cdc25c.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cdc25c.S
Entrez Gene ID	398119
Full Name	cell division cycle 25C L homeolog
Synonyms	cdc25,cdc25-1-b,cdc25C2,cdc25c,cdc25c-a,cdc25c-b,cdc25c.S,xcdc25c
General protein information	Preferred Names cell division cycle 25C L homeolog Names M-phase inducer phosphatase 1-B cell division cycle 25 homolog C cell division cycle 25C S homeolog tyrosine phosphatase
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001088449.1	NP_001081918.1	M-phase inducer phosphatase 1-B
XM_018250797.1	XP_018106286.1	M-phase inducer phosphatase 1-B isoform X2
XM_018250796.1	XP_018106285.1	M-phase inducer phosphatase 1-B isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Direct roles of the signaling kinase RSK2 in Cdc25C activation during Xenopus oocyte maturation.
Wang R, Jung SY, Wu CF, Qin J, Kobayashi R, Gallick GE, Kuang J
Proceedings of the National Academy of Sciences of the United States of America107(46)19885-90(2010 Nov)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Periodic changes in phosphorylation of the Xenopus cdc25 phosphatase regulate its activity.
Izumi T, Walker DH, Maller JL
Molecular biology of the cell3(8)927-39(1992 Aug)

Regulation of the cdc25 protein during the cell cycle in Xenopus extracts.
Kumagai A, Dunphy WG
Cell70(1)139-51(1992 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cdc25c.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cdc25c.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAf23326	XM_018250797.1 Latest version!	Xenopus laevis cell division cycle 25C S homeolog (cdc25c.S), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OAf01006	NM_001088449.1 Latest version!	Xenopus laevis cell division cycle 25C L homeolog (cdc25c.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OAf01006	XM_018250796.1 Latest version!	Xenopus laevis cell division cycle 25C S homeolog (cdc25c.S), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cdc25c.S ( XM_018250797.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018106286.1 Xenopus laevis cell division cycle 25C S homeolog (cdc25c.S), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAf23326
Clone ID Related Accession (Same CDS sequence)	XM_018250797.1
Accession Version	XM_018250797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	M-phase inducer phosphatase 1-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGA GTCACATAAT GTCATCAGAA GCACCCCCAA AAACCAACAC AGGCCTGAAT 
TTCCGTACAA ATTGCCGAAT GGTATTAAAC CTGCTTCGTG AAAAAGATTG TTCTGTTACA 
TTTTCTCCGG AACAGCCTTT GACACCTGTG ACTGACCTTG CTGTTGGATT TAGTAACCTA 
AGTACCTTCA GTGGTGAAAC TCCCAAACGC TGCCTGGACT TGTCCAACCT TGGTGATGAG 
ACTGCACCAC TGCCAACAGA GTCTCCTGAT AGAATATCAT CAGGTAAGGT GGAGTCACCA 
AAGGCACAAT TTGTCCAGTT TGATGGCCTA TTTACTCCTG ATCTCGGGTG GAAAGCAAAA 
AAGTGTCCGA GAGGAAACAT GAATTCTGTT CTGCCACGCC TTCTTTGCAG CACTCCAAGT 
TTTAAAAAGA CCTCAGGAGG ACAGAGAAGT GTGTCTAATA AAGAAAATGA GGGTGAGCTG 
TTTAAGAGTC CAAACTGTAA ACCTGTTGCT TTGCTGCTAC CACAAGAAGT AGTTGATTCA 
CAATTTTCTC CAACACCTGA GAACAAGAAT CCTCCTGGAG CTGTTGGTAA CTTGTCCTCT 
AGCATGGCAA TTCTTCTGTC GGGACCCCTT CTAAATCAAG ACATAGAAGT CAGCAATGTA 
AACAATATAT CTTTAAACAG AAGCCGCCTT TACCGCTCAC CTTCTATGCC AGAGAAACTT 
GACAGGCCAA TGCTTAAACG GCCAGTGAGG CCACTAGACA GTGAAACGCC GGTCAGAGTG 
AAAAGGAGAC GTAGTACCAG CAGCTCCCTT CAACCACAGG AAGAGAACTT CCAACCACAG 
AGGAGGGGTA CTTCTCTTAA AAAGACGCTT TCTCTTTGTG ATGTGGATAT CAGTACAGTT 
TTGGATGAAG ATTGTGGTCA CAGACAGCTA ATTGGAGATT TTACAAAGGT TTATGCTTTA 
CCCACTGTTA CTGGCCGACA TCAGGACCTC CGATACATCA CTGGAGAGAC TTTGGCAGCA 
TTAATTCATG GAGACTTCAG CAGTTTAGTG GAGAAAATCT TTATAATCGA TTGTCGTTAT 
CCTTATGAAT ATGATGGAGG ACACATAAAG GGTGCATTAA ACCTCCACCG GCAAGAGGAG 
GTAACTGACT ACTTCCTGAA ACAACCACTG ACCCCAACGA TGGCACAGAA GCGGTTAATT 
ATCATTTTTC ATTGCGAGTT CTCATCTGAA AGAGGTCCAA AAATGTGTCG GTTCCTCAGG 
GAGGAAGACC GAGCCAGGAA TGAGTACCCA AGCCTCTATT ACCCAGAGCT TTATCTCCTG 
AAAGGTGGTT ACAAGGATTT TTTTCCTGAA TACAAGGAAC TCTGTGAACC GCAGAGCTAC 
TGCCCAATGC ACCACCAAGA CTTCCGGGAG GAGCTGCTGA AATTCCGGAC AAAATGTAAA 
ACTTCGGTTG GTGATCGAAA GAGGAGGGAA CAGATTGCCC GCATAATGAA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018106286.1
CDS	221..1717
Translation	MAESHIMSSEAPPKTNTGLNFRTNCRMVLNLLREKDCSVTFSPEQPLTPVTDLAVGFSNLSTFSGETPKRCLDLSNLGDETAPLPTESPDRISSGKVESPKAQFVQFDGLFTPDLGWKAKKCPRGNMNSVLPRLLCSTPSFKKTSGGQRSVSNKENEGELFKSPNCKPVALLLPQEVVDSQFSPTPENKNPPGAVGNLSSSMAILLSGPLLNQDIEVSNVNNISLNRSRLYRSPSMPEKLDRPMLKRPVRPLDSETPVRVKRRRSTSSSLQPQEENFQPQRRGTSLKKTLSLCDVDISTVLDEDCGHRQLIGDFTKVYALPTVTGRHQDLRYITGETLAALIHGDFSSLVEKIFIIDCRYPYEYDGGHIKGALNLHRQEEVTDYFLKQPLTPTMAQKRLIIIFHCEFSSERGPKMCRFLREEDRARNEYPSLYYPELYLLKGGYKDFFPEYKELCEPQSYCPMHHQDFREELLKFRTKCKTSVGDRKRREQIARIMKL

Target ORF information:

RefSeq Version	XM_018250797.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis cell division cycle 25C S homeolog (cdc25c.S), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018250797.1

ORF Insert Sequence:

ATGGCAGAGA GTCACATAAT GTCATCAGAA GCACCCCCAA AAACCAACAC AGGCCTGAAT 
TTCCGTACAA ATTGCCGAAT GGTATTAAAC CTGCTTCGTG AAAAAGATTG TTCTGTTACA 
TTTTCTCCGG AACAGCCTTT GACACCTGTG ACTGACCTTG CTGTTGGATT TAGTAACCTA 
AGTACCTTCA GTGGTGAAAC TCCCAAACGC TGCCTGGACT TGTCCAACCT TGGTGATGAG 
ACTGCACCAC TGCCAACAGA GTCTCCTGAT AGAATATCAT CAGGTAAGGT GGAGTCACCA 
AAGGCACAAT TTGTCCAGTT TGATGGCCTA TTTACTCCTG ATCTCGGGTG GAAAGCAAAA 
AAGTGTCCGA GAGGAAACAT GAATTCTGTT CTGCCACGCC TTCTTTGCAG CACTCCAAGT 
TTTAAAAAGA CCTCAGGAGG ACAGAGAAGT GTGTCTAATA AAGAAAATGA GGGTGAGCTG 
TTTAAGAGTC CAAACTGTAA ACCTGTTGCT TTGCTGCTAC CACAAGAAGT AGTTGATTCA 
CAATTTTCTC CAACACCTGA GAACAAGAAT CCTCCTGGAG CTGTTGGTAA CTTGTCCTCT 
AGCATGGCAA TTCTTCTGTC GGGACCCCTT CTAAATCAAG ACATAGAAGT CAGCAATGTA 
AACAATATAT CTTTAAACAG AAGCCGCCTT TACCGCTCAC CTTCTATGCC AGAGAAACTT 
GACAGGCCAA TGCTTAAACG GCCAGTGAGG CCACTAGACA GTGAAACGCC GGTCAGAGTG 
AAAAGGAGAC GTAGTACCAG CAGCTCCCTT CAACCACAGG AAGAGAACTT CCAACCACAG 
AGGAGGGGTA CTTCTCTTAA AAAGACGCTT TCTCTTTGTG ATGTGGATAT CAGTACAGTT 
TTGGATGAAG ATTGTGGTCA CAGACAGCTA ATTGGAGATT TTACAAAGGT TTATGCTTTA 
CCCACTGTTA CTGGCCGACA TCAGGACCTC CGATACATCA CTGGAGAGAC TTTGGCAGCA 
TTAATTCATG GAGACTTCAG CAGTTTAGTG GAGAAAATCT TTATAATCGA TTGTCGTTAT 
CCTTATGAAT ATGATGGAGG ACACATAAAG GGTGCATTAA ACCTCCACCG GCAAGAGGAG 
GTAACTGACT ACTTCCTGAA ACAACCACTG ACCCCAACGA TGGCACAGAA GCGGTTAATT 
ATCATTTTTC ATTGCGAGTT CTCATCTGAA AGAGGTCCAA AAATGTGTCG GTTCCTCAGG 
GAGGAAGACC GAGCCAGGAA TGAGTACCCA AGCCTCTATT ACCCAGAGCT TTATCTCCTG 
AAAGGTGGTT ACAAGGATTT TTTTCCTGAA TACAAGGAAC TCTGTGAACC GCAGAGCTAC 
TGCCCAATGC ACCACCAAGA CTTCCGGGAG GAGCTGCTGA AATTCCGGAC AAAATGTAAA 
ACTTCGGTTG GTGATCGAAA GAGGAGGGAA CAGATTGCCC GCATAATGAA GCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc25c.S ( NM_001088449.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001081918.1 Xenopus laevis cell division cycle 25C L homeolog (cdc25c.L), mRNA.

Price & Availability↑

CloneID	OAf01006
Clone ID Related Accession (Same CDS sequence)	NM_001088449.1 , XM_018250796.1
Accession Version	NM_001088449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Xenopus laevis(African clawed frog)
Product	M-phase inducer phosphatase 1-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M96858.1. On Jul 25, 2007 this sequence version replaced NM_001088450.1. ##Evidence-Data-START## Transcript exon combination :: M96858.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00012418, SAMD00012419 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGAGA GTCACATAAT GTCATCAGAA GCACCCCCAA AAACCAACAC AGGCCTGAAT 
TTCCGTACAA ATTGCCGAAT GGTATTAAAC CTGCTTCGTG AAAAAGATTG TTCTGTTACA 
TTTTCTCCGG AACAGCCTTT GACACCTGTG ACTGACCTTG CTGTTGGATT TAGTAACCTA 
AGTACCTTCA GTGGTGAAAC TCCCAAACGC TGCCTGGACT TGTCCAACCT TGGTGATGAG 
ACTGCACCAC TGCCAACAGA GTCTCCTGAT AGAATATCAT CAGGTAAGGT GGAGTCACCA 
AAGGCACAAT TTGTCCAGTT TGATGGCCTA TTTACTCCTG ATCTCGGGTG GAAAGCAAAA 
AAGTGTCCGA GAGGAAACAT GAATTCTGTT CTGCCACGCC TTCTTTGCAG CACTCCAAGT 
TTTAAAAAGA CCTCAGGAGG ACAGAGAAGT GTGTCTAATA AAGAAAATGA GGGTGAGCTG 
TTTAAGAGTC CAAACTGTAA ACCTGTTGCT TTGCTGCTAC CACAAGAAGT AGTTGATTCA 
CAATTTTCTC CAACACCTGA GAACAAGGTG GATATCTCCC TAGATGAGGA TTGTGAGATG 
AATATCTTGG GATCCCCCAT AAGTGCTGAT CCCCCTTGTT TGGATGGCGC ACATGATGAC 
ATCAAAATGC AAAATCTTGA TGGATTTGCA GATTTTTTTT CTGTTGATGA AGAGGAAATG 
GAGAATCCTC CTGGAGCTGT TGGTAACTTG TCCTCTAGCA TGGCAATTCT TCTGTCGGGA 
CCCCTTCTAA ATCAAGACAT AGAAGTCAGC AATGTAAACA ATATATCTTT AAACAGAAGC 
CGCCTTTACC GCTCACCTTC TATGCCAGAG AAACTTGACA GGCCAATGCT TAAACGGCCA 
GTGAGGCCAC TAGACAGTGA AACGCCGGTC AGAGTGAAAA GGAGACGTAG TACCAGCAGC 
TCCCTTCAAC CACAGGAAGA GAACTTCCAA CCACAGAGGA GGGGTACTTC TCTTAAAAAG 
ACGCTTTCTC TTTGTGATGT GGATATCAGT ACAGTTTTGG ATGAAGATTG TGGTCACAGA 
CAGCTAATTG GAGATTTTAC AAAGGTTTAT GCTTTACCCA CTGTTACTGG CCGACATCAG 
GACCTCCGAT ACATCACTGG AGAGACTTTG GCAGCATTAA TTCATGGAGA CTTCAGCAGT 
TTAGTGGAGA AAATCTTTAT AATCGATTGT CGTTATCCTT ATGAATATGA TGGAGGACAC 
ATAAAGGGTG CATTAAACCT CCACCGGCAA GAGGAGGTAA CTGACTACTT CCTGAAACAA 
CCACTGACCC CAACGATGGC ACAGAAGCGG TTAATTATCA TTTTTCATTG CGAGTTCTCA 
TCTGAAAGAG GTCCAAAAAT GTGTCGGTTC CTCAGGGAGG AAGACCGAGC CAGGAATGAG 
TACCCAAGCC TCTATTACCC AGAGCTTTAT CTCCTGAAAG GTGGTTACAA GGATTTTTTT 
CCTGAATACA AGGAACTCTG TGAACCGCAG AGCTACTGCC CAATGCACCA CCAAGACTTC 
CGGGAGGAGC TGCTGAAATT CCGGACAAAA TGTAAAACTT CGGTTGGTGA TCGAAAGAGG 
AGGGAACAGA TTGCCCGCAT AATGAAGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001081918.1
CDS	237..1889
Translation	MAESHIMSSEAPPKTNTGLNFRTNCRMVLNLLREKDCSVTFSPEQPLTPVTDLAVGFSNLSTFSGETPKRCLDLSNLGDETAPLPTESPDRISSGKVESPKAQFVQFDGLFTPDLGWKAKKCPRGNMNSVLPRLLCSTPSFKKTSGGQRSVSNKENEGELFKSPNCKPVALLLPQEVVDSQFSPTPENKVDISLDEDCEMNILGSPISADPPCLDGAHDDIKMQNLDGFADFFSVDEEEMENPPGAVGNLSSSMAILLSGPLLNQDIEVSNVNNISLNRSRLYRSPSMPEKLDRPMLKRPVRPLDSETPVRVKRRRSTSSSLQPQEENFQPQRRGTSLKKTLSLCDVDISTVLDEDCGHRQLIGDFTKVYALPTVTGRHQDLRYITGETLAALIHGDFSSLVEKIFIIDCRYPYEYDGGHIKGALNLHRQEEVTDYFLKQPLTPTMAQKRLIIIFHCEFSSERGPKMCRFLREEDRARNEYPSLYYPELYLLKGGYKDFFPEYKELCEPQSYCPMHHQDFREELLKFRTKCKTSVGDRKRREQIARIMKL

Target ORF information:

RefSeq Version	NM_001088449.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis cell division cycle 25C L homeolog (cdc25c.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001088449.1

ORF Insert Sequence:

ATGGCAGAGA GTCACATAAT GTCATCAGAA GCACCCCCAA AAACCAACAC AGGCCTGAAT 
TTCCGTACAA ATTGCCGAAT GGTATTAAAC CTGCTTCGTG AAAAAGATTG TTCTGTTACA 
TTTTCTCCGG AACAGCCTTT GACACCTGTG ACTGACCTTG CTGTTGGATT TAGTAACCTA 
AGTACCTTCA GTGGTGAAAC TCCCAAACGC TGCCTGGACT TGTCCAACCT TGGTGATGAG 
ACTGCACCAC TGCCAACAGA GTCTCCTGAT AGAATATCAT CAGGTAAGGT GGAGTCACCA 
AAGGCACAAT TTGTCCAGTT TGATGGCCTA TTTACTCCTG ATCTCGGGTG GAAAGCAAAA 
AAGTGTCCGA GAGGAAACAT GAATTCTGTT CTGCCACGCC TTCTTTGCAG CACTCCAAGT 
TTTAAAAAGA CCTCAGGAGG ACAGAGAAGT GTGTCTAATA AAGAAAATGA GGGTGAGCTG 
TTTAAGAGTC CAAACTGTAA ACCTGTTGCT TTGCTGCTAC CACAAGAAGT AGTTGATTCA 
CAATTTTCTC CAACACCTGA GAACAAGGTG GATATCTCCC TAGATGAGGA TTGTGAGATG 
AATATCTTGG GATCCCCCAT AAGTGCTGAT CCCCCTTGTT TGGATGGCGC ACATGATGAC 
ATCAAAATGC AAAATCTTGA TGGATTTGCA GATTTTTTTT CTGTTGATGA AGAGGAAATG 
GAGAATCCTC CTGGAGCTGT TGGTAACTTG TCCTCTAGCA TGGCAATTCT TCTGTCGGGA 
CCCCTTCTAA ATCAAGACAT AGAAGTCAGC AATGTAAACA ATATATCTTT AAACAGAAGC 
CGCCTTTACC GCTCACCTTC TATGCCAGAG AAACTTGACA GGCCAATGCT TAAACGGCCA 
GTGAGGCCAC TAGACAGTGA AACGCCGGTC AGAGTGAAAA GGAGACGTAG TACCAGCAGC 
TCCCTTCAAC CACAGGAAGA GAACTTCCAA CCACAGAGGA GGGGTACTTC TCTTAAAAAG 
ACGCTTTCTC TTTGTGATGT GGATATCAGT ACAGTTTTGG ATGAAGATTG TGGTCACAGA 
CAGCTAATTG GAGATTTTAC AAAGGTTTAT GCTTTACCCA CTGTTACTGG CCGACATCAG 
GACCTCCGAT ACATCACTGG AGAGACTTTG GCAGCATTAA TTCATGGAGA CTTCAGCAGT 
TTAGTGGAGA AAATCTTTAT AATCGATTGT CGTTATCCTT ATGAATATGA TGGAGGACAC 
ATAAAGGGTG CATTAAACCT CCACCGGCAA GAGGAGGTAA CTGACTACTT CCTGAAACAA 
CCACTGACCC CAACGATGGC ACAGAAGCGG TTAATTATCA TTTTTCATTG CGAGTTCTCA 
TCTGAAAGAG GTCCAAAAAT GTGTCGGTTC CTCAGGGAGG AAGACCGAGC CAGGAATGAG 
TACCCAAGCC TCTATTACCC AGAGCTTTAT CTCCTGAAAG GTGGTTACAA GGATTTTTTT 
CCTGAATACA AGGAACTCTG TGAACCGCAG AGCTACTGCC CAATGCACCA CCAAGACTTC 
CGGGAGGAGC TGCTGAAATT CCGGACAAAA TGTAAAACTT CGGTTGGTGA TCGAAAGAGG 
AGGGAACAGA TTGCCCGCAT AATGAAGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cdc25c.S ( XM_018250796.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018106285.1 Xenopus laevis cell division cycle 25C S homeolog (cdc25c.S), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf01006
Clone ID Related Accession (Same CDS sequence)	NM_001088449.1 , XM_018250796.1
Accession Version	XM_018250796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	M-phase inducer phosphatase 1-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGA GTCACATAAT GTCATCAGAA GCACCCCCAA AAACCAACAC AGGCCTGAAT 
TTCCGTACAA ATTGCCGAAT GGTATTAAAC CTGCTTCGTG AAAAAGATTG TTCTGTTACA 
TTTTCTCCGG AACAGCCTTT GACACCTGTG ACTGACCTTG CTGTTGGATT TAGTAACCTA 
AGTACCTTCA GTGGTGAAAC TCCCAAACGC TGCCTGGACT TGTCCAACCT TGGTGATGAG 
ACTGCACCAC TGCCAACAGA GTCTCCTGAT AGAATATCAT CAGGTAAGGT GGAGTCACCA 
AAGGCACAAT TTGTCCAGTT TGATGGCCTA TTTACTCCTG ATCTCGGGTG GAAAGCAAAA 
AAGTGTCCGA GAGGAAACAT GAATTCTGTT CTGCCACGCC TTCTTTGCAG CACTCCAAGT 
TTTAAAAAGA CCTCAGGAGG ACAGAGAAGT GTGTCTAATA AAGAAAATGA GGGTGAGCTG 
TTTAAGAGTC CAAACTGTAA ACCTGTTGCT TTGCTGCTAC CACAAGAAGT AGTTGATTCA 
CAATTTTCTC CAACACCTGA GAACAAGGTG GATATCTCCC TAGATGAGGA TTGTGAGATG 
AATATCTTGG GATCCCCCAT AAGTGCTGAT CCCCCTTGTT TGGATGGCGC ACATGATGAC 
ATCAAAATGC AAAATCTTGA TGGATTTGCA GATTTTTTTT CTGTTGATGA AGAGGAAATG 
GAGAATCCTC CTGGAGCTGT TGGTAACTTG TCCTCTAGCA TGGCAATTCT TCTGTCGGGA 
CCCCTTCTAA ATCAAGACAT AGAAGTCAGC AATGTAAACA ATATATCTTT AAACAGAAGC 
CGCCTTTACC GCTCACCTTC TATGCCAGAG AAACTTGACA GGCCAATGCT TAAACGGCCA 
GTGAGGCCAC TAGACAGTGA AACGCCGGTC AGAGTGAAAA GGAGACGTAG TACCAGCAGC 
TCCCTTCAAC CACAGGAAGA GAACTTCCAA CCACAGAGGA GGGGTACTTC TCTTAAAAAG 
ACGCTTTCTC TTTGTGATGT GGATATCAGT ACAGTTTTGG ATGAAGATTG TGGTCACAGA 
CAGCTAATTG GAGATTTTAC AAAGGTTTAT GCTTTACCCA CTGTTACTGG CCGACATCAG 
GACCTCCGAT ACATCACTGG AGAGACTTTG GCAGCATTAA TTCATGGAGA CTTCAGCAGT 
TTAGTGGAGA AAATCTTTAT AATCGATTGT CGTTATCCTT ATGAATATGA TGGAGGACAC 
ATAAAGGGTG CATTAAACCT CCACCGGCAA GAGGAGGTAA CTGACTACTT CCTGAAACAA 
CCACTGACCC CAACGATGGC ACAGAAGCGG TTAATTATCA TTTTTCATTG CGAGTTCTCA 
TCTGAAAGAG GTCCAAAAAT GTGTCGGTTC CTCAGGGAGG AAGACCGAGC CAGGAATGAG 
TACCCAAGCC TCTATTACCC AGAGCTTTAT CTCCTGAAAG GTGGTTACAA GGATTTTTTT 
CCTGAATACA AGGAACTCTG TGAACCGCAG AGCTACTGCC CAATGCACCA CCAAGACTTC 
CGGGAGGAGC TGCTGAAATT CCGGACAAAA TGTAAAACTT CGGTTGGTGA TCGAAAGAGG 
AGGGAACAGA TTGCCCGCAT AATGAAGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018106285.1
CDS	124..1776
Translation	MAESHIMSSEAPPKTNTGLNFRTNCRMVLNLLREKDCSVTFSPEQPLTPVTDLAVGFSNLSTFSGETPKRCLDLSNLGDETAPLPTESPDRISSGKVESPKAQFVQFDGLFTPDLGWKAKKCPRGNMNSVLPRLLCSTPSFKKTSGGQRSVSNKENEGELFKSPNCKPVALLLPQEVVDSQFSPTPENKVDISLDEDCEMNILGSPISADPPCLDGAHDDIKMQNLDGFADFFSVDEEEMENPPGAVGNLSSSMAILLSGPLLNQDIEVSNVNNISLNRSRLYRSPSMPEKLDRPMLKRPVRPLDSETPVRVKRRRSTSSSLQPQEENFQPQRRGTSLKKTLSLCDVDISTVLDEDCGHRQLIGDFTKVYALPTVTGRHQDLRYITGETLAALIHGDFSSLVEKIFIIDCRYPYEYDGGHIKGALNLHRQEEVTDYFLKQPLTPTMAQKRLIIIFHCEFSSERGPKMCRFLREEDRARNEYPSLYYPELYLLKGGYKDFFPEYKELCEPQSYCPMHHQDFREELLKFRTKCKTSVGDRKRREQIARIMKL

Target ORF information:

RefSeq Version	XM_018250796.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis cell division cycle 25C S homeolog (cdc25c.S), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018250796.1

ORF Insert Sequence:

ATGGCAGAGA GTCACATAAT GTCATCAGAA GCACCCCCAA AAACCAACAC AGGCCTGAAT 
TTCCGTACAA ATTGCCGAAT GGTATTAAAC CTGCTTCGTG AAAAAGATTG TTCTGTTACA 
TTTTCTCCGG AACAGCCTTT GACACCTGTG ACTGACCTTG CTGTTGGATT TAGTAACCTA 
AGTACCTTCA GTGGTGAAAC TCCCAAACGC TGCCTGGACT TGTCCAACCT TGGTGATGAG 
ACTGCACCAC TGCCAACAGA GTCTCCTGAT AGAATATCAT CAGGTAAGGT GGAGTCACCA 
AAGGCACAAT TTGTCCAGTT TGATGGCCTA TTTACTCCTG ATCTCGGGTG GAAAGCAAAA 
AAGTGTCCGA GAGGAAACAT GAATTCTGTT CTGCCACGCC TTCTTTGCAG CACTCCAAGT 
TTTAAAAAGA CCTCAGGAGG ACAGAGAAGT GTGTCTAATA AAGAAAATGA GGGTGAGCTG 
TTTAAGAGTC CAAACTGTAA ACCTGTTGCT TTGCTGCTAC CACAAGAAGT AGTTGATTCA 
CAATTTTCTC CAACACCTGA GAACAAGGTG GATATCTCCC TAGATGAGGA TTGTGAGATG 
AATATCTTGG GATCCCCCAT AAGTGCTGAT CCCCCTTGTT TGGATGGCGC ACATGATGAC 
ATCAAAATGC AAAATCTTGA TGGATTTGCA GATTTTTTTT CTGTTGATGA AGAGGAAATG 
GAGAATCCTC CTGGAGCTGT TGGTAACTTG TCCTCTAGCA TGGCAATTCT TCTGTCGGGA 
CCCCTTCTAA ATCAAGACAT AGAAGTCAGC AATGTAAACA ATATATCTTT AAACAGAAGC 
CGCCTTTACC GCTCACCTTC TATGCCAGAG AAACTTGACA GGCCAATGCT TAAACGGCCA 
GTGAGGCCAC TAGACAGTGA AACGCCGGTC AGAGTGAAAA GGAGACGTAG TACCAGCAGC 
TCCCTTCAAC CACAGGAAGA GAACTTCCAA CCACAGAGGA GGGGTACTTC TCTTAAAAAG 
ACGCTTTCTC TTTGTGATGT GGATATCAGT ACAGTTTTGG ATGAAGATTG TGGTCACAGA 
CAGCTAATTG GAGATTTTAC AAAGGTTTAT GCTTTACCCA CTGTTACTGG CCGACATCAG 
GACCTCCGAT ACATCACTGG AGAGACTTTG GCAGCATTAA TTCATGGAGA CTTCAGCAGT 
TTAGTGGAGA AAATCTTTAT AATCGATTGT CGTTATCCTT ATGAATATGA TGGAGGACAC 
ATAAAGGGTG CATTAAACCT CCACCGGCAA GAGGAGGTAA CTGACTACTT CCTGAAACAA 
CCACTGACCC CAACGATGGC ACAGAAGCGG TTAATTATCA TTTTTCATTG CGAGTTCTCA 
TCTGAAAGAG GTCCAAAAAT GTGTCGGTTC CTCAGGGAGG AAGACCGAGC CAGGAATGAG 
TACCCAAGCC TCTATTACCC AGAGCTTTAT CTCCTGAAAG GTGGTTACAA GGATTTTTTT 
CCTGAATACA AGGAACTCTG TGAACCGCAG AGCTACTGCC CAATGCACCA CCAAGACTTC 
CGGGAGGAGC TGCTGAAATT CCGGACAAAA TGTAAAACTT CGGTTGGTGA TCGAAAGAGG 
AGGGAACAGA TTGCCCGCAT AATGAAGCTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Direct roles of the signaling kinase RSK2 in Cdc25C activation during Xenopus oocyte maturation.
Proceedings of the National Academy of Sciences of the United States of America107(46)19885-90(2010 Nov)
Wang R,Jung SY,Wu CF,Qin J,Kobayashi R,Gallick GE,Kuang J

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Periodic changes in phosphorylation of the Xenopus cdc25 phosphatase regulate its activity.
Molecular biology of the cell3(8)927-39(1992 Aug)
Izumi T,Walker DH,Maller JL

Regulation of the cdc25 protein during the cell cycle in Xenopus extracts.
Cell70(1)139-51(1992 Jul)
Kumagai A,Dunphy WG

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cdc25c.S cDNA ORF clone, Xenopus laevis(African clawed frog)

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