map3k10.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	map3k10.L
Entrez Gene ID	398612
Full Name	mitogen-activated protein kinase kinase kinase 10 L homeolog
Synonyms	map3k10,mlk2,xmlk2
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 10 L homeolog Names mitogen-activated protein kinase kinase kinase 10 mixed lineage kinase 2
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018228738.1	XP_018084227.1	mitogen-activated protein kinase kinase kinase 10 isoform X1
NM_001089160.1	NP_001082629.1	mitogen-activated protein kinase kinase kinase 10

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

PAK interacts with NCK and MLK2 to regulate the activation of jun N-terminal kinase.
Poitras L, Jean S, Islam N, Moss T
FEBS letters543(1-3)129-35(2003 May)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following map3k10.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map3k10.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf41014	XM_018228738.1 Latest version!	Xenopus laevis mitogen-activated protein kinase kinase kinase 10 L homeolog (map3k10.L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAf01141	NM_001089160.1 Latest version!	Xenopus laevis mitogen-activated protein kinase kinase kinase 10 L homeolog (map3k10.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

map3k10.L ( XM_018228738.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018084227.1 Xenopus laevis mitogen-activated protein kinase kinase kinase 10 L homeolog (map3k10.L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf41014
Clone ID Related Accession (Same CDS sequence)	XM_018228738.1
Accession Version	XM_018228738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	mitogen-activated protein kinase kinase kinase 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030738.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATG GACTTCCGAA AAATGAGGCC TTTCTATGGC AGAGCTCAAA AGACAATAAA 
GAAAATGGGG TGTGGAGTGA TGTGCAGAGT TATGGGGTCT CCAATCCATT ATGGATGGCA 
GTTTTCGATT ATGAAGCAAC AGCAGAAGAG GAATTAACCT TACGGAGGGG AGACTTGGTT 
GAGATACTCT CAAAGGACTC TACTGTTTCT GGTGATGAGG GTTGGTGGAC AGGAAAAATT 
AAAGACAAGG TGGGCATATT TCCAAGTAAT TATGTGGTGA GTGACGATAA ATACACTACC 
CTAACAGGAG CCCCAAAGCA ATGCCCTCTC CCACTAGAAA TCGAGTTTGA AGAGCTGAAC 
CTGGATGAAA TCATTGGGGT GGGAGGATTT GGGAAGGTCT ATAAGGGATT ATGGAGAGAT 
GAAGAGGTTG CAGTTAAAGC TGTCCGTCAT GACCCAGATG AAGATATCAA CGTGACTGCT 
GAGAATGTGC GACAGGAAGC CAAGCTCTTC TGTATGTTGT GCCACCCCAA CATCATAGCC 
CTGACAGGGG TGTGCCTAAA ACCGCCTCAT CTTTGTCTGG TTATGGAGTA TGCCAGAGGT 
GGACCATTAC ACAGAGCCCT GGCTGGGAAG AAGGTTCCAG CTCATGTATT GGTAAACTGG 
GCAGTGCAGA TAGCCAAAGG CATGACCTAT CTCCACAATG AAGCCATTGT TCCTATTATA 
CACAGGGATC TAAAATCCAG CAACATCTTA ATACTAGAGA AAGCAGAGAA TGATGACTTG 
TTTAACAAGA CACTGAAAAT CACAGATTTT GGGCTGGCCA GGGAGTGGCA GAAGACAACG 
AAGATGAGCG CAGCTGGTAC CTATGCTTGG ATGGCCCCAG AAGTTATCCG CTTATCTCTC 
TTTTCTAAGA GCAGTGATGT GTGGAGTTTT GGGGTTCTGC TCTGGGAGCT TCTAACAGGA 
GAGGTGCCAT ATCGGGAGAT AGATGCTCTT GCTGTAGCAT ATGGTGTGGC CATGAACAAG 
CTTACCTTAC CTATCCCATC GACTTGTCCT GAGCCTTTTG TACGGATCCT TGAAGCATGT 
TGGGATCCGG ACCCTCACAG CAGACCTACA TTTTCTTGTA TTCTGGAACA ACTGACAACT 
ATAGAACAGT CAGCCATGTT TCAAATGCCC TTGGAGTCCT TCCATTCTCT TCAGGAAGAC 
TGGAGGCTGG AAATCCAGCA GATGTTTGAT GAATTGAGGA CCAAAGAAAA GGAGCTGCGC 
AGCCGAGAGG AGGAGCTTGT GAGAGCAGCT GAGGAACAGA GGATTTTGGA GGATCTGCTG 
AAGAGGAGAG AGCAAGAGTT GGCTGAGAGG GAAATTGACA TTGTTGAGCG AGAGCTGAAC 
ATAATTATGT ATCAGATGTA CCAAGAAAAG CCAAAGGTGA AAAAACGCAA GGGCAATTTC 
AAGAAAAGCC GCCTAAAACT TAAGGATGGC AACCGGATCA GCTTGCCTTC AGGTTTCGAA 
CATAAGATTA CAGTCCAGGC ATCTCCAATG TTGGACAAAT GTAAGGGGCA GGGGACAAGC 
AGCTATAGCC CACCTGGGAG TCCACTAATA ATTCCGAGGC TGAGAGCCAT TAGATTAACA 
CCTGTTGATG GCAGTAAGAC ATGGGGGCGC AGTTCTGTAC TAAAGAAAGA AGAGGTAACA 
ACATCAAATA AGAAGAAAGG CAGGACTTGG GGACCTAGCT CGACACAGCA GAAAGAACGA 
GTAGGGGGAG AAGAAAGGCT GAAGACTCTT GGAGAAGGCA ATAAGCAGTG GTCAAGCAGT 
GCTCCCAACC TGGGCAAATC TCCTAAGCAT ACTCCCATCA GTGTGGGGTT TGCAAGCCTC 
ACTGAAATGG AGGAATATGC TGATTCTGAT GGCTCCGTCC CACAGTCTCC CTACTCACAG 
TCCTACCTCA CACTGCCTGT CCAATCAGAC CATAGAAGTC ATCCAGAGGA CACAGCGCAT 
GCTGGTGCAC CCTCATCAGA CAGCCCCAAA CGTGGTTCGC AGTCCCGTCG GAAAAGTGAA 
CTTGTGTTAC TGGGGTGTGC GTCTCTCTTA GCTGCTGTTG CTTTGGGCAG TGACTTATCA 
GAGCTGGTGC CTCAGGAAGA GAAAAGAAAG GGTATATTCC AATGGGCAGG GAGAGGTCCC 
AGGCGACGTG CTAGTTCTCC AAGTCGCTCT ATGTCCTATG GTGAAGACTC TGTCATTCCA 
TCCAGCTCAG TTACACTCAT TTCCTTGTCT TCCATCTCAG ACTGCAATTC CACAAGGTCT 
CTGATTCGAT CAGATAGTGA TGACATCGGA CTGGACCATG ATAACGTGTC TAGTGGGAGG 
GGGGTAAAAG AGGACCGAGG ACAGCAACCA AATGTTGGCA GCAACCCTCT GGTAGATTAT 
AAGGTTGAAA GTTTTAAGAG AGATCCAAAG CAGTCTCTGA CTCCCACCCA TGTGACAGTA 
GGAAGAAATA ACACAACTGA AACCAGAGGA CACAGGAGAA CCCCTTCCGA TGGAGCCATA 
AGGCAAGTGA CACAGGGTCA TAAACGCTCT CCATCTGACG GAAGCACCCC TTATCAATGT 
GAACCAGAAC CATCACCTTT CCCTCGCCTC CCTGATCCCC ATTTTGTGTT CCCTCCTCCA 
GTCCGACGTA AGGACACAGG AGTAGAAAGG CCAACATCTC TAGAATTTGC TCCCAGGCCT 
CGTCCGTCTT CAAACCGACC TCGAATGGAT CCCTGGAAGT TTGTTTCTCT TTCACAAACT 
CATAGCTCGT CCCCCTCTAG TGGTGGGGGT GATGCATGCT CCAGTGGATC AGCAGAGGGA 
GCTCAAGTGG CTGATGTAGA GGAGACACTG CTTGATATGG AGGTGGAAGG ACAAAGACTA 
GACAGCACAG TACCTTTGTG CGGACTGGGT TTAAGACCAA CAACTGACCC TTTTTTAAAT 
ATGGGAACAG ACGTGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018084227.1
CDS	1154..4114
Translation	MMDGLPKNEAFLWQSSKDNKENGVWSDVQSYGVSNPLWMAVFDYEATAEEELTLRRGDLVEILSKDSTVSGDEGWWTGKIKDKVGIFPSNYVVSDDKYTTLTGAPKQCPLPLEIEFEELNLDEIIGVGGFGKVYKGLWRDEEVAVKAVRHDPDEDINVTAENVRQEAKLFCMLCHPNIIALTGVCLKPPHLCLVMEYARGGPLHRALAGKKVPAHVLVNWAVQIAKGMTYLHNEAIVPIIHRDLKSSNILILEKAENDDLFNKTLKITDFGLAREWQKTTKMSAAGTYAWMAPEVIRLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFVRILEACWDPDPHSRPTFSCILEQLTTIEQSAMFQMPLESFHSLQEDWRLEIQQMFDELRTKEKELRSREEELVRAAEEQRILEDLLKRREQELAEREIDIVERELNIIMYQMYQEKPKVKKRKGNFKKSRLKLKDGNRISLPSGFEHKITVQASPMLDKCKGQGTSSYSPPGSPLIIPRLRAIRLTPVDGSKTWGRSSVLKKEEVTTSNKKKGRTWGPSSTQQKERVGGEERLKTLGEGNKQWSSSAPNLGKSPKHTPISVGFASLTEMEEYADSDGSVPQSPYSQSYLTLPVQSDHRSHPEDTAHAGAPSSDSPKRGSQSRRKSELVLLGCASLLAAVALGSDLSELVPQEEKRKGIFQWAGRGPRRRASSPSRSMSYGEDSVIPSSSVTLISLSSISDCNSTRSLIRSDSDDIGLDHDNVSSGRGVKEDRGQQPNVGSNPLVDYKVESFKRDPKQSLTPTHVTVGRNNTTETRGHRRTPSDGAIRQVTQGHKRSPSDGSTPYQCEPEPSPFPRLPDPHFVFPPPVRRKDTGVERPTSLEFAPRPRPSSNRPRMDPWKFVSLSQTHSSSPSSGGGDACSSGSAEGAQVADVEETLLDMEVEGQRLDSTVPLCGLGLRPTTDPFLNMGTDVS

Target ORF information:

RefSeq Version	XM_018228738.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis mitogen-activated protein kinase kinase kinase 10 L homeolog (map3k10.L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018228738.1

ORF Insert Sequence:

ATGATGGATG GACTTCCGAA AAATGAGGCC TTTCTATGGC AGAGCTCAAA AGACAATAAA 
GAAAATGGGG TGTGGAGTGA TGTGCAGAGT TATGGGGTCT CCAATCCATT ATGGATGGCA 
GTTTTCGATT ATGAAGCAAC AGCAGAAGAG GAATTAACCT TACGGAGGGG AGACTTGGTT 
GAGATACTCT CAAAGGACTC TACTGTTTCT GGTGATGAGG GTTGGTGGAC AGGAAAAATT 
AAAGACAAGG TGGGCATATT TCCAAGTAAT TATGTGGTGA GTGACGATAA ATACACTACC 
CTAACAGGAG CCCCAAAGCA ATGCCCTCTC CCACTAGAAA TCGAGTTTGA AGAGCTGAAC 
CTGGATGAAA TCATTGGGGT GGGAGGATTT GGGAAGGTCT ATAAGGGATT ATGGAGAGAT 
GAAGAGGTTG CAGTTAAAGC TGTCCGTCAT GACCCAGATG AAGATATCAA CGTGACTGCT 
GAGAATGTGC GACAGGAAGC CAAGCTCTTC TGTATGTTGT GCCACCCCAA CATCATAGCC 
CTGACAGGGG TGTGCCTAAA ACCGCCTCAT CTTTGTCTGG TTATGGAGTA TGCCAGAGGT 
GGACCATTAC ACAGAGCCCT GGCTGGGAAG AAGGTTCCAG CTCATGTATT GGTAAACTGG 
GCAGTGCAGA TAGCCAAAGG CATGACCTAT CTCCACAATG AAGCCATTGT TCCTATTATA 
CACAGGGATC TAAAATCCAG CAACATCTTA ATACTAGAGA AAGCAGAGAA TGATGACTTG 
TTTAACAAGA CACTGAAAAT CACAGATTTT GGGCTGGCCA GGGAGTGGCA GAAGACAACG 
AAGATGAGCG CAGCTGGTAC CTATGCTTGG ATGGCCCCAG AAGTTATCCG CTTATCTCTC 
TTTTCTAAGA GCAGTGATGT GTGGAGTTTT GGGGTTCTGC TCTGGGAGCT TCTAACAGGA 
GAGGTGCCAT ATCGGGAGAT AGATGCTCTT GCTGTAGCAT ATGGTGTGGC CATGAACAAG 
CTTACCTTAC CTATCCCATC GACTTGTCCT GAGCCTTTTG TACGGATCCT TGAAGCATGT 
TGGGATCCGG ACCCTCACAG CAGACCTACA TTTTCTTGTA TTCTGGAACA ACTGACAACT 
ATAGAACAGT CAGCCATGTT TCAAATGCCC TTGGAGTCCT TCCATTCTCT TCAGGAAGAC 
TGGAGGCTGG AAATCCAGCA GATGTTTGAT GAATTGAGGA CCAAAGAAAA GGAGCTGCGC 
AGCCGAGAGG AGGAGCTTGT GAGAGCAGCT GAGGAACAGA GGATTTTGGA GGATCTGCTG 
AAGAGGAGAG AGCAAGAGTT GGCTGAGAGG GAAATTGACA TTGTTGAGCG AGAGCTGAAC 
ATAATTATGT ATCAGATGTA CCAAGAAAAG CCAAAGGTGA AAAAACGCAA GGGCAATTTC 
AAGAAAAGCC GCCTAAAACT TAAGGATGGC AACCGGATCA GCTTGCCTTC AGGTTTCGAA 
CATAAGATTA CAGTCCAGGC ATCTCCAATG TTGGACAAAT GTAAGGGGCA GGGGACAAGC 
AGCTATAGCC CACCTGGGAG TCCACTAATA ATTCCGAGGC TGAGAGCCAT TAGATTAACA 
CCTGTTGATG GCAGTAAGAC ATGGGGGCGC AGTTCTGTAC TAAAGAAAGA AGAGGTAACA 
ACATCAAATA AGAAGAAAGG CAGGACTTGG GGACCTAGCT CGACACAGCA GAAAGAACGA 
GTAGGGGGAG AAGAAAGGCT GAAGACTCTT GGAGAAGGCA ATAAGCAGTG GTCAAGCAGT 
GCTCCCAACC TGGGCAAATC TCCTAAGCAT ACTCCCATCA GTGTGGGGTT TGCAAGCCTC 
ACTGAAATGG AGGAATATGC TGATTCTGAT GGCTCCGTCC CACAGTCTCC CTACTCACAG 
TCCTACCTCA CACTGCCTGT CCAATCAGAC CATAGAAGTC ATCCAGAGGA CACAGCGCAT 
GCTGGTGCAC CCTCATCAGA CAGCCCCAAA CGTGGTTCGC AGTCCCGTCG GAAAAGTGAA 
CTTGTGTTAC TGGGGTGTGC GTCTCTCTTA GCTGCTGTTG CTTTGGGCAG TGACTTATCA 
GAGCTGGTGC CTCAGGAAGA GAAAAGAAAG GGTATATTCC AATGGGCAGG GAGAGGTCCC 
AGGCGACGTG CTAGTTCTCC AAGTCGCTCT ATGTCCTATG GTGAAGACTC TGTCATTCCA 
TCCAGCTCAG TTACACTCAT TTCCTTGTCT TCCATCTCAG ACTGCAATTC CACAAGGTCT 
CTGATTCGAT CAGATAGTGA TGACATCGGA CTGGACCATG ATAACGTGTC TAGTGGGAGG 
GGGGTAAAAG AGGACCGAGG ACAGCAACCA AATGTTGGCA GCAACCCTCT GGTAGATTAT 
AAGGTTGAAA GTTTTAAGAG AGATCCAAAG CAGTCTCTGA CTCCCACCCA TGTGACAGTA 
GGAAGAAATA ACACAACTGA AACCAGAGGA CACAGGAGAA CCCCTTCCGA TGGAGCCATA 
AGGCAAGTGA CACAGGGTCA TAAACGCTCT CCATCTGACG GAAGCACCCC TTATCAATGT 
GAACCAGAAC CATCACCTTT CCCTCGCCTC CCTGATCCCC ATTTTGTGTT CCCTCCTCCA 
GTCCGACGTA AGGACACAGG AGTAGAAAGG CCAACATCTC TAGAATTTGC TCCCAGGCCT 
CGTCCGTCTT CAAACCGACC TCGAATGGAT CCCTGGAAGT TTGTTTCTCT TTCACAAACT 
CATAGCTCGT CCCCCTCTAG TGGTGGGGGT GATGCATGCT CCAGTGGATC AGCAGAGGGA 
GCTCAAGTGG CTGATGTAGA GGAGACACTG CTTGATATGG AGGTGGAAGG ACAAAGACTA 
GACAGCACAG TACCTTTGTG CGGACTGGGT TTAAGACCAA CAACTGACCC TTTTTTAAAT 
ATGGGAACAG ACGTGTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k10.L ( NM_001089160.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001082629.1 Xenopus laevis mitogen-activated protein kinase kinase kinase 10 L homeolog (map3k10.L), mRNA.

Price & Availability↑

CloneID	OAf01141
Clone ID Related Accession (Same CDS sequence)	NM_001089160.1
Accession Version	NM_001089160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577203200000
Organism	Xenopus laevis(African clawed frog)
Product	mitogen-activated protein kinase kinase kinase 10
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF510499.1. On Jul 25, 2007 this sequence version replaced NM_001089161.1. ##Evidence-Data-START## Transcript exon combination :: AF510499.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012420 [ECO:0000348] ##Evidence-Data-END##

ATGGATGGAC TTCCGAAAGA TGAGGCCTTT CTGTGGCAGA GCTCAAAAGA CAATAAAGAA 
AATGGGGTGT GGAGTGATGT GCAGAGTTAT GGGGTCTCCA ATCCACTATG GATGGCAGTT 
TTCGATTATG AACCAACAGC AGAAGAGGAA TTAACCTTAC GGAGGGGAGA CTTGGTTGAG 
ATTCTCTCAA AGGACTCTAC TGTTTCTGGT GATGAGGGTT GGTGGACAGG AAAAATTAAA 
GACAAGGTGG GCATATTTCC AAGTAATTAT GTGGTGAGTG ACGATAAATA CACTACCCTA 
ACAGGAGCCC CAAAGCAATG CCCTCTCCCA CTAGAAATCG AGTTTGATGA GCTGAACCTG 
GATGAAATCA TTGGGGTGGG AGGATTTGGG AAGGTCTATA AGGGATTATG GAGAGATGAA 
GAGGTTGCAG TTAAAGCTGT CCGTCATGAC CCAGATGAAG ATATCAACGT GACTGCTGAG 
AATGTGCGAC AGGAAGCCAA GATCTTCTGT ATGTTGTGCC ACCCCAACAT CATAGCCCTG 
ACAGGGGTGT GCCTAAAACC GCCTCATCTT TGTCTGGTTA TGGAGTATGC CAGAGGTGGA 
CCATTACACA GAGCCCTGGC TGGGAAGAAG GTTCCAGCTC ATGTATTGGT AAACTGGGCG 
GTGCAGATAG CCAAAGGCAT GACCTATCTC CACAATGAAG CCATTGTTCC TATTATACAC 
AGGGATCTGG GGTCCAGCAA CATCTTAATA CTAGAGAAAG CAGAGAATGA TGACTTGTTT 
AACAAGACAC TGAATATCAC AGATTTTGGG CTGGCCAGGG AGTGGCAGAA GACAACGAAG 
ATGAGCGCAG CTGGTACCTA TGCTTGGATG GCCCCAGAAG TTATCCGCTT ATCTCTCTTT 
TCTAAGAGCA GTGATGTGTG GAGTTTTGGG GTTCTGCTCT GGGAGCTTCT AACAGGAGAG 
GTGCCATATC GGGAGATAGA TGCTCTTGCT GTAGCATATG GTGTGGCCAT GAACAAGCTT 
ACCTTACCTA TCCCATCGAC TTGTCCTGAG CCTTTTGTAC GGATCCTTGA AGCATGTTGG 
GATCCGGACC CTCACAGCAG ACCTACATTT TCTTGTATTC TGGAACAACT GACAACTATA 
GAACAGTCAG CCATGTTTCA AATGCCCTTG GAGTCCTTCC ATTCTCTTCA GGAAGACTGG 
AGGCTGGAAA TCCAGCAGAT GTTTGATGAA TTGAGGACCA AAGAAAAGGA GCTGCGCAGC 
CGAGAGGAGG AGCTTGTGAG AGCAGCTGAG GAACAGAGGA TTTTGGAGGA TCTGCTGAAG 
AGGAGAGAGC AAGAGTTGGC TGAGAGGGAA ATTGACATTG TTGAGCGAGA GCTGAACATA 
ATTATGTATC AGATGTACCA AGAAAAGCCA AAGGTGAAAA AACGCAAGGG CAATTTCAAG 
AAAAGCCGCC TAAAACTTAA GGATGGCAAC CGGATCAGCT TGCCTTCAGG TTTCGAACAT 
AAGATTACAG TCCAGGCATC TCCAATGTTG GACAAATGTA AGGGGCAGGG GACAAGCAGC 
TATAGCCCAC CTGGGAGTCC ACTAATAATT CCGAGGCTGA GAGCCATTAG ATTAACACCT 
GTTGATGGCA GTAAGACATG GGGGCGCAGT TCTGTACTAA AGAAAGAAGA GGTAACAACA 
TCAAATAAGA AGAAAGGCAG GACTTGGGGA CCTAGCTCGA CACAGCAGAA AGAACGAGTA 
GGGGGAGAAG AAAGGCTGAA GACTCTTGGA GAAGGCAATA AGCAGTGGTC AAGCAGTGCT 
CCCAACCTGG GCAAATCTCC TAAGCATACT CCCATCAGTG TGGGGTTTGC AAGCCTCACT 
GAAATGGAGG AATATGCTGA TTCTGATGGC TCCGTCCCAC AGTCTCCCTA CTCACAGTCC 
TACCTCACAC TGCCTGTCCA ATCAGACCAT AGAAGTCATC CAGAGGACAC AGCGCATGCT 
GGTGCACCCT CATCAGACAG CCCCAAACGT GGTTCGCAGT CCCGTCGGAA AAGTGAACTT 
GTGTTACTGG GGTGTGCGTC TCTCTTAGCT GCTGTTGCTT TGGGCAGTGA CTTATCAGAG 
CTGGTGCCTC AGGAAGAGAA AAGAAAGGGT ATATTCCAAT GGGCAGGGAG AGGTCCCAGG 
CGACGTGCTA GTTCTCCAAG TCGCTCTATG TCCTATGGTG AAGACTCTGT CATTCCATCC 
AGCTCAGTTA CACTCATTTC CTTGTCTTCC ATCTCAGACT GCAATTCCAC AAGGTCTCTG 
ATTCGATCAG ATAGTGATGA CATCGGACTG GACCATGATA ACGTGTCTAG TGGGAGGGGG 
GTAAAAGAGG ACCGAGGACA GCAACCAAAT GTTGGCAGCA ACCCTCTGGT AGATTATAAG 
GTTGAAAGTT TTAAGAGAGA TCCAAAGCAG TCTCTGACTC CCACCCATGT GACAGTAGGA 
AGAAATAACA CAACTGAAAC CAGAGGACAC AGGAGAACCC CTTCCGATGG AGCCATAAGG 
CAAGTGACAC AGGGTCATAA ACGCTCTCCA TCTGACGGAA GCACCCCTTA TCAATGTGAA 
CCAGAACCAT CACCTTTCCC TCGCCTCCCT GATCCCCATT TTGTGTTCCC TCCTCCAGTC 
CGACGTAAGG ACACAGGAGT AGAAAGGCCA ACATCTCTAG AATTTGCTCC CAGGCCTCGT 
CCGTCTTCAA ACCGACCTCG AATGGATCCC TGGAAGTTTG TTTCTCTTTC ACAAACTCAT 
AGCTCGTCCC CCTCTAGTGG TGGGGGTGAT GCATGCTCCA GTGGATCAGC AGAGGGAGCT 
CAAGTGGCTG ATGTAGAGGA GACACTGCTT GATATGGAGG TGGAAGGACA AAGACTAGAC 
AGCACAGTAC CTTTGTGCGG ACTGGGTTTA AGACCAACAA CTGACCCTTT TTTTAAATAT 
GGGAACAGAC GTGTCTTAAT GAAAGAATTA AGTATTTCGC TTCTGCAGTA TAAAGTTGAA 
AGTGGGGTGC TTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001082629.1
CDS	97..3114
Translation	MDGLPKDEAFLWQSSKDNKENGVWSDVQSYGVSNPLWMAVFDYEPTAEEELTLRRGDLVEILSKDSTVSGDEGWWTGKIKDKVGIFPSNYVVSDDKYTTLTGAPKQCPLPLEIEFDELNLDEIIGVGGFGKVYKGLWRDEEVAVKAVRHDPDEDINVTAENVRQEAKIFCMLCHPNIIALTGVCLKPPHLCLVMEYARGGPLHRALAGKKVPAHVLVNWAVQIAKGMTYLHNEAIVPIIHRDLGSSNILILEKAENDDLFNKTLNITDFGLAREWQKTTKMSAAGTYAWMAPEVIRLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFVRILEACWDPDPHSRPTFSCILEQLTTIEQSAMFQMPLESFHSLQEDWRLEIQQMFDELRTKEKELRSREEELVRAAEEQRILEDLLKRREQELAEREIDIVERELNIIMYQMYQEKPKVKKRKGNFKKSRLKLKDGNRISLPSGFEHKITVQASPMLDKCKGQGTSSYSPPGSPLIIPRLRAIRLTPVDGSKTWGRSSVLKKEEVTTSNKKKGRTWGPSSTQQKERVGGEERLKTLGEGNKQWSSSAPNLGKSPKHTPISVGFASLTEMEEYADSDGSVPQSPYSQSYLTLPVQSDHRSHPEDTAHAGAPSSDSPKRGSQSRRKSELVLLGCASLLAAVALGSDLSELVPQEEKRKGIFQWAGRGPRRRASSPSRSMSYGEDSVIPSSSVTLISLSSISDCNSTRSLIRSDSDDIGLDHDNVSSGRGVKEDRGQQPNVGSNPLVDYKVESFKRDPKQSLTPTHVTVGRNNTTETRGHRRTPSDGAIRQVTQGHKRSPSDGSTPYQCEPEPSPFPRLPDPHFVFPPPVRRKDTGVERPTSLEFAPRPRPSSNRPRMDPWKFVSLSQTHSSSPSSGGGDACSSGSAEGAQVADVEETLLDMEVEGQRLDSTVPLCGLGLRPTTDPFFKYGNRRVLMKELSISLLQYKVESGVLL

Target ORF information:

RefSeq Version	NM_001089160.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis mitogen-activated protein kinase kinase kinase 10 L homeolog (map3k10.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001089160.1

ORF Insert Sequence:

ATGGATGGAC TTCCGAAAGA TGAGGCCTTT CTGTGGCAGA GCTCAAAAGA CAATAAAGAA 
AATGGGGTGT GGAGTGATGT GCAGAGTTAT GGGGTCTCCA ATCCACTATG GATGGCAGTT 
TTCGATTATG AACCAACAGC AGAAGAGGAA TTAACCTTAC GGAGGGGAGA CTTGGTTGAG 
ATTCTCTCAA AGGACTCTAC TGTTTCTGGT GATGAGGGTT GGTGGACAGG AAAAATTAAA 
GACAAGGTGG GCATATTTCC AAGTAATTAT GTGGTGAGTG ACGATAAATA CACTACCCTA 
ACAGGAGCCC CAAAGCAATG CCCTCTCCCA CTAGAAATCG AGTTTGATGA GCTGAACCTG 
GATGAAATCA TTGGGGTGGG AGGATTTGGG AAGGTCTATA AGGGATTATG GAGAGATGAA 
GAGGTTGCAG TTAAAGCTGT CCGTCATGAC CCAGATGAAG ATATCAACGT GACTGCTGAG 
AATGTGCGAC AGGAAGCCAA GATCTTCTGT ATGTTGTGCC ACCCCAACAT CATAGCCCTG 
ACAGGGGTGT GCCTAAAACC GCCTCATCTT TGTCTGGTTA TGGAGTATGC CAGAGGTGGA 
CCATTACACA GAGCCCTGGC TGGGAAGAAG GTTCCAGCTC ATGTATTGGT AAACTGGGCG 
GTGCAGATAG CCAAAGGCAT GACCTATCTC CACAATGAAG CCATTGTTCC TATTATACAC 
AGGGATCTGG GGTCCAGCAA CATCTTAATA CTAGAGAAAG CAGAGAATGA TGACTTGTTT 
AACAAGACAC TGAATATCAC AGATTTTGGG CTGGCCAGGG AGTGGCAGAA GACAACGAAG 
ATGAGCGCAG CTGGTACCTA TGCTTGGATG GCCCCAGAAG TTATCCGCTT ATCTCTCTTT 
TCTAAGAGCA GTGATGTGTG GAGTTTTGGG GTTCTGCTCT GGGAGCTTCT AACAGGAGAG 
GTGCCATATC GGGAGATAGA TGCTCTTGCT GTAGCATATG GTGTGGCCAT GAACAAGCTT 
ACCTTACCTA TCCCATCGAC TTGTCCTGAG CCTTTTGTAC GGATCCTTGA AGCATGTTGG 
GATCCGGACC CTCACAGCAG ACCTACATTT TCTTGTATTC TGGAACAACT GACAACTATA 
GAACAGTCAG CCATGTTTCA AATGCCCTTG GAGTCCTTCC ATTCTCTTCA GGAAGACTGG 
AGGCTGGAAA TCCAGCAGAT GTTTGATGAA TTGAGGACCA AAGAAAAGGA GCTGCGCAGC 
CGAGAGGAGG AGCTTGTGAG AGCAGCTGAG GAACAGAGGA TTTTGGAGGA TCTGCTGAAG 
AGGAGAGAGC AAGAGTTGGC TGAGAGGGAA ATTGACATTG TTGAGCGAGA GCTGAACATA 
ATTATGTATC AGATGTACCA AGAAAAGCCA AAGGTGAAAA AACGCAAGGG CAATTTCAAG 
AAAAGCCGCC TAAAACTTAA GGATGGCAAC CGGATCAGCT TGCCTTCAGG TTTCGAACAT 
AAGATTACAG TCCAGGCATC TCCAATGTTG GACAAATGTA AGGGGCAGGG GACAAGCAGC 
TATAGCCCAC CTGGGAGTCC ACTAATAATT CCGAGGCTGA GAGCCATTAG ATTAACACCT 
GTTGATGGCA GTAAGACATG GGGGCGCAGT TCTGTACTAA AGAAAGAAGA GGTAACAACA 
TCAAATAAGA AGAAAGGCAG GACTTGGGGA CCTAGCTCGA CACAGCAGAA AGAACGAGTA 
GGGGGAGAAG AAAGGCTGAA GACTCTTGGA GAAGGCAATA AGCAGTGGTC AAGCAGTGCT 
CCCAACCTGG GCAAATCTCC TAAGCATACT CCCATCAGTG TGGGGTTTGC AAGCCTCACT 
GAAATGGAGG AATATGCTGA TTCTGATGGC TCCGTCCCAC AGTCTCCCTA CTCACAGTCC 
TACCTCACAC TGCCTGTCCA ATCAGACCAT AGAAGTCATC CAGAGGACAC AGCGCATGCT 
GGTGCACCCT CATCAGACAG CCCCAAACGT GGTTCGCAGT CCCGTCGGAA AAGTGAACTT 
GTGTTACTGG GGTGTGCGTC TCTCTTAGCT GCTGTTGCTT TGGGCAGTGA CTTATCAGAG 
CTGGTGCCTC AGGAAGAGAA AAGAAAGGGT ATATTCCAAT GGGCAGGGAG AGGTCCCAGG 
CGACGTGCTA GTTCTCCAAG TCGCTCTATG TCCTATGGTG AAGACTCTGT CATTCCATCC 
AGCTCAGTTA CACTCATTTC CTTGTCTTCC ATCTCAGACT GCAATTCCAC AAGGTCTCTG 
ATTCGATCAG ATAGTGATGA CATCGGACTG GACCATGATA ACGTGTCTAG TGGGAGGGGG 
GTAAAAGAGG ACCGAGGACA GCAACCAAAT GTTGGCAGCA ACCCTCTGGT AGATTATAAG 
GTTGAAAGTT TTAAGAGAGA TCCAAAGCAG TCTCTGACTC CCACCCATGT GACAGTAGGA 
AGAAATAACA CAACTGAAAC CAGAGGACAC AGGAGAACCC CTTCCGATGG AGCCATAAGG 
CAAGTGACAC AGGGTCATAA ACGCTCTCCA TCTGACGGAA GCACCCCTTA TCAATGTGAA 
CCAGAACCAT CACCTTTCCC TCGCCTCCCT GATCCCCATT TTGTGTTCCC TCCTCCAGTC 
CGACGTAAGG ACACAGGAGT AGAAAGGCCA ACATCTCTAG AATTTGCTCC CAGGCCTCGT 
CCGTCTTCAA ACCGACCTCG AATGGATCCC TGGAAGTTTG TTTCTCTTTC ACAAACTCAT 
AGCTCGTCCC CCTCTAGTGG TGGGGGTGAT GCATGCTCCA GTGGATCAGC AGAGGGAGCT 
CAAGTGGCTG ATGTAGAGGA GACACTGCTT GATATGGAGG TGGAAGGACA AAGACTAGAC 
AGCACAGTAC CTTTGTGCGG ACTGGGTTTA AGACCAACAA CTGACCCTTT TTTTAAATAT 
GGGAACAGAC GTGTCTTAAT GAAAGAATTA AGTATTTCGC TTCTGCAGTA TAAAGTTGAA 
AGTGGGGTGC TTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PAK interacts with NCK and MLK2 to regulate the activation of jun N-terminal kinase.
FEBS letters543(1-3)129-35(2003 May)
Poitras L,Jean S,Islam N,Moss T

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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map3k10.L cDNA ORF clone, Xenopus laevis(African clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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