ORF » Species Summary » Thalassiosira pseudonana CCMP1335 » ITS3 cDNA ORF clone

ITS3 cDNA ORF clone, Thalassiosira pseudonana CCMP1335

The following ITS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OTf05451 XM_002288885.1
Latest version!		 Thalassiosira pseudonana CCMP1335 probable isoleucine-trna synthetase (ITS3), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ITS3 ( XM_002288885.1 ) cDNA ORF clone, Thalassiosira pseudonana CCMP1335 -> XP_002288921.1 Thalassiosira pseudonana CCMP1335 probable isoleucine-trna synthetase (ITS3), partial mRNA.
    Price & Availability↑
    CloneID OTf05451
    Clone ID Related Accession (Same CDS sequence) XM_002288885.1
    Accession Version XM_002288885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3312bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Thalassiosira pseudonana CCMP1335
    Product probable isoleucine-trna synthetase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_012066). COMPLETENESS: incomplete on the 5' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGGCTCGC TCAACCTGAA CAGCGCCCCA AAAGGAACCG CCAAACGCGA TGCCCTCGTC 
    TCCTCCGAGA TTGCCGTCCA AGCTCTCTGG GAACGCGAAA AGGCATTTGA ATCCAACCCC 
    ACCTACAAAG AGGACGGGAC TTCCGAGGAT AAATTCATCG TTACGTTTCC GTATCCTTAT 
    AGTAATGGTC ATTTGCATTT GGGACATGCG TTTAGTTTGA CCAAGGCTGT GTTTCGGGCG 
    CAGTTTGAGA GGAATCGGGG GAAGAACTCT CTGTTTCCTT TTGCGTTTCA TTGTACGGGG 
    ATGCCTATTC AGGCGGCCGC CAACAAACTC AAGTCCGAAA TCACCCAATA CGGCATCCCA 
    CCCAAATTCC CCGAGGAGGA CCCCGCCGTC CGCGCCCAAA TGGAAGCTGA TCTCGCCGCC 
    GCCGCCAAAG CCAAGGCCGA AAAAGCCGCA GCCTCCGGCT CCAAAGCCAA GGGGGGCAAG 
    ACCAAACTCG TCCAAAAGAC GGGGACTGGC ATTGTTCGTC AGTGGAACAT TTTGAAACGA 
    ATGGTTCCTG AGGAGGAGAT TCCGGAGTTT GCCGATCCGA TTCATTGGTT GAAGTACTTT 
    CCTCCCATTG GGGTGGAGCA TATGAAGCGG TTTGGAAGTG GAGTTGATTG GAGGAGGTCG 
    TTTATTACTA CAGCCGTGAA TGGGTATTAT GATGCATTTA TCCGATGGCA GTTTAATGTG 
    TTGAGGGAGA AGGGGAAGGT ATTGTTTGGG AAGAGGAACA ATGTTTATTC CATCTTGGAT 
    GGGCAGGTTT GTGCGGATCA TGATCGTAGT GAGGGAGAGG GTGTGGGACC TCAGGAGTAT 
    GTGTTGATCA AGTTGAAGGT ATTGGAGCCT GATCATGGAC AGGCGAGGCA TGGAAAGATG 
    GAGGCGTTGT TGAAAAGGGT AGAGGACGAG GGGAAGAAGG GTGTTTTTAT GGTTCCTGCC 
    ACGTTACGTC CCGAGACCAT GTATGGACAG ACGAACTGCT TCGTTCTCCC CACTGGAGAG 
    TATGGGGCAT ATTACATTGA TGCTACAGAC GAAGTGTTCA TCATGAGTGC TAGATCGGCT 
    CGTGGTCTTT CGTGTCAGGC GTATGATGCT GCCAATGACG TTTACTTTAC CAAGGAGTTT 
    GGAAAGATTG AGTGCTTGGA GACGTTTACA GGTGATGAGC TTTTGGGATT GCCTCTCAAG 
    GCACCCAATG CCACCTATGA GAAGGTGTAT ACGCTTCCAC TTCTTACCAT TAGTATGGGC 
    AAGGGCACGG GTGTTGTGAC TTCAGTCCCT TCCGATGCTC CTGACGATTA TGTGGCGTTG 
    AAGGCTTTGC AAGACAAGCC TGACTTTGCT GCCAAATACG ACATCACTCC TGATATGGTG 
    GATCCGTTTG AGGTGGTTCC CATCATTTCC ATTGAGGGTT ACGGCGATGC GTCTGCAGTA 
    TTCATGTGCG AGAAGCTTGG AATTACTAGT CCAAACGACA AGGCAAAGCT TGCTCAGGCA 
    AAGGATGAGA CGTATCTCAA AGGATTCACA ATGGGTGTCA TGAATGTGGG TCCGCATGCT 
    GGAAAGAAGG TGTCGGAGGC AAAGCCCATC ATTAAGGATG AAATGATTAC AGCTGGACAA 
    GCTCATCTAT ATTTTGAGCC AGAGTCCAAA GTTGTTTCTC GTACCAATGA TGAGTGTGTT 
    GTTGCTTCGA CTGATCAGTG GTACCTCGCC TATGGAGAGG ACTCTTGGTG TTCGGCTGTG 
    AAGAACCACG TGCTTGATAG TGAGAAGTTC AATGCCTACG ACGCCACAGC GTTGGAGAAG 
    TACGATGCCA CTCTTGATTG GTTGAAAGAG TGGGCGTGCA CTCGTCAGTT CGGCCTTGGA 
    ACGCAGTTGC CTTGGGATCA GCACTGGGTG ATTGAAAGTC TTTCGGATAG TACCATCTAC 
    ATGTCGTACT ATACGATTGC TCACTACTTG CAGGGAGAGA ACAACCTTAA TGGAGACGAG 
    TCAAAGAGCC CTGAGAATAT TAAGGTGGAG GATTTGACGG ATGATGTATT CAACTTTATC 
    TACCGCAAGG GATTCTCCGT TCCAGAGAAC TGCTGCATTT CTGCAGAGAC TCTTGAGAAG 
    ATGAGGGCTG AATTCCGTTA CTGGTATCCT ATGGATCTTC GTGTGTCTGC CAAAGATCTC 
    ATCCCCAACC ACTTGACAAT GGCACTTTAC AATCATGCTG CAATGTGGGA TGACGAACCA 
    GAGCTTTGGC CCCGTGGGTA CTACACCAAT GGTCATATTA TGGTAGATGC GGAGAAGATG 
    AGTAAATCCA AGGGCAACTT CTTGATGATG CTTGAAACCA TTGAGAATTA CTCTGCCGAT 
    GCCACTCGTT TTGCATGCGC TGATGCTGGT GACACCCTTG ACGATGCCAA CTTTAGCAGG 
    GATACTGCCA ACACTGCCAT TGTGTCTCTC TCCAATGAGG CTGCTTGGAT TAAGGAGGTT 
    CTCTTGGACA CTGACAAATC AACCTTGAGA TCTGGCGATG AACTCAACTT CATGGACAAG 
    GTGTTTGAGA ATGAGACCAA CCGTCTTATC AATGAAACAG AGAGGTGCTT TGAGACAATG 
    CAGTTCCGTG AAGGACTTCA GAAAGGATGG TTTGAGATGA TGATTGCTAG GAATGAGTAC 
    CGAAGTTGGT GCCAAGATAG TGGCGTTCCT TTACACGAGG GACTTGTCAG AAAGTGGGCT 
    GAAAGCTTGA TTATTATCAT CTGCCCCATC TGCCCTCATT GGTCTGAAAC TCTTTGGAAG 
    GACCTCGGAA AGGAAGGTTT GGCTGTGCGA GCGTTGTGGC CTGTTGTCGG CGAAGAAGAC 
    AAGCTCCTCA CTCGTCAATC AAAATTTCTT CGTGATTCGT TGAAAAACTT CCGCGCTCAA 
    GCTGGTAAAG CAAAGAAGGG ATGGAAGATT GCCACCATCC TGGTTGCAGA GGATTATCCC 
    CAGTGGAAGG TGGATGCTCT TCTTTGGATG CAAAGCAAAT ACGACAAAGC CACTGGTTCT 
    TTCCCTGATA CTTTCATGAC AGATTTGAAG GATTGGTCCA CCACCAATGT GACTGATAAG 
    AAGCAGGTGA AGTTTACAAT GCAGTTCGTT TCCTTCACGA AGAAGGAGGT GGAAGACGTC 
    GGAGAGACGG GAATGGATAC GAAGTGCCCC TTTGATCAGC TTGCTATTCT GAAGGAGAGC 
    AAGGGTTATC TTCAATCTCA GCTTGGTATG GAAGAGATTG GCATTGGCAG CGTTGACGAT 
    CCTGAATTAG CTGTCCCAGA AAAGAATGCT CAAAATGTTT CGCCTGGAAA GCCGTACCTT 
    TGGATTCGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002288921.1
    CDS1..3312
    Translation

    Target ORF information:

    RefSeq Version XM_002288885.1
    Organism Thalassiosira pseudonana CCMP1335
    Definition Thalassiosira pseudonana CCMP1335 probable isoleucine-trna synthetase (ITS3), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_002288885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGGCTCGC TCAACCTGAA CAGCGCCCCA AAAGGAACCG CCAAACGCGA TGCCCTCGTC 
    TCCTCCGAGA TTGCCGTCCA AGCTCTCTGG GAACGCGAAA AGGCATTTGA ATCCAACCCC 
    ACCTACAAAG AGGACGGGAC TTCCGAGGAT AAATTCATCG TTACGTTTCC GTATCCTTAT 
    AGTAATGGTC ATTTGCATTT GGGACATGCG TTTAGTTTGA CCAAGGCTGT GTTTCGGGCG 
    CAGTTTGAGA GGAATCGGGG GAAGAACTCT CTGTTTCCTT TTGCGTTTCA TTGTACGGGG 
    ATGCCTATTC AGGCGGCCGC CAACAAACTC AAGTCCGAAA TCACCCAATA CGGCATCCCA 
    CCCAAATTCC CCGAGGAGGA CCCCGCCGTC CGCGCCCAAA TGGAAGCTGA TCTCGCCGCC 
    GCCGCCAAAG CCAAGGCCGA AAAAGCCGCA GCCTCCGGCT CCAAAGCCAA GGGGGGCAAG 
    ACCAAACTCG TCCAAAAGAC GGGGACTGGC ATTGTTCGTC AGTGGAACAT TTTGAAACGA 
    ATGGTTCCTG AGGAGGAGAT TCCGGAGTTT GCCGATCCGA TTCATTGGTT GAAGTACTTT 
    CCTCCCATTG GGGTGGAGCA TATGAAGCGG TTTGGAAGTG GAGTTGATTG GAGGAGGTCG 
    TTTATTACTA CAGCCGTGAA TGGGTATTAT GATGCATTTA TCCGATGGCA GTTTAATGTG 
    TTGAGGGAGA AGGGGAAGGT ATTGTTTGGG AAGAGGAACA ATGTTTATTC CATCTTGGAT 
    GGGCAGGTTT GTGCGGATCA TGATCGTAGT GAGGGAGAGG GTGTGGGACC TCAGGAGTAT 
    GTGTTGATCA AGTTGAAGGT ATTGGAGCCT GATCATGGAC AGGCGAGGCA TGGAAAGATG 
    GAGGCGTTGT TGAAAAGGGT AGAGGACGAG GGGAAGAAGG GTGTTTTTAT GGTTCCTGCC 
    ACGTTACGTC CCGAGACCAT GTATGGACAG ACGAACTGCT TCGTTCTCCC CACTGGAGAG 
    TATGGGGCAT ATTACATTGA TGCTACAGAC GAAGTGTTCA TCATGAGTGC TAGATCGGCT 
    CGTGGTCTTT CGTGTCAGGC GTATGATGCT GCCAATGACG TTTACTTTAC CAAGGAGTTT 
    GGAAAGATTG AGTGCTTGGA GACGTTTACA GGTGATGAGC TTTTGGGATT GCCTCTCAAG 
    GCACCCAATG CCACCTATGA GAAGGTGTAT ACGCTTCCAC TTCTTACCAT TAGTATGGGC 
    AAGGGCACGG GTGTTGTGAC TTCAGTCCCT TCCGATGCTC CTGACGATTA TGTGGCGTTG 
    AAGGCTTTGC AAGACAAGCC TGACTTTGCT GCCAAATACG ACATCACTCC TGATATGGTG 
    GATCCGTTTG AGGTGGTTCC CATCATTTCC ATTGAGGGTT ACGGCGATGC GTCTGCAGTA 
    TTCATGTGCG AGAAGCTTGG AATTACTAGT CCAAACGACA AGGCAAAGCT TGCTCAGGCA 
    AAGGATGAGA CGTATCTCAA AGGATTCACA ATGGGTGTCA TGAATGTGGG TCCGCATGCT 
    GGAAAGAAGG TGTCGGAGGC AAAGCCCATC ATTAAGGATG AAATGATTAC AGCTGGACAA 
    GCTCATCTAT ATTTTGAGCC AGAGTCCAAA GTTGTTTCTC GTACCAATGA TGAGTGTGTT 
    GTTGCTTCGA CTGATCAGTG GTACCTCGCC TATGGAGAGG ACTCTTGGTG TTCGGCTGTG 
    AAGAACCACG TGCTTGATAG TGAGAAGTTC AATGCCTACG ACGCCACAGC GTTGGAGAAG 
    TACGATGCCA CTCTTGATTG GTTGAAAGAG TGGGCGTGCA CTCGTCAGTT CGGCCTTGGA 
    ACGCAGTTGC CTTGGGATCA GCACTGGGTG ATTGAAAGTC TTTCGGATAG TACCATCTAC 
    ATGTCGTACT ATACGATTGC TCACTACTTG CAGGGAGAGA ACAACCTTAA TGGAGACGAG 
    TCAAAGAGCC CTGAGAATAT TAAGGTGGAG GATTTGACGG ATGATGTATT CAACTTTATC 
    TACCGCAAGG GATTCTCCGT TCCAGAGAAC TGCTGCATTT CTGCAGAGAC TCTTGAGAAG 
    ATGAGGGCTG AATTCCGTTA CTGGTATCCT ATGGATCTTC GTGTGTCTGC CAAAGATCTC 
    ATCCCCAACC ACTTGACAAT GGCACTTTAC AATCATGCTG CAATGTGGGA TGACGAACCA 
    GAGCTTTGGC CCCGTGGGTA CTACACCAAT GGTCATATTA TGGTAGATGC GGAGAAGATG 
    AGTAAATCCA AGGGCAACTT CTTGATGATG CTTGAAACCA TTGAGAATTA CTCTGCCGAT 
    GCCACTCGTT TTGCATGCGC TGATGCTGGT GACACCCTTG ACGATGCCAA CTTTAGCAGG 
    GATACTGCCA ACACTGCCAT TGTGTCTCTC TCCAATGAGG CTGCTTGGAT TAAGGAGGTT 
    CTCTTGGACA CTGACAAATC AACCTTGAGA TCTGGCGATG AACTCAACTT CATGGACAAG 
    GTGTTTGAGA ATGAGACCAA CCGTCTTATC AATGAAACAG AGAGGTGCTT TGAGACAATG 
    CAGTTCCGTG AAGGACTTCA GAAAGGATGG TTTGAGATGA TGATTGCTAG GAATGAGTAC 
    CGAAGTTGGT GCCAAGATAG TGGCGTTCCT TTACACGAGG GACTTGTCAG AAAGTGGGCT 
    GAAAGCTTGA TTATTATCAT CTGCCCCATC TGCCCTCATT GGTCTGAAAC TCTTTGGAAG 
    GACCTCGGAA AGGAAGGTTT GGCTGTGCGA GCGTTGTGGC CTGTTGTCGG CGAAGAAGAC 
    AAGCTCCTCA CTCGTCAATC AAAATTTCTT CGTGATTCGT TGAAAAACTT CCGCGCTCAA 
    GCTGGTAAAG CAAAGAAGGG ATGGAAGATT GCCACCATCC TGGTTGCAGA GGATTATCCC 
    CAGTGGAAGG TGGATGCTCT TCTTTGGATG CAAAGCAAAT ACGACAAAGC CACTGGTTCT 
    TTCCCTGATA CTTTCATGAC AGATTTGAAG GATTGGTCCA CCACCAATGT GACTGATAAG 
    AAGCAGGTGA AGTTTACAAT GCAGTTCGTT TCCTTCACGA AGAAGGAGGT GGAAGACGTC 
    GGAGAGACGG GAATGGATAC GAAGTGCCCC TTTGATCAGC TTGCTATTCT GAAGGAGAGC 
    AAGGGTTATC TTCAATCTCA GCTTGGTATG GAAGAGATTG GCATTGGCAG CGTTGACGAT 
    CCTGAATTAG CTGTCCCAGA AAAGAATGCT CAAAATGTTT CGCCTGGAAA GCCGTACCTT 
    TGGATTCGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The Phaeodactylum genome reveals the evolutionary history of diatom genomes.
    Nature456(7219)239-44(2008 Nov)
    Bowler C,Allen AE,Badger JH,Grimwood J,Jabbari K,Kuo A,Maheswari U,Martens C,Maumus F,Otillar RP,Rayko E,Salamov A,Vandepoele K,Beszteri B,Gruber A,Heijde M,Katinka M,Mock T,Valentin K,Verret F,Berges JA,Brownlee C,Cadoret JP,Chiovitti A,Choi CJ,Coesel S,De Martino A,Detter JC,Durkin C,Falciatore A,Fournet J,Haruta M,Huysman MJ,Jenkins BD,Jiroutova K,Jorgensen RE,Joubert Y,Kaplan A,Kröger N,Kroth PG,La Roche J,Lindquist E,Lommer M,Martin-Jézéquel V,Lopez PJ,Lucas S,Mangogna M,McGinnis K,Medlin LK,Montsant A,Oudot-Le Secq MP,Napoli C,Obornik M,Parker MS,Petit JL,Porcel BM,Poulsen N,Robison M,Rychlewski L,Rynearson TA,Schmutz J,Shapiro H,Siaut M,Stanley M,Sussman MR,Taylor AR,Vardi A,von Dassow P,Vyverman W,Willis A,Wyrwicz LS,Rokhsar DS,Weissenbach J,Armbrust EV,Green BR,Van de Peer Y,Grigoriev IV


    The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism.
    Science (New York, N.Y.)306(5693)79-86(2004 Oct)
    Armbrust EV,Berges JA,Bowler C,Green BR,Martinez D,Putnam NH,Zhou S,Allen AE,Apt KE,Bechner M,Brzezinski MA,Chaal BK,Chiovitti A,Davis AK,Demarest MS,Detter JC,Glavina T,Goodstein D,Hadi MZ,Hellsten U,Hildebrand M,Jenkins BD,Jurka J,Kapitonov VV,Kröger N,Lau WW,Lane TW,Larimer FW,Lippmeier JC,Lucas S,Medina M,Montsant A,Obornik M,Parker MS,Palenik B,Pazour GJ,Richardson PM,Rynearson TA,Saito MA,Schwartz DC,Thamatrakoln K,Valentin K,Vardi A,Wilkerson FP,Rokhsar DS


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