LOC100245471 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100245471
Entrez Gene ID	100245471
Full Name	putative E3 ubiquitin-protein ligase LIN-1
Synonyms	GO:0000151 GO:0016567
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002279827.4	XP_002279863.2	putative E3 ubiquitin-protein ligase LIN-1 isoform X2
XM_010666518.2	XP_010664820.1	putative E3 ubiquitin-protein ligase LIN-1 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100245471 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100245471 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa19345	XM_002279827.4 Latest version!	Vitis vinifera putative E3 ubiquitin-protein ligase LIN-1 (LOC100245471), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OVa24871	XM_010666518.2 Latest version!	Vitis vinifera putative E3 ubiquitin-protein ligase LIN-1 (LOC100245471), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100245471 ( XM_002279827.4 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_002279863.2 Vitis vinifera putative E3 ubiquitin-protein ligase LIN-1 (LOC100245471), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OVa19345
Clone ID Related Accession (Same CDS sequence)	XM_002279827.4
Accession Version	XM_002279827.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	putative E3 ubiquitin-protein ligase LIN-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012024.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 22, 2016 this sequence version replaced XM_002279827.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA ACTACAGGTT TGCAATGGAC CAAAAGGATA TCGTTAGATT CCTGGTCACC 
ACGGTTGGAA GTTTCATCCA AGATCAGTTG ATCAATAAAG AACAGAGAGC CCAACACAAA 
GAACAGTGTG CAGAGAGATT AGCAGCTGAA GATGGAAGCT GTGAGAAAGA CACAGAAGTT 
CGATACTCTG ATCAAGCAGT ATTGGCAAAT TTGGATTGGG GGATTGATGC CCTTGAAGAA 
GCTATCAATA CCTCCAACAT GGAAACTAAG CTTGCACGAT TAGACCATGC AGAAAAAATG 
TTACAAGTAT GTGCAATGTT GAACTCTGAC CAGAGAACTG CAGGAGTCCC CAATTTCTAC 
CTCTCTGCTT GGGCCCATCT CAACCTTTCT TACCTGTGGA GACTGCGGGA CAATGTTCAC 
AATTCAGTTC TTCACATTCT CGAGATGTTT ATTGTTGATC CTTTCTTCTC ACGGATTGAT 
TTTGCTCCAG AGCTCTGGAA AGCTCTGTTT CTTCCACATA TGAGTTCAAT TGTTGGGTGG 
TATTCAGAAG CAAGGCACAG GATTGTGATG GAAGTGATTC CTGATTCCAC TGATTTGTCC 
TTTACTGCAG ATTTCGATCA ATTCTTTAAT GAGTCCCTGA TCTTTTCCAT GAGGCCTGAT 
CAAGCAGAGA AAATGCAAAA GCTGGAGCAA CTTTATGGAG AATCATTGGA TGAGAATACA 
CGGCTTTATT CAAAATATTA TATGGATTGC TTGAACTTTG ATTCAAACAC TAGCAAGAGA 
GCGATTCCGA TGTTGCCAAT TGCAGAGCCA CCAATGACTC CAATGCATGA GGTCAGCAGG 
TCAATTCCCG ATTATGTAAA ATTTGGGCCC ATTCTACCTA AGAGTGCTGG GTTTTCTCTA 
ATTCTTAAAG CTAAGGACAA TGCAAGAGAA GCTTGCAGAT TGAATGTCAC TTCCAAATCC 
TCACAGAACC TGGAGGAGTC TGTTATGTGG GACCTTCAAG AGGAAACGAT TGAGGAAAAT 
GAAGATAATT CAGATTATGA GCCTGATAGT GCTTATATGG ATTCTGAGGA CAAAACATAT 
CAAGTAGTAT CTCATAGTAG CATGAAAATG AACATTTATA AGGAAATGGG GCCAAAAGTA 
CAGCCACCAA AAATTAGGAG TCAACTACCT TCTTCGACAA GTTTTTCTTC CACGGACTCC 
CCTAAAACTT CTCTAAAGAT TTCATCTCCA AAATCAGACT CGCATTGTCA TAAAGGTCCC 
ACATCCGTAT TGCGCCTCTT ATCCAGCCGT GCCATGGATT CCACTGTCTC CACCTCCTTG 
CCTGTGTCTC CACGTTTGTA TAAAGATTCC AGCATTAGCT CAGCAGACTC AGATGGTGAA 
GTGATTGAGC TACCAAGAAG CTGCAGGAAA AACCATGGAC ACAATCAAAG TATCAGTCAT 
CAAAATCTGA ATAGACAAGT ATCAGAAAAT AGCTCCCTTA ATGAAAATGA TGAAGGAAGC 
CAGAGCTGCA TTTCTCTCCC ATTATCTGAT AAACTGACTC CTAGGTCAAG GCCGCCTAAA 
GATTTTGTCT GCCCCATCAC AGGGCAGATT TTTAGTGATG CAGTTACCCT TGAAACAGGT 
CAAACATATG AAAGAAAAGC AATTCAAGAA TGGCTGAAAA GAGGAAACAC AACATGTCCC 
ATTACACGGC AACCTCTATC TGCCAGCTCA CTGCCCAAAA CAAACTATGT TTTGAAGAGA 
TTAATCACTA CTTGGAAAGA ACAATATCCT GATGTTGCTC AGGAGTTCTC TTACCCTGAA 
ACACCAAGAA ACTCATTCAG CCCCCCGTCC ACAAAAGAAA TTATGTTGGC ATCTTCTCCA 
TCATGTAATC CCCCCGACCA TAAGAAGTCA GATGATTGTA CTAATCAGAA GTGCAAAAGA 
TTTATGCAAA CAGTGGTATC TACTTCACCA ACCAGTGTGA TATCTCAAGC TGCTACTGAA 
GCGGTTATCA ATGGGTTAAA ACCCTATGTT TTGTGTCTCT GTAATTCAGA TGATTTACAA 
GAGTGTGAAG CAGCTGTGCT GGCAATAGCC AAGATGTGGA AAGACTCAAA GGCTGATCCG 
GGAATTCATT CTTATTTGTC CGAACCAACA ATTGTGAATG GGCTTGTGGA AATACTATCA 
GCTTCCATGA ACAGAGAAGT TCTCAGAACA TCAATTCACA TTCTTTCTGT GCTAATATTT 
GCTGATGAAA GTGTGGGAGA GACTCTTACC AATGTAGATT CTGATTTTGA TTGTCTGGCT 
GCTCTACTAA AGAAAGGTCT TGCAGAGGCT GCTGTTCTTA TATACCAGCT GAGGCCAGCA 
TTCACTCAGC TTTCAGCTCG CAACTTTATA CCCTCTCTAG TACACCTAAT TCTGAACAAA 
AATGACGAAT CGGATAATCT TCTGTTAGTA ATGGAGCCCA AGGATGCTGC TATAGCATTG 
CTTGAGCAGA TTTTGATGGG AGGGGATGAA AACAGCAGAT CCCTCAATGC TATGAGTGTT 
ATCTCTGCAA ACGGGATTCC TGCATTAATC AAGTGTTTGG ATAAGGTAGA AGGAAGGCAG 
GCCATCGTAT CCATCCTTTT ATGTTGTATC CATGCAGATA GAAGTTGCAG GAATTTGATA 
GCAAATAGAA TCGATCTGTC TTCCGTGCTT GAATTATTTC ATACTGGAGA TGATTATGTT 
AGAGGCATAT GCACAAAGTT TCTCTCTGAG CTAGTTCAGT TAAACAGGAG GATATTCTGC 
AACCAGATCT TGAAGATAAT TAAAGATGAA GGAGCTTTTA GTACCATGCA CACATTTCTT 
GTGTATCTAC AAATGGCCCC CATGGAGCAG CAACCTGCAA TTGCTTCTCT TCTTCTTCAG 
CTTGATCTTC TGGTTGAGCC TCGAAAGATG AGCATTTACA GGGAAGAAGC TATAGAGGCA 
CTAGTTGAAG CACTTCACAA AAAAGACTTT CCTCATTCTC AAATCATGGC TCTAGATGCT 
TTATTATCTC TTTCTGGACA TCTGACTACC TCAGGGAAGT CCTACACTGA AGCCTGGTTA 
CTCAAGATCG CAGGATATGA TCAACCTTAC CATGCTTTAA TGAAGTCGGA GAGGCTGAAA 
ATATATGAAA ATGAACTGAC AGAGACAACG GAAGAGGAAG AGAAAGCTGT CACTTCTTGG 
CAAAAAAGAG TAGTTTTTGT TCTTTGCAAC CATGAAAAAG GATCCATCTT TAAAGCCTTG 
GAGGAATGCC TGAAGAGTAA TTCTTTAGAG ATAGCAAAGT CATGCCTTGT TGTTGCCACA 
TGGCTTACAT ACATGCTTTA TCATCTTCCT GACACTGGTG TAAGAAATGT TGCTCACAAG 
TCATTCCTCG AGCCATTCAT CAATGTCCTC CAATCATCAA AGAATCTTGA GGAGAAGATT 
TTGGCAACCC TTGCTTTGAA TTGCTTCCTT AATGATCCAG GTGCATTAGA AGAACTGGGA 
GCATATGCCA AGTGCATGTA TAAAACCTTG AGAAAGCTTA AAAAGAATTC AGTGGTTGTA 
AGTGACATGC TAAAAGCCTT GATAAAGCTG CCTTCTGTTG ATCCAACAGA GTTGTGGTGT 
TGTGATGAAG TGGTTGAGCT AGATTCATGC TCAAACGGTG GGATTCTGTC TTTGCTTCCT 
CTAAAAAGTT GGGTTCTGAG CGGCCATTCT GATGGAACTA TAAAGGTATG GGATGCTGGG 
AAGAGAGACT TAAGGTTGAT TCAAGAAGTT CGTGAACACA CAAAGGCTGT CACATGCCTC 
TATGCCTCAT CTTCAAGTGA CAAGTTATAT AGTGGTTCCT TAGACAAAAC AATTCGGGTC 
TGGACGGTCA AACCAGAAGA AATTCACTGT GTTCAGGTTC ATGATGTGAA GGAGGCGGTA 
TACCAGTTGA CAGCTAATGC CAGTTTTGCA TGCTTCTCTT CTCAAGGAAC TGGAGTCAAT 
GTCTACAGTT GGTCTGGGGT TCCAAAACAT ATAAACTTTA ACAAAAATGT CAAGAGCCTT 
GACATGGCAG AGGATAGACT GTATTGCGGT TGCACTGGTT ACAGCATCCA GGAGGTTGAT 
TTGTGTAAAT CCACTACAAA TACATTTTAC TCTGGTGCAA GAAAATTGCT TGGTAAACAA 
ACAATATATT CCTTGCGGAT TCATGATGGT TTACTCTATG CTGGCGGTTC CTCAGTGGAT 
GGAACTGCAG GGAAGGTGTT CTCCCTCTCG ACCAAGGCCT TAACTGGGTC TTTCTTGACT 
GGATTGGACA TCCAGCGCTT AGCTGTGAAT AGTGACTTTA TATTCACTGC CAGTAAATCT 
GGGATCATTG AGGTGTGGTT TAAGGAAACA GTGACAAGAG TAGCTTCCAT TAAAATCGGT 
GGTCATGGAC ATGCGAAGAT TGCATCCTTG GCATCAGATA CAGATGGAGA AATGCTTTTT 
GCTGGTTTCC TTGATGGCAA GATCCAGGCT TGGGCGCTCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002279863.2
CDS	332..4816
Translation	MAGNYRFAMDQKDIVRFLVTTVGSFIQDQLINKEQRAQHKEQCAERLAAEDGSCEKDTEVRYSDQAVLANLDWGIDALEEAINTSNMETKLARLDHAEKMLQVCAMLNSDQRTAGVPNFYLSAWAHLNLSYLWRLRDNVHNSVLHILEMFIVDPFFSRIDFAPELWKALFLPHMSSIVGWYSEARHRIVMEVIPDSTDLSFTADFDQFFNESLIFSMRPDQAEKMQKLEQLYGESLDENTRLYSKYYMDCLNFDSNTSKRAIPMLPIAEPPMTPMHEVSRSIPDYVKFGPILPKSAGFSLILKAKDNAREACRLNVTSKSSQNLEESVMWDLQEETIEENEDNSDYEPDSAYMDSEDKTYQVVSHSSMKMNIYKEMGPKVQPPKIRSQLPSSTSFSSTDSPKTSLKISSPKSDSHCHKGPTSVLRLLSSRAMDSTVSTSLPVSPRLYKDSSISSADSDGEVIELPRSCRKNHGHNQSISHQNLNRQVSENSSLNENDEGSQSCISLPLSDKLTPRSRPPKDFVCPITGQIFSDAVTLETGQTYERKAIQEWLKRGNTTCPITRQPLSASSLPKTNYVLKRLITTWKEQYPDVAQEFSYPETPRNSFSPPSTKEIMLASSPSCNPPDHKKSDDCTNQKCKRFMQTVVSTSPTSVISQAATEAVINGLKPYVLCLCNSDDLQECEAAVLAIAKMWKDSKADPGIHSYLSEPTIVNGLVEILSASMNREVLRTSIHILSVLIFADESVGETLTNVDSDFDCLAALLKKGLAEAAVLIYQLRPAFTQLSARNFIPSLVHLILNKNDESDNLLLVMEPKDAAIALLEQILMGGDENSRSLNAMSVISANGIPALIKCLDKVEGRQAIVSILLCCIHADRSCRNLIANRIDLSSVLELFHTGDDYVRGICTKFLSELVQLNRRIFCNQILKIIKDEGAFSTMHTFLVYLQMAPMEQQPAIASLLLQLDLLVEPRKMSIYREEAIEALVEALHKKDFPHSQIMALDALLSLSGHLTTSGKSYTEAWLLKIAGYDQPYHALMKSERLKIYENELTETTEEEEKAVTSWQKRVVFVLCNHEKGSIFKALEECLKSNSLEIAKSCLVVATWLTYMLYHLPDTGVRNVAHKSFLEPFINVLQSSKNLEEKILATLALNCFLNDPGALEELGAYAKCMYKTLRKLKKNSVVVSDMLKALIKLPSVDPTELWCCDEVVELDSCSNGGILSLLPLKSWVLSGHSDGTIKVWDAGKRDLRLIQEVREHTKAVTCLYASSSSDKLYSGSLDKTIRVWTVKPEEIHCVQVHDVKEAVYQLTANASFACFSSQGTGVNVYSWSGVPKHINFNKNVKSLDMAEDRLYCGCTGYSIQEVDLCKSTTNTFYSGARKLLGKQTIYSLRIHDGLLYAGGSSVDGTAGKVFSLSTKALTGSFLTGLDIQRLAVNSDFIFTASKSGIIEVWFKETVTRVASIKIGGHGHAKIASLASDTDGEMLFAGFLDGKIQAWALD

Target ORF information:

RefSeq Version	XM_002279827.4
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera putative E3 ubiquitin-protein ligase LIN-1 (LOC100245471), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002279827.4

ORF Insert Sequence:

ATGGCTGGAA ACTACAGGTT TGCAATGGAC CAAAAGGATA TCGTTAGATT CCTGGTCACC 
ACGGTTGGAA GTTTCATCCA AGATCAGTTG ATCAATAAAG AACAGAGAGC CCAACACAAA 
GAACAGTGTG CAGAGAGATT AGCAGCTGAA GATGGAAGCT GTGAGAAAGA CACAGAAGTT 
CGATACTCTG ATCAAGCAGT ATTGGCAAAT TTGGATTGGG GGATTGATGC CCTTGAAGAA 
GCTATCAATA CCTCCAACAT GGAAACTAAG CTTGCACGAT TAGACCATGC AGAAAAAATG 
TTACAAGTAT GTGCAATGTT GAACTCTGAC CAGAGAACTG CAGGAGTCCC CAATTTCTAC 
CTCTCTGCTT GGGCCCATCT CAACCTTTCT TACCTGTGGA GACTGCGGGA CAATGTTCAC 
AATTCAGTTC TTCACATTCT CGAGATGTTT ATTGTTGATC CTTTCTTCTC ACGGATTGAT 
TTTGCTCCAG AGCTCTGGAA AGCTCTGTTT CTTCCACATA TGAGTTCAAT TGTTGGGTGG 
TATTCAGAAG CAAGGCACAG GATTGTGATG GAAGTGATTC CTGATTCCAC TGATTTGTCC 
TTTACTGCAG ATTTCGATCA ATTCTTTAAT GAGTCCCTGA TCTTTTCCAT GAGGCCTGAT 
CAAGCAGAGA AAATGCAAAA GCTGGAGCAA CTTTATGGAG AATCATTGGA TGAGAATACA 
CGGCTTTATT CAAAATATTA TATGGATTGC TTGAACTTTG ATTCAAACAC TAGCAAGAGA 
GCGATTCCGA TGTTGCCAAT TGCAGAGCCA CCAATGACTC CAATGCATGA GGTCAGCAGG 
TCAATTCCCG ATTATGTAAA ATTTGGGCCC ATTCTACCTA AGAGTGCTGG GTTTTCTCTA 
ATTCTTAAAG CTAAGGACAA TGCAAGAGAA GCTTGCAGAT TGAATGTCAC TTCCAAATCC 
TCACAGAACC TGGAGGAGTC TGTTATGTGG GACCTTCAAG AGGAAACGAT TGAGGAAAAT 
GAAGATAATT CAGATTATGA GCCTGATAGT GCTTATATGG ATTCTGAGGA CAAAACATAT 
CAAGTAGTAT CTCATAGTAG CATGAAAATG AACATTTATA AGGAAATGGG GCCAAAAGTA 
CAGCCACCAA AAATTAGGAG TCAACTACCT TCTTCGACAA GTTTTTCTTC CACGGACTCC 
CCTAAAACTT CTCTAAAGAT TTCATCTCCA AAATCAGACT CGCATTGTCA TAAAGGTCCC 
ACATCCGTAT TGCGCCTCTT ATCCAGCCGT GCCATGGATT CCACTGTCTC CACCTCCTTG 
CCTGTGTCTC CACGTTTGTA TAAAGATTCC AGCATTAGCT CAGCAGACTC AGATGGTGAA 
GTGATTGAGC TACCAAGAAG CTGCAGGAAA AACCATGGAC ACAATCAAAG TATCAGTCAT 
CAAAATCTGA ATAGACAAGT ATCAGAAAAT AGCTCCCTTA ATGAAAATGA TGAAGGAAGC 
CAGAGCTGCA TTTCTCTCCC ATTATCTGAT AAACTGACTC CTAGGTCAAG GCCGCCTAAA 
GATTTTGTCT GCCCCATCAC AGGGCAGATT TTTAGTGATG CAGTTACCCT TGAAACAGGT 
CAAACATATG AAAGAAAAGC AATTCAAGAA TGGCTGAAAA GAGGAAACAC AACATGTCCC 
ATTACACGGC AACCTCTATC TGCCAGCTCA CTGCCCAAAA CAAACTATGT TTTGAAGAGA 
TTAATCACTA CTTGGAAAGA ACAATATCCT GATGTTGCTC AGGAGTTCTC TTACCCTGAA 
ACACCAAGAA ACTCATTCAG CCCCCCGTCC ACAAAAGAAA TTATGTTGGC ATCTTCTCCA 
TCATGTAATC CCCCCGACCA TAAGAAGTCA GATGATTGTA CTAATCAGAA GTGCAAAAGA 
TTTATGCAAA CAGTGGTATC TACTTCACCA ACCAGTGTGA TATCTCAAGC TGCTACTGAA 
GCGGTTATCA ATGGGTTAAA ACCCTATGTT TTGTGTCTCT GTAATTCAGA TGATTTACAA 
GAGTGTGAAG CAGCTGTGCT GGCAATAGCC AAGATGTGGA AAGACTCAAA GGCTGATCCG 
GGAATTCATT CTTATTTGTC CGAACCAACA ATTGTGAATG GGCTTGTGGA AATACTATCA 
GCTTCCATGA ACAGAGAAGT TCTCAGAACA TCAATTCACA TTCTTTCTGT GCTAATATTT 
GCTGATGAAA GTGTGGGAGA GACTCTTACC AATGTAGATT CTGATTTTGA TTGTCTGGCT 
GCTCTACTAA AGAAAGGTCT TGCAGAGGCT GCTGTTCTTA TATACCAGCT GAGGCCAGCA 
TTCACTCAGC TTTCAGCTCG CAACTTTATA CCCTCTCTAG TACACCTAAT TCTGAACAAA 
AATGACGAAT CGGATAATCT TCTGTTAGTA ATGGAGCCCA AGGATGCTGC TATAGCATTG 
CTTGAGCAGA TTTTGATGGG AGGGGATGAA AACAGCAGAT CCCTCAATGC TATGAGTGTT 
ATCTCTGCAA ACGGGATTCC TGCATTAATC AAGTGTTTGG ATAAGGTAGA AGGAAGGCAG 
GCCATCGTAT CCATCCTTTT ATGTTGTATC CATGCAGATA GAAGTTGCAG GAATTTGATA 
GCAAATAGAA TCGATCTGTC TTCCGTGCTT GAATTATTTC ATACTGGAGA TGATTATGTT 
AGAGGCATAT GCACAAAGTT TCTCTCTGAG CTAGTTCAGT TAAACAGGAG GATATTCTGC 
AACCAGATCT TGAAGATAAT TAAAGATGAA GGAGCTTTTA GTACCATGCA CACATTTCTT 
GTGTATCTAC AAATGGCCCC CATGGAGCAG CAACCTGCAA TTGCTTCTCT TCTTCTTCAG 
CTTGATCTTC TGGTTGAGCC TCGAAAGATG AGCATTTACA GGGAAGAAGC TATAGAGGCA 
CTAGTTGAAG CACTTCACAA AAAAGACTTT CCTCATTCTC AAATCATGGC TCTAGATGCT 
TTATTATCTC TTTCTGGACA TCTGACTACC TCAGGGAAGT CCTACACTGA AGCCTGGTTA 
CTCAAGATCG CAGGATATGA TCAACCTTAC CATGCTTTAA TGAAGTCGGA GAGGCTGAAA 
ATATATGAAA ATGAACTGAC AGAGACAACG GAAGAGGAAG AGAAAGCTGT CACTTCTTGG 
CAAAAAAGAG TAGTTTTTGT TCTTTGCAAC CATGAAAAAG GATCCATCTT TAAAGCCTTG 
GAGGAATGCC TGAAGAGTAA TTCTTTAGAG ATAGCAAAGT CATGCCTTGT TGTTGCCACA 
TGGCTTACAT ACATGCTTTA TCATCTTCCT GACACTGGTG TAAGAAATGT TGCTCACAAG 
TCATTCCTCG AGCCATTCAT CAATGTCCTC CAATCATCAA AGAATCTTGA GGAGAAGATT 
TTGGCAACCC TTGCTTTGAA TTGCTTCCTT AATGATCCAG GTGCATTAGA AGAACTGGGA 
GCATATGCCA AGTGCATGTA TAAAACCTTG AGAAAGCTTA AAAAGAATTC AGTGGTTGTA 
AGTGACATGC TAAAAGCCTT GATAAAGCTG CCTTCTGTTG ATCCAACAGA GTTGTGGTGT 
TGTGATGAAG TGGTTGAGCT AGATTCATGC TCAAACGGTG GGATTCTGTC TTTGCTTCCT 
CTAAAAAGTT GGGTTCTGAG CGGCCATTCT GATGGAACTA TAAAGGTATG GGATGCTGGG 
AAGAGAGACT TAAGGTTGAT TCAAGAAGTT CGTGAACACA CAAAGGCTGT CACATGCCTC 
TATGCCTCAT CTTCAAGTGA CAAGTTATAT AGTGGTTCCT TAGACAAAAC AATTCGGGTC 
TGGACGGTCA AACCAGAAGA AATTCACTGT GTTCAGGTTC ATGATGTGAA GGAGGCGGTA 
TACCAGTTGA CAGCTAATGC CAGTTTTGCA TGCTTCTCTT CTCAAGGAAC TGGAGTCAAT 
GTCTACAGTT GGTCTGGGGT TCCAAAACAT ATAAACTTTA ACAAAAATGT CAAGAGCCTT 
GACATGGCAG AGGATAGACT GTATTGCGGT TGCACTGGTT ACAGCATCCA GGAGGTTGAT 
TTGTGTAAAT CCACTACAAA TACATTTTAC TCTGGTGCAA GAAAATTGCT TGGTAAACAA 
ACAATATATT CCTTGCGGAT TCATGATGGT TTACTCTATG CTGGCGGTTC CTCAGTGGAT 
GGAACTGCAG GGAAGGTGTT CTCCCTCTCG ACCAAGGCCT TAACTGGGTC TTTCTTGACT 
GGATTGGACA TCCAGCGCTT AGCTGTGAAT AGTGACTTTA TATTCACTGC CAGTAAATCT 
GGGATCATTG AGGTGTGGTT TAAGGAAACA GTGACAAGAG TAGCTTCCAT TAAAATCGGT 
GGTCATGGAC ATGCGAAGAT TGCATCCTTG GCATCAGATA CAGATGGAGA AATGCTTTTT 
GCTGGTTTCC TTGATGGCAA GATCCAGGCT TGGGCGCTCG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100245471 ( XM_010666518.2 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_010664820.1 Vitis vinifera putative E3 ubiquitin-protein ligase LIN-1 (LOC100245471), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OVa24871
Clone ID Related Accession (Same CDS sequence)	XM_010666518.2
Accession Version	XM_010666518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	putative E3 ubiquitin-protein ligase LIN-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012024.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 22, 2016 this sequence version replaced XM_010666518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA ACTACAGGTT TGCAATGGAC CAAAAGGATA TCGTTAGATT CCTGGTCACC 
ACGGTTGGAA GTTTCATCCA AGATCAGTTG ATCAATAAAG AACAGAGAGC CCAACACAAA 
GAACAGTGTG CAGAGAGATT AGCAGCTGAA GATGGAAGCT GTGAGAAAGA CACAGAAGTT 
CGATACTCTG ATCAAGCAGT ATTGGCAAAT TTGGATTGGG GGATTGATGC CCTTGAAGAA 
GCTATCAATA CCTCCAACAT GGAAACTAAG CTTGCACGAT TAGACCATGC AGAAAAAATG 
TTACAAGTAT GTGCAATGTT GAACTCTGAC CAGAGAACTG CAGGAGTCCC CAATTTCTAC 
CTCTCTGCTT GGGCCCATCT CAACCTTTCT TACCTGTGGA GACTGCGGGA CAATGTTCAC 
AATTCAGTTC TTCACATTCT CGAGATGTTT ATTGTTGATC CTTTCTTCTC ACGGATTGAT 
TTTGCTCCAG AGCTCTGGAA AGCTCTGTTT CTTCCACATA TGAGTTCAAT TGTTGGGTGG 
TATTCAGAAG CAAGGCACAG GATTGTGATG GAAGTGATTC CTGATTCCAC TGATTTGTCC 
TTTACTGCAG ATTTCGATCA ATTCTTTAAT GAGTCCCTGA TCTTTTCCAT GAGGCCTGAT 
CAAGCAGAGA AAATGCAAAA GCTGGAGCAA CTTTATGGAG AATCATTGGA TGAGAATACA 
CGGCTTTATT CAAAATATTA TATGGATTGC TTGAACTTTG ATTCAAACAC TAGCAAGAGA 
GCGATTCCGA TGTTGCCAAT TGCAGAGCCA CCAATGACTC CAATGCATGA GGTCAGCAGG 
TCAATTCCCG ATTATGTAAA ATTTGGGCCC ATTCTACCTA AGAGTGCTGG GTTTTCTCTA 
ATTCTTAAAG CTAAGGACAA TGCAAGAGAA GCTTGCAGAT TGAATGTCAC TTCCAAATCC 
TCACAGAACC TGGAGGAGTC TGTTATGTGG GACCTTCAAG AGGAAACGAT TGAGGAAAAT 
GAAGATAATT CAGATTATGA GCCTGATAGT GCTTATATGG ATTCTGAGGA CAAAACATAT 
CAAGTAGTAT CTCATAGTAG CATGAAAATG AACATTTATA AGGAAATGGG GCCAAAAGTA 
CAGCCACCAA AAATTAGGAG TCAACTACCT TCTTCGACAA GTTTTTCTTC CACGGACTCC 
CCTAAAACTT CTCTAAAGAT TTCATCTCCA AAATCAGACT CGCATTGTCA TAAAGGTCCC 
ACATCCGTAT TGCGCCTCTT ATCCAGCCGT GCCATGGATT CCACTGTCTC CACCTCCTTG 
CCTGTGTCTC CACGTTTGTA TAAAGATTCC AGCATTAGCT CAGCAGACTC AGATGGTGAA 
GTGATTGAGC TACCAAGAAG CTGCAGGAAA AACCATGGAC ACAATCAAAG TATCAGTCAT 
CAAAATCTGA ATAGACAAGT ATCAGAAAAT AGCTCCCTTA ATGAAAATGA TGAAGGAAGC 
CAGAGCTGCA TTTCTCTCCC ATTATCTGAT AAACTGACTC CTAGGTCAAG GCCGCCTAAA 
GATTTTGTCT GCCCCATCAC AGGGCAGATT TTTAGTGATG CAGTTACCCT TGAAACAGGT 
CAAACATATG AAAGAAAAGC AATTCAAGAA TGGCTGAAAA GAGGAAACAC AACATGTCCC 
ATTACACGGC AACCTCTATC TGCCAGCTCA CTGCCCAAAA CAAACTATGT TTTGAAGAGA 
TTAATCACTA CTTGGAAAGA ACAATATCCT GATGTTGCTC AGGAGTTCTC TTACCCTGAA 
ACACCAAGAA ACTCATTCAG CCCCCCGTCC ACAAAAGAAA TTATGTTGGC ATCTTCTCCA 
TCATGTAATC CCCCCGACCA TAAGAAGTCA GATGATTGTA CTAATCAGAA GTGCAAAAGA 
TTTATGCAAA CAGTGGTATC TACTTCACCA ACCAGTGTGA TATCTCAAGC TGCTACTGAA 
GCGGTTATCA ATGGGTTAAA ACCCTATGTT TTGTGTCTCT GTAATTCAGA TGATTTACAA 
GAGTGTGAAG CAGCTGTGCT GGCAATAGCC AAGATGTGGA AAGACTCAAA GGCTGATCCG 
GGAATTCATT CTTATTTGTC CGAACCAACA ATTGTGAATG GGCTTGTGGA AATACTATCA 
GCTTCCATGA ACAGAGAAGT TCTCAGAACA TCAATTCACA TTCTTTCTGT GCTAATATTT 
GCTGATGAAA GTGTGGGAGA GACTCTTACC AATGTAGATT CTGATTTTGA TTGTCTGGCT 
GCTCTACTAA AGAAAGGTCT TGCAGAGGCT GCTGTTCTTA TATACCAGCT GAGGCCAGCA 
TTCACTCAGC TTTCAGCTCG CAACTTTATA CCCTCTCTAG TACACCTAAT TCTGAACAAA 
AATGACGAAT CGGATAATCT TCTGTTAGTA ATGGAGCCCA AGGATGCTGC TATAGCATTG 
CTTGAGCAGA TTTTGATGGG AGGGGATGAA AACAGCAGAT CCCTCAATGC TATGAGTGTT 
ATCTCTGCAA ACGGGATTCC TGCATTAATC AAGTGTTTGG ATAAGGTAGA AGGAAGGCAG 
GCCATCGTAT CCATCCTTTT ATGTTGTATC CATGCAGATA GAAGTTGCAG GAATTTGATA 
GCAAATAGAA TCGATCTGTC TTCCGTGCTT GAATTATTTC ATACTGGAGA TGATTATGTT 
AGAGGCATAT GCACAAAGTT TCTCTCTGAG CTAGTTCAGT TAAACAGGAG GATATTCTGC 
AACCAGATCT TGAAGATAAT TAAAGATGAA GGAGCTTTTA GTACCATGCA CACATTTCTT 
GTGTATCTAC AAATGGCCCC CATGGAGCAG CAACCTGCAA TTGCTTCTCT TCTTCTTCAG 
CTTGATCTTC TGGTTGAGCC TCGAAAGATG AGCATTTACA GGGAAGAAGC TATAGAGGCA 
CTAGTTGAAG CACTTCACAA AAAAGACTTT CCTCATTCTC AAATCATGGC TCTAGATGCT 
TTATTATCTC TTTCTGGACA TCTGACTACC TCAGGGAAGT CCTACACTGA AGCCTGGTTA 
CTCAAGATCG CAGGATATGA TCAACCTTAC CATGCTTTAA TGAAGTCGGA GAGGCTGAAA 
ATATATGAAA ATGAACTGAC AGAGACAACG GAAGAGGAAG AGAAAGCTGT CACTTCTTGG 
CAAAAAAGAG TAGTTTTTGT TCTTTGCAAC CATGAAAAAG GATCCATCTT TAAAGCCTTG 
GAGGAATGCC TGAAGAGTAA TTCTTTAGAG ATAGCAAAGT CATGCCTTGT TGTTGCCACA 
TGGCTTACAT ACATGCTTTA TCATCTTCCT GACACTGGTG TAAGAAATGT TGCTCACAAG 
TCATTCCTCG AGCCATTCAT CAATGTCCTC CAATCATCAA AGAATCTTGA GGAGAAGATT 
TTGGCAACCC TTGCTTTGAA TTGCTTCCTT AATGATCCAG GTGCATTAGA AGAACTGGGA 
GCATATGCCA AGTGCATGTA TAAAACCTTG AGAAAGCTTA AAAAGAATTC AGTGGTTGTA 
AGTGACATGC TAAAAGCCTT GATAAAGCTG CCTTCTGTTG ATCCAACAGA GTTGTGGTGT 
TGTGATGAAG TGGTTGAGCT AGATTCATGC TCAAACGGTG GGATTCTGTC TTTGCTTCCT 
CTAAAAAGTT GGGTTCTGAG CGGCCATTCT GATGGAACTA TAAAGGTATG GGATGCTGGG 
AAGAGAGACT TAAGGTTGAT TCAAGAAGTT CGTGAACACA CAAAGGCTGT CACATGCCTC 
TATGCCTCAT CTTCAAGTGA CAAGTTATAT AGTGGTTCCT TAGACAAAAC AATTCGGGTC 
TGGACGGTCA AACCAGAAGA AATTCACTGT GTTCAGGTTC ATGATGTGAA GGAGGCGGTA 
TACCAGTTGA CAGCTAATGC CAGTTTTGCA TGCTTCTCTT CTCAAGGAAC TGGAGTCAAT 
GTCTACAGTT GGTCTGGGGT TCCAAAACAT ATAAACTTTA ACAAAAATGT CAAGAGCCTT 
GACATGGCAG AGGATAGACT GTATTGCGGT TGCACTGGTT ACAGCATCCA GGAGGTTGAT 
TTGTGTAAAT CCACTACAAA TACATTTTAC TCTGGTGCAA GAAAATTGCT TGGTAAACAA 
ACAATATATT CCTTGCGGAT TCATGATGGT TTACTCTATG CTGGCGGTTC CTCAGTGGAT 
GGAACTGCAG GGAAGGTGTT CTCCCTCTCG ACCAAGGCCT TAACTGGGTC TTTCTTGACT 
GGATTGGACA TCCAGCGCTT AGCTGTGAAT AGTGACTTTA TATTCACTGC CAGTAAATCT 
GGGATCATTG AGGTGTGGTT TAAGGAAACA GTGACAAGAG TAGCTTCCAT TAAAATCGGT 
GGTCATGGAC ATGCGAAGAT TGCATCCTTG GCATCAGATA CAGATGGAGA AATGCTTTTT 
GCTGGTTTCC TTGATGGCAA GATCCAGGTT TGTACACAAA GACATCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010664820.1
CDS	332..4822
Translation	MAGNYRFAMDQKDIVRFLVTTVGSFIQDQLINKEQRAQHKEQCAERLAAEDGSCEKDTEVRYSDQAVLANLDWGIDALEEAINTSNMETKLARLDHAEKMLQVCAMLNSDQRTAGVPNFYLSAWAHLNLSYLWRLRDNVHNSVLHILEMFIVDPFFSRIDFAPELWKALFLPHMSSIVGWYSEARHRIVMEVIPDSTDLSFTADFDQFFNESLIFSMRPDQAEKMQKLEQLYGESLDENTRLYSKYYMDCLNFDSNTSKRAIPMLPIAEPPMTPMHEVSRSIPDYVKFGPILPKSAGFSLILKAKDNAREACRLNVTSKSSQNLEESVMWDLQEETIEENEDNSDYEPDSAYMDSEDKTYQVVSHSSMKMNIYKEMGPKVQPPKIRSQLPSSTSFSSTDSPKTSLKISSPKSDSHCHKGPTSVLRLLSSRAMDSTVSTSLPVSPRLYKDSSISSADSDGEVIELPRSCRKNHGHNQSISHQNLNRQVSENSSLNENDEGSQSCISLPLSDKLTPRSRPPKDFVCPITGQIFSDAVTLETGQTYERKAIQEWLKRGNTTCPITRQPLSASSLPKTNYVLKRLITTWKEQYPDVAQEFSYPETPRNSFSPPSTKEIMLASSPSCNPPDHKKSDDCTNQKCKRFMQTVVSTSPTSVISQAATEAVINGLKPYVLCLCNSDDLQECEAAVLAIAKMWKDSKADPGIHSYLSEPTIVNGLVEILSASMNREVLRTSIHILSVLIFADESVGETLTNVDSDFDCLAALLKKGLAEAAVLIYQLRPAFTQLSARNFIPSLVHLILNKNDESDNLLLVMEPKDAAIALLEQILMGGDENSRSLNAMSVISANGIPALIKCLDKVEGRQAIVSILLCCIHADRSCRNLIANRIDLSSVLELFHTGDDYVRGICTKFLSELVQLNRRIFCNQILKIIKDEGAFSTMHTFLVYLQMAPMEQQPAIASLLLQLDLLVEPRKMSIYREEAIEALVEALHKKDFPHSQIMALDALLSLSGHLTTSGKSYTEAWLLKIAGYDQPYHALMKSERLKIYENELTETTEEEEKAVTSWQKRVVFVLCNHEKGSIFKALEECLKSNSLEIAKSCLVVATWLTYMLYHLPDTGVRNVAHKSFLEPFINVLQSSKNLEEKILATLALNCFLNDPGALEELGAYAKCMYKTLRKLKKNSVVVSDMLKALIKLPSVDPTELWCCDEVVELDSCSNGGILSLLPLKSWVLSGHSDGTIKVWDAGKRDLRLIQEVREHTKAVTCLYASSSSDKLYSGSLDKTIRVWTVKPEEIHCVQVHDVKEAVYQLTANASFACFSSQGTGVNVYSWSGVPKHINFNKNVKSLDMAEDRLYCGCTGYSIQEVDLCKSTTNTFYSGARKLLGKQTIYSLRIHDGLLYAGGSSVDGTAGKVFSLSTKALTGSFLTGLDIQRLAVNSDFIFTASKSGIIEVWFKETVTRVASIKIGGHGHAKIASLASDTDGEMLFAGFLDGKIQVCTQRHP

Target ORF information:

RefSeq Version	XM_010666518.2
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera putative E3 ubiquitin-protein ligase LIN-1 (LOC100245471), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010666518.2

ORF Insert Sequence:

ATGGCTGGAA ACTACAGGTT TGCAATGGAC CAAAAGGATA TCGTTAGATT CCTGGTCACC 
ACGGTTGGAA GTTTCATCCA AGATCAGTTG ATCAATAAAG AACAGAGAGC CCAACACAAA 
GAACAGTGTG CAGAGAGATT AGCAGCTGAA GATGGAAGCT GTGAGAAAGA CACAGAAGTT 
CGATACTCTG ATCAAGCAGT ATTGGCAAAT TTGGATTGGG GGATTGATGC CCTTGAAGAA 
GCTATCAATA CCTCCAACAT GGAAACTAAG CTTGCACGAT TAGACCATGC AGAAAAAATG 
TTACAAGTAT GTGCAATGTT GAACTCTGAC CAGAGAACTG CAGGAGTCCC CAATTTCTAC 
CTCTCTGCTT GGGCCCATCT CAACCTTTCT TACCTGTGGA GACTGCGGGA CAATGTTCAC 
AATTCAGTTC TTCACATTCT CGAGATGTTT ATTGTTGATC CTTTCTTCTC ACGGATTGAT 
TTTGCTCCAG AGCTCTGGAA AGCTCTGTTT CTTCCACATA TGAGTTCAAT TGTTGGGTGG 
TATTCAGAAG CAAGGCACAG GATTGTGATG GAAGTGATTC CTGATTCCAC TGATTTGTCC 
TTTACTGCAG ATTTCGATCA ATTCTTTAAT GAGTCCCTGA TCTTTTCCAT GAGGCCTGAT 
CAAGCAGAGA AAATGCAAAA GCTGGAGCAA CTTTATGGAG AATCATTGGA TGAGAATACA 
CGGCTTTATT CAAAATATTA TATGGATTGC TTGAACTTTG ATTCAAACAC TAGCAAGAGA 
GCGATTCCGA TGTTGCCAAT TGCAGAGCCA CCAATGACTC CAATGCATGA GGTCAGCAGG 
TCAATTCCCG ATTATGTAAA ATTTGGGCCC ATTCTACCTA AGAGTGCTGG GTTTTCTCTA 
ATTCTTAAAG CTAAGGACAA TGCAAGAGAA GCTTGCAGAT TGAATGTCAC TTCCAAATCC 
TCACAGAACC TGGAGGAGTC TGTTATGTGG GACCTTCAAG AGGAAACGAT TGAGGAAAAT 
GAAGATAATT CAGATTATGA GCCTGATAGT GCTTATATGG ATTCTGAGGA CAAAACATAT 
CAAGTAGTAT CTCATAGTAG CATGAAAATG AACATTTATA AGGAAATGGG GCCAAAAGTA 
CAGCCACCAA AAATTAGGAG TCAACTACCT TCTTCGACAA GTTTTTCTTC CACGGACTCC 
CCTAAAACTT CTCTAAAGAT TTCATCTCCA AAATCAGACT CGCATTGTCA TAAAGGTCCC 
ACATCCGTAT TGCGCCTCTT ATCCAGCCGT GCCATGGATT CCACTGTCTC CACCTCCTTG 
CCTGTGTCTC CACGTTTGTA TAAAGATTCC AGCATTAGCT CAGCAGACTC AGATGGTGAA 
GTGATTGAGC TACCAAGAAG CTGCAGGAAA AACCATGGAC ACAATCAAAG TATCAGTCAT 
CAAAATCTGA ATAGACAAGT ATCAGAAAAT AGCTCCCTTA ATGAAAATGA TGAAGGAAGC 
CAGAGCTGCA TTTCTCTCCC ATTATCTGAT AAACTGACTC CTAGGTCAAG GCCGCCTAAA 
GATTTTGTCT GCCCCATCAC AGGGCAGATT TTTAGTGATG CAGTTACCCT TGAAACAGGT 
CAAACATATG AAAGAAAAGC AATTCAAGAA TGGCTGAAAA GAGGAAACAC AACATGTCCC 
ATTACACGGC AACCTCTATC TGCCAGCTCA CTGCCCAAAA CAAACTATGT TTTGAAGAGA 
TTAATCACTA CTTGGAAAGA ACAATATCCT GATGTTGCTC AGGAGTTCTC TTACCCTGAA 
ACACCAAGAA ACTCATTCAG CCCCCCGTCC ACAAAAGAAA TTATGTTGGC ATCTTCTCCA 
TCATGTAATC CCCCCGACCA TAAGAAGTCA GATGATTGTA CTAATCAGAA GTGCAAAAGA 
TTTATGCAAA CAGTGGTATC TACTTCACCA ACCAGTGTGA TATCTCAAGC TGCTACTGAA 
GCGGTTATCA ATGGGTTAAA ACCCTATGTT TTGTGTCTCT GTAATTCAGA TGATTTACAA 
GAGTGTGAAG CAGCTGTGCT GGCAATAGCC AAGATGTGGA AAGACTCAAA GGCTGATCCG 
GGAATTCATT CTTATTTGTC CGAACCAACA ATTGTGAATG GGCTTGTGGA AATACTATCA 
GCTTCCATGA ACAGAGAAGT TCTCAGAACA TCAATTCACA TTCTTTCTGT GCTAATATTT 
GCTGATGAAA GTGTGGGAGA GACTCTTACC AATGTAGATT CTGATTTTGA TTGTCTGGCT 
GCTCTACTAA AGAAAGGTCT TGCAGAGGCT GCTGTTCTTA TATACCAGCT GAGGCCAGCA 
TTCACTCAGC TTTCAGCTCG CAACTTTATA CCCTCTCTAG TACACCTAAT TCTGAACAAA 
AATGACGAAT CGGATAATCT TCTGTTAGTA ATGGAGCCCA AGGATGCTGC TATAGCATTG 
CTTGAGCAGA TTTTGATGGG AGGGGATGAA AACAGCAGAT CCCTCAATGC TATGAGTGTT 
ATCTCTGCAA ACGGGATTCC TGCATTAATC AAGTGTTTGG ATAAGGTAGA AGGAAGGCAG 
GCCATCGTAT CCATCCTTTT ATGTTGTATC CATGCAGATA GAAGTTGCAG GAATTTGATA 
GCAAATAGAA TCGATCTGTC TTCCGTGCTT GAATTATTTC ATACTGGAGA TGATTATGTT 
AGAGGCATAT GCACAAAGTT TCTCTCTGAG CTAGTTCAGT TAAACAGGAG GATATTCTGC 
AACCAGATCT TGAAGATAAT TAAAGATGAA GGAGCTTTTA GTACCATGCA CACATTTCTT 
GTGTATCTAC AAATGGCCCC CATGGAGCAG CAACCTGCAA TTGCTTCTCT TCTTCTTCAG 
CTTGATCTTC TGGTTGAGCC TCGAAAGATG AGCATTTACA GGGAAGAAGC TATAGAGGCA 
CTAGTTGAAG CACTTCACAA AAAAGACTTT CCTCATTCTC AAATCATGGC TCTAGATGCT 
TTATTATCTC TTTCTGGACA TCTGACTACC TCAGGGAAGT CCTACACTGA AGCCTGGTTA 
CTCAAGATCG CAGGATATGA TCAACCTTAC CATGCTTTAA TGAAGTCGGA GAGGCTGAAA 
ATATATGAAA ATGAACTGAC AGAGACAACG GAAGAGGAAG AGAAAGCTGT CACTTCTTGG 
CAAAAAAGAG TAGTTTTTGT TCTTTGCAAC CATGAAAAAG GATCCATCTT TAAAGCCTTG 
GAGGAATGCC TGAAGAGTAA TTCTTTAGAG ATAGCAAAGT CATGCCTTGT TGTTGCCACA 
TGGCTTACAT ACATGCTTTA TCATCTTCCT GACACTGGTG TAAGAAATGT TGCTCACAAG 
TCATTCCTCG AGCCATTCAT CAATGTCCTC CAATCATCAA AGAATCTTGA GGAGAAGATT 
TTGGCAACCC TTGCTTTGAA TTGCTTCCTT AATGATCCAG GTGCATTAGA AGAACTGGGA 
GCATATGCCA AGTGCATGTA TAAAACCTTG AGAAAGCTTA AAAAGAATTC AGTGGTTGTA 
AGTGACATGC TAAAAGCCTT GATAAAGCTG CCTTCTGTTG ATCCAACAGA GTTGTGGTGT 
TGTGATGAAG TGGTTGAGCT AGATTCATGC TCAAACGGTG GGATTCTGTC TTTGCTTCCT 
CTAAAAAGTT GGGTTCTGAG CGGCCATTCT GATGGAACTA TAAAGGTATG GGATGCTGGG 
AAGAGAGACT TAAGGTTGAT TCAAGAAGTT CGTGAACACA CAAAGGCTGT CACATGCCTC 
TATGCCTCAT CTTCAAGTGA CAAGTTATAT AGTGGTTCCT TAGACAAAAC AATTCGGGTC 
TGGACGGTCA AACCAGAAGA AATTCACTGT GTTCAGGTTC ATGATGTGAA GGAGGCGGTA 
TACCAGTTGA CAGCTAATGC CAGTTTTGCA TGCTTCTCTT CTCAAGGAAC TGGAGTCAAT 
GTCTACAGTT GGTCTGGGGT TCCAAAACAT ATAAACTTTA ACAAAAATGT CAAGAGCCTT 
GACATGGCAG AGGATAGACT GTATTGCGGT TGCACTGGTT ACAGCATCCA GGAGGTTGAT 
TTGTGTAAAT CCACTACAAA TACATTTTAC TCTGGTGCAA GAAAATTGCT TGGTAAACAA 
ACAATATATT CCTTGCGGAT TCATGATGGT TTACTCTATG CTGGCGGTTC CTCAGTGGAT 
GGAACTGCAG GGAAGGTGTT CTCCCTCTCG ACCAAGGCCT TAACTGGGTC TTTCTTGACT 
GGATTGGACA TCCAGCGCTT AGCTGTGAAT AGTGACTTTA TATTCACTGC CAGTAAATCT 
GGGATCATTG AGGTGTGGTT TAAGGAAACA GTGACAAGAG TAGCTTCCAT TAAAATCGGT 
GGTCATGGAC ATGCGAAGAT TGCATCCTTG GCATCAGATA CAGATGGAGA AATGCTTTTT 
GCTGGTTTCC TTGATGGCAA GATCCAGGTT TGTACACAAA GACATCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100245471 cDNA ORF clone, Vitis vinifera(wine grape)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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