PIP2;3 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIP2;3
Entrez Gene ID	100233094
Full Name	aquaporin-like
Synonyms	PIP2
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281129.1	NP_001268058.1	aquaporin-like

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Exploring three PIPs and three TIPs of grapevine for transport of water and atypical substrates through heterologous expression in aqy-null yeast.
Sabir F, Leandro MJ, Martins AP, Loureiro-Dias MC, Moura TF, Soveral G, Prista C
PloS one9(8)e102087(2014)

A putative role for TIP and PIP aquaporins in dynamics of leaf hydraulic and stomatal conductances in grapevine under water stress and re-watering.
Pou A, Medrano H, Flexas J, Tyerman SD
Plant, cell & environment36(4)828-43(2013 Apr)

The role of plasma membrane intrinsic protein aquaporins in water transport through roots: diurnal and drought stress responses reveal different strategies between isohydric and anisohydric cultivars of grapevine.
Vandeleur RK, Mayo G, Shelden MC, Gilliham M, Kaiser BN, Tyerman SD
Plant physiology149(1)445-60(2009 Jan)

Identification of grapevine aquaporins and expression analysis in developing berries.
Fouquet R, Léon C, Ollat N, Barrieu F
Plant cell reports27(9)1541-50(2008 Sep)

A high quality draft consensus sequence of the genome of a heterozygous grapevine variety.
Velasco R, Zharkikh A, Troggio M, Cartwright DA, Cestaro A, Pruss D, Pindo M, Fitzgerald LM, Vezzulli S, Reid J, Malacarne G, Iliev D, Coppola G, Wardell B, Micheletti D, Macalma T, Facci M, Mitchell JT, Perazzolli M, Eldredge G, Gatto P, Oyzerski R, Moretto M, Gutin N, Stefanini M, Chen Y, Segala C, Davenport C, Demattè L, Mraz A, Battilana J, Stormo K, Costa F, Tao Q, Si-Ammour A, Harkins T, Lackey A, Perbost C, Taillon B, Stella A, Solovyev V, Fawcett JA, Sterck L, Vandepoele K, Grando SM, Toppo S, Moser C, Lanchbury J, Bogden R, Skolnick M, Sgaramella V, Bhatnagar SK, Fontana P, Gutin A, Van de Peer Y, Salamini F, Viola R
PloS one2(12)e1326(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PIP2;3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIP2;3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa22937	NM_001281129.1 Latest version!	Vitis vinifera aquaporin-like (PIP2;3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIP2;3 ( NM_001281129.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> NP_001268058.1 Vitis vinifera aquaporin-like (PIP2;3), mRNA.

Price & Availability↑

CloneID	OVa22937
Clone ID Related Accession (Same CDS sequence)	NM_001281129.1
Accession Version	NM_001281129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556899200000
Organism	Vitis vinifera(wine grape)
Product	aquaporin-like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF364437.1. On Jul 24, 2013 this sequence version replaced XM_002281196.2. ##Evidence-Data-START## Transcript exon combination :: EF364437.1, DQ834700.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3598469, SAMEA3598470 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAGG ACATTGAGGT TGCAGGGCAC GGAGACCTCA CATTGAAGGA CTACCACGAC 
CCACCACCGG CGCCACTGGT CGACCCTGAG GAATTGGGGT CCTGGTCCTT TTACAGGGCC 
ATCATTGCCG AGTTCGTGGC CACCCTTTTG TTCCTCTACG TTACTGTGTT GACGGTGATT 
GGGTACAAGA ACCAGACTGA TCCCTACCAT CACGGAAATG AATGCAACGG GGTTGGTATT 
CTTGGCATTG CTTGGGCCTT TGGTGGCATG ATCTTCATCC TTGTTTACTG CACCGCCGGC 
ATCTCTGGAG GGCATATTAA TCCTGCAGTG ACGTTCGGGC TATTCCTGGC TAGAAAGGTG 
TCGCTGGTCC GAGCCGTGAT GTACATGGTG GCCCAGTGCG GGGGAGCCAT CTGCGGCTGT 
GGCCTGGTCA AGCTATTCCA GGATATTTAC TACATCAGAT ACGGTGGTGG CGCCAATGAG 
CTCGCCTCTG GATACAGTAT AGGAACGGGG TTGGCTGCTG AGATTATTGG CACCTTCGTT 
CTTGTCTACA CCGTCTTCTC TGCTACTGAT CCCAAAAGAA ATGCAAGAGA TTCCCATGTA 
CCTGTTTTGG CACCACTCCC CATTGGATTT GCTGTGTTCA TGGTTCACTT AGCCACGATC 
CCCATCACCG GTACCGGAAT CAACCCGGCT CGAAGTCTCG GAGCTGCTGT CATCTACAAC 
CAACCCAAAG CCTGGAGTGA CCATTGGGTA TTTTGGGCTG GACCCTTCAT TGGTGCAGCC 
ATTGCAGCAT TCTATCACCA GTTCATATTG AGAGCTGGAG CTGTTAAAGC TCTAGGGTCT 
TTCAAGAGCA GTTCCCACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268058.1
CDS	79..942
Translation	MAKDIEVAGHGDLTLKDYHDPPPAPLVDPEELGSWSFYRAIIAEFVATLLFLYVTVLTVIGYKNQTDPYHHGNECNGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAVMYMVAQCGGAICGCGLVKLFQDIYYIRYGGGANELASGYSIGTGLAAEIIGTFVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYNQPKAWSDHWVFWAGPFIGAAIAAFYHQFILRAGAVKALGSFKSSSHM

Target ORF information:

RefSeq Version	NM_001281129.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera aquaporin-like (PIP2;3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281129.1

ORF Insert Sequence:

ATGGCCAAGG ACATTGAGGT TGCAGGGCAC GGAGACCTCA CATTGAAGGA CTACCACGAC 
CCACCACCGG CGCCACTGGT CGACCCTGAG GAATTGGGGT CCTGGTCCTT TTACAGGGCC 
ATCATTGCCG AGTTCGTGGC CACCCTTTTG TTCCTCTACG TTACTGTGTT GACGGTGATT 
GGGTACAAGA ACCAGACTGA TCCCTACCAT CACGGAAATG AATGCAACGG GGTTGGTATT 
CTTGGCATTG CTTGGGCCTT TGGTGGCATG ATCTTCATCC TTGTTTACTG CACCGCCGGC 
ATCTCTGGAG GGCATATTAA TCCTGCAGTG ACGTTCGGGC TATTCCTGGC TAGAAAGGTG 
TCGCTGGTCC GAGCCGTGAT GTACATGGTG GCCCAGTGCG GGGGAGCCAT CTGCGGCTGT 
GGCCTGGTCA AGCTATTCCA GGATATTTAC TACATCAGAT ACGGTGGTGG CGCCAATGAG 
CTCGCCTCTG GATACAGTAT AGGAACGGGG TTGGCTGCTG AGATTATTGG CACCTTCGTT 
CTTGTCTACA CCGTCTTCTC TGCTACTGAT CCCAAAAGAA ATGCAAGAGA TTCCCATGTA 
CCTGTTTTGG CACCACTCCC CATTGGATTT GCTGTGTTCA TGGTTCACTT AGCCACGATC 
CCCATCACCG GTACCGGAAT CAACCCGGCT CGAAGTCTCG GAGCTGCTGT CATCTACAAC 
CAACCCAAAG CCTGGAGTGA CCATTGGGTA TTTTGGGCTG GACCCTTCAT TGGTGCAGCC 
ATTGCAGCAT TCTATCACCA GTTCATATTG AGAGCTGGAG CTGTTAAAGC TCTAGGGTCT 
TTCAAGAGCA GTTCCCACAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exploring three PIPs and three TIPs of grapevine for transport of water and atypical substrates through heterologous expression in aqy-null yeast.
PloS one9(8)e102087(2014)
Sabir F,Leandro MJ,Martins AP,Loureiro-Dias MC,Moura TF,Soveral G,Prista C

A putative role for TIP and PIP aquaporins in dynamics of leaf hydraulic and stomatal conductances in grapevine under water stress and re-watering.
Plant, cell & environment36(4)828-43(2013 Apr)
Pou A,Medrano H,Flexas J,Tyerman SD

The role of plasma membrane intrinsic protein aquaporins in water transport through roots: diurnal and drought stress responses reveal different strategies between isohydric and anisohydric cultivars of grapevine.
Plant physiology149(1)445-60(2009 Jan)
Vandeleur RK,Mayo G,Shelden MC,Gilliham M,Kaiser BN,Tyerman SD

Identification of grapevine aquaporins and expression analysis in developing berries.
Plant cell reports27(9)1541-50(2008 Sep)
Fouquet R,Léon C,Ollat N,Barrieu F

A high quality draft consensus sequence of the genome of a heterozygous grapevine variety.
PloS one2(12)e1326(2007 Dec)
Velasco R,Zharkikh A,Troggio M,Cartwright DA,Cestaro A,Pruss D,Pindo M,Fitzgerald LM,Vezzulli S,Reid J,Malacarne G,Iliev D,Coppola G,Wardell B,Micheletti D,Macalma T,Facci M,Mitchell JT,Perazzolli M,Eldredge G,Gatto P,Oyzerski R,Moretto M,Gutin N,Stefanini M,Chen Y,Segala C,Davenport C,Demattè L,Mraz A,Battilana J,Stormo K,Costa F,Tao Q,Si-Ammour A,Harkins T,Lackey A,Perbost C,Taillon B,Stella A,Solovyev V,Fawcett JA,Sterck L,Vandepoele K,Grando SM,Toppo S,Moser C,Lanchbury J,Bogden R,Skolnick M,Sgaramella V,Bhatnagar SK,Fontana P,Gutin A,Van de Peer Y,Salamini F,Viola R

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PIP2;3 cDNA ORF clone, Vitis vinifera(wine grape)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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