TGME49_228395 cDNA ORF clone, Toxoplasma gondii ME49

Gene
Clones
gRNAs

Summary

Gene Symbol	TGME49_228395
Entrez Gene ID	29769084
Full Name	hypothetical protein
Synonyms	TGME49_028395
Gene Type	protein-coding
Organism	Toxoplasma gondii ME49

mRNA and Protein(s)

mRNA	Protein	Name
XM_018780208.1	XP_018636117.1	hypothetical protein

Pathways from BioSystems
Gene Ontology

The following TGME49_228395 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGME49_228395 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi10829	XM_018780208.1 Latest version!	Toxoplasma gondii ME49 hypothetical protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGME49_228395 ( XM_018780208.1 ) cDNA ORF clone, Toxoplasma gondii ME49 -> XP_018636117.1 Toxoplasma gondii ME49 hypothetical protein partial mRNA.

Price & Availability↑

CloneID	OTi10829
Clone ID Related Accession (Same CDS sequence)	XM_018780208.1
Accession Version	XM_018780208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476806400000
Organism	Toxoplasma gondii ME49
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_031478). COMPLETENESS: incomplete on both ends.

ATGAGCGACA CATCAGGCAA GGCCGGAGCA CACTTTCGTT CTTCGGAAGC CCTTTCTTCG 
GAGGAGACGA AGCATGCGGG ACGCGCACTC TTCAGCGAAG TGGGCAGTCC TACGCCTGGT 
GACGCCTGCA ACGGTGGCAT GACTTCCTCT TCGGTGCTCG CATGGCCCGA TGGAAATTTT 
TGCGCGCGTA AGGATGACAA GGCTTTCGAG GGAGCCCCCA CGCCGTGGGC ACAGATCAGA 
GCTGCGGAAC GTTTTCGTGT CGCGAGACCA CTCATAGGCG ATGGGGTGTC CGTGGACGCT 
CAACCAGGCT TTGCCTTCAC GTCCGACGAC GATGACTCGG TTCTCATTCA CCAGGCGGAT 
TCAATGGACG CCGTGGGTCA ACAAGACACG CCCGTTTCGT CCCGCCATCT CGAGCGTGTG 
CTAAGTTTTG AGACTTTCAC AGTCCCGCAT CGCTTACGGA AACGGGCGCT GCCCATTGCA 
GCTGCTGGCA TGCTCGCAGC GGAGGAAAGG AGCCACAGTA CAGAGAGCTC GACAGACCAA 
CCGCAGGGCC TCCATAAAGG CGGGAGACGA CGGTTTCAAA GTGAATGCTA TGCAGCGGAC 
GGTTTCGCTC TGCATCGCCG CCGGCGTTCT GCAGGTCCGC ATTCTGCAGA CAGCAAGGCT 
CAGTGCGACG CTTTGCCCAG TGCTTTTTCA GGTGCACACA CGCCGTTTGT GGAGCCAAAT 
GTTTCTTATC AGCGGGACCC AGTGCCAGTA GCTGGCCGGC TCGAGGTCAA ACCGGAGCGG 
CTAACAAGAC TGGCATTGCT CCACCGACAA ATATGCGACA GAAGTTTACC ATTTCCCTTT 
ACGAATCGAC GGGGGAGAGT GCGGTTCCCG AATGAGACTC TTCCGCCCCG AGTGATTCGG 
GAGCAGCTGC ATGGCCTGCG TCGTCTCTTT CGCCGCATTA CACATGTGAT TAGGCATACC 
ACATTCTTGT GGCATGAGGA AGACGATGAC TCGCTGTTGG GTGACAACAT GTCGGATCCG 
GGGGACCGGA AAGACAGTTA CTGCGGCCAC GGGAGGAAGG CGCCGTGTCC GGATGCATCG 
GACAAGCGCG CAGAGGCGCC GAGTTCGGAG CGGCAGTGCA GTGTTTCTAA GGAGAGCTGT 
CCGGGCGGAA GGCGCCGAAG CTGCAGCGCA GGCGAATTAG AACTTTTCCT TAAAGCGTCG 
AAGGATCAGG CAACTCAGCA TGGCGAAACA GTGAGAGCCG GAGCTGCACG CTTGGTGGCC 
ACAGAGACGG AAGAAACGTT CGAAGACGAC GGGGTCATCA CAGAGGACGT CCCTGATCAA 
AAAACGATGA CGACCGCCGG CCCCACTGTC TGGGCCCATA GCGAAGTCCT GAGCGACGAG 
AAGGACCCAA CACAGCGCAC TCGTGACATG CCGTATTTGA CGCAGAAATC CTGCAGTTTT 
GGAGCGGAAC GCAGCCGGAG GCGACACGGG GAAGTTGATC TTTGCTGCCT TCTTCGCCGA 
CTCATAACTG TGCTGCAGCA CGGAGGGCCT GTCGCGCCCA AGGACGTTGC TCTTCGCCCG 
AAGGAACGAA AAATACGCAG TTGGCTAGTC TCGACGGAGT CTACCAGCTC CCTGCCTTCT 
CCAAGTCTAT ACGGCCATCA GTACTCTGGA GAAAACGCAT GGCAGAGACA GACGGAACGA 
GATCACAGGG AAGGAAGCAC AACTTTTTCG TGTCCAGTCT CTGCTCTCGA TCTTCTCCCT 
TTGTCGTTTA TCAGAACGCT GCGCCTTCTT CTTGTCCATC CCGAGGCGCA GTGCCGCGTT 
CTCGCCATTG AAGCATGCAC GCACTTACTT GCTTCTGCAG TGCAGCATCG GCGCTTCGCG 
TCTCTCCAGA TCCCAAATAT AATATCGAAA GCTCTGCTTC GCGAGCCTGA GGTTTTGCTA 
CATCCGTGTT TTGAACATCG ACACAAAAGT GGAATGTGCC CCTCACAAAC AGGAAGGCTC 
GCGACGGACC GAAGATGGCC CTCCGAACCG CATGCGCCCC TTTCCTATTC CGGAATCGCC 
TCGTATGGTG CGGCCGAGTG GAACGAGCCG TTGGCAGCCA CAGTGTCAGC GACATGCTCC 
AGCGCTCTGT TCTCTGAGCG AGAAGCAGCG CTCATGTTCA TCCGGGTTTG GACCGCTTTC 
TGCCGCGCCG CACCCCGAGC TACGGATACG CGTGGGAGAA AGCTTGCCGC GTCTAGAAGG 
GAACCTTGTG GTGCATCCTA CTGCCGGTGT GTCGATCTGG ACCTTTTCAC TCGCCCTCTT 
CTCCCGGATG TTTTCGTTTA TACGGTCACC GCCGAGGCTG CTGACACCGA AGATGGTGGA 
ACCGCCAATA TGGCCGGAGC AGCACAGCCG AACCCCGCTC ATTTTCGGGC TGCAAGCACA 
GGCGGAAAAA CGTCCAGCAG AAATATGCCA CATGCACACG TTGCACCAAA CAGAGCAACT 
ACAGCACGTC AAGCGGAGCA AGCGGTCAGG GGGATCGAGG GAAAGGCCCA CGCTACACTC 
GGGGCCGGAG GCGGCCAGCG ACCTGACGAT AGCGGAGGCA TACACCAGGG AGGGCGAAAT 
CGGAGCTTGT TGACGCGTGA GATACCCTCA GGTCTCCAGT GGAGGGTAGC GTGCTTGGAG 
ACGATTCTGG AGTTCGGTTT TCTGTGTCCG TGCATTCTGG TGGAGACTAG AACCGCCGGT 
GTCCTTGTGG ATGCACTAAC GGACGTTGAG GCGCAGGCCT ACAACCCCAG GTTAGGCCCC 
TCCATCTGCA TTTTCCTTAT TGGCCTTCTT TCCCATCGGT ACCCGGAAAG ACTCCCGGAA 
GAGCCTTCAG TGTCTCCGCC TAACGACAGC ATGAGCACCT TGGCCTGCCA CACAATTTCA 
CCAGTCGTCG GGGGGCGAGG TACGCGCTGG TCATCCTTGA GCGAACAGTT TTCGTCGTGC 
CGGTACACAA GGAGTGTGCA CGGGAAAGGC TGGCTCAAAC AGGATGCCGA CACGGCGGTG 
AAAACGGTCC CGGAAGATCG GTGCGTTTGT GGTCGCCCGC TCTCTTCTTC GTCCCGCATC 
CAGCTCGAAC AAACGTGTAT CACAGAGCTG TCGCGTCTTT GTGGCGTCGC CGTCGAGATT 
GTCCAGGCGT GCGCGTCTGC AGAAACGATT GCCGCTGCTG CTGGGACAGG GAAAGACGGC 
GCCGGACGTG GAAGAGTTCG GGGCGGGCCT GTCCACCATC AAGCGGCACC GTTGCCCGTG 
TTTCAGTTTC GCAGGGCACC TGCTGGGGAT GCCGACGCGC CGACTCGAGG GCCGGGGGCT 
ACGGCGACAG GCAAATCACC AGCAGCAGGT TTTCCCGGTG CTCGCGGTCC GCCGAAAAAC 
CAGCTTTTGG CTGGTGTGAA CGTACCCAGT GGCGTTATCT CCGCTCTCTG GTTGTATCGG 
CGGTGGATCT TAGTCTCGTC CGCACTGCTC TGTCTGTCCC AGTCTTTCTC GGCGTTGGTG 
GCGCTCTGGC GCCTACCCAC GGGGCTGCCG TCGCTGGTGA GGCTTCTCGC CAGCCCCATC 
GATCCTTGCA CCATGTGTTG GATTCTGCGC CTTTTCCACG CCATGCTGTC TTATGCAGTG 
GGCTTTGAGG GCCGCCGTAT GACGCTTGCC GAGTGCGACA GCCTAAGCAA CCGGCAGATC 
GACAAGCCGG CGAGGGAGCA CCCGTCTGCA CGCGCAAGTG GATGCCGAAG AGAAGGTGCG 
TCCTCCCACG ACGATCGATA CTGGTCCTCT TCCTTGGTGC GAGAGCCTGC TGGGATGGAC 
GTTATCATGC GTAGACGGTT TCATTCCGGA AAAGAGGACG AGAGACCGTT CTCTTGCCCC 
TGGGCTTCTG ATAACGGACA CAGGCCGCCG TTTTCCGGCA CCTCAAGTCC CAGACGAAGA 
CCTAGCGTGT CTGGCCTCTA CTCAAGTCCG TTTGGGTCGC GCGACCTAAC CAGGGGCACA 
TCGGCACTCT CCGTTGGAAG CCCGTTCGGG TCGGGGTCTC CTGCGTCTCT CAGTCAAGAC 
TACGGCAGGC CCAATTGGTC GGAAGCGTCA GATCCCGCGA GAAGTGCATA TGCAGACATG 
GCCTTTAGCT TCGCCTCCAG TTCCGGGGAG GCTCCCGCGG ACAGGAGCGG GGGCGGGAGA 
AACCAATCCC CCGGAAAAGC GCCGTATCAT TTCATCTCCT CGACAAACCA GCCAGTGACA 
AGAGATATTG TCCACACTCA TTCGCTTCTG GTTGCGCGCC TCATGCTCCA CGCCCAACTC 
CACGTCGTCC TTGCGGGCCT CTGCTCACTT CGCGATGACC ACCATAGCCG TACCGCAGAT 
ACACCTTGCT TGATTCCTGT CATTCCCTCC GATGTGCAGT TCGTCCCGGA GACTCTGAAG 
ATCGACGTTC ACGGGGTGAC GGAGATGTCA TCTTCCTTCG CACAGTGCCT CCTCTGCCTG 
GAACGGGGAC CGGCCCCAAG GGGCATCGCG GGGACGTGCA GCAACAGGGC AGACCTTCTT 
TCTGCGACCC AACGTCAGCG CTGCCTCAAA GACTCCGTGC TCGACACGCG GCTGCCCGGA 
TCCACGCTAC ACGATTTAAT CCCTTTTGCC GCCATCGTAC GGACTGAAGC AGCGCACTTA 
CTGTATGCCC TCGTTCACAC AGTTGCAGCC TACTTTCCGC TGCACCTGCT TCGCCACAAA 
TTGGATGCCG AAACTGTCTT CCGGGAGTTA CGGCCTCCTC CAGCTGGGGC GCCAAGTCGG 
TCCGCCTGGG TTGATCGGGA CCCGTCCACG AGAACATCGA AGCAGAGCAG ACAAGCTGGC 
CAGTCTTTGG ATGCGCAGTC GCAGGAATTT TATCGGTGTC CTGTCGATGC CGATTCGGCA 
TGGCCTCAGT CCAGGTGGCA GCAGATGGGC CAAATGCGAG GTTATCAAGG CCCTCGTGAT 
AGACTCTCAC AAAACAGATG TGGGGGTCAG GGGGGGAACG AGCTGCATCG CGCCACAAAT 
TCCGCGGACG AGCTGCGGTT GCGAAAACCT TTCGGCGAGA ACTACCAGGC GAATACGAGG 
TCGGAAACCG CGCCGCGGTC GGTCCACAAC GTTGCGGGTT TGGACCACGT GTCCCTAGTC 
ACGCTGTCAG AGTCTACGAC ACGAGCAGAA CAGGCTTCGT CCTCTGTGTG GCATGGCCCC 
CGAAATTACC CCCGACTCCT GGCCTCTTGC TTCAAGAGAG GTTCTGATTC GACGAGTCTG 
AGCGTAATTT CGACGCTCTC TCTCCTGCAC TTTGTAGCAG CCCCACCCGT GCGCTGTGGA 
CTCCGCTCGC TACTTGCTGG TGGCAAGCTG GTATGCAAAG GCGAAGACGA TGGCATCTGC 
GGCTCCCTGC GCCTTCTTTT GCGGTGCGGG GACCCACGAA GAGCTCCGCA GGATCAGACG 
TATGACGGCC TGCTATCGCA GTATGATCGC GAACCAACGC GTCGGGATTT GGTGACGTCT 
TGGACGTTTG ATAGTGAGGA CGAGGCCGGG GAGGCGGATG TGGGAGAGGC GAGCTTCTTC 
GTTGCTCCGA ACCCAGAGGG AGACTTGCAA GATTCGCTGG CGGGCCTCAG ATATTCCGCG 
AGACTCTCAG CGTTGACGGA GAACGGCGCA GGGGCGCCTG GCACTGCTGA GGCAGGTTGT 
GGCGCCTCGA CCTACTCCGG GCCTGCCTTC GTGCAGCGTC CGTACCCGCA GCAGAATTCT 
GAGGGAGGCA CTCGTCAGGT GTGTGGCAAA TACAGTGTGA CGACGCGAAT GACGCGCTGC 
CAACAGAAAC GCATCCGTCC GTCTCCGTCA TCCGAGACAG GCATTTTGTA TGATCGGCGT 
GGAGTTGCGG TGCACGGCAT CTCGACATCT TCCCGGATTC AGGAAGAGAT TCTTAGGCGT 
CTGGTGGTCA TGCACGAAGA GGAAGAAGGA CAAACAAAGC ACGTGGGAAG CTGCCCGACA 
CTCAAGATGC TCAGCGACGT CGTCCCGTAC ATCTTCGGAG ACAGTGCTCG CATTGGAGGG 
AGCTGTCAAA TGGGAAGTGT CAAGAACATC CATCCAGGTC CTGGCAATGA AATTCAGTCT 
TCGGCGGGTG AAGGCGCTTC CTGCGTCGCC AATGTAAGGG GCCGGCTGCA GGACCCCCAG 
ACGTGCACTC CGAGCGTTTC CTGGATGTCG GCCTTAATCG AGCTCATGAA CGCTACTGGA 
GTTTTGCAGC ACCCCGTTCA GAATGCTGCC CTCTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018636117.1
CDS	1..6219
Translation	MSDTSGKAGAHFRSSEALSSEETKHAGRALFSEVGSPTPGDACNGGMTSSSVLAWPDGNFCARKDDKAFEGAPTPWAQIRAAERFRVARPLIGDGVSVDAQPGFAFTSDDDDSVLIHQADSMDAVGQQDTPVSSRHLERVLSFETFTVPHRLRKRALPIAAAGMLAAEERSHSTESSTDQPQGLHKGGRRRFQSECYAADGFALHRRRRSAGPHSADSKAQCDALPSAFSGAHTPFVEPNVSYQRDPVPVAGRLEVKPERLTRLALLHRQICDRSLPFPFTNRRGRVRFPNETLPPRVIREQLHGLRRLFRRITHVIRHTTFLWHEEDDDSLLGDNMSDPGDRKDSYCGHGRKAPCPDASDKRAEAPSSERQCSVSKESCPGGRRRSCSAGELELFLKASKDQATQHGETVRAGAARLVATETEETFEDDGVITEDVPDQKTMTTAGPTVWAHSEVLSDEKDPTQRTRDMPYLTQKSCSFGAERSRRRHGEVDLCCLLRRLITVLQHGGPVAPKDVALRPKERKIRSWLVSTESTSSLPSPSLYGHQYSGENAWQRQTERDHREGSTTFSCPVSALDLLPLSFIRTLRLLLVHPEAQCRVLAIEACTHLLASAVQHRRFASLQIPNIISKALLREPEVLLHPCFEHRHKSGMCPSQTGRLATDRRWPSEPHAPLSYSGIASYGAAEWNEPLAATVSATCSSALFSEREAALMFIRVWTAFCRAAPRATDTRGRKLAASRREPCGASYCRCVDLDLFTRPLLPDVFVYTVTAEAADTEDGGTANMAGAAQPNPAHFRAASTGGKTSSRNMPHAHVAPNRATTARQAEQAVRGIEGKAHATLGAGGGQRPDDSGGIHQGGRNRSLLTREIPSGLQWRVACLETILEFGFLCPCILVETRTAGVLVDALTDVEAQAYNPRLGPSICIFLIGLLSHRYPERLPEEPSVSPPNDSMSTLACHTISPVVGGRGTRWSSLSEQFSSCRYTRSVHGKGWLKQDADTAVKTVPEDRCVCGRPLSSSSRIQLEQTCITELSRLCGVAVEIVQACASAETIAAAAGTGKDGAGRGRVRGGPVHHQAAPLPVFQFRRAPAGDADAPTRGPGATATGKSPAAGFPGARGPPKNQLLAGVNVPSGVISALWLYRRWILVSSALLCLSQSFSALVALWRLPTGLPSLVRLLASPIDPCTMCWILRLFHAMLSYAVGFEGRRMTLAECDSLSNRQIDKPAREHPSARASGCRREGASSHDDRYWSSSLVREPAGMDVIMRRRFHSGKEDERPFSCPWASDNGHRPPFSGTSSPRRRPSVSGLYSSPFGSRDLTRGTSALSVGSPFGSGSPASLSQDYGRPNWSEASDPARSAYADMAFSFASSSGEAPADRSGGGRNQSPGKAPYHFISSTNQPVTRDIVHTHSLLVARLMLHAQLHVVLAGLCSLRDDHHSRTADTPCLIPVIPSDVQFVPETLKIDVHGVTEMSSSFAQCLLCLERGPAPRGIAGTCSNRADLLSATQRQRCLKDSVLDTRLPGSTLHDLIPFAAIVRTEAAHLLYALVHTVAAYFPLHLLRHKLDAETVFRELRPPPAGAPSRSAWVDRDPSTRTSKQSRQAGQSLDAQSQEFYRCPVDADSAWPQSRWQQMGQMRGYQGPRDRLSQNRCGGQGGNELHRATNSADELRLRKPFGENYQANTRSETAPRSVHNVAGLDHVSLVTLSESTTRAEQASSSVWHGPRNYPRLLASCFKRGSDSTSLSVISTLSLLHFVAAPPVRCGLRSLLAGGKLVCKGEDDGICGSLRLLLRCGDPRRAPQDQTYDGLLSQYDREPTRRDLVTSWTFDSEDEAGEADVGEASFFVAPNPEGDLQDSLAGLRYSARLSALTENGAGAPGTAEAGCGASTYSGPAFVQRPYPQQNSEGGTRQVCGKYSVTTRMTRCQQKRIRPSPSSETGILYDRRGVAVHGISTSSRIQEEILRRLVVMHEEEEGQTKHVGSCPTLKMLSDVVPYIFGDSARIGGSCQMGSVKNIHPGPGNEIQSSAGEGASCVANVRGRLQDPQTCTPSVSWMSALIELMNATGVLQHPVQNAALW

Target ORF information:

RefSeq Version	XM_018780208.1
Organism	Toxoplasma gondii ME49
Definition	Toxoplasma gondii ME49 hypothetical protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018780208.1

ORF Insert Sequence:

ATGAGCGACA CATCAGGCAA GGCCGGAGCA CACTTTCGTT CTTCGGAAGC CCTTTCTTCG 
GAGGAGACGA AGCATGCGGG ACGCGCACTC TTCAGCGAAG TGGGCAGTCC TACGCCTGGT 
GACGCCTGCA ACGGTGGCAT GACTTCCTCT TCGGTGCTCG CATGGCCCGA TGGAAATTTT 
TGCGCGCGTA AGGATGACAA GGCTTTCGAG GGAGCCCCCA CGCCGTGGGC ACAGATCAGA 
GCTGCGGAAC GTTTTCGTGT CGCGAGACCA CTCATAGGCG ATGGGGTGTC CGTGGACGCT 
CAACCAGGCT TTGCCTTCAC GTCCGACGAC GATGACTCGG TTCTCATTCA CCAGGCGGAT 
TCAATGGACG CCGTGGGTCA ACAAGACACG CCCGTTTCGT CCCGCCATCT CGAGCGTGTG 
CTAAGTTTTG AGACTTTCAC AGTCCCGCAT CGCTTACGGA AACGGGCGCT GCCCATTGCA 
GCTGCTGGCA TGCTCGCAGC GGAGGAAAGG AGCCACAGTA CAGAGAGCTC GACAGACCAA 
CCGCAGGGCC TCCATAAAGG CGGGAGACGA CGGTTTCAAA GTGAATGCTA TGCAGCGGAC 
GGTTTCGCTC TGCATCGCCG CCGGCGTTCT GCAGGTCCGC ATTCTGCAGA CAGCAAGGCT 
CAGTGCGACG CTTTGCCCAG TGCTTTTTCA GGTGCACACA CGCCGTTTGT GGAGCCAAAT 
GTTTCTTATC AGCGGGACCC AGTGCCAGTA GCTGGCCGGC TCGAGGTCAA ACCGGAGCGG 
CTAACAAGAC TGGCATTGCT CCACCGACAA ATATGCGACA GAAGTTTACC ATTTCCCTTT 
ACGAATCGAC GGGGGAGAGT GCGGTTCCCG AATGAGACTC TTCCGCCCCG AGTGATTCGG 
GAGCAGCTGC ATGGCCTGCG TCGTCTCTTT CGCCGCATTA CACATGTGAT TAGGCATACC 
ACATTCTTGT GGCATGAGGA AGACGATGAC TCGCTGTTGG GTGACAACAT GTCGGATCCG 
GGGGACCGGA AAGACAGTTA CTGCGGCCAC GGGAGGAAGG CGCCGTGTCC GGATGCATCG 
GACAAGCGCG CAGAGGCGCC GAGTTCGGAG CGGCAGTGCA GTGTTTCTAA GGAGAGCTGT 
CCGGGCGGAA GGCGCCGAAG CTGCAGCGCA GGCGAATTAG AACTTTTCCT TAAAGCGTCG 
AAGGATCAGG CAACTCAGCA TGGCGAAACA GTGAGAGCCG GAGCTGCACG CTTGGTGGCC 
ACAGAGACGG AAGAAACGTT CGAAGACGAC GGGGTCATCA CAGAGGACGT CCCTGATCAA 
AAAACGATGA CGACCGCCGG CCCCACTGTC TGGGCCCATA GCGAAGTCCT GAGCGACGAG 
AAGGACCCAA CACAGCGCAC TCGTGACATG CCGTATTTGA CGCAGAAATC CTGCAGTTTT 
GGAGCGGAAC GCAGCCGGAG GCGACACGGG GAAGTTGATC TTTGCTGCCT TCTTCGCCGA 
CTCATAACTG TGCTGCAGCA CGGAGGGCCT GTCGCGCCCA AGGACGTTGC TCTTCGCCCG 
AAGGAACGAA AAATACGCAG TTGGCTAGTC TCGACGGAGT CTACCAGCTC CCTGCCTTCT 
CCAAGTCTAT ACGGCCATCA GTACTCTGGA GAAAACGCAT GGCAGAGACA GACGGAACGA 
GATCACAGGG AAGGAAGCAC AACTTTTTCG TGTCCAGTCT CTGCTCTCGA TCTTCTCCCT 
TTGTCGTTTA TCAGAACGCT GCGCCTTCTT CTTGTCCATC CCGAGGCGCA GTGCCGCGTT 
CTCGCCATTG AAGCATGCAC GCACTTACTT GCTTCTGCAG TGCAGCATCG GCGCTTCGCG 
TCTCTCCAGA TCCCAAATAT AATATCGAAA GCTCTGCTTC GCGAGCCTGA GGTTTTGCTA 
CATCCGTGTT TTGAACATCG ACACAAAAGT GGAATGTGCC CCTCACAAAC AGGAAGGCTC 
GCGACGGACC GAAGATGGCC CTCCGAACCG CATGCGCCCC TTTCCTATTC CGGAATCGCC 
TCGTATGGTG CGGCCGAGTG GAACGAGCCG TTGGCAGCCA CAGTGTCAGC GACATGCTCC 
AGCGCTCTGT TCTCTGAGCG AGAAGCAGCG CTCATGTTCA TCCGGGTTTG GACCGCTTTC 
TGCCGCGCCG CACCCCGAGC TACGGATACG CGTGGGAGAA AGCTTGCCGC GTCTAGAAGG 
GAACCTTGTG GTGCATCCTA CTGCCGGTGT GTCGATCTGG ACCTTTTCAC TCGCCCTCTT 
CTCCCGGATG TTTTCGTTTA TACGGTCACC GCCGAGGCTG CTGACACCGA AGATGGTGGA 
ACCGCCAATA TGGCCGGAGC AGCACAGCCG AACCCCGCTC ATTTTCGGGC TGCAAGCACA 
GGCGGAAAAA CGTCCAGCAG AAATATGCCA CATGCACACG TTGCACCAAA CAGAGCAACT 
ACAGCACGTC AAGCGGAGCA AGCGGTCAGG GGGATCGAGG GAAAGGCCCA CGCTACACTC 
GGGGCCGGAG GCGGCCAGCG ACCTGACGAT AGCGGAGGCA TACACCAGGG AGGGCGAAAT 
CGGAGCTTGT TGACGCGTGA GATACCCTCA GGTCTCCAGT GGAGGGTAGC GTGCTTGGAG 
ACGATTCTGG AGTTCGGTTT TCTGTGTCCG TGCATTCTGG TGGAGACTAG AACCGCCGGT 
GTCCTTGTGG ATGCACTAAC GGACGTTGAG GCGCAGGCCT ACAACCCCAG GTTAGGCCCC 
TCCATCTGCA TTTTCCTTAT TGGCCTTCTT TCCCATCGGT ACCCGGAAAG ACTCCCGGAA 
GAGCCTTCAG TGTCTCCGCC TAACGACAGC ATGAGCACCT TGGCCTGCCA CACAATTTCA 
CCAGTCGTCG GGGGGCGAGG TACGCGCTGG TCATCCTTGA GCGAACAGTT TTCGTCGTGC 
CGGTACACAA GGAGTGTGCA CGGGAAAGGC TGGCTCAAAC AGGATGCCGA CACGGCGGTG 
AAAACGGTCC CGGAAGATCG GTGCGTTTGT GGTCGCCCGC TCTCTTCTTC GTCCCGCATC 
CAGCTCGAAC AAACGTGTAT CACAGAGCTG TCGCGTCTTT GTGGCGTCGC CGTCGAGATT 
GTCCAGGCGT GCGCGTCTGC AGAAACGATT GCCGCTGCTG CTGGGACAGG GAAAGACGGC 
GCCGGACGTG GAAGAGTTCG GGGCGGGCCT GTCCACCATC AAGCGGCACC GTTGCCCGTG 
TTTCAGTTTC GCAGGGCACC TGCTGGGGAT GCCGACGCGC CGACTCGAGG GCCGGGGGCT 
ACGGCGACAG GCAAATCACC AGCAGCAGGT TTTCCCGGTG CTCGCGGTCC GCCGAAAAAC 
CAGCTTTTGG CTGGTGTGAA CGTACCCAGT GGCGTTATCT CCGCTCTCTG GTTGTATCGG 
CGGTGGATCT TAGTCTCGTC CGCACTGCTC TGTCTGTCCC AGTCTTTCTC GGCGTTGGTG 
GCGCTCTGGC GCCTACCCAC GGGGCTGCCG TCGCTGGTGA GGCTTCTCGC CAGCCCCATC 
GATCCTTGCA CCATGTGTTG GATTCTGCGC CTTTTCCACG CCATGCTGTC TTATGCAGTG 
GGCTTTGAGG GCCGCCGTAT GACGCTTGCC GAGTGCGACA GCCTAAGCAA CCGGCAGATC 
GACAAGCCGG CGAGGGAGCA CCCGTCTGCA CGCGCAAGTG GATGCCGAAG AGAAGGTGCG 
TCCTCCCACG ACGATCGATA CTGGTCCTCT TCCTTGGTGC GAGAGCCTGC TGGGATGGAC 
GTTATCATGC GTAGACGGTT TCATTCCGGA AAAGAGGACG AGAGACCGTT CTCTTGCCCC 
TGGGCTTCTG ATAACGGACA CAGGCCGCCG TTTTCCGGCA CCTCAAGTCC CAGACGAAGA 
CCTAGCGTGT CTGGCCTCTA CTCAAGTCCG TTTGGGTCGC GCGACCTAAC CAGGGGCACA 
TCGGCACTCT CCGTTGGAAG CCCGTTCGGG TCGGGGTCTC CTGCGTCTCT CAGTCAAGAC 
TACGGCAGGC CCAATTGGTC GGAAGCGTCA GATCCCGCGA GAAGTGCATA TGCAGACATG 
GCCTTTAGCT TCGCCTCCAG TTCCGGGGAG GCTCCCGCGG ACAGGAGCGG GGGCGGGAGA 
AACCAATCCC CCGGAAAAGC GCCGTATCAT TTCATCTCCT CGACAAACCA GCCAGTGACA 
AGAGATATTG TCCACACTCA TTCGCTTCTG GTTGCGCGCC TCATGCTCCA CGCCCAACTC 
CACGTCGTCC TTGCGGGCCT CTGCTCACTT CGCGATGACC ACCATAGCCG TACCGCAGAT 
ACACCTTGCT TGATTCCTGT CATTCCCTCC GATGTGCAGT TCGTCCCGGA GACTCTGAAG 
ATCGACGTTC ACGGGGTGAC GGAGATGTCA TCTTCCTTCG CACAGTGCCT CCTCTGCCTG 
GAACGGGGAC CGGCCCCAAG GGGCATCGCG GGGACGTGCA GCAACAGGGC AGACCTTCTT 
TCTGCGACCC AACGTCAGCG CTGCCTCAAA GACTCCGTGC TCGACACGCG GCTGCCCGGA 
TCCACGCTAC ACGATTTAAT CCCTTTTGCC GCCATCGTAC GGACTGAAGC AGCGCACTTA 
CTGTATGCCC TCGTTCACAC AGTTGCAGCC TACTTTCCGC TGCACCTGCT TCGCCACAAA 
TTGGATGCCG AAACTGTCTT CCGGGAGTTA CGGCCTCCTC CAGCTGGGGC GCCAAGTCGG 
TCCGCCTGGG TTGATCGGGA CCCGTCCACG AGAACATCGA AGCAGAGCAG ACAAGCTGGC 
CAGTCTTTGG ATGCGCAGTC GCAGGAATTT TATCGGTGTC CTGTCGATGC CGATTCGGCA 
TGGCCTCAGT CCAGGTGGCA GCAGATGGGC CAAATGCGAG GTTATCAAGG CCCTCGTGAT 
AGACTCTCAC AAAACAGATG TGGGGGTCAG GGGGGGAACG AGCTGCATCG CGCCACAAAT 
TCCGCGGACG AGCTGCGGTT GCGAAAACCT TTCGGCGAGA ACTACCAGGC GAATACGAGG 
TCGGAAACCG CGCCGCGGTC GGTCCACAAC GTTGCGGGTT TGGACCACGT GTCCCTAGTC 
ACGCTGTCAG AGTCTACGAC ACGAGCAGAA CAGGCTTCGT CCTCTGTGTG GCATGGCCCC 
CGAAATTACC CCCGACTCCT GGCCTCTTGC TTCAAGAGAG GTTCTGATTC GACGAGTCTG 
AGCGTAATTT CGACGCTCTC TCTCCTGCAC TTTGTAGCAG CCCCACCCGT GCGCTGTGGA 
CTCCGCTCGC TACTTGCTGG TGGCAAGCTG GTATGCAAAG GCGAAGACGA TGGCATCTGC 
GGCTCCCTGC GCCTTCTTTT GCGGTGCGGG GACCCACGAA GAGCTCCGCA GGATCAGACG 
TATGACGGCC TGCTATCGCA GTATGATCGC GAACCAACGC GTCGGGATTT GGTGACGTCT 
TGGACGTTTG ATAGTGAGGA CGAGGCCGGG GAGGCGGATG TGGGAGAGGC GAGCTTCTTC 
GTTGCTCCGA ACCCAGAGGG AGACTTGCAA GATTCGCTGG CGGGCCTCAG ATATTCCGCG 
AGACTCTCAG CGTTGACGGA GAACGGCGCA GGGGCGCCTG GCACTGCTGA GGCAGGTTGT 
GGCGCCTCGA CCTACTCCGG GCCTGCCTTC GTGCAGCGTC CGTACCCGCA GCAGAATTCT 
GAGGGAGGCA CTCGTCAGGT GTGTGGCAAA TACAGTGTGA CGACGCGAAT GACGCGCTGC 
CAACAGAAAC GCATCCGTCC GTCTCCGTCA TCCGAGACAG GCATTTTGTA TGATCGGCGT 
GGAGTTGCGG TGCACGGCAT CTCGACATCT TCCCGGATTC AGGAAGAGAT TCTTAGGCGT 
CTGGTGGTCA TGCACGAAGA GGAAGAAGGA CAAACAAAGC ACGTGGGAAG CTGCCCGACA 
CTCAAGATGC TCAGCGACGT CGTCCCGTAC ATCTTCGGAG ACAGTGCTCG CATTGGAGGG 
AGCTGTCAAA TGGGAAGTGT CAAGAACATC CATCCAGGTC CTGGCAATGA AATTCAGTCT 
TCGGCGGGTG AAGGCGCTTC CTGCGTCGCC AATGTAAGGG GCCGGCTGCA GGACCCCCAG 
ACGTGCACTC CGAGCGTTTC CTGGATGTCG GCCTTAATCG AGCTCATGAA CGCTACTGGA 
GTTTTGCAGC ACCCCGTTCA GAATGCTGCC CTCTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TGME49_228395 cDNA ORF clone, Toxoplasma gondii ME49

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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