Tb927.2.2370 cDNA ORF clone, Trypanosoma brucei brucei TREU927

Gene
Clones
gRNAs

Summary

Gene Symbol	Tb927.2.2370
Entrez Gene ID	3655537
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Trypanosoma brucei brucei TREU927

mRNA and Protein(s)

mRNA	Protein	Name
XM_946447.1	XP_951540.1	hypothetical protein, conserved

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

The genome of the African trypanosome Trypanosoma brucei.
Berriman M, Ghedin E, Hertz-Fowler C, Blandin G, Renauld H, Bartholomeu DC, Lennard NJ, Caler E, Hamlin NE, Haas B, Böhme U, Hannick L, Aslett MA, Shallom J, Marcello L, Hou L, Wickstead B, Alsmark UC, Arrowsmith C, Atkin RJ, Barron AJ, Bringaud F, Brooks K, Carrington M, Cherevach I, Chillingworth TJ, Churcher C, Clark LN, Corton CH, Cronin A, Davies RM, Doggett J, Djikeng A, Feldblyum T, Field MC, Fraser A, Goodhead I, Hance Z, Harper D, Harris BR, Hauser H, Hostetler J, Ivens A, Jagels K, Johnson D, Johnson J, Jones K, Kerhornou AX, Koo H, Larke N, Landfear S, Larkin C, Leech V, Line A, Lord A, Macleod A, Mooney PJ, Moule S, Martin DM, Morgan GW, Mungall K, Norbertczak H, Ormond D, Pai G, Peacock CS, Peterson J, Quail MA, Rabbinowitsch E, Rajandream MA, Reitter C, Salzberg SL, Sanders M, Schobel S, Sharp S, Simmonds M, Simpson AJ, Tallon L, Turner CM, Tait A, Tivey AR, Van Aken S, Walker D, Wanless D, Wang S, White B, White O, Whitehead S, Woodward J, Wortman J, Adams MD, Embley TM, Gull K, Ullu E, Barry JD, Fairlamb AH, Opperdoes F, Barrell BG, Donelson JE, Hall N, Fraser CM, Melville SE, El-Sayed NM
Science (New York, N.Y.)309(5733)416-22(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tb927.2.2370 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tb927.2.2370 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr135905	XM_946447.1 Latest version!	Trypanosoma brucei brucei TREU927 hypothetical protein, conserved (Tb927.2.2370), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tb927.2.2370 ( XM_946447.1 ) cDNA ORF clone, Trypanosoma brucei brucei TREU927 -> XP_951540.1 Trypanosoma brucei brucei TREU927 hypothetical protein, conserved (Tb927.2.2370), partial mRNA.

Price & Availability↑

CloneID	OTr135905
Clone ID Related Accession (Same CDS sequence)	XM_946447.1
Accession Version	XM_946447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1523203200000
Organism	Trypanosoma brucei brucei TREU927
Product	hypothetical protein, conserved
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_005063). COMPLETENESS: incomplete on both ends.

ATGTCATCGG AGGAAGCCAT TCAGGAAGTG CGACGCATTT TCACCAACAA TAGCGTGGAT 
CAGGCCCGCG ACTACCTGAG CCGTATAACA CCAGGCATTC AAGAAAGTGA TGAGAGACTC 
AAACAACTTG TCGGCCAAAG CTACCGGGGC CTCGTTGCCG CTTGTGACCA GGTGGTCGTC 
GTAGAACAGG CATGCAGGAA GTTGCTGCGT CTGCAAAAGC AAATGCAGGA GGAGAAAAAA 
CAGCAACAGT TGGAGAATCA ATATTCACAG TCGCGTCTGA CAATGCCGCA GAGCCCAAGG 
GGGGTCTCGT CACCCGTTTT GCGGACCATA TCCCCCCATG GAGGTAAAAG TCCGCGCGCG 
AAGAATGTGG GAGAAGGGAA AACTCAAACG AGTCGACATA TGGGACACTC AAGCAGTGCT 
TCGCCACCGG TTTCCGAGGT GGTGAGGTCC CCGTTGCCTC ATGAGGCAAC CGCGACTTTG 
AAGGGTGTGT GTGAAGAAAC GCCAAGAGGG GGGCGTGAGG GGGCAGATGA TAGACAACGA 
GACGTGTTAA TTTACACTCA GCAGTGCGCG GCTCTGGAGG GATTGTTGGG TGCCCGTAAG 
CTTTATGACG CCGCTGCGCT GCTCTTGCAG ATAAGAAGGG AACGGAAACA GCCTCATGAA 
AAGCTGGCTG TTGAGTCCTA TGAACATCGT CTCAAACGTG CAGTGCGTGG GCTGCTTCGG 
GAAGATGCAG AACAGGCCGC ACAAAGCGCT TTGAGTGTGG CTAACGCCAA TCGAAATGAT 
AGCGGTGGTG GGGACTTATC CTTGACATCG TCCACAAACA TGTTTACATC ATCAGGTGGT 
GTTGAATTCA TGGGTAGGGC AACAGGTTGG AAAAGTGATT TATCACGAGC ACTGGCTCAC 
ATGACGGAGG TCCACAAAGC CTTAGGAGTG CTTTCGCACG CAACAGTGAA TGACGCACTG 
TTACTCCTTA TCGATACGAT TCACGATATG ATTAAGACTG ACCTTGACCA CGTCGTGGCT 
GCACTGAAGG GAACGTCATT TACGCAGGCA GCGGCTGTAG ACACTAACAC TGACGATGGA 
AGAAGTTTAC GCCCACTTCG CAGAATGGAG TGGCTGCGGG CAGCCCTGGA GCTTCCGCCT 
TACTTAAGAA GCGAGGGAAC AAGTGCCCCA AGGGTCCCCG CTTTGGCGAA GGATGCAAAC 
AGCAGCACTG AAGCAAGGGT CAACGCACGG TTGGTCGTAA GCGAAGCTAC CGTGGCACTG 
CACATCGTAC AGCGCGCACA ACACCTCTTC TCCCATCTTG TCCTATACGT TGCTTCTGTG 
CTGTACTCAC CGAGTGGTCC AGCGAGGGAA GGAAGGGGAA CGAACGGTTC CCGTGGTGGA 
GAGCAACCTG ACGAGGTTTC TGCGGGGGAG TTGGACGCCT ACGTGGATTC TCAGCGGCGG 
CGATACGCAC TGCAGGGGCT TCTTGCGCTT CGTTCAAAGC TTACTTCCCA GGAGTCAAAC 
ACAGAGGGAG ATAACGAGAC CTCCGGAACA GGACGCGCTA AGTCTGTTAG GATGAGTGGG 
AGTCTCGCGC GGGAAGCGCT TTCAGCGTCG CAACGCGGCG GGTCAATTGC CTCGGCAACA 
GATGATAGTG AGAAAAAGGA TGGGGATTCC AAATGTGGGT CACTGGATGC CTCTTCTTCC 
ATGGTGGGAT TGAACCAAGA CGGTGAGCGA CGGTCTCGAC ACCGAACACT ATATCTCTGC 
GAAGTGATGC GGCAGGTTTG CGGGATGCAG ATCTTGGATC CTCTTCCATT GCAGTGGGGA 
TTCTCTTCCT TCTCTTCAAA TGAAAAGATT GCGAAGTGCA GCGGGGTTAG CGGTGATGAG 
ACAGCGGTTT TTAGCAAGGT GGGACACCAA CTGCGAGCTG TACAGGAGCT GGCGCTCCAG 
TTGCTGTTGC ATGTGTCTGG TGCCGGGCTG CTAAACGACC CGCGCACGAT TTCACTTCAA 
CAGCACCTTG CTCGCCAGCA GGGGAAAATG CTTCAGGATA TGTTGCTCTC ATCTGCGCAG 
GAGGGAAGTA ACTTAACTGT TTCTGGGGGT GCTGCTGTTA ACTTGTTTGA CGCACGCAAG 
TGGGGGGAAG CGGTTCGTCA GGCGACGGAG TCTGCACTCC TGAAGACTGT TGGAGCGGCA 
TTGAAGAGGG CAGATAGAGT TTGCGGCGCT ATACTGAAGC ATATAAGTGA GGGGAAAGGA 
TATAAAAGCA CCGTTGTCGC CTCCGCCGAA GAGCAGCCAC CCCAACAGCA GAAACAAGAG 
GGACCGCCCA GCGTGACAAC ATTTGCCCAG AAAGTCCTTC AGCGGTCCAT CTTCTTCAGC 
GAAAGTACAT CCTTTGGGCT GCTTCACACC GACCAAATGA CGTGCATGGT CCCGTGCTCC 
TCTTTTGGGG GTGGCGATGA AAATAAACGA AATGTGGCTT CAGCGGAGAA GGGCGAGAAT 
TTGAATGCCG CGTTGGCTCG GGGTATTATG CAGTGCACTG CAATAAAATC GAAAACAACC 
CCAAAGGAGG GAAGATTGCC GGCCAATCTT AGCTCTAAGG CGGGCTGTCG GGCATCCGGT 
GGGAACGTAA ATAAAGACAA GGGTGGATGG GTGATGCAAA CCGTGGAGAC AGATGCAGGA 
AGTGATGATA ATTTGTTCTC CGTTGATGAA GGAGAGGAGG AAAGGACAGT GGCTATGGAG 
AAAGCAAGTG ACAAATCAGA AAAGGGCGTC ACCGATGCTG TCGATGATGA TTCCTCGGCT 
GCTCTGAAGT TCGGCTCGCT GTATTCCATG TTGCAACGTA TCTTTGAGGT GGAGCGAACC 
TGGTCTGACT CGCACCCCAC ACTCTCTGTT CTCGTAGGGG AGTGGTTTAC AAATATCCTG 
CAACAGGTGC GTAAGCTGTT AAAGCACCAC ACGGGGCAAC TTCAGAAGTG GGAGGAGCTG 
AATGTGGAAG GCAACTGCTT TGAGGCGAAG GTGACGGTGA TGGCAGCACT CAACCGCATT 
TCCATGCTGG TGGAGGTGCT GTTGCAGGTG CTGGAAGTTG CAGCCCCACG AAATGTAGCG 
AGCACACTCC CCATGGAACT ACGTGAGGCC ATTGTAACAT GTCATACGCC GTGGGTGAAA 
TTGTTACGCA GCGAGTGGGA GGATGGTGTG CGTCATGCCT ACCGCCGCGC TTTCGCGCCC 
GAGCATCTCT TCGCGAGATC AAAGACGCCG CTGAACTCCT CTGCTACAAC CCTCGATCCC 
AACAAGAGGA CATCTTGTAT CAACGGACAA CAGCTCATGC TCGAGTACGC AACTTGCTGG 
AGCTGCAGCA CACCTGATGG GGTACAATGT GAGTCACAGA AAGTTACTAA GCACCCGACA 
CATGTCACCC CGCATGTGAA TGAACTTATT TTCCAGGTAC AGCATATGTT GTTCAAGAAT 
GGTGTCAACA AGCGTCTACG TGCCACGGTG CTGCCGATGG TCATAAATGA GTTACTTGAC 
GGAACCTCGT CAGCACTGCT TAACGCGGTT CTTCCGTGGA TAAGAGAGAT AGCGGCGGTA 
AATAGAAGCG GAAATGCCGG TAAGAGTGGC GATAGTGCTG GTGATAACGA CGAACTGCTG 
TTTCAAGTTT ATTTTGATTC ATTATACATT TCAGGTTTAT TCTCCATCGA CGGCAACAGT 
CGGGCACCGA CGCCGTCCAT TACCGCCGTT CTCCGCACAA TTGAAGGTTG GGTAAACCCC 
ACTTCTTGGT TAGTCATTTC ACCGCTACTT CTGCAAGCAA GCGACCGCTT GCTTCGCGTG 
ACGGCTCTCT CTTTTGGCTC ATGGAACCCC AAAGCTCTGG AGGAAGTTTG GACTGCAAAG 
GGAACTGGTG ACAGCCGGAC ATCCCTGAAA GGGGAGCGGA CTGTTGCCGG CGACATTAGC 
ATCACAAAAG AGAAGCCCCG TTTTCCTCTA CTGCCGTTGA TATTCACACC GTCGAAGGTA 
TTGGGATCAG ATATCGTGGA TGGTGGTGTA ACCAAGACGG CCGTGTTTGC TTTTAACAAA 
AGTAGTAGCG TCGCTGTGGG CGTGAGAGGC GATAGTGGTA TTATTGGTGG GTTGAGCGCG 
ACATCCCTTC AAAGGGGCAC TGGAGCACTG GTTGGTAGTG CACTTGGGAG TCCCTCCAAG 
CCGACATCGT CGAACCGTTT CGATGGCAAA GTGAAGGGGT GGATGGGCTC ATTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_951540.1
CDS	1..4200
Translation	MSSEEAIQEVRRIFTNNSVDQARDYLSRITPGIQESDERLKQLVGQSYRGLVAACDQVVVVEQACRKLLRLQKQMQEEKKQQQLENQYSQSRLTMPQSPRGVSSPVLRTISPHGGKSPRAKNVGEGKTQTSRHMGHSSSASPPVSEVVRSPLPHEATATLKGVCEETPRGGREGADDRQRDVLIYTQQCAALEGLLGARKLYDAAALLLQIRRERKQPHEKLAVESYEHRLKRAVRGLLREDAEQAAQSALSVANANRNDSGGGDLSLTSSTNMFTSSGGVEFMGRATGWKSDLSRALAHMTEVHKALGVLSHATVNDALLLLIDTIHDMIKTDLDHVVAALKGTSFTQAAAVDTNTDDGRSLRPLRRMEWLRAALELPPYLRSEGTSAPRVPALAKDANSSTEARVNARLVVSEATVALHIVQRAQHLFSHLVLYVASVLYSPSGPAREGRGTNGSRGGEQPDEVSAGELDAYVDSQRRRYALQGLLALRSKLTSQESNTEGDNETSGTGRAKSVRMSGSLAREALSASQRGGSIASATDDSEKKDGDSKCGSLDASSSMVGLNQDGERRSRHRTLYLCEVMRQVCGMQILDPLPLQWGFSSFSSNEKIAKCSGVSGDETAVFSKVGHQLRAVQELALQLLLHVSGAGLLNDPRTISLQQHLARQQGKMLQDMLLSSAQEGSNLTVSGGAAVNLFDARKWGEAVRQATESALLKTVGAALKRADRVCGAILKHISEGKGYKSTVVASAEEQPPQQQKQEGPPSVTTFAQKVLQRSIFFSESTSFGLLHTDQMTCMVPCSSFGGGDENKRNVASAEKGENLNAALARGIMQCTAIKSKTTPKEGRLPANLSSKAGCRASGGNVNKDKGGWVMQTVETDAGSDDNLFSVDEGEEERTVAMEKASDKSEKGVTDAVDDDSSAALKFGSLYSMLQRIFEVERTWSDSHPTLSVLVGEWFTNILQQVRKLLKHHTGQLQKWEELNVEGNCFEAKVTVMAALNRISMLVEVLLQVLEVAAPRNVASTLPMELREAIVTCHTPWVKLLRSEWEDGVRHAYRRAFAPEHLFARSKTPLNSSATTLDPNKRTSCINGQQLMLEYATCWSCSTPDGVQCESQKVTKHPTHVTPHVNELIFQVQHMLFKNGVNKRLRATVLPMVINELLDGTSSALLNAVLPWIREIAAVNRSGNAGKSGDSAGDNDELLFQVYFDSLYISGLFSIDGNSRAPTPSITAVLRTIEGWVNPTSWLVISPLLLQASDRLLRVTALSFGSWNPKALEEVWTAKGTGDSRTSLKGERTVAGDISITKEKPRFPLLPLIFTPSKVLGSDIVDGGVTKTAVFAFNKSSSVAVGVRGDSGIIGGLSATSLQRGTGALVGSALGSPSKPTSSNRFDGKVKGWMGSLW

Target ORF information:

RefSeq Version	XM_946447.1
Organism	Trypanosoma brucei brucei TREU927
Definition	Trypanosoma brucei brucei TREU927 hypothetical protein, conserved (Tb927.2.2370), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_946447.1

ORF Insert Sequence:

ATGTCATCGG AGGAAGCCAT TCAGGAAGTG CGACGCATTT TCACCAACAA TAGCGTGGAT 
CAGGCCCGCG ACTACCTGAG CCGTATAACA CCAGGCATTC AAGAAAGTGA TGAGAGACTC 
AAACAACTTG TCGGCCAAAG CTACCGGGGC CTCGTTGCCG CTTGTGACCA GGTGGTCGTC 
GTAGAACAGG CATGCAGGAA GTTGCTGCGT CTGCAAAAGC AAATGCAGGA GGAGAAAAAA 
CAGCAACAGT TGGAGAATCA ATATTCACAG TCGCGTCTGA CAATGCCGCA GAGCCCAAGG 
GGGGTCTCGT CACCCGTTTT GCGGACCATA TCCCCCCATG GAGGTAAAAG TCCGCGCGCG 
AAGAATGTGG GAGAAGGGAA AACTCAAACG AGTCGACATA TGGGACACTC AAGCAGTGCT 
TCGCCACCGG TTTCCGAGGT GGTGAGGTCC CCGTTGCCTC ATGAGGCAAC CGCGACTTTG 
AAGGGTGTGT GTGAAGAAAC GCCAAGAGGG GGGCGTGAGG GGGCAGATGA TAGACAACGA 
GACGTGTTAA TTTACACTCA GCAGTGCGCG GCTCTGGAGG GATTGTTGGG TGCCCGTAAG 
CTTTATGACG CCGCTGCGCT GCTCTTGCAG ATAAGAAGGG AACGGAAACA GCCTCATGAA 
AAGCTGGCTG TTGAGTCCTA TGAACATCGT CTCAAACGTG CAGTGCGTGG GCTGCTTCGG 
GAAGATGCAG AACAGGCCGC ACAAAGCGCT TTGAGTGTGG CTAACGCCAA TCGAAATGAT 
AGCGGTGGTG GGGACTTATC CTTGACATCG TCCACAAACA TGTTTACATC ATCAGGTGGT 
GTTGAATTCA TGGGTAGGGC AACAGGTTGG AAAAGTGATT TATCACGAGC ACTGGCTCAC 
ATGACGGAGG TCCACAAAGC CTTAGGAGTG CTTTCGCACG CAACAGTGAA TGACGCACTG 
TTACTCCTTA TCGATACGAT TCACGATATG ATTAAGACTG ACCTTGACCA CGTCGTGGCT 
GCACTGAAGG GAACGTCATT TACGCAGGCA GCGGCTGTAG ACACTAACAC TGACGATGGA 
AGAAGTTTAC GCCCACTTCG CAGAATGGAG TGGCTGCGGG CAGCCCTGGA GCTTCCGCCT 
TACTTAAGAA GCGAGGGAAC AAGTGCCCCA AGGGTCCCCG CTTTGGCGAA GGATGCAAAC 
AGCAGCACTG AAGCAAGGGT CAACGCACGG TTGGTCGTAA GCGAAGCTAC CGTGGCACTG 
CACATCGTAC AGCGCGCACA ACACCTCTTC TCCCATCTTG TCCTATACGT TGCTTCTGTG 
CTGTACTCAC CGAGTGGTCC AGCGAGGGAA GGAAGGGGAA CGAACGGTTC CCGTGGTGGA 
GAGCAACCTG ACGAGGTTTC TGCGGGGGAG TTGGACGCCT ACGTGGATTC TCAGCGGCGG 
CGATACGCAC TGCAGGGGCT TCTTGCGCTT CGTTCAAAGC TTACTTCCCA GGAGTCAAAC 
ACAGAGGGAG ATAACGAGAC CTCCGGAACA GGACGCGCTA AGTCTGTTAG GATGAGTGGG 
AGTCTCGCGC GGGAAGCGCT TTCAGCGTCG CAACGCGGCG GGTCAATTGC CTCGGCAACA 
GATGATAGTG AGAAAAAGGA TGGGGATTCC AAATGTGGGT CACTGGATGC CTCTTCTTCC 
ATGGTGGGAT TGAACCAAGA CGGTGAGCGA CGGTCTCGAC ACCGAACACT ATATCTCTGC 
GAAGTGATGC GGCAGGTTTG CGGGATGCAG ATCTTGGATC CTCTTCCATT GCAGTGGGGA 
TTCTCTTCCT TCTCTTCAAA TGAAAAGATT GCGAAGTGCA GCGGGGTTAG CGGTGATGAG 
ACAGCGGTTT TTAGCAAGGT GGGACACCAA CTGCGAGCTG TACAGGAGCT GGCGCTCCAG 
TTGCTGTTGC ATGTGTCTGG TGCCGGGCTG CTAAACGACC CGCGCACGAT TTCACTTCAA 
CAGCACCTTG CTCGCCAGCA GGGGAAAATG CTTCAGGATA TGTTGCTCTC ATCTGCGCAG 
GAGGGAAGTA ACTTAACTGT TTCTGGGGGT GCTGCTGTTA ACTTGTTTGA CGCACGCAAG 
TGGGGGGAAG CGGTTCGTCA GGCGACGGAG TCTGCACTCC TGAAGACTGT TGGAGCGGCA 
TTGAAGAGGG CAGATAGAGT TTGCGGCGCT ATACTGAAGC ATATAAGTGA GGGGAAAGGA 
TATAAAAGCA CCGTTGTCGC CTCCGCCGAA GAGCAGCCAC CCCAACAGCA GAAACAAGAG 
GGACCGCCCA GCGTGACAAC ATTTGCCCAG AAAGTCCTTC AGCGGTCCAT CTTCTTCAGC 
GAAAGTACAT CCTTTGGGCT GCTTCACACC GACCAAATGA CGTGCATGGT CCCGTGCTCC 
TCTTTTGGGG GTGGCGATGA AAATAAACGA AATGTGGCTT CAGCGGAGAA GGGCGAGAAT 
TTGAATGCCG CGTTGGCTCG GGGTATTATG CAGTGCACTG CAATAAAATC GAAAACAACC 
CCAAAGGAGG GAAGATTGCC GGCCAATCTT AGCTCTAAGG CGGGCTGTCG GGCATCCGGT 
GGGAACGTAA ATAAAGACAA GGGTGGATGG GTGATGCAAA CCGTGGAGAC AGATGCAGGA 
AGTGATGATA ATTTGTTCTC CGTTGATGAA GGAGAGGAGG AAAGGACAGT GGCTATGGAG 
AAAGCAAGTG ACAAATCAGA AAAGGGCGTC ACCGATGCTG TCGATGATGA TTCCTCGGCT 
GCTCTGAAGT TCGGCTCGCT GTATTCCATG TTGCAACGTA TCTTTGAGGT GGAGCGAACC 
TGGTCTGACT CGCACCCCAC ACTCTCTGTT CTCGTAGGGG AGTGGTTTAC AAATATCCTG 
CAACAGGTGC GTAAGCTGTT AAAGCACCAC ACGGGGCAAC TTCAGAAGTG GGAGGAGCTG 
AATGTGGAAG GCAACTGCTT TGAGGCGAAG GTGACGGTGA TGGCAGCACT CAACCGCATT 
TCCATGCTGG TGGAGGTGCT GTTGCAGGTG CTGGAAGTTG CAGCCCCACG AAATGTAGCG 
AGCACACTCC CCATGGAACT ACGTGAGGCC ATTGTAACAT GTCATACGCC GTGGGTGAAA 
TTGTTACGCA GCGAGTGGGA GGATGGTGTG CGTCATGCCT ACCGCCGCGC TTTCGCGCCC 
GAGCATCTCT TCGCGAGATC AAAGACGCCG CTGAACTCCT CTGCTACAAC CCTCGATCCC 
AACAAGAGGA CATCTTGTAT CAACGGACAA CAGCTCATGC TCGAGTACGC AACTTGCTGG 
AGCTGCAGCA CACCTGATGG GGTACAATGT GAGTCACAGA AAGTTACTAA GCACCCGACA 
CATGTCACCC CGCATGTGAA TGAACTTATT TTCCAGGTAC AGCATATGTT GTTCAAGAAT 
GGTGTCAACA AGCGTCTACG TGCCACGGTG CTGCCGATGG TCATAAATGA GTTACTTGAC 
GGAACCTCGT CAGCACTGCT TAACGCGGTT CTTCCGTGGA TAAGAGAGAT AGCGGCGGTA 
AATAGAAGCG GAAATGCCGG TAAGAGTGGC GATAGTGCTG GTGATAACGA CGAACTGCTG 
TTTCAAGTTT ATTTTGATTC ATTATACATT TCAGGTTTAT TCTCCATCGA CGGCAACAGT 
CGGGCACCGA CGCCGTCCAT TACCGCCGTT CTCCGCACAA TTGAAGGTTG GGTAAACCCC 
ACTTCTTGGT TAGTCATTTC ACCGCTACTT CTGCAAGCAA GCGACCGCTT GCTTCGCGTG 
ACGGCTCTCT CTTTTGGCTC ATGGAACCCC AAAGCTCTGG AGGAAGTTTG GACTGCAAAG 
GGAACTGGTG ACAGCCGGAC ATCCCTGAAA GGGGAGCGGA CTGTTGCCGG CGACATTAGC 
ATCACAAAAG AGAAGCCCCG TTTTCCTCTA CTGCCGTTGA TATTCACACC GTCGAAGGTA 
TTGGGATCAG ATATCGTGGA TGGTGGTGTA ACCAAGACGG CCGTGTTTGC TTTTAACAAA 
AGTAGTAGCG TCGCTGTGGG CGTGAGAGGC GATAGTGGTA TTATTGGTGG GTTGAGCGCG 
ACATCCCTTC AAAGGGGCAC TGGAGCACTG GTTGGTAGTG CACTTGGGAG TCCCTCCAAG 
CCGACATCGT CGAACCGTTT CGATGGCAAA GTGAAGGGGT GGATGGGCTC ATTGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

The genome of the African trypanosome Trypanosoma brucei.
Science (New York, N.Y.)309(5733)416-22(2005 Jul)
Berriman M,Ghedin E,Hertz-Fowler C,Blandin G,Renauld H,Bartholomeu DC,Lennard NJ,Caler E,Hamlin NE,Haas B,Böhme U,Hannick L,Aslett MA,Shallom J,Marcello L,Hou L,Wickstead B,Alsmark UC,Arrowsmith C,Atkin RJ,Barron AJ,Bringaud F,Brooks K,Carrington M,Cherevach I,Chillingworth TJ,Churcher C,Clark LN,Corton CH,Cronin A,Davies RM,Doggett J,Djikeng A,Feldblyum T,Field MC,Fraser A,Goodhead I,Hance Z,Harper D,Harris BR,Hauser H,Hostetler J,Ivens A,Jagels K,Johnson D,Johnson J,Jones K,Kerhornou AX,Koo H,Larke N,Landfear S,Larkin C,Leech V,Line A,Lord A,Macleod A,Mooney PJ,Moule S,Martin DM,Morgan GW,Mungall K,Norbertczak H,Ormond D,Pai G,Peacock CS,Peterson J,Quail MA,Rabbinowitsch E,Rajandream MA,Reitter C,Salzberg SL,Sanders M,Schobel S,Sharp S,Simmonds M,Simpson AJ,Tallon L,Turner CM,Tait A,Tivey AR,Van Aken S,Walker D,Wanless D,Wang S,White B,White O,Whitehead S,Woodward J,Wortman J,Adams MD,Embley TM,Gull K,Ullu E,Barry JD,Fairlamb AH,Opperdoes F,Barrell BG,Donelson JE,Hall N,Fraser CM,Melville SE,El-Sayed NM

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Tb927.2.2370 cDNA ORF clone, Trypanosoma brucei brucei TREU927

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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