TTHERM_00227350 cDNA ORF clone, Tetrahymena thermophila SB210

Gene
Clones
gRNAs

Summary

Gene Symbol	TTHERM_00227350
Entrez Gene ID	7831274
Full Name	helicase carboxy-terminal domain protein
Gene Type	protein-coding
Organism	Tetrahymena thermophila SB210

mRNA and Protein(s)

mRNA	Protein	Name
XM_001014266.1	XP_001014266.1	helicase carboxy-terminal domain protein

Pathways from BioSystems
Gene Ontology

The following TTHERM_00227350 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTHERM_00227350 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTe45201	XM_001014266.1 Latest version!	Tetrahymena thermophila SB210 helicase carboxy-terminal domain protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTHERM_00227350 ( XM_001014266.1 ) cDNA ORF clone, Tetrahymena thermophila SB210 -> XP_001014266.1 Tetrahymena thermophila SB210 helicase carboxy-terminal domain protein partial mRNA.

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CloneID	OTe45201
Clone ID Related Accession (Same CDS sequence)	XM_001014266.1
Accession Version	XM_001014266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433692800000
Organism	Tetrahymena thermophila SB210
Product	helicase carboxy-terminal domain protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002476432). COMPLETENESS: incomplete on both ends.

ATGAATCAGC CAGAAATATC AAATAGAGAA AACAATTTAG ATGCCAAAAA ATTGATAGAA 
TTATTCTAGG AATACGAATA AGACTATAGC GAATTAAAAG ATAAGGATAT AATCTTATTA 
GCAGGACCTA CTGGAAGCGG AAAGAGTACT ACTGCTAACT ATCTTTTAGG TTATGAAATG 
TTTTTTGAAA AGATTGAAAT AGAGAAAGAA GTAGATGGTG AAATCTATTA AAGTGAAACT 
GAAGTTTTTA ATTCAAATGG AATATTTGAA ATTGGGCACT CTGGAAAATC ACATACTTCA 
ACACTGTAAA AAGCTCATTT ACAAGGTAAT TATTTTTTGT GCGATTCTCC TGGATTTTTT 
GATAATCGTG GTACTGAAAT CGAAATCGCT AATGCATATT CTTTAAAGTA ATTTATGTTG 
AATTGCAATA GTGTGAAAAT GGTTGTTTTG ATAAGCTTTT TTGAATTAGA GGCTCGAAGA 
GGAGCATTAT TTTAAGAGAT ATTTAAACTT GTAACTAGAA TTCTTTAAAA CCCAGAGTAA 
GAGTAATTTG AAAAGATAAT ATTTCTATTT ACTAAGGTTC CTAATGATAA AAGCTTTAAA 
ACTATTAATA GTAAGATTCT AGATTTTCTT GATATTATGA AAAACTAAGA AGAAGATAGG 
GAATCATTTA CAATGAAAAC CTTTTTGAAA GTTATTTTAA ATTAACTTAA AATCAATAGT 
AATTTAGTAA TTGTAAATCC TGCTGATGGA AATCATAGGT TATTACTTTA AAAGATCCTT 
AGCCATTAAT CTTTCAGTAA TCCTGCTGAA GAATTTTGCT TTAGTGTTTC AGAAAATGCA 
AATTAATAGA TAAAGTTATA CACAGAGGAG ACGAGCAAAA TTATATTAAA ATAATAATAA 
TATGGAAAGT ATATGGAAGT AAAGAATAGA ATGGTTGAAT TCAAGTAAAT AAGGGACTAT 
ACAGGGAATG AATTTTTGTT ATATTCATAT CAAGATTTAT CGAAAGAAAT TGTAAGAATC 
TTTGAAAAAT AAAAAAACTA GGCTTTTAAC ACTTTTAAGG AAGCTTCTGA ATAAAGCAAC 
ATATTTTCCT CCCAAAATAT TAAAGAAATA CTAGATATTA TTTCATATTT ATAAATGATT 
GATGAATTTA AAGCTGAAGT TTTTGAATAA GGCTAATTTA AAACTAGCGA AAATCAATTC 
AAATAAGAGA TTGAACAAGT TTGCTTTGAG ATAAGTAAAA GGATAACTGA AGATTTCCCT 
TAAAACATCG AAAATGTTAA GAATAATCTA TAAAAATTAA AAGATTTTTC AGATTAAACT 
TCTTTAGGTT TGTAAATATA TAAGTAAACC TAAAATGAAA TAAGGGATAA ATATTTGTTA 
GAAATAGAAG ACTTGAAATA ATTGTCTGAA AGATTTTACA TAAGGACATA ATAATTAGAG 
GTTAACTCAA TCAAAGATAG TGTAACCAAT ATAGCTTAGA AAATTGGCTT TATAAAATTA 
ATTGAAAAAT TCCTCAAAGC TTTTATCTTA AATGAAGGCG AAAATTTTTA CGAAACTATA 
GTTCAGTTAC ATAAGCAAAA AAATAATGGG CTGTTATCAA ATCTAAAGTT AATAATTGCC 
GATATTACTG AAGAGATTTA AAAATAACCC TCTATGGATG ACTCTGAGAT TGTTTTTAAT 
CCTTAATTAG AGTGCCTGCC AAATTTTGAA ATTAGCATAC TCAAAATATA AAACTAGAAT 
AGCAAATATT CTGAATATAT TCCAGACTGC GAGATTTTAT ATAATGAAGC TCTTTTACAT 
TTAAAAGAAT TTAATTAACA AGTATTTAAT TTTATCAAAG AAAATTCTTA AGAAGATTTT 
CATTTGCATA AACTATAAAA TATCTTAAAA ATATTAGACG AAATCAGAAA TCAAGATGAT 
TTGATTTAAG GTAAAACTGC TGACGAATAT AGATAAATCA AGGATGAAAT AAAACAAAAA 
ATTAATAAAA AGGCTCGTGA AGCTAAAAAA ATTTTAAATT AATATTTATA GGAAATTGAA 
GAAGAAGAAA ACAACTGTGA CGAAGAAGAA GATGAAGATG ATATTAATTC AATAAAATCA 
AATGATTTAG TAAAAAACTT TAAATTAAAG TAATAAAATG ACTTTAAGTA GTTGTTGAAG 
GCATACAATT TTCTTAGTGA TATGCATTGG ACAGATTAAT ATTTTTAAAC AAAATTTACA 
TAAACTAGCA TAAATAAACT AGAAGTAAAG TTAAATAAAT ATTTAGAGTA GTGCAAGTAG 
AATGTTTTTA ACTTGATCAA AAATGATAAA TTCTGTTAAA TAAAAAAAGT CTTTTTAAGA 
ATAAGTTGCA TTAGTGTTTT TAAAGATGTA GTTAAAAACT ATGAAGGACA ATATCAGATG 
TGTGTAGATT CAGTGAAGAA AAAAGTATCA GAGTTGAATG AACAAATAAA AAAATTTATT 
AGTTGTAAGA GAGGAGATAA TATATTTAAT AGTGAAGGCT GGGAAAAATT AAATTAATAT 
ATATTGCTTT TGTCTAAGAT TGAGACTTTA GACAAGATTG CTGATGTCAA GGGAGATTCT 
AATTCTTCAC TAATTGAAAT CAAGTGTTTA ATCTAGACAT ATTTTGAAGA AAATTTCCAA 
TTAATAAAGA ATTTATCAAG AGCTAAAGTT AACCCAAATG ACGAAGATTT CACAATTTAA 
TCTAATTTTG TTAAACAAGC GAAATTAATA AATATAGAAT ATCAATATAT TAAGTCGCTT 
TTAAAAAGAT TCTCTTTTGA ATAACTTATG GCTTTGGTTT AAAACGTTTA CGATGAGATG 
GTCACTGATT ACATTTCTAA GAAAGAATAT ATTGAGAATC TTTAAGAGAT TTAAGGGAGC 
TATGATAGAA TTAACAGATT TATCTAAAGC ATGAGTGAAT TTGATTTCTT AAAAGAGAAA 
TTTAAAAGTT TGCCTAGACC AAAAGAGACT ATATAGTAGG TATTTAAGAA CCTTGAAAAT 
TTGATAAAAA ATGAAGATTT TTAGAACCTA GATTCAGAAA TATACTTATT GAGCCAACTT 
AAAAGCGATA CTGATAAATA AGCTTTAGAT AAATCATTTT AAAAAATATA CGACAAGCTC 
TATAATGAAA TATAATAAGT AAAGAGTTAT TTTTATTTTA AATAAAATGG AGGTTTCTCT 
GAGAATATTA GAAAAAATCT GAAGATTGTA TCTGAAATAT TAAGAGTGCT CGAGAAATAT 
AAGAATCAGC TTTACTAATA ATCTCGTAAT GAATTAAATA TTTTTTTGAA TATCTTTAGG 
TAAAATTACA TAAAGTCTTA AAAGTAAATG ATTTTTAGTA AAATAAAACA ACTAAAATTC 
TAAGAAATGG AATAGTAATA CGAATAATTA ATGTAAACTT TTACTGGCTT GAGTGAATTT 
AAATACATTT TCTAATAAGA TGATTGGAAT ACTTTTAGCT CAGAAATTTA AAACATAAAA 
GAGGAAATTT CAAAGTCTAT TTTAAATCTT CATCATCTTT CGAAAGAGTT AATATATCTT 
TTAAATTTGC TTTAAGGACT AAATGAAGCT TAGAAGCACC CTTCAGATTT TTCAGAGACA 
TTTTAACAAG AATTACAAAA TTCCCAATAA GAAATTAAGT TAGTATTTAA TAATTATGAA 
ACGAGCATTA GAAATAAAAT AAAAAATAAG GATAGCTTTA TTAAAACTGA CTTAAAAGTT 
TTGAAAAATT TTGCTTAGAA AGCAGAAAAT ATTGAATAAT TGAATTATTG TGATTTTTAA 
GTTAAACTAT TTTAAATTGA AGAAGAAATA TATGAAGAAT CAAATAAGAA TTATTAAGAA 
ATTTAAAATT TATTGGTTAA AAAGGATTAT GACTAGGCTA AAAAACAGCT TCTACAAATT 
AAGTAGGCAA ATGAGCAGTT TTTCGATAGA ATAATAAAAA ATATGTAAAT TATGATATCC 
AAAGCTACAG ATTAAATTAA AGACCAATTC CCCTTTATAG AAGTTTCATG CTTCTAGAAT 
ACTTTTTTAA CTGCCTTAAA CAACCTGAAA ATAATAAAAC ACTTTGTAAA TTAAGATGAG 
TATCTTCAAC ATGAAACAAC AATTACTAAA CTTATTACTC ATATATTTAC ATAATCAAGG 
AAGTTCTTTG AGGAATGCGA TAAAATTCTG ATCTACAATG ATCAATCGTT TACATATTAA 
AATTTAATAA ATCTATTTAA ACATCACTCA TTTTTTGAAG TAATTCAAAG AGAGACGGTT 
TAAGGAGCAG AGTAAGCAAA GCCTTTATAA GATCTTTTTA AAAGTATTTT AGTTAAAGTA 
AAAGAAGGAC TATTAAAAGT AAAATAGGCT CAACAATTAA TTAAAGAGAA AATGTAAGTT 
GATCAAGCCT TAAATAAAAA TTGGAAAAAA TTCAATGCTC TTAATCAAAT CCTAAAGGAC 
TCACCTTAAT TGTTTGAATA ATCTGAAGAA CTTAAAAAAA TAAAAGAAAT TGGTCTTCAA 
ATTTTGAATG ACGATTAACT AGTAAAGGAA ATTTAAAAAA ATGTTGAAAG CAACGATTAA 
GCTTTTTAAA CTGCATTGGA AAATTGCAAT TTCTAAAGAG TTAAAGAGAT CATTTTTAGC 
ATAAGGAAAT ACTCTTAAAT AAATTAAGAA TTTAATACTG AATTCATCGA CATTAATTCC 
ATGCTAAAAA AAATTGAGTA AAAGGTTAAT CAAATAGTGC AAGAAGCACT AAATATAGTG 
AAAGCTAGTA TTATTTATGA CTAAAAAATG AATGATTTAA TGAATATGAT TTATAATGCA 
GATGAGCATT TGAAAAGCAT AGATTAATAT CCTTATGAAC CTTAAATCTA AATAGTTTTA 
AAAGAGCTTA CTAATAAATT AGATCAAGAA ATCTCTGATC TTCCTAAATA GAGCTTTATG 
TATTAATCAA ACCTAAAAGA ATTAAGCCAA TTTTTAATTA GCTTACAAAA AATTTCTGAT 
TAAGTGAGTA AATTCACTTA AATTGTTGAT AAAAAGTTAT TTGAAGCTTT TGAAATCATA 
ATGTAAACTT TGGGAGAAGA TGTAATATTT GAATTAGGTA ATATTCTAAA GCAAGATGCT 
TCTGGGGCTC AGATTGTGAG CAAAATACCT TAGTTTTATT CTTACAGGAT TGATACATTT 
AATGAATAAG TAGGTAAACA TGACATCAAT TATATTCTTG ACAAATTGTA ATAATCTGAA 
AGAGGAGAAG TCAAATAACC TTTAGATGCT AAATTAAAGC TTGAACTAAA ATAGTATTAT 
GAAACTTACT TAAAAAAATA TGAATAAACA ATTGATAAAT ATAAATACAT TAAGAATAAA 
TGGGAAGAAA TTGTAGACTG TGTAAAATTA CTATCTAAAT AAATAAATCT TCCAAATAAA 
AAAGTATGGG AGGAGAAGAA TACAATTCTA CATTTGCTTG CTCAAATTTG TGCGTACTGG 
ACTCTAATTG ATAGCGGAGA ATAAAAGTAA GGCAAAAAAG ATATCTTTAA AAAACCAAAC 
CATACTCAAA TAATTAGCAT TATAATGCTT TTAGGAATTC ATAGAGAAAT TGGATTATGG 
CAAGGAGTTA AAAATTACTT TTCAAGAGGC AGCTAAATTT AATTTAAAAA TTAATTGATT 
GAGATTTTGA CTGGAGAAGG AAAATCAATC ACATTAGGAT TTTGCTCTTT AACACTTGCC 
TTGTTGGGTT GTGAGGTTGA TGTAGTTTGC TATAGTTCCT ACCTTTCAGA GAGAGATGAT 
AAAGACTTTC GTAAGCTCTT TAACTTATTT GGAGTTTCTG ACAAAATCAA ATATGGAACT 
TTTAAATAAT AAGCAGAAAG ATACCTAGAA AGCTATTTAG ATGTTAGGCA AAATACCTTA 
AATATAATAA ATTAAAGCAG GTAAAATGAT AACAGAAATC CTTTACAAAA TACTATCAAT 
AATAAAATAT TGTTAATTGA TGAAGTAGAT ATCTTTTTCA ATCTAGATTA TTATGGAAAA 
ACTTACAATC CTTCAGTAAG TTTTTAGACT TAAGAAATAG TAAATATAAT TAAGGAAATT 
TGGAATTAGA GAGGAAAATA AGAATCAAGC ATTATTACGA ATATTTTATA AAGTAGTAGC 
TATAAACAAT TAATCAACAA ATATCCAGCA TATGAAAAAC TTTTCTAAAG ACATATTAAA 
TAACTTGCTG GAGATGTGAA TAATGTATAA TAGCATAAAA AAAATAAAAA CTATGTAGTT 
AGAAATAATT AAATTGGGTA TAGATAAAAC GATTCAACAA TTTCTTTCAG TACATGTTAT 
AATTACTTAA CTCTTTTTGC ATATTTCCTT GAAAATTCTA ATGGAAATAT AGATAACTAG 
GCACTTTAAA AAGAAATTTC ATTAAATATT GAATGCGGAA ATATTTCTTA TGCACTATTA 
CCAGATACTT ATTCAGCTAT ATTAGGGGTA ACCGGTACTC TTTAATCTTT AAATTAACAA 
ATGAAACAGT GTCTCAAAAA GTATAAATTC TCAACAGAAA TTTATATCCC TTCAATGTTT 
GGAAATTCCA AGCTAGATTT TAGAGAAGGA GATATGGTAA AAGTAGAAAT AGATTTAGAA 
AACTAATATT TGAAAATTCA GGAGATCTCT TTCTCAGCAA TTAAATAATA ATAATCTGTG 
CTCATATTCT TTAAAGATAG TTAATCTTTA TTATAATATC ATGAAAGCAA TTATTTATGC 
TTTTCTAAAG AAAAGTACAT TGTAGTTACT GATCATGAAA GTAGTGAAGA TTTTAATTTT 
TTAATTCAAA AAGTGACTAG GTCAGGTCAA ATTGGACTTT TTGTTAGAGA ATATGGGAGA 
GGAACAGATT TTATTTCATT AGATCCTAAT GTAAATAATT CAGATGGTGT AGTAGTCATC 
TAAACATTTC TTTCAGATAA CAAAACAGAA GAAATCCAAA TCAAAGGAAG AACTGCAAGG 
CAAGGCTAAA AAGGATAATA TTACTTAATC TTAAATCAAT AAGATTTAAT AAAAGATTTT 
GGATTGTAAG ATTATCAAAT TTAGTAATGG AAAAATAATT AAATTAATAA GTCTTTTTAT 
GCTAATCTTA ATGAAGTAAG AAATGCTAAA GAAAATAAAA TGTATGCAAA TATTGAAAAT 
AATATAATGT AAGCTGCTAA TTTGCACTAA AAAAGTATTG AATATATTTA GAATATTAAA 
AATTAAAAAT ACAATTAAGC TATTAGCTAT ATTAATGATA ACTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001014266.1
CDS	1..7248
Translation	MNQPEISNRENNLDAKKLIELFEYEDYSELKDKDIILLAGPTGSGKSTTANYLLGYEMFFEKIEIEKEVDGEIYSETEVFNSNGIFEIGHSGKSHTSTLKAHLQGNYFLCDSPGFFDNRGTEIEIANAYSLKFMLNCNSVKMVVLISFFELEARRGALFEIFKLVTRILNPEEFEKIIFLFTKVPNDKSFKTINSKILDFLDIMKNEEDRESFTMKTFLKVILNLKINSNLVIVNPADGNHRLLLKILSHSFSNPAEEFCFSVSENANIKLYTEETSKIILK*YGKYMEVKNRMVEFKIRDYTGNEFLLYSYQDLSKEIVRIFEKKNAFNTFKEASESNIFSSQNIKEILDIISYLMIDEFKAEVFEGFKTSENQFKEIEQVCFEISKRITEDFPNIENVKNNLKLKDFSDTSLGLIYKTNEIRDKYLLEIEDLKLSERFYIRT*LEVNSIKDSVTNIAKIGFIKLIEKFLKAFILNEGENFYETIVQLHKQKNNGLLSNLKLIIADITEEIKPSMDDSEIVFNPLECLPNFEISILKINNSKYSEYIPDCEILYNEALLHLKEFNQVFNFIKENSEDFHLHKLNILKILDEIRNQDDLIGKTADEYRIKDEIKQKINKKAREAKKILNYLEIEEEENNCDEEEDEDDINSIKSNDLVKNFKLK*NDFKLLKAYNFLSDMHWTDYFTKFTTSINKLEVKLNKYLECKNVFNLIKNDKFCIKKVFLRISCISVFKDVVKNYEGQYQMCVDSVKKKVSELNEQIKKFISCKRGDNIFNSEGWEKLNYILLLSKIETLDKIADVKGDSNSSLIEIKCLITYFEENFQLIKNLSRAKVNPNDEDFTISNFVKQAKLINIEYQYIKSLLKRFSFELMALVNVYDEMVTDYISKKEYIENLEIGSYDRINRFISMSEFDFLKEKFKSLPRPKETI*VFKNLENLIKNEDFNLDSEIYLLSQLKSDTDKALDKSFKIYDKLYNEI*VKSYFYFKNGGFSENIRKNLKIVSEILRVLEKYKNQLY*SRNELNIFLNIFRNYIKSKMIFSKIKQLKFEMEYELMTFTGLSEFKYIFDDWNTFSSEINIKEEISKSILNLHHLSKELIYLLNLLGLNEAKHPSDFSETFQELQNSQEIKLVFNNYETSIRNKIKNKDSFIKTDLKVLKNFAKAENIELNYCDFVKLFIEEEIYEESNKNYEINLLVKKDYDAKKQLLQIKANEQFFDRIIKNMIMISKATDIKDQFPFIEVSCFNTFLTALNNLKIIKHFVNDEYLQHETTITKLITHIFTSRKFFEECDKILIYNDQSFTYNLINLFKHHSFFEVIQRETVGAEAKPLDLFKSILVKVKEGLLKVKAQQLIKEKMVDQALNKNWKKFNALNQILKDSPLFESEELKKIKEIGLQILNDDLVKEIKNVESNDAFTALENCNFRVKEIIFSIRKYSINEFNTEFIDINSMLKKIEKVNQIVQEALNIVKASIIYDKMNDLMNMIYNADEHLKSIDYPYEPIIVLKELTNKLDQEISDLPKSFMYSNLKELSQFLISLQKISDVSKFTIVDKKLFEAFEIIMTLGEDVIFELGNILKQDASGAQIVSKIPFYSYRIDTFNEVGKHDINYILDKL*SERGEVKPLDAKLKLELKYYETYLKKYETIDKYKYIKNKWEEIVDCVKLLSKINLPNKKVWEEKNTILHLLAQICAYWTLIDSGEKGKKDIFKKPNHTQIISIIMLLGIHREIGLWQGVKNYFSRGSIFKNLIEILTGEGKSITLGFCSLTLALLGCEVDVVCYSSYLSERDDKDFRKLFNLFGVSDKIKYGTFK*AERYLESYLDVRQNTLNIINSRNDNRNPLQNTINNKILLIDEVDIFFNLDYYGKTYNPSVSFTEIVNIIKEIWNRGKESSIITNILSSSYKQLINKYPAYEKLFRHIKLAGDVNNV*HKKNKNYVVRNNIGYRNDSTISFSTCYNYLTLFAYFLENSNGNIDNALKEISLNIECGNISYALLPDTYSAILGVTGTLSLNQMKQCLKKYKFSTEIYIPSMFGNSKLDFREGDMVKVEIDLENYLKIQEISFSAIK**SVLIFFKDSSLLYHESNYLCFSKEKYIVVTDHESSEDFNFLIQKVTRSGQIGLFVREYGRGTDFISLDPNVNNSDGVVVITFLSDNKTEEIQIKGRTARQGKGYYLILNQDLIKDFGLDYQI*WKNNINKSFYANLNEVRNAKENKMYANIENNIMAANLHKSIEYINIKNKYN*AISYINDNC

Target ORF information:

RefSeq Version	XM_001014266.1
Organism	Tetrahymena thermophila SB210
Definition	Tetrahymena thermophila SB210 helicase carboxy-terminal domain protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001014266.1

ORF Insert Sequence:

ATGAATCAGC CAGAAATATC AAATAGAGAA AACAATTTAG ATGCCAAAAA ATTGATAGAA 
TTATTCTAGG AATACGAATA AGACTATAGC GAATTAAAAG ATAAGGATAT AATCTTATTA 
GCAGGACCTA CTGGAAGCGG AAAGAGTACT ACTGCTAACT ATCTTTTAGG TTATGAAATG 
TTTTTTGAAA AGATTGAAAT AGAGAAAGAA GTAGATGGTG AAATCTATTA AAGTGAAACT 
GAAGTTTTTA ATTCAAATGG AATATTTGAA ATTGGGCACT CTGGAAAATC ACATACTTCA 
ACACTGTAAA AAGCTCATTT ACAAGGTAAT TATTTTTTGT GCGATTCTCC TGGATTTTTT 
GATAATCGTG GTACTGAAAT CGAAATCGCT AATGCATATT CTTTAAAGTA ATTTATGTTG 
AATTGCAATA GTGTGAAAAT GGTTGTTTTG ATAAGCTTTT TTGAATTAGA GGCTCGAAGA 
GGAGCATTAT TTTAAGAGAT ATTTAAACTT GTAACTAGAA TTCTTTAAAA CCCAGAGTAA 
GAGTAATTTG AAAAGATAAT ATTTCTATTT ACTAAGGTTC CTAATGATAA AAGCTTTAAA 
ACTATTAATA GTAAGATTCT AGATTTTCTT GATATTATGA AAAACTAAGA AGAAGATAGG 
GAATCATTTA CAATGAAAAC CTTTTTGAAA GTTATTTTAA ATTAACTTAA AATCAATAGT 
AATTTAGTAA TTGTAAATCC TGCTGATGGA AATCATAGGT TATTACTTTA AAAGATCCTT 
AGCCATTAAT CTTTCAGTAA TCCTGCTGAA GAATTTTGCT TTAGTGTTTC AGAAAATGCA 
AATTAATAGA TAAAGTTATA CACAGAGGAG ACGAGCAAAA TTATATTAAA ATAATAATAA 
TATGGAAAGT ATATGGAAGT AAAGAATAGA ATGGTTGAAT TCAAGTAAAT AAGGGACTAT 
ACAGGGAATG AATTTTTGTT ATATTCATAT CAAGATTTAT CGAAAGAAAT TGTAAGAATC 
TTTGAAAAAT AAAAAAACTA GGCTTTTAAC ACTTTTAAGG AAGCTTCTGA ATAAAGCAAC 
ATATTTTCCT CCCAAAATAT TAAAGAAATA CTAGATATTA TTTCATATTT ATAAATGATT 
GATGAATTTA AAGCTGAAGT TTTTGAATAA GGCTAATTTA AAACTAGCGA AAATCAATTC 
AAATAAGAGA TTGAACAAGT TTGCTTTGAG ATAAGTAAAA GGATAACTGA AGATTTCCCT 
TAAAACATCG AAAATGTTAA GAATAATCTA TAAAAATTAA AAGATTTTTC AGATTAAACT 
TCTTTAGGTT TGTAAATATA TAAGTAAACC TAAAATGAAA TAAGGGATAA ATATTTGTTA 
GAAATAGAAG ACTTGAAATA ATTGTCTGAA AGATTTTACA TAAGGACATA ATAATTAGAG 
GTTAACTCAA TCAAAGATAG TGTAACCAAT ATAGCTTAGA AAATTGGCTT TATAAAATTA 
ATTGAAAAAT TCCTCAAAGC TTTTATCTTA AATGAAGGCG AAAATTTTTA CGAAACTATA 
GTTCAGTTAC ATAAGCAAAA AAATAATGGG CTGTTATCAA ATCTAAAGTT AATAATTGCC 
GATATTACTG AAGAGATTTA AAAATAACCC TCTATGGATG ACTCTGAGAT TGTTTTTAAT 
CCTTAATTAG AGTGCCTGCC AAATTTTGAA ATTAGCATAC TCAAAATATA AAACTAGAAT 
AGCAAATATT CTGAATATAT TCCAGACTGC GAGATTTTAT ATAATGAAGC TCTTTTACAT 
TTAAAAGAAT TTAATTAACA AGTATTTAAT TTTATCAAAG AAAATTCTTA AGAAGATTTT 
CATTTGCATA AACTATAAAA TATCTTAAAA ATATTAGACG AAATCAGAAA TCAAGATGAT 
TTGATTTAAG GTAAAACTGC TGACGAATAT AGATAAATCA AGGATGAAAT AAAACAAAAA 
ATTAATAAAA AGGCTCGTGA AGCTAAAAAA ATTTTAAATT AATATTTATA GGAAATTGAA 
GAAGAAGAAA ACAACTGTGA CGAAGAAGAA GATGAAGATG ATATTAATTC AATAAAATCA 
AATGATTTAG TAAAAAACTT TAAATTAAAG TAATAAAATG ACTTTAAGTA GTTGTTGAAG 
GCATACAATT TTCTTAGTGA TATGCATTGG ACAGATTAAT ATTTTTAAAC AAAATTTACA 
TAAACTAGCA TAAATAAACT AGAAGTAAAG TTAAATAAAT ATTTAGAGTA GTGCAAGTAG 
AATGTTTTTA ACTTGATCAA AAATGATAAA TTCTGTTAAA TAAAAAAAGT CTTTTTAAGA 
ATAAGTTGCA TTAGTGTTTT TAAAGATGTA GTTAAAAACT ATGAAGGACA ATATCAGATG 
TGTGTAGATT CAGTGAAGAA AAAAGTATCA GAGTTGAATG AACAAATAAA AAAATTTATT 
AGTTGTAAGA GAGGAGATAA TATATTTAAT AGTGAAGGCT GGGAAAAATT AAATTAATAT 
ATATTGCTTT TGTCTAAGAT TGAGACTTTA GACAAGATTG CTGATGTCAA GGGAGATTCT 
AATTCTTCAC TAATTGAAAT CAAGTGTTTA ATCTAGACAT ATTTTGAAGA AAATTTCCAA 
TTAATAAAGA ATTTATCAAG AGCTAAAGTT AACCCAAATG ACGAAGATTT CACAATTTAA 
TCTAATTTTG TTAAACAAGC GAAATTAATA AATATAGAAT ATCAATATAT TAAGTCGCTT 
TTAAAAAGAT TCTCTTTTGA ATAACTTATG GCTTTGGTTT AAAACGTTTA CGATGAGATG 
GTCACTGATT ACATTTCTAA GAAAGAATAT ATTGAGAATC TTTAAGAGAT TTAAGGGAGC 
TATGATAGAA TTAACAGATT TATCTAAAGC ATGAGTGAAT TTGATTTCTT AAAAGAGAAA 
TTTAAAAGTT TGCCTAGACC AAAAGAGACT ATATAGTAGG TATTTAAGAA CCTTGAAAAT 
TTGATAAAAA ATGAAGATTT TTAGAACCTA GATTCAGAAA TATACTTATT GAGCCAACTT 
AAAAGCGATA CTGATAAATA AGCTTTAGAT AAATCATTTT AAAAAATATA CGACAAGCTC 
TATAATGAAA TATAATAAGT AAAGAGTTAT TTTTATTTTA AATAAAATGG AGGTTTCTCT 
GAGAATATTA GAAAAAATCT GAAGATTGTA TCTGAAATAT TAAGAGTGCT CGAGAAATAT 
AAGAATCAGC TTTACTAATA ATCTCGTAAT GAATTAAATA TTTTTTTGAA TATCTTTAGG 
TAAAATTACA TAAAGTCTTA AAAGTAAATG ATTTTTAGTA AAATAAAACA ACTAAAATTC 
TAAGAAATGG AATAGTAATA CGAATAATTA ATGTAAACTT TTACTGGCTT GAGTGAATTT 
AAATACATTT TCTAATAAGA TGATTGGAAT ACTTTTAGCT CAGAAATTTA AAACATAAAA 
GAGGAAATTT CAAAGTCTAT TTTAAATCTT CATCATCTTT CGAAAGAGTT AATATATCTT 
TTAAATTTGC TTTAAGGACT AAATGAAGCT TAGAAGCACC CTTCAGATTT TTCAGAGACA 
TTTTAACAAG AATTACAAAA TTCCCAATAA GAAATTAAGT TAGTATTTAA TAATTATGAA 
ACGAGCATTA GAAATAAAAT AAAAAATAAG GATAGCTTTA TTAAAACTGA CTTAAAAGTT 
TTGAAAAATT TTGCTTAGAA AGCAGAAAAT ATTGAATAAT TGAATTATTG TGATTTTTAA 
GTTAAACTAT TTTAAATTGA AGAAGAAATA TATGAAGAAT CAAATAAGAA TTATTAAGAA 
ATTTAAAATT TATTGGTTAA AAAGGATTAT GACTAGGCTA AAAAACAGCT TCTACAAATT 
AAGTAGGCAA ATGAGCAGTT TTTCGATAGA ATAATAAAAA ATATGTAAAT TATGATATCC 
AAAGCTACAG ATTAAATTAA AGACCAATTC CCCTTTATAG AAGTTTCATG CTTCTAGAAT 
ACTTTTTTAA CTGCCTTAAA CAACCTGAAA ATAATAAAAC ACTTTGTAAA TTAAGATGAG 
TATCTTCAAC ATGAAACAAC AATTACTAAA CTTATTACTC ATATATTTAC ATAATCAAGG 
AAGTTCTTTG AGGAATGCGA TAAAATTCTG ATCTACAATG ATCAATCGTT TACATATTAA 
AATTTAATAA ATCTATTTAA ACATCACTCA TTTTTTGAAG TAATTCAAAG AGAGACGGTT 
TAAGGAGCAG AGTAAGCAAA GCCTTTATAA GATCTTTTTA AAAGTATTTT AGTTAAAGTA 
AAAGAAGGAC TATTAAAAGT AAAATAGGCT CAACAATTAA TTAAAGAGAA AATGTAAGTT 
GATCAAGCCT TAAATAAAAA TTGGAAAAAA TTCAATGCTC TTAATCAAAT CCTAAAGGAC 
TCACCTTAAT TGTTTGAATA ATCTGAAGAA CTTAAAAAAA TAAAAGAAAT TGGTCTTCAA 
ATTTTGAATG ACGATTAACT AGTAAAGGAA ATTTAAAAAA ATGTTGAAAG CAACGATTAA 
GCTTTTTAAA CTGCATTGGA AAATTGCAAT TTCTAAAGAG TTAAAGAGAT CATTTTTAGC 
ATAAGGAAAT ACTCTTAAAT AAATTAAGAA TTTAATACTG AATTCATCGA CATTAATTCC 
ATGCTAAAAA AAATTGAGTA AAAGGTTAAT CAAATAGTGC AAGAAGCACT AAATATAGTG 
AAAGCTAGTA TTATTTATGA CTAAAAAATG AATGATTTAA TGAATATGAT TTATAATGCA 
GATGAGCATT TGAAAAGCAT AGATTAATAT CCTTATGAAC CTTAAATCTA AATAGTTTTA 
AAAGAGCTTA CTAATAAATT AGATCAAGAA ATCTCTGATC TTCCTAAATA GAGCTTTATG 
TATTAATCAA ACCTAAAAGA ATTAAGCCAA TTTTTAATTA GCTTACAAAA AATTTCTGAT 
TAAGTGAGTA AATTCACTTA AATTGTTGAT AAAAAGTTAT TTGAAGCTTT TGAAATCATA 
ATGTAAACTT TGGGAGAAGA TGTAATATTT GAATTAGGTA ATATTCTAAA GCAAGATGCT 
TCTGGGGCTC AGATTGTGAG CAAAATACCT TAGTTTTATT CTTACAGGAT TGATACATTT 
AATGAATAAG TAGGTAAACA TGACATCAAT TATATTCTTG ACAAATTGTA ATAATCTGAA 
AGAGGAGAAG TCAAATAACC TTTAGATGCT AAATTAAAGC TTGAACTAAA ATAGTATTAT 
GAAACTTACT TAAAAAAATA TGAATAAACA ATTGATAAAT ATAAATACAT TAAGAATAAA 
TGGGAAGAAA TTGTAGACTG TGTAAAATTA CTATCTAAAT AAATAAATCT TCCAAATAAA 
AAAGTATGGG AGGAGAAGAA TACAATTCTA CATTTGCTTG CTCAAATTTG TGCGTACTGG 
ACTCTAATTG ATAGCGGAGA ATAAAAGTAA GGCAAAAAAG ATATCTTTAA AAAACCAAAC 
CATACTCAAA TAATTAGCAT TATAATGCTT TTAGGAATTC ATAGAGAAAT TGGATTATGG 
CAAGGAGTTA AAAATTACTT TTCAAGAGGC AGCTAAATTT AATTTAAAAA TTAATTGATT 
GAGATTTTGA CTGGAGAAGG AAAATCAATC ACATTAGGAT TTTGCTCTTT AACACTTGCC 
TTGTTGGGTT GTGAGGTTGA TGTAGTTTGC TATAGTTCCT ACCTTTCAGA GAGAGATGAT 
AAAGACTTTC GTAAGCTCTT TAACTTATTT GGAGTTTCTG ACAAAATCAA ATATGGAACT 
TTTAAATAAT AAGCAGAAAG ATACCTAGAA AGCTATTTAG ATGTTAGGCA AAATACCTTA 
AATATAATAA ATTAAAGCAG GTAAAATGAT AACAGAAATC CTTTACAAAA TACTATCAAT 
AATAAAATAT TGTTAATTGA TGAAGTAGAT ATCTTTTTCA ATCTAGATTA TTATGGAAAA 
ACTTACAATC CTTCAGTAAG TTTTTAGACT TAAGAAATAG TAAATATAAT TAAGGAAATT 
TGGAATTAGA GAGGAAAATA AGAATCAAGC ATTATTACGA ATATTTTATA AAGTAGTAGC 
TATAAACAAT TAATCAACAA ATATCCAGCA TATGAAAAAC TTTTCTAAAG ACATATTAAA 
TAACTTGCTG GAGATGTGAA TAATGTATAA TAGCATAAAA AAAATAAAAA CTATGTAGTT 
AGAAATAATT AAATTGGGTA TAGATAAAAC GATTCAACAA TTTCTTTCAG TACATGTTAT 
AATTACTTAA CTCTTTTTGC ATATTTCCTT GAAAATTCTA ATGGAAATAT AGATAACTAG 
GCACTTTAAA AAGAAATTTC ATTAAATATT GAATGCGGAA ATATTTCTTA TGCACTATTA 
CCAGATACTT ATTCAGCTAT ATTAGGGGTA ACCGGTACTC TTTAATCTTT AAATTAACAA 
ATGAAACAGT GTCTCAAAAA GTATAAATTC TCAACAGAAA TTTATATCCC TTCAATGTTT 
GGAAATTCCA AGCTAGATTT TAGAGAAGGA GATATGGTAA AAGTAGAAAT AGATTTAGAA 
AACTAATATT TGAAAATTCA GGAGATCTCT TTCTCAGCAA TTAAATAATA ATAATCTGTG 
CTCATATTCT TTAAAGATAG TTAATCTTTA TTATAATATC ATGAAAGCAA TTATTTATGC 
TTTTCTAAAG AAAAGTACAT TGTAGTTACT GATCATGAAA GTAGTGAAGA TTTTAATTTT 
TTAATTCAAA AAGTGACTAG GTCAGGTCAA ATTGGACTTT TTGTTAGAGA ATATGGGAGA 
GGAACAGATT TTATTTCATT AGATCCTAAT GTAAATAATT CAGATGGTGT AGTAGTCATC 
TAAACATTTC TTTCAGATAA CAAAACAGAA GAAATCCAAA TCAAAGGAAG AACTGCAAGG 
CAAGGCTAAA AAGGATAATA TTACTTAATC TTAAATCAAT AAGATTTAAT AAAAGATTTT 
GGATTGTAAG ATTATCAAAT TTAGTAATGG AAAAATAATT AAATTAATAA GTCTTTTTAT 
GCTAATCTTA ATGAAGTAAG AAATGCTAAA GAAAATAAAA TGTATGCAAA TATTGAAAAT 
AATATAATGT AAGCTGCTAA TTTGCACTAA AAAAGTATTG AATATATTTA GAATATTAAA 
AATTAAAAAT ACAATTAAGC TATTAGCTAT ATTAATGATA ACTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TTHERM_00227350 cDNA ORF clone, Tetrahymena thermophila SB210

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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