ORF » Species Summary » Vitis vinifera » LOC100249854 cDNA ORF clone

LOC100249854 cDNA ORF clone, Vitis vinifera(wine grape)

The following LOC100249854 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100249854 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OVa21423 XM_002277043.3
Latest version!		 Vitis vinifera leucine--tRNA ligase, cytoplasmic (LOC100249854), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OVa39349 XM_019217877.1
Latest version!		 Vitis vinifera leucine--tRNA ligase, cytoplasmic (LOC100249854), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC100249854 ( XM_002277043.3 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_002277079.1 Vitis vinifera leucine--tRNA ligase, cytoplasmic (LOC100249854), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OVa21423
    Clone ID Related Accession (Same CDS sequence) XM_002277043.3
    Accession Version XM_002277043.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1479657600000
    Organism Vitis vinifera(wine grape)
    Product leucine--tRNA ligase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003724191.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 22, 2016 this sequence version replaced XM_002277043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTTCAA ACACTGAAGG TGGGAAGAGC TTTGCCAGGA GAGACCGGCT GCTGGAAATA 
    GAGGCTCAGG TCCAAAAATG GTGGGGAGAA AAAGAGATTT TCAGGGCTGA TTCTCTCAAA 
    ACGCCTCCTA AACCGGGTGA AAGGTTCTTT GGGAATTTCC CATATCCTTA CATGAATGGC 
    TACCTGCACC TTGGTCATGC ATTTTCCCTT TCAAAATTGG AGTTTGCTTC AGCATATCAC 
    CGACTGAGAG GCGCCAATGT CCTCTTGCCT TTCGCTTTCC ACTGTACTGG GATGCCAGTC 
    AAGGCCTCTG CTGATAAACT ATCCCGGGAA ATCCAATTGT TTGGTGATCC ACCCATCTTC 
    CCTTCCACCC CAGAGGACCA AATCAGTGAC CCAGACCAGG AACTGGGGGA AGAGGTTGGG 
    GAAAACCAGG GTGTGGCAGA AAAGTCTAAA GGCAAGAAGT CCAAAGCAGC AGCAAAGGCG 
    AGCAGCACCA AGTTTCAGTG GGAGATTATG CGGAGTTATG GCCTCTCTGA TGCTGAGATT 
    TCTAAGTTCC AGGATCCATA TCACTGGCTA ATCTATTTCC CCCCTCTGGC CATGGAAGAC 
    CTCAAGGCTT TTGGCTTGGG CTGCGACTGG AGACGGACCT TCATCACCAC AGAAGTGAAC 
    CCCTTCTATG ACTCATTTGT CAGGTGGCAG ATGAGGAAGC TGAAGAAGAT GGGGAAGATT 
    GTTAAGGACT TGAGATACAC TATCTACTCT CCTTTAGACG GCCAGCCGTG TGCTGATCAT 
    GACCGAGCTT CTGGTGAAGG CGTTTTGCCT CAAGAGTACA CTCTTGTAAA AATGGAGGTG 
    ATCCCACAAT TTCCCCCAAA GTTGAGAGCT CTGGAAGGGA GGAAGGTGTT TCTTGCTGCT 
    GCAACATTGA GGCCTGAGAC GATGTATGGA CAAACAAACG CGTGGGTGTT GCCAGATGGG 
    AAATATGGAG CCTTTGAGAT CAATGATACT GATGTGTTCA TTTTATCTGA GAGGGCAGCT 
    CTTAATCTTG CATACCAGAA ACTGTCCCGT GTTCCAGAAA AGCCCAGTTG TTTGGCTGAG 
    CTGACCGGCT ATGATTTGAT CGGTTTGCCC CTCAAGTCCC CATTAGCATT CAATGAGATC 
    ATATATGCTC TCCCCATGCT TTCGATTCTC ATTGACAAAG GTACTGGAAT TGTGACAAGC 
    GTGCCAAGTG ATTCACCCGA CGATTTCATG GCTCTCCATG ATTTGAAAAC AAAGCCAGTT 
    TTCAGAGCTA AGTTTGGTGT AAAGGACGAG TGGGTCTTGC CATTTGAGGT CATTCCAATT 
    ATCAATACCC CAGAATTTGG AGATAAGTCA GCTGAGAAAA TCTGCAAAGA TTATGGTATT 
    CAGAGCCAGA ATGAGAAAGA GAAGCTTGCT GAGGCCAAGA AACTGATTTA CAGGGGAGGA 
    TTCTATGAGG GAACTTTGAT TGTTGGAGAA TATGCAGGAA TGAGAGTCCA GGAAGCTAAG 
    AATCTGATCA GGAGCAAGCT TCTGGAGCTC GGTCAAGCTG TTGTATATAG CGAGCCAGAG 
    AAGAAGGTCA TCTCAAGATC AGGGGATGAG TGTGTGGTGG CTCTGACTGA TCAGTGGTAT 
    ATCACCTATG GGGAGCCTGA ATGGAAGCAG AAGGCAGAGG AGTGCTTGGC CAGCATGAAT 
    CTGTACTCCA ATGAGGTGCG GCATGGTTTT GAGCACACAT TGAGCTGGCT GAATCAGTGG 
    GCTTGTTCCC GTTCCTTTGG GCTTGGGACT CGTCTTCCAT GGGATGAAGA CTTCCTCGTC 
    GAGTCCTTAT CTGATTCGAC TCTATACATG GCATACTATA CCATATCTCA TCTGTTACAG 
    AAAGGGAACT TGTATGGTTC TGATACTTCT TCGGTTAAAC CAGAGCAAAT GACAGATGAG 
    GTTTGGGACT TTGTGTTTTG TGGCAGCCCA TTCCCCAAAT CATCTGATAT TTGCCCCAGT 
    GTTCTGAGGA AGATGAAGCA GGAGTTTGAA TATTGGTATC CATTTGATGT CAGGACTTCT 
    GGCAAAGACC TGATTCAGAA TCATCTCACT TTTTGTATAT ACAACCACAC AGCAATCCTA 
    GCCAAGCATC ACTGGCCTAG GGGATTCAGA TGCAATGGGC ACATTATGCT GAATTCTGAG 
    AAAATGTCCA AGTCCACTGG AAACTTCATG ACAATACGCC AAGCTATCCA GGAATTCTCT 
    GCTGACGCCA CACGATTCTC CCTTGCTGAT GCTGGAGATG GAATGGATGA TGCCAACTTT 
    GTCTCTGAGA CGGCAAATGC TGCAATCTTA CGCCTCACAA AAGAGATATC ATGGATGCAG 
    GAGGTTATAG AGGGGGAGTC ATCTTTCAGA AAGGGGCCCT CTTCTACTTA TGCTGATCGC 
    GTGTTTGCCA ATGAGATCAA CATTGCAGTC AAAATGACTG AGAAGAACTA CAGTGAGTTC 
    ATGTTCAGGG AAGCTCTCAA GACTGGGTTT TATGATCTCC AAGCTGCCAG GGATGAGTAT 
    AGATTTTCTT GTGGTATGGG GGGCATGAAT CGTGATCTGT TATGGCGGTT TATGGATGTT 
    CAAACTCGTC TTATGACTCC AATCTGCCCT CATTTTGCTG AATATGTTTG GAAGGAGCTT 
    CTGAGGAAAG AAGGGTTTGT TGTGAAAGCA GGATGGCCTG AAGCTGATAC ACTTGACCTC 
    ACTCTCAAGC TTGCCAACAA GTATCTGCAG GACTCAATTG TCTCAATGAG GAAGCTGCTC 
    CAAAAACAAG TTTCAGGCCC AAAAAGGGCC GACAAAAGCA TCTCCTCTAG TGCAGAAAAC 
    AGACCAACTG TCGGACTAAT ATACATGGCC GAGCAATACG ACGGATGGAA AGCAGAATGC 
    TTGAAAATAC TACAAAGCAA GTTCAACACA GAAACGAGCA GTTTTGCACC AGACCAAGAG 
    ATCTTGGAGG CATTACAGCA GAGTGAAATT GGGCAGGAAG GAAACTTTAA GGAGACCCAG 
    AAGCTGTGTA TGCCTTTCCT GAGGTTCAAG AAGGATGAGG CCATTGCTGT TGGGCATCAG 
    GCCTTGGATC TGAAGCTACC CTTCGGAGAA GTGGAGGTCC TGGGGGAGAA CTTGGAGTTG 
    ATCAAGAGGC AGCTTGGTCT AGAGGAGGTT GAAGTTCTGT CTGCTGCAGA CCCTGAGGCT 
    ATTCAGAAAG CTGGGCAGTA TGCCTCACTG CTAAACCAGA ATCCGCCTTC TCCAGGAAAC 
    CCAACTGCCA TCTTCTTGAG TGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002277079.1
    CDS130..3396
    Translation

    Target ORF information:

    RefSeq Version XM_002277043.3
    Organism Vitis vinifera(wine grape)
    Definition Vitis vinifera leucine--tRNA ligase, cytoplasmic (LOC100249854), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_002277043.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGTTTCAA ACACTGAAGG TGGGAAGAGC TTTGCCAGGA GAGACCGGCT GCTGGAAATA 
    GAGGCTCAGG TCCAAAAATG GTGGGGAGAA AAAGAGATTT TCAGGGCTGA TTCTCTCAAA 
    ACGCCTCCTA AACCGGGTGA AAGGTTCTTT GGGAATTTCC CATATCCTTA CATGAATGGC 
    TACCTGCACC TTGGTCATGC ATTTTCCCTT TCAAAATTGG AGTTTGCTTC AGCATATCAC 
    CGACTGAGAG GCGCCAATGT CCTCTTGCCT TTCGCTTTCC ACTGTACTGG GATGCCAGTC 
    AAGGCCTCTG CTGATAAACT ATCCCGGGAA ATCCAATTGT TTGGTGATCC ACCCATCTTC 
    CCTTCCACCC CAGAGGACCA AATCAGTGAC CCAGACCAGG AACTGGGGGA AGAGGTTGGG 
    GAAAACCAGG GTGTGGCAGA AAAGTCTAAA GGCAAGAAGT CCAAAGCAGC AGCAAAGGCG 
    AGCAGCACCA AGTTTCAGTG GGAGATTATG CGGAGTTATG GCCTCTCTGA TGCTGAGATT 
    TCTAAGTTCC AGGATCCATA TCACTGGCTA ATCTATTTCC CCCCTCTGGC CATGGAAGAC 
    CTCAAGGCTT TTGGCTTGGG CTGCGACTGG AGACGGACCT TCATCACCAC AGAAGTGAAC 
    CCCTTCTATG ACTCATTTGT CAGGTGGCAG ATGAGGAAGC TGAAGAAGAT GGGGAAGATT 
    GTTAAGGACT TGAGATACAC TATCTACTCT CCTTTAGACG GCCAGCCGTG TGCTGATCAT 
    GACCGAGCTT CTGGTGAAGG CGTTTTGCCT CAAGAGTACA CTCTTGTAAA AATGGAGGTG 
    ATCCCACAAT TTCCCCCAAA GTTGAGAGCT CTGGAAGGGA GGAAGGTGTT TCTTGCTGCT 
    GCAACATTGA GGCCTGAGAC GATGTATGGA CAAACAAACG CGTGGGTGTT GCCAGATGGG 
    AAATATGGAG CCTTTGAGAT CAATGATACT GATGTGTTCA TTTTATCTGA GAGGGCAGCT 
    CTTAATCTTG CATACCAGAA ACTGTCCCGT GTTCCAGAAA AGCCCAGTTG TTTGGCTGAG 
    CTGACCGGCT ATGATTTGAT CGGTTTGCCC CTCAAGTCCC CATTAGCATT CAATGAGATC 
    ATATATGCTC TCCCCATGCT TTCGATTCTC ATTGACAAAG GTACTGGAAT TGTGACAAGC 
    GTGCCAAGTG ATTCACCCGA CGATTTCATG GCTCTCCATG ATTTGAAAAC AAAGCCAGTT 
    TTCAGAGCTA AGTTTGGTGT AAAGGACGAG TGGGTCTTGC CATTTGAGGT CATTCCAATT 
    ATCAATACCC CAGAATTTGG AGATAAGTCA GCTGAGAAAA TCTGCAAAGA TTATGGTATT 
    CAGAGCCAGA ATGAGAAAGA GAAGCTTGCT GAGGCCAAGA AACTGATTTA CAGGGGAGGA 
    TTCTATGAGG GAACTTTGAT TGTTGGAGAA TATGCAGGAA TGAGAGTCCA GGAAGCTAAG 
    AATCTGATCA GGAGCAAGCT TCTGGAGCTC GGTCAAGCTG TTGTATATAG CGAGCCAGAG 
    AAGAAGGTCA TCTCAAGATC AGGGGATGAG TGTGTGGTGG CTCTGACTGA TCAGTGGTAT 
    ATCACCTATG GGGAGCCTGA ATGGAAGCAG AAGGCAGAGG AGTGCTTGGC CAGCATGAAT 
    CTGTACTCCA ATGAGGTGCG GCATGGTTTT GAGCACACAT TGAGCTGGCT GAATCAGTGG 
    GCTTGTTCCC GTTCCTTTGG GCTTGGGACT CGTCTTCCAT GGGATGAAGA CTTCCTCGTC 
    GAGTCCTTAT CTGATTCGAC TCTATACATG GCATACTATA CCATATCTCA TCTGTTACAG 
    AAAGGGAACT TGTATGGTTC TGATACTTCT TCGGTTAAAC CAGAGCAAAT GACAGATGAG 
    GTTTGGGACT TTGTGTTTTG TGGCAGCCCA TTCCCCAAAT CATCTGATAT TTGCCCCAGT 
    GTTCTGAGGA AGATGAAGCA GGAGTTTGAA TATTGGTATC CATTTGATGT CAGGACTTCT 
    GGCAAAGACC TGATTCAGAA TCATCTCACT TTTTGTATAT ACAACCACAC AGCAATCCTA 
    GCCAAGCATC ACTGGCCTAG GGGATTCAGA TGCAATGGGC ACATTATGCT GAATTCTGAG 
    AAAATGTCCA AGTCCACTGG AAACTTCATG ACAATACGCC AAGCTATCCA GGAATTCTCT 
    GCTGACGCCA CACGATTCTC CCTTGCTGAT GCTGGAGATG GAATGGATGA TGCCAACTTT 
    GTCTCTGAGA CGGCAAATGC TGCAATCTTA CGCCTCACAA AAGAGATATC ATGGATGCAG 
    GAGGTTATAG AGGGGGAGTC ATCTTTCAGA AAGGGGCCCT CTTCTACTTA TGCTGATCGC 
    GTGTTTGCCA ATGAGATCAA CATTGCAGTC AAAATGACTG AGAAGAACTA CAGTGAGTTC 
    ATGTTCAGGG AAGCTCTCAA GACTGGGTTT TATGATCTCC AAGCTGCCAG GGATGAGTAT 
    AGATTTTCTT GTGGTATGGG GGGCATGAAT CGTGATCTGT TATGGCGGTT TATGGATGTT 
    CAAACTCGTC TTATGACTCC AATCTGCCCT CATTTTGCTG AATATGTTTG GAAGGAGCTT 
    CTGAGGAAAG AAGGGTTTGT TGTGAAAGCA GGATGGCCTG AAGCTGATAC ACTTGACCTC 
    ACTCTCAAGC TTGCCAACAA GTATCTGCAG GACTCAATTG TCTCAATGAG GAAGCTGCTC 
    CAAAAACAAG TTTCAGGCCC AAAAAGGGCC GACAAAAGCA TCTCCTCTAG TGCAGAAAAC 
    AGACCAACTG TCGGACTAAT ATACATGGCC GAGCAATACG ACGGATGGAA AGCAGAATGC 
    TTGAAAATAC TACAAAGCAA GTTCAACACA GAAACGAGCA GTTTTGCACC AGACCAAGAG 
    ATCTTGGAGG CATTACAGCA GAGTGAAATT GGGCAGGAAG GAAACTTTAA GGAGACCCAG 
    AAGCTGTGTA TGCCTTTCCT GAGGTTCAAG AAGGATGAGG CCATTGCTGT TGGGCATCAG 
    GCCTTGGATC TGAAGCTACC CTTCGGAGAA GTGGAGGTCC TGGGGGAGAA CTTGGAGTTG 
    ATCAAGAGGC AGCTTGGTCT AGAGGAGGTT GAAGTTCTGT CTGCTGCAGA CCCTGAGGCT 
    ATTCAGAAAG CTGGGCAGTA TGCCTCACTG CTAAACCAGA ATCCGCCTTC TCCAGGAAAC 
    CCAACTGCCA TCTTCTTGAG TGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100249854 ( XM_019217877.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019073422.1 Vitis vinifera leucine--tRNA ligase, cytoplasmic (LOC100249854), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OVa39349
    Clone ID Related Accession (Same CDS sequence) XM_019217877.1
    Accession Version XM_019217877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1479657600000
    Organism Vitis vinifera(wine grape)
    Product leucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003724191.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGCGTT CATGCCCATT TCATAGTAAG GAGATGGTTT CAAACACTGA AGGTGGGAAG 
    AGCTTTGCCA GGAGAGACCG GCTGCTGGAA ATAGAGGCTC AGGTCCAAAA ATGGTGGGGA 
    GAAAAAGAGA TTTTCAGGGC TGATTCTCTC AAAACGCCTC CTAAACCGGG TGAAAGGTTC 
    TTTGGGAATT TCCCATATCC TTACATGAAT GGCTACCTGC ACCTTGGTCA TGCATTTTCC 
    CTTTCAAAAT TGGAGTTTGC TTCAGCATAT CACCGACTGA GAGGCGCCAA TGTCCTCTTG 
    CCTTTCGCTT TCCACTGTAC TGGGATGCCA GTCAAGGCCT CTGCTGATAA ACTATCCCGG 
    GAAATCCAAT TGTTTGGTGA TCCACCCATC TTCCCTTCCA CCCCAGAGGA CCAAATCAGT 
    GACCCAGACC AGGAACTGGG GGAAGAGGTT GGGGAAAACC AGGGTGTGGC AGAAAAGTCT 
    AAAGGCAAGA AGTCCAAAGC AGCAGCAAAG GCGAGCAGCA CCAAGTTTCA GTGGGAGATT 
    ATGCGGAGTT ATGGCCTCTC TGATGCTGAG ATTTCTAAGT TCCAGGATCC ATATCACTGG 
    CTAATCTATT TCCCCCCTCT GGCCATGGAA GACCTCAAGG CTTTTGGCTT GGGCTGCGAC 
    TGGAGACGGA CCTTCATCAC CACAGAAGTG AACCCCTTCT ATGACTCATT TGTCAGGTGG 
    CAGATGAGGA AGCTGAAGAA GATGGGGAAG ATTGTTAAGG ACTTGAGATA CACTATCTAC 
    TCTCCTTTAG ACGGCCAGCC GTGTGCTGAT CATGACCGAG CTTCTGGTGA AGGCGTTTTG 
    CCTCAAGAGT ACACTCTTGT AAAAATGGAG GTGATCCCAC AATTTCCCCC AAAGTTGAGA 
    GCTCTGGAAG GGAGGAAGGT GTTTCTTGCT GCTGCAACAT TGAGGCCTGA GACGATGTAT 
    GGACAAACAA ACGCGTGGGT GTTGCCAGAT GGGAAATATG GAGCCTTTGA GATCAATGAT 
    ACTGATGTGT TCATTTTATC TGAGAGGGCA GCTCTTAATC TTGCATACCA GAAACTGTCC 
    CGTGTTCCAG AAAAGCCCAG TTGTTTGGCT GAGCTGACCG GCTATGATTT GATCGGTTTG 
    CCCCTCAAGT CCCCATTAGC ATTCAATGAG ATCATATATG CTCTCCCCAT GCTTTCGATT 
    CTCATTGACA AAGGTACTGG AATTGTGACA AGCGTGCCAA GTGATTCACC CGACGATTTC 
    ATGGCTCTCC ATGATTTGAA AACAAAGCCA GTTTTCAGAG CTAAGTTTGG TGTAAAGGAC 
    GAGTGGGTCT TGCCATTTGA GGTCATTCCA ATTATCAATA CCCCAGAATT TGGAGATAAG 
    TCAGCTGAGA AAATCTGCAA AGATTATGGT ATTCAGAGCC AGAATGAGAA AGAGAAGCTT 
    GCTGAGGCCA AGAAACTGAT TTACAGGGGA GGATTCTATG AGGGAACTTT GATTGTTGGA 
    GAATATGCAG GAATGAGAGT CCAGGAAGCT AAGAATCTGA TCAGGAGCAA GCTTCTGGAG 
    CTCGGTCAAG CTGTTGTATA TAGCGAGCCA GAGAAGAAGG TCATCTCAAG ATCAGGGGAT 
    GAGTGTGTGG TGGCTCTGAC TGATCAGTGG TATATCACCT ATGGGGAGCC TGAATGGAAG 
    CAGAAGGCAG AGGAGTGCTT GGCCAGCATG AATCTGTACT CCAATGAGGT GCGGCATGGT 
    TTTGAGCACA CATTGAGCTG GCTGAATCAG TGGGCTTGTT CCCGTTCCTT TGGGCTTGGG 
    ACTCGTCTTC CATGGGATGA AGACTTCCTC GTCGAGTCCT TATCTGATTC GACTCTATAC 
    ATGGCATACT ATACCATATC TCATCTGTTA CAGAAAGGGA ACTTGTATGG TTCTGATACT 
    TCTTCGGTTA AACCAGAGCA AATGACAGAT GAGGTTTGGG ACTTTGTGTT TTGTGGCAGC 
    CCATTCCCCA AATCATCTGA TATTTGCCCC AGTGTTCTGA GGAAGATGAA GCAGGAGTTT 
    GAATATTGGT ATCCATTTGA TGTCAGGACT TCTGGCAAAG ACCTGATTCA GAATCATCTC 
    ACTTTTTGTA TATACAACCA CACAGCAATC CTAGCCAAGC ATCACTGGCC TAGGGGATTC 
    AGATGCAATG GGCACATTAT GCTGAATTCT GAGAAAATGT CCAAGTCCAC TGGAAACTTC 
    ATGACAATAC GCCAAGCTAT CCAGGAATTC TCTGCTGACG CCACACGATT CTCCCTTGCT 
    GATGCTGGAG ATGGAATGGA TGATGCCAAC TTTGTCTCTG AGACGGCAAA TGCTGCAATC 
    TTACGCCTCA CAAAAGAGAT ATCATGGATG CAGGAGGTTA TAGAGGGGGA GTCATCTTTC 
    AGAAAGGGGC CCTCTTCTAC TTATGCTGAT CGCGTGTTTG CCAATGAGAT CAACATTGCA 
    GTCAAAATGA CTGAGAAGAA CTACAGTGAG TTCATGTTCA GGGAAGCTCT CAAGACTGGG 
    TTTTATGATC TCCAAGCTGC CAGGGATGAG TATAGATTTT CTTGTGGTAT GGGGGGCATG 
    AATCGTGATC TGTTATGGCG GTTTATGGAT GTTCAAACTC GTCTTATGAC TCCAATCTGC 
    CCTCATTTTG CTGAATATGT TTGGAAGGAG CTTCTGAGGA AAGAAGGGTT TGTTGTGAAA 
    GCAGGATGGC CTGAAGCTGA TACACTTGAC CTCACTCTCA AGCTTGCCAA CAAGTATCTG 
    CAGGACTCAA TTGTCTCAAT GAGGAAGCTG CTCCAAAAAC AAGTTTCAGG CCCAAAAAGG 
    GCCGACAAAA GCATCTCCTC TAGTGCAGAA AACAGACCAA CTGTCGGACT AATATACATG 
    GCCGAGCAAT ACGACGGATG GAAAGCAGAA TGCTTGAAAA TACTACAAAG CAAGTTCAAC 
    ACAGAAACGA GCAGTTTTGC ACCAGACCAA GAGATCTTGG AGGCATTACA GCAGAGTGAA 
    ATTGGGCAGG AAGGAAACTT TAAGGAGACC CAGAAGCTGT GTATGCCTTT CCTGAGGTTC 
    AAGAAGGATG AGGCCATTGC TGTTGGGCAT CAGGCCTTGG ATCTGAAGCT ACCCTTCGGA 
    GAAGTGGAGG TCCTGGGGGA GAACTTGGAG TTGATCAAGA GGCAGCTTGG TCTAGAGGAG 
    GTTGAAGTTC TGTCTGCTGC AGACCCTGAG GCTATTCAGA AAGCTGGGCA GTATGCCTCA 
    CTGCTAAACC AGAATCCGCC TTCTCCAGGA AACCCAACTG CCATCTTCTT GAGTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_019073422.1
    CDS317..3616
    Translation

    Target ORF information:

    RefSeq Version XM_019217877.1
    Organism Vitis vinifera(wine grape)
    Definition Vitis vinifera leucine--tRNA ligase, cytoplasmic (LOC100249854), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_019217877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGATGCGTT CATGCCCATT TCATAGTAAG GAGATGGTTT CAAACACTGA AGGTGGGAAG 
    AGCTTTGCCA GGAGAGACCG GCTGCTGGAA ATAGAGGCTC AGGTCCAAAA ATGGTGGGGA 
    GAAAAAGAGA TTTTCAGGGC TGATTCTCTC AAAACGCCTC CTAAACCGGG TGAAAGGTTC 
    TTTGGGAATT TCCCATATCC TTACATGAAT GGCTACCTGC ACCTTGGTCA TGCATTTTCC 
    CTTTCAAAAT TGGAGTTTGC TTCAGCATAT CACCGACTGA GAGGCGCCAA TGTCCTCTTG 
    CCTTTCGCTT TCCACTGTAC TGGGATGCCA GTCAAGGCCT CTGCTGATAA ACTATCCCGG 
    GAAATCCAAT TGTTTGGTGA TCCACCCATC TTCCCTTCCA CCCCAGAGGA CCAAATCAGT 
    GACCCAGACC AGGAACTGGG GGAAGAGGTT GGGGAAAACC AGGGTGTGGC AGAAAAGTCT 
    AAAGGCAAGA AGTCCAAAGC AGCAGCAAAG GCGAGCAGCA CCAAGTTTCA GTGGGAGATT 
    ATGCGGAGTT ATGGCCTCTC TGATGCTGAG ATTTCTAAGT TCCAGGATCC ATATCACTGG 
    CTAATCTATT TCCCCCCTCT GGCCATGGAA GACCTCAAGG CTTTTGGCTT GGGCTGCGAC 
    TGGAGACGGA CCTTCATCAC CACAGAAGTG AACCCCTTCT ATGACTCATT TGTCAGGTGG 
    CAGATGAGGA AGCTGAAGAA GATGGGGAAG ATTGTTAAGG ACTTGAGATA CACTATCTAC 
    TCTCCTTTAG ACGGCCAGCC GTGTGCTGAT CATGACCGAG CTTCTGGTGA AGGCGTTTTG 
    CCTCAAGAGT ACACTCTTGT AAAAATGGAG GTGATCCCAC AATTTCCCCC AAAGTTGAGA 
    GCTCTGGAAG GGAGGAAGGT GTTTCTTGCT GCTGCAACAT TGAGGCCTGA GACGATGTAT 
    GGACAAACAA ACGCGTGGGT GTTGCCAGAT GGGAAATATG GAGCCTTTGA GATCAATGAT 
    ACTGATGTGT TCATTTTATC TGAGAGGGCA GCTCTTAATC TTGCATACCA GAAACTGTCC 
    CGTGTTCCAG AAAAGCCCAG TTGTTTGGCT GAGCTGACCG GCTATGATTT GATCGGTTTG 
    CCCCTCAAGT CCCCATTAGC ATTCAATGAG ATCATATATG CTCTCCCCAT GCTTTCGATT 
    CTCATTGACA AAGGTACTGG AATTGTGACA AGCGTGCCAA GTGATTCACC CGACGATTTC 
    ATGGCTCTCC ATGATTTGAA AACAAAGCCA GTTTTCAGAG CTAAGTTTGG TGTAAAGGAC 
    GAGTGGGTCT TGCCATTTGA GGTCATTCCA ATTATCAATA CCCCAGAATT TGGAGATAAG 
    TCAGCTGAGA AAATCTGCAA AGATTATGGT ATTCAGAGCC AGAATGAGAA AGAGAAGCTT 
    GCTGAGGCCA AGAAACTGAT TTACAGGGGA GGATTCTATG AGGGAACTTT GATTGTTGGA 
    GAATATGCAG GAATGAGAGT CCAGGAAGCT AAGAATCTGA TCAGGAGCAA GCTTCTGGAG 
    CTCGGTCAAG CTGTTGTATA TAGCGAGCCA GAGAAGAAGG TCATCTCAAG ATCAGGGGAT 
    GAGTGTGTGG TGGCTCTGAC TGATCAGTGG TATATCACCT ATGGGGAGCC TGAATGGAAG 
    CAGAAGGCAG AGGAGTGCTT GGCCAGCATG AATCTGTACT CCAATGAGGT GCGGCATGGT 
    TTTGAGCACA CATTGAGCTG GCTGAATCAG TGGGCTTGTT CCCGTTCCTT TGGGCTTGGG 
    ACTCGTCTTC CATGGGATGA AGACTTCCTC GTCGAGTCCT TATCTGATTC GACTCTATAC 
    ATGGCATACT ATACCATATC TCATCTGTTA CAGAAAGGGA ACTTGTATGG TTCTGATACT 
    TCTTCGGTTA AACCAGAGCA AATGACAGAT GAGGTTTGGG ACTTTGTGTT TTGTGGCAGC 
    CCATTCCCCA AATCATCTGA TATTTGCCCC AGTGTTCTGA GGAAGATGAA GCAGGAGTTT 
    GAATATTGGT ATCCATTTGA TGTCAGGACT TCTGGCAAAG ACCTGATTCA GAATCATCTC 
    ACTTTTTGTA TATACAACCA CACAGCAATC CTAGCCAAGC ATCACTGGCC TAGGGGATTC 
    AGATGCAATG GGCACATTAT GCTGAATTCT GAGAAAATGT CCAAGTCCAC TGGAAACTTC 
    ATGACAATAC GCCAAGCTAT CCAGGAATTC TCTGCTGACG CCACACGATT CTCCCTTGCT 
    GATGCTGGAG ATGGAATGGA TGATGCCAAC TTTGTCTCTG AGACGGCAAA TGCTGCAATC 
    TTACGCCTCA CAAAAGAGAT ATCATGGATG CAGGAGGTTA TAGAGGGGGA GTCATCTTTC 
    AGAAAGGGGC CCTCTTCTAC TTATGCTGAT CGCGTGTTTG CCAATGAGAT CAACATTGCA 
    GTCAAAATGA CTGAGAAGAA CTACAGTGAG TTCATGTTCA GGGAAGCTCT CAAGACTGGG 
    TTTTATGATC TCCAAGCTGC CAGGGATGAG TATAGATTTT CTTGTGGTAT GGGGGGCATG 
    AATCGTGATC TGTTATGGCG GTTTATGGAT GTTCAAACTC GTCTTATGAC TCCAATCTGC 
    CCTCATTTTG CTGAATATGT TTGGAAGGAG CTTCTGAGGA AAGAAGGGTT TGTTGTGAAA 
    GCAGGATGGC CTGAAGCTGA TACACTTGAC CTCACTCTCA AGCTTGCCAA CAAGTATCTG 
    CAGGACTCAA TTGTCTCAAT GAGGAAGCTG CTCCAAAAAC AAGTTTCAGG CCCAAAAAGG 
    GCCGACAAAA GCATCTCCTC TAGTGCAGAA AACAGACCAA CTGTCGGACT AATATACATG 
    GCCGAGCAAT ACGACGGATG GAAAGCAGAA TGCTTGAAAA TACTACAAAG CAAGTTCAAC 
    ACAGAAACGA GCAGTTTTGC ACCAGACCAA GAGATCTTGG AGGCATTACA GCAGAGTGAA 
    ATTGGGCAGG AAGGAAACTT TAAGGAGACC CAGAAGCTGT GTATGCCTTT CCTGAGGTTC 
    AAGAAGGATG AGGCCATTGC TGTTGGGCAT CAGGCCTTGG ATCTGAAGCT ACCCTTCGGA 
    GAAGTGGAGG TCCTGGGGGA GAACTTGGAG TTGATCAAGA GGCAGCTTGG TCTAGAGGAG 
    GTTGAAGTTC TGTCTGCTGC AGACCCTGAG GCTATTCAGA AAGCTGGGCA GTATGCCTCA 
    CTGCTAAACC AGAATCCGCC TTCTCCAGGA AACCCAACTG CCATCTTCTT GAGTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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gene@genscript.com

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