Tc00.1047053508547.80 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053508547.80
Entrez Gene ID	3548938
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_811863.1	XP_816956.1	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053508547.80 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053508547.80 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb11444	XM_811863.1 Latest version!	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053508547.80 ( XM_811863.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_816956.1 Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OTb11444
Clone ID Related Accession (Same CDS sequence)	XM_811863.1
Accession Version	XM_811863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849439). COMPLETENESS: incomplete on both ends.

ATGCATTCTG GGGAAGTGTT TTTTCCGCAG CGCTGCAGTC ACCTTTCCTG CGCGTTCAAC 
GCTCTTGGGG AGCTTCGAGT GGCGATGGCC TTGGCGGATC GGTTGCTTTT TTTGAAGGAG 
GGAACGCTGT GCGCATGTGG GCCTCTTTTT AACGATCCGG GAGGGGAGCA AGTGGGAAGT 
GAAAAAAATA CGGAGGCAGC GACCTTGCGT CAACTTCGCT TTTGCCAATT TAGTGATGAA 
ATCCGAGTGG CTGTGGTGGG GACAAATGGG GTAACTGTTG TGGATGTCAG CGAGGGCGAC 
ATGCAACTGA GGCACAGACG GGTTCTGGTG GAAAGCCACC TTGGTACCTC TACGGTGAAG 
CGATGTGCAT CTTCTTTCTT TTCTTTGGCA TCTGTTTTGC CCCAGGGGGA AGCAACTTCG 
TTTTCAACGC CATCGTGGAT GTCAGGGTGG GATGCGTCAG TGCGTCGCGA GGAAGTGCGT 
GGTGCGGTGG ACGCGGAGTT TACCTCAGTT TCCTCGCTCG CCATTGTTTT GGGTGACAGG 
GCAGTGGTAG TCGAAATTGG CGCAGATGGT GACATTGTCA AGTGGCGCAG TTGGGGTTCC 
TACAGTACAG TGGCCGTTTG CCGCACGAAT CTGCATCTTG CATTTGTTGT GAAGTCCTCT 
GCTGTGGAGG TGTTTCCGCG GAGGGTGAGG CGTTGCCAGG AGGCCGCCTC GTCGCTACCT 
TCCCTGCAGG AGGAGTGTGA TCCAAGCATT CTATTCCCAC CCTCGCATGC GAAGGCCTCA 
TTCAATGGGA AGGATCCCAA AAAGTGTGAC AAATTTGCCT TGCGCGGAGT GCTAGCAAAG 
TTAGGGGTTT ACAAAGTAGC AGCAGTTGAG TGGCACCATA TCGCCGCCCA TACAATTTTG 
TGCGTCACAT GTCAACATCC AAATGACGAT CAGATTTCCA TTGCTCTTTT CGTCGTGCAG 
TTCATACCAG AGGTATGGAC ATCGCGGCAG AAGCTCGGGG AAAACAGTGA AACTGAATTA 
ACGCAACGTA AGGAGAGTGA TCACTCATTT CAGCTTGTAC CTTTTGGGAC AGTTTCTTTT 
GGTTCAATGT CGTATTCTCT AAGAGCGCAA CTCGTTCCAA GTTTTTGCCG TTCTATCCAC 
ACAAAATCCT TGAGTAACAA GCTGGAATTT CTTTGCTTCG AGGATGACGG TACAGTGCGT 
TCTGTATGCT ACCGATTTGG TCGACAGAAG TTAGGGGGAT TCCCTATGCA GCGCTCTGCC 
GGTGTTAAAG CCTCCAAATT GTTACTGCCA TTACAAGAGA ATTATGGAAA AATTGTTCGT 
GTGGATGTTC TACCGGTCTG GAAATTCCGG GCCGTTGAGC TTGGCGCCTT TACCCCTCAA 
GCAGAACAGC TTATGAGGAC GCGGAGGTAT CGTCTGCTGA CGCACTTCGC CAACGGCATG 
GTGGCGAAGG TGATGATTGA CCTTTTAACG ATGGTCATAC GTGTGGAGGC GTTCCTGACG 
CTTGGTGTCG ATTCACTGCC TCTTTGCCTC TCCACATCTC GGGGCAATGA TGGAGAGGTG 
CTGCTTTTGG GAATCTCACA CGAGCGCGCG TTTGTCATTG AGCAAGTTAC GAGAGATCGT 
GGTGTCGCTG AAACGACACT TCTTGCAGTA TCTCCAATTT CTGTGGAACA CCGCCAACGA 
ATGCAGGGGC TGCGGAAGTG CGGCGCCACG ATGGACACGT ATGACACCGT TGCTGGAGAT 
GAAAAGAAGG GCGAAGAGGA GGCTGTTAAG CTTTTTGTGG AGGCGAAGTG CCCTGAAAAA 
GCGCGTATGA TTCCGTCGCT CATGAAGGCG TGCGGTGGGG ATGCAGGGAA GCTTCTAACC 
CGGCTGGCAG CGAAATACGG CCCAATCGAG TCACCAGATG CGCCTCACAA GGAGGTTCAT 
GCCAATGATG ATCCTGGTAG TGTTTTGGAT GCACACTCGA AATTGTCTTC CTCCGCCTCC 
TCCTCCTCCT TGTTGCCACT GGAGACGGGA TTTCGGCTGC AGCATGTGCA GTTTACTGCG 
GAGGGTATCT GTTGCTCTAC GAGACATTCA ATAAACGGTA GGGAGGAGTG GTGGTGTATT 
CCTACCACCA TTCTCGAGAA CTTTTCGGCA GTGCAAAGCG AACTGGTTGT ACCCGCGGCA 
AGAATTCCTG CGATAGCACC GATGCGCTCC TTAAATGCTG CGCCCATTGA AGAACTCCGC 
CACTTTGACG ACAAGGAAGT GTTTTGCGAG TGGGTGGAAA GCCTTAAAAG CATCACGGTA 
CGGGCAAAAT CACTACCCGA TAATCCTCCA TTGTCATTTC AGCCCTTTGA AAACTTCCCA 
CGGTATCGTG TGGATGAAGT CAAGGCGACC AGGCTTGTGA ACAGGGCAGT TATTGTTGGT 
GTTATGGGCA CAATATATGG CGCGGAGACG AGAAGGGTGC TATGGCTGTA CGGACTGGAC 
CTGCTCCGTA ACGTTGCAGC ACCACCAATG TTTTTTCTGG AGTTGAAGAT GGAAGATGTG 
CTGTCGTTTG CTCTGAGTCA AAATTCCACT GTTTTTGTAC TGCACCGGCA CAAAGACGTC 
ATCGATCGGT GGATTCGGGT TGCTACGGGT GGGGAGGACG GCTTTCACTG GGAAGGGGGT 
CCTGCACACG ATGAGGTTAG TTGGCGAGGA GAAATCACGG CTATGGCCCC ATTATGGGCT 
GAAACAAAGG GTCCTAACAA GGGAGAGATG CAATTGGAGA AGCTGCTGTT TTCTTCTTCC 
GATGGCTCTT TTATTCATGC CTGGAATACT CCACCCTCTG CACCGGCGAC TTTCATGCAG 
TCGCGGATTG ATAATTTCAA CACGGATTCC ACCGTGGAGA TATATCATCC TACGACACTC 
CTTGTGCTTC TGAGAATGGG GAGGTGGCGA GCGGTGCGGT TTATACTAGA GTACGTGCTC 
CAGTTCGCTT GTGCAATGAA TGCTGATGGA GTCTCTGTCA CAGACGACAT CAACCCGAGC 
GTGTTGGCAA GGGAGTTTTG TCACAACCCA GTAAGCATCC GGCTCTGTGA GAGGTCTTCT 
GTGGCGCAAG GGGACCTGGA TGCCACCACC TTTTCCGCCG AAGTCACAAT TAATATTGAC 
TTGCAGATAG AGCGTCTCCT GGAAATGGAT GAAAAGAGTG ATGGCCCTAG TGTGTCAGAT 
AATGGATTCA CCCCGGAAAT GCTCGAGAAG GTCATGGAGC TGCTTTCCTT GGCGCAGCTT 
CGGGGCCTTA GCAGCCAAGA ACAGTTGGGT CTTGTCTGCG TTGTGGATGC CATACGAGCG 
GTGTGTTTGT TTACTATTGG CATGGACGTG GCAGCCTCAA AGTTTTTTTT CTTGCACAAA 
CTAGGCCAAC TTTGGCGACG TCTGGGCGTG CGTGAGACTT TCAAAAACAT CATTCCCCAT 
ACGCACACTG CATTCTTGTG GGCGGCGCTC TCCGACGCAC AGCCACGGTT GCTGCATGAC 
CTCCTGGACG GGAATGATGG GAAGGATGCT GTCTCGTGGA CCGATGTGGA GGCTGCCGGG 
GTGCCATTCT GGTTAAGGTC GCTGCAGGGG CTGCGGACAC TGGCGGAGCG GGTGGCACGC 
GGACAGTACC AGGCAACAAG GGATGTAAGA GAATGTGCTC TCATGTACAT TCTTGCTGGG 
AAAGTTGGTG TTCTTGCGGC CCTGTGTAAG GCGGAGCGAA ACGCGAAACT GCACGCTTTT 
TTTTTGCGAA ATTTCAATGA ATCAAAGAAT AAGACAGCCG CTGCCGGTAA TGGGTTTGCA 
GCTGTGAGCA AGAATATGGT GGCATATGGC GCGGCCTTTT TTGTTCTTGC AGGGGAGTTC 
CGAAATGCCA CTCAAGTTCT TCTGCAGCGT CACGGCAGCA TTGCACTTGC CCTGTTGGTT 
TTGCGCTTGG CCAGTAATGA CAATCGGGAT GATTTGCTTT GGTTTATTGA GCAGCGAGTC 
CGTGAGGAGG CAGAATATGG ACCGATGAAC GTCTGGGAAG AGGCGTGCCT GCTCTGGCAC 
TGCGGACTGA CCCGAGAGGC CCGTGGGTTG CTTTCACGTC ATGTACCGCT GTGCGCCGTT 
GAGGCTGCCG AAATTGTGAG CTTCTTGCGG AATGAGCGAC GGCACGTGCG TGGGGAAGGA 
CTAACTCCTT TGCGGGAGCT GCTTTTGCTT ATGCATATGA CTCGCCTCTC ACACGCCAAA 
GGGATGCAAC TGCCAGCAAT GATGAGCTGT CGCGAAGCGG AGGCGCTGCT CGCACAGGTG 
CGAGGTGCAA AGGCAGAGGA GCACAAGGCA GTGGCAGCGA AAATAACCTT TGCTACACGT 
ACCAAAGCTG ATTTTAACAC TGGCACATTG ACGTTTCACG GCTTTGGTCT GGATGATGAG 
GATGATGAGT ATGCTGGAGG AGAGCATGGG AACGATAGTA ATAACATTCA TGGCGTCGAG 
AAAGATGCAC TTGACGCAAA AGTGGCGTCT ACAGCAGTTG CACCTTCAAG AATGGTGTAT 
CACGAGGAAG AAGAGGATGC GAGTCAGATT GATGTTATCT CAGCGGTGGC ACTGGAAAGG 
GAGCTGGAGT GGGCTTGGAG CCGGCTTCAT GGCACAATTC CAGCTGATGG CTTGACAGAA 
GATAAAGACG GCGGCAGGGG TACTGTGCAT GCATTTCTCG TTTGTACGGC TCGCATGGTG 
GCGGATGTTG GCAGGCATAT GCCCATAGCT CGTGTGGAGA ATCGTTTGGC ATGTTTCTTG 
AAATCACTCT TATTGTTGCA GAACTCTCGT GCTGATCCAC TGTCCGATGA ACCCTTGCCA 
TTACCCATGC AGGGTGTTGT ATCGCGTCTT GTAATTGTCG CCGTGCGCCT GGTGCTGTTG 
TCAGTAGCAA TTCAGAATAC GGACTACGCG CTGGCGAGCG CCTTGCTGGA GTTCCCAACG 
GTGGTGAAGG TCCTTCGTGA GCAAAATTCG GAGACGGCAA CGTTGGATTC TGTCCTGTCA 
TATGTGCGCG GACTGCAAGG CGTCCTGAGG CGCGTACGGG CTATGACTAT GGAGCAACTG 
AAAGAAGAAG AGACGGGAGA GGTTGGGGCC TTTTCACCCT CTCATGAATT GTTAAACACC 
GGAAAAGATG GCGGCCCGTT GTTATGGGCT ACAATGTATG AGGAGAAACT CCGCTCGTTG 
CTGATCCTCT GGAGTGCCGC ATTCCTGCAT CTGTGTGCGC TGAGCGAGCT GCGGGAGGAG 
GTTCATTACA TACGGGGCGT CTTTGCGGAT CTCTCGCATC CACCGCAGTA CGCAAAAATC 
ATGCTGTGGT TGCTTGGCTG TATGTTGTGC CGCGTCACAT TGGCGTTCAA CACGGCCGCC 
TCACAAGTGG TTGGCCACGT GGTGGCCGTG CGGCAACTAG ACGACGGTCT TCTCACGAAC 
CCCAACGGTA TTTTCATTGA GGAGGAGCAG GAGCTGCGAC GGGTGGCGGA GTTGCTGGAA 
AGTAACCTTC TTTTCAACAT AGAGGATGCG GCAACGCAGC ATCCAGTGCG GACAGTCTTG 
CTGGACCTTT GCGTGGCCCA TGCAGAGTTT TTTTGGCAGT TTCCTAGCAT AAACGCAGCG 
ATATCCACCG ATACCGATCA AGTTGTGCTG TTGCTGCGGG ATGCGACACA ACCACACACT 
ACAGCACATC TTCTTTACGG GCAGTTGAGT GATGGCGCAG TGGCGACGCT TCGGCACATG 
GAGGTGACTT GGCTGCGGCG CGTCCACACA CATGCGGGCT TTTGTGAGAT GATGCTGGAG 
GCGGCCCTCT GCGGTAGTTT GGAGCAGATC ACAACAACAG ACCGGTTAGT GCTCTATCAA 
TCCCATCGTG CAATATGCTC TGTCGACTAC GATCGCTCCA GTTGCGAGAG CCTTGTGTGG 
GGCACCGACG CCGGTGCGGA AGTAAGTCAC GGCTACCGCG AGATATTGGC GGGCGACAAC 
GAGGCCGCGT TTCTGCGGGA GCAGCCAGAT CGAAACGTTG TGACAGCGAC CCTGTCGGCG 
GGGTTGGGCA GAGAACAGGG CAATGGGACG GCTGTGGGCG CGACCCAGCG GGACCCGAAG 
GCATTGTCGC AGCGGCCCCG TTTTTCATTT GCTTCCCACG CGATGGTCGT GTCCCACCCA 
CATCTTCCTT TTTTTCTCAC TCGTCACAGT GATGGGTGTG TGGATTTGTA CTCCTTCACC 
TCCAACGAGT GCGTGGCGAC TTTCCTCTGC CATGGAAGTC ACGTGGTGAC AGATATCGCA 
TACTCGTCCA CTGGGTACGT GTTCGCGGCA GGCCTGGCAG ACGGAAGTGT CTCTGGATGG 
CGTTTTAACC TCAGCAACTC GTCGAACGAT CCACTTTTTT CGCACCATCT CCTTCCGCCC 
GGCGGGGTGA AAGTGACGCT CTTCTTTGAA AATCAGCACT CGCTACTTAT TGTGGCTGGC 
TTTGACTACG CCAAGGAGAA GAGCTCAGCA GAGACGGCGC CGAGGGGAAG ACGGGGAGGT 
GGTGGAATTT TTTACAACAG TCGGGCGGGC GATCGCGATC AAAACTTCGT TGGTGTAATC 
CTTTTGGTGG ATCTTGTGGT TAATGGAGGT GCAGTGTTGG TACGCCGTGA GCTGCCGTTT 
GTGCCGGAGC ATGCTGTATA CATGACAGGC CTTGACCTTG TCTTGTGCGT CGCAGTCGAC 
GGCCGCGTTT TGTTGTTTGA TATATGGAAG TCACACCTGT ACATTCTCGG GAACGCACCG 
CCCGTCTCCC CGTGCATTGC TGTTTCCTGT GTTGCAGTGA GTCACTACGA CGACGTTCTT 
GCCATCGGCA CGCAGGATGG GCATGCGCTG CTACTGCGAT TTCACACCAT CCTTGAGGCA 
AAGTCCTCAT GTCTGGAGGA AGCGTCATGT GAGTCCAAAA TGGCTACATA TGTCACGGGG 
GAGAACGTGC TTTACAAAGC CTCTCGTCTG CAGCTAGCGC CAGTGCTGAA GTCGCACTCT 
GGTGTTTCTT GCATGGTTTT TGCCCCATCT GTGCTGCTGG CCGGTCTTAA CGATGGCAAA 
CTTGTTGCAG CGATGCTTGT GCCGCATGCT CTTCGTCAAG GCGGTCCACT GTCGCAGATG 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_816956.1
CDS	1..7086
Translation	MHSGEVFFPQRCSHLSCAFNALGELRVAMALADRLLFLKEGTLCACGPLFNDPGGEQVGSEKNTEAATLRQLRFCQFSDEIRVAVVGTNGVTVVDVSEGDMQLRHRRVLVESHLGTSTVKRCASSFFSLASVLPQGEATSFSTPSWMSGWDASVRREEVRGAVDAEFTSVSSLAIVLGDRAVVVEIGADGDIVKWRSWGSYSTVAVCRTNLHLAFVVKSSAVEVFPRRVRRCQEAASSLPSLQEECDPSILFPPSHAKASFNGKDPKKCDKFALRGVLAKLGVYKVAAVEWHHIAAHTILCVTCQHPNDDQISIALFVVQFIPEVWTSRQKLGENSETELTQRKESDHSFQLVPFGTVSFGSMSYSLRAQLVPSFCRSIHTKSLSNKLEFLCFEDDGTVRSVCYRFGRQKLGGFPMQRSAGVKASKLLLPLQENYGKIVRVDVLPVWKFRAVELGAFTPQAEQLMRTRRYRLLTHFANGMVAKVMIDLLTMVIRVEAFLTLGVDSLPLCLSTSRGNDGEVLLLGISHERAFVIEQVTRDRGVAETTLLAVSPISVEHRQRMQGLRKCGATMDTYDTVAGDEKKGEEEAVKLFVEAKCPEKARMIPSLMKACGGDAGKLLTRLAAKYGPIESPDAPHKEVHANDDPGSVLDAHSKLSSSASSSSLLPLETGFRLQHVQFTAEGICCSTRHSINGREEWWCIPTTILENFSAVQSELVVPAARIPAIAPMRSLNAAPIEELRHFDDKEVFCEWVESLKSITVRAKSLPDNPPLSFQPFENFPRYRVDEVKATRLVNRAVIVGVMGTIYGAETRRVLWLYGLDLLRNVAAPPMFFLELKMEDVLSFALSQNSTVFVLHRHKDVIDRWIRVATGGEDGFHWEGGPAHDEVSWRGEITAMAPLWAETKGPNKGEMQLEKLLFSSSDGSFIHAWNTPPSAPATFMQSRIDNFNTDSTVEIYHPTTLLVLLRMGRWRAVRFILEYVLQFACAMNADGVSVTDDINPSVLAREFCHNPVSIRLCERSSVAQGDLDATTFSAEVTINIDLQIERLLEMDEKSDGPSVSDNGFTPEMLEKVMELLSLAQLRGLSSQEQLGLVCVVDAIRAVCLFTIGMDVAASKFFFLHKLGQLWRRLGVRETFKNIIPHTHTAFLWAALSDAQPRLLHDLLDGNDGKDAVSWTDVEAAGVPFWLRSLQGLRTLAERVARGQYQATRDVRECALMYILAGKVGVLAALCKAERNAKLHAFFLRNFNESKNKTAAAGNGFAAVSKNMVAYGAAFFVLAGEFRNATQVLLQRHGSIALALLVLRLASNDNRDDLLWFIEQRVREEAEYGPMNVWEEACLLWHCGLTREARGLLSRHVPLCAVEAAEIVSFLRNERRHVRGEGLTPLRELLLLMHMTRLSHAKGMQLPAMMSCREAEALLAQVRGAKAEEHKAVAAKITFATRTKADFNTGTLTFHGFGLDDEDDEYAGGEHGNDSNNIHGVEKDALDAKVASTAVAPSRMVYHEEEEDASQIDVISAVALERELEWAWSRLHGTIPADGLTEDKDGGRGTVHAFLVCTARMVADVGRHMPIARVENRLACFLKSLLLLQNSRADPLSDEPLPLPMQGVVSRLVIVAVRLVLLSVAIQNTDYALASALLEFPTVVKVLREQNSETATLDSVLSYVRGLQGVLRRVRAMTMEQLKEEETGEVGAFSPSHELLNTGKDGGPLLWATMYEEKLRSLLILWSAAFLHLCALSELREEVHYIRGVFADLSHPPQYAKIMLWLLGCMLCRVTLAFNTAASQVVGHVVAVRQLDDGLLTNPNGIFIEEEQELRRVAELLESNLLFNIEDAATQHPVRTVLLDLCVAHAEFFWQFPSINAAISTDTDQVVLLLRDATQPHTTAHLLYGQLSDGAVATLRHMEVTWLRRVHTHAGFCEMMLEAALCGSLEQITTTDRLVLYQSHRAICSVDYDRSSCESLVWGTDAGAEVSHGYREILAGDNEAAFLREQPDRNVVTATLSAGLGREQGNGTAVGATQRDPKALSQRPRFSFASHAMVVSHPHLPFFLTRHSDGCVDLYSFTSNECVATFLCHGSHVVTDIAYSSTGYVFAAGLADGSVSGWRFNLSNSSNDPLFSHHLLPPGGVKVTLFFENQHSLLIVAGFDYAKEKSSAETAPRGRRGGGGIFYNSRAGDRDQNFVGVILLVDLVVNGGAVLVRRELPFVPEHAVYMTGLDLVLCVAVDGRVLLFDIWKSHLYILGNAPPVSPCIAVSCVAVSHYDDVLAIGTQDGHALLLRFHTILEAKSSCLEEASCESKMATYVTGENVLYKASRLQLAPVLKSHSGVSCMVFAPSVLLAGLNDGKLVAAMLVPHALRQGGPLSQML

Target ORF information:

RefSeq Version	XM_811863.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_811863.1

ORF Insert Sequence:

ATGCATTCTG GGGAAGTGTT TTTTCCGCAG CGCTGCAGTC ACCTTTCCTG CGCGTTCAAC 
GCTCTTGGGG AGCTTCGAGT GGCGATGGCC TTGGCGGATC GGTTGCTTTT TTTGAAGGAG 
GGAACGCTGT GCGCATGTGG GCCTCTTTTT AACGATCCGG GAGGGGAGCA AGTGGGAAGT 
GAAAAAAATA CGGAGGCAGC GACCTTGCGT CAACTTCGCT TTTGCCAATT TAGTGATGAA 
ATCCGAGTGG CTGTGGTGGG GACAAATGGG GTAACTGTTG TGGATGTCAG CGAGGGCGAC 
ATGCAACTGA GGCACAGACG GGTTCTGGTG GAAAGCCACC TTGGTACCTC TACGGTGAAG 
CGATGTGCAT CTTCTTTCTT TTCTTTGGCA TCTGTTTTGC CCCAGGGGGA AGCAACTTCG 
TTTTCAACGC CATCGTGGAT GTCAGGGTGG GATGCGTCAG TGCGTCGCGA GGAAGTGCGT 
GGTGCGGTGG ACGCGGAGTT TACCTCAGTT TCCTCGCTCG CCATTGTTTT GGGTGACAGG 
GCAGTGGTAG TCGAAATTGG CGCAGATGGT GACATTGTCA AGTGGCGCAG TTGGGGTTCC 
TACAGTACAG TGGCCGTTTG CCGCACGAAT CTGCATCTTG CATTTGTTGT GAAGTCCTCT 
GCTGTGGAGG TGTTTCCGCG GAGGGTGAGG CGTTGCCAGG AGGCCGCCTC GTCGCTACCT 
TCCCTGCAGG AGGAGTGTGA TCCAAGCATT CTATTCCCAC CCTCGCATGC GAAGGCCTCA 
TTCAATGGGA AGGATCCCAA AAAGTGTGAC AAATTTGCCT TGCGCGGAGT GCTAGCAAAG 
TTAGGGGTTT ACAAAGTAGC AGCAGTTGAG TGGCACCATA TCGCCGCCCA TACAATTTTG 
TGCGTCACAT GTCAACATCC AAATGACGAT CAGATTTCCA TTGCTCTTTT CGTCGTGCAG 
TTCATACCAG AGGTATGGAC ATCGCGGCAG AAGCTCGGGG AAAACAGTGA AACTGAATTA 
ACGCAACGTA AGGAGAGTGA TCACTCATTT CAGCTTGTAC CTTTTGGGAC AGTTTCTTTT 
GGTTCAATGT CGTATTCTCT AAGAGCGCAA CTCGTTCCAA GTTTTTGCCG TTCTATCCAC 
ACAAAATCCT TGAGTAACAA GCTGGAATTT CTTTGCTTCG AGGATGACGG TACAGTGCGT 
TCTGTATGCT ACCGATTTGG TCGACAGAAG TTAGGGGGAT TCCCTATGCA GCGCTCTGCC 
GGTGTTAAAG CCTCCAAATT GTTACTGCCA TTACAAGAGA ATTATGGAAA AATTGTTCGT 
GTGGATGTTC TACCGGTCTG GAAATTCCGG GCCGTTGAGC TTGGCGCCTT TACCCCTCAA 
GCAGAACAGC TTATGAGGAC GCGGAGGTAT CGTCTGCTGA CGCACTTCGC CAACGGCATG 
GTGGCGAAGG TGATGATTGA CCTTTTAACG ATGGTCATAC GTGTGGAGGC GTTCCTGACG 
CTTGGTGTCG ATTCACTGCC TCTTTGCCTC TCCACATCTC GGGGCAATGA TGGAGAGGTG 
CTGCTTTTGG GAATCTCACA CGAGCGCGCG TTTGTCATTG AGCAAGTTAC GAGAGATCGT 
GGTGTCGCTG AAACGACACT TCTTGCAGTA TCTCCAATTT CTGTGGAACA CCGCCAACGA 
ATGCAGGGGC TGCGGAAGTG CGGCGCCACG ATGGACACGT ATGACACCGT TGCTGGAGAT 
GAAAAGAAGG GCGAAGAGGA GGCTGTTAAG CTTTTTGTGG AGGCGAAGTG CCCTGAAAAA 
GCGCGTATGA TTCCGTCGCT CATGAAGGCG TGCGGTGGGG ATGCAGGGAA GCTTCTAACC 
CGGCTGGCAG CGAAATACGG CCCAATCGAG TCACCAGATG CGCCTCACAA GGAGGTTCAT 
GCCAATGATG ATCCTGGTAG TGTTTTGGAT GCACACTCGA AATTGTCTTC CTCCGCCTCC 
TCCTCCTCCT TGTTGCCACT GGAGACGGGA TTTCGGCTGC AGCATGTGCA GTTTACTGCG 
GAGGGTATCT GTTGCTCTAC GAGACATTCA ATAAACGGTA GGGAGGAGTG GTGGTGTATT 
CCTACCACCA TTCTCGAGAA CTTTTCGGCA GTGCAAAGCG AACTGGTTGT ACCCGCGGCA 
AGAATTCCTG CGATAGCACC GATGCGCTCC TTAAATGCTG CGCCCATTGA AGAACTCCGC 
CACTTTGACG ACAAGGAAGT GTTTTGCGAG TGGGTGGAAA GCCTTAAAAG CATCACGGTA 
CGGGCAAAAT CACTACCCGA TAATCCTCCA TTGTCATTTC AGCCCTTTGA AAACTTCCCA 
CGGTATCGTG TGGATGAAGT CAAGGCGACC AGGCTTGTGA ACAGGGCAGT TATTGTTGGT 
GTTATGGGCA CAATATATGG CGCGGAGACG AGAAGGGTGC TATGGCTGTA CGGACTGGAC 
CTGCTCCGTA ACGTTGCAGC ACCACCAATG TTTTTTCTGG AGTTGAAGAT GGAAGATGTG 
CTGTCGTTTG CTCTGAGTCA AAATTCCACT GTTTTTGTAC TGCACCGGCA CAAAGACGTC 
ATCGATCGGT GGATTCGGGT TGCTACGGGT GGGGAGGACG GCTTTCACTG GGAAGGGGGT 
CCTGCACACG ATGAGGTTAG TTGGCGAGGA GAAATCACGG CTATGGCCCC ATTATGGGCT 
GAAACAAAGG GTCCTAACAA GGGAGAGATG CAATTGGAGA AGCTGCTGTT TTCTTCTTCC 
GATGGCTCTT TTATTCATGC CTGGAATACT CCACCCTCTG CACCGGCGAC TTTCATGCAG 
TCGCGGATTG ATAATTTCAA CACGGATTCC ACCGTGGAGA TATATCATCC TACGACACTC 
CTTGTGCTTC TGAGAATGGG GAGGTGGCGA GCGGTGCGGT TTATACTAGA GTACGTGCTC 
CAGTTCGCTT GTGCAATGAA TGCTGATGGA GTCTCTGTCA CAGACGACAT CAACCCGAGC 
GTGTTGGCAA GGGAGTTTTG TCACAACCCA GTAAGCATCC GGCTCTGTGA GAGGTCTTCT 
GTGGCGCAAG GGGACCTGGA TGCCACCACC TTTTCCGCCG AAGTCACAAT TAATATTGAC 
TTGCAGATAG AGCGTCTCCT GGAAATGGAT GAAAAGAGTG ATGGCCCTAG TGTGTCAGAT 
AATGGATTCA CCCCGGAAAT GCTCGAGAAG GTCATGGAGC TGCTTTCCTT GGCGCAGCTT 
CGGGGCCTTA GCAGCCAAGA ACAGTTGGGT CTTGTCTGCG TTGTGGATGC CATACGAGCG 
GTGTGTTTGT TTACTATTGG CATGGACGTG GCAGCCTCAA AGTTTTTTTT CTTGCACAAA 
CTAGGCCAAC TTTGGCGACG TCTGGGCGTG CGTGAGACTT TCAAAAACAT CATTCCCCAT 
ACGCACACTG CATTCTTGTG GGCGGCGCTC TCCGACGCAC AGCCACGGTT GCTGCATGAC 
CTCCTGGACG GGAATGATGG GAAGGATGCT GTCTCGTGGA CCGATGTGGA GGCTGCCGGG 
GTGCCATTCT GGTTAAGGTC GCTGCAGGGG CTGCGGACAC TGGCGGAGCG GGTGGCACGC 
GGACAGTACC AGGCAACAAG GGATGTAAGA GAATGTGCTC TCATGTACAT TCTTGCTGGG 
AAAGTTGGTG TTCTTGCGGC CCTGTGTAAG GCGGAGCGAA ACGCGAAACT GCACGCTTTT 
TTTTTGCGAA ATTTCAATGA ATCAAAGAAT AAGACAGCCG CTGCCGGTAA TGGGTTTGCA 
GCTGTGAGCA AGAATATGGT GGCATATGGC GCGGCCTTTT TTGTTCTTGC AGGGGAGTTC 
CGAAATGCCA CTCAAGTTCT TCTGCAGCGT CACGGCAGCA TTGCACTTGC CCTGTTGGTT 
TTGCGCTTGG CCAGTAATGA CAATCGGGAT GATTTGCTTT GGTTTATTGA GCAGCGAGTC 
CGTGAGGAGG CAGAATATGG ACCGATGAAC GTCTGGGAAG AGGCGTGCCT GCTCTGGCAC 
TGCGGACTGA CCCGAGAGGC CCGTGGGTTG CTTTCACGTC ATGTACCGCT GTGCGCCGTT 
GAGGCTGCCG AAATTGTGAG CTTCTTGCGG AATGAGCGAC GGCACGTGCG TGGGGAAGGA 
CTAACTCCTT TGCGGGAGCT GCTTTTGCTT ATGCATATGA CTCGCCTCTC ACACGCCAAA 
GGGATGCAAC TGCCAGCAAT GATGAGCTGT CGCGAAGCGG AGGCGCTGCT CGCACAGGTG 
CGAGGTGCAA AGGCAGAGGA GCACAAGGCA GTGGCAGCGA AAATAACCTT TGCTACACGT 
ACCAAAGCTG ATTTTAACAC TGGCACATTG ACGTTTCACG GCTTTGGTCT GGATGATGAG 
GATGATGAGT ATGCTGGAGG AGAGCATGGG AACGATAGTA ATAACATTCA TGGCGTCGAG 
AAAGATGCAC TTGACGCAAA AGTGGCGTCT ACAGCAGTTG CACCTTCAAG AATGGTGTAT 
CACGAGGAAG AAGAGGATGC GAGTCAGATT GATGTTATCT CAGCGGTGGC ACTGGAAAGG 
GAGCTGGAGT GGGCTTGGAG CCGGCTTCAT GGCACAATTC CAGCTGATGG CTTGACAGAA 
GATAAAGACG GCGGCAGGGG TACTGTGCAT GCATTTCTCG TTTGTACGGC TCGCATGGTG 
GCGGATGTTG GCAGGCATAT GCCCATAGCT CGTGTGGAGA ATCGTTTGGC ATGTTTCTTG 
AAATCACTCT TATTGTTGCA GAACTCTCGT GCTGATCCAC TGTCCGATGA ACCCTTGCCA 
TTACCCATGC AGGGTGTTGT ATCGCGTCTT GTAATTGTCG CCGTGCGCCT GGTGCTGTTG 
TCAGTAGCAA TTCAGAATAC GGACTACGCG CTGGCGAGCG CCTTGCTGGA GTTCCCAACG 
GTGGTGAAGG TCCTTCGTGA GCAAAATTCG GAGACGGCAA CGTTGGATTC TGTCCTGTCA 
TATGTGCGCG GACTGCAAGG CGTCCTGAGG CGCGTACGGG CTATGACTAT GGAGCAACTG 
AAAGAAGAAG AGACGGGAGA GGTTGGGGCC TTTTCACCCT CTCATGAATT GTTAAACACC 
GGAAAAGATG GCGGCCCGTT GTTATGGGCT ACAATGTATG AGGAGAAACT CCGCTCGTTG 
CTGATCCTCT GGAGTGCCGC ATTCCTGCAT CTGTGTGCGC TGAGCGAGCT GCGGGAGGAG 
GTTCATTACA TACGGGGCGT CTTTGCGGAT CTCTCGCATC CACCGCAGTA CGCAAAAATC 
ATGCTGTGGT TGCTTGGCTG TATGTTGTGC CGCGTCACAT TGGCGTTCAA CACGGCCGCC 
TCACAAGTGG TTGGCCACGT GGTGGCCGTG CGGCAACTAG ACGACGGTCT TCTCACGAAC 
CCCAACGGTA TTTTCATTGA GGAGGAGCAG GAGCTGCGAC GGGTGGCGGA GTTGCTGGAA 
AGTAACCTTC TTTTCAACAT AGAGGATGCG GCAACGCAGC ATCCAGTGCG GACAGTCTTG 
CTGGACCTTT GCGTGGCCCA TGCAGAGTTT TTTTGGCAGT TTCCTAGCAT AAACGCAGCG 
ATATCCACCG ATACCGATCA AGTTGTGCTG TTGCTGCGGG ATGCGACACA ACCACACACT 
ACAGCACATC TTCTTTACGG GCAGTTGAGT GATGGCGCAG TGGCGACGCT TCGGCACATG 
GAGGTGACTT GGCTGCGGCG CGTCCACACA CATGCGGGCT TTTGTGAGAT GATGCTGGAG 
GCGGCCCTCT GCGGTAGTTT GGAGCAGATC ACAACAACAG ACCGGTTAGT GCTCTATCAA 
TCCCATCGTG CAATATGCTC TGTCGACTAC GATCGCTCCA GTTGCGAGAG CCTTGTGTGG 
GGCACCGACG CCGGTGCGGA AGTAAGTCAC GGCTACCGCG AGATATTGGC GGGCGACAAC 
GAGGCCGCGT TTCTGCGGGA GCAGCCAGAT CGAAACGTTG TGACAGCGAC CCTGTCGGCG 
GGGTTGGGCA GAGAACAGGG CAATGGGACG GCTGTGGGCG CGACCCAGCG GGACCCGAAG 
GCATTGTCGC AGCGGCCCCG TTTTTCATTT GCTTCCCACG CGATGGTCGT GTCCCACCCA 
CATCTTCCTT TTTTTCTCAC TCGTCACAGT GATGGGTGTG TGGATTTGTA CTCCTTCACC 
TCCAACGAGT GCGTGGCGAC TTTCCTCTGC CATGGAAGTC ACGTGGTGAC AGATATCGCA 
TACTCGTCCA CTGGGTACGT GTTCGCGGCA GGCCTGGCAG ACGGAAGTGT CTCTGGATGG 
CGTTTTAACC TCAGCAACTC GTCGAACGAT CCACTTTTTT CGCACCATCT CCTTCCGCCC 
GGCGGGGTGA AAGTGACGCT CTTCTTTGAA AATCAGCACT CGCTACTTAT TGTGGCTGGC 
TTTGACTACG CCAAGGAGAA GAGCTCAGCA GAGACGGCGC CGAGGGGAAG ACGGGGAGGT 
GGTGGAATTT TTTACAACAG TCGGGCGGGC GATCGCGATC AAAACTTCGT TGGTGTAATC 
CTTTTGGTGG ATCTTGTGGT TAATGGAGGT GCAGTGTTGG TACGCCGTGA GCTGCCGTTT 
GTGCCGGAGC ATGCTGTATA CATGACAGGC CTTGACCTTG TCTTGTGCGT CGCAGTCGAC 
GGCCGCGTTT TGTTGTTTGA TATATGGAAG TCACACCTGT ACATTCTCGG GAACGCACCG 
CCCGTCTCCC CGTGCATTGC TGTTTCCTGT GTTGCAGTGA GTCACTACGA CGACGTTCTT 
GCCATCGGCA CGCAGGATGG GCATGCGCTG CTACTGCGAT TTCACACCAT CCTTGAGGCA 
AAGTCCTCAT GTCTGGAGGA AGCGTCATGT GAGTCCAAAA TGGCTACATA TGTCACGGGG 
GAGAACGTGC TTTACAAAGC CTCTCGTCTG CAGCTAGCGC CAGTGCTGAA GTCGCACTCT 
GGTGTTTCTT GCATGGTTTT TGCCCCATCT GTGCTGCTGG CCGGTCTTAA CGATGGCAAA 
CTTGTTGCAG CGATGCTTGT GCCGCATGCT CTTCGTCAAG GCGGTCCACT GTCGCAGATG 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053508547.80 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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gRNAs

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Target ORF information:

ORF Insert Sequence:

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