Tc00.1047053508827.100 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

The following Tc00.1047053508827.100 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053508827.100 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OTb09375 XM_809651.1
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Trypanosoma cruzi strain CL Brener splicing factor 3B subunit 1 partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OTb09375
    Clone ID Related Accession (Same CDS sequence) XM_809651.1
    Accession Version XM_809651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3309bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1225382400000
    Organism Trypanosoma cruzi strain CL Brener
    Product splicing factor 3B subunit 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849513). COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACGGACG AAGAAAGAAG CGGCGAACCC GTACTAAAGT CACATCGCTG GGAAACAGCA 
    GCTGGCGCCC CGGCATCGTT GCCGACATCA TCACGCTGGG GAGCTGGGAC ACCGCGTCAG
    TTGGGTGGTG ACACACCGAA GGTAATCGTG AACGCTGAGC TCTCGGCGTG GCGTGGCCAC
    TCAACACCGA CGCCAAGTGC CTACATGGTA GATTCCGTCA AGACACCGAC GATGCGGGGA
    CAGGGCGGAC AGACACCTAC GTTTGGTGCT GGGGGCACCA CGCCCATGCT TGGAGGGACG
    ACACCGATGT TTGGAGGCAC AACACCGATG TTTGGAGGGA CGACACCGAT GTTTGGAGGC
    ACAACACCGG CGGCGGGAAT GGCATTTGGA GTCACCCCAA ACCATCAGTT TGAGGGAACC
    ACGCCTGTGC AGTCACGCTT TGCCGGAGGG GACTCACAAT CGGCCATCAC AGCCAACATT
    GAGGCGCAGG CCAGGAAGCT GGAGGTGCAG TGGCGGATGA AAAACAAACG ACTGACGGAG
    GAGTACCTCG ACACTATTCT CCCGCCCGAG TTCAAACTTG TAGAAGCACC CGCTGACTAC
    AATCCACCAC CTCCTGAGGA GCCAAATTTT TACGAGCTTG CCAGCAAGTC GTTGGATGTG
    TTTGTGGTGA ATCAAAGTAA CGATGCAGCG GCAGCCGTCA CATATGACAT TCCGGAGAGT
    CTTGGAGAAG GGATGCCGCA AATGAAACCG CAAGATGCAC CGGTGTTCGA GATGCTTCTT
    AAGTACCACA ATGTGAATCC CATTCCAGAT GAAGTTCTGC CTTCTTATCT GCTCATGAAG
    AACCTCTTCA GGATCAAGAA CGGTGATACA AATCAACGAC GTATTGCCAT GCGATTCTTA
    CTAGACAAGG CCCGTATCTT TGGTAGTGAG CCACTTTTTC AATTTACCTT TCATGTTTGG
    CGATCGGGAA TTCTCGACTT GCAGGAGCAA CACCATTTTG TGGATTTTGT AAAAGGAGTG
    ATTGCGAGGC TGCAGAAGGA GGTGCGGAGT TCCACAAAGG AGATTGTGCA CATGATGGAG
    GTTCTTCTCT CAGCCCAGGA GGCCGCTGTG CGAGAGGATG GTAAGGATGT CCTTGCGCTT
    CTCACCCGTG TGGTTGGGTA CGAGGCCGTG TTTGAGGCGA TCAAGGAGGA CTTTGCACAT
    GTCGAGAACG GTGTGCGTCG TCACACGGCG AAGGTGGTTG CCATTGTGGG ATACGCTGTG
    GGTCCGGCAA CTGCGCTGCA GATTATTCAC GGCATGTCAC TTTCCCCTGT CGCTTTGGCG
    AGACAAACAT CTGCACGTGC CATTACAGAA TTGGCTTCCA TCCTTATGCA TGCCATCACC
    GCTGAACTCG TGGAACTGGT GCCCATATTT GAGAAGTTAC TGCGTGATGA GCCACGTGTG
    AAGCGTGAGG CCGCTAACGC GCTTGCACAT GTTGCGGATG CGACATGTCC GTACGGCATC
    GAGGAGTTAA CGCCGTTGGT GCATATTGTA AGAGAGGAAT GTAAACGTGG CATCGGCACT
    ACCGCAGGGC CTTTTGTTCG TGCCTTTGGT TCACTTGTTC CGCTCATGGC GCCGTACGAT
    GCACAGAAGT ATACGTCCGA CATGATGCCG ACGCTTGTGA ATCAATTTAA CACTCCAGAG
    GATGAGCATC GGCGTGTGTT GTTGCAGGTT GTACGGCAGT GTGTTTCTGC GGATGGTGTT
    ACCGCAAACT TTATTCGGGA TGTCATTTTA AAGCCTTTTT TTGATGGCTT TTGGTCCGTG
    CGTCGTGTTG CTGCGGAGCG CAAGACGGCG GGTGCCCTCG TTGAGACAAC TGTGGCGATT
    TCAAGGAAAC TTGGGAGCAC GGAGATATTA CAGTACCTTG TGCAGGACAT GAAGGATGAG
    AATGAGCACT TTCAACGCAT GGTCATTGAC ACCGTGCGGC GTGTGGTCCG TGCCGTTGGG
    ACGGTTGGTG TTCCCGATAC TCTTGTTGCC CTTCTGCTCG ATGGCGCCAT TGCCGCAGTG
    AAGCAGGACG AAAGCGGACT GAATCGTGTG GTGCTGGAGG GGCTGGCCTC CATCTGCAAC
    TCACTCGGGA CACGATTGAA GCGGTACTTG CGGCAGGTAT TTGATCTTAT AAAGAGCCGT
    CGTGATATGC CCGGAATGAT CCGTATGCAG GCAGCAGAGC TGGCAGCTCG CATTGCACAC
    ACCGTGAAGG AGGCCGAGGG CGTGCTTTTC CTGCAGGATC TTGGGCGAAG CCTTTTTGAC
    CGCCTTGAAG ACGACGAGGT TGCCGTGATG AGTGCAAACA TTAAGGCCAC ACGTGCCATT
    TTAGTGGAGC TTACTGCAGC AAAGTACCAG CCTTCTGTGC GAGAGCTGCT AAAGAAATTG
    ACGTACATAA TTCGCAACCG TAACAGTAAC GTGCAGCTGA ACACAATTTT ACTCATTGAG
    GAGATTGCGA CAAACTGTGA TGAGGACGTG GATGCCATTC AGCTACAAGA TTTAGCCACA
    AAAGGATTGT TTGAGCTTCT TGACGCTGAT CGCCGTGAAA CGCGTCGTGC GTGTACCCGT
    ACATTTGGCA TTATTGCCCA GAAGATTCGT CCTTTTGCCA TCATTCTCGA ACTTGTCGAT
    AATTTCAAAC AGGACAAACG TAAGATCCGT ATCTGCACCG CTGTGGCGTT GGGGGCTATT
    GCCCGTGAGT GCGGCCCCTT CACCGTGATC CCGTACATCC TGAACGAGTA CAAGATCAGC
    GAGGGAGAGC AGGTGGCGAC GATTGTGCAA CACGCCGTCC TGAAGGCGAT TCGGTACATC
    TTTGAGGCGA TTGGGGCGGT AGGGAAGGAG TACGTGTACC CGCTCATACC ACTCCTCGTG
    CGGGCCCTCA CAGAGACGGA GATTCAGCAC CGTCGCATGG CGGTGGAGGC GTGTCGGGCA
    ATGACGCTGG CTGTGGCCGG CAACGACGGG TTCGAGGAGG TGATCGTCCA CTTCCTCAAT
    TTTATCCACC CCAACATTGT GGAGCTTCTT TCACGGAATG AGACAAAAAT CAGCGAAGAA
    CGGCTTAAGA TGGTCACTGC TGTTGTAAGT TTCTACGAGG CCGCCCGCCT TGTGATTGGG
    CCAGCCAGAC TGCTACAGTA TCTCCTGCAG GGCCTGTTTC ATCCAGCGAA AAAGGTGCGG
    GACATATACA GGAGGACATA CAACATGATC TACATTGTAA GTCCAGAGGC CCTCGTGCCG
    TGTTATCCCA GACTGGAGGA TGATGAAGAG CACACGTACG CCCGTCATGA ACTGGAGGTG
    ATTCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_814744.1
    CDS1..3309
    Translation

    Target ORF information:

    RefSeq Version XM_809651.1
    Organism Trypanosoma cruzi strain CL Brener
    Definition Trypanosoma cruzi strain CL Brener splicing factor 3B subunit 1 partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_809651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGACGGACG AAGAAAGAAG CGGCGAACCC GTACTAAAGT CACATCGCTG GGAAACAGCA 
    GCTGGCGCCC CGGCATCGTT GCCGACATCA TCACGCTGGG GAGCTGGGAC ACCGCGTCAG
    TTGGGTGGTG ACACACCGAA GGTAATCGTG AACGCTGAGC TCTCGGCGTG GCGTGGCCAC
    TCAACACCGA CGCCAAGTGC CTACATGGTA GATTCCGTCA AGACACCGAC GATGCGGGGA
    CAGGGCGGAC AGACACCTAC GTTTGGTGCT GGGGGCACCA CGCCCATGCT TGGAGGGACG
    ACACCGATGT TTGGAGGCAC AACACCGATG TTTGGAGGGA CGACACCGAT GTTTGGAGGC
    ACAACACCGG CGGCGGGAAT GGCATTTGGA GTCACCCCAA ACCATCAGTT TGAGGGAACC
    ACGCCTGTGC AGTCACGCTT TGCCGGAGGG GACTCACAAT CGGCCATCAC AGCCAACATT
    GAGGCGCAGG CCAGGAAGCT GGAGGTGCAG TGGCGGATGA AAAACAAACG ACTGACGGAG
    GAGTACCTCG ACACTATTCT CCCGCCCGAG TTCAAACTTG TAGAAGCACC CGCTGACTAC
    AATCCACCAC CTCCTGAGGA GCCAAATTTT TACGAGCTTG CCAGCAAGTC GTTGGATGTG
    TTTGTGGTGA ATCAAAGTAA CGATGCAGCG GCAGCCGTCA CATATGACAT TCCGGAGAGT
    CTTGGAGAAG GGATGCCGCA AATGAAACCG CAAGATGCAC CGGTGTTCGA GATGCTTCTT
    AAGTACCACA ATGTGAATCC CATTCCAGAT GAAGTTCTGC CTTCTTATCT GCTCATGAAG
    AACCTCTTCA GGATCAAGAA CGGTGATACA AATCAACGAC GTATTGCCAT GCGATTCTTA
    CTAGACAAGG CCCGTATCTT TGGTAGTGAG CCACTTTTTC AATTTACCTT TCATGTTTGG
    CGATCGGGAA TTCTCGACTT GCAGGAGCAA CACCATTTTG TGGATTTTGT AAAAGGAGTG
    ATTGCGAGGC TGCAGAAGGA GGTGCGGAGT TCCACAAAGG AGATTGTGCA CATGATGGAG
    GTTCTTCTCT CAGCCCAGGA GGCCGCTGTG CGAGAGGATG GTAAGGATGT CCTTGCGCTT
    CTCACCCGTG TGGTTGGGTA CGAGGCCGTG TTTGAGGCGA TCAAGGAGGA CTTTGCACAT
    GTCGAGAACG GTGTGCGTCG TCACACGGCG AAGGTGGTTG CCATTGTGGG ATACGCTGTG
    GGTCCGGCAA CTGCGCTGCA GATTATTCAC GGCATGTCAC TTTCCCCTGT CGCTTTGGCG
    AGACAAACAT CTGCACGTGC CATTACAGAA TTGGCTTCCA TCCTTATGCA TGCCATCACC
    GCTGAACTCG TGGAACTGGT GCCCATATTT GAGAAGTTAC TGCGTGATGA GCCACGTGTG
    AAGCGTGAGG CCGCTAACGC GCTTGCACAT GTTGCGGATG CGACATGTCC GTACGGCATC
    GAGGAGTTAA CGCCGTTGGT GCATATTGTA AGAGAGGAAT GTAAACGTGG CATCGGCACT
    ACCGCAGGGC CTTTTGTTCG TGCCTTTGGT TCACTTGTTC CGCTCATGGC GCCGTACGAT
    GCACAGAAGT ATACGTCCGA CATGATGCCG ACGCTTGTGA ATCAATTTAA CACTCCAGAG
    GATGAGCATC GGCGTGTGTT GTTGCAGGTT GTACGGCAGT GTGTTTCTGC GGATGGTGTT
    ACCGCAAACT TTATTCGGGA TGTCATTTTA AAGCCTTTTT TTGATGGCTT TTGGTCCGTG
    CGTCGTGTTG CTGCGGAGCG CAAGACGGCG GGTGCCCTCG TTGAGACAAC TGTGGCGATT
    TCAAGGAAAC TTGGGAGCAC GGAGATATTA CAGTACCTTG TGCAGGACAT GAAGGATGAG
    AATGAGCACT TTCAACGCAT GGTCATTGAC ACCGTGCGGC GTGTGGTCCG TGCCGTTGGG
    ACGGTTGGTG TTCCCGATAC TCTTGTTGCC CTTCTGCTCG ATGGCGCCAT TGCCGCAGTG
    AAGCAGGACG AAAGCGGACT GAATCGTGTG GTGCTGGAGG GGCTGGCCTC CATCTGCAAC
    TCACTCGGGA CACGATTGAA GCGGTACTTG CGGCAGGTAT TTGATCTTAT AAAGAGCCGT
    CGTGATATGC CCGGAATGAT CCGTATGCAG GCAGCAGAGC TGGCAGCTCG CATTGCACAC
    ACCGTGAAGG AGGCCGAGGG CGTGCTTTTC CTGCAGGATC TTGGGCGAAG CCTTTTTGAC
    CGCCTTGAAG ACGACGAGGT TGCCGTGATG AGTGCAAACA TTAAGGCCAC ACGTGCCATT
    TTAGTGGAGC TTACTGCAGC AAAGTACCAG CCTTCTGTGC GAGAGCTGCT AAAGAAATTG
    ACGTACATAA TTCGCAACCG TAACAGTAAC GTGCAGCTGA ACACAATTTT ACTCATTGAG
    GAGATTGCGA CAAACTGTGA TGAGGACGTG GATGCCATTC AGCTACAAGA TTTAGCCACA
    AAAGGATTGT TTGAGCTTCT TGACGCTGAT CGCCGTGAAA CGCGTCGTGC GTGTACCCGT
    ACATTTGGCA TTATTGCCCA GAAGATTCGT CCTTTTGCCA TCATTCTCGA ACTTGTCGAT
    AATTTCAAAC AGGACAAACG TAAGATCCGT ATCTGCACCG CTGTGGCGTT GGGGGCTATT
    GCCCGTGAGT GCGGCCCCTT CACCGTGATC CCGTACATCC TGAACGAGTA CAAGATCAGC
    GAGGGAGAGC AGGTGGCGAC GATTGTGCAA CACGCCGTCC TGAAGGCGAT TCGGTACATC
    TTTGAGGCGA TTGGGGCGGT AGGGAAGGAG TACGTGTACC CGCTCATACC ACTCCTCGTG
    CGGGCCCTCA CAGAGACGGA GATTCAGCAC CGTCGCATGG CGGTGGAGGC GTGTCGGGCA
    ATGACGCTGG CTGTGGCCGG CAACGACGGG TTCGAGGAGG TGATCGTCCA CTTCCTCAAT
    TTTATCCACC CCAACATTGT GGAGCTTCTT TCACGGAATG AGACAAAAAT CAGCGAAGAA
    CGGCTTAAGA TGGTCACTGC TGTTGTAAGT TTCTACGAGG CCGCCCGCCT TGTGATTGGG
    CCAGCCAGAC TGCTACAGTA TCTCCTGCAG GGCCTGTTTC ATCCAGCGAA AAAGGTGCGG
    GACATATACA GGAGGACATA CAACATGATC TACATTGTAA GTCCAGAGGC CCTCGTGCCG
    TGTTATCCCA GACTGGAGGA TGATGAAGAG CACACGTACG CCCGTCATGA ACTGGAGGTG
    ATTCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
    Science (New York, N.Y.)309(5733)409-15(2005 Jul)
    El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B


    Comparative genomics of trypanosomatid parasitic protozoa.
    Science (New York, N.Y.)309(5733)404-9(2005 Jul)
    El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N