LOC100266796 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100266796
Entrez Gene ID	100266796
Full Name	ubiquitin carboxyl-terminal hydrolase 12
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002277664.3	XP_002277700.1	ubiquitin carboxyl-terminal hydrolase 12 isoform X1
XM_010653329.2	XP_010651631.1	ubiquitin carboxyl-terminal hydrolase 12 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100266796 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100266796 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa06551	XM_002277664.3 Latest version!	Vitis vinifera ubiquitin carboxyl-terminal hydrolase 12 (LOC100266796), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OVa33145	XM_010653329.2 Latest version!	Vitis vinifera ubiquitin carboxyl-terminal hydrolase 12 (LOC100266796), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100266796 ( XM_002277664.3 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_002277700.1 Vitis vinifera ubiquitin carboxyl-terminal hydrolase 12 (LOC100266796), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OVa06551
Clone ID Related Accession (Same CDS sequence)	XM_002277664.3
Accession Version	XM_002277664.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479916800000
Organism	Vitis vinifera(wine grape)
Product	ubiquitin carboxyl-terminal hydrolase 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012012.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 25, 2016 this sequence version replaced XM_002277664.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTGA TGACTCCTGC TTCTATTGAA CGAGAGGACG AGGAGATGCT GGTTCCCCAC 
ACAGATTTGG CCGACGGCCA TCAACCTATG GAAGTTGTTG CACAAGAGGA AACTACTAGT 
ACTGTGGAAA ACCAGCCAGT TGAGGATCCT CCTACATCAA GGTTCACATG GAGAATTGAG 
AACTTTTCTA GGTTGAATAC AAAGAAGCAC TATTCCGAAA ATTTCATTGT GGGCGGCTAT 
AAGTGGCGGG TGCTTATTTT TCCAAAGGGA AACAATGTTG AACATTTGTC TATGTACTTG 
GATGTTGCGG ATTCATCAAG TTTGCCCTAT GGGTGGAGTA GATATGCACA ATTCAGCTTG 
GCTGTGGTAA ATCAAATTCA TAACAAATAT ACAGTGAGAA AAGACACTCA ACACCAGTTT 
AATGCACGAG AAAGTGATTG GGGTTTCACT TCTTTTATGC CTCTTAGTGA ACTATACGAT 
CCAGGTAGGG GTTTTCTTGT GAGTGATACA TGTATAGTTG AAGCAGAGGT TGCTGTCCGC 
AGAGTTGTGG ATTACTGGAC ATATGACTCA AAAAAGGAAA CTGGTTATGT TGGACTTAAG 
AACCAAGGAG CAACTTGTTA CATGAACTCT CTCCTCCAAA CTCTCTATCA TATTCCCTAC 
TTTAGAAAGG CTGTGTACCA TATGCCAACG ACTGAGAATG ATATGCCATC TGGAAGCATT 
CCTTTGGCTT TGCAGAGTTT ATTCTATAAG CTTCAATACA GTGACAGCAG CGTTGCAACG 
AAAGAGTTGA CAAAATCGTT TGGTTGGGAC ACATATGATT CTTTCTTGCA GCATGATGTG 
CAAGAACTCA ATAGGGTCCT CTGTGAAAAG CTTGAAGACA AAATGAAGGG AACTGTTGTG 
GAGGGCACTA TACAGCAGTT ATTTGAAGGG CACCATATGA ACTACATTGA ATGCATCAAT 
GTGGACTACA AATCTACAAG GAAGGAGTCA TTTTATGACC TTCAGCTGGA TGTCAAAGGT 
TGTCATGATG TTTATGCTTC TTTTGATAAG TACGTAGAAG TTGAGCGCCT AGAAGGGGAT 
AACAAATATC ATGCTGAGCA CCATGGATTG CAGGATGCTA GGAAGGGTGT CTTATTCATT 
GACTTCCCTC CTGTCCTTCA ACTTCAGCTG AAGCGATTTG AATATGATTT TATGCGGGAC 
ACTATGGTAA AGATAAATGA TCGCTATGAG TTCCCTCTGC AACTTGATCT TGATCGAGAG 
AATGGCAAAT ATTTATCTCC TGATGCCAAT AGGACTGTTC GCAACCTTTA CGCCCTTCAC 
AGTGTTCTAG TTCACAGTGG CGGAGTGCAT GGTGGACATT ATTATGCCTT TATAAGACCC 
ACCCTTTCTG ATCAGTGGTA TAAATTTGAT GATGAAAGGG TGACAAAAGA AGACGTGAAG 
AGGGCTTTGG AGGAGCAATA TGGTGGTGAA GAAGAGCTGC CCCAGACAAA TCCTGGATTG 
AATAACACCC CCTTTAAATT TACGAAGTAT TCAAATGCAT ACATGCTGGT GTATATACGT 
GAAAGTGACA AGGACAAAAT AATTTGCAAT GTGGATGAGA AGGACATTGC TGAACATCTC 
CGGGAGAGGT TGAAGAAAGA ACAAGAAGAA AAAGAGCACA AGAAAAAGGA AAAAGCCGAG 
TCACATCTTT ATACCATTAT TAAGGTTGCA CGAGATGATG ACCTTGTAGA ACATATTGGA 
AGGGATATAT ATTTTGATCT TGTGGATCAT GACAAAGTTA GAAGTTTTCG TATCCAGAAG 
CAGATGCCTT TCAACTTTTT CAAGGAGGAG GTAGCTAAAG AGTTTGGTAT ACCAATTCAA 
TTTCAGCGTT TTTGGCTGTG GGCAAAGCGC CAAAACCACA CATATCGTCC TAATCGGCCA 
TTGACACATC TAGAAGAACA ACAATCTGTT GGACAGTTAA GGGAGATATC AAATAAGGTT 
CAAAATGCAG AACTAAAGTT GTTCTTGGAA GTAAATCTTG GGCCGGATTT ACACCCAAAC 
CCTCCACCTG AGAAGACAAA GGATGATATT TTGCTTTTCT TCAAGCTTTA TGATCCTGAG 
AAGGAAGAGC TAAATTATGT TGGAAGGCTT TTTGTGAAGA GTACTGGTAA GCCTGTAGAA 
ATTTTGTCAA AACTGAATGA AATGGTTGGT TATGCTCCTG ATGAAGAGAT TGAACTCTAT 
GAGGAAATCA AGTTTGATCC TAGTGTCATG TGTGAACCCA TTGATAAGAA ATTCACATTC 
CGAGCTAGCC AGCTTGAAGA TGGTGATATC ATTTGCTTTC AAAAAACACC TCCAATTGAA 
AGTGGTGAAT CATTCCGCTA TCCAGATGTT CCTTCATTTT TAGAATACGT GCACAATCGC 
CAAGTTGTTC ATTTTCGGTC CCTAGAAAAA CCCAAGGAAG ATGATTTTTG TCTTGAGATG 
TCAAAGCTTT TTACTTATGA TGACGTGGTA GAAAGAGTAG CTCGTCAGCT TGGTTTGGAT 
GATCCATCCA AAATCAGGCT CACATCTCAC AACTGTTACT CTCAGCAACC AAAACCCCAG 
CCAATTAAGT ACCGTGGGGT GGACCATTTG TCTGACATGC TGGTTCACTA CAATCTGATT 
TCGGATGTAT TGTACTATGA AGTATTGGAC ATCCCTCTTC CAGAATTACA AGGTTTAAAA 
ACTTTGAAAG TTGCTTTTCA TCATGCTGAA AAGGAGGAAG TTGTTAGTCA TAGTATTAGA 
CTGCCAAAAC AGAGTACAGT AGGGGATGTA ATTAATGCTC TTAAAACAAA GGTTGAGCTG 
TCTCATCCTA ATGCTGAAGT TAGGTTGCTT GAAGTTTTCT ATCACAAGAT CTATAAGGTT 
TTTCCTTCAA ATGAAAAAAT TGAGAACATT AATGATCAAT ACTGGACACT GCGTGCTGAA 
GAGATTCCTG AAGAAGAGAA AAATCTTGGA CCACAGGATC GCTTGATTCA TGTATACCAC 
TTTACAAAAG ACACAGCTCA GAACCAAATG CAAATTCAGA ATTTCGGGGA GCCTTTTTTC 
TTGGTCATCC ATGAGGGTGA AACTCTGGCT GAAGTTAAGG TGCGGATACA GAAGAAGCTG 
CTGGTTCCAG AGGAAGAATT TGCTAAGTGG AGGTTTGCCT TTCTTTCACT AGGTCGCCCA 
GAGTACCTTC AAGACTCTGA CATTGTCTCC AGTCGTTTTC AGAGAAGAGA TGTTTATGGT 
GCTTGGGAGC AGTATCTTGG ACTAGAGCAT TCTGACACTG CTCCTAAAAG GGCTTATGCA 
GCTAATCAGA ACCGTCACAC ATTTGAGAAG CCGGTAAAAA TCTACAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002277700.1
CDS	175..3525
Translation	MTVMTPASIEREDEEMLVPHTDLADGHQPMEVVAQEETTSTVENQPVEDPPTSRFTWRIENFSRLNTKKHYSENFIVGGYKWRVLIFPKGNNVEHLSMYLDVADSSSLPYGWSRYAQFSLAVVNQIHNKYTVRKDTQHQFNARESDWGFTSFMPLSELYDPGRGFLVSDTCIVEAEVAVRRVVDYWTYDSKKETGYVGLKNQGATCYMNSLLQTLYHIPYFRKAVYHMPTTENDMPSGSIPLALQSLFYKLQYSDSSVATKELTKSFGWDTYDSFLQHDVQELNRVLCEKLEDKMKGTVVEGTIQQLFEGHHMNYIECINVDYKSTRKESFYDLQLDVKGCHDVYASFDKYVEVERLEGDNKYHAEHHGLQDARKGVLFIDFPPVLQLQLKRFEYDFMRDTMVKINDRYEFPLQLDLDRENGKYLSPDANRTVRNLYALHSVLVHSGGVHGGHYYAFIRPTLSDQWYKFDDERVTKEDVKRALEEQYGGEEELPQTNPGLNNTPFKFTKYSNAYMLVYIRESDKDKIICNVDEKDIAEHLRERLKKEQEEKEHKKKEKAESHLYTIIKVARDDDLVEHIGRDIYFDLVDHDKVRSFRIQKQMPFNFFKEEVAKEFGIPIQFQRFWLWAKRQNHTYRPNRPLTHLEEQQSVGQLREISNKVQNAELKLFLEVNLGPDLHPNPPPEKTKDDILLFFKLYDPEKEELNYVGRLFVKSTGKPVEILSKLNEMVGYAPDEEIELYEEIKFDPSVMCEPIDKKFTFRASQLEDGDIICFQKTPPIESGESFRYPDVPSFLEYVHNRQVVHFRSLEKPKEDDFCLEMSKLFTYDDVVERVARQLGLDDPSKIRLTSHNCYSQQPKPQPIKYRGVDHLSDMLVHYNLISDVLYYEVLDIPLPELQGLKTLKVAFHHAEKEEVVSHSIRLPKQSTVGDVINALKTKVELSHPNAEVRLLEVFYHKIYKVFPSNEKIENINDQYWTLRAEEIPEEEKNLGPQDRLIHVYHFTKDTAQNQMQIQNFGEPFFLVIHEGETLAEVKVRIQKKLLVPEEEFAKWRFAFLSLGRPEYLQDSDIVSSRFQRRDVYGAWEQYLGLEHSDTAPKRAYAANQNRHTFEKPVKIYN

Target ORF information:

RefSeq Version	XM_002277664.3
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera ubiquitin carboxyl-terminal hydrolase 12 (LOC100266796), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002277664.3

ORF Insert Sequence:

ATGACTGTGA TGACTCCTGC TTCTATTGAA CGAGAGGACG AGGAGATGCT GGTTCCCCAC 
ACAGATTTGG CCGACGGCCA TCAACCTATG GAAGTTGTTG CACAAGAGGA AACTACTAGT 
ACTGTGGAAA ACCAGCCAGT TGAGGATCCT CCTACATCAA GGTTCACATG GAGAATTGAG 
AACTTTTCTA GGTTGAATAC AAAGAAGCAC TATTCCGAAA ATTTCATTGT GGGCGGCTAT 
AAGTGGCGGG TGCTTATTTT TCCAAAGGGA AACAATGTTG AACATTTGTC TATGTACTTG 
GATGTTGCGG ATTCATCAAG TTTGCCCTAT GGGTGGAGTA GATATGCACA ATTCAGCTTG 
GCTGTGGTAA ATCAAATTCA TAACAAATAT ACAGTGAGAA AAGACACTCA ACACCAGTTT 
AATGCACGAG AAAGTGATTG GGGTTTCACT TCTTTTATGC CTCTTAGTGA ACTATACGAT 
CCAGGTAGGG GTTTTCTTGT GAGTGATACA TGTATAGTTG AAGCAGAGGT TGCTGTCCGC 
AGAGTTGTGG ATTACTGGAC ATATGACTCA AAAAAGGAAA CTGGTTATGT TGGACTTAAG 
AACCAAGGAG CAACTTGTTA CATGAACTCT CTCCTCCAAA CTCTCTATCA TATTCCCTAC 
TTTAGAAAGG CTGTGTACCA TATGCCAACG ACTGAGAATG ATATGCCATC TGGAAGCATT 
CCTTTGGCTT TGCAGAGTTT ATTCTATAAG CTTCAATACA GTGACAGCAG CGTTGCAACG 
AAAGAGTTGA CAAAATCGTT TGGTTGGGAC ACATATGATT CTTTCTTGCA GCATGATGTG 
CAAGAACTCA ATAGGGTCCT CTGTGAAAAG CTTGAAGACA AAATGAAGGG AACTGTTGTG 
GAGGGCACTA TACAGCAGTT ATTTGAAGGG CACCATATGA ACTACATTGA ATGCATCAAT 
GTGGACTACA AATCTACAAG GAAGGAGTCA TTTTATGACC TTCAGCTGGA TGTCAAAGGT 
TGTCATGATG TTTATGCTTC TTTTGATAAG TACGTAGAAG TTGAGCGCCT AGAAGGGGAT 
AACAAATATC ATGCTGAGCA CCATGGATTG CAGGATGCTA GGAAGGGTGT CTTATTCATT 
GACTTCCCTC CTGTCCTTCA ACTTCAGCTG AAGCGATTTG AATATGATTT TATGCGGGAC 
ACTATGGTAA AGATAAATGA TCGCTATGAG TTCCCTCTGC AACTTGATCT TGATCGAGAG 
AATGGCAAAT ATTTATCTCC TGATGCCAAT AGGACTGTTC GCAACCTTTA CGCCCTTCAC 
AGTGTTCTAG TTCACAGTGG CGGAGTGCAT GGTGGACATT ATTATGCCTT TATAAGACCC 
ACCCTTTCTG ATCAGTGGTA TAAATTTGAT GATGAAAGGG TGACAAAAGA AGACGTGAAG 
AGGGCTTTGG AGGAGCAATA TGGTGGTGAA GAAGAGCTGC CCCAGACAAA TCCTGGATTG 
AATAACACCC CCTTTAAATT TACGAAGTAT TCAAATGCAT ACATGCTGGT GTATATACGT 
GAAAGTGACA AGGACAAAAT AATTTGCAAT GTGGATGAGA AGGACATTGC TGAACATCTC 
CGGGAGAGGT TGAAGAAAGA ACAAGAAGAA AAAGAGCACA AGAAAAAGGA AAAAGCCGAG 
TCACATCTTT ATACCATTAT TAAGGTTGCA CGAGATGATG ACCTTGTAGA ACATATTGGA 
AGGGATATAT ATTTTGATCT TGTGGATCAT GACAAAGTTA GAAGTTTTCG TATCCAGAAG 
CAGATGCCTT TCAACTTTTT CAAGGAGGAG GTAGCTAAAG AGTTTGGTAT ACCAATTCAA 
TTTCAGCGTT TTTGGCTGTG GGCAAAGCGC CAAAACCACA CATATCGTCC TAATCGGCCA 
TTGACACATC TAGAAGAACA ACAATCTGTT GGACAGTTAA GGGAGATATC AAATAAGGTT 
CAAAATGCAG AACTAAAGTT GTTCTTGGAA GTAAATCTTG GGCCGGATTT ACACCCAAAC 
CCTCCACCTG AGAAGACAAA GGATGATATT TTGCTTTTCT TCAAGCTTTA TGATCCTGAG 
AAGGAAGAGC TAAATTATGT TGGAAGGCTT TTTGTGAAGA GTACTGGTAA GCCTGTAGAA 
ATTTTGTCAA AACTGAATGA AATGGTTGGT TATGCTCCTG ATGAAGAGAT TGAACTCTAT 
GAGGAAATCA AGTTTGATCC TAGTGTCATG TGTGAACCCA TTGATAAGAA ATTCACATTC 
CGAGCTAGCC AGCTTGAAGA TGGTGATATC ATTTGCTTTC AAAAAACACC TCCAATTGAA 
AGTGGTGAAT CATTCCGCTA TCCAGATGTT CCTTCATTTT TAGAATACGT GCACAATCGC 
CAAGTTGTTC ATTTTCGGTC CCTAGAAAAA CCCAAGGAAG ATGATTTTTG TCTTGAGATG 
TCAAAGCTTT TTACTTATGA TGACGTGGTA GAAAGAGTAG CTCGTCAGCT TGGTTTGGAT 
GATCCATCCA AAATCAGGCT CACATCTCAC AACTGTTACT CTCAGCAACC AAAACCCCAG 
CCAATTAAGT ACCGTGGGGT GGACCATTTG TCTGACATGC TGGTTCACTA CAATCTGATT 
TCGGATGTAT TGTACTATGA AGTATTGGAC ATCCCTCTTC CAGAATTACA AGGTTTAAAA 
ACTTTGAAAG TTGCTTTTCA TCATGCTGAA AAGGAGGAAG TTGTTAGTCA TAGTATTAGA 
CTGCCAAAAC AGAGTACAGT AGGGGATGTA ATTAATGCTC TTAAAACAAA GGTTGAGCTG 
TCTCATCCTA ATGCTGAAGT TAGGTTGCTT GAAGTTTTCT ATCACAAGAT CTATAAGGTT 
TTTCCTTCAA ATGAAAAAAT TGAGAACATT AATGATCAAT ACTGGACACT GCGTGCTGAA 
GAGATTCCTG AAGAAGAGAA AAATCTTGGA CCACAGGATC GCTTGATTCA TGTATACCAC 
TTTACAAAAG ACACAGCTCA GAACCAAATG CAAATTCAGA ATTTCGGGGA GCCTTTTTTC 
TTGGTCATCC ATGAGGGTGA AACTCTGGCT GAAGTTAAGG TGCGGATACA GAAGAAGCTG 
CTGGTTCCAG AGGAAGAATT TGCTAAGTGG AGGTTTGCCT TTCTTTCACT AGGTCGCCCA 
GAGTACCTTC AAGACTCTGA CATTGTCTCC AGTCGTTTTC AGAGAAGAGA TGTTTATGGT 
GCTTGGGAGC AGTATCTTGG ACTAGAGCAT TCTGACACTG CTCCTAAAAG GGCTTATGCA 
GCTAATCAGA ACCGTCACAC ATTTGAGAAG CCGGTAAAAA TCTACAACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100266796 ( XM_010653329.2 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_010651631.1 Vitis vinifera ubiquitin carboxyl-terminal hydrolase 12 (LOC100266796), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OVa33145
Clone ID Related Accession (Same CDS sequence)	XM_010653329.2
Accession Version	XM_010653329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479916800000
Organism	Vitis vinifera(wine grape)
Product	ubiquitin carboxyl-terminal hydrolase 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012012.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 25, 2016 this sequence version replaced XM_010653329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTTC CCCACACAGA TTTGGCCGAC GGCCATCAAC CTATGGAAGT TGTTGCACAA 
GAGGAAACTA CTAGTACTGT GGAAAACCAG CCAGTTGAGG ATCCTCCTAC ATCAAGGTTC 
ACATGGAGAA TTGAGAACTT TTCTAGGTTG AATACAAAGA AGCACTATTC CGAAAATTTC 
ATTGTGGGCG GCTATAAGTG GCGGGTGCTT ATTTTTCCAA AGGGAAACAA TGTTGAACAT 
TTGTCTATGT ACTTGGATGT TGCGGATTCA TCAAGTTTGC CCTATGGGTG GAGTAGATAT 
GCACAATTCA GCTTGGCTGT GGTAAATCAA ATTCATAACA AATATACAGT GAGAAAAGAC 
ACTCAACACC AGTTTAATGC ACGAGAAAGT GATTGGGGTT TCACTTCTTT TATGCCTCTT 
AGTGAACTAT ACGATCCAGG TAGGGGTTTT CTTGTGAGTG ATACATGTAT AGTTGAAGCA 
GAGGTTGCTG TCCGCAGAGT TGTGGATTAC TGGACATATG ACTCAAAAAA GGAAACTGGT 
TATGTTGGAC TTAAGAACCA AGGAGCAACT TGTTACATGA ACTCTCTCCT CCAAACTCTC 
TATCATATTC CCTACTTTAG AAAGGCTGTG TACCATATGC CAACGACTGA GAATGATATG 
CCATCTGGAA GCATTCCTTT GGCTTTGCAG AGTTTATTCT ATAAGCTTCA ATACAGTGAC 
AGCAGCGTTG CAACGAAAGA GTTGACAAAA TCGTTTGGTT GGGACACATA TGATTCTTTC 
TTGCAGCATG ATGTGCAAGA ACTCAATAGG GTCCTCTGTG AAAAGCTTGA AGACAAAATG 
AAGGGAACTG TTGTGGAGGG CACTATACAG CAGTTATTTG AAGGGCACCA TATGAACTAC 
ATTGAATGCA TCAATGTGGA CTACAAATCT ACAAGGAAGG AGTCATTTTA TGACCTTCAG 
CTGGATGTCA AAGGTTGTCA TGATGTTTAT GCTTCTTTTG ATAAGTACGT AGAAGTTGAG 
CGCCTAGAAG GGGATAACAA ATATCATGCT GAGCACCATG GATTGCAGGA TGCTAGGAAG 
GGTGTCTTAT TCATTGACTT CCCTCCTGTC CTTCAACTTC AGCTGAAGCG ATTTGAATAT 
GATTTTATGC GGGACACTAT GGTAAAGATA AATGATCGCT ATGAGTTCCC TCTGCAACTT 
GATCTTGATC GAGAGAATGG CAAATATTTA TCTCCTGATG CCAATAGGAC TGTTCGCAAC 
CTTTACGCCC TTCACAGTGT TCTAGTTCAC AGTGGCGGAG TGCATGGTGG ACATTATTAT 
GCCTTTATAA GACCCACCCT TTCTGATCAG TGGTATAAAT TTGATGATGA AAGGGTGACA 
AAAGAAGACG TGAAGAGGGC TTTGGAGGAG CAATATGGTG GTGAAGAAGA GCTGCCCCAG 
ACAAATCCTG GATTGAATAA CACCCCCTTT AAATTTACGA AGTATTCAAA TGCATACATG 
CTGGTGTATA TACGTGAAAG TGACAAGGAC AAAATAATTT GCAATGTGGA TGAGAAGGAC 
ATTGCTGAAC ATCTCCGGGA GAGGTTGAAG AAAGAACAAG AAGAAAAAGA GCACAAGAAA 
AAGGAAAAAG CCGAGTCACA TCTTTATACC ATTATTAAGG TTGCACGAGA TGATGACCTT 
GTAGAACATA TTGGAAGGGA TATATATTTT GATCTTGTGG ATCATGACAA AGTTAGAAGT 
TTTCGTATCC AGAAGCAGAT GCCTTTCAAC TTTTTCAAGG AGGAGGTAGC TAAAGAGTTT 
GGTATACCAA TTCAATTTCA GCGTTTTTGG CTGTGGGCAA AGCGCCAAAA CCACACATAT 
CGTCCTAATC GGCCATTGAC ACATCTAGAA GAACAACAAT CTGTTGGACA GTTAAGGGAG 
ATATCAAATA AGGTTCAAAA TGCAGAACTA AAGTTGTTCT TGGAAGTAAA TCTTGGGCCG 
GATTTACACC CAAACCCTCC ACCTGAGAAG ACAAAGGATG ATATTTTGCT TTTCTTCAAG 
CTTTATGATC CTGAGAAGGA AGAGCTAAAT TATGTTGGAA GGCTTTTTGT GAAGAGTACT 
GGTAAGCCTG TAGAAATTTT GTCAAAACTG AATGAAATGG TTGGTTATGC TCCTGATGAA 
GAGATTGAAC TCTATGAGGA AATCAAGTTT GATCCTAGTG TCATGTGTGA ACCCATTGAT 
AAGAAATTCA CATTCCGAGC TAGCCAGCTT GAAGATGGTG ATATCATTTG CTTTCAAAAA 
ACACCTCCAA TTGAAAGTGG TGAATCATTC CGCTATCCAG ATGTTCCTTC ATTTTTAGAA 
TACGTGCACA ATCGCCAAGT TGTTCATTTT CGGTCCCTAG AAAAACCCAA GGAAGATGAT 
TTTTGTCTTG AGATGTCAAA GCTTTTTACT TATGATGACG TGGTAGAAAG AGTAGCTCGT 
CAGCTTGGTT TGGATGATCC ATCCAAAATC AGGCTCACAT CTCACAACTG TTACTCTCAG 
CAACCAAAAC CCCAGCCAAT TAAGTACCGT GGGGTGGACC ATTTGTCTGA CATGCTGGTT 
CACTACAATC TGATTTCGGA TGTATTGTAC TATGAAGTAT TGGACATCCC TCTTCCAGAA 
TTACAAGGTT TAAAAACTTT GAAAGTTGCT TTTCATCATG CTGAAAAGGA GGAAGTTGTT 
AGTCATAGTA TTAGACTGCC AAAACAGAGT ACAGTAGGGG ATGTAATTAA TGCTCTTAAA 
ACAAAGGTTG AGCTGTCTCA TCCTAATGCT GAAGTTAGGT TGCTTGAAGT TTTCTATCAC 
AAGATCTATA AGGTTTTTCC TTCAAATGAA AAAATTGAGA ACATTAATGA TCAATACTGG 
ACACTGCGTG CTGAAGAGAT TCCTGAAGAA GAGAAAAATC TTGGACCACA GGATCGCTTG 
ATTCATGTAT ACCACTTTAC AAAAGACACA GCTCAGAACC AAATGCAAAT TCAGAATTTC 
GGGGAGCCTT TTTTCTTGGT CATCCATGAG GGTGAAACTC TGGCTGAAGT TAAGGTGCGG 
ATACAGAAGA AGCTGCTGGT TCCAGAGGAA GAATTTGCTA AGTGGAGGTT TGCCTTTCTT 
TCACTAGGTC GCCCAGAGTA CCTTCAAGAC TCTGACATTG TCTCCAGTCG TTTTCAGAGA 
AGAGATGTTT ATGGTGCTTG GGAGCAGTAT CTTGGACTAG AGCATTCTGA CACTGCTCCT 
AAAAGGGCTT ATGCAGCTAA TCAGAACCGT CACACATTTG AGAAGCCGGT AAAAATCTAC 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010651631.1
CDS	62..3367
Translation	MLVPHTDLADGHQPMEVVAQEETTSTVENQPVEDPPTSRFTWRIENFSRLNTKKHYSENFIVGGYKWRVLIFPKGNNVEHLSMYLDVADSSSLPYGWSRYAQFSLAVVNQIHNKYTVRKDTQHQFNARESDWGFTSFMPLSELYDPGRGFLVSDTCIVEAEVAVRRVVDYWTYDSKKETGYVGLKNQGATCYMNSLLQTLYHIPYFRKAVYHMPTTENDMPSGSIPLALQSLFYKLQYSDSSVATKELTKSFGWDTYDSFLQHDVQELNRVLCEKLEDKMKGTVVEGTIQQLFEGHHMNYIECINVDYKSTRKESFYDLQLDVKGCHDVYASFDKYVEVERLEGDNKYHAEHHGLQDARKGVLFIDFPPVLQLQLKRFEYDFMRDTMVKINDRYEFPLQLDLDRENGKYLSPDANRTVRNLYALHSVLVHSGGVHGGHYYAFIRPTLSDQWYKFDDERVTKEDVKRALEEQYGGEEELPQTNPGLNNTPFKFTKYSNAYMLVYIRESDKDKIICNVDEKDIAEHLRERLKKEQEEKEHKKKEKAESHLYTIIKVARDDDLVEHIGRDIYFDLVDHDKVRSFRIQKQMPFNFFKEEVAKEFGIPIQFQRFWLWAKRQNHTYRPNRPLTHLEEQQSVGQLREISNKVQNAELKLFLEVNLGPDLHPNPPPEKTKDDILLFFKLYDPEKEELNYVGRLFVKSTGKPVEILSKLNEMVGYAPDEEIELYEEIKFDPSVMCEPIDKKFTFRASQLEDGDIICFQKTPPIESGESFRYPDVPSFLEYVHNRQVVHFRSLEKPKEDDFCLEMSKLFTYDDVVERVARQLGLDDPSKIRLTSHNCYSQQPKPQPIKYRGVDHLSDMLVHYNLISDVLYYEVLDIPLPELQGLKTLKVAFHHAEKEEVVSHSIRLPKQSTVGDVINALKTKVELSHPNAEVRLLEVFYHKIYKVFPSNEKIENINDQYWTLRAEEIPEEEKNLGPQDRLIHVYHFTKDTAQNQMQIQNFGEPFFLVIHEGETLAEVKVRIQKKLLVPEEEFAKWRFAFLSLGRPEYLQDSDIVSSRFQRRDVYGAWEQYLGLEHSDTAPKRAYAANQNRHTFEKPVKIYN

Target ORF information:

RefSeq Version	XM_010653329.2
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera ubiquitin carboxyl-terminal hydrolase 12 (LOC100266796), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010653329.2

ORF Insert Sequence:

ATGCTGGTTC CCCACACAGA TTTGGCCGAC GGCCATCAAC CTATGGAAGT TGTTGCACAA 
GAGGAAACTA CTAGTACTGT GGAAAACCAG CCAGTTGAGG ATCCTCCTAC ATCAAGGTTC 
ACATGGAGAA TTGAGAACTT TTCTAGGTTG AATACAAAGA AGCACTATTC CGAAAATTTC 
ATTGTGGGCG GCTATAAGTG GCGGGTGCTT ATTTTTCCAA AGGGAAACAA TGTTGAACAT 
TTGTCTATGT ACTTGGATGT TGCGGATTCA TCAAGTTTGC CCTATGGGTG GAGTAGATAT 
GCACAATTCA GCTTGGCTGT GGTAAATCAA ATTCATAACA AATATACAGT GAGAAAAGAC 
ACTCAACACC AGTTTAATGC ACGAGAAAGT GATTGGGGTT TCACTTCTTT TATGCCTCTT 
AGTGAACTAT ACGATCCAGG TAGGGGTTTT CTTGTGAGTG ATACATGTAT AGTTGAAGCA 
GAGGTTGCTG TCCGCAGAGT TGTGGATTAC TGGACATATG ACTCAAAAAA GGAAACTGGT 
TATGTTGGAC TTAAGAACCA AGGAGCAACT TGTTACATGA ACTCTCTCCT CCAAACTCTC 
TATCATATTC CCTACTTTAG AAAGGCTGTG TACCATATGC CAACGACTGA GAATGATATG 
CCATCTGGAA GCATTCCTTT GGCTTTGCAG AGTTTATTCT ATAAGCTTCA ATACAGTGAC 
AGCAGCGTTG CAACGAAAGA GTTGACAAAA TCGTTTGGTT GGGACACATA TGATTCTTTC 
TTGCAGCATG ATGTGCAAGA ACTCAATAGG GTCCTCTGTG AAAAGCTTGA AGACAAAATG 
AAGGGAACTG TTGTGGAGGG CACTATACAG CAGTTATTTG AAGGGCACCA TATGAACTAC 
ATTGAATGCA TCAATGTGGA CTACAAATCT ACAAGGAAGG AGTCATTTTA TGACCTTCAG 
CTGGATGTCA AAGGTTGTCA TGATGTTTAT GCTTCTTTTG ATAAGTACGT AGAAGTTGAG 
CGCCTAGAAG GGGATAACAA ATATCATGCT GAGCACCATG GATTGCAGGA TGCTAGGAAG 
GGTGTCTTAT TCATTGACTT CCCTCCTGTC CTTCAACTTC AGCTGAAGCG ATTTGAATAT 
GATTTTATGC GGGACACTAT GGTAAAGATA AATGATCGCT ATGAGTTCCC TCTGCAACTT 
GATCTTGATC GAGAGAATGG CAAATATTTA TCTCCTGATG CCAATAGGAC TGTTCGCAAC 
CTTTACGCCC TTCACAGTGT TCTAGTTCAC AGTGGCGGAG TGCATGGTGG ACATTATTAT 
GCCTTTATAA GACCCACCCT TTCTGATCAG TGGTATAAAT TTGATGATGA AAGGGTGACA 
AAAGAAGACG TGAAGAGGGC TTTGGAGGAG CAATATGGTG GTGAAGAAGA GCTGCCCCAG 
ACAAATCCTG GATTGAATAA CACCCCCTTT AAATTTACGA AGTATTCAAA TGCATACATG 
CTGGTGTATA TACGTGAAAG TGACAAGGAC AAAATAATTT GCAATGTGGA TGAGAAGGAC 
ATTGCTGAAC ATCTCCGGGA GAGGTTGAAG AAAGAACAAG AAGAAAAAGA GCACAAGAAA 
AAGGAAAAAG CCGAGTCACA TCTTTATACC ATTATTAAGG TTGCACGAGA TGATGACCTT 
GTAGAACATA TTGGAAGGGA TATATATTTT GATCTTGTGG ATCATGACAA AGTTAGAAGT 
TTTCGTATCC AGAAGCAGAT GCCTTTCAAC TTTTTCAAGG AGGAGGTAGC TAAAGAGTTT 
GGTATACCAA TTCAATTTCA GCGTTTTTGG CTGTGGGCAA AGCGCCAAAA CCACACATAT 
CGTCCTAATC GGCCATTGAC ACATCTAGAA GAACAACAAT CTGTTGGACA GTTAAGGGAG 
ATATCAAATA AGGTTCAAAA TGCAGAACTA AAGTTGTTCT TGGAAGTAAA TCTTGGGCCG 
GATTTACACC CAAACCCTCC ACCTGAGAAG ACAAAGGATG ATATTTTGCT TTTCTTCAAG 
CTTTATGATC CTGAGAAGGA AGAGCTAAAT TATGTTGGAA GGCTTTTTGT GAAGAGTACT 
GGTAAGCCTG TAGAAATTTT GTCAAAACTG AATGAAATGG TTGGTTATGC TCCTGATGAA 
GAGATTGAAC TCTATGAGGA AATCAAGTTT GATCCTAGTG TCATGTGTGA ACCCATTGAT 
AAGAAATTCA CATTCCGAGC TAGCCAGCTT GAAGATGGTG ATATCATTTG CTTTCAAAAA 
ACACCTCCAA TTGAAAGTGG TGAATCATTC CGCTATCCAG ATGTTCCTTC ATTTTTAGAA 
TACGTGCACA ATCGCCAAGT TGTTCATTTT CGGTCCCTAG AAAAACCCAA GGAAGATGAT 
TTTTGTCTTG AGATGTCAAA GCTTTTTACT TATGATGACG TGGTAGAAAG AGTAGCTCGT 
CAGCTTGGTT TGGATGATCC ATCCAAAATC AGGCTCACAT CTCACAACTG TTACTCTCAG 
CAACCAAAAC CCCAGCCAAT TAAGTACCGT GGGGTGGACC ATTTGTCTGA CATGCTGGTT 
CACTACAATC TGATTTCGGA TGTATTGTAC TATGAAGTAT TGGACATCCC TCTTCCAGAA 
TTACAAGGTT TAAAAACTTT GAAAGTTGCT TTTCATCATG CTGAAAAGGA GGAAGTTGTT 
AGTCATAGTA TTAGACTGCC AAAACAGAGT ACAGTAGGGG ATGTAATTAA TGCTCTTAAA 
ACAAAGGTTG AGCTGTCTCA TCCTAATGCT GAAGTTAGGT TGCTTGAAGT TTTCTATCAC 
AAGATCTATA AGGTTTTTCC TTCAAATGAA AAAATTGAGA ACATTAATGA TCAATACTGG 
ACACTGCGTG CTGAAGAGAT TCCTGAAGAA GAGAAAAATC TTGGACCACA GGATCGCTTG 
ATTCATGTAT ACCACTTTAC AAAAGACACA GCTCAGAACC AAATGCAAAT TCAGAATTTC 
GGGGAGCCTT TTTTCTTGGT CATCCATGAG GGTGAAACTC TGGCTGAAGT TAAGGTGCGG 
ATACAGAAGA AGCTGCTGGT TCCAGAGGAA GAATTTGCTA AGTGGAGGTT TGCCTTTCTT 
TCACTAGGTC GCCCAGAGTA CCTTCAAGAC TCTGACATTG TCTCCAGTCG TTTTCAGAGA 
AGAGATGTTT ATGGTGCTTG GGAGCAGTAT CTTGGACTAG AGCATTCTGA CACTGCTCCT 
AAAAGGGCTT ATGCAGCTAA TCAGAACCGT CACACATTTG AGAAGCCGGT AAAAATCTAC 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100266796 cDNA ORF clone, Vitis vinifera(wine grape)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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