGGCAAAG TGCTGACAGA GGAACTGGCT GCACTTACAG CTGCAGTCAG CAAAAACGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922129.3
CDS	84..3446
Translation	MDSEDNERVLLLKEKLMSLSEELAQCQADKEFVWSLWKRLQVASPDLTQAVSLAVEREKQKAENKDRKVLEILQAKDYRIQELEQKVTAEEQEINKRVQQLRGSEEECTLMKKELAALQHRLGNKSRQLKEARRQAQECEEEGRRALQEARQQLDAQHSASQAELERLRDQEAVSSSRVKNLEEEVFRSRQDLLEAQSRSTALSLQLSSVERERADNEQQLCQIKRELQDLQCLYKQSVEHAGEQAHLIQQLEGLNLDTQRVLRSQEEAHTADTVSYQKLYSELSVSYQALWHSQEQLHQREAALSMQLSQRDQQIQELQAQLQSFTAAAGHCEDSGSPGGSSSDGADSLEKRIQQLQELLAFKTQENEDLRKAHAKHHDRLRVIQTNYKTVTERLKEAEDTHDLVKHKVQFPQDNEGLWKELFFYKEENRKLLADKANMEEELNAALDRIAAQEIQEDRREAAQAELLFREVAEEEVTRSSTPIKTTMRRVEETFKKIEQLQSQMVSLESEILRLREDNRALAGERGDLQARVQMLQQSKEAEEARAKDERNRLISQMQSFEREAKEAAKAAAQARRRLLKLRQELGVLRAERDFHRSSARRKVNNKAPRVDASSRTHVLSTENRMDSPAKDDWEDMSADSESEDFSDSLESRYSARTRQASRKSKGCRYMLICPIDRPREESQTRNIQFQGASMQKMRRRKTSKPHSALRQHLLSLQQQVAALQADRQAAQQLATEHNHRQVQTQVQIDSLLQQLNASKQLSKKQACELAGLEQQKASLQMELEQWRRIRQQTSLNPPASEPERLQAQVKQLTQRLKSASSEMSKHTAANKSLRAQLDERDQTLKELHERIGVLERDVTMKRQLVEDLRSRLKICQDSERSHKTATEDLEKKIKSLSDESTNRKALVDSLKRRLTVATAEKTQQETSSRKLKEELQKKEQRVSVLQARIGERDQAMTELEQTASQQMHALTEQSSQTLESLQRKLTLAESQLRQFHTFIQALANELAQDVQDTKARLRRKKKSTKEGNKMSKCSLVKAASILNMSETDLVDMLDTDECEEAFVGRGPDTDWTEHVQHILQQQIPSAGLLMDAMLVKMKEGKVLTEELAALTAAVSKNA

Target ORF information:

RefSeq Version	XM_001922094.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio centlein, centrosomal protein (cntln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922094.5

ORF Insert Sequence:

ATGGATTCCG AAGACAATGA ACGTGTTTTA CTTCTTAAGG AAAAGCTGAT GAGTTTATCT 
GAAGAACTTG CTCAGTGCCA GGCAGACAAG GAGTTTGTGT GGTCACTTTG GAAGCGGCTT 
CAGGTGGCCA GTCCTGATCT CACCCAGGCT GTGAGTCTTG CTGTGGAAAG AGAGAAGCAG 
AAGGCTGAGA ACAAGGACAG AAAAGTTCTG GAGATCCTTC AAGCAAAAGA CTACAGGATC 
CAAGAGCTGG AGCAGAAAGT GACGGCAGAA GAGCAGGAGA TAAACAAGCG AGTCCAGCAG 
TTGCGTGGCA GTGAAGAGGA GTGCACTCTA ATGAAGAAAG AACTGGCAGC TCTGCAGCAC 
AGACTGGGGA ATAAGAGTCG ACAACTTAAG GAAGCGAGGA GGCAAGCGCA GGAGTGTGAG 
GAGGAGGGCA GGCGTGCCCT ACAGGAAGCC AGGCAACAAT TAGATGCTCA GCACTCGGCC 
TCTCAGGCAG AGCTGGAGAG ATTGCGGGAC CAAGAGGCCG TCAGCAGTTC CAGAGTCAAG 
AATTTGGAAG AGGAAGTGTT CAGAAGCAGA CAGGATTTGC TAGAGGCTCA GAGCCGCTCA 
ACTGCTTTGT CCCTTCAGCT ATCCTCAGTA GAACGAGAAA GAGCTGACAA CGAACAACAA 
CTCTGTCAAA TCAAGCGTGA GCTCCAGGAC CTGCAGTGTC TGTACAAGCA GAGCGTAGAA 
CATGCAGGAG AACAGGCCCA TCTTATTCAG CAGCTGGAGG GTCTTAACCT GGACACGCAA 
AGAGTCCTGC GCAGCCAGGA GGAGGCCCAC ACAGCGGACA CCGTCTCTTA CCAGAAGCTC 
TACAGCGAGC TGAGCGTGTC ATACCAGGCC CTTTGGCACA GCCAGGAACA GCTCCACCAG 
CGGGAAGCAG CCCTCAGCAT GCAGCTCAGC CAGAGAGATC AGCAGATCCA AGAACTCCAG 
GCACAGCTGC AGAGTTTCAC AGCAGCCGCG GGGCACTGTG AGGACTCTGG GTCTCCAGGT 
GGCAGCAGCA GTGACGGAGC TGACAGTCTG GAGAAAAGGA TTCAGCAACT GCAGGAATTA 
CTCGCTTTTA AGACTCAGGA GAATGAGGAT CTTCGGAAAG CCCATGCCAA ACATCATGAT 
CGTTTACGCG TTATTCAGAC CAACTACAAA ACAGTGACAG AGCGACTGAA AGAAGCTGAA 
GATACTCATG ACTTGGTGAA GCACAAAGTA CAATTTCCAC AGGACAATGA GGGTTTATGG 
AAAGAACTGT TTTTCTATAA GGAGGAGAAC AGGAAGCTAC TCGCTGACAA AGCTAATATG 
GAGGAGGAAC TAAACGCAGC CCTGGACAGA ATTGCAGCAC AGGAGATCCA AGAGGATCGG 
CGGGAGGCAG CGCAAGCAGA GCTTCTGTTT CGGGAAGTGG CGGAGGAGGA GGTCACAAGG 
AGCAGCACCC CCATCAAGAC AACCATGCGA AGAGTGGAAG AGACTTTTAA GAAGATCGAG 
CAGCTACAAA GTCAAATGGT CTCTCTGGAG AGTGAGATTT TGCGGTTACG AGAAGACAAC 
CGGGCACTGG CCGGTGAACG GGGTGATCTG CAGGCCAGGG TTCAGATGCT GCAGCAAAGC 
AAGGAGGCAG AAGAGGCCCG GGCCAAAGAC GAACGCAACC GACTGATTTC CCAGATGCAG 
AGCTTTGAGA GAGAGGCAAA GGAGGCTGCG AAAGCTGCTG CCCAGGCCAG ACGCAGGCTG 
CTCAAGCTTC GACAGGAACT GGGCGTCCTC CGAGCAGAGA GAGATTTCCA TCGGTCCTCT 
GCTAGACGCA AGGTGAACAA CAAAGCCCCC AGGGTGGATG CCTCCAGCAG AACACACGTG 
CTTTCGACAG AGAACAGGAT GGACAGTCCC GCCAAGGACG ACTGGGAGGA CATGAGCGCA 
GACAGTGAGA GTGAAGATTT CTCTGACAGC CTGGAAAGTC GATATTCTGC TCGGACTCGT 
CAAGCTTCTC GAAAATCGAA GGGCTGCAGG TACATGCTGA TCTGCCCTAT AGACAGACCT 
AGAGAGGAAT CTCAAACCAG AAACATCCAA TTCCAAGGTG CCTCAATGCA GAAGATGAGG 
AGGAGAAAAA CATCAAAGCC CCATTCTGCT TTACGACAGC ATCTTCTGTC CCTCCAGCAG 
CAAGTGGCAG CACTACAGGC AGACAGACAA GCAGCACAGC AGCTTGCAAC TGAGCATAAC 
CACCGACAAG TCCAAACACA AGTGCAGATT GACTCTCTTC TCCAGCAACT CAATGCTAGC 
AAGCAGCTTT CAAAGAAGCA GGCATGCGAG TTGGCTGGTC TGGAGCAGCA GAAAGCATCT 
TTGCAGATGG AGTTAGAGCA GTGGAGACGT ATACGGCAGC AGACGTCACT GAACCCCCCC 
GCTTCCGAGC CGGAACGCCT GCAGGCCCAA GTCAAACAGC TCACTCAACG ACTCAAGAGT 
GCCAGCAGTG AGATGAGCAA ACACACTGCT GCTAACAAGA GCCTGAGGGC TCAGTTAGAT 
GAAAGAGATC AGACACTGAA GGAACTGCAC GAGAGGATTG GTGTTTTAGA GAGGGATGTA 
ACGATGAAAA GGCAGCTGGT GGAAGATCTC AGGAGCAGAC TAAAGATATG CCAGGACTCT 
GAGAGGAGCC ACAAAACAGC GACTGAAGAC TTGGAGAAGA AAATAAAATC ACTCTCTGAT 
GAGTCAACTA ACCGGAAAGC TCTTGTCGAC TCTCTCAAGA GAAGATTGAC AGTTGCCACT 
GCAGAAAAAA CTCAGCAGGA GACAAGCAGC CGCAAACTCA AAGAGGAGCT TCAGAAAAAG 
GAGCAGAGAG TGAGCGTCCT GCAGGCACGG ATAGGCGAGC GTGATCAGGC CATGACTGAG 
TTGGAACAGA CGGCGTCTCA ACAAATGCAT GCTTTGACCG AGCAGAGCTC TCAGACTCTG 
GAATCTCTGC AGAGAAAACT AACCCTCGCC GAGTCTCAGC TGCGCCAGTT TCACACATTC 
ATTCAGGCTC TGGCTAATGA ATTGGCACAG GATGTGCAGG ACACTAAAGC TCGTTTGAGG 
AGAAAGAAAA AGAGTACAAA AGAAGGAAAC AAAATGTCCA AGTGCTCACT GGTGAAAGCT 
GCATCAATCC TCAACATGTC AGAAACAGAC CTGGTGGACA TGCTGGATAC AGATGAGTGT 
GAGGAGGCGT TTGTGGGTCG TGGGCCAGAC ACAGATTGGA CTGAACATGT TCAGCACATC 
CTTCAGCAGC AGATCCCCTC TGCAGGCCTG CTGATGGACG CCATGCTGGT GAAGATGAAG 
GAGGGCAAAG TGCTGACAGA GGAACTGGCT GCACTTACAG CTGCAGTCAG CAAAAACGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cntln ( XM_001922094.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001922129.3 Danio rerio centlein, centrosomal protein (cntln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05800
Clone ID Related Accession (Same CDS sequence)	XM_001922094.5 , XM_001922094.6
Accession Version	XM_001922094.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	centlein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001922094.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAGACAATGA ACGTGTTTTA CTTCTTAAGG AAAAGCTGAT GAGTTTATCT 
GAAGAACTTG CTCAGTGCCA GGCAGACAAG GAGTTTGTGT GGTCACTTTG GAAGCGGCTT 
CAGGTGGCCA GTCCTGATCT CACCCAGGCT GTGAGTCTTG CTGTGGAAAG AGAGAAGCAG 
AAGGCTGAGA ACAAGGACAG AAAAGTTCTG GAGATCCTTC AAGCAAAAGA CTACAGGATC 
CAAGAGCTGG AGCAGAAAGT GACGGCAGAA GAGCAGGAGA TAAACAAGCG AGTCCAGCAG 
TTGCGTGGCA GTGAAGAGGA GTGCACTCTA ATGAAGAAAG AACTGGCAGC TCTGCAGCAC 
AGACTGGGGA ATAAGAGTCG ACAACTTAAG GAAGCGAGGA GGCAAGCGCA GGAGTGTGAG 
GAGGAGGGCA GGCGTGCCCT ACAGGAAGCC AGGCAACAAT TAGATGCTCA GCACTCGGCC 
TCTCAGGCAG AGCTGGAGAG ATTGCGGGAC CAAGAGGCCG TCAGCAGTTC CAGAGTCAAG 
AATTTGGAAG AGGAAGTGTT CAGAAGCAGA CAGGATTTGC TAGAGGCTCA GAGCCGCTCA 
ACTGCTTTGT CCCTTCAGCT ATCCTCAGTA GAACGAGAAA GAGCTGACAA CGAACAACAA 
CTCTGTCAAA TCAAGCGTGA GCTCCAGGAC CTGCAGTGTC TGTACAAGCA GAGCGTAGAA 
CATGCAGGAG AACAGGCCCA TCTTATTCAG CAGCTGGAGG GTCTTAACCT GGACACGCAA 
AGAGTCCTGC GCAGCCAGGA GGAGGCCCAC ACAGCGGACA CCGTCTCTTA CCAGAAGCTC 
TACAGCGAGC TGAGCGTGTC ATACCAGGCC CTTTGGCACA GCCAGGAACA GCTCCACCAG 
CGGGAAGCAG CCCTCAGCAT GCAGCTCAGC CAGAGAGATC AGCAGATCCA AGAACTCCAG 
GCACAGCTGC AGAGTTTCAC AGCAGCCGCG GGGCACTGTG AGGACTCTGG GTCTCCAGGT 
GGCAGCAGCA GTGACGGAGC TGACAGTCTG GAGAAAAGGA TTCAGCAACT GCAGGAATTA 
CTCGCTTTTA AGACTCAGGA GAATGAGGAT CTTCGGAAAG CCCATGCCAA ACATCATGAT 
CGTTTACGCG TTATTCAGAC CAACTACAAA ACAGTGACAG AGCGACTGAA AGAAGCTGAA 
GATACTCATG ACTTGGTGAA GCACAAAGTA CAATTTCCAC AGGACAATGA GGGTTTATGG 
AAAGAACTGT TTTTCTATAA GGAGGAGAAC AGGAAGCTAC TCGCTGACAA AGCTAATATG 
GAGGAGGAAC TAAACGCAGC CCTGGACAGA ATTGCAGCAC AGGAGATCCA AGAGGATCGG 
CGGGAGGCAG CGCAAGCAGA GCTTCTGTTT CGGGAAGTGG CGGAGGAGGA GGTCACAAGG 
AGCAGCACCC CCATCAAGAC AACCATGCGA AGAGTGGAAG AGACTTTTAA GAAGATCGAG 
CAGCTACAAA GTCAAATGGT CTCTCTGGAG AGTGAGATTT TGCGGTTACG AGAAGACAAC 
CGGGCACTGG CCGGTGAACG GGGTGATCTG CAGGCCAGGG TTCAGATGCT GCAGCAAAGC 
AAGGAGGCAG AAGAGGCCCG GGCCAAAGAC GAACGCAACC GACTGATTTC CCAGATGCAG 
AGCTTTGAGA GAGAGGCAAA GGAGGCTGCG AAAGCTGCTG CCCAGGCCAG ACGCAGGCTG 
CTCAAGCTTC GACAGGAACT GGGCGTCCTC CGAGCAGAGA GAGATTTCCA TCGGTCCTCT 
GCTAGACGCA AGGTGAACAA CAAAGCCCCC AGGGTGGATG CCTCCAGCAG AACACACGTG 
CTTTCGACAG AGAACAGGAT GGACAGTCCC GCCAAGGACG ACTGGGAGGA CATGAGCGCA 
GACAGTGAGA GTGAAGATTT CTCTGACAGC CTGGAAAGTC GATATTCTGC TCGGACTCGT 
CAAGCTTCTC GAAAATCGAA GGGCTGCAGG TACATGCTGA TCTGCCCTAT AGACAGACCT 
AGAGAGGAAT CTCAAACCAG AAACATCCAA TTCCAAGGTG CCTCAATGCA GAAGATGAGG 
AGGAGAAAAA CATCAAAGCC CCATTCTGCT TTACGACAGC ATCTTCTGTC CCTCCAGCAG 
CAAGTGGCAG CACTACAGGC AGACAGACAA GCAGCACAGC AGCTTGCAAC TGAGCATAAC 
CACCGACAAG TCCAAACACA AGTGCAGATT GACTCTCTTC TCCAGCAACT CAATGCTAGC 
AAGCAGCTTT CAAAGAAGCA GGCATGCGAG TTGGCTGGTC TGGAGCAGCA GAAAGCATCT 
TTGCAGATGG AGTTAGAGCA GTGGAGACGT ATACGGCAGC AGACGTCACT GAACCCCCCC 
GCTTCCGAGC CGGAACGCCT GCAGGCCCAA GTCAAACAGC TCACTCAACG ACTCAAGAGT 
GCCAGCAGTG AGATGAGCAA ACACACTGCT GCTAACAAGA GCCTGAGGGC TCAGTTAGAT 
GAAAGAGATC AGACACTGAA GGAACTGCAC GAGAGGATTG GTGTTTTAGA GAGGGATGTA 
ACGATGAAAA GGCAGCTGGT GGAAGATCTC AGGAGCAGAC TAAAGATATG CCAGGACTCT 
GAGAGGAGCC ACAAAACAGC GACTGAAGAC TTGGAGAAGA AAATAAAATC ACTCTCTGAT 
GAGTCAACTA ACCGGAAAGC TCTTGTCGAC TCTCTCAAGA GAAGATTGAC AGTTGCCACT 
GCAGAAAAAA CTCAGCAGGA GACAAGCAGC CGCAAACTCA AAGAGGAGCT TCAGAAAAAG 
GAGCAGAGAG TGAGCGTCCT GCAGGCACGG ATAGGCGAGC GTGATCAGGC CATGACTGAG 
TTGGAACAGA CGGCGTCTCA ACAAATGCAT GCTTTGACCG AGCAGAGCTC TCAGACTCTG 
GAATCTCTGC AGAGAAAACT AACCCTCGCC GAGTCTCAGC TGCGCCAGTT TCACACATTC 
ATTCAGGCTC TGGCTAATGA ATTGGCACAG GATGTGCAGG ACACTAAAGC TCGTTTGAGG 
AGAAAGAAAA AGAGTACAAA AGAAGGAAAC AAAATGTCCA AGTGCTCACT GGTGAAAGCT 
GCATCAATCC TCAACATGTC AGAAACAGAC CTGGTGGACA TGCTGGATAC AGATGAGTGT 
GAGGAGGCGT TTGTGGGTCG TGGGCCAGAC ACAGATTGGA CTGAACATGT TCAGCACATC 
CTTCAGCAGC AGATCCCCTC TGCAGGCCTG CTGATGGACG CCATGCTGGT GAAGATGAAG 
GAGGGCAAAG TGCTGACAGA GGAACTGGCT GCACTTACAG CTGCAGTCAG CAAAAACGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001922129.3
CDS	84..3446
Translation	MDSEDNERVLLLKEKLMSLSEELAQCQADKEFVWSLWKRLQVASPDLTQAVSLAVEREKQKAENKDRKVLEILQAKDYRIQELEQKVTAEEQEINKRVQQLRGSEEECTLMKKELAALQHRLGNKSRQLKEARRQAQECEEEGRRALQEARQQLDAQHSASQAELERLRDQEAVSSSRVKNLEEEVFRSRQDLLEAQSRSTALSLQLSSVERERADNEQQLCQIKRELQDLQCLYKQSVEHAGEQAHLIQQLEGLNLDTQRVLRSQEEAHTADTVSYQKLYSELSVSYQALWHSQEQLHQREAALSMQLSQRDQQIQELQAQLQSFTAAAGHCEDSGSPGGSSSDGADSLEKRIQQLQELLAFKTQENEDLRKAHAKHHDRLRVIQTNYKTVTERLKEAEDTHDLVKHKVQFPQDNEGLWKELFFYKEENRKLLADKANMEEELNAALDRIAAQEIQEDRREAAQAELLFREVAEEEVTRSSTPIKTTMRRVEETFKKIEQLQSQMVSLESEILRLREDNRALAGERGDLQARVQMLQQSKEAEEARAKDERNRLISQMQSFEREAKEAAKAAAQARRRLLKLRQELGVLRAERDFHRSSARRKVNNKAPRVDASSRTHVLSTENRMDSPAKDDWEDMSADSESEDFSDSLESRYSARTRQASRKSKGCRYMLICPIDRPREESQTRNIQFQGASMQKMRRRKTSKPHSALRQHLLSLQQQVAALQADRQAAQQLATEHNHRQVQTQVQIDSLLQQLNASKQLSKKQACELAGLEQQKASLQMELEQWRRIRQQTSLNPPASEPERLQAQVKQLTQRLKSASSEMSKHTAANKSLRAQLDERDQTLKELHERIGVLERDVTMKRQLVEDLRSRLKICQDSERSHKTATEDLEKKIKSLSDESTNRKALVDSLKRRLTVATAEKTQQETSSRKLKEELQKKEQRVSVLQARIGERDQAMTELEQTASQQMHALTEQSSQTLESLQRKLTLAESQLRQFHTFIQALANELAQDVQDTKARLRRKKKSTKEGNKMSKCSLVKAASILNMSETDLVDMLDTDECEEAFVGRGPDTDWTEHVQHILQQQIPSAGLLMDAMLVKMKEGKVLTEELAALTAAVSKNA

Target ORF information:

RefSeq Version	XM_001922094.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio centlein, centrosomal protein (cntln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001922094.6

ORF Insert Sequence:

ATGGATTCCG AAGACAATGA ACGTGTTTTA CTTCTTAAGG AAAAGCTGAT GAGTTTATCT 
GAAGAACTTG CTCAGTGCCA GGCAGACAAG GAGTTTGTGT GGTCACTTTG GAAGCGGCTT 
CAGGTGGCCA GTCCTGATCT CACCCAGGCT GTGAGTCTTG CTGTGGAAAG AGAGAAGCAG 
AAGGCTGAGA ACAAGGACAG AAAAGTTCTG GAGATCCTTC AAGCAAAAGA CTACAGGATC 
CAAGAGCTGG AGCAGAAAGT GACGGCAGAA GAGCAGGAGA TAAACAAGCG AGTCCAGCAG 
TTGCGTGGCA GTGAAGAGGA GTGCACTCTA ATGAAGAAAG AACTGGCAGC TCTGCAGCAC 
AGACTGGGGA ATAAGAGTCG ACAACTTAAG GAAGCGAGGA GGCAAGCGCA GGAGTGTGAG 
GAGGAGGGCA GGCGTGCCCT ACAGGAAGCC AGGCAACAAT TAGATGCTCA GCACTCGGCC 
TCTCAGGCAG AGCTGGAGAG ATTGCGGGAC CAAGAGGCCG TCAGCAGTTC CAGAGTCAAG 
AATTTGGAAG AGGAAGTGTT CAGAAGCAGA CAGGATTTGC TAGAGGCTCA GAGCCGCTCA 
ACTGCTTTGT CCCTTCAGCT ATCCTCAGTA GAACGAGAAA GAGCTGACAA CGAACAACAA 
CTCTGTCAAA TCAAGCGTGA GCTCCAGGAC CTGCAGTGTC TGTACAAGCA GAGCGTAGAA 
CATGCAGGAG AACAGGCCCA TCTTATTCAG CAGCTGGAGG GTCTTAACCT GGACACGCAA 
AGAGTCCTGC GCAGCCAGGA GGAGGCCCAC ACAGCGGACA CCGTCTCTTA CCAGAAGCTC 
TACAGCGAGC TGAGCGTGTC ATACCAGGCC CTTTGGCACA GCCAGGAACA GCTCCACCAG 
CGGGAAGCAG CCCTCAGCAT GCAGCTCAGC CAGAGAGATC AGCAGATCCA AGAACTCCAG 
GCACAGCTGC AGAGTTTCAC AGCAGCCGCG GGGCACTGTG AGGACTCTGG GTCTCCAGGT 
GGCAGCAGCA GTGACGGAGC TGACAGTCTG GAGAAAAGGA TTCAGCAACT GCAGGAATTA 
CTCGCTTTTA AGACTCAGGA GAATGAGGAT CTTCGGAAAG CCCATGCCAA ACATCATGAT 
CGTTTACGCG TTATTCAGAC CAACTACAAA ACAGTGACAG AGCGACTGAA AGAAGCTGAA 
GATACTCATG ACTTGGTGAA GCACAAAGTA CAATTTCCAC AGGACAATGA GGGTTTATGG 
AAAGAACTGT TTTTCTATAA GGAGGAGAAC AGGAAGCTAC TCGCTGACAA AGCTAATATG 
GAGGAGGAAC TAAACGCAGC CCTGGACAGA ATTGCAGCAC AGGAGATCCA AGAGGATCGG 
CGGGAGGCAG CGCAAGCAGA GCTTCTGTTT CGGGAAGTGG CGGAGGAGGA GGTCACAAGG 
AGCAGCACCC CCATCAAGAC AACCATGCGA AGAGTGGAAG AGACTTTTAA GAAGATCGAG 
CAGCTACAAA GTCAAATGGT CTCTCTGGAG AGTGAGATTT TGCGGTTACG AGAAGACAAC 
CGGGCACTGG CCGGTGAACG GGGTGATCTG CAGGCCAGGG TTCAGATGCT GCAGCAAAGC 
AAGGAGGCAG AAGAGGCCCG GGCCAAAGAC GAACGCAACC GACTGATTTC CCAGATGCAG 
AGCTTTGAGA GAGAGGCAAA GGAGGCTGCG AAAGCTGCTG CCCAGGCCAG ACGCAGGCTG 
CTCAAGCTTC GACAGGAACT GGGCGTCCTC CGAGCAGAGA GAGATTTCCA TCGGTCCTCT 
GCTAGACGCA AGGTGAACAA CAAAGCCCCC AGGGTGGATG CCTCCAGCAG AACACACGTG 
CTTTCGACAG AGAACAGGAT GGACAGTCCC GCCAAGGACG ACTGGGAGGA CATGAGCGCA 
GACAGTGAGA GTGAAGATTT CTCTGACAGC CTGGAAAGTC GATATTCTGC TCGGACTCGT 
CAAGCTTCTC GAAAATCGAA GGGCTGCAGG TACATGCTGA TCTGCCCTAT AGACAGACCT 
AGAGAGGAAT CTCAAACCAG AAACATCCAA TTCCAAGGTG CCTCAATGCA GAAGATGAGG 
AGGAGAAAAA CATCAAAGCC CCATTCTGCT TTACGACAGC ATCTTCTGTC CCTCCAGCAG 
CAAGTGGCAG CACTACAGGC AGACAGACAA GCAGCACAGC AGCTTGCAAC TGAGCATAAC 
CACCGACAAG TCCAAACACA AGTGCAGATT GACTCTCTTC TCCAGCAACT CAATGCTAGC 
AAGCAGCTTT CAAAGAAGCA GGCATGCGAG TTGGCTGGTC TGGAGCAGCA GAAAGCATCT 
TTGCAGATGG AGTTAGAGCA GTGGAGACGT ATACGGCAGC AGACGTCACT GAACCCCCCC 
GCTTCCGAGC CGGAACGCCT GCAGGCCCAA GTCAAACAGC TCACTCAACG ACTCAAGAGT 
GCCAGCAGTG AGATGAGCAA ACACACTGCT GCTAACAAGA GCCTGAGGGC TCAGTTAGAT 
GAAAGAGATC AGACACTGAA GGAACTGCAC GAGAGGATTG GTGTTTTAGA GAGGGATGTA 
ACGATGAAAA GGCAGCTGGT GGAAGATCTC AGGAGCAGAC TAAAGATATG CCAGGACTCT 
GAGAGGAGCC ACAAAACAGC GACTGAAGAC TTGGAGAAGA AAATAAAATC ACTCTCTGAT 
GAGTCAACTA ACCGGAAAGC TCTTGTCGAC TCTCTCAAGA GAAGATTGAC AGTTGCCACT 
GCAGAAAAAA CTCAGCAGGA GACAAGCAGC CGCAAACTCA AAGAGGAGCT TCAGAAAAAG 
GAGCAGAGAG TGAGCGTCCT GCAGGCACGG ATAGGCGAGC GTGATCAGGC CATGACTGAG 
TTGGAACAGA CGGCGTCTCA ACAAATGCAT GCTTTGACCG AGCAGAGCTC TCAGACTCTG 
GAATCTCTGC AGAGAAAACT AACCCTCGCC GAGTCTCAGC TGCGCCAGTT TCACACATTC 
ATTCAGGCTC TGGCTAATGA ATTGGCACAG GATGTGCAGG ACACTAAAGC TCGTTTGAGG 
AGAAAGAAAA AGAGTACAAA AGAAGGAAAC AAAATGTCCA AGTGCTCACT GGTGAAAGCT 
GCATCAATCC TCAACATGTC AGAAACAGAC CTGGTGGACA TGCTGGATAC AGATGAGTGT 
GAGGAGGCGT TTGTGGGTCG TGGGCCAGAC ACAGATTGGA CTGAACATGT TCAGCACATC 
CTTCAGCAGC AGATCCCCTC TGCAGGCCTG CTGATGGACG CCATGCTGGT GAAGATGAAG 
GAGGGCAAAG TGCTGACAGA GGAACTGGCT GCACTTACAG CTGCAGTCAG CAAAAACGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cntln ( XM_021471204.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326879.1 Danio rerio centlein, centrosomal protein (cntln), transcript variant X2, mRNA.

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CloneID	ODa144258
Clone ID Related Accession (Same CDS sequence)	XM_021471204.1
Accession Version	XM_021471204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	centlein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394485.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AAGACAATGA ACGTGTTTTA CTTCTTAAGG AAAAGCTGAT GAGTTTATCT 
GAAGAACTTG CTCAGTGCCA GGCAGACAAG GAGTTTGTGT GGTCACTTTG GAAGCGGCTT 
CAGGTGGCCA GTCCTGATCT CACCCAGGCT GTGAGTCTTG CTGTGGAAAG AGAGAAGCAG 
AAGGCTGAGA ACAAGGACAG AAAAGTTCTG GAGATCCTTC AAGCAAAAGA CTACAGGATC 
CAAGAGCTGG AGCAGAAAGT GACGGCAGAA GAGCAGGAGA TAAACAAGCG AGTCCAGCAG 
TTGCGTGGCA GTGAAGAGGA GTGCACTCTA ATGAAGAAAG AACTGGCAGC TCTGCAGCAC 
AGACTGGGGA ATAAGAGTCG ACAACTTAAG GAAGCGAGGA GGCAAGCGCA GGAGTGTGAG 
GAGGAGGGCA GGCGTGCCCT ACAGGAAGCC AGGCAACAAT TAGATGCTCA GCACTCGGCC 
TCTCAGGCAG AGCTGGAGAG ATTGCGGGAC CAAGAGGCCG TCAGCAGTTC CAGAGTCAAG 
AATTTGGAAG AGGAAGTGTT CAGAAGCAGA CAGGATTTGC TAGAGGCTCA GAGCCGCTCA 
ACTGCTTTGT CCCTTCAGCT ATCCTCAGTA GAACGAGAAA GAGCTGACAA CGAACAACAA 
CTCTGTCAAA TCAAGCGTGA GCTCCAGGAC CTGCAGTGTC TGTACAAGCA GAGCGTAGAA 
CATGCAGGAG AACAGGCCCA TCTTATTCAG CAGCTGGAGG GTCTTAACCT GGACACGCAA 
AGAGTCCTGC GCAGCCAGGA GGAGGCCCAC ACAGCGGACA CCGTCTCTTA CCAGAAGCTC 
TACAGCGAGC TGAGCGTGTC ATACCAGGCC CTTTGGCACA GCCAGGAACA GCTCCACCAG 
CGGGAAGCAG CCCTCAGCAT GCAGCTCAGC CAGAGAGATC AGCAGATCCA AGAACTCCAG 
GCACAGCTGC AGAGTTTCAC AGCAGCCGCG GGGCACTGTG AGGACTCTGG GTCTCCAGGT 
GGCAGCAGCA GTGACGGAGC TGACAGTCTG GAGAAAAGGA TTCAGCAACT GCAGGAATTA 
CTCGCTTTTA AGACTCAGGA GAATGAGGAT CTTCGGAAAG CCCATGCCAA ACATCATGAT 
CGTTTACGCG TTATTCAGAC CAACTACAAA ACAGTGACAG AGCGACTGAA AGAAGCTGAA 
GATACTCATG ACTTGGTGAA GCACAAAGTA CAATTTCCAC AGGACAGTGA GGGTTTATGG 
AAAGAACTGT TTTTCTATAA GGAGGAGAAC AGGAAGCTAC TCGCTGACAA AGCTAATATG 
GAGGAGGAAC TAAACGCAGC CCTGGACAGA ATTGCAGCAC AGGAGATCCA AGAGGATCGG 
CGGGAGGCAG CGCAAGCAGA GCTTCTGTTT CGGGAAGTGG CGGAGGAGGA GGTCACAAGG 
AGCAGCACCC CCATCAAGAC AACCATGCGA AGAGTGGAAG AGACTTTTAA GAAGATCGAG 
CAGCTACAAA GTCAAATGGT CTCTCTGGAG AGTGAGATTT TGCGGTTACG AGAAGACAAC 
CGGGCACTGG CCGGTGAACG GGGTGATCTG CAGGCCAGGG TTCAGATGCT GCAGCAAAGC 
AAGGAGGCAG AAGAGGCCCG GGCCAAAGAC GAACGCAACC GACTGATTTC CCAGATGCAG 
AGCTTTGAGA GAGAGGCAAA GGAGGCTGCG AAAGCTGCTG CCCAGGCCAG ACGCAGGCTG 
CTCAAGCTTC GACAGGAACT GGGCGTCCTC CGAGCAGAGA GAGATTTCCA TCGGTCCTCT 
GCTAGACGCA AGGTGAACAA CAAAGCCCCC AGGGTGGATG CCTCCAGCAG AACACACGTG 
CTTTCGACAG AGAACAGGAT GGACAGTCCC GCCAAGGACG ACTGGGAGGA CATGAGCGCA 
GACAGTGAGA GTGAAGATTT CTCTGACAGC CTGGAAAGTC GATATTCTGC TCGGACTCGT 
CAAGCTTCTC GAAAATCGAA GGGCTGCAGG TACATGCTGA TCTGCCCTAT AGACAGACCT 
AGAGAGGAAT CTCAAACCAG AAACATCCAA TTCCAAGGTG CCTCAATGCA GAAGATGAGG 
AGGAGAAAAA CATCAAAGCC CCATTCTGCT TTACGACAGC ATCTTCTGTC CCTCCAGCAG 
CAAGTGGCAG CACTACAGGC AGACAGACAA GCAGCACAGC AGCTTGCAAC TGAGCATAAC 
CACCGACAAG TCCAAACACA AGTGCAGATT GACTCTCTTC TCCAGCAACT CAATGCTAGC 
AAGCAGCTTT CAAAGAAGCA GGCATGCGAG TTGGCTGGTC TGGAGCAGCA GAAAGCATCT 
TTGCAGATGG AGTTAGAGCA GTGGAGACGT ATACGGCAGC AGACGTCACT GAACCCCCCC 
GCTTCCGAGC CGGAACGCCT GCAGGCCCAA GTCAAACAGC TCACTCAACG ACTCAAGAGT 
GCCAGCAGTG AGATGAGCAA ACACACTGCT GCTAACAAGA GCCTGAGGGC TCAGTTAGAT 
GAAAGAGATC AGACACTGAA GGAACTGCAC GAGAGGATTG GTGTTTTAGA GAGGGATGTA 
ACGATGAAAA GGCAGCTGGT GGAAGATCTC AGGAGCAGAC TAAAGATATG CCAGGACTCT 
GAGAGGAGCC ACAAAACAGC GACTGAAGAC TTGGAGAAGA AAATAAAATC ACTCTCTGAT 
GAGTCAACTA ACCGGAAAGC TCTTGTCGAC TCTCTCAAGA GAAGATTGAC AGTTGCCACT 
GCAGAAAAAA CTCAGCAGGA GACAAGCAGC CGCAAACTCA AAGAGGAGCT TCAGAAAAAG 
GAGCAGAGAG TGAGCGTCCT GCAGGCACGG ATAGGCGAGC GTGATCAGGC CATGACTGAG 
TTGGAACAGA CGGCGTCTCA ACAAATGCAT GCTTTGACCG AGCAGAGCTC TCAGACTCTG 
GAATCTCTGC AGAGAAAACT AACCCTCGCC GAGTCTCAGC TGCGCCAGTT TCACACATTC 
ATTCAGGTAT CTCAACTCTC CGACGTTTGC AATCAATTTA GCTTTAGGCC TTTTAAATAT 
CTATCTTATG TATTAAATTA CTGTTTAATC CTGTACATAT TACTGCTACC ACCTGGTTGT 
AATATGCATC TTAAATTTGT ATCCTATGTC CAAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326879.1
CDS	84..3242
Translation	MDSEDNERVLLLKEKLMSLSEELAQCQADKEFVWSLWKRLQVASPDLTQAVSLAVEREKQKAENKDRKVLEILQAKDYRIQELEQKVTAEEQEINKRVQQLRGSEEECTLMKKELAALQHRLGNKSRQLKEARRQAQECEEEGRRALQEARQQLDAQHSASQAELERLRDQEAVSSSRVKNLEEEVFRSRQDLLEAQSRSTALSLQLSSVERERADNEQQLCQIKRELQDLQCLYKQSVEHAGEQAHLIQQLEGLNLDTQRVLRSQEEAHTADTVSYQKLYSELSVSYQALWHSQEQLHQREAALSMQLSQRDQQIQELQAQLQSFTAAAGHCEDSGSPGGSSSDGADSLEKRIQQLQELLAFKTQENEDLRKAHAKHHDRLRVIQTNYKTVTERLKEAEDTHDLVKHKVQFPQDSEGLWKELFFYKEENRKLLADKANMEEELNAALDRIAAQEIQEDRREAAQAELLFREVAEEEVTRSSTPIKTTMRRVEETFKKIEQLQSQMVSLESEILRLREDNRALAGERGDLQARVQMLQQSKEAEEARAKDERNRLISQMQSFEREAKEAAKAAAQARRRLLKLRQELGVLRAERDFHRSSARRKVNNKAPRVDASSRTHVLSTENRMDSPAKDDWEDMSADSESEDFSDSLESRYSARTRQASRKSKGCRYMLICPIDRPREESQTRNIQFQGASMQKMRRRKTSKPHSALRQHLLSLQQQVAALQADRQAAQQLATEHNHRQVQTQVQIDSLLQQLNASKQLSKKQACELAGLEQQKASLQMELEQWRRIRQQTSLNPPASEPERLQAQVKQLTQRLKSASSEMSKHTAANKSLRAQLDERDQTLKELHERIGVLERDVTMKRQLVEDLRSRLKICQDSERSHKTATEDLEKKIKSLSDESTNRKALVDSLKRRLTVATAEKTQQETSSRKLKEELQKKEQRVSVLQARIGERDQAMTELEQTASQQMHALTEQSSQTLESLQRKLTLAESQLRQFHTFIQVSQLSDVCNQFSFRPFKYLSYVLNYCLILYILLLPPGCNMHLKFVSYVQA

Target ORF information:

RefSeq Version	XM_021471204.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio centlein, centrosomal protein (cntln), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021471204.1

ORF Insert Sequence:

ATGGATTCCG AAGACAATGA ACGTGTTTTA CTTCTTAAGG AAAAGCTGAT GAGTTTATCT 
GAAGAACTTG CTCAGTGCCA GGCAGACAAG GAGTTTGTGT GGTCACTTTG GAAGCGGCTT 
CAGGTGGCCA GTCCTGATCT CACCCAGGCT GTGAGTCTTG CTGTGGAAAG AGAGAAGCAG 
AAGGCTGAGA ACAAGGACAG AAAAGTTCTG GAGATCCTTC AAGCAAAAGA CTACAGGATC 
CAAGAGCTGG AGCAGAAAGT GACGGCAGAA GAGCAGGAGA TAAACAAGCG AGTCCAGCAG 
TTGCGTGGCA GTGAAGAGGA GTGCACTCTA ATGAAGAAAG AACTGGCAGC TCTGCAGCAC 
AGACTGGGGA ATAAGAGTCG ACAACTTAAG GAAGCGAGGA GGCAAGCGCA GGAGTGTGAG 
GAGGAGGGCA GGCGTGCCCT ACAGGAAGCC AGGCAACAAT TAGATGCTCA GCACTCGGCC 
TCTCAGGCAG AGCTGGAGAG ATTGCGGGAC CAAGAGGCCG TCAGCAGTTC CAGAGTCAAG 
AATTTGGAAG AGGAAGTGTT CAGAAGCAGA CAGGATTTGC TAGAGGCTCA GAGCCGCTCA 
ACTGCTTTGT CCCTTCAGCT ATCCTCAGTA GAACGAGAAA GAGCTGACAA CGAACAACAA 
CTCTGTCAAA TCAAGCGTGA GCTCCAGGAC CTGCAGTGTC TGTACAAGCA GAGCGTAGAA 
CATGCAGGAG AACAGGCCCA TCTTATTCAG CAGCTGGAGG GTCTTAACCT GGACACGCAA 
AGAGTCCTGC GCAGCCAGGA GGAGGCCCAC ACAGCGGACA CCGTCTCTTA CCAGAAGCTC 
TACAGCGAGC TGAGCGTGTC ATACCAGGCC CTTTGGCACA GCCAGGAACA GCTCCACCAG 
CGGGAAGCAG CCCTCAGCAT GCAGCTCAGC CAGAGAGATC AGCAGATCCA AGAACTCCAG 
GCACAGCTGC AGAGTTTCAC AGCAGCCGCG GGGCACTGTG AGGACTCTGG GTCTCCAGGT 
GGCAGCAGCA GTGACGGAGC TGACAGTCTG GAGAAAAGGA TTCAGCAACT GCAGGAATTA 
CTCGCTTTTA AGACTCAGGA GAATGAGGAT CTTCGGAAAG CCCATGCCAA ACATCATGAT 
CGTTTACGCG TTATTCAGAC CAACTACAAA ACAGTGACAG AGCGACTGAA AGAAGCTGAA 
GATACTCATG ACTTGGTGAA GCACAAAGTA CAATTTCCAC AGGACAGTGA GGGTTTATGG 
AAAGAACTGT TTTTCTATAA GGAGGAGAAC AGGAAGCTAC TCGCTGACAA AGCTAATATG 
GAGGAGGAAC TAAACGCAGC CCTGGACAGA ATTGCAGCAC AGGAGATCCA AGAGGATCGG 
CGGGAGGCAG CGCAAGCAGA GCTTCTGTTT CGGGAAGTGG CGGAGGAGGA GGTCACAAGG 
AGCAGCACCC CCATCAAGAC AACCATGCGA AGAGTGGAAG AGACTTTTAA GAAGATCGAG 
CAGCTACAAA GTCAAATGGT CTCTCTGGAG AGTGAGATTT TGCGGTTACG AGAAGACAAC 
CGGGCACTGG CCGGTGAACG GGGTGATCTG CAGGCCAGGG TTCAGATGCT GCAGCAAAGC 
AAGGAGGCAG AAGAGGCCCG GGCCAAAGAC GAACGCAACC GACTGATTTC CCAGATGCAG 
AGCTTTGAGA GAGAGGCAAA GGAGGCTGCG AAAGCTGCTG CCCAGGCCAG ACGCAGGCTG 
CTCAAGCTTC GACAGGAACT GGGCGTCCTC CGAGCAGAGA GAGATTTCCA TCGGTCCTCT 
GCTAGACGCA AGGTGAACAA CAAAGCCCCC AGGGTGGATG CCTCCAGCAG AACACACGTG 
CTTTCGACAG AGAACAGGAT GGACAGTCCC GCCAAGGACG ACTGGGAGGA CATGAGCGCA 
GACAGTGAGA GTGAAGATTT CTCTGACAGC CTGGAAAGTC GATATTCTGC TCGGACTCGT 
CAAGCTTCTC GAAAATCGAA GGGCTGCAGG TACATGCTGA TCTGCCCTAT AGACAGACCT 
AGAGAGGAAT CTCAAACCAG AAACATCCAA TTCCAAGGTG CCTCAATGCA GAAGATGAGG 
AGGAGAAAAA CATCAAAGCC CCATTCTGCT TTACGACAGC ATCTTCTGTC CCTCCAGCAG 
CAAGTGGCAG CACTACAGGC AGACAGACAA GCAGCACAGC AGCTTGCAAC TGAGCATAAC 
CACCGACAAG TCCAAACACA AGTGCAGATT GACTCTCTTC TCCAGCAACT CAATGCTAGC 
AAGCAGCTTT CAAAGAAGCA GGCATGCGAG TTGGCTGGTC TGGAGCAGCA GAAAGCATCT 
TTGCAGATGG AGTTAGAGCA GTGGAGACGT ATACGGCAGC AGACGTCACT GAACCCCCCC 
GCTTCCGAGC CGGAACGCCT GCAGGCCCAA GTCAAACAGC TCACTCAACG ACTCAAGAGT 
GCCAGCAGTG AGATGAGCAA ACACACTGCT GCTAACAAGA GCCTGAGGGC TCAGTTAGAT 
GAAAGAGATC AGACACTGAA GGAACTGCAC GAGAGGATTG GTGTTTTAGA GAGGGATGTA 
ACGATGAAAA GGCAGCTGGT GGAAGATCTC AGGAGCAGAC TAAAGATATG CCAGGACTCT 
GAGAGGAGCC ACAAAACAGC GACTGAAGAC TTGGAGAAGA AAATAAAATC ACTCTCTGAT 
GAGTCAACTA ACCGGAAAGC TCTTGTCGAC TCTCTCAAGA GAAGATTGAC AGTTGCCACT 
GCAGAAAAAA CTCAGCAGGA GACAAGCAGC CGCAAACTCA AAGAGGAGCT TCAGAAAAAG 
GAGCAGAGAG TGAGCGTCCT GCAGGCACGG ATAGGCGAGC GTGATCAGGC CATGACTGAG 
TTGGAACAGA CGGCGTCTCA ACAAATGCAT GCTTTGACCG AGCAGAGCTC TCAGACTCTG 
GAATCTCTGC AGAGAAAACT AACCCTCGCC GAGTCTCAGC TGCGCCAGTT TCACACATTC 
ATTCAGGTAT CTCAACTCTC CGACGTTTGC AATCAATTTA GCTTTAGGCC TTTTAAATAT 
CTATCTTATG TATTAAATTA CTGTTTAATC CTGTACATAT TACTGCTACC ACCTGGTTGT 
AATATGCATC TTAAATTTGT ATCCTATGTC CAAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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cntln cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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