CHI cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHI
Entrez Gene ID	100233078
Full Name	chalcone isomerase
Synonyms	CHI1,CHI2
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281104.1	NP_001268033.1	chalcone--flavonone isomerase 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves.
Gutha LR, Casassa LF, Harbertson JF, Naidu RA
BMC plant biology10187(2010 Aug)

A high quality draft consensus sequence of the genome of a heterozygous grapevine variety.
Velasco R, Zharkikh A, Troggio M, Cartwright DA, Cestaro A, Pruss D, Pindo M, Fitzgerald LM, Vezzulli S, Reid J, Malacarne G, Iliev D, Coppola G, Wardell B, Micheletti D, Macalma T, Facci M, Mitchell JT, Perazzolli M, Eldredge G, Gatto P, Oyzerski R, Moretto M, Gutin N, Stefanini M, Chen Y, Segala C, Davenport C, Demattè L, Mraz A, Battilana J, Stormo K, Costa F, Tao Q, Si-Ammour A, Harkins T, Lackey A, Perbost C, Taillon B, Stella A, Solovyev V, Fawcett JA, Sterck L, Vandepoele K, Grando SM, Toppo S, Moser C, Lanchbury J, Bogden R, Skolnick M, Sgaramella V, Bhatnagar SK, Fontana P, Gutin A, Van de Peer Y, Salamini F, Viola R
PloS one2(12)e1326(2007 Dec)

Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.).
Sparvoli F, Martin C, Scienza A, Gavazzi G, Tonelli C
Plant molecular biology24(5)743-55(1994 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CHI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa00006	NM_001281104.1 Latest version!	Vitis vinifera chalcone isomerase (CHI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHI ( NM_001281104.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> NP_001268033.1 Vitis vinifera chalcone isomerase (CHI), mRNA.

Price & Availability↑

CloneID	OVa00006
Clone ID Related Accession (Same CDS sequence)	NM_001281104.1
Accession Version	NM_001281104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568908800000
Organism	Vitis vinifera(wine grape)
Product	chalcone--flavonone isomerase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X75963.1. On Jul 24, 2013 this sequence version replaced XM_002282072.2. ##Evidence-Data-START## Transcript exon combination :: X75963.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02152320, SAMN02152321 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCAAG TGCCGTCAGT CACCGCAGTT CAGGTCGAGA ACGTCCTATT TCCGCCGTCG 
GTGAAGCCGC CGGGCTCCAC CAACGACCTC TTCCTCGGCG GTGCAGGGGT GAGAGGTTTG 
GAGATCCAAG GGAAGTTCGT GAAGTTCACA GCCATCGGAG TGTACTTGGA GAACAGCGCC 
GTTCCTACGC TCGCCGTCAA GTGGAAGGGC AAGACTGTGG AGGAGTTAGC GGATTCGGTT 
GACTTTTTCC GAGATGTCGT CACAGGCCCC TTTGAGAAAT TCACAAAGGT AACAACGATT 
TTGCCTTTGA CGGGTCGCCA GTATTCAGAC AAGGTGTCAG AGAATTGTGT TGCCTTTTGG 
AAATCTGTTG GAATTTACAC TGACGCAGAA GCCAAAGCCA TTGAGAAATT CAATGAGGTC 
TTGAAGGATG AAACCTTCCC ACCAGGCAAC TCCATTCTTT TCACTCATTC TCCCCTTGGA 
GCTTTAACGA TGAGCTTCTC CAAAGATGGG TCCCTACCAG AAGTTGGGAA TGCAGTGATA 
GAGAATAAAT TATTGACGGA GGCGGTACTG GAGTCCATCA TCGGCAAGCA TGGTGTTTCC 
CCTGAAGCGA AGAAGAGTTT GGCAGCAAGA TTATCGGAAT TATTCTGCAA GGAGGCTGGG 
GATGAGAAAA TTGAAGCTGA GAAGGTTGCT CCGGTGGCTT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268033.1
CDS	12..716
Translation	MSQVPSVTAVQVENVLFPPSVKPPGSTNDLFLGGAGVRGLEIQGKFVKFTAIGVYLENSAVPTLAVKWKGKTVEELADSVDFFRDVVTGPFEKFTKVTTILPLTGRQYSDKVSENCVAFWKSVGIYTDAEAKAIEKFNEVLKDETFPPGNSILFTHSPLGALTMSFSKDGSLPEVGNAVIENKLLTEAVLESIIGKHGVSPEAKKSLAARLSELFCKEAGDEKIEAEKVAPVAC

Target ORF information:

RefSeq Version	NM_001281104.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera chalcone isomerase (CHI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281104.1

ORF Insert Sequence:

ATGTCTCAAG TGCCGTCAGT CACCGCAGTT CAGGTCGAGA ACGTCCTATT TCCGCCGTCG 
GTGAAGCCGC CGGGCTCCAC CAACGACCTC TTCCTCGGCG GTGCAGGGGT GAGAGGTTTG 
GAGATCCAAG GGAAGTTCGT GAAGTTCACA GCCATCGGAG TGTACTTGGA GAACAGCGCC 
GTTCCTACGC TCGCCGTCAA GTGGAAGGGC AAGACTGTGG AGGAGTTAGC GGATTCGGTT 
GACTTTTTCC GAGATGTCGT CACAGGCCCC TTTGAGAAAT TCACAAAGGT AACAACGATT 
TTGCCTTTGA CGGGTCGCCA GTATTCAGAC AAGGTGTCAG AGAATTGTGT TGCCTTTTGG 
AAATCTGTTG GAATTTACAC TGACGCAGAA GCCAAAGCCA TTGAGAAATT CAATGAGGTC 
TTGAAGGATG AAACCTTCCC ACCAGGCAAC TCCATTCTTT TCACTCATTC TCCCCTTGGA 
GCTTTAACGA TGAGCTTCTC CAAAGATGGG TCCCTACCAG AAGTTGGGAA TGCAGTGATA 
GAGAATAAAT TATTGACGGA GGCGGTACTG GAGTCCATCA TCGGCAAGCA TGGTGTTTCC 
CCTGAAGCGA AGAAGAGTTT GGCAGCAAGA TTATCGGAAT TATTCTGCAA GGAGGCTGGG 
GATGAGAAAA TTGAAGCTGA GAAGGTTGCT CCGGTGGCTT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modulation of flavonoid biosynthetic pathway genes and anthocyanins due to virus infection in grapevine (Vitis vinifera L.) leaves.
BMC plant biology10187(2010 Aug)
Gutha LR,Casassa LF,Harbertson JF,Naidu RA

A high quality draft consensus sequence of the genome of a heterozygous grapevine variety.
PloS one2(12)e1326(2007 Dec)
Velasco R,Zharkikh A,Troggio M,Cartwright DA,Cestaro A,Pruss D,Pindo M,Fitzgerald LM,Vezzulli S,Reid J,Malacarne G,Iliev D,Coppola G,Wardell B,Micheletti D,Macalma T,Facci M,Mitchell JT,Perazzolli M,Eldredge G,Gatto P,Oyzerski R,Moretto M,Gutin N,Stefanini M,Chen Y,Segala C,Davenport C,Demattè L,Mraz A,Battilana J,Stormo K,Costa F,Tao Q,Si-Ammour A,Harkins T,Lackey A,Perbost C,Taillon B,Stella A,Solovyev V,Fawcett JA,Sterck L,Vandepoele K,Grando SM,Toppo S,Moser C,Lanchbury J,Bogden R,Skolnick M,Sgaramella V,Bhatnagar SK,Fontana P,Gutin A,Van de Peer Y,Salamini F,Viola R

Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.).
Plant molecular biology24(5)743-55(1994 Mar)
Sparvoli F,Martin C,Scienza A,Gavazzi G,Tonelli C

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CHI cDNA ORF clone, Vitis vinifera(wine grape)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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