Tc00.1047053506435.120 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053506435.120
Entrez Gene ID	3553819
Full Name	ATP-dependent RNA helicase
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_815913.1	XP_821006.1	ATP-dependent RNA helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053506435.120 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053506435.120 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb15192	XM_815913.1 Latest version!	Trypanosoma cruzi strain CL Brener ATP-dependent RNA helicase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053506435.120 ( XM_815913.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_821006.1 Trypanosoma cruzi strain CL Brener ATP-dependent RNA helicase partial mRNA.

Price & Availability↑

CloneID	OTb15192
Clone ID Related Accession (Same CDS sequence)	XM_815913.1
Accession Version	XM_815913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	ATP-dependent RNA helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849433). COMPLETENESS: incomplete on both ends.

ATGCGACGAC TTTTGTGTAA ATCTGGCCTT CTGGCCCCGG CCCGGGAAAT GCGGCATGAG 
CGTGATTTCC GTGGCCGTAA TCGCCAACGC CGACAGCGTG GATTTGGCGA GGGCCGTAGC 
TCTTCCAACC CACGGAACAA TGACGACATT GGGATTTTGC AGATAAAATT TCACACCATA 
CCGGCTCTGC AGGTGGACAT GCGAGCAGAG ACTCAGATCG AGTCGTTCTT GAGATCGCAG 
CTGCGGGAGG ATGGGTCTTC GATGTTACTG TCAACGAATG CGTCATCTTT ACCCATAGTT 
CGTGTGATAA ATGTTCCCAG GTCCACCTTG AAGCGTGCCG TGCTGGAGTT CCACGTAAGG 
GGAATACCGG TGCGGGCTTT AGGGCAATCA ACCCGCGCAA AAAGGGCTAG GGCGCTCTGC 
TTCATGCATG CAGCGAGGCT CATTGATCAC TTTTCAGTAG AAGGCACGGA CAGCAGTGCT 
GCGCCTCTGC CACTCGAGGA GGGCACGGAA TTAAATGCTC AAAAGAAATA TTTGAAGCGT 
GTGATGAGTA TTCGAAAAAC GCAGGCTTTT GGTCCGCCTC CACAGCCGGA AGAATACGAT 
TGTTGGGAAG ATTATGTGGA TGAAAGTAGG CGACACATTG AACGGCAAGA GCAAGAGCGA 
CGAAACGAGG CCTTTCAGCT GCTGCGTATC CCTCATACTG GGAACCCATT GGTTGACAGG 
GCGGCGGAAA TGACCGAGCA GGAACGATGC ACCGACCGAT TGGCTCTTCA AAAACTTAAC 
AATGAACTTC TTGGGGTCAG CAAGGGTGTG AATATTGTTC GCATCTCGCA TCGTGTCTTT 
GTCGCCACAC TCACATTGGA TAGTGTGACA AATTTAGTTG CTACTGGTGT AGCTGAGAGT 
TCACAGGAGG CGAAGCAGCG TGCCGCGATG CATGCGCTTA ACATTCTTAC GCTGGTGAAG 
GAGCAACGGC AAGCTTCTGC CGTGGATGGG AAGGCATCAG CTTTTGCAGG CCGTGGAAGC 
TGTGCCTCGC CTGACGCCGT GTCACTCAGC CTTACGCCAC GTTATTCCAA GATGCTGGAC 
TTTTTTTCAC TTTTTTTTGG TGTGACTCCG GCTCCTGTCT TCACAAGGGA GGGAAATTCC 
TATATTTGTC ACATGGATTT GGACGGTGTC AAGTGCACGG GACAAGGCAT TAACCGGTTT 
GAAGCGGAGC GCCATGCCAT TGAGAACGCA TTGGGAGAGA TGGAGTTGTA TGACGAGCGG 
TTGCAGGGCA TTAATAGCAT CATTGCACAG TATCCGAACA TCCAGCCACG GTGTGTTCCG 
ACTGCCAAAT TGCCGGAGGC GTTGAGGAAT GAAATTCACG CCTTTGTACA GCGCTGTCGC 
ACCGAACTTC ATTTTCCAGT TGATGGCAAT ACGGTGGGAA ACGAAGGCCA TGGTGGTACT 
CCTGAAAAAA TTTTAACTGA TGACGTTTTG GATGCCGAGA CACGGGCGGC AATCAAGGCC 
CTGGAAGATG TCAGACGCGA TGAAGCCTAT GCGAGACGAT TACGCGATCG GCTGTGTAAC 
CTCAGGGCGG ACCCTAAATA CCTTGAGAAA TTTCACATGC GTCGCTCGAG CCTTGCAATT 
GCGTCTGTGG AGCAGCGCAT ACTTGACGCG ATGGATCAGC ACCGTGTGGT TGTCGTATGT 
GGAACAACCG GATGCGGCAA AACAACCCAG GTTCCGCAGT ACATCCTTGA TAGGGAGATT 
ATGGAGGAAC GTGGGGACCG CTGCTGCATC GTCGTGACTC AGCCCCGTCG ACTGAGTGCC 
TTCAGTATCG CGGATCGCAT TGCAAGTGAG CGACTGGACG TGGTGGGGAA AGATGTGGGG 
TATGCTGTGC GTTTGGACGC TCGGCCAGGC CGTCACATCA CGCTCTGCAC GACCGGAGTT 
TTGCTCCAGA TGTTATCCGG TATGCCATCG CTAGACACGG TGAGTCATCT TGTGATCGAC 
GAGGTGCATG AGCGTGACAT CAACTGTGAT GTGGCTCTGG CTCTTGTCAA AGAGCTGCTG 
ATGGAGGACA AAAATAAAAG GTTGAAGGTT CTTCTCATGA GTGCAACGAT GCAGTCTGAG 
ATGTTTGCTT CTTACTTTGG TGATGTGCCT GTGATATCCG TGGAGGGGGC CGTGTACCCT 
GTGAAGGTAT GCTACTTGGA CCACGTGGCC CATCTTTTTC AACATTCCTC CCCTCCCGGC 
TACTATAGTC CCATGTTTGA TGCTCTCAGC AGCGCGACCA CATCGCAGGG CCGCAACACA 
TACCGTGGCA GGGGTGGATA TCGGGGTACA AGGTGGCAAC TGACAACACC GCCAAAGACA 
GACTACGCTC TCATTGCACG TCTTATTGAA CGAAGTGTGG AGGTTGACCT TCACGGGGAG 
GTGGAAGGAA AGTCTGTCCT CGTGTTTCTC CCGGGATGGA AGGAGTTGAT AGCTGCAAAA 
CAGGCTCTCG AAGCTCTTAA CGGGGAGCAG CGGTATCATA TCATTCTCTT ACACTCCTCT 
GTGGACGCGA AGAAGCAGCG GGAGTGCTTT TCACCGGCGC CCGAAGGATT TGTGAAGGTG 
GTGCTTGCCA CAAACATTGC TGAAAGTGGT ATAACCATTG ATGACGCGGC AGTGGTGATT 
GACACCGGTC TTATCAAGCA AACCTCGTGG GTATCGCGTG CGACTGGGAC CCAACAAGAA 
CATCATGCCT CTTCGTATTC CACTCAACTT GCTTTACAGT ATGCAAGCCG CGCCAACTGT 
ACCCAACGCA AAGGCCGTGC GGGGCGCACA CAAGGTGGTA TTTGTTATCG TCTTTTTACG 
CGAGAGGTTT GGGATGTGTT GCCTTTTTTT CAGGAGGCAG AAATTCATCG TGTTCCGCTT 
ACACAAGTCC TGCTGACCCT CTTGTCACTT GGACACACAA AACCCAAAGA GACTCTTCAG 
AGGTTTATTG AACCACCTTC TGTAAAAAAT GTTGAGGCTT CCATGCGACA ACTGCGAAGC 
TTGGGGGCAG TGACTGCGGA TGAGAAGTTA ACGCCGCTTG GGTTGTATCT GTCACGGCTG 
CCATGTGACC CGCGAATTGG GAAGATGATT ATGATGGGTG CTGTGTTGCG TTGTATGGAC 
TCTGCTCTAA CGATGGCAGC GACCGCGGAT GTCAGCCCAT TTGTGACAAG CCGAGAGGTC 
TCTTTTGAGG TGCGACAAAA ACGACATGCC TTTTCGATGG GATCACAGAG CGATCACATC 
AGTGTGTTGA ATGCTTACAA TGCCTTCTGT GCTCGTCAAG GCGATGGTGT CTTTGCCAGC 
GAACAATATA TCCATCGAAA TAACATGCGG ATTATTTCCC GCTACAAGCA ACAGTACCGT 
GACATCCTCC GCCGTTCAGG ATTCATCCGG GATGGGGAAT CATTTTCTCT TGAAGCCCCT 
GAGGATGAGT TTATTATCCC CAATGATGAT GCGGTGGCTC CGCTGCATGT TGATTCCGGT 
TTACTTTCCC TTGATGCTGC AGACGTCACA CTCGTTAAGG CCTGTCTCTG TGCAGCTCTC 
TTTCCTAATG TGGCTGTGCT TGACCCTTCC CCGTTGCTTC AGGGGGGAAA AAGGGCGAAG 
ACGCTGGTGA TGCGCACTGC CACGCACAAA AACATCTCAC CTTCCAAAGA TAGTGCGTGC 
CGCCGTCTTG GACCTCCTCG TGCCCATGGT GTACTGACCC CAAAAGAGTT TTTTGGTAGA 
GATGCGGAGA GTGGTGCCGA AGCACCTGTC ATTCCCTCCA TGTTTTATAT ATACCAGAGC 
ATTTTTTGCG TGCAGGAATC CAGAGAAGAG TTTCTTACAC AGGTTTCTTC AGTGAGTCTG 
TGGGCTCTCC TGCTATTTGG TGTCGGTGAG GCGGACATGC GATTTGACCC TCTCTTGCAA 
CTCTGTATTG TGGGGGGTTG GATAGGCATA CACATCGATG CCAGCTCCTT TGCCACGCTG 
CACGCACTTC GCACTACCCT GCACACCTGC GTTTGGAGGA AATACCAGAA ACCGGATGAC 
GAGAAGAACA ATGAGGCACT TGAGGTTCTC AGGCGACTCT GCAAGGAGGT ATTACAGTCA 
CCGCCAAATG ATAAGGAGCA GTACCTGAAC CGTCTTGTCG ATACAGGGCG TATTCTCTCT 
CCGTCAATGC TGGAGCCAAT GACCACAGAC ACCAATGACG AGAATTTTGC ATGGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_821006.1
CDS	1..4200
Translation	MRRLLCKSGLLAPAREMRHERDFRGRNRQRRQRGFGEGRSSSNPRNNDDIGILQIKFHTIPALQVDMRAETQIESFLRSQLREDGSSMLLSTNASSLPIVRVINVPRSTLKRAVLEFHVRGIPVRALGQSTRAKRARALCFMHAARLIDHFSVEGTDSSAAPLPLEEGTELNAQKKYLKRVMSIRKTQAFGPPPQPEEYDCWEDYVDESRRHIERQEQERRNEAFQLLRIPHTGNPLVDRAAEMTEQERCTDRLALQKLNNELLGVSKGVNIVRISHRVFVATLTLDSVTNLVATGVAESSQEAKQRAAMHALNILTLVKEQRQASAVDGKASAFAGRGSCASPDAVSLSLTPRYSKMLDFFSLFFGVTPAPVFTREGNSYICHMDLDGVKCTGQGINRFEAERHAIENALGEMELYDERLQGINSIIAQYPNIQPRCVPTAKLPEALRNEIHAFVQRCRTELHFPVDGNTVGNEGHGGTPEKILTDDVLDAETRAAIKALEDVRRDEAYARRLRDRLCNLRADPKYLEKFHMRRSSLAIASVEQRILDAMDQHRVVVVCGTTGCGKTTQVPQYILDREIMEERGDRCCIVVTQPRRLSAFSIADRIASERLDVVGKDVGYAVRLDARPGRHITLCTTGVLLQMLSGMPSLDTVSHLVIDEVHERDINCDVALALVKELLMEDKNKRLKVLLMSATMQSEMFASYFGDVPVISVEGAVYPVKVCYLDHVAHLFQHSSPPGYYSPMFDALSSATTSQGRNTYRGRGGYRGTRWQLTTPPKTDYALIARLIERSVEVDLHGEVEGKSVLVFLPGWKELIAAKQALEALNGEQRYHIILLHSSVDAKKQRECFSPAPEGFVKVVLATNIAESGITIDDAAVVIDTGLIKQTSWVSRATGTQQEHHASSYSTQLALQYASRANCTQRKGRAGRTQGGICYRLFTREVWDVLPFFQEAEIHRVPLTQVLLTLLSLGHTKPKETLQRFIEPPSVKNVEASMRQLRSLGAVTADEKLTPLGLYLSRLPCDPRIGKMIMMGAVLRCMDSALTMAATADVSPFVTSREVSFEVRQKRHAFSMGSQSDHISVLNAYNAFCARQGDGVFASEQYIHRNNMRIISRYKQQYRDILRRSGFIRDGESFSLEAPEDEFIIPNDDAVAPLHVDSGLLSLDAADVTLVKACLCAALFPNVAVLDPSPLLQGGKRAKTLVMRTATHKNISPSKDSACRRLGPPRAHGVLTPKEFFGRDAESGAEAPVIPSMFYIYQSIFCVQESREEFLTQVSSVSLWALLLFGVGEADMRFDPLLQLCIVGGWIGIHIDASSFATLHALRTTLHTCVWRKYQKPDDEKNNEALEVLRRLCKEVLQSPPNDKEQYLNRLVDTGRILSPSMLEPMTTDTNDENFAWE

Target ORF information:

RefSeq Version	XM_815913.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener ATP-dependent RNA helicase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_815913.1

ORF Insert Sequence:

ATGCGACGAC TTTTGTGTAA ATCTGGCCTT CTGGCCCCGG CCCGGGAAAT GCGGCATGAG 
CGTGATTTCC GTGGCCGTAA TCGCCAACGC CGACAGCGTG GATTTGGCGA GGGCCGTAGC 
TCTTCCAACC CACGGAACAA TGACGACATT GGGATTTTGC AGATAAAATT TCACACCATA 
CCGGCTCTGC AGGTGGACAT GCGAGCAGAG ACTCAGATCG AGTCGTTCTT GAGATCGCAG 
CTGCGGGAGG ATGGGTCTTC GATGTTACTG TCAACGAATG CGTCATCTTT ACCCATAGTT 
CGTGTGATAA ATGTTCCCAG GTCCACCTTG AAGCGTGCCG TGCTGGAGTT CCACGTAAGG 
GGAATACCGG TGCGGGCTTT AGGGCAATCA ACCCGCGCAA AAAGGGCTAG GGCGCTCTGC 
TTCATGCATG CAGCGAGGCT CATTGATCAC TTTTCAGTAG AAGGCACGGA CAGCAGTGCT 
GCGCCTCTGC CACTCGAGGA GGGCACGGAA TTAAATGCTC AAAAGAAATA TTTGAAGCGT 
GTGATGAGTA TTCGAAAAAC GCAGGCTTTT GGTCCGCCTC CACAGCCGGA AGAATACGAT 
TGTTGGGAAG ATTATGTGGA TGAAAGTAGG CGACACATTG AACGGCAAGA GCAAGAGCGA 
CGAAACGAGG CCTTTCAGCT GCTGCGTATC CCTCATACTG GGAACCCATT GGTTGACAGG 
GCGGCGGAAA TGACCGAGCA GGAACGATGC ACCGACCGAT TGGCTCTTCA AAAACTTAAC 
AATGAACTTC TTGGGGTCAG CAAGGGTGTG AATATTGTTC GCATCTCGCA TCGTGTCTTT 
GTCGCCACAC TCACATTGGA TAGTGTGACA AATTTAGTTG CTACTGGTGT AGCTGAGAGT 
TCACAGGAGG CGAAGCAGCG TGCCGCGATG CATGCGCTTA ACATTCTTAC GCTGGTGAAG 
GAGCAACGGC AAGCTTCTGC CGTGGATGGG AAGGCATCAG CTTTTGCAGG CCGTGGAAGC 
TGTGCCTCGC CTGACGCCGT GTCACTCAGC CTTACGCCAC GTTATTCCAA GATGCTGGAC 
TTTTTTTCAC TTTTTTTTGG TGTGACTCCG GCTCCTGTCT TCACAAGGGA GGGAAATTCC 
TATATTTGTC ACATGGATTT GGACGGTGTC AAGTGCACGG GACAAGGCAT TAACCGGTTT 
GAAGCGGAGC GCCATGCCAT TGAGAACGCA TTGGGAGAGA TGGAGTTGTA TGACGAGCGG 
TTGCAGGGCA TTAATAGCAT CATTGCACAG TATCCGAACA TCCAGCCACG GTGTGTTCCG 
ACTGCCAAAT TGCCGGAGGC GTTGAGGAAT GAAATTCACG CCTTTGTACA GCGCTGTCGC 
ACCGAACTTC ATTTTCCAGT TGATGGCAAT ACGGTGGGAA ACGAAGGCCA TGGTGGTACT 
CCTGAAAAAA TTTTAACTGA TGACGTTTTG GATGCCGAGA CACGGGCGGC AATCAAGGCC 
CTGGAAGATG TCAGACGCGA TGAAGCCTAT GCGAGACGAT TACGCGATCG GCTGTGTAAC 
CTCAGGGCGG ACCCTAAATA CCTTGAGAAA TTTCACATGC GTCGCTCGAG CCTTGCAATT 
GCGTCTGTGG AGCAGCGCAT ACTTGACGCG ATGGATCAGC ACCGTGTGGT TGTCGTATGT 
GGAACAACCG GATGCGGCAA AACAACCCAG GTTCCGCAGT ACATCCTTGA TAGGGAGATT 
ATGGAGGAAC GTGGGGACCG CTGCTGCATC GTCGTGACTC AGCCCCGTCG ACTGAGTGCC 
TTCAGTATCG CGGATCGCAT TGCAAGTGAG CGACTGGACG TGGTGGGGAA AGATGTGGGG 
TATGCTGTGC GTTTGGACGC TCGGCCAGGC CGTCACATCA CGCTCTGCAC GACCGGAGTT 
TTGCTCCAGA TGTTATCCGG TATGCCATCG CTAGACACGG TGAGTCATCT TGTGATCGAC 
GAGGTGCATG AGCGTGACAT CAACTGTGAT GTGGCTCTGG CTCTTGTCAA AGAGCTGCTG 
ATGGAGGACA AAAATAAAAG GTTGAAGGTT CTTCTCATGA GTGCAACGAT GCAGTCTGAG 
ATGTTTGCTT CTTACTTTGG TGATGTGCCT GTGATATCCG TGGAGGGGGC CGTGTACCCT 
GTGAAGGTAT GCTACTTGGA CCACGTGGCC CATCTTTTTC AACATTCCTC CCCTCCCGGC 
TACTATAGTC CCATGTTTGA TGCTCTCAGC AGCGCGACCA CATCGCAGGG CCGCAACACA 
TACCGTGGCA GGGGTGGATA TCGGGGTACA AGGTGGCAAC TGACAACACC GCCAAAGACA 
GACTACGCTC TCATTGCACG TCTTATTGAA CGAAGTGTGG AGGTTGACCT TCACGGGGAG 
GTGGAAGGAA AGTCTGTCCT CGTGTTTCTC CCGGGATGGA AGGAGTTGAT AGCTGCAAAA 
CAGGCTCTCG AAGCTCTTAA CGGGGAGCAG CGGTATCATA TCATTCTCTT ACACTCCTCT 
GTGGACGCGA AGAAGCAGCG GGAGTGCTTT TCACCGGCGC CCGAAGGATT TGTGAAGGTG 
GTGCTTGCCA CAAACATTGC TGAAAGTGGT ATAACCATTG ATGACGCGGC AGTGGTGATT 
GACACCGGTC TTATCAAGCA AACCTCGTGG GTATCGCGTG CGACTGGGAC CCAACAAGAA 
CATCATGCCT CTTCGTATTC CACTCAACTT GCTTTACAGT ATGCAAGCCG CGCCAACTGT 
ACCCAACGCA AAGGCCGTGC GGGGCGCACA CAAGGTGGTA TTTGTTATCG TCTTTTTACG 
CGAGAGGTTT GGGATGTGTT GCCTTTTTTT CAGGAGGCAG AAATTCATCG TGTTCCGCTT 
ACACAAGTCC TGCTGACCCT CTTGTCACTT GGACACACAA AACCCAAAGA GACTCTTCAG 
AGGTTTATTG AACCACCTTC TGTAAAAAAT GTTGAGGCTT CCATGCGACA ACTGCGAAGC 
TTGGGGGCAG TGACTGCGGA TGAGAAGTTA ACGCCGCTTG GGTTGTATCT GTCACGGCTG 
CCATGTGACC CGCGAATTGG GAAGATGATT ATGATGGGTG CTGTGTTGCG TTGTATGGAC 
TCTGCTCTAA CGATGGCAGC GACCGCGGAT GTCAGCCCAT TTGTGACAAG CCGAGAGGTC 
TCTTTTGAGG TGCGACAAAA ACGACATGCC TTTTCGATGG GATCACAGAG CGATCACATC 
AGTGTGTTGA ATGCTTACAA TGCCTTCTGT GCTCGTCAAG GCGATGGTGT CTTTGCCAGC 
GAACAATATA TCCATCGAAA TAACATGCGG ATTATTTCCC GCTACAAGCA ACAGTACCGT 
GACATCCTCC GCCGTTCAGG ATTCATCCGG GATGGGGAAT CATTTTCTCT TGAAGCCCCT 
GAGGATGAGT TTATTATCCC CAATGATGAT GCGGTGGCTC CGCTGCATGT TGATTCCGGT 
TTACTTTCCC TTGATGCTGC AGACGTCACA CTCGTTAAGG CCTGTCTCTG TGCAGCTCTC 
TTTCCTAATG TGGCTGTGCT TGACCCTTCC CCGTTGCTTC AGGGGGGAAA AAGGGCGAAG 
ACGCTGGTGA TGCGCACTGC CACGCACAAA AACATCTCAC CTTCCAAAGA TAGTGCGTGC 
CGCCGTCTTG GACCTCCTCG TGCCCATGGT GTACTGACCC CAAAAGAGTT TTTTGGTAGA 
GATGCGGAGA GTGGTGCCGA AGCACCTGTC ATTCCCTCCA TGTTTTATAT ATACCAGAGC 
ATTTTTTGCG TGCAGGAATC CAGAGAAGAG TTTCTTACAC AGGTTTCTTC AGTGAGTCTG 
TGGGCTCTCC TGCTATTTGG TGTCGGTGAG GCGGACATGC GATTTGACCC TCTCTTGCAA 
CTCTGTATTG TGGGGGGTTG GATAGGCATA CACATCGATG CCAGCTCCTT TGCCACGCTG 
CACGCACTTC GCACTACCCT GCACACCTGC GTTTGGAGGA AATACCAGAA ACCGGATGAC 
GAGAAGAACA ATGAGGCACT TGAGGTTCTC AGGCGACTCT GCAAGGAGGT ATTACAGTCA 
CCGCCAAATG ATAAGGAGCA GTACCTGAAC CGTCTTGTCG ATACAGGGCG TATTCTCTCT 
CCGTCAATGC TGGAGCCAAT GACCACAGAC ACCAATGACG AGAATTTTGC ATGGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053506435.120 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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