LOC108699932 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108699932
Entrez Gene ID	108699932
Full Name	TSC complex subunit 1 S homeolog
Synonyms	hamartin,lam,tsc
General protein information	Preferred Names TSC complex subunit 1 S homeolog Names hamartin-like tuberous sclerosis 1 S homeolog
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018232630.1	XP_018088119.1	hamartin-like isoform X1
XM_018232636.1	XP_018088125.1	hamartin-like isoform X6
XM_018232633.1	XP_018088122.1	hamartin-like isoform X3
XM_018232634.1	XP_018088123.1	hamartin-like isoform X4
XM_018232632.1	XP_018088121.1	hamartin-like isoform X2
XM_018232635.1	XP_018088124.1	hamartin-like isoform X5
XM_018232631.1	XP_018088120.1	hamartin-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC108699932 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108699932 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAf43884	XM_018232631.1 Latest version!	Xenopus laevis hamartin-like (LOC108699932), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAf43884	XM_018232630.1 Latest version!	Xenopus laevis hamartin-like (LOC108699932), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAf43885	XM_018232632.1 Latest version!	Xenopus laevis hamartin-like (LOC108699932), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAf43886	XM_018232633.1 Latest version!	Xenopus laevis hamartin-like (LOC108699932), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAf43887	XM_018232634.1 Latest version!	Xenopus laevis hamartin-like (LOC108699932), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAf43888	XM_018232635.1 Latest version!	Xenopus laevis hamartin-like (LOC108699932), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAf43889	XM_018232636.1 Latest version!	Xenopus laevis hamartin-like (LOC108699932), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108699932 ( XM_018232630.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088119.1 Xenopus laevis hamartin-like (LOC108699932), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf43884
Clone ID Related Accession (Same CDS sequence)	XM_018232630.1 , XM_018232631.1
Accession Version	XM_018232630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	hamartin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GGTTCAAAGA AACGTATCCT GTTTCAGCTT GGCTCCATAC 
GAGCATCTCT TTGAACTGGC GTTGCCCAAA ACCGCTAACC TTTTTCTCTG CAATAAGACA 
GAGGAGCTGT GCAGAAGAGG CAAGGGAAGC CTTGAGGAGG GTGAAGAGGG TGCCTTCTCC 
ATTTCCCCAG CGGAAGTGCT TGACCGACTT ATCCAGTTGG GAGCAGATGC CCACACCAAA 
GAGCTGAGCA ACAGGTTGTC TTTACCCAGC AAAGCCGCTG ACTGGACCCA CTTTGGAGGT 
TCCCCACCAA CAGATGAAAT CCATGTACTT CGCAATCAGT TGGTTCTGCT TCACAACCAG 
CTTTTGTATG AAAGGTTCAA AAGGCAGCAG CATGCCCAAC GCAACCGCCG CCTCCTGCGC 
AAAGTTATCA AGGTGACTTC ACTGGAGGAA CAGAATGCTG CTATGAAAAA ACAGTTAAAG 
CTTCAGGAGG TGGACATGCA TTCTATCAAA CAGAGCCTCT TTCAGGAACA GACGCGGTAC 
CGGCGGCTTC AGGAAGAGCG AGACACTGTG GTCACTCAAC TGCGCAGCCA GATCCGCCAG 
CTACAGCATG ACAGAGATCT GTTCTATAAC CAAAGCCAGG AGCTGCAGAC CAAGCTAGAG 
GATTGCCGCA CCACCATTGC AGATTTACGT CTGGAGGTGA AACAAGCCAA TAATAAAGTG 
TGCCATACGG AGCTCCGTCT AAGCCAGGTT TCTCAAAAGC TCTCCAACAG TGAGTCTGTA 
CAGCATCAGA TGGAGTTTCT GAACCGTCAG CTCCTGGTCC TGGGGGAGGT AAACGAGTTG 
TACGTGGAGC AGTTGCAGCA AAAGCAGACA AACAGCACAA AGGAAGTGGA GATGTTGCAG 
ACTTCCTTTA AAAAGGACCT GGAGAAGGCT CGATTGAATG TTGTGCAACT GAGTCAGAGG 
CTAGAGTGCA CCCAGAAACG AATAGTGGAG CTGGAAGCTC AGCTGGCCAA GAAAGAATCC 
TTGTTTCAAG AACAGAAAAA ATTCTTAGAG CATGTTAAGG GGAAGAGCAG AGGCCAGCTG 
CAAGCAGCCG AGAGCAGATA CCTCGCTCAG AAGAGGATAA GCCAGGTCTT CGAAGTCAAA 
ATCCTCAGTC TGTACAACAG GTTGGAGAAG GAAGGGCCTC TGAAAAGTCA AAGTGAAGAG 
AAAGCGGAGA CCGCAGAAGC CGCAGAAGAA AGGTGTGACA TTAACATTGC AAGTGTTTAC 
AGTCCAGATG TTGGCATTCT AGAAGAAGTC AATGGTAGCG AATATAACTT GCCTGCCAAC 
CGCACAAGCT CCTCCAGTAG CCAGAGCAGC AGCACCCTCT CCACCCCAGA GAAAGGCACA 
AACTTACAGC TCAGCCGTCG GCAGTTACTA GAGATCCCTT CCAGCGGCCA CTTGACTATC 
GGCTCCTACC CCAACGCCAA AAGTTTCCTA GGCATCCGGG CCCGCGAGCT GTTCCGCAAC 
AAGAGCGAGA GCCACTGCGA CGAGGAAAGC ATCAGCAGCC TCTCGGAGAT TCTGAAAACT 
GAACTCTGCC AGGAGCTTAT CCCAGAGACG AATGCGGAGG GCTCCCCCCA AAATCAAGAT 
TGTGTGAGGA ATCTTCAGAT TATGGACTAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088119.1
CDS	443..3778
Translation	MAQQTTVWEHLYLLESSDLQQLDQIHTLIRDHLIADRSPILVNNLVDHYVESGSEQAMLILSMVQEPHDKHLLDKINEYLVKAASRLPTLTLLGHVIRRQPSWKHKLSQAPVLQSLLKCLKVDNDVVVLTTGVLVLITVLPMIPQSGKQHLYEFFDIFGRLATWCLKNPGRVAEMCLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEIVKPMMEHVRIHPELVTGSKDHELDPSRWKRLETHDIVVECAKISLDPSEASYEEGYSPLSNRLRQRQHDTTSSPYTEIRGSNASTPRTCTQSAPPHTASIELLQDSIWSPSTICGMTTPPNSPANVLVDASHASSRPPSKVLYTPGGKATPLGTPATSPPPTSNSEECTPASGASLTPHKKNESSDPGRPATNRQLLSKGEKSTDESPKTQKTVYLSELPDIISDFAADHNSTEEELKKNGHAAICQELSRITMPDPEPTVLRGGFDSPFSLRCDSQPGSQKKLASYSFGLIPEPSATDKLHYSVSHDNSKQAFTPIEPPNGNENATDSGKPPSNNSLPSTADIFSPQVQRNVSCFSLAPYEHLFELALPKTANLFLCNKTEELCRRGKGSLEEGEEGAFSISPAEVLDRLIQLGADAHTKELSNRLSLPSKAADWTHFGGSPPTDEIHVLRNQLVLLHNQLLYERFKRQQHAQRNRRLLRKVIKVTSLEEQNAAMKKQLKLQEVDMHSIKQSLFQEQTRYRRLQEERDTVVTQLRSQIRQLQHDRDLFYNQSQELQTKLEDCRTTIADLRLEVKQANNKVCHTELRLSQVSQKLSNSESVQHQMEFLNRQLLVLGEVNELYVEQLQQKQTNSTKEVEMLQTSFKKDLEKARLNVVQLSQRLECTQKRIVELEAQLAKKESLFQEQKKFLEHVKGKSRGQLQAAESRYLAQKRISQVFEVKILSLYNRLEKEGPLKSQSEEKAETAEAAEERCDINIASVYSPDVGILEEVNGSEYNLPANRTSSSSSQSSSTLSTPEKGTNLQLSRRQLLEIPSSGHLTIGSYPNAKSFLGIRARELFRNKSESHCDEESISSLSEILKTELCQELIPETNAEGSPQNQDCVRNLQIMDYD

Target ORF information:

RefSeq Version	XM_018232630.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis hamartin-like (LOC108699932), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232630.1

ORF Insert Sequence:

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GGTTCAAAGA AACGTATCCT GTTTCAGCTT GGCTCCATAC 
GAGCATCTCT TTGAACTGGC GTTGCCCAAA ACCGCTAACC TTTTTCTCTG CAATAAGACA 
GAGGAGCTGT GCAGAAGAGG CAAGGGAAGC CTTGAGGAGG GTGAAGAGGG TGCCTTCTCC 
ATTTCCCCAG CGGAAGTGCT TGACCGACTT ATCCAGTTGG GAGCAGATGC CCACACCAAA 
GAGCTGAGCA ACAGGTTGTC TTTACCCAGC AAAGCCGCTG ACTGGACCCA CTTTGGAGGT 
TCCCCACCAA CAGATGAAAT CCATGTACTT CGCAATCAGT TGGTTCTGCT TCACAACCAG 
CTTTTGTATG AAAGGTTCAA AAGGCAGCAG CATGCCCAAC GCAACCGCCG CCTCCTGCGC 
AAAGTTATCA AGGTGACTTC ACTGGAGGAA CAGAATGCTG CTATGAAAAA ACAGTTAAAG 
CTTCAGGAGG TGGACATGCA TTCTATCAAA CAGAGCCTCT TTCAGGAACA GACGCGGTAC 
CGGCGGCTTC AGGAAGAGCG AGACACTGTG GTCACTCAAC TGCGCAGCCA GATCCGCCAG 
CTACAGCATG ACAGAGATCT GTTCTATAAC CAAAGCCAGG AGCTGCAGAC CAAGCTAGAG 
GATTGCCGCA CCACCATTGC AGATTTACGT CTGGAGGTGA AACAAGCCAA TAATAAAGTG 
TGCCATACGG AGCTCCGTCT AAGCCAGGTT TCTCAAAAGC TCTCCAACAG TGAGTCTGTA 
CAGCATCAGA TGGAGTTTCT GAACCGTCAG CTCCTGGTCC TGGGGGAGGT AAACGAGTTG 
TACGTGGAGC AGTTGCAGCA AAAGCAGACA AACAGCACAA AGGAAGTGGA GATGTTGCAG 
ACTTCCTTTA AAAAGGACCT GGAGAAGGCT CGATTGAATG TTGTGCAACT GAGTCAGAGG 
CTAGAGTGCA CCCAGAAACG AATAGTGGAG CTGGAAGCTC AGCTGGCCAA GAAAGAATCC 
TTGTTTCAAG AACAGAAAAA ATTCTTAGAG CATGTTAAGG GGAAGAGCAG AGGCCAGCTG 
CAAGCAGCCG AGAGCAGATA CCTCGCTCAG AAGAGGATAA GCCAGGTCTT CGAAGTCAAA 
ATCCTCAGTC TGTACAACAG GTTGGAGAAG GAAGGGCCTC TGAAAAGTCA AAGTGAAGAG 
AAAGCGGAGA CCGCAGAAGC CGCAGAAGAA AGGTGTGACA TTAACATTGC AAGTGTTTAC 
AGTCCAGATG TTGGCATTCT AGAAGAAGTC AATGGTAGCG AATATAACTT GCCTGCCAAC 
CGCACAAGCT CCTCCAGTAG CCAGAGCAGC AGCACCCTCT CCACCCCAGA GAAAGGCACA 
AACTTACAGC TCAGCCGTCG GCAGTTACTA GAGATCCCTT CCAGCGGCCA CTTGACTATC 
GGCTCCTACC CCAACGCCAA AAGTTTCCTA GGCATCCGGG CCCGCGAGCT GTTCCGCAAC 
AAGAGCGAGA GCCACTGCGA CGAGGAAAGC ATCAGCAGCC TCTCGGAGAT TCTGAAAACT 
GAACTCTGCC AGGAGCTTAT CCCAGAGACG AATGCGGAGG GCTCCCCCCA AAATCAAGAT 
TGTGTGAGGA ATCTTCAGAT TATGGACTAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108699932 ( XM_018232631.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088120.1 Xenopus laevis hamartin-like (LOC108699932), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAf43884
Clone ID Related Accession (Same CDS sequence)	XM_018232630.1 , XM_018232631.1
Accession Version	XM_018232631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	hamartin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GGTTCAAAGA AACGTATCCT GTTTCAGCTT GGCTCCATAC 
GAGCATCTCT TTGAACTGGC GTTGCCCAAA ACCGCTAACC TTTTTCTCTG CAATAAGACA 
GAGGAGCTGT GCAGAAGAGG CAAGGGAAGC CTTGAGGAGG GTGAAGAGGG TGCCTTCTCC 
ATTTCCCCAG CGGAAGTGCT TGACCGACTT ATCCAGTTGG GAGCAGATGC CCACACCAAA 
GAGCTGAGCA ACAGGTTGTC TTTACCCAGC AAAGCCGCTG ACTGGACCCA CTTTGGAGGT 
TCCCCACCAA CAGATGAAAT CCATGTACTT CGCAATCAGT TGGTTCTGCT TCACAACCAG 
CTTTTGTATG AAAGGTTCAA AAGGCAGCAG CATGCCCAAC GCAACCGCCG CCTCCTGCGC 
AAAGTTATCA AGGTGACTTC ACTGGAGGAA CAGAATGCTG CTATGAAAAA ACAGTTAAAG 
CTTCAGGAGG TGGACATGCA TTCTATCAAA CAGAGCCTCT TTCAGGAACA GACGCGGTAC 
CGGCGGCTTC AGGAAGAGCG AGACACTGTG GTCACTCAAC TGCGCAGCCA GATCCGCCAG 
CTACAGCATG ACAGAGATCT GTTCTATAAC CAAAGCCAGG AGCTGCAGAC CAAGCTAGAG 
GATTGCCGCA CCACCATTGC AGATTTACGT CTGGAGGTGA AACAAGCCAA TAATAAAGTG 
TGCCATACGG AGCTCCGTCT AAGCCAGGTT TCTCAAAAGC TCTCCAACAG TGAGTCTGTA 
CAGCATCAGA TGGAGTTTCT GAACCGTCAG CTCCTGGTCC TGGGGGAGGT AAACGAGTTG 
TACGTGGAGC AGTTGCAGCA AAAGCAGACA AACAGCACAA AGGAAGTGGA GATGTTGCAG 
ACTTCCTTTA AAAAGGACCT GGAGAAGGCT CGATTGAATG TTGTGCAACT GAGTCAGAGG 
CTAGAGTGCA CCCAGAAACG AATAGTGGAG CTGGAAGCTC AGCTGGCCAA GAAAGAATCC 
TTGTTTCAAG AACAGAAAAA ATTCTTAGAG CATGTTAAGG GGAAGAGCAG AGGCCAGCTG 
CAAGCAGCCG AGAGCAGATA CCTCGCTCAG AAGAGGATAA GCCAGGTCTT CGAAGTCAAA 
ATCCTCAGTC TGTACAACAG GTTGGAGAAG GAAGGGCCTC TGAAAAGTCA AAGTGAAGAG 
AAAGCGGAGA CCGCAGAAGC CGCAGAAGAA AGGTGTGACA TTAACATTGC AAGTGTTTAC 
AGTCCAGATG TTGGCATTCT AGAAGAAGTC AATGGTAGCG AATATAACTT GCCTGCCAAC 
CGCACAAGCT CCTCCAGTAG CCAGAGCAGC AGCACCCTCT CCACCCCAGA GAAAGGCACA 
AACTTACAGC TCAGCCGTCG GCAGTTACTA GAGATCCCTT CCAGCGGCCA CTTGACTATC 
GGCTCCTACC CCAACGCCAA AAGTTTCCTA GGCATCCGGG CCCGCGAGCT GTTCCGCAAC 
AAGAGCGAGA GCCACTGCGA CGAGGAAAGC ATCAGCAGCC TCTCGGAGAT TCTGAAAACT 
GAACTCTGCC AGGAGCTTAT CCCAGAGACG AATGCGGAGG GCTCCCCCCA AAATCAAGAT 
TGTGTGAGGA ATCTTCAGAT TATGGACTAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088120.1
CDS	485..3820
Translation	MAQQTTVWEHLYLLESSDLQQLDQIHTLIRDHLIADRSPILVNNLVDHYVESGSEQAMLILSMVQEPHDKHLLDKINEYLVKAASRLPTLTLLGHVIRRQPSWKHKLSQAPVLQSLLKCLKVDNDVVVLTTGVLVLITVLPMIPQSGKQHLYEFFDIFGRLATWCLKNPGRVAEMCLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEIVKPMMEHVRIHPELVTGSKDHELDPSRWKRLETHDIVVECAKISLDPSEASYEEGYSPLSNRLRQRQHDTTSSPYTEIRGSNASTPRTCTQSAPPHTASIELLQDSIWSPSTICGMTTPPNSPANVLVDASHASSRPPSKVLYTPGGKATPLGTPATSPPPTSNSEECTPASGASLTPHKKNESSDPGRPATNRQLLSKGEKSTDESPKTQKTVYLSELPDIISDFAADHNSTEEELKKNGHAAICQELSRITMPDPEPTVLRGGFDSPFSLRCDSQPGSQKKLASYSFGLIPEPSATDKLHYSVSHDNSKQAFTPIEPPNGNENATDSGKPPSNNSLPSTADIFSPQVQRNVSCFSLAPYEHLFELALPKTANLFLCNKTEELCRRGKGSLEEGEEGAFSISPAEVLDRLIQLGADAHTKELSNRLSLPSKAADWTHFGGSPPTDEIHVLRNQLVLLHNQLLYERFKRQQHAQRNRRLLRKVIKVTSLEEQNAAMKKQLKLQEVDMHSIKQSLFQEQTRYRRLQEERDTVVTQLRSQIRQLQHDRDLFYNQSQELQTKLEDCRTTIADLRLEVKQANNKVCHTELRLSQVSQKLSNSESVQHQMEFLNRQLLVLGEVNELYVEQLQQKQTNSTKEVEMLQTSFKKDLEKARLNVVQLSQRLECTQKRIVELEAQLAKKESLFQEQKKFLEHVKGKSRGQLQAAESRYLAQKRISQVFEVKILSLYNRLEKEGPLKSQSEEKAETAEAAEERCDINIASVYSPDVGILEEVNGSEYNLPANRTSSSSSQSSSTLSTPEKGTNLQLSRRQLLEIPSSGHLTIGSYPNAKSFLGIRARELFRNKSESHCDEESISSLSEILKTELCQELIPETNAEGSPQNQDCVRNLQIMDYD

Target ORF information:

RefSeq Version	XM_018232631.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis hamartin-like (LOC108699932), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232631.1

ORF Insert Sequence:

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GGTTCAAAGA AACGTATCCT GTTTCAGCTT GGCTCCATAC 
GAGCATCTCT TTGAACTGGC GTTGCCCAAA ACCGCTAACC TTTTTCTCTG CAATAAGACA 
GAGGAGCTGT GCAGAAGAGG CAAGGGAAGC CTTGAGGAGG GTGAAGAGGG TGCCTTCTCC 
ATTTCCCCAG CGGAAGTGCT TGACCGACTT ATCCAGTTGG GAGCAGATGC CCACACCAAA 
GAGCTGAGCA ACAGGTTGTC TTTACCCAGC AAAGCCGCTG ACTGGACCCA CTTTGGAGGT 
TCCCCACCAA CAGATGAAAT CCATGTACTT CGCAATCAGT TGGTTCTGCT TCACAACCAG 
CTTTTGTATG AAAGGTTCAA AAGGCAGCAG CATGCCCAAC GCAACCGCCG CCTCCTGCGC 
AAAGTTATCA AGGTGACTTC ACTGGAGGAA CAGAATGCTG CTATGAAAAA ACAGTTAAAG 
CTTCAGGAGG TGGACATGCA TTCTATCAAA CAGAGCCTCT TTCAGGAACA GACGCGGTAC 
CGGCGGCTTC AGGAAGAGCG AGACACTGTG GTCACTCAAC TGCGCAGCCA GATCCGCCAG 
CTACAGCATG ACAGAGATCT GTTCTATAAC CAAAGCCAGG AGCTGCAGAC CAAGCTAGAG 
GATTGCCGCA CCACCATTGC AGATTTACGT CTGGAGGTGA AACAAGCCAA TAATAAAGTG 
TGCCATACGG AGCTCCGTCT AAGCCAGGTT TCTCAAAAGC TCTCCAACAG TGAGTCTGTA 
CAGCATCAGA TGGAGTTTCT GAACCGTCAG CTCCTGGTCC TGGGGGAGGT AAACGAGTTG 
TACGTGGAGC AGTTGCAGCA AAAGCAGACA AACAGCACAA AGGAAGTGGA GATGTTGCAG 
ACTTCCTTTA AAAAGGACCT GGAGAAGGCT CGATTGAATG TTGTGCAACT GAGTCAGAGG 
CTAGAGTGCA CCCAGAAACG AATAGTGGAG CTGGAAGCTC AGCTGGCCAA GAAAGAATCC 
TTGTTTCAAG AACAGAAAAA ATTCTTAGAG CATGTTAAGG GGAAGAGCAG AGGCCAGCTG 
CAAGCAGCCG AGAGCAGATA CCTCGCTCAG AAGAGGATAA GCCAGGTCTT CGAAGTCAAA 
ATCCTCAGTC TGTACAACAG GTTGGAGAAG GAAGGGCCTC TGAAAAGTCA AAGTGAAGAG 
AAAGCGGAGA CCGCAGAAGC CGCAGAAGAA AGGTGTGACA TTAACATTGC AAGTGTTTAC 
AGTCCAGATG TTGGCATTCT AGAAGAAGTC AATGGTAGCG AATATAACTT GCCTGCCAAC 
CGCACAAGCT CCTCCAGTAG CCAGAGCAGC AGCACCCTCT CCACCCCAGA GAAAGGCACA 
AACTTACAGC TCAGCCGTCG GCAGTTACTA GAGATCCCTT CCAGCGGCCA CTTGACTATC 
GGCTCCTACC CCAACGCCAA AAGTTTCCTA GGCATCCGGG CCCGCGAGCT GTTCCGCAAC 
AAGAGCGAGA GCCACTGCGA CGAGGAAAGC ATCAGCAGCC TCTCGGAGAT TCTGAAAACT 
GAACTCTGCC AGGAGCTTAT CCCAGAGACG AATGCGGAGG GCTCCCCCCA AAATCAAGAT 
TGTGTGAGGA ATCTTCAGAT TATGGACTAT GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108699932 ( XM_018232632.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088121.1 Xenopus laevis hamartin-like (LOC108699932), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAf43885
Clone ID Related Accession (Same CDS sequence)	XM_018232632.1
Accession Version	XM_018232632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	hamartin-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GGTTCAAAGA AACGTATCCT GTTTCAGCTT GGCTCCATAC 
GAGCATCTCT TTGAACTGGC GTTGCCCAAA ACCGCTAACC TTTTTCTCTG CAATAAGACA 
GAGGAGCTGT GCAGAAGAGG CAAGGGAAGC CTTGAGGAGG GTGAAGAGGG TGCCTTCTCC 
ATTTCCCCAG CGGAAGTGCT TGACCGACTT ATCCAGTTGG GAGCAGATGC CCACACCAAA 
GAGCTGAGCA AGTTGTCTTT ACCCAGCAAA GCCGCTGACT GGACCCACTT TGGAGGTTCC 
CCACCAACAG ATGAAATCCA TGTACTTCGC AATCAGTTGG TTCTGCTTCA CAACCAGCTT 
TTGTATGAAA GGTTCAAAAG GCAGCAGCAT GCCCAACGCA ACCGCCGCCT CCTGCGCAAA 
GTTATCAAGG TGACTTCACT GGAGGAACAG AATGCTGCTA TGAAAAAACA GTTAAAGCTT 
CAGGAGGTGG ACATGCATTC TATCAAACAG AGCCTCTTTC AGGAACAGAC GCGGTACCGG 
CGGCTTCAGG AAGAGCGAGA CACTGTGGTC ACTCAACTGC GCAGCCAGAT CCGCCAGCTA 
CAGCATGACA GAGATCTGTT CTATAACCAA AGCCAGGAGC TGCAGACCAA GCTAGAGGAT 
TGCCGCACCA CCATTGCAGA TTTACGTCTG GAGGTGAAAC AAGCCAATAA TAAAGTGTGC 
CATACGGAGC TCCGTCTAAG CCAGGTTTCT CAAAAGCTCT CCAACAGTGA GTCTGTACAG 
CATCAGATGG AGTTTCTGAA CCGTCAGCTC CTGGTCCTGG GGGAGGTAAA CGAGTTGTAC 
GTGGAGCAGT TGCAGCAAAA GCAGACAAAC AGCACAAAGG AAGTGGAGAT GTTGCAGACT 
TCCTTTAAAA AGGACCTGGA GAAGGCTCGA TTGAATGTTG TGCAACTGAG TCAGAGGCTA 
GAGTGCACCC AGAAACGAAT AGTGGAGCTG GAAGCTCAGC TGGCCAAGAA AGAATCCTTG 
TTTCAAGAAC AGAAAAAATT CTTAGAGCAT GTTAAGGGGA AGAGCAGAGG CCAGCTGCAA 
GCAGCCGAGA GCAGATACCT CGCTCAGAAG AGGATAAGCC AGGTCTTCGA AGTCAAAATC 
CTCAGTCTGT ACAACAGGTT GGAGAAGGAA GGGCCTCTGA AAAGTCAAAG TGAAGAGAAA 
GCGGAGACCG CAGAAGCCGC AGAAGAAAGG TGTGACATTA ACATTGCAAG TGTTTACAGT 
CCAGATGTTG GCATTCTAGA AGAAGTCAAT GGTAGCGAAT ATAACTTGCC TGCCAACCGC 
ACAAGCTCCT CCAGTAGCCA GAGCAGCAGC ACCCTCTCCA CCCCAGAGAA AGGCACAAAC 
TTACAGCTCA GCCGTCGGCA GTTACTAGAG ATCCCTTCCA GCGGCCACTT GACTATCGGC 
TCCTACCCCA ACGCCAAAAG TTTCCTAGGC ATCCGGGCCC GCGAGCTGTT CCGCAACAAG 
AGCGAGAGCC ACTGCGACGA GGAAAGCATC AGCAGCCTCT CGGAGATTCT GAAAACTGAA 
CTCTGCCAGG AGCTTATCCC AGAGACGAAT GCGGAGGGCT CCCCCCAAAA TCAAGATTGT 
GTGAGGAATC TTCAGATTAT GGACTATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088121.1
CDS	444..3776
Translation	MAQQTTVWEHLYLLESSDLQQLDQIHTLIRDHLIADRSPILVNNLVDHYVESGSEQAMLILSMVQEPHDKHLLDKINEYLVKAASRLPTLTLLGHVIRRQPSWKHKLSQAPVLQSLLKCLKVDNDVVVLTTGVLVLITVLPMIPQSGKQHLYEFFDIFGRLATWCLKNPGRVAEMCLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEIVKPMMEHVRIHPELVTGSKDHELDPSRWKRLETHDIVVECAKISLDPSEASYEEGYSPLSNRLRQRQHDTTSSPYTEIRGSNASTPRTCTQSAPPHTASIELLQDSIWSPSTICGMTTPPNSPANVLVDASHASSRPPSKVLYTPGGKATPLGTPATSPPPTSNSEECTPASGASLTPHKKNESSDPGRPATNRQLLSKGEKSTDESPKTQKTVYLSELPDIISDFAADHNSTEEELKKNGHAAICQELSRITMPDPEPTVLRGGFDSPFSLRCDSQPGSQKKLASYSFGLIPEPSATDKLHYSVSHDNSKQAFTPIEPPNGNENATDSGKPPSNNSLPSTADIFSPQVQRNVSCFSLAPYEHLFELALPKTANLFLCNKTEELCRRGKGSLEEGEEGAFSISPAEVLDRLIQLGADAHTKELSKLSLPSKAADWTHFGGSPPTDEIHVLRNQLVLLHNQLLYERFKRQQHAQRNRRLLRKVIKVTSLEEQNAAMKKQLKLQEVDMHSIKQSLFQEQTRYRRLQEERDTVVTQLRSQIRQLQHDRDLFYNQSQELQTKLEDCRTTIADLRLEVKQANNKVCHTELRLSQVSQKLSNSESVQHQMEFLNRQLLVLGEVNELYVEQLQQKQTNSTKEVEMLQTSFKKDLEKARLNVVQLSQRLECTQKRIVELEAQLAKKESLFQEQKKFLEHVKGKSRGQLQAAESRYLAQKRISQVFEVKILSLYNRLEKEGPLKSQSEEKAETAEAAEERCDINIASVYSPDVGILEEVNGSEYNLPANRTSSSSSQSSSTLSTPEKGTNLQLSRRQLLEIPSSGHLTIGSYPNAKSFLGIRARELFRNKSESHCDEESISSLSEILKTELCQELIPETNAEGSPQNQDCVRNLQIMDYD

Target ORF information:

RefSeq Version	XM_018232632.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis hamartin-like (LOC108699932), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232632.1

ORF Insert Sequence:

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GGTTCAAAGA AACGTATCCT GTTTCAGCTT GGCTCCATAC 
GAGCATCTCT TTGAACTGGC GTTGCCCAAA ACCGCTAACC TTTTTCTCTG CAATAAGACA 
GAGGAGCTGT GCAGAAGAGG CAAGGGAAGC CTTGAGGAGG GTGAAGAGGG TGCCTTCTCC 
ATTTCCCCAG CGGAAGTGCT TGACCGACTT ATCCAGTTGG GAGCAGATGC CCACACCAAA 
GAGCTGAGCA AGTTGTCTTT ACCCAGCAAA GCCGCTGACT GGACCCACTT TGGAGGTTCC 
CCACCAACAG ATGAAATCCA TGTACTTCGC AATCAGTTGG TTCTGCTTCA CAACCAGCTT 
TTGTATGAAA GGTTCAAAAG GCAGCAGCAT GCCCAACGCA ACCGCCGCCT CCTGCGCAAA 
GTTATCAAGG TGACTTCACT GGAGGAACAG AATGCTGCTA TGAAAAAACA GTTAAAGCTT 
CAGGAGGTGG ACATGCATTC TATCAAACAG AGCCTCTTTC AGGAACAGAC GCGGTACCGG 
CGGCTTCAGG AAGAGCGAGA CACTGTGGTC ACTCAACTGC GCAGCCAGAT CCGCCAGCTA 
CAGCATGACA GAGATCTGTT CTATAACCAA AGCCAGGAGC TGCAGACCAA GCTAGAGGAT 
TGCCGCACCA CCATTGCAGA TTTACGTCTG GAGGTGAAAC AAGCCAATAA TAAAGTGTGC 
CATACGGAGC TCCGTCTAAG CCAGGTTTCT CAAAAGCTCT CCAACAGTGA GTCTGTACAG 
CATCAGATGG AGTTTCTGAA CCGTCAGCTC CTGGTCCTGG GGGAGGTAAA CGAGTTGTAC 
GTGGAGCAGT TGCAGCAAAA GCAGACAAAC AGCACAAAGG AAGTGGAGAT GTTGCAGACT 
TCCTTTAAAA AGGACCTGGA GAAGGCTCGA TTGAATGTTG TGCAACTGAG TCAGAGGCTA 
GAGTGCACCC AGAAACGAAT AGTGGAGCTG GAAGCTCAGC TGGCCAAGAA AGAATCCTTG 
TTTCAAGAAC AGAAAAAATT CTTAGAGCAT GTTAAGGGGA AGAGCAGAGG CCAGCTGCAA 
GCAGCCGAGA GCAGATACCT CGCTCAGAAG AGGATAAGCC AGGTCTTCGA AGTCAAAATC 
CTCAGTCTGT ACAACAGGTT GGAGAAGGAA GGGCCTCTGA AAAGTCAAAG TGAAGAGAAA 
GCGGAGACCG CAGAAGCCGC AGAAGAAAGG TGTGACATTA ACATTGCAAG TGTTTACAGT 
CCAGATGTTG GCATTCTAGA AGAAGTCAAT GGTAGCGAAT ATAACTTGCC TGCCAACCGC 
ACAAGCTCCT CCAGTAGCCA GAGCAGCAGC ACCCTCTCCA CCCCAGAGAA AGGCACAAAC 
TTACAGCTCA GCCGTCGGCA GTTACTAGAG ATCCCTTCCA GCGGCCACTT GACTATCGGC 
TCCTACCCCA ACGCCAAAAG TTTCCTAGGC ATCCGGGCCC GCGAGCTGTT CCGCAACAAG 
AGCGAGAGCC ACTGCGACGA GGAAAGCATC AGCAGCCTCT CGGAGATTCT GAAAACTGAA 
CTCTGCCAGG AGCTTATCCC AGAGACGAAT GCGGAGGGCT CCCCCCAAAA TCAAGATTGT 
GTGAGGAATC TTCAGATTAT GGACTATGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108699932 ( XM_018232633.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088122.1 Xenopus laevis hamartin-like (LOC108699932), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAf43886
Clone ID Related Accession (Same CDS sequence)	XM_018232633.1
Accession Version	XM_018232633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	hamartin-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GGTTCAAAGA AACGTATCCT GTTTCAGCTT GGCTCCATAC 
GAGCATCTCT TTGAACTGGC GTTGCCCAAA ACCGCTAACC TTTTTCTCTG CAATAAGACA 
GAGGAGCTGT GCAGAAGAGG CAAGGGAAGC CTTGAGGAGG GTGAAGAGGG TGCCTTCTCC 
ATTTCCCCAG CGGAAGTGCT TGACCGACTT ATCCAGTTGG GAGCAGATGC CCACACCAAA 
GAGCTGAGCA ACAGGTTGTC TTTACCCAGC AAAGCCGCTG ACTGGACCCA CTTTGGAGAT 
GAAATCCATG TACTTCGCAA TCAGTTGGTT CTGCTTCACA ACCAGCTTTT GTATGAAAGG 
TTCAAAAGGC AGCAGCATGC CCAACGCAAC CGCCGCCTCC TGCGCAAAGT TATCAAGGTG 
ACTTCACTGG AGGAACAGAA TGCTGCTATG AAAAAACAGT TAAAGCTTCA GGAGGTGGAC 
ATGCATTCTA TCAAACAGAG CCTCTTTCAG GAACAGACGC GGTACCGGCG GCTTCAGGAA 
GAGCGAGACA CTGTGGTCAC TCAACTGCGC AGCCAGATCC GCCAGCTACA GCATGACAGA 
GATCTGTTCT ATAACCAAAG CCAGGAGCTG CAGACCAAGC TAGAGGATTG CCGCACCACC 
ATTGCAGATT TACGTCTGGA GGTGAAACAA GCCAATAATA AAGTGTGCCA TACGGAGCTC 
CGTCTAAGCC AGGTTTCTCA AAAGCTCTCC AACAGTGAGT CTGTACAGCA TCAGATGGAG 
TTTCTGAACC GTCAGCTCCT GGTCCTGGGG GAGGTAAACG AGTTGTACGT GGAGCAGTTG 
CAGCAAAAGC AGACAAACAG CACAAAGGAA GTGGAGATGT TGCAGACTTC CTTTAAAAAG 
GACCTGGAGA AGGCTCGATT GAATGTTGTG CAACTGAGTC AGAGGCTAGA GTGCACCCAG 
AAACGAATAG TGGAGCTGGA AGCTCAGCTG GCCAAGAAAG AATCCTTGTT TCAAGAACAG 
AAAAAATTCT TAGAGCATGT TAAGGGGAAG AGCAGAGGCC AGCTGCAAGC AGCCGAGAGC 
AGATACCTCG CTCAGAAGAG GATAAGCCAG GTCTTCGAAG TCAAAATCCT CAGTCTGTAC 
AACAGGTTGG AGAAGGAAGG GCCTCTGAAA AGTCAAAGTG AAGAGAAAGC GGAGACCGCA 
GAAGCCGCAG AAGAAAGGTG TGACATTAAC ATTGCAAGTG TTTACAGTCC AGATGTTGGC 
ATTCTAGAAG AAGTCAATGG TAGCGAATAT AACTTGCCTG CCAACCGCAC AAGCTCCTCC 
AGTAGCCAGA GCAGCAGCAC CCTCTCCACC CCAGAGAAAG GCACAAACTT ACAGCTCAGC 
CGTCGGCAGT TACTAGAGAT CCCTTCCAGC GGCCACTTGA CTATCGGCTC CTACCCCAAC 
GCCAAAAGTT TCCTAGGCAT CCGGGCCCGC GAGCTGTTCC GCAACAAGAG CGAGAGCCAC 
TGCGACGAGG AAAGCATCAG CAGCCTCTCG GAGATTCTGA AAACTGAACT CTGCCAGGAG 
CTTATCCCAG AGACGAATGC GGAGGGCTCC CCCCAAAATC AAGATTGTGT GAGGAATCTT 
CAGATTATGG ACTATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088122.1
CDS	444..3764
Translation	MAQQTTVWEHLYLLESSDLQQLDQIHTLIRDHLIADRSPILVNNLVDHYVESGSEQAMLILSMVQEPHDKHLLDKINEYLVKAASRLPTLTLLGHVIRRQPSWKHKLSQAPVLQSLLKCLKVDNDVVVLTTGVLVLITVLPMIPQSGKQHLYEFFDIFGRLATWCLKNPGRVAEMCLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEIVKPMMEHVRIHPELVTGSKDHELDPSRWKRLETHDIVVECAKISLDPSEASYEEGYSPLSNRLRQRQHDTTSSPYTEIRGSNASTPRTCTQSAPPHTASIELLQDSIWSPSTICGMTTPPNSPANVLVDASHASSRPPSKVLYTPGGKATPLGTPATSPPPTSNSEECTPASGASLTPHKKNESSDPGRPATNRQLLSKGEKSTDESPKTQKTVYLSELPDIISDFAADHNSTEEELKKNGHAAICQELSRITMPDPEPTVLRGGFDSPFSLRCDSQPGSQKKLASYSFGLIPEPSATDKLHYSVSHDNSKQAFTPIEPPNGNENATDSGKPPSNNSLPSTADIFSPQVQRNVSCFSLAPYEHLFELALPKTANLFLCNKTEELCRRGKGSLEEGEEGAFSISPAEVLDRLIQLGADAHTKELSNRLSLPSKAADWTHFGDEIHVLRNQLVLLHNQLLYERFKRQQHAQRNRRLLRKVIKVTSLEEQNAAMKKQLKLQEVDMHSIKQSLFQEQTRYRRLQEERDTVVTQLRSQIRQLQHDRDLFYNQSQELQTKLEDCRTTIADLRLEVKQANNKVCHTELRLSQVSQKLSNSESVQHQMEFLNRQLLVLGEVNELYVEQLQQKQTNSTKEVEMLQTSFKKDLEKARLNVVQLSQRLECTQKRIVELEAQLAKKESLFQEQKKFLEHVKGKSRGQLQAAESRYLAQKRISQVFEVKILSLYNRLEKEGPLKSQSEEKAETAEAAEERCDINIASVYSPDVGILEEVNGSEYNLPANRTSSSSSQSSSTLSTPEKGTNLQLSRRQLLEIPSSGHLTIGSYPNAKSFLGIRARELFRNKSESHCDEESISSLSEILKTELCQELIPETNAEGSPQNQDCVRNLQIMDYD

Target ORF information:

RefSeq Version	XM_018232633.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis hamartin-like (LOC108699932), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232633.1

ORF Insert Sequence:

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GGTTCAAAGA AACGTATCCT GTTTCAGCTT GGCTCCATAC 
GAGCATCTCT TTGAACTGGC GTTGCCCAAA ACCGCTAACC TTTTTCTCTG CAATAAGACA 
GAGGAGCTGT GCAGAAGAGG CAAGGGAAGC CTTGAGGAGG GTGAAGAGGG TGCCTTCTCC 
ATTTCCCCAG CGGAAGTGCT TGACCGACTT ATCCAGTTGG GAGCAGATGC CCACACCAAA 
GAGCTGAGCA ACAGGTTGTC TTTACCCAGC AAAGCCGCTG ACTGGACCCA CTTTGGAGAT 
GAAATCCATG TACTTCGCAA TCAGTTGGTT CTGCTTCACA ACCAGCTTTT GTATGAAAGG 
TTCAAAAGGC AGCAGCATGC CCAACGCAAC CGCCGCCTCC TGCGCAAAGT TATCAAGGTG 
ACTTCACTGG AGGAACAGAA TGCTGCTATG AAAAAACAGT TAAAGCTTCA GGAGGTGGAC 
ATGCATTCTA TCAAACAGAG CCTCTTTCAG GAACAGACGC GGTACCGGCG GCTTCAGGAA 
GAGCGAGACA CTGTGGTCAC TCAACTGCGC AGCCAGATCC GCCAGCTACA GCATGACAGA 
GATCTGTTCT ATAACCAAAG CCAGGAGCTG CAGACCAAGC TAGAGGATTG CCGCACCACC 
ATTGCAGATT TACGTCTGGA GGTGAAACAA GCCAATAATA AAGTGTGCCA TACGGAGCTC 
CGTCTAAGCC AGGTTTCTCA AAAGCTCTCC AACAGTGAGT CTGTACAGCA TCAGATGGAG 
TTTCTGAACC GTCAGCTCCT GGTCCTGGGG GAGGTAAACG AGTTGTACGT GGAGCAGTTG 
CAGCAAAAGC AGACAAACAG CACAAAGGAA GTGGAGATGT TGCAGACTTC CTTTAAAAAG 
GACCTGGAGA AGGCTCGATT GAATGTTGTG CAACTGAGTC AGAGGCTAGA GTGCACCCAG 
AAACGAATAG TGGAGCTGGA AGCTCAGCTG GCCAAGAAAG AATCCTTGTT TCAAGAACAG 
AAAAAATTCT TAGAGCATGT TAAGGGGAAG AGCAGAGGCC AGCTGCAAGC AGCCGAGAGC 
AGATACCTCG CTCAGAAGAG GATAAGCCAG GTCTTCGAAG TCAAAATCCT CAGTCTGTAC 
AACAGGTTGG AGAAGGAAGG GCCTCTGAAA AGTCAAAGTG AAGAGAAAGC GGAGACCGCA 
GAAGCCGCAG AAGAAAGGTG TGACATTAAC ATTGCAAGTG TTTACAGTCC AGATGTTGGC 
ATTCTAGAAG AAGTCAATGG TAGCGAATAT AACTTGCCTG CCAACCGCAC AAGCTCCTCC 
AGTAGCCAGA GCAGCAGCAC CCTCTCCACC CCAGAGAAAG GCACAAACTT ACAGCTCAGC 
CGTCGGCAGT TACTAGAGAT CCCTTCCAGC GGCCACTTGA CTATCGGCTC CTACCCCAAC 
GCCAAAAGTT TCCTAGGCAT CCGGGCCCGC GAGCTGTTCC GCAACAAGAG CGAGAGCCAC 
TGCGACGAGG AAAGCATCAG CAGCCTCTCG GAGATTCTGA AAACTGAACT CTGCCAGGAG 
CTTATCCCAG AGACGAATGC GGAGGGCTCC CCCCAAAATC AAGATTGTGT GAGGAATCTT 
CAGATTATGG ACTATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108699932 ( XM_018232634.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088123.1 Xenopus laevis hamartin-like (LOC108699932), transcript variant X5, mRNA.

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CloneID	OAf43887
Clone ID Related Accession (Same CDS sequence)	XM_018232634.1
Accession Version	XM_018232634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	hamartin-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GGTTCAAAGA AACGTATCCT GTTTCAGCTT GGCTCCATAC 
GAGCATCTCT TTGAACTGGC GTTGCCCAAA ACCGCTAACC TTTTTCTCTG CAATAAGACA 
GAGGAGCTGT GCAGAAGAGG CAAGGGAAGC CTTGAGGAGG GTGAAGAGGG TGCCTTCTCC 
ATTTCCCCAG CGGAAGTGCT TGACCGACTT ATCCAGTTGG GAGCAGATGC CCACACCAAA 
GAGCTGAGCA AGTTGTCTTT ACCCAGCAAA GCCGCTGACT GGACCCACTT TGGAGATGAA 
ATCCATGTAC TTCGCAATCA GTTGGTTCTG CTTCACAACC AGCTTTTGTA TGAAAGGTTC 
AAAAGGCAGC AGCATGCCCA ACGCAACCGC CGCCTCCTGC GCAAAGTTAT CAAGGTGACT 
TCACTGGAGG AACAGAATGC TGCTATGAAA AAACAGTTAA AGCTTCAGGA GGTGGACATG 
CATTCTATCA AACAGAGCCT CTTTCAGGAA CAGACGCGGT ACCGGCGGCT TCAGGAAGAG 
CGAGACACTG TGGTCACTCA ACTGCGCAGC CAGATCCGCC AGCTACAGCA TGACAGAGAT 
CTGTTCTATA ACCAAAGCCA GGAGCTGCAG ACCAAGCTAG AGGATTGCCG CACCACCATT 
GCAGATTTAC GTCTGGAGGT GAAACAAGCC AATAATAAAG TGTGCCATAC GGAGCTCCGT 
CTAAGCCAGG TTTCTCAAAA GCTCTCCAAC AGTGAGTCTG TACAGCATCA GATGGAGTTT 
CTGAACCGTC AGCTCCTGGT CCTGGGGGAG GTAAACGAGT TGTACGTGGA GCAGTTGCAG 
CAAAAGCAGA CAAACAGCAC AAAGGAAGTG GAGATGTTGC AGACTTCCTT TAAAAAGGAC 
CTGGAGAAGG CTCGATTGAA TGTTGTGCAA CTGAGTCAGA GGCTAGAGTG CACCCAGAAA 
CGAATAGTGG AGCTGGAAGC TCAGCTGGCC AAGAAAGAAT CCTTGTTTCA AGAACAGAAA 
AAATTCTTAG AGCATGTTAA GGGGAAGAGC AGAGGCCAGC TGCAAGCAGC CGAGAGCAGA 
TACCTCGCTC AGAAGAGGAT AAGCCAGGTC TTCGAAGTCA AAATCCTCAG TCTGTACAAC 
AGGTTGGAGA AGGAAGGGCC TCTGAAAAGT CAAAGTGAAG AGAAAGCGGA GACCGCAGAA 
GCCGCAGAAG AAAGGTGTGA CATTAACATT GCAAGTGTTT ACAGTCCAGA TGTTGGCATT 
CTAGAAGAAG TCAATGGTAG CGAATATAAC TTGCCTGCCA ACCGCACAAG CTCCTCCAGT 
AGCCAGAGCA GCAGCACCCT CTCCACCCCA GAGAAAGGCA CAAACTTACA GCTCAGCCGT 
CGGCAGTTAC TAGAGATCCC TTCCAGCGGC CACTTGACTA TCGGCTCCTA CCCCAACGCC 
AAAAGTTTCC TAGGCATCCG GGCCCGCGAG CTGTTCCGCA ACAAGAGCGA GAGCCACTGC 
GACGAGGAAA GCATCAGCAG CCTCTCGGAG ATTCTGAAAA CTGAACTCTG CCAGGAGCTT 
ATCCCAGAGA CGAATGCGGA GGGCTCCCCC CAAAATCAAG ATTGTGTGAG GAATCTTCAG 
ATTATGGACT ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088123.1
CDS	444..3761
Translation	MAQQTTVWEHLYLLESSDLQQLDQIHTLIRDHLIADRSPILVNNLVDHYVESGSEQAMLILSMVQEPHDKHLLDKINEYLVKAASRLPTLTLLGHVIRRQPSWKHKLSQAPVLQSLLKCLKVDNDVVVLTTGVLVLITVLPMIPQSGKQHLYEFFDIFGRLATWCLKNPGRVAEMCLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEIVKPMMEHVRIHPELVTGSKDHELDPSRWKRLETHDIVVECAKISLDPSEASYEEGYSPLSNRLRQRQHDTTSSPYTEIRGSNASTPRTCTQSAPPHTASIELLQDSIWSPSTICGMTTPPNSPANVLVDASHASSRPPSKVLYTPGGKATPLGTPATSPPPTSNSEECTPASGASLTPHKKNESSDPGRPATNRQLLSKGEKSTDESPKTQKTVYLSELPDIISDFAADHNSTEEELKKNGHAAICQELSRITMPDPEPTVLRGGFDSPFSLRCDSQPGSQKKLASYSFGLIPEPSATDKLHYSVSHDNSKQAFTPIEPPNGNENATDSGKPPSNNSLPSTADIFSPQVQRNVSCFSLAPYEHLFELALPKTANLFLCNKTEELCRRGKGSLEEGEEGAFSISPAEVLDRLIQLGADAHTKELSKLSLPSKAADWTHFGDEIHVLRNQLVLLHNQLLYERFKRQQHAQRNRRLLRKVIKVTSLEEQNAAMKKQLKLQEVDMHSIKQSLFQEQTRYRRLQEERDTVVTQLRSQIRQLQHDRDLFYNQSQELQTKLEDCRTTIADLRLEVKQANNKVCHTELRLSQVSQKLSNSESVQHQMEFLNRQLLVLGEVNELYVEQLQQKQTNSTKEVEMLQTSFKKDLEKARLNVVQLSQRLECTQKRIVELEAQLAKKESLFQEQKKFLEHVKGKSRGQLQAAESRYLAQKRISQVFEVKILSLYNRLEKEGPLKSQSEEKAETAEAAEERCDINIASVYSPDVGILEEVNGSEYNLPANRTSSSSSQSSSTLSTPEKGTNLQLSRRQLLEIPSSGHLTIGSYPNAKSFLGIRARELFRNKSESHCDEESISSLSEILKTELCQELIPETNAEGSPQNQDCVRNLQIMDYD

Target ORF information:

RefSeq Version	XM_018232634.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis hamartin-like (LOC108699932), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232634.1

ORF Insert Sequence:

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GGTTCAAAGA AACGTATCCT GTTTCAGCTT GGCTCCATAC 
GAGCATCTCT TTGAACTGGC GTTGCCCAAA ACCGCTAACC TTTTTCTCTG CAATAAGACA 
GAGGAGCTGT GCAGAAGAGG CAAGGGAAGC CTTGAGGAGG GTGAAGAGGG TGCCTTCTCC 
ATTTCCCCAG CGGAAGTGCT TGACCGACTT ATCCAGTTGG GAGCAGATGC CCACACCAAA 
GAGCTGAGCA AGTTGTCTTT ACCCAGCAAA GCCGCTGACT GGACCCACTT TGGAGATGAA 
ATCCATGTAC TTCGCAATCA GTTGGTTCTG CTTCACAACC AGCTTTTGTA TGAAAGGTTC 
AAAAGGCAGC AGCATGCCCA ACGCAACCGC CGCCTCCTGC GCAAAGTTAT CAAGGTGACT 
TCACTGGAGG AACAGAATGC TGCTATGAAA AAACAGTTAA AGCTTCAGGA GGTGGACATG 
CATTCTATCA AACAGAGCCT CTTTCAGGAA CAGACGCGGT ACCGGCGGCT TCAGGAAGAG 
CGAGACACTG TGGTCACTCA ACTGCGCAGC CAGATCCGCC AGCTACAGCA TGACAGAGAT 
CTGTTCTATA ACCAAAGCCA GGAGCTGCAG ACCAAGCTAG AGGATTGCCG CACCACCATT 
GCAGATTTAC GTCTGGAGGT GAAACAAGCC AATAATAAAG TGTGCCATAC GGAGCTCCGT 
CTAAGCCAGG TTTCTCAAAA GCTCTCCAAC AGTGAGTCTG TACAGCATCA GATGGAGTTT 
CTGAACCGTC AGCTCCTGGT CCTGGGGGAG GTAAACGAGT TGTACGTGGA GCAGTTGCAG 
CAAAAGCAGA CAAACAGCAC AAAGGAAGTG GAGATGTTGC AGACTTCCTT TAAAAAGGAC 
CTGGAGAAGG CTCGATTGAA TGTTGTGCAA CTGAGTCAGA GGCTAGAGTG CACCCAGAAA 
CGAATAGTGG AGCTGGAAGC TCAGCTGGCC AAGAAAGAAT CCTTGTTTCA AGAACAGAAA 
AAATTCTTAG AGCATGTTAA GGGGAAGAGC AGAGGCCAGC TGCAAGCAGC CGAGAGCAGA 
TACCTCGCTC AGAAGAGGAT AAGCCAGGTC TTCGAAGTCA AAATCCTCAG TCTGTACAAC 
AGGTTGGAGA AGGAAGGGCC TCTGAAAAGT CAAAGTGAAG AGAAAGCGGA GACCGCAGAA 
GCCGCAGAAG AAAGGTGTGA CATTAACATT GCAAGTGTTT ACAGTCCAGA TGTTGGCATT 
CTAGAAGAAG TCAATGGTAG CGAATATAAC TTGCCTGCCA ACCGCACAAG CTCCTCCAGT 
AGCCAGAGCA GCAGCACCCT CTCCACCCCA GAGAAAGGCA CAAACTTACA GCTCAGCCGT 
CGGCAGTTAC TAGAGATCCC TTCCAGCGGC CACTTGACTA TCGGCTCCTA CCCCAACGCC 
AAAAGTTTCC TAGGCATCCG GGCCCGCGAG CTGTTCCGCA ACAAGAGCGA GAGCCACTGC 
GACGAGGAAA GCATCAGCAG CCTCTCGGAG ATTCTGAAAA CTGAACTCTG CCAGGAGCTT 
ATCCCAGAGA CGAATGCGGA GGGCTCCCCC CAAAATCAAG ATTGTGTGAG GAATCTTCAG 
ATTATGGACT ATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108699932 ( XM_018232635.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088124.1 Xenopus laevis hamartin-like (LOC108699932), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OAf43888
Clone ID Related Accession (Same CDS sequence)	XM_018232635.1
Accession Version	XM_018232635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	hamartin-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGCTGCTAT ATGCCAGGAG 
CTATCTCGGA TCACCATGCC TGACCCCGAG CCTACTGTGC TAAGAGGAGG ATTTGATTCC 
CCTTTCTCTC TTCGCTGTGA CAGTCAGCCC GGCTCTCAGA AAAAGCTTGC CTCCTATAGC 
TTTGGCCTCA TCCCCGAACC ATCTGCCACC GACAAGCTCC ACTACAGTGT GTCCCATGAC 
AACTCCAAAC AGGCGTTCAC TCCCATTGAA CCACCCAATG GCAATGAAAA TGCAACTGAC 
TCAGGGAAGC CGCCATCTAA CAACTCTTTG CCCAGCACTG CTGATATCTT CTCACCTCAG 
GTTCAAAGAA ACGTATCCTG TTTCAGCTTG GCTCCATACG AGCATCTCTT TGAACTGGCG 
TTGCCCAAAA CCGCTAACCT TTTTCTCTGC AATAAGACAG AGGAGCTGTG CAGAAGAGGC 
AAGGGAAGCC TTGAGGAGGG TGAAGAGGGT GCCTTCTCCA TTTCCCCAGC GGAAGTGCTT 
GACCGACTTA TCCAGTTGGG AGCAGATGCC CACACCAAAG AGCTGAGCAA CAGGTTGTCT 
TTACCCAGCA AAGCCGCTGA CTGGACCCAC TTTGGAGGTT CCCCACCAAC AGATGAAATC 
CATGTACTTC GCAATCAGTT GGTTCTGCTT CACAACCAGC TTTTGTATGA AAGGTTCAAA 
AGGCAGCAGC ATGCCCAACG CAACCGCCGC CTCCTGCGCA AAGTTATCAA GGTGACTTCA 
CTGGAGGAAC AGAATGCTGC TATGAAAAAA CAGTTAAAGC TTCAGGAGGT GGACATGCAT 
TCTATCAAAC AGAGCCTCTT TCAGGAACAG ACGCGGTACC GGCGGCTTCA GGAAGAGCGA 
GACACTGTGG TCACTCAACT GCGCAGCCAG ATCCGCCAGC TACAGCATGA CAGAGATCTG 
TTCTATAACC AAAGCCAGGA GCTGCAGACC AAGCTAGAGG ATTGCCGCAC CACCATTGCA 
GATTTACGTC TGGAGGTGAA ACAAGCCAAT AATAAAGTGT GCCATACGGA GCTCCGTCTA 
AGCCAGGTTT CTCAAAAGCT CTCCAACAGT GAGTCTGTAC AGCATCAGAT GGAGTTTCTG 
AACCGTCAGC TCCTGGTCCT GGGGGAGGTA AACGAGTTGT ACGTGGAGCA GTTGCAGCAA 
AAGCAGACAA ACAGCACAAA GGAAGTGGAG ATGTTGCAGA CTTCCTTTAA AAAGGACCTG 
GAGAAGGCTC GATTGAATGT TGTGCAACTG AGTCAGAGGC TAGAGTGCAC CCAGAAACGA 
ATAGTGGAGC TGGAAGCTCA GCTGGCCAAG AAAGAATCCT TGTTTCAAGA ACAGAAAAAA 
TTCTTAGAGC ATGTTAAGGG GAAGAGCAGA GGCCAGCTGC AAGCAGCCGA GAGCAGATAC 
CTCGCTCAGA AGAGGATAAG CCAGGTCTTC GAAGTCAAAA TCCTCAGTCT GTACAACAGG 
TTGGAGAAGG AAGGGCCTCT GAAAAGTCAA AGTGAAGAGA AAGCGGAGAC CGCAGAAGCC 
GCAGAAGAAA GGTGTGACAT TAACATTGCA AGTGTTTACA GTCCAGATGT TGGCATTCTA 
GAAGAAGTCA ATGGTAGCGA ATATAACTTG CCTGCCAACC GCACAAGCTC CTCCAGTAGC 
CAGAGCAGCA GCACCCTCTC CACCCCAGAG AAAGGCACAA ACTTACAGCT CAGCCGTCGG 
CAGTTACTAG AGATCCCTTC CAGCGGCCAC TTGACTATCG GCTCCTACCC CAACGCCAAA 
AGTTTCCTAG GCATCCGGGC CCGCGAGCTG TTCCGCAACA AGAGCGAGAG CCACTGCGAC 
GAGGAAAGCA TCAGCAGCCT CTCGGAGATT CTGAAAACTG AACTCTGCCA GGAGCTTATC 
CCAGAGACGA ATGCGGAGGG CTCCCCCCAA AATCAAGATT GTGTGAGGAA TCTTCAGATT 
ATGGACTATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088124.1
CDS	443..3757
Translation	MAQQTTVWEHLYLLESSDLQQLDQIHTLIRDHLIADRSPILVNNLVDHYVESGSEQAMLILSMVQEPHDKHLLDKINEYLVKAASRLPTLTLLGHVIRRQPSWKHKLSQAPVLQSLLKCLKVDNDVVVLTTGVLVLITVLPMIPQSGKQHLYEFFDIFGRLATWCLKNPGRVAEMCLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEIVKPMMEHVRIHPELVTGSKDHELDPSRWKRLETHDIVVECAKISLDPSEASYEEGYSPLSNRLRQRQHDTTSSPYTEIRGSNASTPRTCTQSAPPHTASIELLQDSIWSPSTICGMTTPPNSPANVLVDASHASSRPPSKVLYTPGGKATPLGTPATSPPPTSNSEECTPASGASLTPHKKNESSDPGRPATNRQLLSKGEKSTDESPKTQKTVYLSELPDIISDFAADHNSTEEAAICQELSRITMPDPEPTVLRGGFDSPFSLRCDSQPGSQKKLASYSFGLIPEPSATDKLHYSVSHDNSKQAFTPIEPPNGNENATDSGKPPSNNSLPSTADIFSPQVQRNVSCFSLAPYEHLFELALPKTANLFLCNKTEELCRRGKGSLEEGEEGAFSISPAEVLDRLIQLGADAHTKELSNRLSLPSKAADWTHFGGSPPTDEIHVLRNQLVLLHNQLLYERFKRQQHAQRNRRLLRKVIKVTSLEEQNAAMKKQLKLQEVDMHSIKQSLFQEQTRYRRLQEERDTVVTQLRSQIRQLQHDRDLFYNQSQELQTKLEDCRTTIADLRLEVKQANNKVCHTELRLSQVSQKLSNSESVQHQMEFLNRQLLVLGEVNELYVEQLQQKQTNSTKEVEMLQTSFKKDLEKARLNVVQLSQRLECTQKRIVELEAQLAKKESLFQEQKKFLEHVKGKSRGQLQAAESRYLAQKRISQVFEVKILSLYNRLEKEGPLKSQSEEKAETAEAAEERCDINIASVYSPDVGILEEVNGSEYNLPANRTSSSSSQSSSTLSTPEKGTNLQLSRRQLLEIPSSGHLTIGSYPNAKSFLGIRARELFRNKSESHCDEESISSLSEILKTELCQELIPETNAEGSPQNQDCVRNLQIMDYD

Target ORF information:

RefSeq Version	XM_018232635.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis hamartin-like (LOC108699932), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232635.1

ORF Insert Sequence:

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGCTGCTAT ATGCCAGGAG 
CTATCTCGGA TCACCATGCC TGACCCCGAG CCTACTGTGC TAAGAGGAGG ATTTGATTCC 
CCTTTCTCTC TTCGCTGTGA CAGTCAGCCC GGCTCTCAGA AAAAGCTTGC CTCCTATAGC 
TTTGGCCTCA TCCCCGAACC ATCTGCCACC GACAAGCTCC ACTACAGTGT GTCCCATGAC 
AACTCCAAAC AGGCGTTCAC TCCCATTGAA CCACCCAATG GCAATGAAAA TGCAACTGAC 
TCAGGGAAGC CGCCATCTAA CAACTCTTTG CCCAGCACTG CTGATATCTT CTCACCTCAG 
GTTCAAAGAA ACGTATCCTG TTTCAGCTTG GCTCCATACG AGCATCTCTT TGAACTGGCG 
TTGCCCAAAA CCGCTAACCT TTTTCTCTGC AATAAGACAG AGGAGCTGTG CAGAAGAGGC 
AAGGGAAGCC TTGAGGAGGG TGAAGAGGGT GCCTTCTCCA TTTCCCCAGC GGAAGTGCTT 
GACCGACTTA TCCAGTTGGG AGCAGATGCC CACACCAAAG AGCTGAGCAA CAGGTTGTCT 
TTACCCAGCA AAGCCGCTGA CTGGACCCAC TTTGGAGGTT CCCCACCAAC AGATGAAATC 
CATGTACTTC GCAATCAGTT GGTTCTGCTT CACAACCAGC TTTTGTATGA AAGGTTCAAA 
AGGCAGCAGC ATGCCCAACG CAACCGCCGC CTCCTGCGCA AAGTTATCAA GGTGACTTCA 
CTGGAGGAAC AGAATGCTGC TATGAAAAAA CAGTTAAAGC TTCAGGAGGT GGACATGCAT 
TCTATCAAAC AGAGCCTCTT TCAGGAACAG ACGCGGTACC GGCGGCTTCA GGAAGAGCGA 
GACACTGTGG TCACTCAACT GCGCAGCCAG ATCCGCCAGC TACAGCATGA CAGAGATCTG 
TTCTATAACC AAAGCCAGGA GCTGCAGACC AAGCTAGAGG ATTGCCGCAC CACCATTGCA 
GATTTACGTC TGGAGGTGAA ACAAGCCAAT AATAAAGTGT GCCATACGGA GCTCCGTCTA 
AGCCAGGTTT CTCAAAAGCT CTCCAACAGT GAGTCTGTAC AGCATCAGAT GGAGTTTCTG 
AACCGTCAGC TCCTGGTCCT GGGGGAGGTA AACGAGTTGT ACGTGGAGCA GTTGCAGCAA 
AAGCAGACAA ACAGCACAAA GGAAGTGGAG ATGTTGCAGA CTTCCTTTAA AAAGGACCTG 
GAGAAGGCTC GATTGAATGT TGTGCAACTG AGTCAGAGGC TAGAGTGCAC CCAGAAACGA 
ATAGTGGAGC TGGAAGCTCA GCTGGCCAAG AAAGAATCCT TGTTTCAAGA ACAGAAAAAA 
TTCTTAGAGC ATGTTAAGGG GAAGAGCAGA GGCCAGCTGC AAGCAGCCGA GAGCAGATAC 
CTCGCTCAGA AGAGGATAAG CCAGGTCTTC GAAGTCAAAA TCCTCAGTCT GTACAACAGG 
TTGGAGAAGG AAGGGCCTCT GAAAAGTCAA AGTGAAGAGA AAGCGGAGAC CGCAGAAGCC 
GCAGAAGAAA GGTGTGACAT TAACATTGCA AGTGTTTACA GTCCAGATGT TGGCATTCTA 
GAAGAAGTCA ATGGTAGCGA ATATAACTTG CCTGCCAACC GCACAAGCTC CTCCAGTAGC 
CAGAGCAGCA GCACCCTCTC CACCCCAGAG AAAGGCACAA ACTTACAGCT CAGCCGTCGG 
CAGTTACTAG AGATCCCTTC CAGCGGCCAC TTGACTATCG GCTCCTACCC CAACGCCAAA 
AGTTTCCTAG GCATCCGGGC CCGCGAGCTG TTCCGCAACA AGAGCGAGAG CCACTGCGAC 
GAGGAAAGCA TCAGCAGCCT CTCGGAGATT CTGAAAACTG AACTCTGCCA GGAGCTTATC 
CCAGAGACGA ATGCGGAGGG CTCCCCCCAA AATCAAGATT GTGTGAGGAA TCTTCAGATT 
ATGGACTATG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108699932 ( XM_018232636.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088125.1 Xenopus laevis hamartin-like (LOC108699932), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OAf43889
Clone ID Related Accession (Same CDS sequence)	XM_018232636.1
Accession Version	XM_018232636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	hamartin-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GACAGAGGAG CTGTGCAGAA GAGGCAAGGG AAGCCTTGAG 
GAGGGTGAAG AGGGTGCCTT CTCCATTTCC CCAGCGGAAG TGCTTGACCG ACTTATCCAG 
TTGGGAGCAG ATGCCCACAC CAAAGAGCTG AGCAACAGGT TGTCTTTACC CAGCAAAGCC 
GCTGACTGGA CCCACTTTGG AGGTTCCCCA CCAACAGATG AAATCCATGT ACTTCGCAAT 
CAGTTGGTTC TGCTTCACAA CCAGCTTTTG TATGAAAGGT TCAAAAGGCA GCAGCATGCC 
CAACGCAACC GCCGCCTCCT GCGCAAAGTT ATCAAGGTGA CTTCACTGGA GGAACAGAAT 
GCTGCTATGA AAAAACAGTT AAAGCTTCAG GAGGTGGACA TGCATTCTAT CAAACAGAGC 
CTCTTTCAGG AACAGACGCG GTACCGGCGG CTTCAGGAAG AGCGAGACAC TGTGGTCACT 
CAACTGCGCA GCCAGATCCG CCAGCTACAG CATGACAGAG ATCTGTTCTA TAACCAAAGC 
CAGGAGCTGC AGACCAAGCT AGAGGATTGC CGCACCACCA TTGCAGATTT ACGTCTGGAG 
GTGAAACAAG CCAATAATAA AGTGTGCCAT ACGGAGCTCC GTCTAAGCCA GGTTTCTCAA 
AAGCTCTCCA ACAGTGAGTC TGTACAGCAT CAGATGGAGT TTCTGAACCG TCAGCTCCTG 
GTCCTGGGGG AGGTAAACGA GTTGTACGTG GAGCAGTTGC AGCAAAAGCA GACAAACAGC 
ACAAAGGAAG TGGAGATGTT GCAGACTTCC TTTAAAAAGG ACCTGGAGAA GGCTCGATTG 
AATGTTGTGC AACTGAGTCA GAGGCTAGAG TGCACCCAGA AACGAATAGT GGAGCTGGAA 
GCTCAGCTGG CCAAGAAAGA ATCCTTGTTT CAAGAACAGA AAAAATTCTT AGAGCATGTT 
AAGGGGAAGA GCAGAGGCCA GCTGCAAGCA GCCGAGAGCA GATACCTCGC TCAGAAGAGG 
ATAAGCCAGG TCTTCGAAGT CAAAATCCTC AGTCTGTACA ACAGGTTGGA GAAGGAAGGG 
CCTCTGAAAA GTCAAAGTGA AGAGAAAGCG GAGACCGCAG AAGCCGCAGA AGAAAGGTGT 
GACATTAACA TTGCAAGTGT TTACAGTCCA GATGTTGGCA TTCTAGAAGA AGTCAATGGT 
AGCGAATATA ACTTGCCTGC CAACCGCACA AGCTCCTCCA GTAGCCAGAG CAGCAGCACC 
CTCTCCACCC CAGAGAAAGG CACAAACTTA CAGCTCAGCC GTCGGCAGTT ACTAGAGATC 
CCTTCCAGCG GCCACTTGAC TATCGGCTCC TACCCCAACG CCAAAAGTTT CCTAGGCATC 
CGGGCCCGCG AGCTGTTCCG CAACAAGAGC GAGAGCCACT GCGACGAGGA AAGCATCAGC 
AGCCTCTCGG AGATTCTGAA AACTGAACTC TGCCAGGAGC TTATCCCAGA GACGAATGCG 
GAGGGCTCCC CCCAAAATCA AGATTGTGTG AGGAATCTTC AGATTATGGA CTATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088125.1
CDS	443..3682
Translation	MAQQTTVWEHLYLLESSDLQQLDQIHTLIRDHLIADRSPILVNNLVDHYVESGSEQAMLILSMVQEPHDKHLLDKINEYLVKAASRLPTLTLLGHVIRRQPSWKHKLSQAPVLQSLLKCLKVDNDVVVLTTGVLVLITVLPMIPQSGKQHLYEFFDIFGRLATWCLKNPGRVAEMCLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEIVKPMMEHVRIHPELVTGSKDHELDPSRWKRLETHDIVVECAKISLDPSEASYEEGYSPLSNRLRQRQHDTTSSPYTEIRGSNASTPRTCTQSAPPHTASIELLQDSIWSPSTICGMTTPPNSPANVLVDASHASSRPPSKVLYTPGGKATPLGTPATSPPPTSNSEECTPASGASLTPHKKNESSDPGRPATNRQLLSKGEKSTDESPKTQKTVYLSELPDIISDFAADHNSTEEELKKNGHAAICQELSRITMPDPEPTVLRGGFDSPFSLRCDSQPGSQKKLASYSFGLIPEPSATDKLHYSVSHDNSKQAFTPIEPPNGNENATDSGKPPSNNSLPSTADIFSPQTEELCRRGKGSLEEGEEGAFSISPAEVLDRLIQLGADAHTKELSNRLSLPSKAADWTHFGGSPPTDEIHVLRNQLVLLHNQLLYERFKRQQHAQRNRRLLRKVIKVTSLEEQNAAMKKQLKLQEVDMHSIKQSLFQEQTRYRRLQEERDTVVTQLRSQIRQLQHDRDLFYNQSQELQTKLEDCRTTIADLRLEVKQANNKVCHTELRLSQVSQKLSNSESVQHQMEFLNRQLLVLGEVNELYVEQLQQKQTNSTKEVEMLQTSFKKDLEKARLNVVQLSQRLECTQKRIVELEAQLAKKESLFQEQKKFLEHVKGKSRGQLQAAESRYLAQKRISQVFEVKILSLYNRLEKEGPLKSQSEEKAETAEAAEERCDINIASVYSPDVGILEEVNGSEYNLPANRTSSSSSQSSSTLSTPEKGTNLQLSRRQLLEIPSSGHLTIGSYPNAKSFLGIRARELFRNKSESHCDEESISSLSEILKTELCQELIPETNAEGSPQNQDCVRNLQIMDYD

Target ORF information:

RefSeq Version	XM_018232636.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis hamartin-like (LOC108699932), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232636.1

ORF Insert Sequence:

ATGGCCCAGC AAACCACTGT GTGGGAGCAT CTGTATCTGC TGGAATCTTC AGACCTGCAG 
CAACTGGATC AGATCCATAC ACTTATCAGA GACCACCTTA TCGCAGATCG CTCCCCCATT 
CTGGTCAACA ACCTGGTGGA TCATTATGTG GAAAGTGGTT CGGAACAAGC AATGCTGATC 
CTTTCAATGG TACAAGAACC TCACGACAAG CACCTTTTGG ACAAGATAAA TGAGTATTTA 
GTTAAAGCTG CTAGCCGCTT ACCAACGCTA ACACTATTAG GCCATGTGAT CCGTCGCCAG 
CCATCTTGGA AGCACAAACT CTCCCAAGCA CCCGTCCTTC AGTCCTTACT CAAGTGTCTC 
AAGGTAGATA ATGATGTTGT CGTCCTCACC ACGGGAGTCC TAGTTTTAAT TACTGTGCTG 
CCAATGATTC CGCAGTCTGG CAAACAGCAT CTCTACGAGT TCTTTGACAT ATTTGGACGC 
CTGGCAACAT GGTGCCTAAA GAACCCTGGT CGTGTAGCAG AGATGTGTCT CGTCCATCTT 
CACGCCAGCG TGTATGCCCT GTTCCATCGG CTTTATGGGA TGTATCCATG CAATTTTGTA 
TCGTTCCTGC GCTCCCATTA CAGTATGAAG GAAAACCTGG AGACCTTTGA GGAAATAGTC 
AAGCCAATGA TGGAGCATGT GCGGATTCAC CCAGAGCTTG TAACTGGCTC AAAAGACCAT 
GAACTGGACC CAAGCAGGTG GAAGAGGTTG GAAACACATG ACATTGTTGT AGAATGTGCC 
AAAATATCTC TGGACCCCAG TGAAGCTTCC TATGAAGAAG GATATTCCCC CCTTTCCAAT 
CGCCTGAGGC AGAGGCAACA TGACACCACA TCCAGCCCCT ATACTGAAAT CAGAGGAAGC 
AATGCCTCCA CTCCTCGAAC ATGTACGCAG AGTGCACCAC CTCACACTGC AAGCATTGAG 
CTACTGCAGG ACAGCATCTG GAGTCCGTCC ACCATTTGTG GAATGACCAC CCCGCCTAAC 
TCGCCTGCCA ATGTGCTTGT AGATGCATCT CATGCCTCCT CTCGTCCCCC TAGCAAAGTT 
CTGTACACGC CTGGAGGCAA AGCAACACCT CTGGGAACAC CAGCAACCTC CCCACCTCCA 
ACCAGCAACT CTGAGGAATG TACCCCAGCC TCCGGTGCTT CACTGACACC CCATAAGAAG 
AATGAATCCT CTGATCCAGG AAGGCCTGCA ACAAACAGGC AGCTGCTCAG CAAAGGAGAA 
AAAAGCACTG ACGAATCACC AAAGACACAG AAGACTGTGT ATTTAAGTGA GCTTCCGGAT 
ATCATTAGTG ATTTTGCGGC TGACCACAAT AGCACTGAGG AAGAACTGAA GAAAAATGGT 
CACGCTGCTA TATGCCAGGA GCTATCTCGG ATCACCATGC CTGACCCCGA GCCTACTGTG 
CTAAGAGGAG GATTTGATTC CCCTTTCTCT CTTCGCTGTG ACAGTCAGCC CGGCTCTCAG 
AAAAAGCTTG CCTCCTATAG CTTTGGCCTC ATCCCCGAAC CATCTGCCAC CGACAAGCTC 
CACTACAGTG TGTCCCATGA CAACTCCAAA CAGGCGTTCA CTCCCATTGA ACCACCCAAT 
GGCAATGAAA ATGCAACTGA CTCAGGGAAG CCGCCATCTA ACAACTCTTT GCCCAGCACT 
GCTGATATCT TCTCACCTCA GACAGAGGAG CTGTGCAGAA GAGGCAAGGG AAGCCTTGAG 
GAGGGTGAAG AGGGTGCCTT CTCCATTTCC CCAGCGGAAG TGCTTGACCG ACTTATCCAG 
TTGGGAGCAG ATGCCCACAC CAAAGAGCTG AGCAACAGGT TGTCTTTACC CAGCAAAGCC 
GCTGACTGGA CCCACTTTGG AGGTTCCCCA CCAACAGATG AAATCCATGT ACTTCGCAAT 
CAGTTGGTTC TGCTTCACAA CCAGCTTTTG TATGAAAGGT TCAAAAGGCA GCAGCATGCC 
CAACGCAACC GCCGCCTCCT GCGCAAAGTT ATCAAGGTGA CTTCACTGGA GGAACAGAAT 
GCTGCTATGA AAAAACAGTT AAAGCTTCAG GAGGTGGACA TGCATTCTAT CAAACAGAGC 
CTCTTTCAGG AACAGACGCG GTACCGGCGG CTTCAGGAAG AGCGAGACAC TGTGGTCACT 
CAACTGCGCA GCCAGATCCG CCAGCTACAG CATGACAGAG ATCTGTTCTA TAACCAAAGC 
CAGGAGCTGC AGACCAAGCT AGAGGATTGC CGCACCACCA TTGCAGATTT ACGTCTGGAG 
GTGAAACAAG CCAATAATAA AGTGTGCCAT ACGGAGCTCC GTCTAAGCCA GGTTTCTCAA 
AAGCTCTCCA ACAGTGAGTC TGTACAGCAT CAGATGGAGT TTCTGAACCG TCAGCTCCTG 
GTCCTGGGGG AGGTAAACGA GTTGTACGTG GAGCAGTTGC AGCAAAAGCA GACAAACAGC 
ACAAAGGAAG TGGAGATGTT GCAGACTTCC TTTAAAAAGG ACCTGGAGAA GGCTCGATTG 
AATGTTGTGC AACTGAGTCA GAGGCTAGAG TGCACCCAGA AACGAATAGT GGAGCTGGAA 
GCTCAGCTGG CCAAGAAAGA ATCCTTGTTT CAAGAACAGA AAAAATTCTT AGAGCATGTT 
AAGGGGAAGA GCAGAGGCCA GCTGCAAGCA GCCGAGAGCA GATACCTCGC TCAGAAGAGG 
ATAAGCCAGG TCTTCGAAGT CAAAATCCTC AGTCTGTACA ACAGGTTGGA GAAGGAAGGG 
CCTCTGAAAA GTCAAAGTGA AGAGAAAGCG GAGACCGCAG AAGCCGCAGA AGAAAGGTGT 
GACATTAACA TTGCAAGTGT TTACAGTCCA GATGTTGGCA TTCTAGAAGA AGTCAATGGT 
AGCGAATATA ACTTGCCTGC CAACCGCACA AGCTCCTCCA GTAGCCAGAG CAGCAGCACC 
CTCTCCACCC CAGAGAAAGG CACAAACTTA CAGCTCAGCC GTCGGCAGTT ACTAGAGATC 
CCTTCCAGCG GCCACTTGAC TATCGGCTCC TACCCCAACG CCAAAAGTTT CCTAGGCATC 
CGGGCCCGCG AGCTGTTCCG CAACAAGAGC GAGAGCCACT GCGACGAGGA AAGCATCAGC 
AGCCTCTCGG AGATTCTGAA AACTGAACTC TGCCAGGAGC TTATCCCAGA GACGAATGCG 
GAGGGCTCCC CCCAAAATCA AGATTGTGTG AGGAATCTTC AGATTATGGA CTATGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC108699932 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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