LOC100242719 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100242719
Entrez Gene ID	100242719
Full Name	nucleolar protein 6
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010650840.2	XP_010649142.1	nucleolar protein 6 isoform X1
XM_010650841.2	XP_010649143.1	nucleolar protein 6 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100242719 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100242719 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa23800	XM_010650840.2 Latest version!	Vitis vinifera nucleolar protein 6 (LOC100242719), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OVa23801	XM_010650841.2 Latest version!	Vitis vinifera nucleolar protein 6 (LOC100242719), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100242719 ( XM_010650840.2 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_010649142.1 Vitis vinifera nucleolar protein 6 (LOC100242719), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OVa23800
Clone ID Related Accession (Same CDS sequence)	XM_010650840.2
Accession Version	XM_010650840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479916800000
Organism	Vitis vinifera(wine grape)
Product	nucleolar protein 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012010.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 25, 2016 this sequence version replaced XM_010650840.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG ATACGATTAT GGAACCGATG GATTTGAAGG TGCGAGAGCT GTTGAAGGAG 
GTACAGCTCG ACTACTCTTC TGCAACAACA AAGCTCGTCG ACGACACCGT TTCAGCCATT 
AAACAAGCCA TCGATACAAT CCCTGAAGAC TTGAAGGTTA CAGCAGATTT TGCTCCACAA 
TTTGTCAGAG ACATTGGCGC TGATAAAGTT GAATTCAACT TCAAGAAGCC AAAATTATTC 
GAAATTGGTG GTAGTTATTC AATACGCTGT GTGGCAAAGC CTGATGTAGA TATTGATCTT 
TTTGTTAGGT TGCCAAAGGA GTGTTTCCAT GAGAAGGACT ATTTGAATCA CCGTTACCAT 
GCAAAAAGGT TCCTCTATCT CTGCATAATC AAGAAGTATT TGAACTCTTC TTCCTTCATT 
CGAAAGGTTG AATGGTCCAC CCTACAAAAT GAGGCTCGAA AACCAGTATT GGTTGTCTAT 
CCAGCTATGG AGCTTGCTGA AGTTCCTGGG CTTTCTGTGA GAATAATACC TACGGCAACA 
TCCCTGTTTA GTATTTTGAA GTTAAACTTG AAGCGGAACA ATGTTTGTTC TTTGAAGCAA 
GATGAGAGTA CTCCTCAGGC TACACCAAAA TACAATAGTA GTATTTTGGA AGACATGTTT 
CTAGAGGACA ATGCGGAATT TGTGAAGCGA ACTTTTCTTG GTTGGAAGGA GTTGGGGGAG 
GCTTTAATTT TGCTTAAGGT ATGGGCTCGG CAGAGAAGTT CGATATATGC TTATGATTGC 
TTAAATGGAT TTCTGATCTC TGTCATAATG TCATACCTTG CAACGGATTC TGGAAGGAAT 
CTCATTAACA ATTCAATGAA GCCAATGCAG ATATTTCGTG TGACCTTGGA TTTCATTGCC 
ACATCTAAGC TATGGAATAC TGGTCTTTAT TTCAAGTCTC AAAGCCTACT GAATATTTCA 
AAGGAGGAGT TGCTGGAAAG GAAGCAATAT TTAAGATTAT TTCCTGTAGT TATATCTGAG 
TCACTTGCTC ATTTTAATTT GGCGTTCCGG ATTACAGGAG GTGGCTTTTT GGAGCTCCAA 
GATGAGGCTG TTTTGACCCT TAGTTGCATA GGCAAATGTA AAGATGGTGG GTTTGAGGAG 
CTTTTTATGA CTAAGATTGA CTATCCAGCT AAATATGACT ACTGCATGAG ATTGAATTTG 
AAAGGAAATA GTGATGTTTA TGCATTGGGG TTTTGCTTGG ATGAGGAATG TTGGAGATCC 
TTTGAGCAGA AAGTTCATTT TCTTCTGTGT CAAGGATTGA GTGATAGAGC AAAGTTCATC 
CGTGTTAGCT GGAAAAATGC CACTTCAGAA TGCAATGTGG AAAATGGTTT ATCAATCTTT 
GATCGAGAGC CATTGCTCAT TGGGATTTCA GTCAGCTCTT TAGAGAAAGC TTTTAGAGTG 
GTCGATGTTG GTCCAAATGC TGAGCATAAA GATGAGGCTC TTAAATTCCG GAAGTTTTGG 
GGAGAGAAAG CAGAGCTTCG AAGATTTAAA GATGGGATGA TTGCAGAAAG CACAGTATGG 
GAAAGCAAGC AATGGGAAAG GCATACCATC ATAAAAAGGA TTACCGAATA TCTCCTTTTG 
CGGCACCTTT CCTTGTCAGA AAGAAATATC GTGCATATTG TAGATCAACT TGATTTCTCT 
CTGGTTAATG GTGTTGGAGA TTCTATCTCA TTTTCTGGAA GTTTGCTTGA GGCATTTGAA 
GTGTTGTCAA AGCGATTACA TCTTCTCAAA GACATTCCTT TGAAGGTGTC TAGTGTGCAG 
CCTTTAGATT CAGCTTTCAG GTTTACATCT GTCTTCCCTC CTGAACCTCA TCCCCTAGCC 
AACGAAAAGA GTGCTGTTCC TAGATTAAAT AAGCTCACTT CAACCTGTAT TCAACCATTG 
GAAGTTATGA TTCAGTTGGA AGGTTCAGGC AACTGGCCAA TGGATGATGT AGCTATTGAG 
AAGACTAAAT CTGCTTTCCT TCTAAGAATT GGAGAAAGTT ATGTCAGTCT CCAGAACAAT 
TGGGGTATGA TCTGCACTGC TACTGAGGAA AATGTGGATG TTTTTATGTC TGGATATGCA 
TTTCGTCTTA GAATTTTGCA TGAAAGAGGC CTGAGTTTGT TGAATAGGCA AAATGGGAGT 
AATCAACTGA AGCATATTTC TTCTGTTGAC AAGGAACTTT TTACCCGTGG CCAGCATTCT 
AGCATGATTA ATGGTCTACA GGGTTGTTAC CCAATATATG GGCCAGTTGT TCGGCTAGCG 
AAGCGATGGG TTGCTTCGCA TCTCTTTTCA GCTTGCTTGG TCGAGGAGGC AGTTGAGCTA 
TTGGTTGCAT ATCTCTTTTT GAAGCCTTTA CCATTTTATG TGCCTTGCTC TCGGATATCA 
GGATTTCTAA GGTTCTTACG GTTATTATCA GAGTATGACT GGAATTTCTC TGCTTTGGTG 
GTTGACATAA ATAGTGATTT GAGCCCAAGT GATGAAAAAG AGATTAATGA GAATTTTACT 
TCAAGTAGAA AAGGTTATGA AGAAAATGCC CAAAATGTAA ACCCGGCAAT GTTCTTAGCA 
ACAGCTTATG ATAAGGCATC TGAAGCTTGG ACCAGATTCT CACCAAACTC ATCAGAGCTT 
AGAAGGTTGG TGGCCTATGC CCGAAGCAGT GCAAACCTAT TGACTAAACT TATTTTGGGG 
GGTCAAATTG ATTCTTACAA ATGGGAGTGT CTTTTCCGTA CTCCCTTGAA CAACTATGAT 
GCTGTAATTC TTCTCCACAG GGAGAAAATG CCTTATCCCC AACGTCTTCT CTTCCCATCT 
GAGATGAACC AAGGGAAGCA TGTTGCTCAG GGCAATGCTA GCAAGGCTTT TCATCCATTT 
TTGTTGCCTG AACACATGAA AGGAAACTCC CCAGATTTGA AAGATACGCT ACTGGTAGAC 
TTTGATCCAC TGAGGTGCTT CATTGGGGAT TTAGAGGAAG AGTTTCCAAA CGCTTTCAAG 
TTGTGGTACG ATTCTCTAGG AGGTGATGCC ATTGGCATGA TGTGGGAGAG ATCATCTTCA 
AAGAAACGGG GACGAAGTGA AGAGAATGAG GAGGAAAAAG ATCCAGTCAA TGTGCTAAAA 
GCTGTCGGTG AGGTCGGGAA AGGGTTTGTG AGGAGTATTT ATCTACTCAA GTCCCCAAGG 
CTCAGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010649142.1
CDS	79..3270
Translation	MDSDTIMEPMDLKVRELLKEVQLDYSSATTKLVDDTVSAIKQAIDTIPEDLKVTADFAPQFVRDIGADKVEFNFKKPKLFEIGGSYSIRCVAKPDVDIDLFVRLPKECFHEKDYLNHRYHAKRFLYLCIIKKYLNSSSFIRKVEWSTLQNEARKPVLVVYPAMELAEVPGLSVRIIPTATSLFSILKLNLKRNNVCSLKQDESTPQATPKYNSSILEDMFLEDNAEFVKRTFLGWKELGEALILLKVWARQRSSIYAYDCLNGFLISVIMSYLATDSGRNLINNSMKPMQIFRVTLDFIATSKLWNTGLYFKSQSLLNISKEELLERKQYLRLFPVVISESLAHFNLAFRITGGGFLELQDEAVLTLSCIGKCKDGGFEELFMTKIDYPAKYDYCMRLNLKGNSDVYALGFCLDEECWRSFEQKVHFLLCQGLSDRAKFIRVSWKNATSECNVENGLSIFDREPLLIGISVSSLEKAFRVVDVGPNAEHKDEALKFRKFWGEKAELRRFKDGMIAESTVWESKQWERHTIIKRITEYLLLRHLSLSERNIVHIVDQLDFSLVNGVGDSISFSGSLLEAFEVLSKRLHLLKDIPLKVSSVQPLDSAFRFTSVFPPEPHPLANEKSAVPRLNKLTSTCIQPLEVMIQLEGSGNWPMDDVAIEKTKSAFLLRIGESYVSLQNNWGMICTATEENVDVFMSGYAFRLRILHERGLSLLNRQNGSNQLKHISSVDKELFTRGQHSSMINGLQGCYPIYGPVVRLAKRWVASHLFSACLVEEAVELLVAYLFLKPLPFYVPCSRISGFLRFLRLLSEYDWNFSALVVDINSDLSPSDEKEINENFTSSRKGYEENAQNVNPAMFLATAYDKASEAWTRFSPNSSELRRLVAYARSSANLLTKLILGGQIDSYKWECLFRTPLNNYDAVILLHREKMPYPQRLLFPSEMNQGKHVAQGNASKAFHPFLLPEHMKGNSPDLKDTLLVDFDPLRCFIGDLEEEFPNAFKLWYDSLGGDAIGMMWERSSSKKRGRSEENEEEKDPVNVLKAVGEVGKGFVRSIYLLKSPRLRN

Target ORF information:

RefSeq Version	XM_010650840.2
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera nucleolar protein 6 (LOC100242719), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010650840.2

ORF Insert Sequence:

ATGGATTCTG ATACGATTAT GGAACCGATG GATTTGAAGG TGCGAGAGCT GTTGAAGGAG 
GTACAGCTCG ACTACTCTTC TGCAACAACA AAGCTCGTCG ACGACACCGT TTCAGCCATT 
AAACAAGCCA TCGATACAAT CCCTGAAGAC TTGAAGGTTA CAGCAGATTT TGCTCCACAA 
TTTGTCAGAG ACATTGGCGC TGATAAAGTT GAATTCAACT TCAAGAAGCC AAAATTATTC 
GAAATTGGTG GTAGTTATTC AATACGCTGT GTGGCAAAGC CTGATGTAGA TATTGATCTT 
TTTGTTAGGT TGCCAAAGGA GTGTTTCCAT GAGAAGGACT ATTTGAATCA CCGTTACCAT 
GCAAAAAGGT TCCTCTATCT CTGCATAATC AAGAAGTATT TGAACTCTTC TTCCTTCATT 
CGAAAGGTTG AATGGTCCAC CCTACAAAAT GAGGCTCGAA AACCAGTATT GGTTGTCTAT 
CCAGCTATGG AGCTTGCTGA AGTTCCTGGG CTTTCTGTGA GAATAATACC TACGGCAACA 
TCCCTGTTTA GTATTTTGAA GTTAAACTTG AAGCGGAACA ATGTTTGTTC TTTGAAGCAA 
GATGAGAGTA CTCCTCAGGC TACACCAAAA TACAATAGTA GTATTTTGGA AGACATGTTT 
CTAGAGGACA ATGCGGAATT TGTGAAGCGA ACTTTTCTTG GTTGGAAGGA GTTGGGGGAG 
GCTTTAATTT TGCTTAAGGT ATGGGCTCGG CAGAGAAGTT CGATATATGC TTATGATTGC 
TTAAATGGAT TTCTGATCTC TGTCATAATG TCATACCTTG CAACGGATTC TGGAAGGAAT 
CTCATTAACA ATTCAATGAA GCCAATGCAG ATATTTCGTG TGACCTTGGA TTTCATTGCC 
ACATCTAAGC TATGGAATAC TGGTCTTTAT TTCAAGTCTC AAAGCCTACT GAATATTTCA 
AAGGAGGAGT TGCTGGAAAG GAAGCAATAT TTAAGATTAT TTCCTGTAGT TATATCTGAG 
TCACTTGCTC ATTTTAATTT GGCGTTCCGG ATTACAGGAG GTGGCTTTTT GGAGCTCCAA 
GATGAGGCTG TTTTGACCCT TAGTTGCATA GGCAAATGTA AAGATGGTGG GTTTGAGGAG 
CTTTTTATGA CTAAGATTGA CTATCCAGCT AAATATGACT ACTGCATGAG ATTGAATTTG 
AAAGGAAATA GTGATGTTTA TGCATTGGGG TTTTGCTTGG ATGAGGAATG TTGGAGATCC 
TTTGAGCAGA AAGTTCATTT TCTTCTGTGT CAAGGATTGA GTGATAGAGC AAAGTTCATC 
CGTGTTAGCT GGAAAAATGC CACTTCAGAA TGCAATGTGG AAAATGGTTT ATCAATCTTT 
GATCGAGAGC CATTGCTCAT TGGGATTTCA GTCAGCTCTT TAGAGAAAGC TTTTAGAGTG 
GTCGATGTTG GTCCAAATGC TGAGCATAAA GATGAGGCTC TTAAATTCCG GAAGTTTTGG 
GGAGAGAAAG CAGAGCTTCG AAGATTTAAA GATGGGATGA TTGCAGAAAG CACAGTATGG 
GAAAGCAAGC AATGGGAAAG GCATACCATC ATAAAAAGGA TTACCGAATA TCTCCTTTTG 
CGGCACCTTT CCTTGTCAGA AAGAAATATC GTGCATATTG TAGATCAACT TGATTTCTCT 
CTGGTTAATG GTGTTGGAGA TTCTATCTCA TTTTCTGGAA GTTTGCTTGA GGCATTTGAA 
GTGTTGTCAA AGCGATTACA TCTTCTCAAA GACATTCCTT TGAAGGTGTC TAGTGTGCAG 
CCTTTAGATT CAGCTTTCAG GTTTACATCT GTCTTCCCTC CTGAACCTCA TCCCCTAGCC 
AACGAAAAGA GTGCTGTTCC TAGATTAAAT AAGCTCACTT CAACCTGTAT TCAACCATTG 
GAAGTTATGA TTCAGTTGGA AGGTTCAGGC AACTGGCCAA TGGATGATGT AGCTATTGAG 
AAGACTAAAT CTGCTTTCCT TCTAAGAATT GGAGAAAGTT ATGTCAGTCT CCAGAACAAT 
TGGGGTATGA TCTGCACTGC TACTGAGGAA AATGTGGATG TTTTTATGTC TGGATATGCA 
TTTCGTCTTA GAATTTTGCA TGAAAGAGGC CTGAGTTTGT TGAATAGGCA AAATGGGAGT 
AATCAACTGA AGCATATTTC TTCTGTTGAC AAGGAACTTT TTACCCGTGG CCAGCATTCT 
AGCATGATTA ATGGTCTACA GGGTTGTTAC CCAATATATG GGCCAGTTGT TCGGCTAGCG 
AAGCGATGGG TTGCTTCGCA TCTCTTTTCA GCTTGCTTGG TCGAGGAGGC AGTTGAGCTA 
TTGGTTGCAT ATCTCTTTTT GAAGCCTTTA CCATTTTATG TGCCTTGCTC TCGGATATCA 
GGATTTCTAA GGTTCTTACG GTTATTATCA GAGTATGACT GGAATTTCTC TGCTTTGGTG 
GTTGACATAA ATAGTGATTT GAGCCCAAGT GATGAAAAAG AGATTAATGA GAATTTTACT 
TCAAGTAGAA AAGGTTATGA AGAAAATGCC CAAAATGTAA ACCCGGCAAT GTTCTTAGCA 
ACAGCTTATG ATAAGGCATC TGAAGCTTGG ACCAGATTCT CACCAAACTC ATCAGAGCTT 
AGAAGGTTGG TGGCCTATGC CCGAAGCAGT GCAAACCTAT TGACTAAACT TATTTTGGGG 
GGTCAAATTG ATTCTTACAA ATGGGAGTGT CTTTTCCGTA CTCCCTTGAA CAACTATGAT 
GCTGTAATTC TTCTCCACAG GGAGAAAATG CCTTATCCCC AACGTCTTCT CTTCCCATCT 
GAGATGAACC AAGGGAAGCA TGTTGCTCAG GGCAATGCTA GCAAGGCTTT TCATCCATTT 
TTGTTGCCTG AACACATGAA AGGAAACTCC CCAGATTTGA AAGATACGCT ACTGGTAGAC 
TTTGATCCAC TGAGGTGCTT CATTGGGGAT TTAGAGGAAG AGTTTCCAAA CGCTTTCAAG 
TTGTGGTACG ATTCTCTAGG AGGTGATGCC ATTGGCATGA TGTGGGAGAG ATCATCTTCA 
AAGAAACGGG GACGAAGTGA AGAGAATGAG GAGGAAAAAG ATCCAGTCAA TGTGCTAAAA 
GCTGTCGGTG AGGTCGGGAA AGGGTTTGTG AGGAGTATTT ATCTACTCAA GTCCCCAAGG 
CTCAGGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100242719 ( XM_010650841.2 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_010649143.1 Vitis vinifera nucleolar protein 6 (LOC100242719), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OVa23801
Clone ID Related Accession (Same CDS sequence)	XM_010650841.2
Accession Version	XM_010650841.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479916800000
Organism	Vitis vinifera(wine grape)
Product	nucleolar protein 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012010.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 25, 2016 this sequence version replaced XM_010650841.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG ATACGATTAT GGAACCGATG GATTTGAAGG TGCGAGAGCT GTTGAAGGAG 
GTACAGCTCG ACTACTCTTC TGCAACAACA AAGCTCGTCG ACGACACCGT TTCAGCCATT 
AAACAAGCCA TCGATACAAT CCCTGAAGAC TTGAAGGTTA CAGCAGATTT TGCTCCACAA 
TTTGTCAGAG ACATTGGCGC TGATAAAGTT GAATTCAACT TCAAGAAGCC AAAATTATTC 
GAAATTGGTG GTAGTTATTC AATACGCTGT GTGGCAAAGC CTGATGTAGA TATTGATCTT 
TTTGTTAGGT TGCCAAAGGA GTGTTTCCAT GAGAAGGACT ATTTGAATCA CCGTTACCAT 
GCAAAAAGGT TCCTCTATCT CTGCATAATC AAGAAGTATT TGAACTCTTC TTCCTTCATT 
CGAAAGGTTG AATGGTCCAC CCTACAAAAT GAGGCTCGAA AACCAGTATT GGTTGTCTAT 
CCAGCTATGG AGCTTGCTGA AGTTCCTGGG CTTTCTGTGA GAATAATACC TACGGCAACA 
TCCCTGTTTA GTATTTTGAA GTTAAACTTG AAGCGGAACA ATGTTTGTTC TTTGAAGCAA 
GATGAGAGTA CTCCTCAGGC TACACCAAAA TACAATAGTA GTATTTTGGA AGACATGTTT 
CTAGAGGACA ATGCGGAATT TGTGAAGCGA ACTTTTCTTG GTTGGAAGGA GTTGGGGGAG 
GCTTTAATTT TGCTTAAGGT ATGGGCTCGG CAGAGAAGTT CGATATATGC TTATGATTGC 
TTAAATGGAT TTCTGATCTC TGTCATAATG TCATACCTTG CAACGGATTC TGGAAGGAAT 
CTCATTAACA ATTCAATGAA GCCAATGCAG ATATTTCGTG TGACCTTGGA TTTCATTGCC 
ACATCTAAGC TATGGAATAC TGGTCTTTAT TTCAAGTCTC AAAGCCTACT GAATATTTCA 
AAGGAGGAGT TGCTGGAAAG GAAGCAATAT TTAAGATTAT TTCCTGTAGT TATATCTGAG 
TCACTTGCTC ATTTTAATTT GGCGTTCCGG ATTACAGGAG GTGGCTTTTT GGAGCTCCAA 
GATGAGGCTG TTTTGACCCT TAGTTGCATA GGCAAATGTA AAGATGGTGG GTTTGAGGAG 
CTTTTTATGA CTAAGATTGA CTATCCAGCT AAATATGACT ACTGCATGAG ATTGAATTTG 
AAAGGAAATA GTGATGTTTA TGCATTGGGG TTTTGCTTGG ATGAGGAATG TTGGAGATCC 
TTTGAGCAGA AAGTTCATTT TCTTCTGTGT CAAGGATTGA GTGATAGAGC AAAGTTCATC 
CGTGTTAGCT GGAAAAATGC CACTTCAGAA TGCAATGTGG AAAATGGTTT ATCAATCTTT 
GATCGAGAGC CATTGCTCAT TGGGATTTCA GTCAGCTCTT TAGAGAAAGC TTTTAGAGTG 
GTCGATGTTG GTCCAAATGC TGAGCATAAA GATGAGGCTC TTAAATTCCG GAAGTTTTGG 
GGAGAGAAAG CAGAGCTTCG AAGATTTAAA GATGGGATGA TTGCAGAAAG CACAGTATGG 
GAAAGCAAGC AATGGGAAAG GCATACCATC ATAAAAAGGA TTACCGAATA TCTCCTTTTG 
CGGCACCTTT CCTTGTCAGA AAGAAATATC GTGCATATTG TAGATCAACT TGATTTCTCT 
CTGGTTAATG GTGTTGGAGA TTCTATCTCA TTTTCTGGAA GTTTGCTTGA GGCATTTGAA 
GTGTTGTCAA AGCGATTACA TCTTCTCAAA GACATTCCTT TGAAGGTGTC TAGTGTGCAG 
CCTTTAGATT CAGCTTTCAG GTTTACATCT GTCTTCCCTC CTGAACCTCA TCCCCTAGCC 
AACGAAAAGA GTGCTGTTCC TAGATTAAAT AAGCTCACTT CAACCTGTAT TCAACCATTG 
GAAGTTATGA TTCAGTTGGA AGGTTCAGGC AACTGGCCAA TGGATGATGT AGCTATTGAG 
AAGACTAAAT CTGCTTTCCT TCTAAGAATT GGAGAAAGTC TCCAGAACAA TTGGGGTATG 
ATCTGCACTG CTACTGAGGA AAATGTGGAT GTTTTTATGT CTGGATATGC ATTTCGTCTT 
AGAATTTTGC ATGAAAGAGG CCTGAGTTTG TTGAATAGGC AAAATGGGAG TAATCAACTG 
AAGCATATTT CTTCTGTTGA CAAGGAACTT TTTACCCGTG GCCAGCATTC TAGCATGATT 
AATGGTCTAC AGGGTTGTTA CCCAATATAT GGGCCAGTTG TTCGGCTAGC GAAGCGATGG 
GTTGCTTCGC ATCTCTTTTC AGCTTGCTTG GTCGAGGAGG CAGTTGAGCT ATTGGTTGCA 
TATCTCTTTT TGAAGCCTTT ACCATTTTAT GTGCCTTGCT CTCGGATATC AGGATTTCTA 
AGGTTCTTAC GGTTATTATC AGAGTATGAC TGGAATTTCT CTGCTTTGGT GGTTGACATA 
AATAGTGATT TGAGCCCAAG TGATGAAAAA GAGATTAATG AGAATTTTAC TTCAAGTAGA 
AAAGGTTATG AAGAAAATGC CCAAAATGTA AACCCGGCAA TGTTCTTAGC AACAGCTTAT 
GATAAGGCAT CTGAAGCTTG GACCAGATTC TCACCAAACT CATCAGAGCT TAGAAGGTTG 
GTGGCCTATG CCCGAAGCAG TGCAAACCTA TTGACTAAAC TTATTTTGGG GGGTCAAATT 
GATTCTTACA AATGGGAGTG TCTTTTCCGT ACTCCCTTGA ACAACTATGA TGCTGTAATT 
CTTCTCCACA GGGAGAAAAT GCCTTATCCC CAACGTCTTC TCTTCCCATC TGAGATGAAC 
CAAGGGAAGC ATGTTGCTCA GGGCAATGCT AGCAAGGCTT TTCATCCATT TTTGTTGCCT 
GAACACATGA AAGGAAACTC CCCAGATTTG AAAGATACGC TACTGGTAGA CTTTGATCCA 
CTGAGGTGCT TCATTGGGGA TTTAGAGGAA GAGTTTCCAA ACGCTTTCAA GTTGTGGTAC 
GATTCTCTAG GAGGTGATGC CATTGGCATG ATGTGGGAGA GATCATCTTC AAAGAAACGG 
GGACGAAGTG AAGAGAATGA GGAGGAAAAA GATCCAGTCA ATGTGCTAAA AGCTGTCGGT 
GAGGTCGGGA AAGGGTTTGT GAGGAGTATT TATCTACTCA AGTCCCCAAG GCTCAGGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010649143.1
CDS	78..3260
Translation	MDSDTIMEPMDLKVRELLKEVQLDYSSATTKLVDDTVSAIKQAIDTIPEDLKVTADFAPQFVRDIGADKVEFNFKKPKLFEIGGSYSIRCVAKPDVDIDLFVRLPKECFHEKDYLNHRYHAKRFLYLCIIKKYLNSSSFIRKVEWSTLQNEARKPVLVVYPAMELAEVPGLSVRIIPTATSLFSILKLNLKRNNVCSLKQDESTPQATPKYNSSILEDMFLEDNAEFVKRTFLGWKELGEALILLKVWARQRSSIYAYDCLNGFLISVIMSYLATDSGRNLINNSMKPMQIFRVTLDFIATSKLWNTGLYFKSQSLLNISKEELLERKQYLRLFPVVISESLAHFNLAFRITGGGFLELQDEAVLTLSCIGKCKDGGFEELFMTKIDYPAKYDYCMRLNLKGNSDVYALGFCLDEECWRSFEQKVHFLLCQGLSDRAKFIRVSWKNATSECNVENGLSIFDREPLLIGISVSSLEKAFRVVDVGPNAEHKDEALKFRKFWGEKAELRRFKDGMIAESTVWESKQWERHTIIKRITEYLLLRHLSLSERNIVHIVDQLDFSLVNGVGDSISFSGSLLEAFEVLSKRLHLLKDIPLKVSSVQPLDSAFRFTSVFPPEPHPLANEKSAVPRLNKLTSTCIQPLEVMIQLEGSGNWPMDDVAIEKTKSAFLLRIGESLQNNWGMICTATEENVDVFMSGYAFRLRILHERGLSLLNRQNGSNQLKHISSVDKELFTRGQHSSMINGLQGCYPIYGPVVRLAKRWVASHLFSACLVEEAVELLVAYLFLKPLPFYVPCSRISGFLRFLRLLSEYDWNFSALVVDINSDLSPSDEKEINENFTSSRKGYEENAQNVNPAMFLATAYDKASEAWTRFSPNSSELRRLVAYARSSANLLTKLILGGQIDSYKWECLFRTPLNNYDAVILLHREKMPYPQRLLFPSEMNQGKHVAQGNASKAFHPFLLPEHMKGNSPDLKDTLLVDFDPLRCFIGDLEEEFPNAFKLWYDSLGGDAIGMMWERSSSKKRGRSEENEEEKDPVNVLKAVGEVGKGFVRSIYLLKSPRLRN

Target ORF information:

RefSeq Version	XM_010650841.2
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera nucleolar protein 6 (LOC100242719), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010650841.2

ORF Insert Sequence:

ATGGATTCTG ATACGATTAT GGAACCGATG GATTTGAAGG TGCGAGAGCT GTTGAAGGAG 
GTACAGCTCG ACTACTCTTC TGCAACAACA AAGCTCGTCG ACGACACCGT TTCAGCCATT 
AAACAAGCCA TCGATACAAT CCCTGAAGAC TTGAAGGTTA CAGCAGATTT TGCTCCACAA 
TTTGTCAGAG ACATTGGCGC TGATAAAGTT GAATTCAACT TCAAGAAGCC AAAATTATTC 
GAAATTGGTG GTAGTTATTC AATACGCTGT GTGGCAAAGC CTGATGTAGA TATTGATCTT 
TTTGTTAGGT TGCCAAAGGA GTGTTTCCAT GAGAAGGACT ATTTGAATCA CCGTTACCAT 
GCAAAAAGGT TCCTCTATCT CTGCATAATC AAGAAGTATT TGAACTCTTC TTCCTTCATT 
CGAAAGGTTG AATGGTCCAC CCTACAAAAT GAGGCTCGAA AACCAGTATT GGTTGTCTAT 
CCAGCTATGG AGCTTGCTGA AGTTCCTGGG CTTTCTGTGA GAATAATACC TACGGCAACA 
TCCCTGTTTA GTATTTTGAA GTTAAACTTG AAGCGGAACA ATGTTTGTTC TTTGAAGCAA 
GATGAGAGTA CTCCTCAGGC TACACCAAAA TACAATAGTA GTATTTTGGA AGACATGTTT 
CTAGAGGACA ATGCGGAATT TGTGAAGCGA ACTTTTCTTG GTTGGAAGGA GTTGGGGGAG 
GCTTTAATTT TGCTTAAGGT ATGGGCTCGG CAGAGAAGTT CGATATATGC TTATGATTGC 
TTAAATGGAT TTCTGATCTC TGTCATAATG TCATACCTTG CAACGGATTC TGGAAGGAAT 
CTCATTAACA ATTCAATGAA GCCAATGCAG ATATTTCGTG TGACCTTGGA TTTCATTGCC 
ACATCTAAGC TATGGAATAC TGGTCTTTAT TTCAAGTCTC AAAGCCTACT GAATATTTCA 
AAGGAGGAGT TGCTGGAAAG GAAGCAATAT TTAAGATTAT TTCCTGTAGT TATATCTGAG 
TCACTTGCTC ATTTTAATTT GGCGTTCCGG ATTACAGGAG GTGGCTTTTT GGAGCTCCAA 
GATGAGGCTG TTTTGACCCT TAGTTGCATA GGCAAATGTA AAGATGGTGG GTTTGAGGAG 
CTTTTTATGA CTAAGATTGA CTATCCAGCT AAATATGACT ACTGCATGAG ATTGAATTTG 
AAAGGAAATA GTGATGTTTA TGCATTGGGG TTTTGCTTGG ATGAGGAATG TTGGAGATCC 
TTTGAGCAGA AAGTTCATTT TCTTCTGTGT CAAGGATTGA GTGATAGAGC AAAGTTCATC 
CGTGTTAGCT GGAAAAATGC CACTTCAGAA TGCAATGTGG AAAATGGTTT ATCAATCTTT 
GATCGAGAGC CATTGCTCAT TGGGATTTCA GTCAGCTCTT TAGAGAAAGC TTTTAGAGTG 
GTCGATGTTG GTCCAAATGC TGAGCATAAA GATGAGGCTC TTAAATTCCG GAAGTTTTGG 
GGAGAGAAAG CAGAGCTTCG AAGATTTAAA GATGGGATGA TTGCAGAAAG CACAGTATGG 
GAAAGCAAGC AATGGGAAAG GCATACCATC ATAAAAAGGA TTACCGAATA TCTCCTTTTG 
CGGCACCTTT CCTTGTCAGA AAGAAATATC GTGCATATTG TAGATCAACT TGATTTCTCT 
CTGGTTAATG GTGTTGGAGA TTCTATCTCA TTTTCTGGAA GTTTGCTTGA GGCATTTGAA 
GTGTTGTCAA AGCGATTACA TCTTCTCAAA GACATTCCTT TGAAGGTGTC TAGTGTGCAG 
CCTTTAGATT CAGCTTTCAG GTTTACATCT GTCTTCCCTC CTGAACCTCA TCCCCTAGCC 
AACGAAAAGA GTGCTGTTCC TAGATTAAAT AAGCTCACTT CAACCTGTAT TCAACCATTG 
GAAGTTATGA TTCAGTTGGA AGGTTCAGGC AACTGGCCAA TGGATGATGT AGCTATTGAG 
AAGACTAAAT CTGCTTTCCT TCTAAGAATT GGAGAAAGTC TCCAGAACAA TTGGGGTATG 
ATCTGCACTG CTACTGAGGA AAATGTGGAT GTTTTTATGT CTGGATATGC ATTTCGTCTT 
AGAATTTTGC ATGAAAGAGG CCTGAGTTTG TTGAATAGGC AAAATGGGAG TAATCAACTG 
AAGCATATTT CTTCTGTTGA CAAGGAACTT TTTACCCGTG GCCAGCATTC TAGCATGATT 
AATGGTCTAC AGGGTTGTTA CCCAATATAT GGGCCAGTTG TTCGGCTAGC GAAGCGATGG 
GTTGCTTCGC ATCTCTTTTC AGCTTGCTTG GTCGAGGAGG CAGTTGAGCT ATTGGTTGCA 
TATCTCTTTT TGAAGCCTTT ACCATTTTAT GTGCCTTGCT CTCGGATATC AGGATTTCTA 
AGGTTCTTAC GGTTATTATC AGAGTATGAC TGGAATTTCT CTGCTTTGGT GGTTGACATA 
AATAGTGATT TGAGCCCAAG TGATGAAAAA GAGATTAATG AGAATTTTAC TTCAAGTAGA 
AAAGGTTATG AAGAAAATGC CCAAAATGTA AACCCGGCAA TGTTCTTAGC AACAGCTTAT 
GATAAGGCAT CTGAAGCTTG GACCAGATTC TCACCAAACT CATCAGAGCT TAGAAGGTTG 
GTGGCCTATG CCCGAAGCAG TGCAAACCTA TTGACTAAAC TTATTTTGGG GGGTCAAATT 
GATTCTTACA AATGGGAGTG TCTTTTCCGT ACTCCCTTGA ACAACTATGA TGCTGTAATT 
CTTCTCCACA GGGAGAAAAT GCCTTATCCC CAACGTCTTC TCTTCCCATC TGAGATGAAC 
CAAGGGAAGC ATGTTGCTCA GGGCAATGCT AGCAAGGCTT TTCATCCATT TTTGTTGCCT 
GAACACATGA AAGGAAACTC CCCAGATTTG AAAGATACGC TACTGGTAGA CTTTGATCCA 
CTGAGGTGCT TCATTGGGGA TTTAGAGGAA GAGTTTCCAA ACGCTTTCAA GTTGTGGTAC 
GATTCTCTAG GAGGTGATGC CATTGGCATG ATGTGGGAGA GATCATCTTC AAAGAAACGG 
GGACGAAGTG AAGAGAATGA GGAGGAAAAA GATCCAGTCA ATGTGCTAAA AGCTGTCGGT 
GAGGTCGGGA AAGGGTTTGT GAGGAGTATT TATCTACTCA AGTCCCCAAG GCTCAGGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100242719 cDNA ORF clone, Vitis vinifera(wine grape)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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