LOC108712542 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108712542
Entrez Gene ID	108712542
Full Name	DENN/MADD domain containing 2A S homeolog
General protein information	Preferred Names DENN/MADD domain containing 2A S homeolog Names DENN domain-containing protein 2A-like
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018254773.1	XP_018110262.1	DENN domain-containing protein 2A-like isoform X1
XM_018254774.1	XP_018110263.1	DENN domain-containing protein 2A-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC108712542 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108712542 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf26278	XM_018254773.1 Latest version!	Xenopus laevis DENN domain-containing protein 2A-like (LOC108712542), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAf26279	XM_018254774.1 Latest version!	Xenopus laevis DENN domain-containing protein 2A-like (LOC108712542), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108712542 ( XM_018254773.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018110262.1 Xenopus laevis DENN domain-containing protein 2A-like (LOC108712542), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf26278
Clone ID Related Accession (Same CDS sequence)	XM_018254773.1
Accession Version	XM_018254773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	DENN domain-containing protein 2A-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTGA GAAGGGCCAC AGTGGTGACC AGGAATAAAG ACCACTCACC ATACCAGGCC 
GGCTCTAGGG TAAAGCTTGT CAGGAAAGAA AACTCTGAGT CTGGAAGAAA ATCTACAGTT 
CTTCTGGTTG AGAGCCAAAG TGGAATAAAA AGTATTTCTA GGCAAGACTT ATCCCTGCAA 
AAAAGTCCAC TCCAGAGCAT TGATAACCTA TCAGGAGAAG CAGACACAAA CGTTCTAGAC 
TGGAACATGC TCAGCCATAG AATGATCATC AGTGACCAAG TCGTGGACCT CGGTGTCAAG 
GAACAAGGCC ACATCTTAAA GAAACAACTT TCCTCTCCAC CCCAAAAGAT CCCACTAAAG 
AAGACTTCTA GCATCAAGGA CAAGATTTCT GAATGGGAAG AAAAGAAGGA GACACAGTCC 
CCCGTTCTGA GCCGGAAGGA GTCGGTGCAC TTGAAGAGAC AGGATTCCTC AGGCCCAGTT 
TCTGGAGAAA CCAACGGCAA TGGAACAATC CAACCATCTC CTCTTCTCAG CAGGAAGGAG 
CTGGTGCCCA TTAAGAGACA GGATTCTACT GGACTATCTT CTTCAGACAT AGTGGTTCCA 
GAAAGTAAGA TGAGGTCTAA AGGGAGTGTT AAGAGGATGT TTAGTTGGGA GATGGACAAT 
AATCAGAAAG ACTCATCAAA GGTGGAGAAA CCAACTGATA GAGAGACTGA TCAAAATGCT 
GAAAGAGAGA CACCTTCCGG CAACAAGAAG GATCAGACCA CAGTCTTAAA CCAAGTGAAA 
AGGTTTGAGC GCTCCTTGAA AGACAGTCCG GTAGATTTGC AACCACAATT ACCCGGGACC 
TATTATTCTC CACAGTGTCT GCTGGAGGCA GAGAACCAGG AGAACAGAGT CAGGTCAAAA 
AAAACTCATG AAAACTCTGA GACCATTTTT GGGATGGTAG GAGAGGGCAG GCCCTCTAGA 
AGGACTAGCA ATGCTGAGAA TGTATATACA GAACCTGGAG CACAGGAGAA AAGGCTTCCC 
ATAAACCCAC TGCCAAAACC CAGAAGGACT TTCTGTCATG AATGGGAGGG GGAAATTTTT 
CCACCCAGGA CAACTGGGCT CCGTAAAACG AACAGAGACT TGCCTCCTCT TCCAGTTATA 
CTTCCCCCTC CTCTGCCCAC ATCTCCACCT CCATCTGCCA TTAGCAGAAG GCTTTGGAAC 
ACCAAGCACA AGCATAATCG GGACCACAGG AAGTCGTACG AGTTTGAAGA CCTGTTGCAG 
TCGTCCGGGA ACGGCCGAGT GGATTGGTAC GCACAGACCA AGCTCGCTCT CACTCGCACT 
TTATCGGAAG AGAACGTCTA TGAGGATATT CTAGACCCCC CTTCCAAGGA GAACCCCTAT 
GAAGACATTG AGCCGGACAA TCAGTGGCTG GCAAAGAAGA GTGTCCCCGT CTTCCCTAGC 
CCGACCCCCT CTGTCCCAGG GACCCCCACC AAGGTACCTC TGAAGAGCCC TTTCTTCAGG 
CAAAACTCTG AGCGCCGTAG CTTTAAACTC ATGGACTTGA GAAAAATGGG CAGGGACGGA 
GTTTCCTCAC CAAGCAAGCT GAGCCCCCCG TCTTCTCCGA GCAGCCCCGA TGACTCTTTC 
TTCAATATGG GAGACCCCCC AAATGGGCGC AAGAGGAGGA AAATCCCCAG GCTGGTGGTG 
AAAATCAACG ATGTCTTCGA GGCTCGACGG GGTAAAAAAA AAGTGAAGAG GCTTTCACAG 
TCAACAGAGA ACAATTCTGG GAGAGTTACA GATGATAACA GCGAGTCAGA CAGTGACACC 
GAGGAGAAGC TGAAAGCTCA CAGCCAGCGG TTGGTGCATG TCAAGTCCAG AATGAGCCTG 
ACGCCGATGT ATCACACTTT GGAACGGGAC CTGATCGAAT ACCAGGAGCG ACAGTTGTTT 
GAGTACTTTG TGGTGGTCTC GCTGCACAAA AAGCCCACTA GTAGCGTCTA CGTACCTGAG 
GTCACCCAGC AGTTCCCGCT GAAGCTGGAA CGTTCCTTCA AGTTCATGCG AGAGGCTGAG 
GACCAGCTCA AGGCTATTCC ACAGTTCTGC TTTCCCGATG CAAAGGATAG GTCTCCAGTC 
AACCAGTTTA TTAGTGAAAC ATTCTCCTTC GTCCTGACCG GAGAGGACGG CAGTCGGCGG 
TTTGGTTATT GCCGGAGACT CCTGCCCAGT GGGAAAGGGA AGCGCCTGCC GGAAGTTTAT 
TGTATAGTGA GCCGCCTCGG CTGCTTCAAC CTCTTTTCTA AGATCTTGGA TGAAGTTGAG 
AAGAGAAGGG GCCTCTCGCC AGCTCTGGTA CAGCCCTTCA TGAGGAGCAT TATGGAGGCG 
CCATTTCCAG CCCTGGGAAG GAAAATTACT GTGAAGAATT TCCTTCCAGG ATCAGGAACT 
GAGGTGATCG AGCTCTGTCG GCCCCTGGAT TCCCGGCTGG AACATGTTGA CTTTGAAACC 
CTGTTCTCCA CCCTCAGTAT ACGGCACGTC ATACAGATCT TTGCCTCCCT TCTGCTGGAG 
AGACGAGTGA TATTAATGGC AGACACCCTC AGTACCTTGT CCAAGTGCTG CCACGCTATG 
GTTGCCCTTA TCTACCCATT TGCCTGGCAA CACACCTACA TCCCCGTGCT TCCACCTTCC 
ATGATTGACA TTGTGTGCTC CCCCACCCCA TTTCTGATTG GTCTACTCTC CAGTTCTTTG 
AGTCGCTTGA AGGAGTTGCC AGTGGAAGAG GTTTTGCTGG TTGACCTGGG AAATAACCGG 
TTCCTGAGGC AGATGGAGGA TGAAGAGTCT ATCCTACCAA GAAAACTGCA AGCTGCTCTG 
GAGCAAGTTC TGGATCAGAG AAACGAGCTG GCAAGTGACA AGGAGTTGGG TTCAGCCAAT 
GGCCACCAAG ACTCCTGCTC CTTGAACGAC GTTGTGTCGG AAGCCTTTGT CCGATTCTTT 
GTGGAAATTG TGGGCCACTA CTCCCTGTTT CTGGTGCCTG GGGATCAAGA CGAGAAAGCT 
CTGCTCCGCG ATGCCTTCAG AAAGTCTGTC TCATCCAAGA GCCTACGCCG ATTTCTGGAG 
GTCTTCATGG AAACACAGAT GTTTGGGGGT TTCATCCAGG AGAGGGAAAT GAGGAAACAA 
AGCACCAAAG GTCTGTTTGA GGTCCGAGCC CAGGAATACT TGGAGATGCT CCCATGCGGG 
GAGCAGAGCG GGGTCAACAA GTTTCTCAAT AGAATAGGAA ATAAATTCTT TCGGAATAAG 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018110262.1
CDS	429..3674
Translation	MTVRRATVVTRNKDHSPYQAGSRVKLVRKENSESGRKSTVLLVESQSGIKSISRQDLSLQKSPLQSIDNLSGEADTNVLDWNMLSHRMIISDQVVDLGVKEQGHILKKQLSSPPQKIPLKKTSSIKDKISEWEEKKETQSPVLSRKESVHLKRQDSSGPVSGETNGNGTIQPSPLLSRKELVPIKRQDSTGLSSSDIVVPESKMRSKGSVKRMFSWEMDNNQKDSSKVEKPTDRETDQNAERETPSGNKKDQTTVLNQVKRFERSLKDSPVDLQPQLPGTYYSPQCLLEAENQENRVRSKKTHENSETIFGMVGEGRPSRRTSNAENVYTEPGAQEKRLPINPLPKPRRTFCHEWEGEIFPPRTTGLRKTNRDLPPLPVILPPPLPTSPPPSAISRRLWNTKHKHNRDHRKSYEFEDLLQSSGNGRVDWYAQTKLALTRTLSEENVYEDILDPPSKENPYEDIEPDNQWLAKKSVPVFPSPTPSVPGTPTKVPLKSPFFRQNSERRSFKLMDLRKMGRDGVSSPSKLSPPSSPSSPDDSFFNMGDPPNGRKRRKIPRLVVKINDVFEARRGKKKVKRLSQSTENNSGRVTDDNSESDSDTEEKLKAHSQRLVHVKSRMSLTPMYHTLERDLIEYQERQLFEYFVVVSLHKKPTSSVYVPEVTQQFPLKLERSFKFMREAEDQLKAIPQFCFPDAKDRSPVNQFISETFSFVLTGEDGSRRFGYCRRLLPSGKGKRLPEVYCIVSRLGCFNLFSKILDEVEKRRGLSPALVQPFMRSIMEAPFPALGRKITVKNFLPGSGTEVIELCRPLDSRLEHVDFETLFSTLSIRHVIQIFASLLLERRVILMADTLSTLSKCCHAMVALIYPFAWQHTYIPVLPPSMIDIVCSPTPFLIGLLSSSLSRLKELPVEEVLLVDLGNNRFLRQMEDEESILPRKLQAALEQVLDQRNELASDKELGSANGHQDSCSLNDVVSEAFVRFFVEIVGHYSLFLVPGDQDEKALLRDAFRKSVSSKSLRRFLEVFMETQMFGGFIQEREMRKQSTKGLFEVRAQEYLEMLPCGEQSGVNKFLNRIGNKFFRNKK

Target ORF information:

RefSeq Version	XM_018254773.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis DENN domain-containing protein 2A-like (LOC108712542), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018254773.1

ORF Insert Sequence:

ATGACTGTGA GAAGGGCCAC AGTGGTGACC AGGAATAAAG ACCACTCACC ATACCAGGCC 
GGCTCTAGGG TAAAGCTTGT CAGGAAAGAA AACTCTGAGT CTGGAAGAAA ATCTACAGTT 
CTTCTGGTTG AGAGCCAAAG TGGAATAAAA AGTATTTCTA GGCAAGACTT ATCCCTGCAA 
AAAAGTCCAC TCCAGAGCAT TGATAACCTA TCAGGAGAAG CAGACACAAA CGTTCTAGAC 
TGGAACATGC TCAGCCATAG AATGATCATC AGTGACCAAG TCGTGGACCT CGGTGTCAAG 
GAACAAGGCC ACATCTTAAA GAAACAACTT TCCTCTCCAC CCCAAAAGAT CCCACTAAAG 
AAGACTTCTA GCATCAAGGA CAAGATTTCT GAATGGGAAG AAAAGAAGGA GACACAGTCC 
CCCGTTCTGA GCCGGAAGGA GTCGGTGCAC TTGAAGAGAC AGGATTCCTC AGGCCCAGTT 
TCTGGAGAAA CCAACGGCAA TGGAACAATC CAACCATCTC CTCTTCTCAG CAGGAAGGAG 
CTGGTGCCCA TTAAGAGACA GGATTCTACT GGACTATCTT CTTCAGACAT AGTGGTTCCA 
GAAAGTAAGA TGAGGTCTAA AGGGAGTGTT AAGAGGATGT TTAGTTGGGA GATGGACAAT 
AATCAGAAAG ACTCATCAAA GGTGGAGAAA CCAACTGATA GAGAGACTGA TCAAAATGCT 
GAAAGAGAGA CACCTTCCGG CAACAAGAAG GATCAGACCA CAGTCTTAAA CCAAGTGAAA 
AGGTTTGAGC GCTCCTTGAA AGACAGTCCG GTAGATTTGC AACCACAATT ACCCGGGACC 
TATTATTCTC CACAGTGTCT GCTGGAGGCA GAGAACCAGG AGAACAGAGT CAGGTCAAAA 
AAAACTCATG AAAACTCTGA GACCATTTTT GGGATGGTAG GAGAGGGCAG GCCCTCTAGA 
AGGACTAGCA ATGCTGAGAA TGTATATACA GAACCTGGAG CACAGGAGAA AAGGCTTCCC 
ATAAACCCAC TGCCAAAACC CAGAAGGACT TTCTGTCATG AATGGGAGGG GGAAATTTTT 
CCACCCAGGA CAACTGGGCT CCGTAAAACG AACAGAGACT TGCCTCCTCT TCCAGTTATA 
CTTCCCCCTC CTCTGCCCAC ATCTCCACCT CCATCTGCCA TTAGCAGAAG GCTTTGGAAC 
ACCAAGCACA AGCATAATCG GGACCACAGG AAGTCGTACG AGTTTGAAGA CCTGTTGCAG 
TCGTCCGGGA ACGGCCGAGT GGATTGGTAC GCACAGACCA AGCTCGCTCT CACTCGCACT 
TTATCGGAAG AGAACGTCTA TGAGGATATT CTAGACCCCC CTTCCAAGGA GAACCCCTAT 
GAAGACATTG AGCCGGACAA TCAGTGGCTG GCAAAGAAGA GTGTCCCCGT CTTCCCTAGC 
CCGACCCCCT CTGTCCCAGG GACCCCCACC AAGGTACCTC TGAAGAGCCC TTTCTTCAGG 
CAAAACTCTG AGCGCCGTAG CTTTAAACTC ATGGACTTGA GAAAAATGGG CAGGGACGGA 
GTTTCCTCAC CAAGCAAGCT GAGCCCCCCG TCTTCTCCGA GCAGCCCCGA TGACTCTTTC 
TTCAATATGG GAGACCCCCC AAATGGGCGC AAGAGGAGGA AAATCCCCAG GCTGGTGGTG 
AAAATCAACG ATGTCTTCGA GGCTCGACGG GGTAAAAAAA AAGTGAAGAG GCTTTCACAG 
TCAACAGAGA ACAATTCTGG GAGAGTTACA GATGATAACA GCGAGTCAGA CAGTGACACC 
GAGGAGAAGC TGAAAGCTCA CAGCCAGCGG TTGGTGCATG TCAAGTCCAG AATGAGCCTG 
ACGCCGATGT ATCACACTTT GGAACGGGAC CTGATCGAAT ACCAGGAGCG ACAGTTGTTT 
GAGTACTTTG TGGTGGTCTC GCTGCACAAA AAGCCCACTA GTAGCGTCTA CGTACCTGAG 
GTCACCCAGC AGTTCCCGCT GAAGCTGGAA CGTTCCTTCA AGTTCATGCG AGAGGCTGAG 
GACCAGCTCA AGGCTATTCC ACAGTTCTGC TTTCCCGATG CAAAGGATAG GTCTCCAGTC 
AACCAGTTTA TTAGTGAAAC ATTCTCCTTC GTCCTGACCG GAGAGGACGG CAGTCGGCGG 
TTTGGTTATT GCCGGAGACT CCTGCCCAGT GGGAAAGGGA AGCGCCTGCC GGAAGTTTAT 
TGTATAGTGA GCCGCCTCGG CTGCTTCAAC CTCTTTTCTA AGATCTTGGA TGAAGTTGAG 
AAGAGAAGGG GCCTCTCGCC AGCTCTGGTA CAGCCCTTCA TGAGGAGCAT TATGGAGGCG 
CCATTTCCAG CCCTGGGAAG GAAAATTACT GTGAAGAATT TCCTTCCAGG ATCAGGAACT 
GAGGTGATCG AGCTCTGTCG GCCCCTGGAT TCCCGGCTGG AACATGTTGA CTTTGAAACC 
CTGTTCTCCA CCCTCAGTAT ACGGCACGTC ATACAGATCT TTGCCTCCCT TCTGCTGGAG 
AGACGAGTGA TATTAATGGC AGACACCCTC AGTACCTTGT CCAAGTGCTG CCACGCTATG 
GTTGCCCTTA TCTACCCATT TGCCTGGCAA CACACCTACA TCCCCGTGCT TCCACCTTCC 
ATGATTGACA TTGTGTGCTC CCCCACCCCA TTTCTGATTG GTCTACTCTC CAGTTCTTTG 
AGTCGCTTGA AGGAGTTGCC AGTGGAAGAG GTTTTGCTGG TTGACCTGGG AAATAACCGG 
TTCCTGAGGC AGATGGAGGA TGAAGAGTCT ATCCTACCAA GAAAACTGCA AGCTGCTCTG 
GAGCAAGTTC TGGATCAGAG AAACGAGCTG GCAAGTGACA AGGAGTTGGG TTCAGCCAAT 
GGCCACCAAG ACTCCTGCTC CTTGAACGAC GTTGTGTCGG AAGCCTTTGT CCGATTCTTT 
GTGGAAATTG TGGGCCACTA CTCCCTGTTT CTGGTGCCTG GGGATCAAGA CGAGAAAGCT 
CTGCTCCGCG ATGCCTTCAG AAAGTCTGTC TCATCCAAGA GCCTACGCCG ATTTCTGGAG 
GTCTTCATGG AAACACAGAT GTTTGGGGGT TTCATCCAGG AGAGGGAAAT GAGGAAACAA 
AGCACCAAAG GTCTGTTTGA GGTCCGAGCC CAGGAATACT TGGAGATGCT CCCATGCGGG 
GAGCAGAGCG GGGTCAACAA GTTTCTCAAT AGAATAGGAA ATAAATTCTT TCGGAATAAG 
AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108712542 ( XM_018254774.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018110263.1 Xenopus laevis DENN domain-containing protein 2A-like (LOC108712542), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAf26279
Clone ID Related Accession (Same CDS sequence)	XM_018254774.1
Accession Version	XM_018254774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	DENN domain-containing protein 2A-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030729.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTGA GAAGGGCCAC AGTGGTGACC AGGAATAAAG ACCACTCACC ATACCAGGCC 
GGCTCTAGGG TAAAGCTTGT CAGGAAAGAA AACTCTGAGT CTGGAAGAAA ATCTACAGTT 
CTTCTGGTTG AGAGCCAAAG TGGAATAAAA AGTATTTCTA GGCAAGACTT ATCCCTGCAA 
AAAAGTCCAC TCCAGAGCAT TGATAACCTA TCAGGAGAAG CAGACACAAA CGTTCTAGAC 
TGGAACATGC TCAGCCATAG AATGATCATC AGTGACCAAG TCGTGGACCT CGGTGTCAAG 
GAACAAGGCC ACATCTTAAA GAAACAACTT TCCTCTCCAC CCCAAAAGAT CCCACTAAAG 
AAGACTTCTA GCATCAAGGA CAAGATTTCT GAATGGGAAG AAAAGAAGGA GACACAGTCC 
CCCGTTCTGA GCCGGAAGGA GTCGGTGCAC TTGAAGAGAC AGGATTCCTC AGGCCCAGTT 
TCTGGAGAAA CCAACGGCAA TGGAACAATC CAACCATCTC CTCTTCTCAG CAGGAAGGAG 
CTGGTGCCCA TTAAGAGACA GGATTCTACT GGACTATCTT CTTCAGACAT AGTGGTTCCA 
GAAAGTAAGA TGAGGTCTAA AGGGAGTGTT AAGAGGATGT TTAGTTGGGA GATGGACAAT 
AATCAGAAAG ACTCATCAAA GGTGGAGAAA CCAACTGATA GAGAGACTGA TCAAAATGCT 
GAAAGAGAGA CACCTTCCGG CAACAAGAAG GATCAGACCA CAGTCTTAAA CCAAGTGAAA 
AGGTTTGAGC GCTCCTTGAA AGACAGTCCG GTAGATTTGC AACCACAATT ACCCGGGACC 
TATTATTCTC CACAGTGTCT GCTGGAGGCA GAGAACCAGG AGAACAGAGT CAGGTCAAAA 
AAAACTCATG AAAACTCTGA GACCATTTTT GGGATGGTAG GAGAGGGCAG GCCCTCTAGA 
AGGACTAGCA ATGCTGAGAA TGTATATACA GAACCTGGAG CACAGGAGAA AAGGCTTCCC 
ATAAACCCAC TGCCAAAACC CAGAAGGACT TTCTGTCATG AATGGGAGGG GGAAATTTTT 
CCACCCAGGA CAACTGGGCT CCGTAAAACG AACAGAGACT TGCCTCCTCT TCCAGTTATA 
CTTCCCCCTC CTCTGCCCAC ATCTCCACCT CCATCTGCCA TTAGCAGAAG GCTTTGGAAC 
ACCAAGCACA AGCATAATCG GGACCACAGG AAGTCGTACG AGTTTGAAGA CCTGTTGCAG 
TCGTCCGGGA ACGGCCGAGT GGATTGGTAC GCACAGACCA AGCTCGCTCT CACTCGCACT 
TTATCGGAAG AGAACGTCTA TGAGGATATT CTAGACCCCC CTTCCAAGGA GAACCCCTAT 
GAAGACATTG AGCCGGACAA TCAGTGGCTG GCAAAGAAGA GTGTCCCCGT CTTCCCTAGC 
CCGACCCCCT CTGTCCCAGG GACCCCCACC AAGGTACCTC TGAAGAGCCC TTTCTTCAGG 
CAAAACTCTG AGCGCCGTAG CTTTAAACTC ATGGACTTGA GAAAAATGGG CAGGGACGGA 
GTTTCCTCAC CAAGCAAGCT GAGCCCCCCG TCTTCTCCGA GCAGCCCCGA TGACTCTTTC 
TTCAATATGG GAGACCCCCC AAATGGGCGC AAGAGGAGGA AAATCCCCAG GCTGGTGGTG 
AAAATCAACG ATGTCTTCGA GGCTCGACGG GGTAAAAAAA AAGTGAAGAG GCTTTCACAG 
TCAACAGAGA ACAATTCTGG GAGAGATGAT AACAGCGAGT CAGACAGTGA CACCGAGGAG 
AAGCTGAAAG CTCACAGCCA GCGGTTGGTG CATGTCAAGT CCAGAATGAG CCTGACGCCG 
ATGTATCACA CTTTGGAACG GGACCTGATC GAATACCAGG AGCGACAGTT GTTTGAGTAC 
TTTGTGGTGG TCTCGCTGCA CAAAAAGCCC ACTAGTAGCG TCTACGTACC TGAGGTCACC 
CAGCAGTTCC CGCTGAAGCT GGAACGTTCC TTCAAGTTCA TGCGAGAGGC TGAGGACCAG 
CTCAAGGCTA TTCCACAGTT CTGCTTTCCC GATGCAAAGG ATAGGTCTCC AGTCAACCAG 
TTTATTAGTG AAACATTCTC CTTCGTCCTG ACCGGAGAGG ACGGCAGTCG GCGGTTTGGT 
TATTGCCGGA GACTCCTGCC CAGTGGGAAA GGGAAGCGCC TGCCGGAAGT TTATTGTATA 
GTGAGCCGCC TCGGCTGCTT CAACCTCTTT TCTAAGATCT TGGATGAAGT TGAGAAGAGA 
AGGGGCCTCT CGCCAGCTCT GGTACAGCCC TTCATGAGGA GCATTATGGA GGCGCCATTT 
CCAGCCCTGG GAAGGAAAAT TACTGTGAAG AATTTCCTTC CAGGATCAGG AACTGAGGTG 
ATCGAGCTCT GTCGGCCCCT GGATTCCCGG CTGGAACATG TTGACTTTGA AACCCTGTTC 
TCCACCCTCA GTATACGGCA CGTCATACAG ATCTTTGCCT CCCTTCTGCT GGAGAGACGA 
GTGATATTAA TGGCAGACAC CCTCAGTACC TTGTCCAAGT GCTGCCACGC TATGGTTGCC 
CTTATCTACC CATTTGCCTG GCAACACACC TACATCCCCG TGCTTCCACC TTCCATGATT 
GACATTGTGT GCTCCCCCAC CCCATTTCTG ATTGGTCTAC TCTCCAGTTC TTTGAGTCGC 
TTGAAGGAGT TGCCAGTGGA AGAGGTTTTG CTGGTTGACC TGGGAAATAA CCGGTTCCTG 
AGGCAGATGG AGGATGAAGA GTCTATCCTA CCAAGAAAAC TGCAAGCTGC TCTGGAGCAA 
GTTCTGGATC AGAGAAACGA GCTGGCAAGT GACAAGGAGT TGGGTTCAGC CAATGGCCAC 
CAAGACTCCT GCTCCTTGAA CGACGTTGTG TCGGAAGCCT TTGTCCGATT CTTTGTGGAA 
ATTGTGGGCC ACTACTCCCT GTTTCTGGTG CCTGGGGATC AAGACGAGAA AGCTCTGCTC 
CGCGATGCCT TCAGAAAGTC TGTCTCATCC AAGAGCCTAC GCCGATTTCT GGAGGTCTTC 
ATGGAAACAC AGATGTTTGG GGGTTTCATC CAGGAGAGGG AAATGAGGAA ACAAAGCACC 
AAAGGTCTGT TTGAGGTCCG AGCCCAGGAA TACTTGGAGA TGCTCCCATG CGGGGAGCAG 
AGCGGGGTCA ACAAGTTTCT CAATAGAATA GGAAATAAAT TCTTTCGGAA TAAGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018110263.1
CDS	429..3668
Translation	MTVRRATVVTRNKDHSPYQAGSRVKLVRKENSESGRKSTVLLVESQSGIKSISRQDLSLQKSPLQSIDNLSGEADTNVLDWNMLSHRMIISDQVVDLGVKEQGHILKKQLSSPPQKIPLKKTSSIKDKISEWEEKKETQSPVLSRKESVHLKRQDSSGPVSGETNGNGTIQPSPLLSRKELVPIKRQDSTGLSSSDIVVPESKMRSKGSVKRMFSWEMDNNQKDSSKVEKPTDRETDQNAERETPSGNKKDQTTVLNQVKRFERSLKDSPVDLQPQLPGTYYSPQCLLEAENQENRVRSKKTHENSETIFGMVGEGRPSRRTSNAENVYTEPGAQEKRLPINPLPKPRRTFCHEWEGEIFPPRTTGLRKTNRDLPPLPVILPPPLPTSPPPSAISRRLWNTKHKHNRDHRKSYEFEDLLQSSGNGRVDWYAQTKLALTRTLSEENVYEDILDPPSKENPYEDIEPDNQWLAKKSVPVFPSPTPSVPGTPTKVPLKSPFFRQNSERRSFKLMDLRKMGRDGVSSPSKLSPPSSPSSPDDSFFNMGDPPNGRKRRKIPRLVVKINDVFEARRGKKKVKRLSQSTENNSGRDDNSESDSDTEEKLKAHSQRLVHVKSRMSLTPMYHTLERDLIEYQERQLFEYFVVVSLHKKPTSSVYVPEVTQQFPLKLERSFKFMREAEDQLKAIPQFCFPDAKDRSPVNQFISETFSFVLTGEDGSRRFGYCRRLLPSGKGKRLPEVYCIVSRLGCFNLFSKILDEVEKRRGLSPALVQPFMRSIMEAPFPALGRKITVKNFLPGSGTEVIELCRPLDSRLEHVDFETLFSTLSIRHVIQIFASLLLERRVILMADTLSTLSKCCHAMVALIYPFAWQHTYIPVLPPSMIDIVCSPTPFLIGLLSSSLSRLKELPVEEVLLVDLGNNRFLRQMEDEESILPRKLQAALEQVLDQRNELASDKELGSANGHQDSCSLNDVVSEAFVRFFVEIVGHYSLFLVPGDQDEKALLRDAFRKSVSSKSLRRFLEVFMETQMFGGFIQEREMRKQSTKGLFEVRAQEYLEMLPCGEQSGVNKFLNRIGNKFFRNKK

Target ORF information:

RefSeq Version	XM_018254774.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis DENN domain-containing protein 2A-like (LOC108712542), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018254774.1

ORF Insert Sequence:

ATGACTGTGA GAAGGGCCAC AGTGGTGACC AGGAATAAAG ACCACTCACC ATACCAGGCC 
GGCTCTAGGG TAAAGCTTGT CAGGAAAGAA AACTCTGAGT CTGGAAGAAA ATCTACAGTT 
CTTCTGGTTG AGAGCCAAAG TGGAATAAAA AGTATTTCTA GGCAAGACTT ATCCCTGCAA 
AAAAGTCCAC TCCAGAGCAT TGATAACCTA TCAGGAGAAG CAGACACAAA CGTTCTAGAC 
TGGAACATGC TCAGCCATAG AATGATCATC AGTGACCAAG TCGTGGACCT CGGTGTCAAG 
GAACAAGGCC ACATCTTAAA GAAACAACTT TCCTCTCCAC CCCAAAAGAT CCCACTAAAG 
AAGACTTCTA GCATCAAGGA CAAGATTTCT GAATGGGAAG AAAAGAAGGA GACACAGTCC 
CCCGTTCTGA GCCGGAAGGA GTCGGTGCAC TTGAAGAGAC AGGATTCCTC AGGCCCAGTT 
TCTGGAGAAA CCAACGGCAA TGGAACAATC CAACCATCTC CTCTTCTCAG CAGGAAGGAG 
CTGGTGCCCA TTAAGAGACA GGATTCTACT GGACTATCTT CTTCAGACAT AGTGGTTCCA 
GAAAGTAAGA TGAGGTCTAA AGGGAGTGTT AAGAGGATGT TTAGTTGGGA GATGGACAAT 
AATCAGAAAG ACTCATCAAA GGTGGAGAAA CCAACTGATA GAGAGACTGA TCAAAATGCT 
GAAAGAGAGA CACCTTCCGG CAACAAGAAG GATCAGACCA CAGTCTTAAA CCAAGTGAAA 
AGGTTTGAGC GCTCCTTGAA AGACAGTCCG GTAGATTTGC AACCACAATT ACCCGGGACC 
TATTATTCTC CACAGTGTCT GCTGGAGGCA GAGAACCAGG AGAACAGAGT CAGGTCAAAA 
AAAACTCATG AAAACTCTGA GACCATTTTT GGGATGGTAG GAGAGGGCAG GCCCTCTAGA 
AGGACTAGCA ATGCTGAGAA TGTATATACA GAACCTGGAG CACAGGAGAA AAGGCTTCCC 
ATAAACCCAC TGCCAAAACC CAGAAGGACT TTCTGTCATG AATGGGAGGG GGAAATTTTT 
CCACCCAGGA CAACTGGGCT CCGTAAAACG AACAGAGACT TGCCTCCTCT TCCAGTTATA 
CTTCCCCCTC CTCTGCCCAC ATCTCCACCT CCATCTGCCA TTAGCAGAAG GCTTTGGAAC 
ACCAAGCACA AGCATAATCG GGACCACAGG AAGTCGTACG AGTTTGAAGA CCTGTTGCAG 
TCGTCCGGGA ACGGCCGAGT GGATTGGTAC GCACAGACCA AGCTCGCTCT CACTCGCACT 
TTATCGGAAG AGAACGTCTA TGAGGATATT CTAGACCCCC CTTCCAAGGA GAACCCCTAT 
GAAGACATTG AGCCGGACAA TCAGTGGCTG GCAAAGAAGA GTGTCCCCGT CTTCCCTAGC 
CCGACCCCCT CTGTCCCAGG GACCCCCACC AAGGTACCTC TGAAGAGCCC TTTCTTCAGG 
CAAAACTCTG AGCGCCGTAG CTTTAAACTC ATGGACTTGA GAAAAATGGG CAGGGACGGA 
GTTTCCTCAC CAAGCAAGCT GAGCCCCCCG TCTTCTCCGA GCAGCCCCGA TGACTCTTTC 
TTCAATATGG GAGACCCCCC AAATGGGCGC AAGAGGAGGA AAATCCCCAG GCTGGTGGTG 
AAAATCAACG ATGTCTTCGA GGCTCGACGG GGTAAAAAAA AAGTGAAGAG GCTTTCACAG 
TCAACAGAGA ACAATTCTGG GAGAGATGAT AACAGCGAGT CAGACAGTGA CACCGAGGAG 
AAGCTGAAAG CTCACAGCCA GCGGTTGGTG CATGTCAAGT CCAGAATGAG CCTGACGCCG 
ATGTATCACA CTTTGGAACG GGACCTGATC GAATACCAGG AGCGACAGTT GTTTGAGTAC 
TTTGTGGTGG TCTCGCTGCA CAAAAAGCCC ACTAGTAGCG TCTACGTACC TGAGGTCACC 
CAGCAGTTCC CGCTGAAGCT GGAACGTTCC TTCAAGTTCA TGCGAGAGGC TGAGGACCAG 
CTCAAGGCTA TTCCACAGTT CTGCTTTCCC GATGCAAAGG ATAGGTCTCC AGTCAACCAG 
TTTATTAGTG AAACATTCTC CTTCGTCCTG ACCGGAGAGG ACGGCAGTCG GCGGTTTGGT 
TATTGCCGGA GACTCCTGCC CAGTGGGAAA GGGAAGCGCC TGCCGGAAGT TTATTGTATA 
GTGAGCCGCC TCGGCTGCTT CAACCTCTTT TCTAAGATCT TGGATGAAGT TGAGAAGAGA 
AGGGGCCTCT CGCCAGCTCT GGTACAGCCC TTCATGAGGA GCATTATGGA GGCGCCATTT 
CCAGCCCTGG GAAGGAAAAT TACTGTGAAG AATTTCCTTC CAGGATCAGG AACTGAGGTG 
ATCGAGCTCT GTCGGCCCCT GGATTCCCGG CTGGAACATG TTGACTTTGA AACCCTGTTC 
TCCACCCTCA GTATACGGCA CGTCATACAG ATCTTTGCCT CCCTTCTGCT GGAGAGACGA 
GTGATATTAA TGGCAGACAC CCTCAGTACC TTGTCCAAGT GCTGCCACGC TATGGTTGCC 
CTTATCTACC CATTTGCCTG GCAACACACC TACATCCCCG TGCTTCCACC TTCCATGATT 
GACATTGTGT GCTCCCCCAC CCCATTTCTG ATTGGTCTAC TCTCCAGTTC TTTGAGTCGC 
TTGAAGGAGT TGCCAGTGGA AGAGGTTTTG CTGGTTGACC TGGGAAATAA CCGGTTCCTG 
AGGCAGATGG AGGATGAAGA GTCTATCCTA CCAAGAAAAC TGCAAGCTGC TCTGGAGCAA 
GTTCTGGATC AGAGAAACGA GCTGGCAAGT GACAAGGAGT TGGGTTCAGC CAATGGCCAC 
CAAGACTCCT GCTCCTTGAA CGACGTTGTG TCGGAAGCCT TTGTCCGATT CTTTGTGGAA 
ATTGTGGGCC ACTACTCCCT GTTTCTGGTG CCTGGGGATC AAGACGAGAA AGCTCTGCTC 
CGCGATGCCT TCAGAAAGTC TGTCTCATCC AAGAGCCTAC GCCGATTTCT GGAGGTCTTC 
ATGGAAACAC AGATGTTTGG GGGTTTCATC CAGGAGAGGG AAATGAGGAA ACAAAGCACC 
AAAGGTCTGT TTGAGGTCCG AGCCCAGGAA TACTTGGAGA TGCTCCCATG CGGGGAGCAG 
AGCGGGGTCA ACAAGTTTCT CAATAGAATA GGAAATAAAT TCTTTCGGAA TAAGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC108712542 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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