LOC108716825 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108716825
Entrez Gene ID	108716825
Full Name	laminin subunit alpha 4 L homeolog
General protein information	Preferred Names laminin subunit alpha 4 L homeolog Names laminin subunit alpha-4-like
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018263293.1	XP_018118782.1	laminin subunit alpha-4-like isoform X2
XM_018263292.1	XP_018118781.1	laminin subunit alpha-4-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC108716825 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108716825 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf32488	XM_018263292.1 Latest version!	Xenopus laevis laminin subunit alpha-4-like (LOC108716825), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAf32489	XM_018263293.1 Latest version!	Xenopus laevis laminin subunit alpha-4-like (LOC108716825), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108716825 ( XM_018263292.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018118781.1 Xenopus laevis laminin subunit alpha-4-like (LOC108716825), transcript variant X1, mRNA.

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CloneID	OAf32488
Clone ID Related Accession (Same CDS sequence)	XM_018263292.1
Accession Version	XM_018263292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	laminin subunit alpha-4-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030732.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCACT TTTCCCGAGG GTATCTTATA TGCCTGTTAA TGTCACTATG GAGGTATCTG 
TGCCTCTGCA GTGGCTCAAT GGAAGGCAGC GCTTTCCATT TGGACTCAGA AGGCAGCACT 
TCCAACCCCA GCGGCCAGGA CTCGGGCATG GTACAAGTGC AGCACGTTTT CATCCCTGCC 
AAGCTCGTAC CACTGGCACA GCGATGTCAT TTGGGATTTT ATTATTCAGT TTCCGGAGAA 
TGTTTACCCT GTAATTGCAA TGGAAACTCA CAGAAGTGCT TGGATGGATC TGGAGAATGT 
CTGGATTGTC AAAGGAATAC AACAGGGGAA CACTGTGAAA GATGCCCACC AGGTTACCTA 
GGAAATGTGG TCCGAGGAGT ACCCAAATCT TGTGTGCCAT GTCCTTGCCC TCTACCATTT 
AGTCCTAACA ATTTTGCTCT TGCTTGCGGT GTCAAGAGTG GTTCTATGCG CTGTATCTGC 
AAGGACAATT ATGCTGGGCC AAACTGTGAA AGGTGTGCCC CAGGCTATTA TGGAAATCCT 
CTTCTAAAGG GAAGTATATG TAAAAAATGT GACTGCAGTG GGAACTCAGA TCCTAACCTG 
ATATTTGAAG ACTGCAATGA GTTGACAGGA CAATGCAATA ATTGTATACA CAACACTACT 
GGTTTTAACT GTGAACAATG TGCACCGGGG TATTATGGCG ATGCTAGAGT GGCTAAAAAC 
TGCAAAGAGT GCCGCTGTTC AAAATGTGGT ACAGAAACAT GCAATGATGT TACCGGGGCA 
TGTCAATGCA AACCAGGAGT CACTGGTGTT TTCTGTGATC GTTGTGAAAC AGGTCACTAT 
GGTTATAGCA GTTGCTTGGG CTGTCAGAAA TGTATGTGTA GCCTGGCTTC TCTGGATAAC 
AACTGTGACC CCGAAACACG ACAGTGTAAT TGTAAGCCTG GTGCAGGCGG TCTGAAATGT 
GAGAGATGCA AGCCAGGATA CTGGAATTAT GGACCATCTG GATGTCAGAA ATGTGACTGT 
GGAGGTGGCC CATGTAACAA TAAAACTGGG GAATGTTTAT TAGAGGACCC AGAATCTCCA 
GCAAATTCCG ACTGTTCATT TGATTGTGAT AAGTGTATAT GGGACTTAAT TGATGACCTC 
AGAATAGCTT CATCACTAGC AGATGAAACA AAGACTACAG CGCTTAGTAT ATCAACTGGT 
GTTGCGGCTC ATAAACATCT AAATTACATC AATTCCACAC TGATCCAGCT TACGGAAATG 
CTGGAGGAGA AGAACAATCA ATCTGTATTT ACAGATCTCA TGATTGAAAA TGCAGAAATT 
AAAGCAGAAG ATCTTCAGAC CAAAGTAAAT AAGCTTGCAG ACAAAGGAAA TCTAGCTGAG 
ATGAAAGGAT CTCGATTTCA CAAAGAAACC ATGGAAACCA TGAAACGGGC TAAGTTGACT 
GCAGGACAAG TAAATGATAT AGAAGATGGC ATCCAAGAAC TACTTGGCAA ATTAGAATAT 
TATGAATCAT TGCAAGGAGA CCTGAGCACC AGTGATCGTG TTAAAAGTAA GAAACAAGCA 
GAGCAAATGC TTGAGGATAT GGAAGAACTG AACTTCAGTA CACAAAGGAT TACCCTAGAA 
GAAGAAAAGG AATATGTTGA AGAGTTGCTG ACTGAAATCT TAGATGATTG GCAGCAAGTA 
CAAAACAATA CCCGATCTTT TCTTCCTAAC GTACTAGAGA AAATTGCAGA ACACGATGAT 
GAGCTTGCAG ATCTGTATGA AGCAATGGAG GAAGCACGCC AACACATCAA CCAAACTACA 
GAAAAAAATA AGGCAAACAC TGGCAAGCTT CAAATGTCCA ATATTCAAAC AGTAAAGGTG 
ATTAAGGAAG TGGAAAATGT CAGTGGAACA TTGACACAGG CTAACATAAC ATTGAATGGC 
GCAAACATGC TTATTTCTGA CATCAGCGAT ATCACCAAGA ACATGTCTGG ACATCATGCT 
GAAATAGATG GAGCTTACAT CGGACTAAAA GAAAGACTTG CAAATTTGTC TCATAATGTT 
GAAAATATAG TTGAAGAAGC TGTGACTCAC TCTCTTAACC TTCAAAGAGA AGCAAATGGG 
CTGACCAGCG ACTTAAAGGG AATCGATGCA AATGGATTTG TTCAAAAAGC CATTGATGCC 
TCCAATGTCC ATGAGAGCAT TGTTAATAGG ATTGAAGCTG CTAATGAGAC GTCATTTATT 
GCACTAAATA CAGCAGAAAG AGTTAATGAT GCTTCAGATG GAATGGACAA CCAGATAAAG 
TATCAGATGA CAGAGAATGA GAAACTGTTG GTTCAAGCTA AAGAACTGAA GGAGGCATCT 
GATTCGAGAA AAGACTTAGG AATTTCAGAA ACGAAACAAC GTGTAAATGA TGCTGCTCTG 
AGAAAAGATG CTTTAAGCGA TAGACTAGAA AATGCCATTT CTCAGATTGA AGTACACGAA 
GATGAGAACA CAAGAAAGCG ACTAGAAAAA TCGAAACTGG TTGCTGAGGA AGCCTTAAAC 
ATCACAGCTA TGATATCAAA ATTGACCAAT CCAATGTCCA AGAATGCCAA GATTTGGACC 
AAAGATCTTA TTGATACAGA TTTTGATGCT TCTGCTTATA ACAAGGTTGT AAACTCAGCA 
GGAGAGGCTG TTAGGAACCT CACAGAGGTA GTCCCTTTGC TTCTGAATAA GCTGCGAACA 
GTAGAATACA AGTGGCCTAC AAACAATATA TCATCAAGCA TTCTCCGCAT TAGGGAGCTT 
ATAGCACAAA CAAGGAGTGT GGCCAGTAAG GTCCAAGTGT CAATGCGGTT TGGAGGTAAA 
TCAGCTGTTG ATGTGAGTCT AAGAACAAAT GTGGCAGACC TGAAAGCATA TTCTTCTATC 
AGTTTGTACC TAAACCCGAT TGCACACCAG GAAAAACCTC ATGACAAACT CATTATGTAT 
TTCGGAAATA AGAATTCTAC ATCAGACTAC ATGGGTCTTG CAATTAAGAG CAACAATCTG 
GTTTATGTGT ACAATCTGGG AAGTGGAGAT GTTGAGATTC CACTGGATTC CAAGCCTGTC 
AACTCCTGGC CCGGATATTT TAGTCTCATC AAAATTGAAA GGCTGGGCCG ACATGGCAAA 
GTGCTTTTAA CTGTACCTAG TCCAAGTAGC ACAGCAGAAG AAAAATTCAT TAAGAAGGGT 
GAGGCTACTG GAAGAGATTC TGTTCTAGAT CTAGATCCCG CCAGCATGGT GTTTTATGTT 
GGTGGGGTTC CTTCAGGATT TCAGCTTCCC GCCAGCTTAA ATCTGCCAGG CTTTGTTGGT 
TGTCTAGAGC TTGCTACTGT CAATGATGAT GTTGTTAGCT TGTACAATTT TAAGAACATC 
TACAACATGA ACACAGTCAC AGCACCACCG TGTGCCAGGA ATAAATTGGC CTTCACACAG 
AGCCGTGCAG CAAGCTATTT CTTTGATGGG ACTGGATATG CTGTTATACG CAATATAGAA 
AGGAGGGGTC GCTTTACCCA AGTCACTCGC TTTGATATAG AAGTCCGAAC ACCAGTAGAT 
AATGCCCTTA TTTTACTCAT GGTTAATGGA ACCAAATTCT TTAGTCTGGA GCTCCAAGAT 
GGATATCTGA GACTGCAGTA TGATTTTGGC TTTGCCAAGG GGCCTGTGCT TCTTGAAGAT 
TCAGCGAAGA AGTTTCAGAT AAACGATGCA AGATATCATG AGATCTCAGT CATTTATCAC 
AATTCCAAGA AGATGATATT GGTGGTTGAC AGAAGACATG TGAAGAGTGT TGACAATGAA 
AAGACATCAA TTCCTTTCTC TGACGTTTAT ATTGGAGGAG CGCCTTCTCC AGTTTTGCAG 
AGTGTGAAGA GTCACCTAGC TGCAGACATT TCCTATAAGG GATGCATAAA AGGTTTCCAG 
TTTCAGAAGA AGGATTTCAA TTTGCTGGAA GAACCAGAGA CGCTTGGTAT CAGCTATGGC 
TGTCCTGAAG AATCACTTAT GTCCCGTCGA GCTTATTTTA ATGGGCAAAG TTTTATTGCT 
TCTAGTCAGA AGCTGGCTCC TTTCGAGTCC TTTGAAGGTG GCTTTAGTTT CAAAACTTTA 
CAGCCCAGCG GTCTGTTGCT TTATCACTCT GAAGGGGCTG ATATGTTTTC ATTATCCATG 
GATAAAGGAT CAGTTGTCTT CAGAATAAAA GACATCAAAG TGCAATCTAA AAATAGGAAA 
TACAATGATG GGAAGAACCA CTTTGTGGTT GCTTCAGTCT CCCCAACAAG GTATCAGTTG 
CTGGTAGATG AAACAGATGC AAGTGTAGAA GATAGACAGA AATCTGATGC AAGCTCTCCG 
GTGGCAAGAA AGTTTTATTT TGGAGGCACC CCAACTGGAG TTTCCCAGGC CAATTTCACA 
GGGTGTATCA GCAATGCTTA TTTCACAAGA GTTGACAGAG ATGTTGAAGT TGAGGATTTT 
CAGAAATACA CAGAGAAAGT CCAACCTTCT CTTTATGGGT GTCCAGTTGA AACACCTCCT 
ATTGCTCTGC ATCAAAAGCA TGGTAAAAAT TCCTCTAAAG ACAAAGGAAA GCTAAAGAAG 
AGTGTTGGAA TGGAAAAAAC ATTAGAGTCT CAAGCTGCAA TTGGGTCCAA AGAGACAGAA 
CAAGACTTAG AATTAGTCTC GCAGTGCACC CTCTCTAGCA GACCAAAGGC TGCCAGACAG 
GCACATCTCT ATGGTGGCAT AGCCAACAGC CGACAGGAAT TCAAACAGAT CCCTAATGGC 
TTCAATGAAA AATCTGAACT GTTTGTAAGA CTGAAGACAT ACTCATCCAA TGGAATGATC 
TTCTATGTCT CTGATAAAGA GGAAAACAAT TTTATGACTC TCTTTCTGTC TCATGGCCGA 
CTCACCTTCA TGTTTAACCA TGGGCAGCAA AAGATAAGGA TCAAGTCTCA AGAGAAGTAC 
AACGATGGCT TATGGCACAG TGTGTTATGC ATCCGGGAAA AGAACAAAGG AAGATTAATC 
ATTAATGGGC TCCGTGTATT AGAAGACAGT ACTTCTGTTG CTGCTGTTAT TTGGACTGTG 
GATGCACCAT TTTATCTAGG AGGTGTAGCG CCTGGAATAG CAATCAAAAA TGTTCAGATC 
AACTCAGCGT ACAGTTTCAG TGGCTGCCTT AGCAATTTGC AGATGAATGG ACGCTCTTTG 
ACGTCACCTT CCCAGACATT CAGTGTAACA CCTTGTTTTG AAGGCCCCAT GGAATCTGGA 
ACCTACATTT CATCAGAAGG AGGATATGTG ATTCTTGATG ACTCCTTCAG TACTGGGTTA 
ACATTTGAGT TTGCCCTTGA AGTCCGTCCA CGAAATAACT CTGGAATCTT GATTCACATA 
CAAAATGTGA ATGGAGAGTA TTTGAATTTA CACATGAAGC AGGGACAGGT TGTTGTTAAG 
GTGAAAAATG GCAACAGAGA GTTTTCTACA CATGTCCTAC CAAAACAGAG TCTCTGTGAT 
GGCCAGTGGC ACCGAATTGC AGTGATACGA GATGGAAATG TAGTACAACT GGATGTGGAT 
TCTGAGATAA ACCATGTGGT GGGCCCCATT AACACCAAAG TGCTGGAGCT GAAAGAGCCT 
GTGTTTGTTG GAGGTGTACC AAATTCTTTA CTTGGGCCTA CACTGACTAC ACGCAACTCT 
TTTACTGGCT GTATCCGTAA CTTTGTGATA GATGAACATG CTGTAAGTTT CAATAAAGCA 
TCTCTTGTCA GTGGGGCAGT GAGCATCAAT TCCTGCCCTG CCGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018118781.1
CDS	365..6112
Translation	MGHFSRGYLICLLMSLWRYLCLCSGSMEGSAFHLDSEGSTSNPSGQDSGMVQVQHVFIPAKLVPLAQRCHLGFYYSVSGECLPCNCNGNSQKCLDGSGECLDCQRNTTGEHCERCPPGYLGNVVRGVPKSCVPCPCPLPFSPNNFALACGVKSGSMRCICKDNYAGPNCERCAPGYYGNPLLKGSICKKCDCSGNSDPNLIFEDCNELTGQCNNCIHNTTGFNCEQCAPGYYGDARVAKNCKECRCSKCGTETCNDVTGACQCKPGVTGVFCDRCETGHYGYSSCLGCQKCMCSLASLDNNCDPETRQCNCKPGAGGLKCERCKPGYWNYGPSGCQKCDCGGGPCNNKTGECLLEDPESPANSDCSFDCDKCIWDLIDDLRIASSLADETKTTALSISTGVAAHKHLNYINSTLIQLTEMLEEKNNQSVFTDLMIENAEIKAEDLQTKVNKLADKGNLAEMKGSRFHKETMETMKRAKLTAGQVNDIEDGIQELLGKLEYYESLQGDLSTSDRVKSKKQAEQMLEDMEELNFSTQRITLEEEKEYVEELLTEILDDWQQVQNNTRSFLPNVLEKIAEHDDELADLYEAMEEARQHINQTTEKNKANTGKLQMSNIQTVKVIKEVENVSGTLTQANITLNGANMLISDISDITKNMSGHHAEIDGAYIGLKERLANLSHNVENIVEEAVTHSLNLQREANGLTSDLKGIDANGFVQKAIDASNVHESIVNRIEAANETSFIALNTAERVNDASDGMDNQIKYQMTENEKLLVQAKELKEASDSRKDLGISETKQRVNDAALRKDALSDRLENAISQIEVHEDENTRKRLEKSKLVAEEALNITAMISKLTNPMSKNAKIWTKDLIDTDFDASAYNKVVNSAGEAVRNLTEVVPLLLNKLRTVEYKWPTNNISSSILRIRELIAQTRSVASKVQVSMRFGGKSAVDVSLRTNVADLKAYSSISLYLNPIAHQEKPHDKLIMYFGNKNSTSDYMGLAIKSNNLVYVYNLGSGDVEIPLDSKPVNSWPGYFSLIKIERLGRHGKVLLTVPSPSSTAEEKFIKKGEATGRDSVLDLDPASMVFYVGGVPSGFQLPASLNLPGFVGCLELATVNDDVVSLYNFKNIYNMNTVTAPPCARNKLAFTQSRAASYFFDGTGYAVIRNIERRGRFTQVTRFDIEVRTPVDNALILLMVNGTKFFSLELQDGYLRLQYDFGFAKGPVLLEDSAKKFQINDARYHEISVIYHNSKKMILVVDRRHVKSVDNEKTSIPFSDVYIGGAPSPVLQSVKSHLAADISYKGCIKGFQFQKKDFNLLEEPETLGISYGCPEESLMSRRAYFNGQSFIASSQKLAPFESFEGGFSFKTLQPSGLLLYHSEGADMFSLSMDKGSVVFRIKDIKVQSKNRKYNDGKNHFVVASVSPTRYQLLVDETDASVEDRQKSDASSPVARKFYFGGTPTGVSQANFTGCISNAYFTRVDRDVEVEDFQKYTEKVQPSLYGCPVETPPIALHQKHGKNSSKDKGKLKKSVGMEKTLESQAAIGSKETEQDLELVSQCTLSSRPKAARQAHLYGGIANSRQEFKQIPNGFNEKSELFVRLKTYSSNGMIFYVSDKEENNFMTLFLSHGRLTFMFNHGQQKIRIKSQEKYNDGLWHSVLCIREKNKGRLIINGLRVLEDSTSVAAVIWTVDAPFYLGGVAPGIAIKNVQINSAYSFSGCLSNLQMNGRSLTSPSQTFSVTPCFEGPMESGTYISSEGGYVILDDSFSTGLTFEFALEVRPRNNSGILIHIQNVNGEYLNLHMKQGQVVVKVKNGNREFSTHVLPKQSLCDGQWHRIAVIRDGNVVQLDVDSEINHVVGPINTKVLELKEPVFVGGVPNSLLGPTLTTRNSFTGCIRNFVIDEHAVSFNKASLVSGAVSINSCPAA

Target ORF information:

RefSeq Version	XM_018263292.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis laminin subunit alpha-4-like (LOC108716825), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018263292.1

ORF Insert Sequence:

ATGGGTCACT TTTCCCGAGG GTATCTTATA TGCCTGTTAA TGTCACTATG GAGGTATCTG 
TGCCTCTGCA GTGGCTCAAT GGAAGGCAGC GCTTTCCATT TGGACTCAGA AGGCAGCACT 
TCCAACCCCA GCGGCCAGGA CTCGGGCATG GTACAAGTGC AGCACGTTTT CATCCCTGCC 
AAGCTCGTAC CACTGGCACA GCGATGTCAT TTGGGATTTT ATTATTCAGT TTCCGGAGAA 
TGTTTACCCT GTAATTGCAA TGGAAACTCA CAGAAGTGCT TGGATGGATC TGGAGAATGT 
CTGGATTGTC AAAGGAATAC AACAGGGGAA CACTGTGAAA GATGCCCACC AGGTTACCTA 
GGAAATGTGG TCCGAGGAGT ACCCAAATCT TGTGTGCCAT GTCCTTGCCC TCTACCATTT 
AGTCCTAACA ATTTTGCTCT TGCTTGCGGT GTCAAGAGTG GTTCTATGCG CTGTATCTGC 
AAGGACAATT ATGCTGGGCC AAACTGTGAA AGGTGTGCCC CAGGCTATTA TGGAAATCCT 
CTTCTAAAGG GAAGTATATG TAAAAAATGT GACTGCAGTG GGAACTCAGA TCCTAACCTG 
ATATTTGAAG ACTGCAATGA GTTGACAGGA CAATGCAATA ATTGTATACA CAACACTACT 
GGTTTTAACT GTGAACAATG TGCACCGGGG TATTATGGCG ATGCTAGAGT GGCTAAAAAC 
TGCAAAGAGT GCCGCTGTTC AAAATGTGGT ACAGAAACAT GCAATGATGT TACCGGGGCA 
TGTCAATGCA AACCAGGAGT CACTGGTGTT TTCTGTGATC GTTGTGAAAC AGGTCACTAT 
GGTTATAGCA GTTGCTTGGG CTGTCAGAAA TGTATGTGTA GCCTGGCTTC TCTGGATAAC 
AACTGTGACC CCGAAACACG ACAGTGTAAT TGTAAGCCTG GTGCAGGCGG TCTGAAATGT 
GAGAGATGCA AGCCAGGATA CTGGAATTAT GGACCATCTG GATGTCAGAA ATGTGACTGT 
GGAGGTGGCC CATGTAACAA TAAAACTGGG GAATGTTTAT TAGAGGACCC AGAATCTCCA 
GCAAATTCCG ACTGTTCATT TGATTGTGAT AAGTGTATAT GGGACTTAAT TGATGACCTC 
AGAATAGCTT CATCACTAGC AGATGAAACA AAGACTACAG CGCTTAGTAT ATCAACTGGT 
GTTGCGGCTC ATAAACATCT AAATTACATC AATTCCACAC TGATCCAGCT TACGGAAATG 
CTGGAGGAGA AGAACAATCA ATCTGTATTT ACAGATCTCA TGATTGAAAA TGCAGAAATT 
AAAGCAGAAG ATCTTCAGAC CAAAGTAAAT AAGCTTGCAG ACAAAGGAAA TCTAGCTGAG 
ATGAAAGGAT CTCGATTTCA CAAAGAAACC ATGGAAACCA TGAAACGGGC TAAGTTGACT 
GCAGGACAAG TAAATGATAT AGAAGATGGC ATCCAAGAAC TACTTGGCAA ATTAGAATAT 
TATGAATCAT TGCAAGGAGA CCTGAGCACC AGTGATCGTG TTAAAAGTAA GAAACAAGCA 
GAGCAAATGC TTGAGGATAT GGAAGAACTG AACTTCAGTA CACAAAGGAT TACCCTAGAA 
GAAGAAAAGG AATATGTTGA AGAGTTGCTG ACTGAAATCT TAGATGATTG GCAGCAAGTA 
CAAAACAATA CCCGATCTTT TCTTCCTAAC GTACTAGAGA AAATTGCAGA ACACGATGAT 
GAGCTTGCAG ATCTGTATGA AGCAATGGAG GAAGCACGCC AACACATCAA CCAAACTACA 
GAAAAAAATA AGGCAAACAC TGGCAAGCTT CAAATGTCCA ATATTCAAAC AGTAAAGGTG 
ATTAAGGAAG TGGAAAATGT CAGTGGAACA TTGACACAGG CTAACATAAC ATTGAATGGC 
GCAAACATGC TTATTTCTGA CATCAGCGAT ATCACCAAGA ACATGTCTGG ACATCATGCT 
GAAATAGATG GAGCTTACAT CGGACTAAAA GAAAGACTTG CAAATTTGTC TCATAATGTT 
GAAAATATAG TTGAAGAAGC TGTGACTCAC TCTCTTAACC TTCAAAGAGA AGCAAATGGG 
CTGACCAGCG ACTTAAAGGG AATCGATGCA AATGGATTTG TTCAAAAAGC CATTGATGCC 
TCCAATGTCC ATGAGAGCAT TGTTAATAGG ATTGAAGCTG CTAATGAGAC GTCATTTATT 
GCACTAAATA CAGCAGAAAG AGTTAATGAT GCTTCAGATG GAATGGACAA CCAGATAAAG 
TATCAGATGA CAGAGAATGA GAAACTGTTG GTTCAAGCTA AAGAACTGAA GGAGGCATCT 
GATTCGAGAA AAGACTTAGG AATTTCAGAA ACGAAACAAC GTGTAAATGA TGCTGCTCTG 
AGAAAAGATG CTTTAAGCGA TAGACTAGAA AATGCCATTT CTCAGATTGA AGTACACGAA 
GATGAGAACA CAAGAAAGCG ACTAGAAAAA TCGAAACTGG TTGCTGAGGA AGCCTTAAAC 
ATCACAGCTA TGATATCAAA ATTGACCAAT CCAATGTCCA AGAATGCCAA GATTTGGACC 
AAAGATCTTA TTGATACAGA TTTTGATGCT TCTGCTTATA ACAAGGTTGT AAACTCAGCA 
GGAGAGGCTG TTAGGAACCT CACAGAGGTA GTCCCTTTGC TTCTGAATAA GCTGCGAACA 
GTAGAATACA AGTGGCCTAC AAACAATATA TCATCAAGCA TTCTCCGCAT TAGGGAGCTT 
ATAGCACAAA CAAGGAGTGT GGCCAGTAAG GTCCAAGTGT CAATGCGGTT TGGAGGTAAA 
TCAGCTGTTG ATGTGAGTCT AAGAACAAAT GTGGCAGACC TGAAAGCATA TTCTTCTATC 
AGTTTGTACC TAAACCCGAT TGCACACCAG GAAAAACCTC ATGACAAACT CATTATGTAT 
TTCGGAAATA AGAATTCTAC ATCAGACTAC ATGGGTCTTG CAATTAAGAG CAACAATCTG 
GTTTATGTGT ACAATCTGGG AAGTGGAGAT GTTGAGATTC CACTGGATTC CAAGCCTGTC 
AACTCCTGGC CCGGATATTT TAGTCTCATC AAAATTGAAA GGCTGGGCCG ACATGGCAAA 
GTGCTTTTAA CTGTACCTAG TCCAAGTAGC ACAGCAGAAG AAAAATTCAT TAAGAAGGGT 
GAGGCTACTG GAAGAGATTC TGTTCTAGAT CTAGATCCCG CCAGCATGGT GTTTTATGTT 
GGTGGGGTTC CTTCAGGATT TCAGCTTCCC GCCAGCTTAA ATCTGCCAGG CTTTGTTGGT 
TGTCTAGAGC TTGCTACTGT CAATGATGAT GTTGTTAGCT TGTACAATTT TAAGAACATC 
TACAACATGA ACACAGTCAC AGCACCACCG TGTGCCAGGA ATAAATTGGC CTTCACACAG 
AGCCGTGCAG CAAGCTATTT CTTTGATGGG ACTGGATATG CTGTTATACG CAATATAGAA 
AGGAGGGGTC GCTTTACCCA AGTCACTCGC TTTGATATAG AAGTCCGAAC ACCAGTAGAT 
AATGCCCTTA TTTTACTCAT GGTTAATGGA ACCAAATTCT TTAGTCTGGA GCTCCAAGAT 
GGATATCTGA GACTGCAGTA TGATTTTGGC TTTGCCAAGG GGCCTGTGCT TCTTGAAGAT 
TCAGCGAAGA AGTTTCAGAT AAACGATGCA AGATATCATG AGATCTCAGT CATTTATCAC 
AATTCCAAGA AGATGATATT GGTGGTTGAC AGAAGACATG TGAAGAGTGT TGACAATGAA 
AAGACATCAA TTCCTTTCTC TGACGTTTAT ATTGGAGGAG CGCCTTCTCC AGTTTTGCAG 
AGTGTGAAGA GTCACCTAGC TGCAGACATT TCCTATAAGG GATGCATAAA AGGTTTCCAG 
TTTCAGAAGA AGGATTTCAA TTTGCTGGAA GAACCAGAGA CGCTTGGTAT CAGCTATGGC 
TGTCCTGAAG AATCACTTAT GTCCCGTCGA GCTTATTTTA ATGGGCAAAG TTTTATTGCT 
TCTAGTCAGA AGCTGGCTCC TTTCGAGTCC TTTGAAGGTG GCTTTAGTTT CAAAACTTTA 
CAGCCCAGCG GTCTGTTGCT TTATCACTCT GAAGGGGCTG ATATGTTTTC ATTATCCATG 
GATAAAGGAT CAGTTGTCTT CAGAATAAAA GACATCAAAG TGCAATCTAA AAATAGGAAA 
TACAATGATG GGAAGAACCA CTTTGTGGTT GCTTCAGTCT CCCCAACAAG GTATCAGTTG 
CTGGTAGATG AAACAGATGC AAGTGTAGAA GATAGACAGA AATCTGATGC AAGCTCTCCG 
GTGGCAAGAA AGTTTTATTT TGGAGGCACC CCAACTGGAG TTTCCCAGGC CAATTTCACA 
GGGTGTATCA GCAATGCTTA TTTCACAAGA GTTGACAGAG ATGTTGAAGT TGAGGATTTT 
CAGAAATACA CAGAGAAAGT CCAACCTTCT CTTTATGGGT GTCCAGTTGA AACACCTCCT 
ATTGCTCTGC ATCAAAAGCA TGGTAAAAAT TCCTCTAAAG ACAAAGGAAA GCTAAAGAAG 
AGTGTTGGAA TGGAAAAAAC ATTAGAGTCT CAAGCTGCAA TTGGGTCCAA AGAGACAGAA 
CAAGACTTAG AATTAGTCTC GCAGTGCACC CTCTCTAGCA GACCAAAGGC TGCCAGACAG 
GCACATCTCT ATGGTGGCAT AGCCAACAGC CGACAGGAAT TCAAACAGAT CCCTAATGGC 
TTCAATGAAA AATCTGAACT GTTTGTAAGA CTGAAGACAT ACTCATCCAA TGGAATGATC 
TTCTATGTCT CTGATAAAGA GGAAAACAAT TTTATGACTC TCTTTCTGTC TCATGGCCGA 
CTCACCTTCA TGTTTAACCA TGGGCAGCAA AAGATAAGGA TCAAGTCTCA AGAGAAGTAC 
AACGATGGCT TATGGCACAG TGTGTTATGC ATCCGGGAAA AGAACAAAGG AAGATTAATC 
ATTAATGGGC TCCGTGTATT AGAAGACAGT ACTTCTGTTG CTGCTGTTAT TTGGACTGTG 
GATGCACCAT TTTATCTAGG AGGTGTAGCG CCTGGAATAG CAATCAAAAA TGTTCAGATC 
AACTCAGCGT ACAGTTTCAG TGGCTGCCTT AGCAATTTGC AGATGAATGG ACGCTCTTTG 
ACGTCACCTT CCCAGACATT CAGTGTAACA CCTTGTTTTG AAGGCCCCAT GGAATCTGGA 
ACCTACATTT CATCAGAAGG AGGATATGTG ATTCTTGATG ACTCCTTCAG TACTGGGTTA 
ACATTTGAGT TTGCCCTTGA AGTCCGTCCA CGAAATAACT CTGGAATCTT GATTCACATA 
CAAAATGTGA ATGGAGAGTA TTTGAATTTA CACATGAAGC AGGGACAGGT TGTTGTTAAG 
GTGAAAAATG GCAACAGAGA GTTTTCTACA CATGTCCTAC CAAAACAGAG TCTCTGTGAT 
GGCCAGTGGC ACCGAATTGC AGTGATACGA GATGGAAATG TAGTACAACT GGATGTGGAT 
TCTGAGATAA ACCATGTGGT GGGCCCCATT AACACCAAAG TGCTGGAGCT GAAAGAGCCT 
GTGTTTGTTG GAGGTGTACC AAATTCTTTA CTTGGGCCTA CACTGACTAC ACGCAACTCT 
TTTACTGGCT GTATCCGTAA CTTTGTGATA GATGAACATG CTGTAAGTTT CAATAAAGCA 
TCTCTTGTCA GTGGGGCAGT GAGCATCAAT TCCTGCCCTG CCGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108716825 ( XM_018263293.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018118782.1 Xenopus laevis laminin subunit alpha-4-like (LOC108716825), transcript variant X2, mRNA.

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CloneID	OAf32489
Clone ID Related Accession (Same CDS sequence)	XM_018263293.1
Accession Version	XM_018263293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	laminin subunit alpha-4-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030732.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCCG AGGAGTACCC AAATCTTGTG TGCCATGTCC TTGCCCTCTA CCATTTAGTC 
CTAACAATTT TGCTCTTGCT TGCGGTGTCA AGAGTGGTTC TATGCGCTGT ATCTGCAAGG 
ACAATTATGC TGGGCCAAAC TGTGAAAGAG TGCCGCTGTT CAAAATGTGG TACAGAAACA 
TGCAATGATG TTACCGGGGC ATGTCAATGC AAACCAGGAG TCACTGGTGT TTTCTGTGAT 
CGTTGTGAAA CAGGTCACTA TGGTTATAGC AGTTGCTTGG GCTGTCAGAA ATGTATGTGT 
AGCCTGGCTT CTCTGGATAA CAACTGTGAC CCCGAAACAC GACAGTGTAA TTGTAAGCCT 
GGTGCAGGCG GTCTGAAATG TGAGAGATGC AAGCCAGGAT ACTGGAATTA TGGACCATCT 
GGATGTCAGA AATGTGACTG TGGAGGTGGC CCATGTAACA ATAAAACTGG GGAATGTTTA 
TTAGAGGACC CAGAATCTCC AGCAAATTCC GACTGTTCAT TTGATTGTGA TAAGTGTATA 
TGGGACTTAA TTGATGACCT CAGAATAGCT TCATCACTAG CAGATGAAAC AAAGACTACA 
GCGCTTAGTA TATCAACTGG TGTTGCGGCT CATAAACATC TAAATTACAT CAATTCCACA 
CTGATCCAGC TTACGGAAAT GCTGGAGGAG AAGAACAATC AATCTGTATT TACAGATCTC 
ATGATTGAAA ATGCAGAAAT TAAAGCAGAA GATCTTCAGA CCAAAGTAAA TAAGCTTGCA 
GACAAAGGAA ATCTAGCTGA GATGAAAGGA TCTCGATTTC ACAAAGAAAC CATGGAAACC 
ATGAAACGGG CTAAGTTGAC TGCAGGACAA GTAAATGATA TAGAAGATGG CATCCAAGAA 
CTACTTGGCA AATTAGAATA TTATGAATCA TTGCAAGGAG ACCTGAGCAC CAGTGATCGT 
GTTAAAAGTA AGAAACAAGC AGAGCAAATG CTTGAGGATA TGGAAGAACT GAACTTCAGT 
ACACAAAGGA TTACCCTAGA AGAAGAAAAG GAATATGTTG AAGAGTTGCT GACTGAAATC 
TTAGATGATT GGCAGCAAGT ACAAAACAAT ACCCGATCTT TTCTTCCTAA CGTACTAGAG 
AAAATTGCAG AACACGATGA TGAGCTTGCA GATCTGTATG AAGCAATGGA GGAAGCACGC 
CAACACATCA ACCAAACTAC AGAAAAAAAT AAGGCAAACA CTGGCAAGCT TCAAATGTCC 
AATATTCAAA CAGTAAAGGT GATTAAGGAA GTGGAAAATG TCAGTGGAAC ATTGACACAG 
GCTAACATAA CATTGAATGG CGCAAACATG CTTATTTCTG ACATCAGCGA TATCACCAAG 
AACATGTCTG GACATCATGC TGAAATAGAT GGAGCTTACA TCGGACTAAA AGAAAGACTT 
GCAAATTTGT CTCATAATGT TGAAAATATA GTTGAAGAAG CTGTGACTCA CTCTCTTAAC 
CTTCAAAGAG AAGCAAATGG GCTGACCAGC GACTTAAAGG GAATCGATGC AAATGGATTT 
GTTCAAAAAG CCATTGATGC CTCCAATGTC CATGAGAGCA TTGTTAATAG GATTGAAGCT 
GCTAATGAGA CGTCATTTAT TGCACTAAAT ACAGCAGAAA GAGTTAATGA TGCTTCAGAT 
GGAATGGACA ACCAGATAAA GTATCAGATG ACAGAGAATG AGAAACTGTT GGTTCAAGCT 
AAAGAACTGA AGGAGGCATC TGATTCGAGA AAAGACTTAG GAATTTCAGA AACGAAACAA 
CGTGTAAATG ATGCTGCTCT GAGAAAAGAT GCTTTAAGCG ATAGACTAGA AAATGCCATT 
TCTCAGATTG AAGTACACGA AGATGAGAAC ACAAGAAAGC GACTAGAAAA ATCGAAACTG 
GTTGCTGAGG AAGCCTTAAA CATCACAGCT ATGATATCAA AATTGACCAA TCCAATGTCC 
AAGAATGCCA AGATTTGGAC CAAAGATCTT ATTGATACAG ATTTTGATGC TTCTGCTTAT 
AACAAGGTTG TAAACTCAGC AGGAGAGGCT GTTAGGAACC TCACAGAGGT AGTCCCTTTG 
CTTCTGAATA AGCTGCGAAC AGTAGAATAC AAGTGGCCTA CAAACAATAT ATCATCAAGC 
ATTCTCCGCA TTAGGGAGCT TATAGCACAA ACAAGGAGTG TGGCCAGTAA GGTCCAAGTG 
TCAATGCGGT TTGGAGGTAA ATCAGCTGTT GATGTGAGTC TAAGAACAAA TGTGGCAGAC 
CTGAAAGCAT ATTCTTCTAT CAGTTTGTAC CTAAACCCGA TTGCACACCA GGAAAAACCT 
CATGACAAAC TCATTATGTA TTTCGGAAAT AAGAATTCTA CATCAGACTA CATGGGTCTT 
GCAATTAAGA GCAACAATCT GGTTTATGTG TACAATCTGG GAAGTGGAGA TGTTGAGATT 
CCACTGGATT CCAAGCCTGT CAACTCCTGG CCCGGATATT TTAGTCTCAT CAAAATTGAA 
AGGCTGGGCC GACATGGCAA AGTGCTTTTA ACTGTACCTA GTCCAAGTAG CACAGCAGAA 
GAAAAATTCA TTAAGAAGGG TGAGGCTACT GGAAGAGATT CTGTTCTAGA TCTAGATCCC 
GCCAGCATGG TGTTTTATGT TGGTGGGGTT CCTTCAGGAT TTCAGCTTCC CGCCAGCTTA 
AATCTGCCAG GCTTTGTTGG TTGTCTAGAG CTTGCTACTG TCAATGATGA TGTTGTTAGC 
TTGTACAATT TTAAGAACAT CTACAACATG AACACAGTCA CAGCACCACC GTGTGCCAGG 
AATAAATTGG CCTTCACACA GAGCCGTGCA GCAAGCTATT TCTTTGATGG GACTGGATAT 
GCTGTTATAC GCAATATAGA AAGGAGGGGT CGCTTTACCC AAGTCACTCG CTTTGATATA 
GAAGTCCGAA CACCAGTAGA TAATGCCCTT ATTTTACTCA TGGTTAATGG AACCAAATTC 
TTTAGTCTGG AGCTCCAAGA TGGATATCTG AGACTGCAGT ATGATTTTGG CTTTGCCAAG 
GGGCCTGTGC TTCTTGAAGA TTCAGCGAAG AAGTTTCAGA TAAACGATGC AAGATATCAT 
GAGATCTCAG TCATTTATCA CAATTCCAAG AAGATGATAT TGGTGGTTGA CAGAAGACAT 
GTGAAGAGTG TTGACAATGA AAAGACATCA ATTCCTTTCT CTGACGTTTA TATTGGAGGA 
GCGCCTTCTC CAGTTTTGCA GAGTGTGAAG AGTCACCTAG CTGCAGACAT TTCCTATAAG 
GGATGCATAA AAGGTTTCCA GTTTCAGAAG AAGGATTTCA ATTTGCTGGA AGAACCAGAG 
ACGCTTGGTA TCAGCTATGG CTGTCCTGAA GAATCACTTA TGTCCCGTCG AGCTTATTTT 
AATGGGCAAA GTTTTATTGC TTCTAGTCAG AAGCTGGCTC CTTTCGAGTC CTTTGAAGGT 
GGCTTTAGTT TCAAAACTTT ACAGCCCAGC GGTCTGTTGC TTTATCACTC TGAAGGGGCT 
GATATGTTTT CATTATCCAT GGATAAAGGA TCAGTTGTCT TCAGAATAAA AGACATCAAA 
GTGCAATCTA AAAATAGGAA ATACAATGAT GGGAAGAACC ACTTTGTGGT TGCTTCAGTC 
TCCCCAACAA GGTATCAGTT GCTGGTAGAT GAAACAGATG CAAGTGTAGA AGATAGACAG 
AAATCTGATG CAAGCTCTCC GGTGGCAAGA AAGTTTTATT TTGGAGGCAC CCCAACTGGA 
GTTTCCCAGG CCAATTTCAC AGGGTGTATC AGCAATGCTT ATTTCACAAG AGTTGACAGA 
GATGTTGAAG TTGAGGATTT TCAGAAATAC ACAGAGAAAG TCCAACCTTC TCTTTATGGG 
TGTCCAGTTG AAACACCTCC TATTGCTCTG CATCAAAAGC ATGGTAAAAA TTCCTCTAAA 
GACAAAGGAA AGCTAAAGAA GAGTGTTGGA ATGGAAAAAA CATTAGAGTC TCAAGCTGCA 
ATTGGGTCCA AAGAGACAGA ACAAGACTTA GAATTAGTCT CGCAGTGCAC CCTCTCTAGC 
AGACCAAAGG CTGCCAGACA GGCACATCTC TATGGTGGCA TAGCCAACAG CCGACAGGAA 
TTCAAACAGA TCCCTAATGG CTTCAATGAA AAATCTGAAC TGTTTGTAAG ACTGAAGACA 
TACTCATCCA ATGGAATGAT CTTCTATGTC TCTGATAAAG AGGAAAACAA TTTTATGACT 
CTCTTTCTGT CTCATGGCCG ACTCACCTTC ATGTTTAACC ATGGGCAGCA AAAGATAAGG 
ATCAAGTCTC AAGAGAAGTA CAACGATGGC TTATGGCACA GTGTGTTATG CATCCGGGAA 
AAGAACAAAG GAAGATTAAT CATTAATGGG CTCCGTGTAT TAGAAGACAG TACTTCTGTT 
GCTGCTGTTA TTTGGACTGT GGATGCACCA TTTTATCTAG GAGGTGTAGC GCCTGGAATA 
GCAATCAAAA ATGTTCAGAT CAACTCAGCG TACAGTTTCA GTGGCTGCCT TAGCAATTTG 
CAGATGAATG GACGCTCTTT GACGTCACCT TCCCAGACAT TCAGTGTAAC ACCTTGTTTT 
GAAGGCCCCA TGGAATCTGG AACCTACATT TCATCAGAAG GAGGATATGT GATTCTTGAT 
GACTCCTTCA GTACTGGGTT AACATTTGAG TTTGCCCTTG AAGTCCGTCC ACGAAATAAC 
TCTGGAATCT TGATTCACAT ACAAAATGTG AATGGAGAGT ATTTGAATTT ACACATGAAG 
CAGGGACAGG TTGTTGTTAA GGTGAAAAAT GGCAACAGAG AGTTTTCTAC ACATGTCCTA 
CCAAAACAGA GTCTCTGTGA TGGCCAGTGG CACCGAATTG CAGTGATACG AGATGGAAAT 
GTAGTACAAC TGGATGTGGA TTCTGAGATA AACCATGTGG TGGGCCCCAT TAACACCAAA 
GTGCTGGAGC TGAAAGAGCC TGTGTTTGTT GGAGGTGTAC CAAATTCTTT ACTTGGGCCT 
ACACTGACTA CACGCAACTC TTTTACTGGC TGTATCCGTA ACTTTGTGAT AGATGAACAT 
GCTGTAAGTT TCAATAAAGC ATCTCTTGTC AGTGGGGCAG TGAGCATCAA TTCCTGCCCT 
GCCGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018118782.1
CDS	151..5319
Translation	MWSEEYPNLVCHVLALYHLVLTILLLLAVSRVVLCAVSARTIMLGQTVKECRCSKCGTETCNDVTGACQCKPGVTGVFCDRCETGHYGYSSCLGCQKCMCSLASLDNNCDPETRQCNCKPGAGGLKCERCKPGYWNYGPSGCQKCDCGGGPCNNKTGECLLEDPESPANSDCSFDCDKCIWDLIDDLRIASSLADETKTTALSISTGVAAHKHLNYINSTLIQLTEMLEEKNNQSVFTDLMIENAEIKAEDLQTKVNKLADKGNLAEMKGSRFHKETMETMKRAKLTAGQVNDIEDGIQELLGKLEYYESLQGDLSTSDRVKSKKQAEQMLEDMEELNFSTQRITLEEEKEYVEELLTEILDDWQQVQNNTRSFLPNVLEKIAEHDDELADLYEAMEEARQHINQTTEKNKANTGKLQMSNIQTVKVIKEVENVSGTLTQANITLNGANMLISDISDITKNMSGHHAEIDGAYIGLKERLANLSHNVENIVEEAVTHSLNLQREANGLTSDLKGIDANGFVQKAIDASNVHESIVNRIEAANETSFIALNTAERVNDASDGMDNQIKYQMTENEKLLVQAKELKEASDSRKDLGISETKQRVNDAALRKDALSDRLENAISQIEVHEDENTRKRLEKSKLVAEEALNITAMISKLTNPMSKNAKIWTKDLIDTDFDASAYNKVVNSAGEAVRNLTEVVPLLLNKLRTVEYKWPTNNISSSILRIRELIAQTRSVASKVQVSMRFGGKSAVDVSLRTNVADLKAYSSISLYLNPIAHQEKPHDKLIMYFGNKNSTSDYMGLAIKSNNLVYVYNLGSGDVEIPLDSKPVNSWPGYFSLIKIERLGRHGKVLLTVPSPSSTAEEKFIKKGEATGRDSVLDLDPASMVFYVGGVPSGFQLPASLNLPGFVGCLELATVNDDVVSLYNFKNIYNMNTVTAPPCARNKLAFTQSRAASYFFDGTGYAVIRNIERRGRFTQVTRFDIEVRTPVDNALILLMVNGTKFFSLELQDGYLRLQYDFGFAKGPVLLEDSAKKFQINDARYHEISVIYHNSKKMILVVDRRHVKSVDNEKTSIPFSDVYIGGAPSPVLQSVKSHLAADISYKGCIKGFQFQKKDFNLLEEPETLGISYGCPEESLMSRRAYFNGQSFIASSQKLAPFESFEGGFSFKTLQPSGLLLYHSEGADMFSLSMDKGSVVFRIKDIKVQSKNRKYNDGKNHFVVASVSPTRYQLLVDETDASVEDRQKSDASSPVARKFYFGGTPTGVSQANFTGCISNAYFTRVDRDVEVEDFQKYTEKVQPSLYGCPVETPPIALHQKHGKNSSKDKGKLKKSVGMEKTLESQAAIGSKETEQDLELVSQCTLSSRPKAARQAHLYGGIANSRQEFKQIPNGFNEKSELFVRLKTYSSNGMIFYVSDKEENNFMTLFLSHGRLTFMFNHGQQKIRIKSQEKYNDGLWHSVLCIREKNKGRLIINGLRVLEDSTSVAAVIWTVDAPFYLGGVAPGIAIKNVQINSAYSFSGCLSNLQMNGRSLTSPSQTFSVTPCFEGPMESGTYISSEGGYVILDDSFSTGLTFEFALEVRPRNNSGILIHIQNVNGEYLNLHMKQGQVVVKVKNGNREFSTHVLPKQSLCDGQWHRIAVIRDGNVVQLDVDSEINHVVGPINTKVLELKEPVFVGGVPNSLLGPTLTTRNSFTGCIRNFVIDEHAVSFNKASLVSGAVSINSCPAA

Target ORF information:

RefSeq Version	XM_018263293.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis laminin subunit alpha-4-like (LOC108716825), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018263293.1

ORF Insert Sequence:

ATGTGGTCCG AGGAGTACCC AAATCTTGTG TGCCATGTCC TTGCCCTCTA CCATTTAGTC 
CTAACAATTT TGCTCTTGCT TGCGGTGTCA AGAGTGGTTC TATGCGCTGT ATCTGCAAGG 
ACAATTATGC TGGGCCAAAC TGTGAAAGAG TGCCGCTGTT CAAAATGTGG TACAGAAACA 
TGCAATGATG TTACCGGGGC ATGTCAATGC AAACCAGGAG TCACTGGTGT TTTCTGTGAT 
CGTTGTGAAA CAGGTCACTA TGGTTATAGC AGTTGCTTGG GCTGTCAGAA ATGTATGTGT 
AGCCTGGCTT CTCTGGATAA CAACTGTGAC CCCGAAACAC GACAGTGTAA TTGTAAGCCT 
GGTGCAGGCG GTCTGAAATG TGAGAGATGC AAGCCAGGAT ACTGGAATTA TGGACCATCT 
GGATGTCAGA AATGTGACTG TGGAGGTGGC CCATGTAACA ATAAAACTGG GGAATGTTTA 
TTAGAGGACC CAGAATCTCC AGCAAATTCC GACTGTTCAT TTGATTGTGA TAAGTGTATA 
TGGGACTTAA TTGATGACCT CAGAATAGCT TCATCACTAG CAGATGAAAC AAAGACTACA 
GCGCTTAGTA TATCAACTGG TGTTGCGGCT CATAAACATC TAAATTACAT CAATTCCACA 
CTGATCCAGC TTACGGAAAT GCTGGAGGAG AAGAACAATC AATCTGTATT TACAGATCTC 
ATGATTGAAA ATGCAGAAAT TAAAGCAGAA GATCTTCAGA CCAAAGTAAA TAAGCTTGCA 
GACAAAGGAA ATCTAGCTGA GATGAAAGGA TCTCGATTTC ACAAAGAAAC CATGGAAACC 
ATGAAACGGG CTAAGTTGAC TGCAGGACAA GTAAATGATA TAGAAGATGG CATCCAAGAA 
CTACTTGGCA AATTAGAATA TTATGAATCA TTGCAAGGAG ACCTGAGCAC CAGTGATCGT 
GTTAAAAGTA AGAAACAAGC AGAGCAAATG CTTGAGGATA TGGAAGAACT GAACTTCAGT 
ACACAAAGGA TTACCCTAGA AGAAGAAAAG GAATATGTTG AAGAGTTGCT GACTGAAATC 
TTAGATGATT GGCAGCAAGT ACAAAACAAT ACCCGATCTT TTCTTCCTAA CGTACTAGAG 
AAAATTGCAG AACACGATGA TGAGCTTGCA GATCTGTATG AAGCAATGGA GGAAGCACGC 
CAACACATCA ACCAAACTAC AGAAAAAAAT AAGGCAAACA CTGGCAAGCT TCAAATGTCC 
AATATTCAAA CAGTAAAGGT GATTAAGGAA GTGGAAAATG TCAGTGGAAC ATTGACACAG 
GCTAACATAA CATTGAATGG CGCAAACATG CTTATTTCTG ACATCAGCGA TATCACCAAG 
AACATGTCTG GACATCATGC TGAAATAGAT GGAGCTTACA TCGGACTAAA AGAAAGACTT 
GCAAATTTGT CTCATAATGT TGAAAATATA GTTGAAGAAG CTGTGACTCA CTCTCTTAAC 
CTTCAAAGAG AAGCAAATGG GCTGACCAGC GACTTAAAGG GAATCGATGC AAATGGATTT 
GTTCAAAAAG CCATTGATGC CTCCAATGTC CATGAGAGCA TTGTTAATAG GATTGAAGCT 
GCTAATGAGA CGTCATTTAT TGCACTAAAT ACAGCAGAAA GAGTTAATGA TGCTTCAGAT 
GGAATGGACA ACCAGATAAA GTATCAGATG ACAGAGAATG AGAAACTGTT GGTTCAAGCT 
AAAGAACTGA AGGAGGCATC TGATTCGAGA AAAGACTTAG GAATTTCAGA AACGAAACAA 
CGTGTAAATG ATGCTGCTCT GAGAAAAGAT GCTTTAAGCG ATAGACTAGA AAATGCCATT 
TCTCAGATTG AAGTACACGA AGATGAGAAC ACAAGAAAGC GACTAGAAAA ATCGAAACTG 
GTTGCTGAGG AAGCCTTAAA CATCACAGCT ATGATATCAA AATTGACCAA TCCAATGTCC 
AAGAATGCCA AGATTTGGAC CAAAGATCTT ATTGATACAG ATTTTGATGC TTCTGCTTAT 
AACAAGGTTG TAAACTCAGC AGGAGAGGCT GTTAGGAACC TCACAGAGGT AGTCCCTTTG 
CTTCTGAATA AGCTGCGAAC AGTAGAATAC AAGTGGCCTA CAAACAATAT ATCATCAAGC 
ATTCTCCGCA TTAGGGAGCT TATAGCACAA ACAAGGAGTG TGGCCAGTAA GGTCCAAGTG 
TCAATGCGGT TTGGAGGTAA ATCAGCTGTT GATGTGAGTC TAAGAACAAA TGTGGCAGAC 
CTGAAAGCAT ATTCTTCTAT CAGTTTGTAC CTAAACCCGA TTGCACACCA GGAAAAACCT 
CATGACAAAC TCATTATGTA TTTCGGAAAT AAGAATTCTA CATCAGACTA CATGGGTCTT 
GCAATTAAGA GCAACAATCT GGTTTATGTG TACAATCTGG GAAGTGGAGA TGTTGAGATT 
CCACTGGATT CCAAGCCTGT CAACTCCTGG CCCGGATATT TTAGTCTCAT CAAAATTGAA 
AGGCTGGGCC GACATGGCAA AGTGCTTTTA ACTGTACCTA GTCCAAGTAG CACAGCAGAA 
GAAAAATTCA TTAAGAAGGG TGAGGCTACT GGAAGAGATT CTGTTCTAGA TCTAGATCCC 
GCCAGCATGG TGTTTTATGT TGGTGGGGTT CCTTCAGGAT TTCAGCTTCC CGCCAGCTTA 
AATCTGCCAG GCTTTGTTGG TTGTCTAGAG CTTGCTACTG TCAATGATGA TGTTGTTAGC 
TTGTACAATT TTAAGAACAT CTACAACATG AACACAGTCA CAGCACCACC GTGTGCCAGG 
AATAAATTGG CCTTCACACA GAGCCGTGCA GCAAGCTATT TCTTTGATGG GACTGGATAT 
GCTGTTATAC GCAATATAGA AAGGAGGGGT CGCTTTACCC AAGTCACTCG CTTTGATATA 
GAAGTCCGAA CACCAGTAGA TAATGCCCTT ATTTTACTCA TGGTTAATGG AACCAAATTC 
TTTAGTCTGG AGCTCCAAGA TGGATATCTG AGACTGCAGT ATGATTTTGG CTTTGCCAAG 
GGGCCTGTGC TTCTTGAAGA TTCAGCGAAG AAGTTTCAGA TAAACGATGC AAGATATCAT 
GAGATCTCAG TCATTTATCA CAATTCCAAG AAGATGATAT TGGTGGTTGA CAGAAGACAT 
GTGAAGAGTG TTGACAATGA AAAGACATCA ATTCCTTTCT CTGACGTTTA TATTGGAGGA 
GCGCCTTCTC CAGTTTTGCA GAGTGTGAAG AGTCACCTAG CTGCAGACAT TTCCTATAAG 
GGATGCATAA AAGGTTTCCA GTTTCAGAAG AAGGATTTCA ATTTGCTGGA AGAACCAGAG 
ACGCTTGGTA TCAGCTATGG CTGTCCTGAA GAATCACTTA TGTCCCGTCG AGCTTATTTT 
AATGGGCAAA GTTTTATTGC TTCTAGTCAG AAGCTGGCTC CTTTCGAGTC CTTTGAAGGT 
GGCTTTAGTT TCAAAACTTT ACAGCCCAGC GGTCTGTTGC TTTATCACTC TGAAGGGGCT 
GATATGTTTT CATTATCCAT GGATAAAGGA TCAGTTGTCT TCAGAATAAA AGACATCAAA 
GTGCAATCTA AAAATAGGAA ATACAATGAT GGGAAGAACC ACTTTGTGGT TGCTTCAGTC 
TCCCCAACAA GGTATCAGTT GCTGGTAGAT GAAACAGATG CAAGTGTAGA AGATAGACAG 
AAATCTGATG CAAGCTCTCC GGTGGCAAGA AAGTTTTATT TTGGAGGCAC CCCAACTGGA 
GTTTCCCAGG CCAATTTCAC AGGGTGTATC AGCAATGCTT ATTTCACAAG AGTTGACAGA 
GATGTTGAAG TTGAGGATTT TCAGAAATAC ACAGAGAAAG TCCAACCTTC TCTTTATGGG 
TGTCCAGTTG AAACACCTCC TATTGCTCTG CATCAAAAGC ATGGTAAAAA TTCCTCTAAA 
GACAAAGGAA AGCTAAAGAA GAGTGTTGGA ATGGAAAAAA CATTAGAGTC TCAAGCTGCA 
ATTGGGTCCA AAGAGACAGA ACAAGACTTA GAATTAGTCT CGCAGTGCAC CCTCTCTAGC 
AGACCAAAGG CTGCCAGACA GGCACATCTC TATGGTGGCA TAGCCAACAG CCGACAGGAA 
TTCAAACAGA TCCCTAATGG CTTCAATGAA AAATCTGAAC TGTTTGTAAG ACTGAAGACA 
TACTCATCCA ATGGAATGAT CTTCTATGTC TCTGATAAAG AGGAAAACAA TTTTATGACT 
CTCTTTCTGT CTCATGGCCG ACTCACCTTC ATGTTTAACC ATGGGCAGCA AAAGATAAGG 
ATCAAGTCTC AAGAGAAGTA CAACGATGGC TTATGGCACA GTGTGTTATG CATCCGGGAA 
AAGAACAAAG GAAGATTAAT CATTAATGGG CTCCGTGTAT TAGAAGACAG TACTTCTGTT 
GCTGCTGTTA TTTGGACTGT GGATGCACCA TTTTATCTAG GAGGTGTAGC GCCTGGAATA 
GCAATCAAAA ATGTTCAGAT CAACTCAGCG TACAGTTTCA GTGGCTGCCT TAGCAATTTG 
CAGATGAATG GACGCTCTTT GACGTCACCT TCCCAGACAT TCAGTGTAAC ACCTTGTTTT 
GAAGGCCCCA TGGAATCTGG AACCTACATT TCATCAGAAG GAGGATATGT GATTCTTGAT 
GACTCCTTCA GTACTGGGTT AACATTTGAG TTTGCCCTTG AAGTCCGTCC ACGAAATAAC 
TCTGGAATCT TGATTCACAT ACAAAATGTG AATGGAGAGT ATTTGAATTT ACACATGAAG 
CAGGGACAGG TTGTTGTTAA GGTGAAAAAT GGCAACAGAG AGTTTTCTAC ACATGTCCTA 
CCAAAACAGA GTCTCTGTGA TGGCCAGTGG CACCGAATTG CAGTGATACG AGATGGAAAT 
GTAGTACAAC TGGATGTGGA TTCTGAGATA AACCATGTGG TGGGCCCCAT TAACACCAAA 
GTGCTGGAGC TGAAAGAGCC TGTGTTTGTT GGAGGTGTAC CAAATTCTTT ACTTGGGCCT 
ACACTGACTA CACGCAACTC TTTTACTGGC TGTATCCGTA ACTTTGTGAT AGATGAACAT 
GCTGTAAGTT TCAATAAAGC ATCTCTTGTC AGTGGGGCAG TGAGCATCAA TTCCTGCCCT 
GCCGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC108716825 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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