dennd5b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dennd5b
Entrez Gene ID	100216203
Full Name	DENN/MADD domain containing 5B
General protein information	Preferred Names DENN/MADD domain containing 5B Names DENN domain-containing protein 5B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142172.1	NP_001135644.1	DENN domain-containing protein 5B
XM_031897714.1	XP_031753574.1	DENN domain-containing protein 5B isoform X1
XM_031897716.1	XP_031753576.1	DENN domain-containing protein 5B isoform X3
XM_031897715.1	XP_031753575.1	DENN domain-containing protein 5B isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DENND5B	XP_001137996.1
Bos taurus (cattle)	DENND5B	NP_001192417.1
Gallus gallus (chicken)	DENND5B	XP_004937958.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011351	XP_564133.3
Homo sapiens (human)	DENND5B	NP_659410.3
Macaca mulatta (Rhesus monkey)	DENND5B	XP_002798573.1
Mus musculus (house mouse)	Dennd5b	NP_796166.2
Rattus norvegicus (Norway rat)	Dennd5b	XP_006237774.1
Canis lupus familiaris (dog)	DENND5B	XP_005637062.1
Danio rerio (zebrafish)	dennd5b	NP_997809.1
Drosophila melanogaster (fruit fly)	CG7852	NP_647620.1
Xenopus tropicalis (tropical clawed frog)	dennd5b	NP_001135644.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dennd5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dennd5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa22624	NM_001142172.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa53361	XM_031897714.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa53362	XM_031897715.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa53363	XM_031897716.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dennd5b ( NM_001142172.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001135644.1 Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), mRNA.

Price & Availability↑

CloneID	OXa22624
Clone ID Related Accession (Same CDS sequence)	NM_001142172.1
Accession Version	NM_001142172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168530.1. ##Evidence-Data-START## Transcript exon combination :: BC168530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGGCC CCTCGGGCAC GGCCGGACCC CCCTGCCGCC TGGCTCATTA CTTCGTTATC 
TGCGGGACGG ACGCGGACAG TGGGCTCGAA CCGGATGAAC TGGCGGGGGA AAACTTTGAG 
CAGAGTCCAC TGAGGAGAAC CTTCAAGTCT AAAGTTCTGG CCCACTATCC GCAGAACGTC 
GAGTGGAGTC CATTCGATCA AGATGCTGTC AACATGCTGT GCATGCCCAA GGGCCTTTCG 
TTCCGGACAC AGGCCGACGA CAGGGACCCG GAGTTCCATT CGTTCATCAT CACGCGGGAG 
GACGGCTCGC GCACCTACGG CTTCGTGCTG ACCTTTTACG AGGAGGTGAC GAGCCGGCAG 
ATCTGCGCCG CCATGCAGAC GCTGTACCAG ATGCACAACG CGGAGCAGAG TAACAGCGTC 
TACGCCTCGT CGTCGTGCAG CATGGACTCG CTGGCGAGCA GCGCGGACGA GAGCGACGAC 
GCCTCATTGG CCAAGTCCCA GCGCTACAAC TCGTACGACA TCAGTAAGGA CACGCTGTAC 
GTCTCCAAGT GCATCTGTCT GATCACGCCG CTGCCGTTCA TGCAGTCGTG CAGGAAGTTC 
CTGACGCAGT TGTACAAGGC GGTCACCTCG CCGCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TACATCCATA ACATTCTGTA CGAGGTGCCG CTGCCCCCCC CGGGGAGGTC GCTCAAGTTC 
TACGGGGTAT ACGAGGCTAT TGTGTGCCAG AGGCCGGGGC CCAGCGAGCT GCCCCTCTCC 
GACTACCCCC TCAGGGAGAC CTTTGAGTTG TTGGGACTGG AAAACCTCGT GCAGCTTTTT 
ACCTGCGTCC TGCTGGAAAT GCAGATCCTG CTCTATTCCC AAGACTACCA GCGGCTAATG 
ACAGTGGCAG AGGGCATCAC CTCTCTCCTA TTCCCCTTTC AATGGCAGCA CGTCTACGTT 
CCCATCCTAC CCGCCTCCCT TCTGCATTTC CTCGATGCGC CTGTTCCGTA CCTGATGGGT 
CTTCAGTCCA AAGAAGGGAC CGACCGCTCC AAACTTGAAC TTCCTCAAGA GGCCAACCTG 
TGCTTTGTGG ATATCGATAA CCATTTCATC GAGCTGCCCG AGGAGTTCCC CCAGTTTCCC 
AACAAGCTGG AATTCATTCA GGAAATCTCG GAGGTCCTGC TGCAATACGG GATCCCCCCC 
GAGGGCAACT TGCACTGCAG CGAGAGCGCC ACCAAGCTGA AGAGCCTGGC CCTGGACGAC 
TGGGTCAACG ACAAGAAGAA CGGCAACCTC TCCGGCAACA ACATCAACAT GTACGAACTG 
CTAAAGGGCA ACGAGACCAT CGCCCGGCTC CAGGCGCTGA CTAAGCGGAC GGGGATAACC 
ATGGATAAAA TCAACCTGGC GGCGCCCCTA GTGGACGCGG AGGACGAGCA GAAAGTCCAA 
TGCGCCGAGA AGGAGCTTCA GGACTACAAA CTCAACGTGC AGCTCCGGGA GGTCTTCGCC 
AATCGCTTCA CACAGATGTT CTCCGACTAC GAGGCCTTCG TGATTCAGTC GGCCCACGAT 
ATGGAGTCGT GGCTCAACAA CAGGGAGCAG ATGCAGAACT TCGACAAGGC TTCCTTTCTG 
TCCGACCAGC CGGAGCCGTA CCTGCCGTTC CTGTCCCGTT TCATCGAGAC GCAGATGTTT 
GCCACCTTCA TAGACAATAA AATTATGTCC CTGTGGGACG AGAAGGAGCC GCTGCTGCGC 
GTGTTCGATT CCCGCATCCA GAAGTTGAGG CTGTACAACG GCCGCGCCCC CGCGCTCAGG 
TCCTCCAATT ATAAGAAATG CACCGCCCTG AAGGAGGCAG CCCAGGCCAT CGAGCAGCGG 
CTTACCAAGA TCGATCATAC GGCCATCCAC CCCCACCTGC TGGATATGAA GATCGGCCAG 
GGCAAGTACG AGCAGGGCTT CTTCCCCAAG CTGCAGTCCG ACGTCCTGAT GACCGGACCG 
ACCAATAACA ACCGATGGGT GAGCCGTAGT GCGACGGCGC AGCGGAGGAA AGACAAACTG 
CGGCAGCACT CTGAGCACCT GGGCCTAGAC AATGACTTAA GGGAGAAATA CATGCAGGAA 
GCCAGGAGTC TGGGCAAAAC TGTCAGGCAA CCAAAATTAT CTGATCTCTC CCCGGCGGTT 
ATTGCTCAGA CTAACTCCAA GTTTGTTGAA GGATTATTAA AAGAATGTCG TATGAAGACA 
AAGCGGATGT TGGTGGAAAA AATGGGCCAC GAAGCTGTGG AGTTGGGCCA CGGAGAGGCC 
AATATAACTG GGTTAGAAGA GAACACACTG ATTGCCAGCC TGTGTGACCT GTTAGAGAGA 
ATATGGAGTC ACGGCCTGCT TGTTAAACAG GGCAAGTCGG CGCTGTGGTC GCACCTGGTT 
CAGTTCCAGG AGAGAGAGGA GCGATTGGAG CGCACAGCGG AGTCGCCAAC GGCCCCAGGG 
CCAGACAGGA GGAAGTCAGA TACCGGCGTC AGTTTGCCGT CTCTACGAGT GTCGCTCATC 
CAAGACATGA GGCATATCCA GAACCTGAGT GAGATCAAGA CGGACGTGGG CCGAGCTCGC 
GGGTGGATAC GGTTGTCCCT GGAGAAGAAG CTCCTGTCTC AGCACCTCAA GCAGCTTCTG 
ACCAATCAGT CCCTCACCAG GAAGCTGTAC AAGCGCTACG CATTTCTCCG CTGCGAGGAG 
AAGAAGGAGC AGTTTCTCTA CCATCTCCTG TCTCTCAATG CCGTGGATTA CTTCTGCTTC 
ACCAGCGTCT TTACCACTAT CATGGTTCCT TACAGGGTTG TGATCATCCC CATCAAAAAA 
CTGAGCAACA CCATCACCAC GTCCAACCCG TGGATTTGTG TGTCGGGGGA GCTGGGGGAC 
ACCGGGGTCA TGCAGATTCC CAAAAACCTG CTGGAGATGA CCTTTGAGAG CCAGAATTTG 
GGCAAAATGA CTACAGTTCA GCTGGGACAC GACAACTCCG GCCTTTTGGC CAAGTGGCTC 
GTCGACTGCG TCGTGGTCCG AAGTGAGATC ACGGGACACA CGTACAAGTT CCCGTGTGGC 
CGCTGGTTGG GGAAAGGGGT AGATGACGGG AGCCTAGAAA GAGTTTTGAT TGGTGAACTG 
ATAACCCCCA CGTCGGATGA TGATCTTGGG AAACAAAGCC GCACCCCTCC CCAGCAGAAA 
TCTCCCACTG TGGCGCGCAG ACTAAGCATA ACCTCCCTTA CAGGAAAAAA TATCAAACCA 
AATGCTGGGC AGATCCAGGA AGGTATCGGA GAAGCCATAA ACAACATAGT GAAACATTTC 
CACAAACCTG AGAAAAAGAG AGGCAGCCTG ACCGTCCTGC TGTGCGGAGA GAGTGGCCTC 
GTCTCTGCTC TGGAGCAGCT CTTTCACCAC GGATTCAAAT CCGCTCGGAT ATTCCAGAAA 
AGCGTCTTCA TCTGGGACTT CATAGAGAAG GCCGTGGCTT ACTTTGAAAC TACCGACCAA 
ATAGGGGACG GTGAAGCGGA TCATCTGATC CCGAGGTCGT CCTGCAAGAC CTTCTGCCAC 
TACGTGAATG CGATCGTGGC AGCCCCCCGG CACATTGGGA AAGATGGGAA ATTCCAGCTC 
TTGGTCTGCC TAGGGGCAAG GGATCACTTA CTGGCCCTCT GGATCCCCCT GCTGGCTGAG 
TGCCCGGCCA TCACCAGGAT GTACGAGGAG AACGCCCTTC TCCGAGACCC CATGACGGTC 
AACTCGCTCA TACGGGTGCT GCAGACCTTA CAGGATTTCA ACATCGTGCT GGAGGGCTCC 
CTTGTTAAAG GCGTCGACGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135644.1
CDS	94..3897
Translation	MSGPSGTAGPPCRLAHYFVICGTDADSGLEPDELAGENFEQSPLRRTFKSKVLAHYPQNVEWSPFDQDAVNMLCMPKGLSFRTQADDRDPEFHSFIITREDGSRTYGFVLTFYEEVTSRQICAAMQTLYQMHNAEQSNSVYASSSCSMDSLASSADESDDASLAKSQRYNSYDISKDTLYVSKCICLITPLPFMQSCRKFLTQLYKAVTSPQPPPLPLESYIHNILYEVPLPPPGRSLKFYGVYEAIVCQRPGPSELPLSDYPLRETFELLGLENLVQLFTCVLLEMQILLYSQDYQRLMTVAEGITSLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHFIELPEEFPQFPNKLEFIQEISEVLLQYGIPPEGNLHCSESATKLKSLALDDWVNDKKNGNLSGNNINMYELLKGNETIARLQALTKRTGITMDKINLAAPLVDAEDEQKVQCAEKELQDYKLNVQLREVFANRFTQMFSDYEAFVIQSAHDMESWLNNREQMQNFDKASFLSDQPEPYLPFLSRFIETQMFATFIDNKIMSLWDEKEPLLRVFDSRIQKLRLYNGRAPALRSSNYKKCTALKEAAQAIEQRLTKIDHTAIHPHLLDMKIGQGKYEQGFFPKLQSDVLMTGPTNNNRWVSRSATAQRRKDKLRQHSEHLGLDNDLREKYMQEARSLGKTVRQPKLSDLSPAVIAQTNSKFVEGLLKECRMKTKRMLVEKMGHEAVELGHGEANITGLEENTLIASLCDLLERIWSHGLLVKQGKSALWSHLVQFQEREERLERTAESPTAPGPDRRKSDTGVSLPSLRVSLIQDMRHIQNLSEIKTDVGRARGWIRLSLEKKLLSQHLKQLLTNQSLTRKLYKRYAFLRCEEKKEQFLYHLLSLNAVDYFCFTSVFTTIMVPYRVVIIPIKKLSNTITTSNPWICVSGELGDTGVMQIPKNLLEMTFESQNLGKMTTVQLGHDNSGLLAKWLVDCVVVRSEITGHTYKFPCGRWLGKGVDDGSLERVLIGELITPTSDDDLGKQSRTPPQQKSPTVARRLSITSLTGKNIKPNAGQIQEGIGEAINNIVKHFHKPEKKRGSLTVLLCGESGLVSALEQLFHHGFKSARIFQKSVFIWDFIEKAVAYFETTDQIGDGEADHLIPRSSCKTFCHYVNAIVAAPRHIGKDGKFQLLVCLGARDHLLALWIPLLAECPAITRMYEENALLRDPMTVNSLIRVLQTLQDFNIVLEGSLVKGVDV

Target ORF information:

RefSeq Version	NM_001142172.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142172.1

ORF Insert Sequence:

ATGAGCGGCC CCTCGGGCAC GGCCGGACCC CCCTGCCGCC TGGCTCATTA CTTCGTTATC 
TGCGGGACGG ACGCGGACAG TGGGCTCGAA CCGGATGAAC TGGCGGGGGA AAACTTTGAG 
CAGAGTCCAC TGAGGAGAAC CTTCAAGTCT AAAGTTCTGG CCCACTATCC GCAGAACGTC 
GAGTGGAGTC CATTCGATCA AGATGCTGTC AACATGCTGT GCATGCCCAA GGGCCTTTCG 
TTCCGGACAC AGGCCGACGA CAGGGACCCG GAGTTCCATT CGTTCATCAT CACGCGGGAG 
GACGGCTCGC GCACCTACGG CTTCGTGCTG ACCTTTTACG AGGAGGTGAC GAGCCGGCAG 
ATCTGCGCCG CCATGCAGAC GCTGTACCAG ATGCACAACG CGGAGCAGAG TAACAGCGTC 
TACGCCTCGT CGTCGTGCAG CATGGACTCG CTGGCGAGCA GCGCGGACGA GAGCGACGAC 
GCCTCATTGG CCAAGTCCCA GCGCTACAAC TCGTACGACA TCAGTAAGGA CACGCTGTAC 
GTCTCCAAGT GCATCTGTCT GATCACGCCG CTGCCGTTCA TGCAGTCGTG CAGGAAGTTC 
CTGACGCAGT TGTACAAGGC GGTCACCTCG CCGCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TACATCCATA ACATTCTGTA CGAGGTGCCG CTGCCCCCCC CGGGGAGGTC GCTCAAGTTC 
TACGGGGTAT ACGAGGCTAT TGTGTGCCAG AGGCCGGGGC CCAGCGAGCT GCCCCTCTCC 
GACTACCCCC TCAGGGAGAC CTTTGAGTTG TTGGGACTGG AAAACCTCGT GCAGCTTTTT 
ACCTGCGTCC TGCTGGAAAT GCAGATCCTG CTCTATTCCC AAGACTACCA GCGGCTAATG 
ACAGTGGCAG AGGGCATCAC CTCTCTCCTA TTCCCCTTTC AATGGCAGCA CGTCTACGTT 
CCCATCCTAC CCGCCTCCCT TCTGCATTTC CTCGATGCGC CTGTTCCGTA CCTGATGGGT 
CTTCAGTCCA AAGAAGGGAC CGACCGCTCC AAACTTGAAC TTCCTCAAGA GGCCAACCTG 
TGCTTTGTGG ATATCGATAA CCATTTCATC GAGCTGCCCG AGGAGTTCCC CCAGTTTCCC 
AACAAGCTGG AATTCATTCA GGAAATCTCG GAGGTCCTGC TGCAATACGG GATCCCCCCC 
GAGGGCAACT TGCACTGCAG CGAGAGCGCC ACCAAGCTGA AGAGCCTGGC CCTGGACGAC 
TGGGTCAACG ACAAGAAGAA CGGCAACCTC TCCGGCAACA ACATCAACAT GTACGAACTG 
CTAAAGGGCA ACGAGACCAT CGCCCGGCTC CAGGCGCTGA CTAAGCGGAC GGGGATAACC 
ATGGATAAAA TCAACCTGGC GGCGCCCCTA GTGGACGCGG AGGACGAGCA GAAAGTCCAA 
TGCGCCGAGA AGGAGCTTCA GGACTACAAA CTCAACGTGC AGCTCCGGGA GGTCTTCGCC 
AATCGCTTCA CACAGATGTT CTCCGACTAC GAGGCCTTCG TGATTCAGTC GGCCCACGAT 
ATGGAGTCGT GGCTCAACAA CAGGGAGCAG ATGCAGAACT TCGACAAGGC TTCCTTTCTG 
TCCGACCAGC CGGAGCCGTA CCTGCCGTTC CTGTCCCGTT TCATCGAGAC GCAGATGTTT 
GCCACCTTCA TAGACAATAA AATTATGTCC CTGTGGGACG AGAAGGAGCC GCTGCTGCGC 
GTGTTCGATT CCCGCATCCA GAAGTTGAGG CTGTACAACG GCCGCGCCCC CGCGCTCAGG 
TCCTCCAATT ATAAGAAATG CACCGCCCTG AAGGAGGCAG CCCAGGCCAT CGAGCAGCGG 
CTTACCAAGA TCGATCATAC GGCCATCCAC CCCCACCTGC TGGATATGAA GATCGGCCAG 
GGCAAGTACG AGCAGGGCTT CTTCCCCAAG CTGCAGTCCG ACGTCCTGAT GACCGGACCG 
ACCAATAACA ACCGATGGGT GAGCCGTAGT GCGACGGCGC AGCGGAGGAA AGACAAACTG 
CGGCAGCACT CTGAGCACCT GGGCCTAGAC AATGACTTAA GGGAGAAATA CATGCAGGAA 
GCCAGGAGTC TGGGCAAAAC TGTCAGGCAA CCAAAATTAT CTGATCTCTC CCCGGCGGTT 
ATTGCTCAGA CTAACTCCAA GTTTGTTGAA GGATTATTAA AAGAATGTCG TATGAAGACA 
AAGCGGATGT TGGTGGAAAA AATGGGCCAC GAAGCTGTGG AGTTGGGCCA CGGAGAGGCC 
AATATAACTG GGTTAGAAGA GAACACACTG ATTGCCAGCC TGTGTGACCT GTTAGAGAGA 
ATATGGAGTC ACGGCCTGCT TGTTAAACAG GGCAAGTCGG CGCTGTGGTC GCACCTGGTT 
CAGTTCCAGG AGAGAGAGGA GCGATTGGAG CGCACAGCGG AGTCGCCAAC GGCCCCAGGG 
CCAGACAGGA GGAAGTCAGA TACCGGCGTC AGTTTGCCGT CTCTACGAGT GTCGCTCATC 
CAAGACATGA GGCATATCCA GAACCTGAGT GAGATCAAGA CGGACGTGGG CCGAGCTCGC 
GGGTGGATAC GGTTGTCCCT GGAGAAGAAG CTCCTGTCTC AGCACCTCAA GCAGCTTCTG 
ACCAATCAGT CCCTCACCAG GAAGCTGTAC AAGCGCTACG CATTTCTCCG CTGCGAGGAG 
AAGAAGGAGC AGTTTCTCTA CCATCTCCTG TCTCTCAATG CCGTGGATTA CTTCTGCTTC 
ACCAGCGTCT TTACCACTAT CATGGTTCCT TACAGGGTTG TGATCATCCC CATCAAAAAA 
CTGAGCAACA CCATCACCAC GTCCAACCCG TGGATTTGTG TGTCGGGGGA GCTGGGGGAC 
ACCGGGGTCA TGCAGATTCC CAAAAACCTG CTGGAGATGA CCTTTGAGAG CCAGAATTTG 
GGCAAAATGA CTACAGTTCA GCTGGGACAC GACAACTCCG GCCTTTTGGC CAAGTGGCTC 
GTCGACTGCG TCGTGGTCCG AAGTGAGATC ACGGGACACA CGTACAAGTT CCCGTGTGGC 
CGCTGGTTGG GGAAAGGGGT AGATGACGGG AGCCTAGAAA GAGTTTTGAT TGGTGAACTG 
ATAACCCCCA CGTCGGATGA TGATCTTGGG AAACAAAGCC GCACCCCTCC CCAGCAGAAA 
TCTCCCACTG TGGCGCGCAG ACTAAGCATA ACCTCCCTTA CAGGAAAAAA TATCAAACCA 
AATGCTGGGC AGATCCAGGA AGGTATCGGA GAAGCCATAA ACAACATAGT GAAACATTTC 
CACAAACCTG AGAAAAAGAG AGGCAGCCTG ACCGTCCTGC TGTGCGGAGA GAGTGGCCTC 
GTCTCTGCTC TGGAGCAGCT CTTTCACCAC GGATTCAAAT CCGCTCGGAT ATTCCAGAAA 
AGCGTCTTCA TCTGGGACTT CATAGAGAAG GCCGTGGCTT ACTTTGAAAC TACCGACCAA 
ATAGGGGACG GTGAAGCGGA TCATCTGATC CCGAGGTCGT CCTGCAAGAC CTTCTGCCAC 
TACGTGAATG CGATCGTGGC AGCCCCCCGG CACATTGGGA AAGATGGGAA ATTCCAGCTC 
TTGGTCTGCC TAGGGGCAAG GGATCACTTA CTGGCCCTCT GGATCCCCCT GCTGGCTGAG 
TGCCCGGCCA TCACCAGGAT GTACGAGGAG AACGCCCTTC TCCGAGACCC CATGACGGTC 
AACTCGCTCA TACGGGTGCT GCAGACCTTA CAGGATTTCA ACATCGTGCT GGAGGGCTCC 
CTTGTTAAAG GCGTCGACGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5b ( XM_031897714.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753574.1 Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa53361
Clone ID Related Accession (Same CDS sequence)	XM_031897714.1
Accession Version	XM_031897714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCC CCTCGGGCAC GGCCGGACCC CCCTGCCGCC TGGCTCATTA CTTCGTTATC 
TGCGGGACGG ACGCGGACAG TGGGCTCGAA CCGGATGAAC TGGCGGTTTT GTACCAGTGG 
CTCGAAGCAG ACCGGCATGG CAAGGGCCAC GACACAGGGG AAAACTTTGA GCAGAGTCCA 
CTGAGGAGAA CCTTCAAGTC TAAAGTTCTG GCCCACTATC CGCAGAACGT CGAGTGGAGT 
CCATTCGATC AAGATGCTGT CAACATGCTG TGCATGCCCA AGGGCCTATC GTTCCGGACA 
CAGGCCGACG ACAGGGACCC GGAGTTCCAT TCGTTCATCA TCACGCGGGA GGACGGCTCG 
CGCACCTACG GCTTCGTGCT GACCTTTTAC GAGGAGGTGA CGAGCCGGCA GATCTGCGCC 
GCCATGCAGA CGCTGTACCA GATGCACAAC GCGGAGCAGA GTAACAGCGT CTACGCCTCG 
TCGTCGTGCA GCATGGACTC GCTGGCGAGC AGCGCGGACG AGAGCGACGA CGCCTCATTG 
GCCAAGTCCC AGCGCTACAA CTCGTACGAC ATCAGTAAGG ACACGCTGTA CGTCTCCAAG 
TGCATCTGTC TGATCACGCC GCTGCCGTTC ATGCAGTCGT GCAGGAAGTT CCTGACGCAG 
TTGTACAAGG CGGTCACCTC GCCGCAGCCC CCGCCGCTGC CGCTGGAGAG CTACATCCAT 
AACATTCTGT ACGAGGTGCC GCTGCCCCCC CCGGGGAGGT CGCTCAAGTT CTACGGGGTA 
TACGAGGCTA TTGTGTGCCA GAGGCCGGGG CCCAGCGAGC TGCCCCTCTC CGACTACCCC 
CTCAGGGAGA CCTTTGAGTT GTTGGGACTG GAAAACCTCG TGCAGCTTTT TACCTGCGTC 
CTGCTGGAAA TGCAGATCCT GCTCTATTCC CAAGACTACC AGCGGCTAAT GACAGTGGCA 
GAGGGCATCA CCTCTCTCCT ATTCCCCTTT CAATGGCAGC ACGTCTACGT TCCCATCCTA 
CCCGCCTCCC TTCTGCATTT CCTCGATGCG CCTGTTCCGT ACCTGATGGG TCTTCAGTCC 
AAAGAAGGGA CCGACCGCTC CAAACTTGAA CTTCCTCAAG AGGCCAACCT GTGCTTTGTG 
GATATCGATA ACCATTTCAT CGAGCTGCCC GAGGAGTTCC CCCAGTTTCC CAACAAGCTG 
GAATTCATTC AGGAAATCTC GGAGGTCCTG CTGCAATACG GGATCCCCCC CGAGGGCAAC 
TTGCACTGCA GCGAGAGCGC CACCAAGCTG AAGAGCCTGG CCCTGGACGA CTGGGTCAAC 
GACAAGAAGA ACGGCAACCT CTCCGGCAAC AACATCAACA TGTACGAACT GCTAAAGGGC 
AACGAGACCA TCGCCCGGCT CCAGGCGCTG ACTAAGCGGA CGGGGATAAC CATGGATAAA 
ATCAACCTGG CGGCGCCCCT AGTGGACGCG GAGGACGAGC AGAAAGTCCA ATGCGCCGAG 
AAGGAGCTTC AGGACTACAA ACTCAACGTG CAGCTCCGGG AGGTCTTCGC CAATCGCTTC 
ACACAGATGT TCTCCGACTA CGAGGCCTTC GTGATTCAGT CGGCCCACGA TATGGAGTCG 
TGGCTCAACA ACAGGGAGCA GATGCAGAAC TTCGACAAGG CTTCCTTTCT GTCCGACCAG 
CCGGAGCCGT ACCTGCCGTT CCTGTCCCGT TTCATCGAGA CGCAGATGTT TGCCACCTTC 
ATAGACAATA AAATTATGTC CCTGTGGGAC GAGAAGGAGC CGCTGCTGCG CGTGTTCGAT 
TCCCGCATCC AGAAGTTGAG GCTGTACAAC GGCCGCGCCC CCGCGCTCAG GTCCTCCAAT 
TATAAGAAAT GCACCGCCCT GAAGGAGGCA GCCCAGGCCA TCGAGCAGCG GCTTACCAAG 
ATCGATCATA CGGCCATCCA CCCCCACCTG CTGGATATGA AGATCGGCCA GGGCAAGTAC 
GAGCAGGGCT TCTTCCCCAA GCTGCAGTCC GACGTCCTGA TGACCGGACC GACCAATAAC 
AACCGATGGG TGAGCCGTAG TGCGACGGCG CAGCGGAGGA AAGACAAACT GCGGCAGCAC 
TCTGAGCACC TGGGCCTAGA CAATGACTTA AGGGAGCCCT CTGAGGAGAT AGTGATCCGG 
CAGAACTCTA TGGCTTTCTG GGAGTGGGAT CAGGGGTCCC CCCCTCCAGC ACGGCCTCCA 
AAAAAATCCT ACTCTGAGTG CTGCCTGAAA TACATGCAGG AAGCCAGGAG TCTGGGCAAA 
ACTGTCAGGC AACCGAAATT ATCTGATCTC TCCCCGGCGG TTATTGCTCA GACTAACTCC 
AAGTTTGTTG AAGGATTATT AAAAGAATGT CGTATGAAGA CAAAGCGGAT GTTGGTGGAA 
AAAATGGGCC ACGAAGCTGT GGAGTTGGGC CACGGAGAGG CCAATATAAC TGGGTTAGAA 
GAGAACACAC TGATTGCCAG CCTGTGTGAC CTGTTAGAGA GAATATGGAG TCACGGCCTG 
CTTGTTAAAC AGGGCAAGTC GGCGCTGTGG TCGCACCTGG TTCAGTTCCA GGAGAGAGAG 
GAGCGATTGG AGCGCACAGC GGAGTCGCCA ACGGCCCCAG GGCCAGACAG GAGGAAGTCA 
GATACCGGCG TCAGTTTGCC GTCTCTACGA GTGTCGCTCA TCCAAGACAT GAGGCATATC 
CAGAACCTGA GTGAGATCAA GACGGACGTG GGCCGAGCTC GCGGGTGGAT ACGGTTGTCC 
CTGGAGAAGA AGCTCCTGTC TCAGCACCTC AAGCAGCTTC TGACCAATCA GTCCCTCACC 
AGGAAGCTGT ACAAGCGCTA CGCATTTCTC CGCTGCGAGG AGGAGAAGGA GCAGTTTCTC 
TACCATCTCC TGTCTCTCAA TGCCGTGGAT TACTTCTGCT TCACCAGCGT CTTTACCACT 
ATCATGGTTC CTTACAGGGT TGTGATCATC CCCATCAAAA AACTGAGCAA CGCCATCACC 
ACGTCCAACC CGTGGATTTG TGTGTCGGGG GAGCTGGGGG ACACCGGGGT CATGCAGATT 
CCCAAAAACC TGCTGGAGAT GACCTTTGAG AGCCAGAATT TGGGCAAAAT GACTACAGTT 
CAGCTGGGTC ACGACAACTC CGGCCTTTTG GCCAAGTGGC TCGTCGACTG CGTCGTGGTC 
CGAAGTGAGA TCACGGGACA CACGTACAAG TTCCCGTGTG GCCGCTGGCT GGGGAAAGGG 
GTAGATGACG GGAGCCTAGA AAGAGTTTTG ATTGGTGAAC TGATAACCCC CACGTCGGAT 
GATGATCTTG GGAAACAAAG CCGCACCCCT CCCCAGCAGA AATCTCCCAC CGTGGCGCGC 
AGACTAAGCA TAACCTCCCT TACAGGAAAA AATATCAAAC CAAATGCTGG GCAGATCCAG 
GAAGGTATCG GAGAAGCCAT AAACAACATA GTGAAACATT TCCACAAACC TGAGAAAGAG 
AGAGGCAGCC TGACCGTCCT GCTGTGCGGA GAGAGTGGCC TCGTCTCGGC TCTGGAGCAG 
CTCTTTCACC ACGGATTCAA ATCCGCTCGG ATATTCCAGA AAAGCGTCTT CATCTGGGAC 
TTCATAGAGA AGGCCGTGGC TTACTTTGAA ACTACCGACC AAATAGGGGA CGGTGAAGCG 
GATCATCTGA TCCCGAGGTC GTCCTGCAAG ACCTTCTGCC ACTACGTGAA TGCGATCGTG 
GCAGCCCCCC GGCACATTGG AAAAGATGGG AAATTCCAGC TCTTGGTCTG CCTAGGGGCA 
AGGGATCACT TACTGGCCCT CTGGATCCCC CTGCTGGCTG AGTGCCCGGC CATCACCAGG 
ATGTACGAGG AGAACGCCCT TCTCCGAGAC CCCATGACGG TCAACTCGCT CATACGGGTG 
CTGCAGACCT TACAGGATTT CAACATCGTG CTGGAGGGCT CCCTTGTTAA AGGCGTCGAC 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753574.1
CDS	192..4157
Translation	MSGPSGTAGPPCRLAHYFVICGTDADSGLEPDELAVLYQWLEADRHGKGHDTGENFEQSPLRRTFKSKVLAHYPQNVEWSPFDQDAVNMLCMPKGLSFRTQADDRDPEFHSFIITREDGSRTYGFVLTFYEEVTSRQICAAMQTLYQMHNAEQSNSVYASSSCSMDSLASSADESDDASLAKSQRYNSYDISKDTLYVSKCICLITPLPFMQSCRKFLTQLYKAVTSPQPPPLPLESYIHNILYEVPLPPPGRSLKFYGVYEAIVCQRPGPSELPLSDYPLRETFELLGLENLVQLFTCVLLEMQILLYSQDYQRLMTVAEGITSLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHFIELPEEFPQFPNKLEFIQEISEVLLQYGIPPEGNLHCSESATKLKSLALDDWVNDKKNGNLSGNNINMYELLKGNETIARLQALTKRTGITMDKINLAAPLVDAEDEQKVQCAEKELQDYKLNVQLREVFANRFTQMFSDYEAFVIQSAHDMESWLNNREQMQNFDKASFLSDQPEPYLPFLSRFIETQMFATFIDNKIMSLWDEKEPLLRVFDSRIQKLRLYNGRAPALRSSNYKKCTALKEAAQAIEQRLTKIDHTAIHPHLLDMKIGQGKYEQGFFPKLQSDVLMTGPTNNNRWVSRSATAQRRKDKLRQHSEHLGLDNDLREPSEEIVIRQNSMAFWEWDQGSPPPARPPKKSYSECCLKYMQEARSLGKTVRQPKLSDLSPAVIAQTNSKFVEGLLKECRMKTKRMLVEKMGHEAVELGHGEANITGLEENTLIASLCDLLERIWSHGLLVKQGKSALWSHLVQFQEREERLERTAESPTAPGPDRRKSDTGVSLPSLRVSLIQDMRHIQNLSEIKTDVGRARGWIRLSLEKKLLSQHLKQLLTNQSLTRKLYKRYAFLRCEEEKEQFLYHLLSLNAVDYFCFTSVFTTIMVPYRVVIIPIKKLSNAITTSNPWICVSGELGDTGVMQIPKNLLEMTFESQNLGKMTTVQLGHDNSGLLAKWLVDCVVVRSEITGHTYKFPCGRWLGKGVDDGSLERVLIGELITPTSDDDLGKQSRTPPQQKSPTVARRLSITSLTGKNIKPNAGQIQEGIGEAINNIVKHFHKPEKERGSLTVLLCGESGLVSALEQLFHHGFKSARIFQKSVFIWDFIEKAVAYFETTDQIGDGEADHLIPRSSCKTFCHYVNAIVAAPRHIGKDGKFQLLVCLGARDHLLALWIPLLAECPAITRMYEENALLRDPMTVNSLIRVLQTLQDFNIVLEGSLVKGVDV

Target ORF information:

RefSeq Version	XM_031897714.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897714.1

ORF Insert Sequence:

ATGAGCGGCC CCTCGGGCAC GGCCGGACCC CCCTGCCGCC TGGCTCATTA CTTCGTTATC 
TGCGGGACGG ACGCGGACAG TGGGCTCGAA CCGGATGAAC TGGCGGTTTT GTACCAGTGG 
CTCGAAGCAG ACCGGCATGG CAAGGGCCAC GACACAGGGG AAAACTTTGA GCAGAGTCCA 
CTGAGGAGAA CCTTCAAGTC TAAAGTTCTG GCCCACTATC CGCAGAACGT CGAGTGGAGT 
CCATTCGATC AAGATGCTGT CAACATGCTG TGCATGCCCA AGGGCCTATC GTTCCGGACA 
CAGGCCGACG ACAGGGACCC GGAGTTCCAT TCGTTCATCA TCACGCGGGA GGACGGCTCG 
CGCACCTACG GCTTCGTGCT GACCTTTTAC GAGGAGGTGA CGAGCCGGCA GATCTGCGCC 
GCCATGCAGA CGCTGTACCA GATGCACAAC GCGGAGCAGA GTAACAGCGT CTACGCCTCG 
TCGTCGTGCA GCATGGACTC GCTGGCGAGC AGCGCGGACG AGAGCGACGA CGCCTCATTG 
GCCAAGTCCC AGCGCTACAA CTCGTACGAC ATCAGTAAGG ACACGCTGTA CGTCTCCAAG 
TGCATCTGTC TGATCACGCC GCTGCCGTTC ATGCAGTCGT GCAGGAAGTT CCTGACGCAG 
TTGTACAAGG CGGTCACCTC GCCGCAGCCC CCGCCGCTGC CGCTGGAGAG CTACATCCAT 
AACATTCTGT ACGAGGTGCC GCTGCCCCCC CCGGGGAGGT CGCTCAAGTT CTACGGGGTA 
TACGAGGCTA TTGTGTGCCA GAGGCCGGGG CCCAGCGAGC TGCCCCTCTC CGACTACCCC 
CTCAGGGAGA CCTTTGAGTT GTTGGGACTG GAAAACCTCG TGCAGCTTTT TACCTGCGTC 
CTGCTGGAAA TGCAGATCCT GCTCTATTCC CAAGACTACC AGCGGCTAAT GACAGTGGCA 
GAGGGCATCA CCTCTCTCCT ATTCCCCTTT CAATGGCAGC ACGTCTACGT TCCCATCCTA 
CCCGCCTCCC TTCTGCATTT CCTCGATGCG CCTGTTCCGT ACCTGATGGG TCTTCAGTCC 
AAAGAAGGGA CCGACCGCTC CAAACTTGAA CTTCCTCAAG AGGCCAACCT GTGCTTTGTG 
GATATCGATA ACCATTTCAT CGAGCTGCCC GAGGAGTTCC CCCAGTTTCC CAACAAGCTG 
GAATTCATTC AGGAAATCTC GGAGGTCCTG CTGCAATACG GGATCCCCCC CGAGGGCAAC 
TTGCACTGCA GCGAGAGCGC CACCAAGCTG AAGAGCCTGG CCCTGGACGA CTGGGTCAAC 
GACAAGAAGA ACGGCAACCT CTCCGGCAAC AACATCAACA TGTACGAACT GCTAAAGGGC 
AACGAGACCA TCGCCCGGCT CCAGGCGCTG ACTAAGCGGA CGGGGATAAC CATGGATAAA 
ATCAACCTGG CGGCGCCCCT AGTGGACGCG GAGGACGAGC AGAAAGTCCA ATGCGCCGAG 
AAGGAGCTTC AGGACTACAA ACTCAACGTG CAGCTCCGGG AGGTCTTCGC CAATCGCTTC 
ACACAGATGT TCTCCGACTA CGAGGCCTTC GTGATTCAGT CGGCCCACGA TATGGAGTCG 
TGGCTCAACA ACAGGGAGCA GATGCAGAAC TTCGACAAGG CTTCCTTTCT GTCCGACCAG 
CCGGAGCCGT ACCTGCCGTT CCTGTCCCGT TTCATCGAGA CGCAGATGTT TGCCACCTTC 
ATAGACAATA AAATTATGTC CCTGTGGGAC GAGAAGGAGC CGCTGCTGCG CGTGTTCGAT 
TCCCGCATCC AGAAGTTGAG GCTGTACAAC GGCCGCGCCC CCGCGCTCAG GTCCTCCAAT 
TATAAGAAAT GCACCGCCCT GAAGGAGGCA GCCCAGGCCA TCGAGCAGCG GCTTACCAAG 
ATCGATCATA CGGCCATCCA CCCCCACCTG CTGGATATGA AGATCGGCCA GGGCAAGTAC 
GAGCAGGGCT TCTTCCCCAA GCTGCAGTCC GACGTCCTGA TGACCGGACC GACCAATAAC 
AACCGATGGG TGAGCCGTAG TGCGACGGCG CAGCGGAGGA AAGACAAACT GCGGCAGCAC 
TCTGAGCACC TGGGCCTAGA CAATGACTTA AGGGAGCCCT CTGAGGAGAT AGTGATCCGG 
CAGAACTCTA TGGCTTTCTG GGAGTGGGAT CAGGGGTCCC CCCCTCCAGC ACGGCCTCCA 
AAAAAATCCT ACTCTGAGTG CTGCCTGAAA TACATGCAGG AAGCCAGGAG TCTGGGCAAA 
ACTGTCAGGC AACCGAAATT ATCTGATCTC TCCCCGGCGG TTATTGCTCA GACTAACTCC 
AAGTTTGTTG AAGGATTATT AAAAGAATGT CGTATGAAGA CAAAGCGGAT GTTGGTGGAA 
AAAATGGGCC ACGAAGCTGT GGAGTTGGGC CACGGAGAGG CCAATATAAC TGGGTTAGAA 
GAGAACACAC TGATTGCCAG CCTGTGTGAC CTGTTAGAGA GAATATGGAG TCACGGCCTG 
CTTGTTAAAC AGGGCAAGTC GGCGCTGTGG TCGCACCTGG TTCAGTTCCA GGAGAGAGAG 
GAGCGATTGG AGCGCACAGC GGAGTCGCCA ACGGCCCCAG GGCCAGACAG GAGGAAGTCA 
GATACCGGCG TCAGTTTGCC GTCTCTACGA GTGTCGCTCA TCCAAGACAT GAGGCATATC 
CAGAACCTGA GTGAGATCAA GACGGACGTG GGCCGAGCTC GCGGGTGGAT ACGGTTGTCC 
CTGGAGAAGA AGCTCCTGTC TCAGCACCTC AAGCAGCTTC TGACCAATCA GTCCCTCACC 
AGGAAGCTGT ACAAGCGCTA CGCATTTCTC CGCTGCGAGG AGGAGAAGGA GCAGTTTCTC 
TACCATCTCC TGTCTCTCAA TGCCGTGGAT TACTTCTGCT TCACCAGCGT CTTTACCACT 
ATCATGGTTC CTTACAGGGT TGTGATCATC CCCATCAAAA AACTGAGCAA CGCCATCACC 
ACGTCCAACC CGTGGATTTG TGTGTCGGGG GAGCTGGGGG ACACCGGGGT CATGCAGATT 
CCCAAAAACC TGCTGGAGAT GACCTTTGAG AGCCAGAATT TGGGCAAAAT GACTACAGTT 
CAGCTGGGTC ACGACAACTC CGGCCTTTTG GCCAAGTGGC TCGTCGACTG CGTCGTGGTC 
CGAAGTGAGA TCACGGGACA CACGTACAAG TTCCCGTGTG GCCGCTGGCT GGGGAAAGGG 
GTAGATGACG GGAGCCTAGA AAGAGTTTTG ATTGGTGAAC TGATAACCCC CACGTCGGAT 
GATGATCTTG GGAAACAAAG CCGCACCCCT CCCCAGCAGA AATCTCCCAC CGTGGCGCGC 
AGACTAAGCA TAACCTCCCT TACAGGAAAA AATATCAAAC CAAATGCTGG GCAGATCCAG 
GAAGGTATCG GAGAAGCCAT AAACAACATA GTGAAACATT TCCACAAACC TGAGAAAGAG 
AGAGGCAGCC TGACCGTCCT GCTGTGCGGA GAGAGTGGCC TCGTCTCGGC TCTGGAGCAG 
CTCTTTCACC ACGGATTCAA ATCCGCTCGG ATATTCCAGA AAAGCGTCTT CATCTGGGAC 
TTCATAGAGA AGGCCGTGGC TTACTTTGAA ACTACCGACC AAATAGGGGA CGGTGAAGCG 
GATCATCTGA TCCCGAGGTC GTCCTGCAAG ACCTTCTGCC ACTACGTGAA TGCGATCGTG 
GCAGCCCCCC GGCACATTGG AAAAGATGGG AAATTCCAGC TCTTGGTCTG CCTAGGGGCA 
AGGGATCACT TACTGGCCCT CTGGATCCCC CTGCTGGCTG AGTGCCCGGC CATCACCAGG 
ATGTACGAGG AGAACGCCCT TCTCCGAGAC CCCATGACGG TCAACTCGCT CATACGGGTG 
CTGCAGACCT TACAGGATTT CAACATCGTG CTGGAGGGCT CCCTTGTTAA AGGCGTCGAC 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5b ( XM_031897715.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753575.1 Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), transcript variant X2, mRNA.

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CloneID	OXa53362
Clone ID Related Accession (Same CDS sequence)	XM_031897715.1
Accession Version	XM_031897715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCC CCTCGGGCAC GGCCGGACCC CCCTGCCGCC TGGCTCATTA CTTCGTTATC 
TGCGGGACGG ACGCGGACAG TGGGCTCGAA CCGGATGAAC TGGCGGGGGA AAACTTTGAG 
CAGAGTCCAC TGAGGAGAAC CTTCAAGTCT AAAGTTCTGG CCCACTATCC GCAGAACGTC 
GAGTGGAGTC CATTCGATCA AGATGCTGTC AACATGCTGT GCATGCCCAA GGGCCTATCG 
TTCCGGACAC AGGCCGACGA CAGGGACCCG GAGTTCCATT CGTTCATCAT CACGCGGGAG 
GACGGCTCGC GCACCTACGG CTTCGTGCTG ACCTTTTACG AGGAGGTGAC GAGCCGGCAG 
ATCTGCGCCG CCATGCAGAC GCTGTACCAG ATGCACAACG CGGAGCAGAG TAACAGCGTC 
TACGCCTCGT CGTCGTGCAG CATGGACTCG CTGGCGAGCA GCGCGGACGA GAGCGACGAC 
GCCTCATTGG CCAAGTCCCA GCGCTACAAC TCGTACGACA TCAGTAAGGA CACGCTGTAC 
GTCTCCAAGT GCATCTGTCT GATCACGCCG CTGCCGTTCA TGCAGTCGTG CAGGAAGTTC 
CTGACGCAGT TGTACAAGGC GGTCACCTCG CCGCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TACATCCATA ACATTCTGTA CGAGGTGCCG CTGCCCCCCC CGGGGAGGTC GCTCAAGTTC 
TACGGGGTAT ACGAGGCTAT TGTGTGCCAG AGGCCGGGGC CCAGCGAGCT GCCCCTCTCC 
GACTACCCCC TCAGGGAGAC CTTTGAGTTG TTGGGACTGG AAAACCTCGT GCAGCTTTTT 
ACCTGCGTCC TGCTGGAAAT GCAGATCCTG CTCTATTCCC AAGACTACCA GCGGCTAATG 
ACAGTGGCAG AGGGCATCAC CTCTCTCCTA TTCCCCTTTC AATGGCAGCA CGTCTACGTT 
CCCATCCTAC CCGCCTCCCT TCTGCATTTC CTCGATGCGC CTGTTCCGTA CCTGATGGGT 
CTTCAGTCCA AAGAAGGGAC CGACCGCTCC AAACTTGAAC TTCCTCAAGA GGCCAACCTG 
TGCTTTGTGG ATATCGATAA CCATTTCATC GAGCTGCCCG AGGAGTTCCC CCAGTTTCCC 
AACAAGCTGG AATTCATTCA GGAAATCTCG GAGGTCCTGC TGCAATACGG GATCCCCCCC 
GAGGGCAACT TGCACTGCAG CGAGAGCGCC ACCAAGCTGA AGAGCCTGGC CCTGGACGAC 
TGGGTCAACG ACAAGAAGAA CGGCAACCTC TCCGGCAACA ACATCAACAT GTACGAACTG 
CTAAAGGGCA ACGAGACCAT CGCCCGGCTC CAGGCGCTGA CTAAGCGGAC GGGGATAACC 
ATGGATAAAA TCAACCTGGC GGCGCCCCTA GTGGACGCGG AGGACGAGCA GAAAGTCCAA 
TGCGCCGAGA AGGAGCTTCA GGACTACAAA CTCAACGTGC AGCTCCGGGA GGTCTTCGCC 
AATCGCTTCA CACAGATGTT CTCCGACTAC GAGGCCTTCG TGATTCAGTC GGCCCACGAT 
ATGGAGTCGT GGCTCAACAA CAGGGAGCAG ATGCAGAACT TCGACAAGGC TTCCTTTCTG 
TCCGACCAGC CGGAGCCGTA CCTGCCGTTC CTGTCCCGTT TCATCGAGAC GCAGATGTTT 
GCCACCTTCA TAGACAATAA AATTATGTCC CTGTGGGACG AGAAGGAGCC GCTGCTGCGC 
GTGTTCGATT CCCGCATCCA GAAGTTGAGG CTGTACAACG GCCGCGCCCC CGCGCTCAGG 
TCCTCCAATT ATAAGAAATG CACCGCCCTG AAGGAGGCAG CCCAGGCCAT CGAGCAGCGG 
CTTACCAAGA TCGATCATAC GGCCATCCAC CCCCACCTGC TGGATATGAA GATCGGCCAG 
GGCAAGTACG AGCAGGGCTT CTTCCCCAAG CTGCAGTCCG ACGTCCTGAT GACCGGACCG 
ACCAATAACA ACCGATGGGT GAGCCGTAGT GCGACGGCGC AGCGGAGGAA AGACAAACTG 
CGGCAGCACT CTGAGCACCT GGGCCTAGAC AATGACTTAA GGGAGCCCTC TGAGGAGATA 
GTGATCCGGC AGAACTCTAT GGCTTTCTGG GAGTGGGATC AGGGGTCCCC CCCTCCAGCA 
CGGCCTCCAA AAAAATCCTA CTCTGAGTGC TGCCTGAAAT ACATGCAGGA AGCCAGGAGT 
CTGGGCAAAA CTGTCAGGCA ACCGAAATTA TCTGATCTCT CCCCGGCGGT TATTGCTCAG 
ACTAACTCCA AGTTTGTTGA AGGATTATTA AAAGAATGTC GTATGAAGAC AAAGCGGATG 
TTGGTGGAAA AAATGGGCCA CGAAGCTGTG GAGTTGGGCC ACGGAGAGGC CAATATAACT 
GGGTTAGAAG AGAACACACT GATTGCCAGC CTGTGTGACC TGTTAGAGAG AATATGGAGT 
CACGGCCTGC TTGTTAAACA GGGCAAGTCG GCGCTGTGGT CGCACCTGGT TCAGTTCCAG 
GAGAGAGAGG AGCGATTGGA GCGCACAGCG GAGTCGCCAA CGGCCCCAGG GCCAGACAGG 
AGGAAGTCAG ATACCGGCGT CAGTTTGCCG TCTCTACGAG TGTCGCTCAT CCAAGACATG 
AGGCATATCC AGAACCTGAG TGAGATCAAG ACGGACGTGG GCCGAGCTCG CGGGTGGATA 
CGGTTGTCCC TGGAGAAGAA GCTCCTGTCT CAGCACCTCA AGCAGCTTCT GACCAATCAG 
TCCCTCACCA GGAAGCTGTA CAAGCGCTAC GCATTTCTCC GCTGCGAGGA GGAGAAGGAG 
CAGTTTCTCT ACCATCTCCT GTCTCTCAAT GCCGTGGATT ACTTCTGCTT CACCAGCGTC 
TTTACCACTA TCATGGTTCC TTACAGGGTT GTGATCATCC CCATCAAAAA ACTGAGCAAC 
GCCATCACCA CGTCCAACCC GTGGATTTGT GTGTCGGGGG AGCTGGGGGA CACCGGGGTC 
ATGCAGATTC CCAAAAACCT GCTGGAGATG ACCTTTGAGA GCCAGAATTT GGGCAAAATG 
ACTACAGTTC AGCTGGGTCA CGACAACTCC GGCCTTTTGG CCAAGTGGCT CGTCGACTGC 
GTCGTGGTCC GAAGTGAGAT CACGGGACAC ACGTACAAGT TCCCGTGTGG CCGCTGGCTG 
GGGAAAGGGG TAGATGACGG GAGCCTAGAA AGAGTTTTGA TTGGTGAACT GATAACCCCC 
ACGTCGGATG ATGATCTTGG GAAACAAAGC CGCACCCCTC CCCAGCAGAA ATCTCCCACC 
GTGGCGCGCA GACTAAGCAT AACCTCCCTT ACAGGAAAAA ATATCAAACC AAATGCTGGG 
CAGATCCAGG AAGGTATCGG AGAAGCCATA AACAACATAG TGAAACATTT CCACAAACCT 
GAGAAAGAGA GAGGCAGCCT GACCGTCCTG CTGTGCGGAG AGAGTGGCCT CGTCTCGGCT 
CTGGAGCAGC TCTTTCACCA CGGATTCAAA TCCGCTCGGA TATTCCAGAA AAGCGTCTTC 
ATCTGGGACT TCATAGAGAA GGCCGTGGCT TACTTTGAAA CTACCGACCA AATAGGGGAC 
GGTGAAGCGG ATCATCTGAT CCCGAGGTCG TCCTGCAAGA CCTTCTGCCA CTACGTGAAT 
GCGATCGTGG CAGCCCCCCG GCACATTGGA AAAGATGGGA AATTCCAGCT CTTGGTCTGC 
CTAGGGGCAA GGGATCACTT ACTGGCCCTC TGGATCCCCC TGCTGGCTGA GTGCCCGGCC 
ATCACCAGGA TGTACGAGGA GAACGCCCTT CTCCGAGACC CCATGACGGT CAACTCGCTC 
ATACGGGTGC TGCAGACCTT ACAGGATTTC AACATCGTGC TGGAGGGCTC CCTTGTTAAA 
GGCGTCGACG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753575.1
CDS	192..4106
Translation	MSGPSGTAGPPCRLAHYFVICGTDADSGLEPDELAGENFEQSPLRRTFKSKVLAHYPQNVEWSPFDQDAVNMLCMPKGLSFRTQADDRDPEFHSFIITREDGSRTYGFVLTFYEEVTSRQICAAMQTLYQMHNAEQSNSVYASSSCSMDSLASSADESDDASLAKSQRYNSYDISKDTLYVSKCICLITPLPFMQSCRKFLTQLYKAVTSPQPPPLPLESYIHNILYEVPLPPPGRSLKFYGVYEAIVCQRPGPSELPLSDYPLRETFELLGLENLVQLFTCVLLEMQILLYSQDYQRLMTVAEGITSLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHFIELPEEFPQFPNKLEFIQEISEVLLQYGIPPEGNLHCSESATKLKSLALDDWVNDKKNGNLSGNNINMYELLKGNETIARLQALTKRTGITMDKINLAAPLVDAEDEQKVQCAEKELQDYKLNVQLREVFANRFTQMFSDYEAFVIQSAHDMESWLNNREQMQNFDKASFLSDQPEPYLPFLSRFIETQMFATFIDNKIMSLWDEKEPLLRVFDSRIQKLRLYNGRAPALRSSNYKKCTALKEAAQAIEQRLTKIDHTAIHPHLLDMKIGQGKYEQGFFPKLQSDVLMTGPTNNNRWVSRSATAQRRKDKLRQHSEHLGLDNDLREPSEEIVIRQNSMAFWEWDQGSPPPARPPKKSYSECCLKYMQEARSLGKTVRQPKLSDLSPAVIAQTNSKFVEGLLKECRMKTKRMLVEKMGHEAVELGHGEANITGLEENTLIASLCDLLERIWSHGLLVKQGKSALWSHLVQFQEREERLERTAESPTAPGPDRRKSDTGVSLPSLRVSLIQDMRHIQNLSEIKTDVGRARGWIRLSLEKKLLSQHLKQLLTNQSLTRKLYKRYAFLRCEEEKEQFLYHLLSLNAVDYFCFTSVFTTIMVPYRVVIIPIKKLSNAITTSNPWICVSGELGDTGVMQIPKNLLEMTFESQNLGKMTTVQLGHDNSGLLAKWLVDCVVVRSEITGHTYKFPCGRWLGKGVDDGSLERVLIGELITPTSDDDLGKQSRTPPQQKSPTVARRLSITSLTGKNIKPNAGQIQEGIGEAINNIVKHFHKPEKERGSLTVLLCGESGLVSALEQLFHHGFKSARIFQKSVFIWDFIEKAVAYFETTDQIGDGEADHLIPRSSCKTFCHYVNAIVAAPRHIGKDGKFQLLVCLGARDHLLALWIPLLAECPAITRMYEENALLRDPMTVNSLIRVLQTLQDFNIVLEGSLVKGVDV

Target ORF information:

RefSeq Version	XM_031897715.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897715.1

ORF Insert Sequence:

ATGAGCGGCC CCTCGGGCAC GGCCGGACCC CCCTGCCGCC TGGCTCATTA CTTCGTTATC 
TGCGGGACGG ACGCGGACAG TGGGCTCGAA CCGGATGAAC TGGCGGGGGA AAACTTTGAG 
CAGAGTCCAC TGAGGAGAAC CTTCAAGTCT AAAGTTCTGG CCCACTATCC GCAGAACGTC 
GAGTGGAGTC CATTCGATCA AGATGCTGTC AACATGCTGT GCATGCCCAA GGGCCTATCG 
TTCCGGACAC AGGCCGACGA CAGGGACCCG GAGTTCCATT CGTTCATCAT CACGCGGGAG 
GACGGCTCGC GCACCTACGG CTTCGTGCTG ACCTTTTACG AGGAGGTGAC GAGCCGGCAG 
ATCTGCGCCG CCATGCAGAC GCTGTACCAG ATGCACAACG CGGAGCAGAG TAACAGCGTC 
TACGCCTCGT CGTCGTGCAG CATGGACTCG CTGGCGAGCA GCGCGGACGA GAGCGACGAC 
GCCTCATTGG CCAAGTCCCA GCGCTACAAC TCGTACGACA TCAGTAAGGA CACGCTGTAC 
GTCTCCAAGT GCATCTGTCT GATCACGCCG CTGCCGTTCA TGCAGTCGTG CAGGAAGTTC 
CTGACGCAGT TGTACAAGGC GGTCACCTCG CCGCAGCCCC CGCCGCTGCC GCTGGAGAGC 
TACATCCATA ACATTCTGTA CGAGGTGCCG CTGCCCCCCC CGGGGAGGTC GCTCAAGTTC 
TACGGGGTAT ACGAGGCTAT TGTGTGCCAG AGGCCGGGGC CCAGCGAGCT GCCCCTCTCC 
GACTACCCCC TCAGGGAGAC CTTTGAGTTG TTGGGACTGG AAAACCTCGT GCAGCTTTTT 
ACCTGCGTCC TGCTGGAAAT GCAGATCCTG CTCTATTCCC AAGACTACCA GCGGCTAATG 
ACAGTGGCAG AGGGCATCAC CTCTCTCCTA TTCCCCTTTC AATGGCAGCA CGTCTACGTT 
CCCATCCTAC CCGCCTCCCT TCTGCATTTC CTCGATGCGC CTGTTCCGTA CCTGATGGGT 
CTTCAGTCCA AAGAAGGGAC CGACCGCTCC AAACTTGAAC TTCCTCAAGA GGCCAACCTG 
TGCTTTGTGG ATATCGATAA CCATTTCATC GAGCTGCCCG AGGAGTTCCC CCAGTTTCCC 
AACAAGCTGG AATTCATTCA GGAAATCTCG GAGGTCCTGC TGCAATACGG GATCCCCCCC 
GAGGGCAACT TGCACTGCAG CGAGAGCGCC ACCAAGCTGA AGAGCCTGGC CCTGGACGAC 
TGGGTCAACG ACAAGAAGAA CGGCAACCTC TCCGGCAACA ACATCAACAT GTACGAACTG 
CTAAAGGGCA ACGAGACCAT CGCCCGGCTC CAGGCGCTGA CTAAGCGGAC GGGGATAACC 
ATGGATAAAA TCAACCTGGC GGCGCCCCTA GTGGACGCGG AGGACGAGCA GAAAGTCCAA 
TGCGCCGAGA AGGAGCTTCA GGACTACAAA CTCAACGTGC AGCTCCGGGA GGTCTTCGCC 
AATCGCTTCA CACAGATGTT CTCCGACTAC GAGGCCTTCG TGATTCAGTC GGCCCACGAT 
ATGGAGTCGT GGCTCAACAA CAGGGAGCAG ATGCAGAACT TCGACAAGGC TTCCTTTCTG 
TCCGACCAGC CGGAGCCGTA CCTGCCGTTC CTGTCCCGTT TCATCGAGAC GCAGATGTTT 
GCCACCTTCA TAGACAATAA AATTATGTCC CTGTGGGACG AGAAGGAGCC GCTGCTGCGC 
GTGTTCGATT CCCGCATCCA GAAGTTGAGG CTGTACAACG GCCGCGCCCC CGCGCTCAGG 
TCCTCCAATT ATAAGAAATG CACCGCCCTG AAGGAGGCAG CCCAGGCCAT CGAGCAGCGG 
CTTACCAAGA TCGATCATAC GGCCATCCAC CCCCACCTGC TGGATATGAA GATCGGCCAG 
GGCAAGTACG AGCAGGGCTT CTTCCCCAAG CTGCAGTCCG ACGTCCTGAT GACCGGACCG 
ACCAATAACA ACCGATGGGT GAGCCGTAGT GCGACGGCGC AGCGGAGGAA AGACAAACTG 
CGGCAGCACT CTGAGCACCT GGGCCTAGAC AATGACTTAA GGGAGCCCTC TGAGGAGATA 
GTGATCCGGC AGAACTCTAT GGCTTTCTGG GAGTGGGATC AGGGGTCCCC CCCTCCAGCA 
CGGCCTCCAA AAAAATCCTA CTCTGAGTGC TGCCTGAAAT ACATGCAGGA AGCCAGGAGT 
CTGGGCAAAA CTGTCAGGCA ACCGAAATTA TCTGATCTCT CCCCGGCGGT TATTGCTCAG 
ACTAACTCCA AGTTTGTTGA AGGATTATTA AAAGAATGTC GTATGAAGAC AAAGCGGATG 
TTGGTGGAAA AAATGGGCCA CGAAGCTGTG GAGTTGGGCC ACGGAGAGGC CAATATAACT 
GGGTTAGAAG AGAACACACT GATTGCCAGC CTGTGTGACC TGTTAGAGAG AATATGGAGT 
CACGGCCTGC TTGTTAAACA GGGCAAGTCG GCGCTGTGGT CGCACCTGGT TCAGTTCCAG 
GAGAGAGAGG AGCGATTGGA GCGCACAGCG GAGTCGCCAA CGGCCCCAGG GCCAGACAGG 
AGGAAGTCAG ATACCGGCGT CAGTTTGCCG TCTCTACGAG TGTCGCTCAT CCAAGACATG 
AGGCATATCC AGAACCTGAG TGAGATCAAG ACGGACGTGG GCCGAGCTCG CGGGTGGATA 
CGGTTGTCCC TGGAGAAGAA GCTCCTGTCT CAGCACCTCA AGCAGCTTCT GACCAATCAG 
TCCCTCACCA GGAAGCTGTA CAAGCGCTAC GCATTTCTCC GCTGCGAGGA GGAGAAGGAG 
CAGTTTCTCT ACCATCTCCT GTCTCTCAAT GCCGTGGATT ACTTCTGCTT CACCAGCGTC 
TTTACCACTA TCATGGTTCC TTACAGGGTT GTGATCATCC CCATCAAAAA ACTGAGCAAC 
GCCATCACCA CGTCCAACCC GTGGATTTGT GTGTCGGGGG AGCTGGGGGA CACCGGGGTC 
ATGCAGATTC CCAAAAACCT GCTGGAGATG ACCTTTGAGA GCCAGAATTT GGGCAAAATG 
ACTACAGTTC AGCTGGGTCA CGACAACTCC GGCCTTTTGG CCAAGTGGCT CGTCGACTGC 
GTCGTGGTCC GAAGTGAGAT CACGGGACAC ACGTACAAGT TCCCGTGTGG CCGCTGGCTG 
GGGAAAGGGG TAGATGACGG GAGCCTAGAA AGAGTTTTGA TTGGTGAACT GATAACCCCC 
ACGTCGGATG ATGATCTTGG GAAACAAAGC CGCACCCCTC CCCAGCAGAA ATCTCCCACC 
GTGGCGCGCA GACTAAGCAT AACCTCCCTT ACAGGAAAAA ATATCAAACC AAATGCTGGG 
CAGATCCAGG AAGGTATCGG AGAAGCCATA AACAACATAG TGAAACATTT CCACAAACCT 
GAGAAAGAGA GAGGCAGCCT GACCGTCCTG CTGTGCGGAG AGAGTGGCCT CGTCTCGGCT 
CTGGAGCAGC TCTTTCACCA CGGATTCAAA TCCGCTCGGA TATTCCAGAA AAGCGTCTTC 
ATCTGGGACT TCATAGAGAA GGCCGTGGCT TACTTTGAAA CTACCGACCA AATAGGGGAC 
GGTGAAGCGG ATCATCTGAT CCCGAGGTCG TCCTGCAAGA CCTTCTGCCA CTACGTGAAT 
GCGATCGTGG CAGCCCCCCG GCACATTGGA AAAGATGGGA AATTCCAGCT CTTGGTCTGC 
CTAGGGGCAA GGGATCACTT ACTGGCCCTC TGGATCCCCC TGCTGGCTGA GTGCCCGGCC 
ATCACCAGGA TGTACGAGGA GAACGCCCTT CTCCGAGACC CCATGACGGT CAACTCGCTC 
ATACGGGTGC TGCAGACCTT ACAGGATTTC AACATCGTGC TGGAGGGCTC CCTTGTTAAA 
GGCGTCGACG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5b ( XM_031897716.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753576.1 Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), transcript variant X3, mRNA.

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CloneID	OXa53363
Clone ID Related Accession (Same CDS sequence)	XM_031897716.1
Accession Version	XM_031897716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DENN domain-containing protein 5B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCC CCTCGGGCAC GGCCGGACCC CCCTGCCGCC TGGCTCATTA CTTCGTTATC 
TGCGGGACGG ACGCGGACAG TGGGCTCGAA CCGGATGAAC TGGCGGTTTT GTACCAGTGG 
CTCGAAGCAG ACCGGCATGG CAAGGGCCAC GACACAGGGG AAAACTTTGA GCAGAGTCCA 
CTGAGGAGAA CCTTCAAGTC TAAAGTTCTG GCCCACTATC CGCAGAACGT CGAGTGGAGT 
CCATTCGATC AAGATGCTGT CAACATGCTG TGCATGCCCA AGGGCCTATC GTTCCGGACA 
CAGGCCGACG ACAGGGACCC GGAGTTCCAT TCGTTCATCA TCACGCGGGA GGACGGCTCG 
CGCACCTACG GCTTCGTGCT GACCTTTTAC GAGGAGGTGA CGAGCCGGCA GATCTGCGCC 
GCCATGCAGA CGCTGTACCA GATGCACAAC GCGGAGCAGA GTAACAGCGT CTACGCCTCG 
TCGTCGTGCA GCATGGACTC GCTGGCGAGC AGCGCGGACG AGAGCGACGA CGCCTCATTG 
GCCAAGTCCC AGCGCTACAA CTCGTACGAC ATCAGTAAGG ACACGCTGTA CGTCTCCAAG 
TGCATCTGTC TGATCACGCC GCTGCCGTTC ATGCAGTCGT GCAGGAAGTT CCTGACGCAG 
TTGTACAAGG CGGTCACCTC GCCGCAGCCC CCGCCGCTGC CGCTGGAGAG CTACATCCAT 
AACATTCTGT ACGAGGTGCC GCTGCCCCCC CCGGGGAGGT CGCTCAAGTT CTACGGGGTA 
TACGAGGCTA TTGTGTGCCA GAGGCCGGGG CCCAGCGAGC TGCCCCTCTC CGACTACCCC 
CTCAGGGAGA CCTTTGAGTT GTTGGGACTG GAAAACCTCG TGCAGCTTTT TACCTGCGTC 
CTGCTGGAAA TGCAGATCCT GCTCTATTCC CAAGACTACC AGCGGCTAAT GACAGTGGCA 
GAGGGCATCA CCTCTCTCCT ATTCCCCTTT CAATGGCAGC ACGTCTACGT TCCCATCCTA 
CCCGCCTCCC TTCTGCATTT CCTCGATGCG CCTGTTCCGT ACCTGATGGG TCTTCAGTCC 
AAAGAAGGGA CCGACCGCTC CAAACTTGAA CTTCCTCAAG AGGCCAACCT GTGCTTTGTG 
GATATCGATA ACCATTTCAT CGAGCTGCCC GAGGAGTTCC CCCAGTTTCC CAACAAGCTG 
GAATTCATTC AGGAAATCTC GGAGGTCCTG CTGCAATACG GGATCCCCCC CGAGGGCAAC 
TTGCACTGCA GCGAGAGCGC CACCAAGCTG AAGAGCCTGG CCCTGGACGA CTGGGTCAAC 
GACAAGAAGA ACGGCAACCT CTCCGGCAAC AACATCAACA TGTACGAACT GCTAAAGGGC 
AACGAGACCA TCGCCCGGCT CCAGGCGCTG ACTAAGCGGA CGGGGATAAC CATGGATAAA 
ATCAACCTGG CGGCGCCCCT AGTGGACGCG GAGGACGAGC AGAAAGTCCA ATGCGCCGAG 
AAGGAGCTTC AGGACTACAA ACTCAACGTG CAGCTCCGGG AGGTCTTCGC CAATCGCTTC 
ACACAGATGT TCTCCGACTA CGAGGCCTTC GTGATTCAGT CGGCCCACGA TATGGAGTCG 
TGGCTCAACA ACAGGGAGCA GATGCAGAAC TTCGACAAGG CTTCCTTTCT GTCCGACCAG 
CCGGAGCCGT ACCTGCCGTT CCTGTCCCGT TTCATCGAGA CGCAGATGTT TGCCACCTTC 
ATAGACAATA AAATTATGTC CCTGTGGGAC GAGAAGGAGC CGCTGCTGCG CGTGTTCGAT 
TCCCGCATCC AGAAGTTGAG GCTGTACAAC GGCCGCGCCC CCGCGCTCAG GTCCTCCAAT 
TATAAGAAAT GCACCGCCCT GAAGGAGGCA GCCCAGGCCA TCGAGCAGCG GCTTACCAAG 
ATCGATCATA CGGCCATCCA CCCCCACCTG CTGGATATGA AGATCGGCCA GGGCAAGTAC 
GAGCAGGGCT TCTTCCCCAA GCTGCAGTCC GACGTCCTGA TGACCGGACC GACCAATAAC 
AACCGATGGG TGAGCCGTAG TGCGACGGCG CAGCGGAGGA AAGACAAACT GCGGCAGCAC 
TCTGAGCACC TGGGCCTAGA CAATGACTTA AGGGAGAAAT ACATGCAGGA AGCCAGGAGT 
CTGGGCAAAA CTGTCAGGCA ACCGAAATTA TCTGATCTCT CCCCGGCGGT TATTGCTCAG 
ACTAACTCCA AGTTTGTTGA AGGATTATTA AAAGAATGTC GTATGAAGAC AAAGCGGATG 
TTGGTGGAAA AAATGGGCCA CGAAGCTGTG GAGTTGGGCC ACGGAGAGGC CAATATAACT 
GGGTTAGAAG AGAACACACT GATTGCCAGC CTGTGTGACC TGTTAGAGAG AATATGGAGT 
CACGGCCTGC TTGTTAAACA GGGCAAGTCG GCGCTGTGGT CGCACCTGGT TCAGTTCCAG 
GAGAGAGAGG AGCGATTGGA GCGCACAGCG GAGTCGCCAA CGGCCCCAGG GCCAGACAGG 
AGGAAGTCAG ATACCGGCGT CAGTTTGCCG TCTCTACGAG TGTCGCTCAT CCAAGACATG 
AGGCATATCC AGAACCTGAG TGAGATCAAG ACGGACGTGG GCCGAGCTCG CGGGTGGATA 
CGGTTGTCCC TGGAGAAGAA GCTCCTGTCT CAGCACCTCA AGCAGCTTCT GACCAATCAG 
TCCCTCACCA GGAAGCTGTA CAAGCGCTAC GCATTTCTCC GCTGCGAGGA GGAGAAGGAG 
CAGTTTCTCT ACCATCTCCT GTCTCTCAAT GCCGTGGATT ACTTCTGCTT CACCAGCGTC 
TTTACCACTA TCATGGTTCC TTACAGGGTT GTGATCATCC CCATCAAAAA ACTGAGCAAC 
GCCATCACCA CGTCCAACCC GTGGATTTGT GTGTCGGGGG AGCTGGGGGA CACCGGGGTC 
ATGCAGATTC CCAAAAACCT GCTGGAGATG ACCTTTGAGA GCCAGAATTT GGGCAAAATG 
ACTACAGTTC AGCTGGGTCA CGACAACTCC GGCCTTTTGG CCAAGTGGCT CGTCGACTGC 
GTCGTGGTCC GAAGTGAGAT CACGGGACAC ACGTACAAGT TCCCGTGTGG CCGCTGGCTG 
GGGAAAGGGG TAGATGACGG GAGCCTAGAA AGAGTTTTGA TTGGTGAACT GATAACCCCC 
ACGTCGGATG ATGATCTTGG GAAACAAAGC CGCACCCCTC CCCAGCAGAA ATCTCCCACC 
GTGGCGCGCA GACTAAGCAT AACCTCCCTT ACAGGAAAAA ATATCAAACC AAATGCTGGG 
CAGATCCAGG AAGGTATCGG AGAAGCCATA AACAACATAG TGAAACATTT CCACAAACCT 
GAGAAAGAGA GAGGCAGCCT GACCGTCCTG CTGTGCGGAG AGAGTGGCCT CGTCTCGGCT 
CTGGAGCAGC TCTTTCACCA CGGATTCAAA TCCGCTCGGA TATTCCAGAA AAGCGTCTTC 
ATCTGGGACT TCATAGAGAA GGCCGTGGCT TACTTTGAAA CTACCGACCA AATAGGGGAC 
GGTGAAGCGG ATCATCTGAT CCCGAGGTCG TCCTGCAAGA CCTTCTGCCA CTACGTGAAT 
GCGATCGTGG CAGCCCCCCG GCACATTGGA AAAGATGGGA AATTCCAGCT CTTGGTCTGC 
CTAGGGGCAA GGGATCACTT ACTGGCCCTC TGGATCCCCC TGCTGGCTGA GTGCCCGGCC 
ATCACCAGGA TGTACGAGGA GAACGCCCTT CTCCGAGACC CCATGACGGT CAACTCGCTC 
ATACGGGTGC TGCAGACCTT ACAGGATTTC AACATCGTGC TGGAGGGCTC CCTTGTTAAA 
GGCGTCGACG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753576.1
CDS	191..4045
Translation	MSGPSGTAGPPCRLAHYFVICGTDADSGLEPDELAVLYQWLEADRHGKGHDTGENFEQSPLRRTFKSKVLAHYPQNVEWSPFDQDAVNMLCMPKGLSFRTQADDRDPEFHSFIITREDGSRTYGFVLTFYEEVTSRQICAAMQTLYQMHNAEQSNSVYASSSCSMDSLASSADESDDASLAKSQRYNSYDISKDTLYVSKCICLITPLPFMQSCRKFLTQLYKAVTSPQPPPLPLESYIHNILYEVPLPPPGRSLKFYGVYEAIVCQRPGPSELPLSDYPLRETFELLGLENLVQLFTCVLLEMQILLYSQDYQRLMTVAEGITSLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHFIELPEEFPQFPNKLEFIQEISEVLLQYGIPPEGNLHCSESATKLKSLALDDWVNDKKNGNLSGNNINMYELLKGNETIARLQALTKRTGITMDKINLAAPLVDAEDEQKVQCAEKELQDYKLNVQLREVFANRFTQMFSDYEAFVIQSAHDMESWLNNREQMQNFDKASFLSDQPEPYLPFLSRFIETQMFATFIDNKIMSLWDEKEPLLRVFDSRIQKLRLYNGRAPALRSSNYKKCTALKEAAQAIEQRLTKIDHTAIHPHLLDMKIGQGKYEQGFFPKLQSDVLMTGPTNNNRWVSRSATAQRRKDKLRQHSEHLGLDNDLREKYMQEARSLGKTVRQPKLSDLSPAVIAQTNSKFVEGLLKECRMKTKRMLVEKMGHEAVELGHGEANITGLEENTLIASLCDLLERIWSHGLLVKQGKSALWSHLVQFQEREERLERTAESPTAPGPDRRKSDTGVSLPSLRVSLIQDMRHIQNLSEIKTDVGRARGWIRLSLEKKLLSQHLKQLLTNQSLTRKLYKRYAFLRCEEEKEQFLYHLLSLNAVDYFCFTSVFTTIMVPYRVVIIPIKKLSNAITTSNPWICVSGELGDTGVMQIPKNLLEMTFESQNLGKMTTVQLGHDNSGLLAKWLVDCVVVRSEITGHTYKFPCGRWLGKGVDDGSLERVLIGELITPTSDDDLGKQSRTPPQQKSPTVARRLSITSLTGKNIKPNAGQIQEGIGEAINNIVKHFHKPEKERGSLTVLLCGESGLVSALEQLFHHGFKSARIFQKSVFIWDFIEKAVAYFETTDQIGDGEADHLIPRSSCKTFCHYVNAIVAAPRHIGKDGKFQLLVCLGARDHLLALWIPLLAECPAITRMYEENALLRDPMTVNSLIRVLQTLQDFNIVLEGSLVKGVDV

Target ORF information:

RefSeq Version	XM_031897716.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 5B (dennd5b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897716.1

ORF Insert Sequence:

ATGAGCGGCC CCTCGGGCAC GGCCGGACCC CCCTGCCGCC TGGCTCATTA CTTCGTTATC 
TGCGGGACGG ACGCGGACAG TGGGCTCGAA CCGGATGAAC TGGCGGTTTT GTACCAGTGG 
CTCGAAGCAG ACCGGCATGG CAAGGGCCAC GACACAGGGG AAAACTTTGA GCAGAGTCCA 
CTGAGGAGAA CCTTCAAGTC TAAAGTTCTG GCCCACTATC CGCAGAACGT CGAGTGGAGT 
CCATTCGATC AAGATGCTGT CAACATGCTG TGCATGCCCA AGGGCCTATC GTTCCGGACA 
CAGGCCGACG ACAGGGACCC GGAGTTCCAT TCGTTCATCA TCACGCGGGA GGACGGCTCG 
CGCACCTACG GCTTCGTGCT GACCTTTTAC GAGGAGGTGA CGAGCCGGCA GATCTGCGCC 
GCCATGCAGA CGCTGTACCA GATGCACAAC GCGGAGCAGA GTAACAGCGT CTACGCCTCG 
TCGTCGTGCA GCATGGACTC GCTGGCGAGC AGCGCGGACG AGAGCGACGA CGCCTCATTG 
GCCAAGTCCC AGCGCTACAA CTCGTACGAC ATCAGTAAGG ACACGCTGTA CGTCTCCAAG 
TGCATCTGTC TGATCACGCC GCTGCCGTTC ATGCAGTCGT GCAGGAAGTT CCTGACGCAG 
TTGTACAAGG CGGTCACCTC GCCGCAGCCC CCGCCGCTGC CGCTGGAGAG CTACATCCAT 
AACATTCTGT ACGAGGTGCC GCTGCCCCCC CCGGGGAGGT CGCTCAAGTT CTACGGGGTA 
TACGAGGCTA TTGTGTGCCA GAGGCCGGGG CCCAGCGAGC TGCCCCTCTC CGACTACCCC 
CTCAGGGAGA CCTTTGAGTT GTTGGGACTG GAAAACCTCG TGCAGCTTTT TACCTGCGTC 
CTGCTGGAAA TGCAGATCCT GCTCTATTCC CAAGACTACC AGCGGCTAAT GACAGTGGCA 
GAGGGCATCA CCTCTCTCCT ATTCCCCTTT CAATGGCAGC ACGTCTACGT TCCCATCCTA 
CCCGCCTCCC TTCTGCATTT CCTCGATGCG CCTGTTCCGT ACCTGATGGG TCTTCAGTCC 
AAAGAAGGGA CCGACCGCTC CAAACTTGAA CTTCCTCAAG AGGCCAACCT GTGCTTTGTG 
GATATCGATA ACCATTTCAT CGAGCTGCCC GAGGAGTTCC CCCAGTTTCC CAACAAGCTG 
GAATTCATTC AGGAAATCTC GGAGGTCCTG CTGCAATACG GGATCCCCCC CGAGGGCAAC 
TTGCACTGCA GCGAGAGCGC CACCAAGCTG AAGAGCCTGG CCCTGGACGA CTGGGTCAAC 
GACAAGAAGA ACGGCAACCT CTCCGGCAAC AACATCAACA TGTACGAACT GCTAAAGGGC 
AACGAGACCA TCGCCCGGCT CCAGGCGCTG ACTAAGCGGA CGGGGATAAC CATGGATAAA 
ATCAACCTGG CGGCGCCCCT AGTGGACGCG GAGGACGAGC AGAAAGTCCA ATGCGCCGAG 
AAGGAGCTTC AGGACTACAA ACTCAACGTG CAGCTCCGGG AGGTCTTCGC CAATCGCTTC 
ACACAGATGT TCTCCGACTA CGAGGCCTTC GTGATTCAGT CGGCCCACGA TATGGAGTCG 
TGGCTCAACA ACAGGGAGCA GATGCAGAAC TTCGACAAGG CTTCCTTTCT GTCCGACCAG 
CCGGAGCCGT ACCTGCCGTT CCTGTCCCGT TTCATCGAGA CGCAGATGTT TGCCACCTTC 
ATAGACAATA AAATTATGTC CCTGTGGGAC GAGAAGGAGC CGCTGCTGCG CGTGTTCGAT 
TCCCGCATCC AGAAGTTGAG GCTGTACAAC GGCCGCGCCC CCGCGCTCAG GTCCTCCAAT 
TATAAGAAAT GCACCGCCCT GAAGGAGGCA GCCCAGGCCA TCGAGCAGCG GCTTACCAAG 
ATCGATCATA CGGCCATCCA CCCCCACCTG CTGGATATGA AGATCGGCCA GGGCAAGTAC 
GAGCAGGGCT TCTTCCCCAA GCTGCAGTCC GACGTCCTGA TGACCGGACC GACCAATAAC 
AACCGATGGG TGAGCCGTAG TGCGACGGCG CAGCGGAGGA AAGACAAACT GCGGCAGCAC 
TCTGAGCACC TGGGCCTAGA CAATGACTTA AGGGAGAAAT ACATGCAGGA AGCCAGGAGT 
CTGGGCAAAA CTGTCAGGCA ACCGAAATTA TCTGATCTCT CCCCGGCGGT TATTGCTCAG 
ACTAACTCCA AGTTTGTTGA AGGATTATTA AAAGAATGTC GTATGAAGAC AAAGCGGATG 
TTGGTGGAAA AAATGGGCCA CGAAGCTGTG GAGTTGGGCC ACGGAGAGGC CAATATAACT 
GGGTTAGAAG AGAACACACT GATTGCCAGC CTGTGTGACC TGTTAGAGAG AATATGGAGT 
CACGGCCTGC TTGTTAAACA GGGCAAGTCG GCGCTGTGGT CGCACCTGGT TCAGTTCCAG 
GAGAGAGAGG AGCGATTGGA GCGCACAGCG GAGTCGCCAA CGGCCCCAGG GCCAGACAGG 
AGGAAGTCAG ATACCGGCGT CAGTTTGCCG TCTCTACGAG TGTCGCTCAT CCAAGACATG 
AGGCATATCC AGAACCTGAG TGAGATCAAG ACGGACGTGG GCCGAGCTCG CGGGTGGATA 
CGGTTGTCCC TGGAGAAGAA GCTCCTGTCT CAGCACCTCA AGCAGCTTCT GACCAATCAG 
TCCCTCACCA GGAAGCTGTA CAAGCGCTAC GCATTTCTCC GCTGCGAGGA GGAGAAGGAG 
CAGTTTCTCT ACCATCTCCT GTCTCTCAAT GCCGTGGATT ACTTCTGCTT CACCAGCGTC 
TTTACCACTA TCATGGTTCC TTACAGGGTT GTGATCATCC CCATCAAAAA ACTGAGCAAC 
GCCATCACCA CGTCCAACCC GTGGATTTGT GTGTCGGGGG AGCTGGGGGA CACCGGGGTC 
ATGCAGATTC CCAAAAACCT GCTGGAGATG ACCTTTGAGA GCCAGAATTT GGGCAAAATG 
ACTACAGTTC AGCTGGGTCA CGACAACTCC GGCCTTTTGG CCAAGTGGCT CGTCGACTGC 
GTCGTGGTCC GAAGTGAGAT CACGGGACAC ACGTACAAGT TCCCGTGTGG CCGCTGGCTG 
GGGAAAGGGG TAGATGACGG GAGCCTAGAA AGAGTTTTGA TTGGTGAACT GATAACCCCC 
ACGTCGGATG ATGATCTTGG GAAACAAAGC CGCACCCCTC CCCAGCAGAA ATCTCCCACC 
GTGGCGCGCA GACTAAGCAT AACCTCCCTT ACAGGAAAAA ATATCAAACC AAATGCTGGG 
CAGATCCAGG AAGGTATCGG AGAAGCCATA AACAACATAG TGAAACATTT CCACAAACCT 
GAGAAAGAGA GAGGCAGCCT GACCGTCCTG CTGTGCGGAG AGAGTGGCCT CGTCTCGGCT 
CTGGAGCAGC TCTTTCACCA CGGATTCAAA TCCGCTCGGA TATTCCAGAA AAGCGTCTTC 
ATCTGGGACT TCATAGAGAA GGCCGTGGCT TACTTTGAAA CTACCGACCA AATAGGGGAC 
GGTGAAGCGG ATCATCTGAT CCCGAGGTCG TCCTGCAAGA CCTTCTGCCA CTACGTGAAT 
GCGATCGTGG CAGCCCCCCG GCACATTGGA AAAGATGGGA AATTCCAGCT CTTGGTCTGC 
CTAGGGGCAA GGGATCACTT ACTGGCCCTC TGGATCCCCC TGCTGGCTGA GTGCCCGGCC 
ATCACCAGGA TGTACGAGGA GAACGCCCTT CTCCGAGACC CCATGACGGT CAACTCGCTC 
ATACGGGTGC TGCAGACCTT ACAGGATTTC AACATCGTGC TGGAGGGCTC CCTTGTTAAA 
GGCGTCGACG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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dennd5b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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