PLD cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLD
Entrez Gene ID	100232987
Full Name	phospholipase D alpha
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001280968.1	NP_001267897.1	phospholipase D alpha

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The high polyphenol content of grapevine cultivar tannat berries is conferred primarily by genes that are not shared with the reference genome.
Da Silva C, Zamperin G, Ferrarini A, Minio A, Dal Molin A, Venturini L, Buson G, Tononi P, Avanzato C, Zago E, Boido E, Dellacassa E, Gaggero C, Pezzotti M, Carrau F, Delledonne M
The Plant cell25(12)4777-88(2013 Dec)

Cloning of phospholipase D from grape berry and its expression under heat acclimation.
Wan SB, Wang W, Wen PF, Chen JY, Kong WF, Pan QH, Zhan JC, Tian L, Liu HT, Huang WD
Journal of biochemistry and molecular biology40(4)595-603(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa22775	NM_001280968.1 Latest version!	Vitis vinifera phospholipase D alpha (PLD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLD ( NM_001280968.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> NP_001267897.1 Vitis vinifera phospholipase D alpha (PLD), mRNA.

Price & Availability↑

CloneID	OVa22775
Clone ID Related Accession (Same CDS sequence)	NM_001280968.1
Accession Version	NM_001280968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1481040000000
Organism	Vitis vinifera(wine grape)
Product	phospholipase D alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ333882.1. On Jul 24, 2013 this sequence version replaced XM_002268159.1. ##Evidence-Data-START## Transcript exon combination :: DQ333882.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3598469, SAMEA3598470 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGA TTCTGTTGCA CGGGACTCTA CATGCTACGA TATTTGGGAT CGACAGGCTC 
CACAGCGGAG GCGCTCCCAA ATTCTTCCGA AAGCTTGTGG AGAACATTGA GGAGACTGTC 
GGTTTTGGCA AAGGAACCTC TAAACTCTAT GCAACTATTG ATATAGGAAG AGCTAGAGTT 
GGGAGGACTC GGATGATAGA AAATGAACCC TCCAATCCCA GGTGGTATGA ATCATTTCAC 
ATTTACTGTG CCCATATGGC CGGGCATATT ATATTCACTG TAAAAGATGA CAATCCCATT 
GGGGCGACTT TGATTGGAAG AGCATCTGTA CCTATTCAGG AGATCTTAGG GGGAGAGGAA 
GTAGATAAAT GGGTAGAAAT TGTTAATGAA GAGCTGAAGC CTATACATGG AGGTTCTAAG 
ATCCATGTGA AACTACAATA TTTTGAAGTC ACTGCAGATC GTAGTTGGGG TCGGGGAATT 
AGAAGTCTTA AATTTCCTGG GGTTCCATTC ACATTCTTTT CACAGAGAAA AGGATGTCAC 
GTCTCTTTGT ACCAAGATGC TCATGTTCCA GATAATTTTG TCCCAAAAAT ACCCCTCGCC 
GATGGCAAGT ACTATGAGCC CCGCAGATGT TGGGAAGATG TCTTTGATGC AATCAATAAC 
GCAAAACACT TGATCTACAT TACTGGCTGG TCTGTATACA CAGAAATCAC CTTGGTGCGG 
GACTCAAGGA GACCAAAACC AGGAGGAGAC TTGACCATTG GTGAGTTGCT TAAGAAGAAG 
GCAAGTGAAG GTGTTAGGGT TCTAATGCTT GTTTGGGATG ATCGAACCTC TGTTCCTTTA 
CTGAAAAAGG ATGGCTTGAT GGGTACCCAT GACGAAGAAA CTGAACATTA CTTTCACGAC 
ACTGATGTAC ACTGTGTATT GTGCCCCCGC AATCCTGATG ATGGTGGAAG TATAGTTCAG 
GATTTACAAA TTTCTACCAT GTTCACTCAT CACCAGAAGA TTGTTGTGGT GGACAGCGAG 
ATGCCCAGTG GAGGATCAGA AAAGAGGAGG ATTGTAAGTT TTGTTGGGGG TATCGATCTC 
TGTGATGGTA GATATGATAC CCAATTCCAT TCACTTTTCA GGACACTAGA CACAGCACAC 
CATGACGATT TCCATCAGCC AAACTTTGAA GGTGCTTCAA TCCAAAAGGG TGGACCAAGG 
GAGCCTTGGC ATGATATCCA TTCCAGACTT GAAGGACCTA TTGCCTGGGA TGTTCTCTTT 
AATTTTGAGC AGAGATGGAG AAAGCAAGGT GGGAAGGATA TACTTCTTCA ATTGAGAGAT 
CTTGATGATG TTATTATTCC CCCATCCCCT GTTATGTTCC CTGATGACCA GGAGGTATGG 
AACGTCCAGT TATTCAGATC TATTGATGGT GGGGCTGCCT TTGGCTTCCC TGAGACACCT 
GAAGAGGCAG CAAGAGCTGG GCTTGTCAGT GGGAAGGACA ATATCATTGA CCGGAGCATT 
CAGGATGCTT ATATTAATGC TATTCGACGG GCAAAGGATT TCATTTATAT TGAAAATCAG 
TATTTTCTGG GAAGCTCTTT TGGCTGGGCT CCTGATGAGG GCATTAAGAT TGAAGACGTT 
GGTGCTTTGC ATCTGATTCC AAAGGAACTC CCACTGAAGA TTGCAAGTAA GATTGAGGCA 
GGGGAGAAGT TCACTGTTTA TATTGTAGTT CCAATGTGGC CAGAGGGTAT GCCAGAGAGT 
GGATCAGTCC AGGCAATATT AGATTGGCAG AGGAGGACAA TGGAGATGAT GTATAAAGAT 
ATCATTAAGG CTCTTGCTGA TAAGGGTATT GAGGATGACC CTAGAAATTA TTTAACATTT 
TTCTGCCTCG GAAATCGGGA GGTCAAGAAG AGTGGAGAAT ATGAACCTTC AGAGCATCCT 
GAGCCTGATA CAGACTACAG TAGAGCCCAG GAGGCCCGAC GTTTCATGAT TTATGTTCAT 
GCCAAGATGA TGATTGTTGA TGACGAATAC ATCATAATTG GATCCGCCAA CATAAACCAG 
AGATCCATGG ATGGTGCCAG AGACTCCGAG ATAGCCATGG GAGGATACCA ACCATATCAC 
TTGGCAAGCA GGCAGCCAGC ACGAGGTCAG ATCCATGGTT TCCGAATGTC ATTGTGGTAC 
GAGCACCTTG GCATGCTCGA TGAGTCCTTC CTCCAGCCAG AAAGCAAGGA GTGCATCCAA 
AAGGTGAACC AGATCGCTGA AAAATACTGG GATCTGTACG CAAGCGAGAC ACTTGAGCAT 
GACCTCCCTG GTCATCTCCT CCGCTACCCT ATAGCGGTCT CCTGTGGGGG AGACATAACA 
GAGCTTCCAG GAGCTGAGTT TTTCCCCGAC ACCAAGGCCC GCGTTCTTGG CACCAAGTCC 
GAATATTTGC CTCCAATCCT CACAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001267897.1
CDS	1..2430
Translation	MAQILLHGTLHATIFGIDRLHSGGAPKFFRKLVENIEETVGFGKGTSKLYATIDIGRARVGRTRMIENEPSNPRWYESFHIYCAHMAGHIIFTVKDDNPIGATLIGRASVPIQEILGGEEVDKWVEIVNEELKPIHGGSKIHVKLQYFEVTADRSWGRGIRSLKFPGVPFTFFSQRKGCHVSLYQDAHVPDNFVPKIPLADGKYYEPRRCWEDVFDAINNAKHLIYITGWSVYTEITLVRDSRRPKPGGDLTIGELLKKKASEGVRVLMLVWDDRTSVPLLKKDGLMGTHDEETEHYFHDTDVHCVLCPRNPDDGGSIVQDLQISTMFTHHQKIVVVDSEMPSGGSEKRRIVSFVGGIDLCDGRYDTQFHSLFRTLDTAHHDDFHQPNFEGASIQKGGPREPWHDIHSRLEGPIAWDVLFNFEQRWRKQGGKDILLQLRDLDDVIIPPSPVMFPDDQEVWNVQLFRSIDGGAAFGFPETPEEAARAGLVSGKDNIIDRSIQDAYINAIRRAKDFIYIENQYFLGSSFGWAPDEGIKIEDVGALHLIPKELPLKIASKIEAGEKFTVYIVVPMWPEGMPESGSVQAILDWQRRTMEMMYKDIIKALADKGIEDDPRNYLTFFCLGNREVKKSGEYEPSEHPEPDTDYSRAQEARRFMIYVHAKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLASRQPARGQIHGFRMSLWYEHLGMLDESFLQPESKECIQKVNQIAEKYWDLYASETLEHDLPGHLLRYPIAVSCGGDITELPGAEFFPDTKARVLGTKSEYLPPILTT

Target ORF information:

RefSeq Version	NM_001280968.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera phospholipase D alpha (PLD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001280968.1

ORF Insert Sequence:

ATGGCTCAGA TTCTGTTGCA CGGGACTCTA CATGCTACGA TATTTGGGAT CGACAGGCTC 
CACAGCGGAG GCGCTCCCAA ATTCTTCCGA AAGCTTGTGG AGAACATTGA GGAGACTGTC 
GGTTTTGGCA AAGGAACCTC TAAACTCTAT GCAACTATTG ATATAGGAAG AGCTAGAGTT 
GGGAGGACTC GGATGATAGA AAATGAACCC TCCAATCCCA GGTGGTATGA ATCATTTCAC 
ATTTACTGTG CCCATATGGC CGGGCATATT ATATTCACTG TAAAAGATGA CAATCCCATT 
GGGGCGACTT TGATTGGAAG AGCATCTGTA CCTATTCAGG AGATCTTAGG GGGAGAGGAA 
GTAGATAAAT GGGTAGAAAT TGTTAATGAA GAGCTGAAGC CTATACATGG AGGTTCTAAG 
ATCCATGTGA AACTACAATA TTTTGAAGTC ACTGCAGATC GTAGTTGGGG TCGGGGAATT 
AGAAGTCTTA AATTTCCTGG GGTTCCATTC ACATTCTTTT CACAGAGAAA AGGATGTCAC 
GTCTCTTTGT ACCAAGATGC TCATGTTCCA GATAATTTTG TCCCAAAAAT ACCCCTCGCC 
GATGGCAAGT ACTATGAGCC CCGCAGATGT TGGGAAGATG TCTTTGATGC AATCAATAAC 
GCAAAACACT TGATCTACAT TACTGGCTGG TCTGTATACA CAGAAATCAC CTTGGTGCGG 
GACTCAAGGA GACCAAAACC AGGAGGAGAC TTGACCATTG GTGAGTTGCT TAAGAAGAAG 
GCAAGTGAAG GTGTTAGGGT TCTAATGCTT GTTTGGGATG ATCGAACCTC TGTTCCTTTA 
CTGAAAAAGG ATGGCTTGAT GGGTACCCAT GACGAAGAAA CTGAACATTA CTTTCACGAC 
ACTGATGTAC ACTGTGTATT GTGCCCCCGC AATCCTGATG ATGGTGGAAG TATAGTTCAG 
GATTTACAAA TTTCTACCAT GTTCACTCAT CACCAGAAGA TTGTTGTGGT GGACAGCGAG 
ATGCCCAGTG GAGGATCAGA AAAGAGGAGG ATTGTAAGTT TTGTTGGGGG TATCGATCTC 
TGTGATGGTA GATATGATAC CCAATTCCAT TCACTTTTCA GGACACTAGA CACAGCACAC 
CATGACGATT TCCATCAGCC AAACTTTGAA GGTGCTTCAA TCCAAAAGGG TGGACCAAGG 
GAGCCTTGGC ATGATATCCA TTCCAGACTT GAAGGACCTA TTGCCTGGGA TGTTCTCTTT 
AATTTTGAGC AGAGATGGAG AAAGCAAGGT GGGAAGGATA TACTTCTTCA ATTGAGAGAT 
CTTGATGATG TTATTATTCC CCCATCCCCT GTTATGTTCC CTGATGACCA GGAGGTATGG 
AACGTCCAGT TATTCAGATC TATTGATGGT GGGGCTGCCT TTGGCTTCCC TGAGACACCT 
GAAGAGGCAG CAAGAGCTGG GCTTGTCAGT GGGAAGGACA ATATCATTGA CCGGAGCATT 
CAGGATGCTT ATATTAATGC TATTCGACGG GCAAAGGATT TCATTTATAT TGAAAATCAG 
TATTTTCTGG GAAGCTCTTT TGGCTGGGCT CCTGATGAGG GCATTAAGAT TGAAGACGTT 
GGTGCTTTGC ATCTGATTCC AAAGGAACTC CCACTGAAGA TTGCAAGTAA GATTGAGGCA 
GGGGAGAAGT TCACTGTTTA TATTGTAGTT CCAATGTGGC CAGAGGGTAT GCCAGAGAGT 
GGATCAGTCC AGGCAATATT AGATTGGCAG AGGAGGACAA TGGAGATGAT GTATAAAGAT 
ATCATTAAGG CTCTTGCTGA TAAGGGTATT GAGGATGACC CTAGAAATTA TTTAACATTT 
TTCTGCCTCG GAAATCGGGA GGTCAAGAAG AGTGGAGAAT ATGAACCTTC AGAGCATCCT 
GAGCCTGATA CAGACTACAG TAGAGCCCAG GAGGCCCGAC GTTTCATGAT TTATGTTCAT 
GCCAAGATGA TGATTGTTGA TGACGAATAC ATCATAATTG GATCCGCCAA CATAAACCAG 
AGATCCATGG ATGGTGCCAG AGACTCCGAG ATAGCCATGG GAGGATACCA ACCATATCAC 
TTGGCAAGCA GGCAGCCAGC ACGAGGTCAG ATCCATGGTT TCCGAATGTC ATTGTGGTAC 
GAGCACCTTG GCATGCTCGA TGAGTCCTTC CTCCAGCCAG AAAGCAAGGA GTGCATCCAA 
AAGGTGAACC AGATCGCTGA AAAATACTGG GATCTGTACG CAAGCGAGAC ACTTGAGCAT 
GACCTCCCTG GTCATCTCCT CCGCTACCCT ATAGCGGTCT CCTGTGGGGG AGACATAACA 
GAGCTTCCAG GAGCTGAGTT TTTCCCCGAC ACCAAGGCCC GCGTTCTTGG CACCAAGTCC 
GAATATTTGC CTCCAATCCT CACAACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The high polyphenol content of grapevine cultivar tannat berries is conferred primarily by genes that are not shared with the reference genome.
The Plant cell25(12)4777-88(2013 Dec)
Da Silva C,Zamperin G,Ferrarini A,Minio A,Dal Molin A,Venturini L,Buson G,Tononi P,Avanzato C,Zago E,Boido E,Dellacassa E,Gaggero C,Pezzotti M,Carrau F,Delledonne M

Cloning of phospholipase D from grape berry and its expression under heat acclimation.
Journal of biochemistry and molecular biology40(4)595-603(2007 Jul)
Wan SB,Wang W,Wen PF,Chen JY,Kong WF,Pan QH,Zhan JC,Tian L,Liu HT,Huang WD

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PLD cDNA ORF clone, Vitis vinifera(wine grape)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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