LOC101077362 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101077362
Entrez Gene ID	101077362
Full Name	clathrin heavy chain 1-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029829715.1	XP_029685575.1	clathrin heavy chain 1-like isoform X2
XM_003975152.2	XP_003975201.1	clathrin heavy chain 1-like
XM_029829714.1	XP_029685574.1	clathrin heavy chain 1-like isoform X1
XM_003975152.3	XP_003975201.1	clathrin heavy chain 1-like isoform X4
XM_029829716.1	XP_029685576.1	clathrin heavy chain 1-like isoform X3
XM_029829717.1	XP_029685577.1	clathrin heavy chain 1-like isoform X5

Pathways from BioSystems
Gene Ontology

The following LOC101077362 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101077362 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo14008	XM_003975152.2 Latest version!	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo60673	XM_029829714.1 Latest version!	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo60674	XM_029829715.1 Latest version!	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo60675	XM_029829716.1 Latest version!	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo60676	XM_003975152.3 Latest version!	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo60677	XM_029829717.1 Latest version!	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101077362 ( XM_003975152.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003975201.1 Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), mRNA.

Price & Availability↑

CloneID	OTo14008
Clone ID Related Accession (Same CDS sequence)	XM_003975152.2
Accession Version	XM_003975152.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	clathrin heavy chain 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071157.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003975152.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG CTGCCAAGAC CCTGCAGATC TTTAACATTG AGATGAAAAG TAAACTAAAG 
GCTCACACCA TGACAGAAGA TGTGATGTTC TGGAAGTGGA TATCGGTGAA CACCGTCGCC 
CTGGTGACTG ATTCAGCCGT CTATCACTGG AGCATGGAGG GAGACTCTCA GCCCGCTAAG 
GTTTTCGACC GGCACGCCAG TCTGGCAGGC TGTCAGATCA TCAACTACAG AACTGATCAG 
CAACAGAAGT GGCTGCTTTT GATCGGGATC TCCGCGCAGC AAAACCGCGT GGTTGGAGCC 
ATGCAGCTGT ATTCAGTCGA CAGGAAAGTG TCCCAGCCCA TCGAGGGACA CGCTGCTGCC 
TTTGGAGACT TCAAAGTGGA GGGGAACACC AAACCTTCCA CCCTCTTTTG CTTCGCCGTG 
CGCTCGCAAG CTGGGGGAAA GTTGCACATC ATTGAAGTCG GTCAGCCAGC TGCGGGGAAC 
CAGCCCTTTG CTAAGAAAGC CGTTGATGTG TTTTTCCCTC CAGAGGCGCA GACAGACTTT 
CCCGTAGCCA TGCAGATCGG AAGTAAACAT GGAGTCATAT ATTTGATCAC AAAGTATGGT 
TACATTCACT TGTACGACCT GGAGTCTGGA GTTTGCATCT ACATGAATCG AATTAGCGCG 
GAGACCATTT TTGTCACGGC TCCACATGAA GCCACCTCAG GAATCATTGG GGTGAACAAG 
AAGGGTCAGG TCCTCTCAGT CTGCGTTGAA GAGGAAAACA TCGTCAACTA CGCCACCAAC 
GTTTTACAGA ACCCAGACCT GGCGTTGAGG ATAGCTTTAA GGTCCAATCT CGCTGGGGCG 
GAGGAGATTT TTGCGAGGAA GTTCAACACA CTGTTCTCCC AGGGAAATTA TTCAGAGGCT 
GCAAAGGTTG CTGCCTCGGC ACCTAAGGGA ATTCTCCGCA CTGCAGAGAC CATTCGTAAG 
TTCCAGAGCG TCCCAGCTCA GCCAGGTCAG GCCTCCCCAT TGTTGCAGTA CTTTGGTATT 
CTGCTGGACC AGGGTCAGCT CAACAAGTTT GAGTCTTTGG AGCTGTGCCG GCCTGTCCTG 
CAGCAGGGCC GCAAGCAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGTT GGAGTGCTCA 
GAAGAGCTGG GAGACCTGGT GAAGGCATCG GATCCCACTT TAGCTCTTAG CGTGTACCTC 
AGAGCTAACG TCCCCAACAA GGTCATCCAG TGTTTTGCTG AAACTGGCCA GTTCCAGAAG 
ATTGTGCTTT ATGCCAAAAA GGTGGGCTAC ACCCCTGACT GGGTTTTCCT CCTGAGAAAC 
GTGATGCGTG TAAATCCAGA CCAGGGCCTG CAGTTTGCCC AGATGCTTGT CCAGGACGAG 
GAGCCGCTGG CCAACATAAA CCAGATTGTT GACGTCTTCA TGGAGGGAAA TCTGATCCAG 
CAGTGCACGT CCTTCCTGTT GGACGCTCTG AAGAACAACC GTCCCGCCGA AGGACACTTA 
CAGACTCGCC TGCTTGAGAT GAACCTCATA CATGCCCCCC AGGTCGCGGA TGCCATCCTT 
GGGAACCAGA TGTTCACCCA TTATGACCGG GCCCATGTGG CCCAGCTGTG CGAGAAGGCA 
GGTCTCCTGC AAAGAGCTCT TGAACACTAC ACAGATCTGT ACGACATCAA ACGGGCAGTG 
GTGCACACGC ATCTGCTCAA CCCTGAGTGG CTGGTGAACT TTTTTGGCTC GTTGTCAGTT 
GAAGACTCTT TGGAATGTCT GAGAGCCATG TTGTCAGCTA ACATCAGGCA GAACCTGCAG 
CTCTGCGTCC AGGTAGCATC GAAGTATCAC GAGCAGCTGG GGACTCAGTC TTTAGTGGAA 
CTCTTTGAAT CCTTCAAGAG TTATGAGGGT TTGTTTTACT TCCTTGGATC GATTGTGAAT 
TTCAGTCAGG AGCCTGATGT TCACTTTAAG TACATCCAGG CTGCCTGCAA AACAGGTCAG 
ATCAAGGAAG TGGAGAGAAT CTGTCGAGAG AGCAACTGCT ACGACCCAGA GAGAGTCAAA 
AACTTCCTCA AGGAGGCCAA GCTTACAGAT CAGCTCCCTC TGATCATCGT GTGTGATCGC 
TTTGACTTTG TTCACGACTT GGTGCTCTAT CTCTACCGCA ACAATCTCCA GAAATACATT 
GAAATCTACG TGCAAAAGGT GAATCCCAGT CGTTTACCGG TGGTTATAGG TGGACTGTTG 
GATGTCGACT GTGCTGAGGA TGTGATCAAG AACCTGATCA TTGTGGTCAG AGGGCAGTTT 
TCCACTGATG AGCTAGTAGA GGAAGTAGAA AAGAGGAACA GGTTAAAGCT GTTGTTACCA 
TGGCTTGAGT CTCGCATCCA TGAAGGGTGC GAGGAACCGG CCACTCACAA TGCGCTGGCC 
AAGATCTATA TTGATAGCAA CAATACGCCC GAGCGTTTCC TGAAAGAGAA CCCATTCTAC 
GACAGCGCTG TTGTTGGAAA ATACTGTGAG AAGAGAGACC CTCACCTGGC CTGTGTAGCT 
TATGAGAGAG GACAATGTGA CCTGGACCTT ATAAAGGTGT GCAATGAAAA CTCCTTATTC 
AAGAGTGAGG CTAGATATCT GGTCCATCGG AAAGACCCAG AGCTTTGGGC AAATGTGCTT 
GAAGAAAACA ACCCATTCAG ACGACAACTT ATCGATCAGG TGGTGCAGAC AGCCCTGTCA 
GAGACCCAGG ACCCAGAGGA GGTTTCAGTA ACCGTCAAGG CCTTCATGAC TGCAGACCTT 
CCCAATGAGC TGATTGAACT GCTGGAAAAG ATCGTACTCG ACAACTCTGT CTTTAGCGAG 
CACAGAAACC TCCAGAACCT GCTCATTCTG ACTGCCATCA AGGCCGACCG TACTCGGGTG 
ATGGAGTACA TTAACAGACT AGACAACTAC GACGCTCCGG ACATTGCTAA TATCGCCATC 
AGCAATGAGC TGTTTGAAGA AGCGTTTGCC ATTTTCAAGA AGTTTGATGT CAACACCTCT 
GCAATACAGG TATTGATAGA GCACATAGGG AACCTGGACC GAGCCTACGA GTTTGCAGAG 
CGCTGTAACG AACCTGCGGT GTGGAGCCAG TTGGGCAGAG CTCAGCTGCA CCGAGGCCTC 
GTCAAGGAGG CCATCGACTC GTACATCAAA GCTGTCGACC CTTCTGCGTA CATGGAGGTG 
GTCAATGCAG CCAGCAAGAA CAATAATTGG GAAGATCTGG TGAAATTCCT CCAGATGGCT 
CGAAAGAAAG CCAGAGAGTC CTACGTGGAG ACGGAGCTCA TCTTCGCCTT GGCTAAAACA 
AACCGTCTGG CCGAACTGGA GGAGTTCGTC AGTGGACCCA ACAATGCTCA CATCCAGCAG 
GTGGGCGATA GATGCTACGA GGAAGGCATG TACGAGGCAG CGAAGCTCTT GTATAACAAC 
GTTTCCAACT TTGCTCGTCT CGCCTCGACA CTCGTACATC TCGGGGAGTA CCAGGCGGCC 
GTGGACAGTG CCAGGAAAGC CAACAGCACA CGGACATGGA AGGAGGTCTG CTTTGCGTGC 
GTGGATGGGG AGGAGTTCCG TCTGGCGCAA ATCTGCGGCC TCCACATCGT CATCCACGCT 
GACGAGCTGG AAGACCTGAT CAGCTACTAT CAGGACCGCG GATACTTCGA GGAACTCATT 
GCGCTGCTGG AGGCGGCGCT GGGTTTGGAG CGGGCTCACA TGGGCATGTT CACAGAGCTC 
GCCATCCTCT ACTCCAAGTT CAAACCTCAG AAGATGAGGG AACACCTGGA GCTCTTCTGG 
TCCAGAGTCA ATATTCCAAA GGTCCTCCGT GCAGCAGAGC AGTCTCACCT CTGGGCAGAG 
CTGGTTTTCC TTTACGATAA ATACGAGGAG TACGACAACG CAGTCCTCAC GATGATGTCT 
CATCCGACTG AAGCTTGGAA AGAAGGGCTG TTCAAAGACA TTATCCCAAA AGTGGCCAAT 
GTGGAACTGT ACTATAAATC CCTCTCCTTC TATCTGGACT ATAAACCGCT CCTACTCAAC 
GACCTGCTGA CCATCTTGTC TCCACGGCTT GATCACAGCC GAGCGGTCTC GTTTTTCTCA 
AAGATGAACC AGCTGAAGCT GGTTAAGCCT TACCTGAGAT CAGTCCAGAA CCACAACAAC 
AAGTCTGTCA ACGAGGCACT CAACAACCTG TTGACAGAGG AGGAAGACTA TCAGGGTCTG 
AGAGCGTCCA TCGATGCCTA CGACAACTTT GATACCATTG GCCTGGCGCA GAGATTAGAG 
AAACACGACC TGATCGAGTT CAGACGCATT GCTGCTTATC TTTATAAGGG TAACAACCGC 
TGGAGACAGA GTGTTGAGCT CTGCAAGAAG GACAAACTCT ACAAGGATGC CATGCTGTAC 
GCTGCAGAGT CGAAGGATGC TGAGCTGGCA GAAAATCTGC TTCAGTGGTT CCTGGAGGAA 
GGCAGGAAAG AGTGCTTTGC TGCTTGCCTC TTTGCATCCT ATGACCTGCT GCATCCCGAC 
GTAGTGCTGG AGCTGGCCTG GAGGCACAAT ATCATGGACT TCGCCATGCC GTACTTCATC 
CAGGTCATGA GGGAGTACCT CACAAAGGTG GACAAACTGG AGGAGGCAGA GAGCCAGAGA 
AAAACGGAGG AGGAGGTGAC AGAACCTCAG CCGATGGTGT TTGGCCAGCA GCTGATGTTG 
ACCGCCTCAC CTGCTCCAGT GACCCCCCAG ACGGGCTACC CGAGCTATGC CTACCCCCCC 
GCAGGTTACC CTGCCGCCCC CGCTGCTGGC TACCCCCCTC AACCCCCAGC ATACGGCTTC 
AACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003975201.1
CDS	338..5386
Translation	MAQILPIRFQEHLQLQNMGVNPANIGFSYLTMESDKFICIREKVGDQNQVVIVDMSDPTNPIRRPISADSAIMNPASKVIALKAAKTLQIFNIEMKSKLKAHTMTEDVMFWKWISVNTVALVTDSAVYHWSMEGDSQPAKVFDRHASLAGCQIINYRTDQQQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFGDFKVEGNTKPSTLFCFAVRSQAGGKLHIIEVGQPAAGNQPFAKKAVDVFFPPEAQTDFPVAMQIGSKHGVIYLITKYGYIHLYDLESGVCIYMNRISAETIFVTAPHEATSGIIGVNKKGQVLSVCVEEENIVNYATNVLQNPDLALRIALRSNLAGAEEIFARKFNTLFSQGNYSEAAKVAASAPKGILRTAETIRKFQSVPAQPGQASPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKASDPTLALSVYLRANVPNKVIQCFAETGQFQKIVLYAKKVGYTPDWVFLLRNVMRVNPDQGLQFAQMLVQDEEPLANINQIVDVFMEGNLIQQCTSFLLDALKNNRPAEGHLQTRLLEMNLIHAPQVADAILGNQMFTHYDRAHVAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVEDSLECLRAMLSANIRQNLQLCVQVASKYHEQLGTQSLVELFESFKSYEGLFYFLGSIVNFSQEPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCAEDVIKNLIIVVRGQFSTDELVEEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNTPERFLKENPFYDSAVVGKYCEKRDPHLACVAYERGQCDLDLIKVCNENSLFKSEARYLVHRKDPELWANVLEENNPFRRQLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFKKFDVNTSAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLGRAQLHRGLVKEAIDSYIKAVDPSAYMEVVNAASKNNNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFVSGPNNAHIQQVGDRCYEEGMYEAAKLLYNNVSNFARLASTLVHLGEYQAAVDSARKANSTRTWKEVCFACVDGEEFRLAQICGLHIVIHADELEDLISYYQDRGYFEELIALLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQSHLWAELVFLYDKYEEYDNAVLTMMSHPTEAWKEGLFKDIIPKVANVELYYKSLSFYLDYKPLLLNDLLTILSPRLDHSRAVSFFSKMNQLKLVKPYLRSVQNHNNKSVNEALNNLLTEEEDYQGLRASIDAYDNFDTIGLAQRLEKHDLIEFRRIAAYLYKGNNRWRQSVELCKKDKLYKDAMLYAAESKDAELAENLLQWFLEEGRKECFAACLFASYDLLHPDVVLELAWRHNIMDFAMPYFIQVMREYLTKVDKLEEAESQRKTEEEVTEPQPMVFGQQLMLTASPAPVTPQTGYPSYAYPPAGYPAAPAAGYPPQPPAYGFNM

Target ORF information:

RefSeq Version	XM_003975152.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003975152.2

ORF Insert Sequence:

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG CTGCCAAGAC CCTGCAGATC TTTAACATTG AGATGAAAAG TAAACTAAAG 
GCTCACACCA TGACAGAAGA TGTGATGTTC TGGAAGTGGA TATCGGTGAA CACCGTCGCC 
CTGGTGACTG ATTCAGCCGT CTATCACTGG AGCATGGAGG GAGACTCTCA GCCCGCTAAG 
GTTTTCGACC GGCACGCCAG TCTGGCAGGC TGTCAGATCA TCAACTACAG AACTGATCAG 
CAACAGAAGT GGCTGCTTTT GATCGGGATC TCCGCGCAGC AAAACCGCGT GGTTGGAGCC 
ATGCAGCTGT ATTCAGTCGA CAGGAAAGTG TCCCAGCCCA TCGAGGGACA CGCTGCTGCC 
TTTGGAGACT TCAAAGTGGA GGGGAACACC AAACCTTCCA CCCTCTTTTG CTTCGCCGTG 
CGCTCGCAAG CTGGGGGAAA GTTGCACATC ATTGAAGTCG GTCAGCCAGC TGCGGGGAAC 
CAGCCCTTTG CTAAGAAAGC CGTTGATGTG TTTTTCCCTC CAGAGGCGCA GACAGACTTT 
CCCGTAGCCA TGCAGATCGG AAGTAAACAT GGAGTCATAT ATTTGATCAC AAAGTATGGT 
TACATTCACT TGTACGACCT GGAGTCTGGA GTTTGCATCT ACATGAATCG AATTAGCGCG 
GAGACCATTT TTGTCACGGC TCCACATGAA GCCACCTCAG GAATCATTGG GGTGAACAAG 
AAGGGTCAGG TCCTCTCAGT CTGCGTTGAA GAGGAAAACA TCGTCAACTA CGCCACCAAC 
GTTTTACAGA ACCCAGACCT GGCGTTGAGG ATAGCTTTAA GGTCCAATCT CGCTGGGGCG 
GAGGAGATTT TTGCGAGGAA GTTCAACACA CTGTTCTCCC AGGGAAATTA TTCAGAGGCT 
GCAAAGGTTG CTGCCTCGGC ACCTAAGGGA ATTCTCCGCA CTGCAGAGAC CATTCGTAAG 
TTCCAGAGCG TCCCAGCTCA GCCAGGTCAG GCCTCCCCAT TGTTGCAGTA CTTTGGTATT 
CTGCTGGACC AGGGTCAGCT CAACAAGTTT GAGTCTTTGG AGCTGTGCCG GCCTGTCCTG 
CAGCAGGGCC GCAAGCAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGTT GGAGTGCTCA 
GAAGAGCTGG GAGACCTGGT GAAGGCATCG GATCCCACTT TAGCTCTTAG CGTGTACCTC 
AGAGCTAACG TCCCCAACAA GGTCATCCAG TGTTTTGCTG AAACTGGCCA GTTCCAGAAG 
ATTGTGCTTT ATGCCAAAAA GGTGGGCTAC ACCCCTGACT GGGTTTTCCT CCTGAGAAAC 
GTGATGCGTG TAAATCCAGA CCAGGGCCTG CAGTTTGCCC AGATGCTTGT CCAGGACGAG 
GAGCCGCTGG CCAACATAAA CCAGATTGTT GACGTCTTCA TGGAGGGAAA TCTGATCCAG 
CAGTGCACGT CCTTCCTGTT GGACGCTCTG AAGAACAACC GTCCCGCCGA AGGACACTTA 
CAGACTCGCC TGCTTGAGAT GAACCTCATA CATGCCCCCC AGGTCGCGGA TGCCATCCTT 
GGGAACCAGA TGTTCACCCA TTATGACCGG GCCCATGTGG CCCAGCTGTG CGAGAAGGCA 
GGTCTCCTGC AAAGAGCTCT TGAACACTAC ACAGATCTGT ACGACATCAA ACGGGCAGTG 
GTGCACACGC ATCTGCTCAA CCCTGAGTGG CTGGTGAACT TTTTTGGCTC GTTGTCAGTT 
GAAGACTCTT TGGAATGTCT GAGAGCCATG TTGTCAGCTA ACATCAGGCA GAACCTGCAG 
CTCTGCGTCC AGGTAGCATC GAAGTATCAC GAGCAGCTGG GGACTCAGTC TTTAGTGGAA 
CTCTTTGAAT CCTTCAAGAG TTATGAGGGT TTGTTTTACT TCCTTGGATC GATTGTGAAT 
TTCAGTCAGG AGCCTGATGT TCACTTTAAG TACATCCAGG CTGCCTGCAA AACAGGTCAG 
ATCAAGGAAG TGGAGAGAAT CTGTCGAGAG AGCAACTGCT ACGACCCAGA GAGAGTCAAA 
AACTTCCTCA AGGAGGCCAA GCTTACAGAT CAGCTCCCTC TGATCATCGT GTGTGATCGC 
TTTGACTTTG TTCACGACTT GGTGCTCTAT CTCTACCGCA ACAATCTCCA GAAATACATT 
GAAATCTACG TGCAAAAGGT GAATCCCAGT CGTTTACCGG TGGTTATAGG TGGACTGTTG 
GATGTCGACT GTGCTGAGGA TGTGATCAAG AACCTGATCA TTGTGGTCAG AGGGCAGTTT 
TCCACTGATG AGCTAGTAGA GGAAGTAGAA AAGAGGAACA GGTTAAAGCT GTTGTTACCA 
TGGCTTGAGT CTCGCATCCA TGAAGGGTGC GAGGAACCGG CCACTCACAA TGCGCTGGCC 
AAGATCTATA TTGATAGCAA CAATACGCCC GAGCGTTTCC TGAAAGAGAA CCCATTCTAC 
GACAGCGCTG TTGTTGGAAA ATACTGTGAG AAGAGAGACC CTCACCTGGC CTGTGTAGCT 
TATGAGAGAG GACAATGTGA CCTGGACCTT ATAAAGGTGT GCAATGAAAA CTCCTTATTC 
AAGAGTGAGG CTAGATATCT GGTCCATCGG AAAGACCCAG AGCTTTGGGC AAATGTGCTT 
GAAGAAAACA ACCCATTCAG ACGACAACTT ATCGATCAGG TGGTGCAGAC AGCCCTGTCA 
GAGACCCAGG ACCCAGAGGA GGTTTCAGTA ACCGTCAAGG CCTTCATGAC TGCAGACCTT 
CCCAATGAGC TGATTGAACT GCTGGAAAAG ATCGTACTCG ACAACTCTGT CTTTAGCGAG 
CACAGAAACC TCCAGAACCT GCTCATTCTG ACTGCCATCA AGGCCGACCG TACTCGGGTG 
ATGGAGTACA TTAACAGACT AGACAACTAC GACGCTCCGG ACATTGCTAA TATCGCCATC 
AGCAATGAGC TGTTTGAAGA AGCGTTTGCC ATTTTCAAGA AGTTTGATGT CAACACCTCT 
GCAATACAGG TATTGATAGA GCACATAGGG AACCTGGACC GAGCCTACGA GTTTGCAGAG 
CGCTGTAACG AACCTGCGGT GTGGAGCCAG TTGGGCAGAG CTCAGCTGCA CCGAGGCCTC 
GTCAAGGAGG CCATCGACTC GTACATCAAA GCTGTCGACC CTTCTGCGTA CATGGAGGTG 
GTCAATGCAG CCAGCAAGAA CAATAATTGG GAAGATCTGG TGAAATTCCT CCAGATGGCT 
CGAAAGAAAG CCAGAGAGTC CTACGTGGAG ACGGAGCTCA TCTTCGCCTT GGCTAAAACA 
AACCGTCTGG CCGAACTGGA GGAGTTCGTC AGTGGACCCA ACAATGCTCA CATCCAGCAG 
GTGGGCGATA GATGCTACGA GGAAGGCATG TACGAGGCAG CGAAGCTCTT GTATAACAAC 
GTTTCCAACT TTGCTCGTCT CGCCTCGACA CTCGTACATC TCGGGGAGTA CCAGGCGGCC 
GTGGACAGTG CCAGGAAAGC CAACAGCACA CGGACATGGA AGGAGGTCTG CTTTGCGTGC 
GTGGATGGGG AGGAGTTCCG TCTGGCGCAA ATCTGCGGCC TCCACATCGT CATCCACGCT 
GACGAGCTGG AAGACCTGAT CAGCTACTAT CAGGACCGCG GATACTTCGA GGAACTCATT 
GCGCTGCTGG AGGCGGCGCT GGGTTTGGAG CGGGCTCACA TGGGCATGTT CACAGAGCTC 
GCCATCCTCT ACTCCAAGTT CAAACCTCAG AAGATGAGGG AACACCTGGA GCTCTTCTGG 
TCCAGAGTCA ATATTCCAAA GGTCCTCCGT GCAGCAGAGC AGTCTCACCT CTGGGCAGAG 
CTGGTTTTCC TTTACGATAA ATACGAGGAG TACGACAACG CAGTCCTCAC GATGATGTCT 
CATCCGACTG AAGCTTGGAA AGAAGGGCTG TTCAAAGACA TTATCCCAAA AGTGGCCAAT 
GTGGAACTGT ACTATAAATC CCTCTCCTTC TATCTGGACT ATAAACCGCT CCTACTCAAC 
GACCTGCTGA CCATCTTGTC TCCACGGCTT GATCACAGCC GAGCGGTCTC GTTTTTCTCA 
AAGATGAACC AGCTGAAGCT GGTTAAGCCT TACCTGAGAT CAGTCCAGAA CCACAACAAC 
AAGTCTGTCA ACGAGGCACT CAACAACCTG TTGACAGAGG AGGAAGACTA TCAGGGTCTG 
AGAGCGTCCA TCGATGCCTA CGACAACTTT GATACCATTG GCCTGGCGCA GAGATTAGAG 
AAACACGACC TGATCGAGTT CAGACGCATT GCTGCTTATC TTTATAAGGG TAACAACCGC 
TGGAGACAGA GTGTTGAGCT CTGCAAGAAG GACAAACTCT ACAAGGATGC CATGCTGTAC 
GCTGCAGAGT CGAAGGATGC TGAGCTGGCA GAAAATCTGC TTCAGTGGTT CCTGGAGGAA 
GGCAGGAAAG AGTGCTTTGC TGCTTGCCTC TTTGCATCCT ATGACCTGCT GCATCCCGAC 
GTAGTGCTGG AGCTGGCCTG GAGGCACAAT ATCATGGACT TCGCCATGCC GTACTTCATC 
CAGGTCATGA GGGAGTACCT CACAAAGGTG GACAAACTGG AGGAGGCAGA GAGCCAGAGA 
AAAACGGAGG AGGAGGTGAC AGAACCTCAG CCGATGGTGT TTGGCCAGCA GCTGATGTTG 
ACCGCCTCAC CTGCTCCAGT GACCCCCCAG ACGGGCTACC CGAGCTATGC CTACCCCCCC 
GCAGGTTACC CTGCCGCCCC CGCTGCTGGC TACCCCCCTC AACCCCCAGC ATACGGCTTC 
AACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101077362 ( XM_029829714.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029685574.1 Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo60673
Clone ID Related Accession (Same CDS sequence)	XM_029829714.1
Accession Version	XM_029829714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	clathrin heavy chain 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042305.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG ACGCTGCCAA GACCCTGCAG ATCTTTAACA TTGAGATGAA AAGTAAACTA 
AAGGCTCACA CCATGACAGA AGATGTGATG TTCTGGAAGT GGATATCGGT GAACACCGTC 
GCCCTGGTGA CTGATTCAGC CGTCTATCAC TGGAGCATGG AGGGAGACTC TCAGCCCGCT 
AAGGTTTTCG ACCGGCACGC CAGTCTGGCA GGCTGTCAGA TCATCAACTA CAGAACTGAT 
CAGCAACAGA AGTGGCTGCT TTTGATCGGG ATCTCCGCGC AGCAAAACCG CGTGGTTGGA 
GCCATGCAGC TGTATTCAGT CGACAGGAAA GTGTCCCAGC CCATCGAGGG ACACGCTGCT 
GCCTTTGGAG ACTTCAAAGT GGAGGGGAAC ACCAAACCTT CCACCCTCTT TTGCTTCGCC 
GTGCGCTCGC AAGCTGGGGG AAAGTTGCAC ATCATTGAAG TCGGTCAGCC AGCTGCAGGG 
AACCAGCCCT TTGCTAAGAA AGCCGTTGAT GTGTTTTTCC CTCCAGAGGC GCAGACAGAC 
TTTCCCGTAG CCATGCAGAT CGGAAGTAAA CATGGAGTCA TATATTTGAT CACAAAGTAT 
GGTTACATTC ACTTGTACGA CCTGGAGTCT GGAGTTTGCA TCTACATGAA TCGAATTAGC 
GCGGAGACCA TTTTTGTCAC GGCTCCACAT GAAGCCACCT CAGGAATCAT TGGGGTGAAC 
AAGAAGGGTC AGGTCCTCTC AGTCTGCGTT GAAGAGGAAA ACATCGTCAA CTACGCCACC 
AACGTTTTAC AGAACCCAGA CCTGGCGTTG AGGATAGCTT TAAGGTCCAA TCTCGCTGGG 
GCGGAGGAGA TTTTTGCGAG GAAGTTCAAC ACACTGTTCT CCCAGGGAAA TTATTCAGAG 
GCTGCAAAGG TTGCTGCCTC GGCACCTAAG GGAATTCTCC GCACTGCAGA GACCATTCGT 
AAGTTCCAGA GCGTCCCAGC TCAGCCAGGT CAGGCCTCCC CATTGTTGCA GTACTTTGGT 
ATTCTGCTGG ACCAGGGTCA GCTCAACAAG TTTGAGTCTT TGGAGCTGTG CCGGCCTGTC 
CTGCAGCAGG GCCGCAAGCA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GTTGGAGTGC 
TCAGAAGAGC TGGGAGACCT GGTGAAGGCA TCGGATCCCA CTTTAGCTCT TAGCGTGTAC 
CTCAGAGCTA ACGTCCCCAA CAAGGTCATC CAGTGTTTTG CTGAAACTGG CCAGTTCCAG 
AAGATTGTGC TTTATGCCAA AAAGGTGGGC TACACCCCTG ACTGGGTTTT CCTCCTGAGA 
AACGTGATGC GTGTAAATCC AGACCAGGGC CTGCAGTTCG CCCAGATGCT TGTCCAGGAC 
GAGGAGCCGC TGGCCAACAT AAACCAGATT GTTGACGTCT TCATGGAGGG AAATCTGATC 
CAGCAGTGCA CGTCCTTCCT GTTGGACGCT CTGAAGAACA ACCGTCCCGC CGAAGGACAC 
TTACAGACTC GCCTGCTTGA GATGAACCTC ATACATGCCC CCCAGGTCGC GGATGCCATC 
CTTGGGAACC AGATGTTCAC CCATTATGAC CGGGCCCATG TGGCCCAGCT GTGCGAGAAG 
GCAGGTCTCC TGCAAAGAGC TCTTGAACAC TACACAGATC TGTACGACAT CAAACGGGCA 
GTGGTGCACA CGCATCTGCT CAACCCTGAG TGGCTGGTGA ACTTTTTTGG CTCGTTGTCA 
GTTGAAGACT CTTTGGAATG TCTGAGAGCC ATGTTGTCAG CTAACATCAG GCAGAACCTG 
CAGCTCTGCG TCCAGGTAGC ATCGAAGTAT CACGAGCAGC TGGGGACTCA GTCTTTAGTG 
GAACTCTTTG AATCCTTCAA GAGTTATGAG GGTTTGTTTT ACTTCCTTGG ATCGATTGTG 
AATTTCAGTC AGGAGCCTGA TGTTCACTTT AAGTACATCC AGGCTGCCTG CAAAACAGGT 
CAGATCAAGG AAGTGGAGAG AATCTGTCGA GAGAGCAACT GCTACGACCC AGAGAGAGTC 
AAAAACTTCC TCAAGGAGGC CAAGCTTACA GATCAGCTCC CTCTGATCAT CGTGTGTGAT 
CGCTTTGACT TTGTTCACGA CTTGGTGCTC TATCTCTACC GCAACAATCT CCAGAAATAC 
ATTGAAATCT ACGTGCAAAA GGTGAATCCC AGTCGTTTAC CGGTGGTTAT AGGTGGACTG 
TTGGATGTCG ACTGTGCTGA GGATGTGATC AAGAACCTGA TCATTGTGGT CAGAGGGCAG 
TTTTCCACTG ATGAGCTAGT AGAGGAAGTA GAAAAGAGGA ACAGGTTAAA GCTGTTGTTA 
CCATGGCTTG AGTCTCGCAT CCATGAAGGG TGCGAGGAAC CGGCCACTCA CAATGCGCTG 
GCCAAGATCT ATATTGATAG CAACAATACG CCCGAGCGTT TCCTGAAAGA GAACCCATTC 
TACGACAGCG CTGTTGTTGG AAAATACTGT GAGAAGAGAG ACCCTCACCT GGCCTGTGTA 
GCTTATGAGA GAGGACAATG TGACCTGGAC CTTATAAAGG TGTGCAATGA AAACTCCTTA 
TTCAAGAGTG AGGCTAGATA TCTGGTCCAT CGGAAAGACC CAGAGCTTTG GGCAAATGTG 
CTTGAAGAAA ACAACCCATT CAGACGACAA CTTATCGATC AGGTGGTGCA GACAGCCCTG 
TCAGAGACCC AGGACCCAGA GGAGGTTTCA GTAACCGTCA AGGCCTTCAT GACTGCAGAC 
CTTCCCAATG AGCTGATTGA ACTGCTGGAA AAGATCGTAC TCGACAACTC TGTCTTTAGC 
GAGCACAGAA ACCTCCAGAA CCTGCTCATT CTGACTGCCA TCAAGGCCGA CCGTACTCGG 
GTGATGGAGT ACATTAACAG ACTAGACAAC TACGACGCTC CGGACATTGC TAATATCGCC 
ATCAGCAATG AGCTATTTGA AGAAGCGTTT GCCATTTTCA AGAAGTTTGA TGTCAACACC 
TCTGCAATAC AGGTATTGAT AGAGCACATA GGGAACCTGG ACCGAGCCTA CGAGTTTGCA 
GAGCGCTGTA ACGAACCTGC GGTGTGGAGC CAGTTGGGCA GAGCTCAGCT GCACCGAGGC 
CTCGTCAAGG AGGCCATCGA CTCGTACATC AAAGCTGTCG ACCCTTCTGC GTACATGGAG 
GTGGTCAATG CAGCCAGCAA GAACAATAAT TGGGAAGATC TGGTGAAATT CCTCCAGATG 
GCTCGAAAGA AAGCCAGAGA GTCCTACGTG GAGACGGAGC TCATCTTCGC CTTGGCTAAA 
ACAAACCGTC TGGCCGAACT GGAGGAGTTC GTCAGTGGAC CCAACAATGC TCACATCCAG 
CAGGTGGGCG ATAGATGCTA CGAGGAAGGC ATGTACGAGG CAGCGAAGCT CTTGTATAAC 
AACGTTTCCA ACTTTGCTCG TCTCGCCTCG ACACTCGTAC ATCTCGGGGA GTACCAGGCG 
GCCGTGGACA GTGCCAGGAA AGCCAACAGC ACACGGACAT GGAAGGAGGT CTGCTTTGCG 
TGCGTGGATG GGGAGGAGTT CCGTCTGGCG CAAATCTGCG GCCTCCACAT CGTCATCCAC 
GCTGACGAGC TGGAAGACCT GATCAGCTAC TATCAGGACC GCGGATACTT CGAGGAACTC 
ATTGCGCTGC TGGAGGCGGC GCTGGGTTTG GAGCGGGCTC ACATGGGCAT GTTCACAGAG 
CTCGCCATCC TCTACTCCAA GTTCAAACCT CAGAAGATGA GGGAACACTT GGAGCTCTTC 
TGGTCCAGAG TCAATATTCC AAAGGTCCTC CGTGCAGCAG AGCAGTCTCA CCTCTGGGCA 
GAGCTGGTTT TCCTTTACGA TAAATACGAG GAGTACGACA ACGCAGTCCT CACGATGATG 
TCTCATCCGA CTGAAGCTTG GAAAGAAGGG CTGTTCAAAG ACATTATCCC AAAAGTGGCC 
AATGTGGAAC TGTACTATAA ATCCCTCTCC TTCTATCTGG ACTATAAACC GCTCCTACTC 
AACGACCTGC TGACCATCTT GTCTCCACGG CTTGATCACA GCCGAGCGGT CTCGTTTTTC 
TCAAAGATGA ACCAGCTGAA GCTGGTTAAG CCTTACCTGA GATCAGTCCA GAACCACAAC 
AACAAGTCTG TCAACGAGGC ACTCAACAAC CTGTTGACAG AGGAGGAAGA CTATCAGGGT 
CTGAGAGCGT CCATCGATGC CTACGACAAC TTTGATACCA TTGGCCTGGC GCAGAGATTA 
GAGAAGCACG ACCTGATCGA GTTCAGACGC ATTGCTGCTT ATCTTTATAA GGGTAACAAC 
CGCTGGAGAC AGAGTGTTGA GCTCTGCAAG AAGGACAAAC TCTACAAGGA TGCCATGCTG 
TACGCTGCAG AGTCGAAGGA TGCTGAGCTG GCAGAAAATC TGCTTCAGTG GTTCCTGGAG 
GAAGGCAGGA AAGAGTGCTT TGCTGCTTGC CTCTTTGCAT CCTATGACCT GCTGCATCCC 
GACGTAGTGC TGGAGCTGGC CTGGAGGCAC AATATCATGG ACTTCGCCAT GCCGTACTTC 
ATCCAGGTCA TGAGGGAGTA CCTCACAAAG GTCGATGCGT TTGCAGCAAA GGTGACGGTG 
GACAAACTGG AGGAGGCAGA GAGCCAGAGA AAAACGGAGG AGGAGGTGAC AGAACCTCAG 
CCGATGGTGT TTGGCCAGCA GCTGATGTTG ACCGCCTCAC CTGCTCCAGT GACCCCCCAG 
ACGGGCTACC CGAGCTATGC CTACCCCCCC GCAGGTTACC CTGCCGCCCC CGCTGCTGGC 
TACCCCCCTC AACCCCCAGC ATACGGCTTC AACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029685574.1
CDS	402..5480
Translation	MAQILPIRFQEHLQLQNMGVNPANIGFSYLTMESDKFICIREKVGDQNQVVIVDMSDPTNPIRRPISADSAIMNPASKVIALKDAAKTLQIFNIEMKSKLKAHTMTEDVMFWKWISVNTVALVTDSAVYHWSMEGDSQPAKVFDRHASLAGCQIINYRTDQQQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFGDFKVEGNTKPSTLFCFAVRSQAGGKLHIIEVGQPAAGNQPFAKKAVDVFFPPEAQTDFPVAMQIGSKHGVIYLITKYGYIHLYDLESGVCIYMNRISAETIFVTAPHEATSGIIGVNKKGQVLSVCVEEENIVNYATNVLQNPDLALRIALRSNLAGAEEIFARKFNTLFSQGNYSEAAKVAASAPKGILRTAETIRKFQSVPAQPGQASPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKASDPTLALSVYLRANVPNKVIQCFAETGQFQKIVLYAKKVGYTPDWVFLLRNVMRVNPDQGLQFAQMLVQDEEPLANINQIVDVFMEGNLIQQCTSFLLDALKNNRPAEGHLQTRLLEMNLIHAPQVADAILGNQMFTHYDRAHVAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVEDSLECLRAMLSANIRQNLQLCVQVASKYHEQLGTQSLVELFESFKSYEGLFYFLGSIVNFSQEPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCAEDVIKNLIIVVRGQFSTDELVEEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNTPERFLKENPFYDSAVVGKYCEKRDPHLACVAYERGQCDLDLIKVCNENSLFKSEARYLVHRKDPELWANVLEENNPFRRQLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFKKFDVNTSAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLGRAQLHRGLVKEAIDSYIKAVDPSAYMEVVNAASKNNNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFVSGPNNAHIQQVGDRCYEEGMYEAAKLLYNNVSNFARLASTLVHLGEYQAAVDSARKANSTRTWKEVCFACVDGEEFRLAQICGLHIVIHADELEDLISYYQDRGYFEELIALLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQSHLWAELVFLYDKYEEYDNAVLTMMSHPTEAWKEGLFKDIIPKVANVELYYKSLSFYLDYKPLLLNDLLTILSPRLDHSRAVSFFSKMNQLKLVKPYLRSVQNHNNKSVNEALNNLLTEEEDYQGLRASIDAYDNFDTIGLAQRLEKHDLIEFRRIAAYLYKGNNRWRQSVELCKKDKLYKDAMLYAAESKDAELAENLLQWFLEEGRKECFAACLFASYDLLHPDVVLELAWRHNIMDFAMPYFIQVMREYLTKVDAFAAKVTVDKLEEAESQRKTEEEVTEPQPMVFGQQLMLTASPAPVTPQTGYPSYAYPPAGYPAAPAAGYPPQPPAYGFNM

Target ORF information:

RefSeq Version	XM_029829714.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029829714.1

ORF Insert Sequence:

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG ACGCTGCCAA GACCCTGCAG ATCTTTAACA TTGAGATGAA AAGTAAACTA 
AAGGCTCACA CCATGACAGA AGATGTGATG TTCTGGAAGT GGATATCGGT GAACACCGTC 
GCCCTGGTGA CTGATTCAGC CGTCTATCAC TGGAGCATGG AGGGAGACTC TCAGCCCGCT 
AAGGTTTTCG ACCGGCACGC CAGTCTGGCA GGCTGTCAGA TCATCAACTA CAGAACTGAT 
CAGCAACAGA AGTGGCTGCT TTTGATCGGG ATCTCCGCGC AGCAAAACCG CGTGGTTGGA 
GCCATGCAGC TGTATTCAGT CGACAGGAAA GTGTCCCAGC CCATCGAGGG ACACGCTGCT 
GCCTTTGGAG ACTTCAAAGT GGAGGGGAAC ACCAAACCTT CCACCCTCTT TTGCTTCGCC 
GTGCGCTCGC AAGCTGGGGG AAAGTTGCAC ATCATTGAAG TCGGTCAGCC AGCTGCAGGG 
AACCAGCCCT TTGCTAAGAA AGCCGTTGAT GTGTTTTTCC CTCCAGAGGC GCAGACAGAC 
TTTCCCGTAG CCATGCAGAT CGGAAGTAAA CATGGAGTCA TATATTTGAT CACAAAGTAT 
GGTTACATTC ACTTGTACGA CCTGGAGTCT GGAGTTTGCA TCTACATGAA TCGAATTAGC 
GCGGAGACCA TTTTTGTCAC GGCTCCACAT GAAGCCACCT CAGGAATCAT TGGGGTGAAC 
AAGAAGGGTC AGGTCCTCTC AGTCTGCGTT GAAGAGGAAA ACATCGTCAA CTACGCCACC 
AACGTTTTAC AGAACCCAGA CCTGGCGTTG AGGATAGCTT TAAGGTCCAA TCTCGCTGGG 
GCGGAGGAGA TTTTTGCGAG GAAGTTCAAC ACACTGTTCT CCCAGGGAAA TTATTCAGAG 
GCTGCAAAGG TTGCTGCCTC GGCACCTAAG GGAATTCTCC GCACTGCAGA GACCATTCGT 
AAGTTCCAGA GCGTCCCAGC TCAGCCAGGT CAGGCCTCCC CATTGTTGCA GTACTTTGGT 
ATTCTGCTGG ACCAGGGTCA GCTCAACAAG TTTGAGTCTT TGGAGCTGTG CCGGCCTGTC 
CTGCAGCAGG GCCGCAAGCA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GTTGGAGTGC 
TCAGAAGAGC TGGGAGACCT GGTGAAGGCA TCGGATCCCA CTTTAGCTCT TAGCGTGTAC 
CTCAGAGCTA ACGTCCCCAA CAAGGTCATC CAGTGTTTTG CTGAAACTGG CCAGTTCCAG 
AAGATTGTGC TTTATGCCAA AAAGGTGGGC TACACCCCTG ACTGGGTTTT CCTCCTGAGA 
AACGTGATGC GTGTAAATCC AGACCAGGGC CTGCAGTTCG CCCAGATGCT TGTCCAGGAC 
GAGGAGCCGC TGGCCAACAT AAACCAGATT GTTGACGTCT TCATGGAGGG AAATCTGATC 
CAGCAGTGCA CGTCCTTCCT GTTGGACGCT CTGAAGAACA ACCGTCCCGC CGAAGGACAC 
TTACAGACTC GCCTGCTTGA GATGAACCTC ATACATGCCC CCCAGGTCGC GGATGCCATC 
CTTGGGAACC AGATGTTCAC CCATTATGAC CGGGCCCATG TGGCCCAGCT GTGCGAGAAG 
GCAGGTCTCC TGCAAAGAGC TCTTGAACAC TACACAGATC TGTACGACAT CAAACGGGCA 
GTGGTGCACA CGCATCTGCT CAACCCTGAG TGGCTGGTGA ACTTTTTTGG CTCGTTGTCA 
GTTGAAGACT CTTTGGAATG TCTGAGAGCC ATGTTGTCAG CTAACATCAG GCAGAACCTG 
CAGCTCTGCG TCCAGGTAGC ATCGAAGTAT CACGAGCAGC TGGGGACTCA GTCTTTAGTG 
GAACTCTTTG AATCCTTCAA GAGTTATGAG GGTTTGTTTT ACTTCCTTGG ATCGATTGTG 
AATTTCAGTC AGGAGCCTGA TGTTCACTTT AAGTACATCC AGGCTGCCTG CAAAACAGGT 
CAGATCAAGG AAGTGGAGAG AATCTGTCGA GAGAGCAACT GCTACGACCC AGAGAGAGTC 
AAAAACTTCC TCAAGGAGGC CAAGCTTACA GATCAGCTCC CTCTGATCAT CGTGTGTGAT 
CGCTTTGACT TTGTTCACGA CTTGGTGCTC TATCTCTACC GCAACAATCT CCAGAAATAC 
ATTGAAATCT ACGTGCAAAA GGTGAATCCC AGTCGTTTAC CGGTGGTTAT AGGTGGACTG 
TTGGATGTCG ACTGTGCTGA GGATGTGATC AAGAACCTGA TCATTGTGGT CAGAGGGCAG 
TTTTCCACTG ATGAGCTAGT AGAGGAAGTA GAAAAGAGGA ACAGGTTAAA GCTGTTGTTA 
CCATGGCTTG AGTCTCGCAT CCATGAAGGG TGCGAGGAAC CGGCCACTCA CAATGCGCTG 
GCCAAGATCT ATATTGATAG CAACAATACG CCCGAGCGTT TCCTGAAAGA GAACCCATTC 
TACGACAGCG CTGTTGTTGG AAAATACTGT GAGAAGAGAG ACCCTCACCT GGCCTGTGTA 
GCTTATGAGA GAGGACAATG TGACCTGGAC CTTATAAAGG TGTGCAATGA AAACTCCTTA 
TTCAAGAGTG AGGCTAGATA TCTGGTCCAT CGGAAAGACC CAGAGCTTTG GGCAAATGTG 
CTTGAAGAAA ACAACCCATT CAGACGACAA CTTATCGATC AGGTGGTGCA GACAGCCCTG 
TCAGAGACCC AGGACCCAGA GGAGGTTTCA GTAACCGTCA AGGCCTTCAT GACTGCAGAC 
CTTCCCAATG AGCTGATTGA ACTGCTGGAA AAGATCGTAC TCGACAACTC TGTCTTTAGC 
GAGCACAGAA ACCTCCAGAA CCTGCTCATT CTGACTGCCA TCAAGGCCGA CCGTACTCGG 
GTGATGGAGT ACATTAACAG ACTAGACAAC TACGACGCTC CGGACATTGC TAATATCGCC 
ATCAGCAATG AGCTATTTGA AGAAGCGTTT GCCATTTTCA AGAAGTTTGA TGTCAACACC 
TCTGCAATAC AGGTATTGAT AGAGCACATA GGGAACCTGG ACCGAGCCTA CGAGTTTGCA 
GAGCGCTGTA ACGAACCTGC GGTGTGGAGC CAGTTGGGCA GAGCTCAGCT GCACCGAGGC 
CTCGTCAAGG AGGCCATCGA CTCGTACATC AAAGCTGTCG ACCCTTCTGC GTACATGGAG 
GTGGTCAATG CAGCCAGCAA GAACAATAAT TGGGAAGATC TGGTGAAATT CCTCCAGATG 
GCTCGAAAGA AAGCCAGAGA GTCCTACGTG GAGACGGAGC TCATCTTCGC CTTGGCTAAA 
ACAAACCGTC TGGCCGAACT GGAGGAGTTC GTCAGTGGAC CCAACAATGC TCACATCCAG 
CAGGTGGGCG ATAGATGCTA CGAGGAAGGC ATGTACGAGG CAGCGAAGCT CTTGTATAAC 
AACGTTTCCA ACTTTGCTCG TCTCGCCTCG ACACTCGTAC ATCTCGGGGA GTACCAGGCG 
GCCGTGGACA GTGCCAGGAA AGCCAACAGC ACACGGACAT GGAAGGAGGT CTGCTTTGCG 
TGCGTGGATG GGGAGGAGTT CCGTCTGGCG CAAATCTGCG GCCTCCACAT CGTCATCCAC 
GCTGACGAGC TGGAAGACCT GATCAGCTAC TATCAGGACC GCGGATACTT CGAGGAACTC 
ATTGCGCTGC TGGAGGCGGC GCTGGGTTTG GAGCGGGCTC ACATGGGCAT GTTCACAGAG 
CTCGCCATCC TCTACTCCAA GTTCAAACCT CAGAAGATGA GGGAACACTT GGAGCTCTTC 
TGGTCCAGAG TCAATATTCC AAAGGTCCTC CGTGCAGCAG AGCAGTCTCA CCTCTGGGCA 
GAGCTGGTTT TCCTTTACGA TAAATACGAG GAGTACGACA ACGCAGTCCT CACGATGATG 
TCTCATCCGA CTGAAGCTTG GAAAGAAGGG CTGTTCAAAG ACATTATCCC AAAAGTGGCC 
AATGTGGAAC TGTACTATAA ATCCCTCTCC TTCTATCTGG ACTATAAACC GCTCCTACTC 
AACGACCTGC TGACCATCTT GTCTCCACGG CTTGATCACA GCCGAGCGGT CTCGTTTTTC 
TCAAAGATGA ACCAGCTGAA GCTGGTTAAG CCTTACCTGA GATCAGTCCA GAACCACAAC 
AACAAGTCTG TCAACGAGGC ACTCAACAAC CTGTTGACAG AGGAGGAAGA CTATCAGGGT 
CTGAGAGCGT CCATCGATGC CTACGACAAC TTTGATACCA TTGGCCTGGC GCAGAGATTA 
GAGAAGCACG ACCTGATCGA GTTCAGACGC ATTGCTGCTT ATCTTTATAA GGGTAACAAC 
CGCTGGAGAC AGAGTGTTGA GCTCTGCAAG AAGGACAAAC TCTACAAGGA TGCCATGCTG 
TACGCTGCAG AGTCGAAGGA TGCTGAGCTG GCAGAAAATC TGCTTCAGTG GTTCCTGGAG 
GAAGGCAGGA AAGAGTGCTT TGCTGCTTGC CTCTTTGCAT CCTATGACCT GCTGCATCCC 
GACGTAGTGC TGGAGCTGGC CTGGAGGCAC AATATCATGG ACTTCGCCAT GCCGTACTTC 
ATCCAGGTCA TGAGGGAGTA CCTCACAAAG GTCGATGCGT TTGCAGCAAA GGTGACGGTG 
GACAAACTGG AGGAGGCAGA GAGCCAGAGA AAAACGGAGG AGGAGGTGAC AGAACCTCAG 
CCGATGGTGT TTGGCCAGCA GCTGATGTTG ACCGCCTCAC CTGCTCCAGT GACCCCCCAG 
ACGGGCTACC CGAGCTATGC CTACCCCCCC GCAGGTTACC CTGCCGCCCC CGCTGCTGGC 
TACCCCCCTC AACCCCCAGC ATACGGCTTC AACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101077362 ( XM_029829715.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029685575.1 Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X2, mRNA.

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CloneID	OTo60674
Clone ID Related Accession (Same CDS sequence)	XM_029829715.1
Accession Version	XM_029829715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	clathrin heavy chain 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042305.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG CTGCCAAGAC CCTGCAGATC TTTAACATTG AGATGAAAAG TAAACTAAAG 
GCTCACACCA TGACAGAAGA TGTGATGTTC TGGAAGTGGA TATCGGTGAA CACCGTCGCC 
CTGGTGACTG ATTCAGCCGT CTATCACTGG AGCATGGAGG GAGACTCTCA GCCCGCTAAG 
GTTTTCGACC GGCACGCCAG TCTGGCAGGC TGTCAGATCA TCAACTACAG AACTGATCAG 
CAACAGAAGT GGCTGCTTTT GATCGGGATC TCCGCGCAGC AAAACCGCGT GGTTGGAGCC 
ATGCAGCTGT ATTCAGTCGA CAGGAAAGTG TCCCAGCCCA TCGAGGGACA CGCTGCTGCC 
TTTGGAGACT TCAAAGTGGA GGGGAACACC AAACCTTCCA CCCTCTTTTG CTTCGCCGTG 
CGCTCGCAAG CTGGGGGAAA GTTGCACATC ATTGAAGTCG GTCAGCCAGC TGCAGGGAAC 
CAGCCCTTTG CTAAGAAAGC CGTTGATGTG TTTTTCCCTC CAGAGGCGCA GACAGACTTT 
CCCGTAGCCA TGCAGATCGG AAGTAAACAT GGAGTCATAT ATTTGATCAC AAAGTATGGT 
TACATTCACT TGTACGACCT GGAGTCTGGA GTTTGCATCT ACATGAATCG AATTAGCGCG 
GAGACCATTT TTGTCACGGC TCCACATGAA GCCACCTCAG GAATCATTGG GGTGAACAAG 
AAGGGTCAGG TCCTCTCAGT CTGCGTTGAA GAGGAAAACA TCGTCAACTA CGCCACCAAC 
GTTTTACAGA ACCCAGACCT GGCGTTGAGG ATAGCTTTAA GGTCCAATCT CGCTGGGGCG 
GAGGAGATTT TTGCGAGGAA GTTCAACACA CTGTTCTCCC AGGGAAATTA TTCAGAGGCT 
GCAAAGGTTG CTGCCTCGGC ACCTAAGGGA ATTCTCCGCA CTGCAGAGAC CATTCGTAAG 
TTCCAGAGCG TCCCAGCTCA GCCAGGTCAG GCCTCCCCAT TGTTGCAGTA CTTTGGTATT 
CTGCTGGACC AGGGTCAGCT CAACAAGTTT GAGTCTTTGG AGCTGTGCCG GCCTGTCCTG 
CAGCAGGGCC GCAAGCAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGTT GGAGTGCTCA 
GAAGAGCTGG GAGACCTGGT GAAGGCATCG GATCCCACTT TAGCTCTTAG CGTGTACCTC 
AGAGCTAACG TCCCCAACAA GGTCATCCAG TGTTTTGCTG AAACTGGCCA GTTCCAGAAG 
ATTGTGCTTT ATGCCAAAAA GGTGGGCTAC ACCCCTGACT GGGTTTTCCT CCTGAGAAAC 
GTGATGCGTG TAAATCCAGA CCAGGGCCTG CAGTTCGCCC AGATGCTTGT CCAGGACGAG 
GAGCCGCTGG CCAACATAAA CCAGATTGTT GACGTCTTCA TGGAGGGAAA TCTGATCCAG 
CAGTGCACGT CCTTCCTGTT GGACGCTCTG AAGAACAACC GTCCCGCCGA AGGACACTTA 
CAGACTCGCC TGCTTGAGAT GAACCTCATA CATGCCCCCC AGGTCGCGGA TGCCATCCTT 
GGGAACCAGA TGTTCACCCA TTATGACCGG GCCCATGTGG CCCAGCTGTG CGAGAAGGCA 
GGTCTCCTGC AAAGAGCTCT TGAACACTAC ACAGATCTGT ACGACATCAA ACGGGCAGTG 
GTGCACACGC ATCTGCTCAA CCCTGAGTGG CTGGTGAACT TTTTTGGCTC GTTGTCAGTT 
GAAGACTCTT TGGAATGTCT GAGAGCCATG TTGTCAGCTA ACATCAGGCA GAACCTGCAG 
CTCTGCGTCC AGGTAGCATC GAAGTATCAC GAGCAGCTGG GGACTCAGTC TTTAGTGGAA 
CTCTTTGAAT CCTTCAAGAG TTATGAGGGT TTGTTTTACT TCCTTGGATC GATTGTGAAT 
TTCAGTCAGG AGCCTGATGT TCACTTTAAG TACATCCAGG CTGCCTGCAA AACAGGTCAG 
ATCAAGGAAG TGGAGAGAAT CTGTCGAGAG AGCAACTGCT ACGACCCAGA GAGAGTCAAA 
AACTTCCTCA AGGAGGCCAA GCTTACAGAT CAGCTCCCTC TGATCATCGT GTGTGATCGC 
TTTGACTTTG TTCACGACTT GGTGCTCTAT CTCTACCGCA ACAATCTCCA GAAATACATT 
GAAATCTACG TGCAAAAGGT GAATCCCAGT CGTTTACCGG TGGTTATAGG TGGACTGTTG 
GATGTCGACT GTGCTGAGGA TGTGATCAAG AACCTGATCA TTGTGGTCAG AGGGCAGTTT 
TCCACTGATG AGCTAGTAGA GGAAGTAGAA AAGAGGAACA GGTTAAAGCT GTTGTTACCA 
TGGCTTGAGT CTCGCATCCA TGAAGGGTGC GAGGAACCGG CCACTCACAA TGCGCTGGCC 
AAGATCTATA TTGATAGCAA CAATACGCCC GAGCGTTTCC TGAAAGAGAA CCCATTCTAC 
GACAGCGCTG TTGTTGGAAA ATACTGTGAG AAGAGAGACC CTCACCTGGC CTGTGTAGCT 
TATGAGAGAG GACAATGTGA CCTGGACCTT ATAAAGGTGT GCAATGAAAA CTCCTTATTC 
AAGAGTGAGG CTAGATATCT GGTCCATCGG AAAGACCCAG AGCTTTGGGC AAATGTGCTT 
GAAGAAAACA ACCCATTCAG ACGACAACTT ATCGATCAGG TGGTGCAGAC AGCCCTGTCA 
GAGACCCAGG ACCCAGAGGA GGTTTCAGTA ACCGTCAAGG CCTTCATGAC TGCAGACCTT 
CCCAATGAGC TGATTGAACT GCTGGAAAAG ATCGTACTCG ACAACTCTGT CTTTAGCGAG 
CACAGAAACC TCCAGAACCT GCTCATTCTG ACTGCCATCA AGGCCGACCG TACTCGGGTG 
ATGGAGTACA TTAACAGACT AGACAACTAC GACGCTCCGG ACATTGCTAA TATCGCCATC 
AGCAATGAGC TATTTGAAGA AGCGTTTGCC ATTTTCAAGA AGTTTGATGT CAACACCTCT 
GCAATACAGG TATTGATAGA GCACATAGGG AACCTGGACC GAGCCTACGA GTTTGCAGAG 
CGCTGTAACG AACCTGCGGT GTGGAGCCAG TTGGGCAGAG CTCAGCTGCA CCGAGGCCTC 
GTCAAGGAGG CCATCGACTC GTACATCAAA GCTGTCGACC CTTCTGCGTA CATGGAGGTG 
GTCAATGCAG CCAGCAAGAA CAATAATTGG GAAGATCTGG TGAAATTCCT CCAGATGGCT 
CGAAAGAAAG CCAGAGAGTC CTACGTGGAG ACGGAGCTCA TCTTCGCCTT GGCTAAAACA 
AACCGTCTGG CCGAACTGGA GGAGTTCGTC AGTGGACCCA ACAATGCTCA CATCCAGCAG 
GTGGGCGATA GATGCTACGA GGAAGGCATG TACGAGGCAG CGAAGCTCTT GTATAACAAC 
GTTTCCAACT TTGCTCGTCT CGCCTCGACA CTCGTACATC TCGGGGAGTA CCAGGCGGCC 
GTGGACAGTG CCAGGAAAGC CAACAGCACA CGGACATGGA AGGAGGTCTG CTTTGCGTGC 
GTGGATGGGG AGGAGTTCCG TCTGGCGCAA ATCTGCGGCC TCCACATCGT CATCCACGCT 
GACGAGCTGG AAGACCTGAT CAGCTACTAT CAGGACCGCG GATACTTCGA GGAACTCATT 
GCGCTGCTGG AGGCGGCGCT GGGTTTGGAG CGGGCTCACA TGGGCATGTT CACAGAGCTC 
GCCATCCTCT ACTCCAAGTT CAAACCTCAG AAGATGAGGG AACACTTGGA GCTCTTCTGG 
TCCAGAGTCA ATATTCCAAA GGTCCTCCGT GCAGCAGAGC AGTCTCACCT CTGGGCAGAG 
CTGGTTTTCC TTTACGATAA ATACGAGGAG TACGACAACG CAGTCCTCAC GATGATGTCT 
CATCCGACTG AAGCTTGGAA AGAAGGGCTG TTCAAAGACA TTATCCCAAA AGTGGCCAAT 
GTGGAACTGT ACTATAAATC CCTCTCCTTC TATCTGGACT ATAAACCGCT CCTACTCAAC 
GACCTGCTGA CCATCTTGTC TCCACGGCTT GATCACAGCC GAGCGGTCTC GTTTTTCTCA 
AAGATGAACC AGCTGAAGCT GGTTAAGCCT TACCTGAGAT CAGTCCAGAA CCACAACAAC 
AAGTCTGTCA ACGAGGCACT CAACAACCTG TTGACAGAGG AGGAAGACTA TCAGGGTCTG 
AGAGCGTCCA TCGATGCCTA CGACAACTTT GATACCATTG GCCTGGCGCA GAGATTAGAG 
AAGCACGACC TGATCGAGTT CAGACGCATT GCTGCTTATC TTTATAAGGG TAACAACCGC 
TGGAGACAGA GTGTTGAGCT CTGCAAGAAG GACAAACTCT ACAAGGATGC CATGCTGTAC 
GCTGCAGAGT CGAAGGATGC TGAGCTGGCA GAAAATCTGC TTCAGTGGTT CCTGGAGGAA 
GGCAGGAAAG AGTGCTTTGC TGCTTGCCTC TTTGCATCCT ATGACCTGCT GCATCCCGAC 
GTAGTGCTGG AGCTGGCCTG GAGGCACAAT ATCATGGACT TCGCCATGCC GTACTTCATC 
CAGGTCATGA GGGAGTACCT CACAAAGGTC GATGCGTTTG CAGCAAAGGT GACGGTGGAC 
AAACTGGAGG AGGCAGAGAG CCAGAGAAAA ACGGAGGAGG AGGTGACAGA ACCTCAGCCG 
ATGGTGTTTG GCCAGCAGCT GATGTTGACC GCCTCACCTG CTCCAGTGAC CCCCCAGACG 
GGCTACCCGA GCTATGCCTA CCCCCCCGCA GGTTACCCTG CCGCCCCCGC TGCTGGCTAC 
CCCCCTCAAC CCCCAGCATA CGGCTTCAAC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029685575.1
CDS	402..5477
Translation	MAQILPIRFQEHLQLQNMGVNPANIGFSYLTMESDKFICIREKVGDQNQVVIVDMSDPTNPIRRPISADSAIMNPASKVIALKAAKTLQIFNIEMKSKLKAHTMTEDVMFWKWISVNTVALVTDSAVYHWSMEGDSQPAKVFDRHASLAGCQIINYRTDQQQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFGDFKVEGNTKPSTLFCFAVRSQAGGKLHIIEVGQPAAGNQPFAKKAVDVFFPPEAQTDFPVAMQIGSKHGVIYLITKYGYIHLYDLESGVCIYMNRISAETIFVTAPHEATSGIIGVNKKGQVLSVCVEEENIVNYATNVLQNPDLALRIALRSNLAGAEEIFARKFNTLFSQGNYSEAAKVAASAPKGILRTAETIRKFQSVPAQPGQASPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKASDPTLALSVYLRANVPNKVIQCFAETGQFQKIVLYAKKVGYTPDWVFLLRNVMRVNPDQGLQFAQMLVQDEEPLANINQIVDVFMEGNLIQQCTSFLLDALKNNRPAEGHLQTRLLEMNLIHAPQVADAILGNQMFTHYDRAHVAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVEDSLECLRAMLSANIRQNLQLCVQVASKYHEQLGTQSLVELFESFKSYEGLFYFLGSIVNFSQEPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCAEDVIKNLIIVVRGQFSTDELVEEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNTPERFLKENPFYDSAVVGKYCEKRDPHLACVAYERGQCDLDLIKVCNENSLFKSEARYLVHRKDPELWANVLEENNPFRRQLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFKKFDVNTSAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLGRAQLHRGLVKEAIDSYIKAVDPSAYMEVVNAASKNNNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFVSGPNNAHIQQVGDRCYEEGMYEAAKLLYNNVSNFARLASTLVHLGEYQAAVDSARKANSTRTWKEVCFACVDGEEFRLAQICGLHIVIHADELEDLISYYQDRGYFEELIALLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQSHLWAELVFLYDKYEEYDNAVLTMMSHPTEAWKEGLFKDIIPKVANVELYYKSLSFYLDYKPLLLNDLLTILSPRLDHSRAVSFFSKMNQLKLVKPYLRSVQNHNNKSVNEALNNLLTEEEDYQGLRASIDAYDNFDTIGLAQRLEKHDLIEFRRIAAYLYKGNNRWRQSVELCKKDKLYKDAMLYAAESKDAELAENLLQWFLEEGRKECFAACLFASYDLLHPDVVLELAWRHNIMDFAMPYFIQVMREYLTKVDAFAAKVTVDKLEEAESQRKTEEEVTEPQPMVFGQQLMLTASPAPVTPQTGYPSYAYPPAGYPAAPAAGYPPQPPAYGFNM

Target ORF information:

RefSeq Version	XM_029829715.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029829715.1

ORF Insert Sequence:

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG CTGCCAAGAC CCTGCAGATC TTTAACATTG AGATGAAAAG TAAACTAAAG 
GCTCACACCA TGACAGAAGA TGTGATGTTC TGGAAGTGGA TATCGGTGAA CACCGTCGCC 
CTGGTGACTG ATTCAGCCGT CTATCACTGG AGCATGGAGG GAGACTCTCA GCCCGCTAAG 
GTTTTCGACC GGCACGCCAG TCTGGCAGGC TGTCAGATCA TCAACTACAG AACTGATCAG 
CAACAGAAGT GGCTGCTTTT GATCGGGATC TCCGCGCAGC AAAACCGCGT GGTTGGAGCC 
ATGCAGCTGT ATTCAGTCGA CAGGAAAGTG TCCCAGCCCA TCGAGGGACA CGCTGCTGCC 
TTTGGAGACT TCAAAGTGGA GGGGAACACC AAACCTTCCA CCCTCTTTTG CTTCGCCGTG 
CGCTCGCAAG CTGGGGGAAA GTTGCACATC ATTGAAGTCG GTCAGCCAGC TGCAGGGAAC 
CAGCCCTTTG CTAAGAAAGC CGTTGATGTG TTTTTCCCTC CAGAGGCGCA GACAGACTTT 
CCCGTAGCCA TGCAGATCGG AAGTAAACAT GGAGTCATAT ATTTGATCAC AAAGTATGGT 
TACATTCACT TGTACGACCT GGAGTCTGGA GTTTGCATCT ACATGAATCG AATTAGCGCG 
GAGACCATTT TTGTCACGGC TCCACATGAA GCCACCTCAG GAATCATTGG GGTGAACAAG 
AAGGGTCAGG TCCTCTCAGT CTGCGTTGAA GAGGAAAACA TCGTCAACTA CGCCACCAAC 
GTTTTACAGA ACCCAGACCT GGCGTTGAGG ATAGCTTTAA GGTCCAATCT CGCTGGGGCG 
GAGGAGATTT TTGCGAGGAA GTTCAACACA CTGTTCTCCC AGGGAAATTA TTCAGAGGCT 
GCAAAGGTTG CTGCCTCGGC ACCTAAGGGA ATTCTCCGCA CTGCAGAGAC CATTCGTAAG 
TTCCAGAGCG TCCCAGCTCA GCCAGGTCAG GCCTCCCCAT TGTTGCAGTA CTTTGGTATT 
CTGCTGGACC AGGGTCAGCT CAACAAGTTT GAGTCTTTGG AGCTGTGCCG GCCTGTCCTG 
CAGCAGGGCC GCAAGCAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGTT GGAGTGCTCA 
GAAGAGCTGG GAGACCTGGT GAAGGCATCG GATCCCACTT TAGCTCTTAG CGTGTACCTC 
AGAGCTAACG TCCCCAACAA GGTCATCCAG TGTTTTGCTG AAACTGGCCA GTTCCAGAAG 
ATTGTGCTTT ATGCCAAAAA GGTGGGCTAC ACCCCTGACT GGGTTTTCCT CCTGAGAAAC 
GTGATGCGTG TAAATCCAGA CCAGGGCCTG CAGTTCGCCC AGATGCTTGT CCAGGACGAG 
GAGCCGCTGG CCAACATAAA CCAGATTGTT GACGTCTTCA TGGAGGGAAA TCTGATCCAG 
CAGTGCACGT CCTTCCTGTT GGACGCTCTG AAGAACAACC GTCCCGCCGA AGGACACTTA 
CAGACTCGCC TGCTTGAGAT GAACCTCATA CATGCCCCCC AGGTCGCGGA TGCCATCCTT 
GGGAACCAGA TGTTCACCCA TTATGACCGG GCCCATGTGG CCCAGCTGTG CGAGAAGGCA 
GGTCTCCTGC AAAGAGCTCT TGAACACTAC ACAGATCTGT ACGACATCAA ACGGGCAGTG 
GTGCACACGC ATCTGCTCAA CCCTGAGTGG CTGGTGAACT TTTTTGGCTC GTTGTCAGTT 
GAAGACTCTT TGGAATGTCT GAGAGCCATG TTGTCAGCTA ACATCAGGCA GAACCTGCAG 
CTCTGCGTCC AGGTAGCATC GAAGTATCAC GAGCAGCTGG GGACTCAGTC TTTAGTGGAA 
CTCTTTGAAT CCTTCAAGAG TTATGAGGGT TTGTTTTACT TCCTTGGATC GATTGTGAAT 
TTCAGTCAGG AGCCTGATGT TCACTTTAAG TACATCCAGG CTGCCTGCAA AACAGGTCAG 
ATCAAGGAAG TGGAGAGAAT CTGTCGAGAG AGCAACTGCT ACGACCCAGA GAGAGTCAAA 
AACTTCCTCA AGGAGGCCAA GCTTACAGAT CAGCTCCCTC TGATCATCGT GTGTGATCGC 
TTTGACTTTG TTCACGACTT GGTGCTCTAT CTCTACCGCA ACAATCTCCA GAAATACATT 
GAAATCTACG TGCAAAAGGT GAATCCCAGT CGTTTACCGG TGGTTATAGG TGGACTGTTG 
GATGTCGACT GTGCTGAGGA TGTGATCAAG AACCTGATCA TTGTGGTCAG AGGGCAGTTT 
TCCACTGATG AGCTAGTAGA GGAAGTAGAA AAGAGGAACA GGTTAAAGCT GTTGTTACCA 
TGGCTTGAGT CTCGCATCCA TGAAGGGTGC GAGGAACCGG CCACTCACAA TGCGCTGGCC 
AAGATCTATA TTGATAGCAA CAATACGCCC GAGCGTTTCC TGAAAGAGAA CCCATTCTAC 
GACAGCGCTG TTGTTGGAAA ATACTGTGAG AAGAGAGACC CTCACCTGGC CTGTGTAGCT 
TATGAGAGAG GACAATGTGA CCTGGACCTT ATAAAGGTGT GCAATGAAAA CTCCTTATTC 
AAGAGTGAGG CTAGATATCT GGTCCATCGG AAAGACCCAG AGCTTTGGGC AAATGTGCTT 
GAAGAAAACA ACCCATTCAG ACGACAACTT ATCGATCAGG TGGTGCAGAC AGCCCTGTCA 
GAGACCCAGG ACCCAGAGGA GGTTTCAGTA ACCGTCAAGG CCTTCATGAC TGCAGACCTT 
CCCAATGAGC TGATTGAACT GCTGGAAAAG ATCGTACTCG ACAACTCTGT CTTTAGCGAG 
CACAGAAACC TCCAGAACCT GCTCATTCTG ACTGCCATCA AGGCCGACCG TACTCGGGTG 
ATGGAGTACA TTAACAGACT AGACAACTAC GACGCTCCGG ACATTGCTAA TATCGCCATC 
AGCAATGAGC TATTTGAAGA AGCGTTTGCC ATTTTCAAGA AGTTTGATGT CAACACCTCT 
GCAATACAGG TATTGATAGA GCACATAGGG AACCTGGACC GAGCCTACGA GTTTGCAGAG 
CGCTGTAACG AACCTGCGGT GTGGAGCCAG TTGGGCAGAG CTCAGCTGCA CCGAGGCCTC 
GTCAAGGAGG CCATCGACTC GTACATCAAA GCTGTCGACC CTTCTGCGTA CATGGAGGTG 
GTCAATGCAG CCAGCAAGAA CAATAATTGG GAAGATCTGG TGAAATTCCT CCAGATGGCT 
CGAAAGAAAG CCAGAGAGTC CTACGTGGAG ACGGAGCTCA TCTTCGCCTT GGCTAAAACA 
AACCGTCTGG CCGAACTGGA GGAGTTCGTC AGTGGACCCA ACAATGCTCA CATCCAGCAG 
GTGGGCGATA GATGCTACGA GGAAGGCATG TACGAGGCAG CGAAGCTCTT GTATAACAAC 
GTTTCCAACT TTGCTCGTCT CGCCTCGACA CTCGTACATC TCGGGGAGTA CCAGGCGGCC 
GTGGACAGTG CCAGGAAAGC CAACAGCACA CGGACATGGA AGGAGGTCTG CTTTGCGTGC 
GTGGATGGGG AGGAGTTCCG TCTGGCGCAA ATCTGCGGCC TCCACATCGT CATCCACGCT 
GACGAGCTGG AAGACCTGAT CAGCTACTAT CAGGACCGCG GATACTTCGA GGAACTCATT 
GCGCTGCTGG AGGCGGCGCT GGGTTTGGAG CGGGCTCACA TGGGCATGTT CACAGAGCTC 
GCCATCCTCT ACTCCAAGTT CAAACCTCAG AAGATGAGGG AACACTTGGA GCTCTTCTGG 
TCCAGAGTCA ATATTCCAAA GGTCCTCCGT GCAGCAGAGC AGTCTCACCT CTGGGCAGAG 
CTGGTTTTCC TTTACGATAA ATACGAGGAG TACGACAACG CAGTCCTCAC GATGATGTCT 
CATCCGACTG AAGCTTGGAA AGAAGGGCTG TTCAAAGACA TTATCCCAAA AGTGGCCAAT 
GTGGAACTGT ACTATAAATC CCTCTCCTTC TATCTGGACT ATAAACCGCT CCTACTCAAC 
GACCTGCTGA CCATCTTGTC TCCACGGCTT GATCACAGCC GAGCGGTCTC GTTTTTCTCA 
AAGATGAACC AGCTGAAGCT GGTTAAGCCT TACCTGAGAT CAGTCCAGAA CCACAACAAC 
AAGTCTGTCA ACGAGGCACT CAACAACCTG TTGACAGAGG AGGAAGACTA TCAGGGTCTG 
AGAGCGTCCA TCGATGCCTA CGACAACTTT GATACCATTG GCCTGGCGCA GAGATTAGAG 
AAGCACGACC TGATCGAGTT CAGACGCATT GCTGCTTATC TTTATAAGGG TAACAACCGC 
TGGAGACAGA GTGTTGAGCT CTGCAAGAAG GACAAACTCT ACAAGGATGC CATGCTGTAC 
GCTGCAGAGT CGAAGGATGC TGAGCTGGCA GAAAATCTGC TTCAGTGGTT CCTGGAGGAA 
GGCAGGAAAG AGTGCTTTGC TGCTTGCCTC TTTGCATCCT ATGACCTGCT GCATCCCGAC 
GTAGTGCTGG AGCTGGCCTG GAGGCACAAT ATCATGGACT TCGCCATGCC GTACTTCATC 
CAGGTCATGA GGGAGTACCT CACAAAGGTC GATGCGTTTG CAGCAAAGGT GACGGTGGAC 
AAACTGGAGG AGGCAGAGAG CCAGAGAAAA ACGGAGGAGG AGGTGACAGA ACCTCAGCCG 
ATGGTGTTTG GCCAGCAGCT GATGTTGACC GCCTCACCTG CTCCAGTGAC CCCCCAGACG 
GGCTACCCGA GCTATGCCTA CCCCCCCGCA GGTTACCCTG CCGCCCCCGC TGCTGGCTAC 
CCCCCTCAAC CCCCAGCATA CGGCTTCAAC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101077362 ( XM_029829716.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029685576.1 Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X3, mRNA.

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CloneID	OTo60675
Clone ID Related Accession (Same CDS sequence)	XM_029829716.1
Accession Version	XM_029829716.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	clathrin heavy chain 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042305.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG ACGCTGCCAA GACCCTGCAG ATCTTTAACA TTGAGATGAA AAGTAAACTA 
AAGGCTCACA CCATGACAGA AGATGTGATG TTCTGGAAGT GGATATCGGT GAACACCGTC 
GCCCTGGTGA CTGATTCAGC CGTCTATCAC TGGAGCATGG AGGGAGACTC TCAGCCCGCT 
AAGGTTTTCG ACCGGCACGC CAGTCTGGCA GGCTGTCAGA TCATCAACTA CAGAACTGAT 
CAGCAACAGA AGTGGCTGCT TTTGATCGGG ATCTCCGCGC AGCAAAACCG CGTGGTTGGA 
GCCATGCAGC TGTATTCAGT CGACAGGAAA GTGTCCCAGC CCATCGAGGG ACACGCTGCT 
GCCTTTGGAG ACTTCAAAGT GGAGGGGAAC ACCAAACCTT CCACCCTCTT TTGCTTCGCC 
GTGCGCTCGC AAGCTGGGGG AAAGTTGCAC ATCATTGAAG TCGGTCAGCC AGCTGCAGGG 
AACCAGCCCT TTGCTAAGAA AGCCGTTGAT GTGTTTTTCC CTCCAGAGGC GCAGACAGAC 
TTTCCCGTAG CCATGCAGAT CGGAAGTAAA CATGGAGTCA TATATTTGAT CACAAAGTAT 
GGTTACATTC ACTTGTACGA CCTGGAGTCT GGAGTTTGCA TCTACATGAA TCGAATTAGC 
GCGGAGACCA TTTTTGTCAC GGCTCCACAT GAAGCCACCT CAGGAATCAT TGGGGTGAAC 
AAGAAGGGTC AGGTCCTCTC AGTCTGCGTT GAAGAGGAAA ACATCGTCAA CTACGCCACC 
AACGTTTTAC AGAACCCAGA CCTGGCGTTG AGGATAGCTT TAAGGTCCAA TCTCGCTGGG 
GCGGAGGAGA TTTTTGCGAG GAAGTTCAAC ACACTGTTCT CCCAGGGAAA TTATTCAGAG 
GCTGCAAAGG TTGCTGCCTC GGCACCTAAG GGAATTCTCC GCACTGCAGA GACCATTCGT 
AAGTTCCAGA GCGTCCCAGC TCAGCCAGGT CAGGCCTCCC CATTGTTGCA GTACTTTGGT 
ATTCTGCTGG ACCAGGGTCA GCTCAACAAG TTTGAGTCTT TGGAGCTGTG CCGGCCTGTC 
CTGCAGCAGG GCCGCAAGCA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GTTGGAGTGC 
TCAGAAGAGC TGGGAGACCT GGTGAAGGCA TCGGATCCCA CTTTAGCTCT TAGCGTGTAC 
CTCAGAGCTA ACGTCCCCAA CAAGGTCATC CAGTGTTTTG CTGAAACTGG CCAGTTCCAG 
AAGATTGTGC TTTATGCCAA AAAGGTGGGC TACACCCCTG ACTGGGTTTT CCTCCTGAGA 
AACGTGATGC GTGTAAATCC AGACCAGGGC CTGCAGTTCG CCCAGATGCT TGTCCAGGAC 
GAGGAGCCGC TGGCCAACAT AAACCAGATT GTTGACGTCT TCATGGAGGG AAATCTGATC 
CAGCAGTGCA CGTCCTTCCT GTTGGACGCT CTGAAGAACA ACCGTCCCGC CGAAGGACAC 
TTACAGACTC GCCTGCTTGA GATGAACCTC ATACATGCCC CCCAGGTCGC GGATGCCATC 
CTTGGGAACC AGATGTTCAC CCATTATGAC CGGGCCCATG TGGCCCAGCT GTGCGAGAAG 
GCAGGTCTCC TGCAAAGAGC TCTTGAACAC TACACAGATC TGTACGACAT CAAACGGGCA 
GTGGTGCACA CGCATCTGCT CAACCCTGAG TGGCTGGTGA ACTTTTTTGG CTCGTTGTCA 
GTTGAAGACT CTTTGGAATG TCTGAGAGCC ATGTTGTCAG CTAACATCAG GCAGAACCTG 
CAGCTCTGCG TCCAGGTAGC ATCGAAGTAT CACGAGCAGC TGGGGACTCA GTCTTTAGTG 
GAACTCTTTG AATCCTTCAA GAGTTATGAG GGTTTGTTTT ACTTCCTTGG ATCGATTGTG 
AATTTCAGTC AGGAGCCTGA TGTTCACTTT AAGTACATCC AGGCTGCCTG CAAAACAGGT 
CAGATCAAGG AAGTGGAGAG AATCTGTCGA GAGAGCAACT GCTACGACCC AGAGAGAGTC 
AAAAACTTCC TCAAGGAGGC CAAGCTTACA GATCAGCTCC CTCTGATCAT CGTGTGTGAT 
CGCTTTGACT TTGTTCACGA CTTGGTGCTC TATCTCTACC GCAACAATCT CCAGAAATAC 
ATTGAAATCT ACGTGCAAAA GGTGAATCCC AGTCGTTTAC CGGTGGTTAT AGGTGGACTG 
TTGGATGTCG ACTGTGCTGA GGATGTGATC AAGAACCTGA TCATTGTGGT CAGAGGGCAG 
TTTTCCACTG ATGAGCTAGT AGAGGAAGTA GAAAAGAGGA ACAGGTTAAA GCTGTTGTTA 
CCATGGCTTG AGTCTCGCAT CCATGAAGGG TGCGAGGAAC CGGCCACTCA CAATGCGCTG 
GCCAAGATCT ATATTGATAG CAACAATACG CCCGAGCGTT TCCTGAAAGA GAACCCATTC 
TACGACAGCG CTGTTGTTGG AAAATACTGT GAGAAGAGAG ACCCTCACCT GGCCTGTGTA 
GCTTATGAGA GAGGACAATG TGACCTGGAC CTTATAAAGG TGTGCAATGA AAACTCCTTA 
TTCAAGAGTG AGGCTAGATA TCTGGTCCAT CGGAAAGACC CAGAGCTTTG GGCAAATGTG 
CTTGAAGAAA ACAACCCATT CAGACGACAA CTTATCGATC AGGTGGTGCA GACAGCCCTG 
TCAGAGACCC AGGACCCAGA GGAGGTTTCA GTAACCGTCA AGGCCTTCAT GACTGCAGAC 
CTTCCCAATG AGCTGATTGA ACTGCTGGAA AAGATCGTAC TCGACAACTC TGTCTTTAGC 
GAGCACAGAA ACCTCCAGAA CCTGCTCATT CTGACTGCCA TCAAGGCCGA CCGTACTCGG 
GTGATGGAGT ACATTAACAG ACTAGACAAC TACGACGCTC CGGACATTGC TAATATCGCC 
ATCAGCAATG AGCTATTTGA AGAAGCGTTT GCCATTTTCA AGAAGTTTGA TGTCAACACC 
TCTGCAATAC AGGTATTGAT AGAGCACATA GGGAACCTGG ACCGAGCCTA CGAGTTTGCA 
GAGCGCTGTA ACGAACCTGC GGTGTGGAGC CAGTTGGGCA GAGCTCAGCT GCACCGAGGC 
CTCGTCAAGG AGGCCATCGA CTCGTACATC AAAGCTGTCG ACCCTTCTGC GTACATGGAG 
GTGGTCAATG CAGCCAGCAA GAACAATAAT TGGGAAGATC TGGTGAAATT CCTCCAGATG 
GCTCGAAAGA AAGCCAGAGA GTCCTACGTG GAGACGGAGC TCATCTTCGC CTTGGCTAAA 
ACAAACCGTC TGGCCGAACT GGAGGAGTTC GTCAGTGGAC CCAACAATGC TCACATCCAG 
CAGGTGGGCG ATAGATGCTA CGAGGAAGGC ATGTACGAGG CAGCGAAGCT CTTGTATAAC 
AACGTTTCCA ACTTTGCTCG TCTCGCCTCG ACACTCGTAC ATCTCGGGGA GTACCAGGCG 
GCCGTGGACA GTGCCAGGAA AGCCAACAGC ACACGGACAT GGAAGGAGGT CTGCTTTGCG 
TGCGTGGATG GGGAGGAGTT CCGTCTGGCG CAAATCTGCG GCCTCCACAT CGTCATCCAC 
GCTGACGAGC TGGAAGACCT GATCAGCTAC TATCAGGACC GCGGATACTT CGAGGAACTC 
ATTGCGCTGC TGGAGGCGGC GCTGGGTTTG GAGCGGGCTC ACATGGGCAT GTTCACAGAG 
CTCGCCATCC TCTACTCCAA GTTCAAACCT CAGAAGATGA GGGAACACTT GGAGCTCTTC 
TGGTCCAGAG TCAATATTCC AAAGGTCCTC CGTGCAGCAG AGCAGTCTCA CCTCTGGGCA 
GAGCTGGTTT TCCTTTACGA TAAATACGAG GAGTACGACA ACGCAGTCCT CACGATGATG 
TCTCATCCGA CTGAAGCTTG GAAAGAAGGG CTGTTCAAAG ACATTATCCC AAAAGTGGCC 
AATGTGGAAC TGTACTATAA ATCCCTCTCC TTCTATCTGG ACTATAAACC GCTCCTACTC 
AACGACCTGC TGACCATCTT GTCTCCACGG CTTGATCACA GCCGAGCGGT CTCGTTTTTC 
TCAAAGATGA ACCAGCTGAA GCTGGTTAAG CCTTACCTGA GATCAGTCCA GAACCACAAC 
AACAAGTCTG TCAACGAGGC ACTCAACAAC CTGTTGACAG AGGAGGAAGA CTATCAGGGT 
CTGAGAGCGT CCATCGATGC CTACGACAAC TTTGATACCA TTGGCCTGGC GCAGAGATTA 
GAGAAGCACG ACCTGATCGA GTTCAGACGC ATTGCTGCTT ATCTTTATAA GGGTAACAAC 
CGCTGGAGAC AGAGTGTTGA GCTCTGCAAG AAGGACAAAC TCTACAAGGA TGCCATGCTG 
TACGCTGCAG AGTCGAAGGA TGCTGAGCTG GCAGAAAATC TGCTTCAGTG GTTCCTGGAG 
GAAGGCAGGA AAGAGTGCTT TGCTGCTTGC CTCTTTGCAT CCTATGACCT GCTGCATCCC 
GACGTAGTGC TGGAGCTGGC CTGGAGGCAC AATATCATGG ACTTCGCCAT GCCGTACTTC 
ATCCAGGTCA TGAGGGAGTA CCTCACAAAG GTGGACAAAC TGGAGGAGGC AGAGAGCCAG 
AGAAAAACGG AGGAGGAGGT GACAGAACCT CAGCCGATGG TGTTTGGCCA GCAGCTGATG 
TTGACCGCCT CACCTGCTCC AGTGACCCCC CAGACGGGCT ACCCGAGCTA TGCCTACCCC 
CCCGCAGGTT ACCCTGCCGC CCCCGCTGCT GGCTACCCCC CTCAACCCCC AGCATACGGC 
TTCAACATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029685576.1
CDS	401..5452
Translation	MAQILPIRFQEHLQLQNMGVNPANIGFSYLTMESDKFICIREKVGDQNQVVIVDMSDPTNPIRRPISADSAIMNPASKVIALKDAAKTLQIFNIEMKSKLKAHTMTEDVMFWKWISVNTVALVTDSAVYHWSMEGDSQPAKVFDRHASLAGCQIINYRTDQQQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFGDFKVEGNTKPSTLFCFAVRSQAGGKLHIIEVGQPAAGNQPFAKKAVDVFFPPEAQTDFPVAMQIGSKHGVIYLITKYGYIHLYDLESGVCIYMNRISAETIFVTAPHEATSGIIGVNKKGQVLSVCVEEENIVNYATNVLQNPDLALRIALRSNLAGAEEIFARKFNTLFSQGNYSEAAKVAASAPKGILRTAETIRKFQSVPAQPGQASPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKASDPTLALSVYLRANVPNKVIQCFAETGQFQKIVLYAKKVGYTPDWVFLLRNVMRVNPDQGLQFAQMLVQDEEPLANINQIVDVFMEGNLIQQCTSFLLDALKNNRPAEGHLQTRLLEMNLIHAPQVADAILGNQMFTHYDRAHVAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVEDSLECLRAMLSANIRQNLQLCVQVASKYHEQLGTQSLVELFESFKSYEGLFYFLGSIVNFSQEPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCAEDVIKNLIIVVRGQFSTDELVEEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNTPERFLKENPFYDSAVVGKYCEKRDPHLACVAYERGQCDLDLIKVCNENSLFKSEARYLVHRKDPELWANVLEENNPFRRQLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFKKFDVNTSAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLGRAQLHRGLVKEAIDSYIKAVDPSAYMEVVNAASKNNNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFVSGPNNAHIQQVGDRCYEEGMYEAAKLLYNNVSNFARLASTLVHLGEYQAAVDSARKANSTRTWKEVCFACVDGEEFRLAQICGLHIVIHADELEDLISYYQDRGYFEELIALLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQSHLWAELVFLYDKYEEYDNAVLTMMSHPTEAWKEGLFKDIIPKVANVELYYKSLSFYLDYKPLLLNDLLTILSPRLDHSRAVSFFSKMNQLKLVKPYLRSVQNHNNKSVNEALNNLLTEEEDYQGLRASIDAYDNFDTIGLAQRLEKHDLIEFRRIAAYLYKGNNRWRQSVELCKKDKLYKDAMLYAAESKDAELAENLLQWFLEEGRKECFAACLFASYDLLHPDVVLELAWRHNIMDFAMPYFIQVMREYLTKVDKLEEAESQRKTEEEVTEPQPMVFGQQLMLTASPAPVTPQTGYPSYAYPPAGYPAAPAAGYPPQPPAYGFNM

Target ORF information:

RefSeq Version	XM_029829716.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029829716.1

ORF Insert Sequence:

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG ACGCTGCCAA GACCCTGCAG ATCTTTAACA TTGAGATGAA AAGTAAACTA 
AAGGCTCACA CCATGACAGA AGATGTGATG TTCTGGAAGT GGATATCGGT GAACACCGTC 
GCCCTGGTGA CTGATTCAGC CGTCTATCAC TGGAGCATGG AGGGAGACTC TCAGCCCGCT 
AAGGTTTTCG ACCGGCACGC CAGTCTGGCA GGCTGTCAGA TCATCAACTA CAGAACTGAT 
CAGCAACAGA AGTGGCTGCT TTTGATCGGG ATCTCCGCGC AGCAAAACCG CGTGGTTGGA 
GCCATGCAGC TGTATTCAGT CGACAGGAAA GTGTCCCAGC CCATCGAGGG ACACGCTGCT 
GCCTTTGGAG ACTTCAAAGT GGAGGGGAAC ACCAAACCTT CCACCCTCTT TTGCTTCGCC 
GTGCGCTCGC AAGCTGGGGG AAAGTTGCAC ATCATTGAAG TCGGTCAGCC AGCTGCAGGG 
AACCAGCCCT TTGCTAAGAA AGCCGTTGAT GTGTTTTTCC CTCCAGAGGC GCAGACAGAC 
TTTCCCGTAG CCATGCAGAT CGGAAGTAAA CATGGAGTCA TATATTTGAT CACAAAGTAT 
GGTTACATTC ACTTGTACGA CCTGGAGTCT GGAGTTTGCA TCTACATGAA TCGAATTAGC 
GCGGAGACCA TTTTTGTCAC GGCTCCACAT GAAGCCACCT CAGGAATCAT TGGGGTGAAC 
AAGAAGGGTC AGGTCCTCTC AGTCTGCGTT GAAGAGGAAA ACATCGTCAA CTACGCCACC 
AACGTTTTAC AGAACCCAGA CCTGGCGTTG AGGATAGCTT TAAGGTCCAA TCTCGCTGGG 
GCGGAGGAGA TTTTTGCGAG GAAGTTCAAC ACACTGTTCT CCCAGGGAAA TTATTCAGAG 
GCTGCAAAGG TTGCTGCCTC GGCACCTAAG GGAATTCTCC GCACTGCAGA GACCATTCGT 
AAGTTCCAGA GCGTCCCAGC TCAGCCAGGT CAGGCCTCCC CATTGTTGCA GTACTTTGGT 
ATTCTGCTGG ACCAGGGTCA GCTCAACAAG TTTGAGTCTT TGGAGCTGTG CCGGCCTGTC 
CTGCAGCAGG GCCGCAAGCA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GTTGGAGTGC 
TCAGAAGAGC TGGGAGACCT GGTGAAGGCA TCGGATCCCA CTTTAGCTCT TAGCGTGTAC 
CTCAGAGCTA ACGTCCCCAA CAAGGTCATC CAGTGTTTTG CTGAAACTGG CCAGTTCCAG 
AAGATTGTGC TTTATGCCAA AAAGGTGGGC TACACCCCTG ACTGGGTTTT CCTCCTGAGA 
AACGTGATGC GTGTAAATCC AGACCAGGGC CTGCAGTTCG CCCAGATGCT TGTCCAGGAC 
GAGGAGCCGC TGGCCAACAT AAACCAGATT GTTGACGTCT TCATGGAGGG AAATCTGATC 
CAGCAGTGCA CGTCCTTCCT GTTGGACGCT CTGAAGAACA ACCGTCCCGC CGAAGGACAC 
TTACAGACTC GCCTGCTTGA GATGAACCTC ATACATGCCC CCCAGGTCGC GGATGCCATC 
CTTGGGAACC AGATGTTCAC CCATTATGAC CGGGCCCATG TGGCCCAGCT GTGCGAGAAG 
GCAGGTCTCC TGCAAAGAGC TCTTGAACAC TACACAGATC TGTACGACAT CAAACGGGCA 
GTGGTGCACA CGCATCTGCT CAACCCTGAG TGGCTGGTGA ACTTTTTTGG CTCGTTGTCA 
GTTGAAGACT CTTTGGAATG TCTGAGAGCC ATGTTGTCAG CTAACATCAG GCAGAACCTG 
CAGCTCTGCG TCCAGGTAGC ATCGAAGTAT CACGAGCAGC TGGGGACTCA GTCTTTAGTG 
GAACTCTTTG AATCCTTCAA GAGTTATGAG GGTTTGTTTT ACTTCCTTGG ATCGATTGTG 
AATTTCAGTC AGGAGCCTGA TGTTCACTTT AAGTACATCC AGGCTGCCTG CAAAACAGGT 
CAGATCAAGG AAGTGGAGAG AATCTGTCGA GAGAGCAACT GCTACGACCC AGAGAGAGTC 
AAAAACTTCC TCAAGGAGGC CAAGCTTACA GATCAGCTCC CTCTGATCAT CGTGTGTGAT 
CGCTTTGACT TTGTTCACGA CTTGGTGCTC TATCTCTACC GCAACAATCT CCAGAAATAC 
ATTGAAATCT ACGTGCAAAA GGTGAATCCC AGTCGTTTAC CGGTGGTTAT AGGTGGACTG 
TTGGATGTCG ACTGTGCTGA GGATGTGATC AAGAACCTGA TCATTGTGGT CAGAGGGCAG 
TTTTCCACTG ATGAGCTAGT AGAGGAAGTA GAAAAGAGGA ACAGGTTAAA GCTGTTGTTA 
CCATGGCTTG AGTCTCGCAT CCATGAAGGG TGCGAGGAAC CGGCCACTCA CAATGCGCTG 
GCCAAGATCT ATATTGATAG CAACAATACG CCCGAGCGTT TCCTGAAAGA GAACCCATTC 
TACGACAGCG CTGTTGTTGG AAAATACTGT GAGAAGAGAG ACCCTCACCT GGCCTGTGTA 
GCTTATGAGA GAGGACAATG TGACCTGGAC CTTATAAAGG TGTGCAATGA AAACTCCTTA 
TTCAAGAGTG AGGCTAGATA TCTGGTCCAT CGGAAAGACC CAGAGCTTTG GGCAAATGTG 
CTTGAAGAAA ACAACCCATT CAGACGACAA CTTATCGATC AGGTGGTGCA GACAGCCCTG 
TCAGAGACCC AGGACCCAGA GGAGGTTTCA GTAACCGTCA AGGCCTTCAT GACTGCAGAC 
CTTCCCAATG AGCTGATTGA ACTGCTGGAA AAGATCGTAC TCGACAACTC TGTCTTTAGC 
GAGCACAGAA ACCTCCAGAA CCTGCTCATT CTGACTGCCA TCAAGGCCGA CCGTACTCGG 
GTGATGGAGT ACATTAACAG ACTAGACAAC TACGACGCTC CGGACATTGC TAATATCGCC 
ATCAGCAATG AGCTATTTGA AGAAGCGTTT GCCATTTTCA AGAAGTTTGA TGTCAACACC 
TCTGCAATAC AGGTATTGAT AGAGCACATA GGGAACCTGG ACCGAGCCTA CGAGTTTGCA 
GAGCGCTGTA ACGAACCTGC GGTGTGGAGC CAGTTGGGCA GAGCTCAGCT GCACCGAGGC 
CTCGTCAAGG AGGCCATCGA CTCGTACATC AAAGCTGTCG ACCCTTCTGC GTACATGGAG 
GTGGTCAATG CAGCCAGCAA GAACAATAAT TGGGAAGATC TGGTGAAATT CCTCCAGATG 
GCTCGAAAGA AAGCCAGAGA GTCCTACGTG GAGACGGAGC TCATCTTCGC CTTGGCTAAA 
ACAAACCGTC TGGCCGAACT GGAGGAGTTC GTCAGTGGAC CCAACAATGC TCACATCCAG 
CAGGTGGGCG ATAGATGCTA CGAGGAAGGC ATGTACGAGG CAGCGAAGCT CTTGTATAAC 
AACGTTTCCA ACTTTGCTCG TCTCGCCTCG ACACTCGTAC ATCTCGGGGA GTACCAGGCG 
GCCGTGGACA GTGCCAGGAA AGCCAACAGC ACACGGACAT GGAAGGAGGT CTGCTTTGCG 
TGCGTGGATG GGGAGGAGTT CCGTCTGGCG CAAATCTGCG GCCTCCACAT CGTCATCCAC 
GCTGACGAGC TGGAAGACCT GATCAGCTAC TATCAGGACC GCGGATACTT CGAGGAACTC 
ATTGCGCTGC TGGAGGCGGC GCTGGGTTTG GAGCGGGCTC ACATGGGCAT GTTCACAGAG 
CTCGCCATCC TCTACTCCAA GTTCAAACCT CAGAAGATGA GGGAACACTT GGAGCTCTTC 
TGGTCCAGAG TCAATATTCC AAAGGTCCTC CGTGCAGCAG AGCAGTCTCA CCTCTGGGCA 
GAGCTGGTTT TCCTTTACGA TAAATACGAG GAGTACGACA ACGCAGTCCT CACGATGATG 
TCTCATCCGA CTGAAGCTTG GAAAGAAGGG CTGTTCAAAG ACATTATCCC AAAAGTGGCC 
AATGTGGAAC TGTACTATAA ATCCCTCTCC TTCTATCTGG ACTATAAACC GCTCCTACTC 
AACGACCTGC TGACCATCTT GTCTCCACGG CTTGATCACA GCCGAGCGGT CTCGTTTTTC 
TCAAAGATGA ACCAGCTGAA GCTGGTTAAG CCTTACCTGA GATCAGTCCA GAACCACAAC 
AACAAGTCTG TCAACGAGGC ACTCAACAAC CTGTTGACAG AGGAGGAAGA CTATCAGGGT 
CTGAGAGCGT CCATCGATGC CTACGACAAC TTTGATACCA TTGGCCTGGC GCAGAGATTA 
GAGAAGCACG ACCTGATCGA GTTCAGACGC ATTGCTGCTT ATCTTTATAA GGGTAACAAC 
CGCTGGAGAC AGAGTGTTGA GCTCTGCAAG AAGGACAAAC TCTACAAGGA TGCCATGCTG 
TACGCTGCAG AGTCGAAGGA TGCTGAGCTG GCAGAAAATC TGCTTCAGTG GTTCCTGGAG 
GAAGGCAGGA AAGAGTGCTT TGCTGCTTGC CTCTTTGCAT CCTATGACCT GCTGCATCCC 
GACGTAGTGC TGGAGCTGGC CTGGAGGCAC AATATCATGG ACTTCGCCAT GCCGTACTTC 
ATCCAGGTCA TGAGGGAGTA CCTCACAAAG GTGGACAAAC TGGAGGAGGC AGAGAGCCAG 
AGAAAAACGG AGGAGGAGGT GACAGAACCT CAGCCGATGG TGTTTGGCCA GCAGCTGATG 
TTGACCGCCT CACCTGCTCC AGTGACCCCC CAGACGGGCT ACCCGAGCTA TGCCTACCCC 
CCCGCAGGTT ACCCTGCCGC CCCCGCTGCT GGCTACCCCC CTCAACCCCC AGCATACGGC 
TTCAACATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101077362 ( XM_003975152.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003975201.1 Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X4, mRNA.

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CloneID	OTo60676
Clone ID Related Accession (Same CDS sequence)	XM_003975152.3
Accession Version	XM_003975152.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	clathrin heavy chain 1-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042305.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003975152.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG CTGCCAAGAC CCTGCAGATC TTTAACATTG AGATGAAAAG TAAACTAAAG 
GCTCACACCA TGACAGAAGA TGTGATGTTC TGGAAGTGGA TATCGGTGAA CACCGTCGCC 
CTGGTGACTG ATTCAGCCGT CTATCACTGG AGCATGGAGG GAGACTCTCA GCCCGCTAAG 
GTTTTCGACC GGCACGCCAG TCTGGCAGGC TGTCAGATCA TCAACTACAG AACTGATCAG 
CAACAGAAGT GGCTGCTTTT GATCGGGATC TCCGCGCAGC AAAACCGCGT GGTTGGAGCC 
ATGCAGCTGT ATTCAGTCGA CAGGAAAGTG TCCCAGCCCA TCGAGGGACA CGCTGCTGCC 
TTTGGAGACT TCAAAGTGGA GGGGAACACC AAACCTTCCA CCCTCTTTTG CTTCGCCGTG 
CGCTCGCAAG CTGGGGGAAA GTTGCACATC ATTGAAGTCG GTCAGCCAGC TGCAGGGAAC 
CAGCCCTTTG CTAAGAAAGC CGTTGATGTG TTTTTCCCTC CAGAGGCGCA GACAGACTTT 
CCCGTAGCCA TGCAGATCGG AAGTAAACAT GGAGTCATAT ATTTGATCAC AAAGTATGGT 
TACATTCACT TGTACGACCT GGAGTCTGGA GTTTGCATCT ACATGAATCG AATTAGCGCG 
GAGACCATTT TTGTCACGGC TCCACATGAA GCCACCTCAG GAATCATTGG GGTGAACAAG 
AAGGGTCAGG TCCTCTCAGT CTGCGTTGAA GAGGAAAACA TCGTCAACTA CGCCACCAAC 
GTTTTACAGA ACCCAGACCT GGCGTTGAGG ATAGCTTTAA GGTCCAATCT CGCTGGGGCG 
GAGGAGATTT TTGCGAGGAA GTTCAACACA CTGTTCTCCC AGGGAAATTA TTCAGAGGCT 
GCAAAGGTTG CTGCCTCGGC ACCTAAGGGA ATTCTCCGCA CTGCAGAGAC CATTCGTAAG 
TTCCAGAGCG TCCCAGCTCA GCCAGGTCAG GCCTCCCCAT TGTTGCAGTA CTTTGGTATT 
CTGCTGGACC AGGGTCAGCT CAACAAGTTT GAGTCTTTGG AGCTGTGCCG GCCTGTCCTG 
CAGCAGGGCC GCAAGCAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGTT GGAGTGCTCA 
GAAGAGCTGG GAGACCTGGT GAAGGCATCG GATCCCACTT TAGCTCTTAG CGTGTACCTC 
AGAGCTAACG TCCCCAACAA GGTCATCCAG TGTTTTGCTG AAACTGGCCA GTTCCAGAAG 
ATTGTGCTTT ATGCCAAAAA GGTGGGCTAC ACCCCTGACT GGGTTTTCCT CCTGAGAAAC 
GTGATGCGTG TAAATCCAGA CCAGGGCCTG CAGTTCGCCC AGATGCTTGT CCAGGACGAG 
GAGCCGCTGG CCAACATAAA CCAGATTGTT GACGTCTTCA TGGAGGGAAA TCTGATCCAG 
CAGTGCACGT CCTTCCTGTT GGACGCTCTG AAGAACAACC GTCCCGCCGA AGGACACTTA 
CAGACTCGCC TGCTTGAGAT GAACCTCATA CATGCCCCCC AGGTCGCGGA TGCCATCCTT 
GGGAACCAGA TGTTCACCCA TTATGACCGG GCCCATGTGG CCCAGCTGTG CGAGAAGGCA 
GGTCTCCTGC AAAGAGCTCT TGAACACTAC ACAGATCTGT ACGACATCAA ACGGGCAGTG 
GTGCACACGC ATCTGCTCAA CCCTGAGTGG CTGGTGAACT TTTTTGGCTC GTTGTCAGTT 
GAAGACTCTT TGGAATGTCT GAGAGCCATG TTGTCAGCTA ACATCAGGCA GAACCTGCAG 
CTCTGCGTCC AGGTAGCATC GAAGTATCAC GAGCAGCTGG GGACTCAGTC TTTAGTGGAA 
CTCTTTGAAT CCTTCAAGAG TTATGAGGGT TTGTTTTACT TCCTTGGATC GATTGTGAAT 
TTCAGTCAGG AGCCTGATGT TCACTTTAAG TACATCCAGG CTGCCTGCAA AACAGGTCAG 
ATCAAGGAAG TGGAGAGAAT CTGTCGAGAG AGCAACTGCT ACGACCCAGA GAGAGTCAAA 
AACTTCCTCA AGGAGGCCAA GCTTACAGAT CAGCTCCCTC TGATCATCGT GTGTGATCGC 
TTTGACTTTG TTCACGACTT GGTGCTCTAT CTCTACCGCA ACAATCTCCA GAAATACATT 
GAAATCTACG TGCAAAAGGT GAATCCCAGT CGTTTACCGG TGGTTATAGG TGGACTGTTG 
GATGTCGACT GTGCTGAGGA TGTGATCAAG AACCTGATCA TTGTGGTCAG AGGGCAGTTT 
TCCACTGATG AGCTAGTAGA GGAAGTAGAA AAGAGGAACA GGTTAAAGCT GTTGTTACCA 
TGGCTTGAGT CTCGCATCCA TGAAGGGTGC GAGGAACCGG CCACTCACAA TGCGCTGGCC 
AAGATCTATA TTGATAGCAA CAATACGCCC GAGCGTTTCC TGAAAGAGAA CCCATTCTAC 
GACAGCGCTG TTGTTGGAAA ATACTGTGAG AAGAGAGACC CTCACCTGGC CTGTGTAGCT 
TATGAGAGAG GACAATGTGA CCTGGACCTT ATAAAGGTGT GCAATGAAAA CTCCTTATTC 
AAGAGTGAGG CTAGATATCT GGTCCATCGG AAAGACCCAG AGCTTTGGGC AAATGTGCTT 
GAAGAAAACA ACCCATTCAG ACGACAACTT ATCGATCAGG TGGTGCAGAC AGCCCTGTCA 
GAGACCCAGG ACCCAGAGGA GGTTTCAGTA ACCGTCAAGG CCTTCATGAC TGCAGACCTT 
CCCAATGAGC TGATTGAACT GCTGGAAAAG ATCGTACTCG ACAACTCTGT CTTTAGCGAG 
CACAGAAACC TCCAGAACCT GCTCATTCTG ACTGCCATCA AGGCCGACCG TACTCGGGTG 
ATGGAGTACA TTAACAGACT AGACAACTAC GACGCTCCGG ACATTGCTAA TATCGCCATC 
AGCAATGAGC TATTTGAAGA AGCGTTTGCC ATTTTCAAGA AGTTTGATGT CAACACCTCT 
GCAATACAGG TATTGATAGA GCACATAGGG AACCTGGACC GAGCCTACGA GTTTGCAGAG 
CGCTGTAACG AACCTGCGGT GTGGAGCCAG TTGGGCAGAG CTCAGCTGCA CCGAGGCCTC 
GTCAAGGAGG CCATCGACTC GTACATCAAA GCTGTCGACC CTTCTGCGTA CATGGAGGTG 
GTCAATGCAG CCAGCAAGAA CAATAATTGG GAAGATCTGG TGAAATTCCT CCAGATGGCT 
CGAAAGAAAG CCAGAGAGTC CTACGTGGAG ACGGAGCTCA TCTTCGCCTT GGCTAAAACA 
AACCGTCTGG CCGAACTGGA GGAGTTCGTC AGTGGACCCA ACAATGCTCA CATCCAGCAG 
GTGGGCGATA GATGCTACGA GGAAGGCATG TACGAGGCAG CGAAGCTCTT GTATAACAAC 
GTTTCCAACT TTGCTCGTCT CGCCTCGACA CTCGTACATC TCGGGGAGTA CCAGGCGGCC 
GTGGACAGTG CCAGGAAAGC CAACAGCACA CGGACATGGA AGGAGGTCTG CTTTGCGTGC 
GTGGATGGGG AGGAGTTCCG TCTGGCGCAA ATCTGCGGCC TCCACATCGT CATCCACGCT 
GACGAGCTGG AAGACCTGAT CAGCTACTAT CAGGACCGCG GATACTTCGA GGAACTCATT 
GCGCTGCTGG AGGCGGCGCT GGGTTTGGAG CGGGCTCACA TGGGCATGTT CACAGAGCTC 
GCCATCCTCT ACTCCAAGTT CAAACCTCAG AAGATGAGGG AACACTTGGA GCTCTTCTGG 
TCCAGAGTCA ATATTCCAAA GGTCCTCCGT GCAGCAGAGC AGTCTCACCT CTGGGCAGAG 
CTGGTTTTCC TTTACGATAA ATACGAGGAG TACGACAACG CAGTCCTCAC GATGATGTCT 
CATCCGACTG AAGCTTGGAA AGAAGGGCTG TTCAAAGACA TTATCCCAAA AGTGGCCAAT 
GTGGAACTGT ACTATAAATC CCTCTCCTTC TATCTGGACT ATAAACCGCT CCTACTCAAC 
GACCTGCTGA CCATCTTGTC TCCACGGCTT GATCACAGCC GAGCGGTCTC GTTTTTCTCA 
AAGATGAACC AGCTGAAGCT GGTTAAGCCT TACCTGAGAT CAGTCCAGAA CCACAACAAC 
AAGTCTGTCA ACGAGGCACT CAACAACCTG TTGACAGAGG AGGAAGACTA TCAGGGTCTG 
AGAGCGTCCA TCGATGCCTA CGACAACTTT GATACCATTG GCCTGGCGCA GAGATTAGAG 
AAGCACGACC TGATCGAGTT CAGACGCATT GCTGCTTATC TTTATAAGGG TAACAACCGC 
TGGAGACAGA GTGTTGAGCT CTGCAAGAAG GACAAACTCT ACAAGGATGC CATGCTGTAC 
GCTGCAGAGT CGAAGGATGC TGAGCTGGCA GAAAATCTGC TTCAGTGGTT CCTGGAGGAA 
GGCAGGAAAG AGTGCTTTGC TGCTTGCCTC TTTGCATCCT ATGACCTGCT GCATCCCGAC 
GTAGTGCTGG AGCTGGCCTG GAGGCACAAT ATCATGGACT TCGCCATGCC GTACTTCATC 
CAGGTCATGA GGGAGTACCT CACAAAGGTG GACAAACTGG AGGAGGCAGA GAGCCAGAGA 
AAAACGGAGG AGGAGGTGAC AGAACCTCAG CCGATGGTGT TTGGCCAGCA GCTGATGTTG 
ACCGCCTCAC CTGCTCCAGT GACCCCCCAG ACGGGCTACC CGAGCTATGC CTACCCCCCC 
GCAGGTTACC CTGCCGCCCC CGCTGCTGGC TACCCCCCTC AACCCCCAGC ATACGGCTTC 
AACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003975201.1
CDS	401..5449
Translation	MAQILPIRFQEHLQLQNMGVNPANIGFSYLTMESDKFICIREKVGDQNQVVIVDMSDPTNPIRRPISADSAIMNPASKVIALKAAKTLQIFNIEMKSKLKAHTMTEDVMFWKWISVNTVALVTDSAVYHWSMEGDSQPAKVFDRHASLAGCQIINYRTDQQQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFGDFKVEGNTKPSTLFCFAVRSQAGGKLHIIEVGQPAAGNQPFAKKAVDVFFPPEAQTDFPVAMQIGSKHGVIYLITKYGYIHLYDLESGVCIYMNRISAETIFVTAPHEATSGIIGVNKKGQVLSVCVEEENIVNYATNVLQNPDLALRIALRSNLAGAEEIFARKFNTLFSQGNYSEAAKVAASAPKGILRTAETIRKFQSVPAQPGQASPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKASDPTLALSVYLRANVPNKVIQCFAETGQFQKIVLYAKKVGYTPDWVFLLRNVMRVNPDQGLQFAQMLVQDEEPLANINQIVDVFMEGNLIQQCTSFLLDALKNNRPAEGHLQTRLLEMNLIHAPQVADAILGNQMFTHYDRAHVAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVEDSLECLRAMLSANIRQNLQLCVQVASKYHEQLGTQSLVELFESFKSYEGLFYFLGSIVNFSQEPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCAEDVIKNLIIVVRGQFSTDELVEEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNTPERFLKENPFYDSAVVGKYCEKRDPHLACVAYERGQCDLDLIKVCNENSLFKSEARYLVHRKDPELWANVLEENNPFRRQLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFKKFDVNTSAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLGRAQLHRGLVKEAIDSYIKAVDPSAYMEVVNAASKNNNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFVSGPNNAHIQQVGDRCYEEGMYEAAKLLYNNVSNFARLASTLVHLGEYQAAVDSARKANSTRTWKEVCFACVDGEEFRLAQICGLHIVIHADELEDLISYYQDRGYFEELIALLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQSHLWAELVFLYDKYEEYDNAVLTMMSHPTEAWKEGLFKDIIPKVANVELYYKSLSFYLDYKPLLLNDLLTILSPRLDHSRAVSFFSKMNQLKLVKPYLRSVQNHNNKSVNEALNNLLTEEEDYQGLRASIDAYDNFDTIGLAQRLEKHDLIEFRRIAAYLYKGNNRWRQSVELCKKDKLYKDAMLYAAESKDAELAENLLQWFLEEGRKECFAACLFASYDLLHPDVVLELAWRHNIMDFAMPYFIQVMREYLTKVDKLEEAESQRKTEEEVTEPQPMVFGQQLMLTASPAPVTPQTGYPSYAYPPAGYPAAPAAGYPPQPPAYGFNM

Target ORF information:

RefSeq Version	XM_003975152.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003975152.3

ORF Insert Sequence:

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG CTGCCAAGAC CCTGCAGATC TTTAACATTG AGATGAAAAG TAAACTAAAG 
GCTCACACCA TGACAGAAGA TGTGATGTTC TGGAAGTGGA TATCGGTGAA CACCGTCGCC 
CTGGTGACTG ATTCAGCCGT CTATCACTGG AGCATGGAGG GAGACTCTCA GCCCGCTAAG 
GTTTTCGACC GGCACGCCAG TCTGGCAGGC TGTCAGATCA TCAACTACAG AACTGATCAG 
CAACAGAAGT GGCTGCTTTT GATCGGGATC TCCGCGCAGC AAAACCGCGT GGTTGGAGCC 
ATGCAGCTGT ATTCAGTCGA CAGGAAAGTG TCCCAGCCCA TCGAGGGACA CGCTGCTGCC 
TTTGGAGACT TCAAAGTGGA GGGGAACACC AAACCTTCCA CCCTCTTTTG CTTCGCCGTG 
CGCTCGCAAG CTGGGGGAAA GTTGCACATC ATTGAAGTCG GTCAGCCAGC TGCAGGGAAC 
CAGCCCTTTG CTAAGAAAGC CGTTGATGTG TTTTTCCCTC CAGAGGCGCA GACAGACTTT 
CCCGTAGCCA TGCAGATCGG AAGTAAACAT GGAGTCATAT ATTTGATCAC AAAGTATGGT 
TACATTCACT TGTACGACCT GGAGTCTGGA GTTTGCATCT ACATGAATCG AATTAGCGCG 
GAGACCATTT TTGTCACGGC TCCACATGAA GCCACCTCAG GAATCATTGG GGTGAACAAG 
AAGGGTCAGG TCCTCTCAGT CTGCGTTGAA GAGGAAAACA TCGTCAACTA CGCCACCAAC 
GTTTTACAGA ACCCAGACCT GGCGTTGAGG ATAGCTTTAA GGTCCAATCT CGCTGGGGCG 
GAGGAGATTT TTGCGAGGAA GTTCAACACA CTGTTCTCCC AGGGAAATTA TTCAGAGGCT 
GCAAAGGTTG CTGCCTCGGC ACCTAAGGGA ATTCTCCGCA CTGCAGAGAC CATTCGTAAG 
TTCCAGAGCG TCCCAGCTCA GCCAGGTCAG GCCTCCCCAT TGTTGCAGTA CTTTGGTATT 
CTGCTGGACC AGGGTCAGCT CAACAAGTTT GAGTCTTTGG AGCTGTGCCG GCCTGTCCTG 
CAGCAGGGCC GCAAGCAACT GCTGGAGAAA TGGCTGAAGG AGGACAAGTT GGAGTGCTCA 
GAAGAGCTGG GAGACCTGGT GAAGGCATCG GATCCCACTT TAGCTCTTAG CGTGTACCTC 
AGAGCTAACG TCCCCAACAA GGTCATCCAG TGTTTTGCTG AAACTGGCCA GTTCCAGAAG 
ATTGTGCTTT ATGCCAAAAA GGTGGGCTAC ACCCCTGACT GGGTTTTCCT CCTGAGAAAC 
GTGATGCGTG TAAATCCAGA CCAGGGCCTG CAGTTCGCCC AGATGCTTGT CCAGGACGAG 
GAGCCGCTGG CCAACATAAA CCAGATTGTT GACGTCTTCA TGGAGGGAAA TCTGATCCAG 
CAGTGCACGT CCTTCCTGTT GGACGCTCTG AAGAACAACC GTCCCGCCGA AGGACACTTA 
CAGACTCGCC TGCTTGAGAT GAACCTCATA CATGCCCCCC AGGTCGCGGA TGCCATCCTT 
GGGAACCAGA TGTTCACCCA TTATGACCGG GCCCATGTGG CCCAGCTGTG CGAGAAGGCA 
GGTCTCCTGC AAAGAGCTCT TGAACACTAC ACAGATCTGT ACGACATCAA ACGGGCAGTG 
GTGCACACGC ATCTGCTCAA CCCTGAGTGG CTGGTGAACT TTTTTGGCTC GTTGTCAGTT 
GAAGACTCTT TGGAATGTCT GAGAGCCATG TTGTCAGCTA ACATCAGGCA GAACCTGCAG 
CTCTGCGTCC AGGTAGCATC GAAGTATCAC GAGCAGCTGG GGACTCAGTC TTTAGTGGAA 
CTCTTTGAAT CCTTCAAGAG TTATGAGGGT TTGTTTTACT TCCTTGGATC GATTGTGAAT 
TTCAGTCAGG AGCCTGATGT TCACTTTAAG TACATCCAGG CTGCCTGCAA AACAGGTCAG 
ATCAAGGAAG TGGAGAGAAT CTGTCGAGAG AGCAACTGCT ACGACCCAGA GAGAGTCAAA 
AACTTCCTCA AGGAGGCCAA GCTTACAGAT CAGCTCCCTC TGATCATCGT GTGTGATCGC 
TTTGACTTTG TTCACGACTT GGTGCTCTAT CTCTACCGCA ACAATCTCCA GAAATACATT 
GAAATCTACG TGCAAAAGGT GAATCCCAGT CGTTTACCGG TGGTTATAGG TGGACTGTTG 
GATGTCGACT GTGCTGAGGA TGTGATCAAG AACCTGATCA TTGTGGTCAG AGGGCAGTTT 
TCCACTGATG AGCTAGTAGA GGAAGTAGAA AAGAGGAACA GGTTAAAGCT GTTGTTACCA 
TGGCTTGAGT CTCGCATCCA TGAAGGGTGC GAGGAACCGG CCACTCACAA TGCGCTGGCC 
AAGATCTATA TTGATAGCAA CAATACGCCC GAGCGTTTCC TGAAAGAGAA CCCATTCTAC 
GACAGCGCTG TTGTTGGAAA ATACTGTGAG AAGAGAGACC CTCACCTGGC CTGTGTAGCT 
TATGAGAGAG GACAATGTGA CCTGGACCTT ATAAAGGTGT GCAATGAAAA CTCCTTATTC 
AAGAGTGAGG CTAGATATCT GGTCCATCGG AAAGACCCAG AGCTTTGGGC AAATGTGCTT 
GAAGAAAACA ACCCATTCAG ACGACAACTT ATCGATCAGG TGGTGCAGAC AGCCCTGTCA 
GAGACCCAGG ACCCAGAGGA GGTTTCAGTA ACCGTCAAGG CCTTCATGAC TGCAGACCTT 
CCCAATGAGC TGATTGAACT GCTGGAAAAG ATCGTACTCG ACAACTCTGT CTTTAGCGAG 
CACAGAAACC TCCAGAACCT GCTCATTCTG ACTGCCATCA AGGCCGACCG TACTCGGGTG 
ATGGAGTACA TTAACAGACT AGACAACTAC GACGCTCCGG ACATTGCTAA TATCGCCATC 
AGCAATGAGC TATTTGAAGA AGCGTTTGCC ATTTTCAAGA AGTTTGATGT CAACACCTCT 
GCAATACAGG TATTGATAGA GCACATAGGG AACCTGGACC GAGCCTACGA GTTTGCAGAG 
CGCTGTAACG AACCTGCGGT GTGGAGCCAG TTGGGCAGAG CTCAGCTGCA CCGAGGCCTC 
GTCAAGGAGG CCATCGACTC GTACATCAAA GCTGTCGACC CTTCTGCGTA CATGGAGGTG 
GTCAATGCAG CCAGCAAGAA CAATAATTGG GAAGATCTGG TGAAATTCCT CCAGATGGCT 
CGAAAGAAAG CCAGAGAGTC CTACGTGGAG ACGGAGCTCA TCTTCGCCTT GGCTAAAACA 
AACCGTCTGG CCGAACTGGA GGAGTTCGTC AGTGGACCCA ACAATGCTCA CATCCAGCAG 
GTGGGCGATA GATGCTACGA GGAAGGCATG TACGAGGCAG CGAAGCTCTT GTATAACAAC 
GTTTCCAACT TTGCTCGTCT CGCCTCGACA CTCGTACATC TCGGGGAGTA CCAGGCGGCC 
GTGGACAGTG CCAGGAAAGC CAACAGCACA CGGACATGGA AGGAGGTCTG CTTTGCGTGC 
GTGGATGGGG AGGAGTTCCG TCTGGCGCAA ATCTGCGGCC TCCACATCGT CATCCACGCT 
GACGAGCTGG AAGACCTGAT CAGCTACTAT CAGGACCGCG GATACTTCGA GGAACTCATT 
GCGCTGCTGG AGGCGGCGCT GGGTTTGGAG CGGGCTCACA TGGGCATGTT CACAGAGCTC 
GCCATCCTCT ACTCCAAGTT CAAACCTCAG AAGATGAGGG AACACTTGGA GCTCTTCTGG 
TCCAGAGTCA ATATTCCAAA GGTCCTCCGT GCAGCAGAGC AGTCTCACCT CTGGGCAGAG 
CTGGTTTTCC TTTACGATAA ATACGAGGAG TACGACAACG CAGTCCTCAC GATGATGTCT 
CATCCGACTG AAGCTTGGAA AGAAGGGCTG TTCAAAGACA TTATCCCAAA AGTGGCCAAT 
GTGGAACTGT ACTATAAATC CCTCTCCTTC TATCTGGACT ATAAACCGCT CCTACTCAAC 
GACCTGCTGA CCATCTTGTC TCCACGGCTT GATCACAGCC GAGCGGTCTC GTTTTTCTCA 
AAGATGAACC AGCTGAAGCT GGTTAAGCCT TACCTGAGAT CAGTCCAGAA CCACAACAAC 
AAGTCTGTCA ACGAGGCACT CAACAACCTG TTGACAGAGG AGGAAGACTA TCAGGGTCTG 
AGAGCGTCCA TCGATGCCTA CGACAACTTT GATACCATTG GCCTGGCGCA GAGATTAGAG 
AAGCACGACC TGATCGAGTT CAGACGCATT GCTGCTTATC TTTATAAGGG TAACAACCGC 
TGGAGACAGA GTGTTGAGCT CTGCAAGAAG GACAAACTCT ACAAGGATGC CATGCTGTAC 
GCTGCAGAGT CGAAGGATGC TGAGCTGGCA GAAAATCTGC TTCAGTGGTT CCTGGAGGAA 
GGCAGGAAAG AGTGCTTTGC TGCTTGCCTC TTTGCATCCT ATGACCTGCT GCATCCCGAC 
GTAGTGCTGG AGCTGGCCTG GAGGCACAAT ATCATGGACT TCGCCATGCC GTACTTCATC 
CAGGTCATGA GGGAGTACCT CACAAAGGTG GACAAACTGG AGGAGGCAGA GAGCCAGAGA 
AAAACGGAGG AGGAGGTGAC AGAACCTCAG CCGATGGTGT TTGGCCAGCA GCTGATGTTG 
ACCGCCTCAC CTGCTCCAGT GACCCCCCAG ACGGGCTACC CGAGCTATGC CTACCCCCCC 
GCAGGTTACC CTGCCGCCCC CGCTGCTGGC TACCCCCCTC AACCCCCAGC ATACGGCTTC 
AACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101077362 ( XM_029829717.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029685577.1 Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X5, mRNA.

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CloneID	OTo60677
Clone ID Related Accession (Same CDS sequence)	XM_029829717.1
Accession Version	XM_029829717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	clathrin heavy chain 1-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042305.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG ACGCTGCCAA GACCCTGCAG ATCTTTAACA TTGAGATGAA AAGTAAACTA 
AAGGCTCACA CCATGACAGA AGATGTGATG TTCTGGAAGT GGATATCGGT GAACACCGTC 
GCCCTGGTGA CTGATTCAGC CGTCTATCAC TGGAGCATGG AGGGAGACTC TCAGCCCGCT 
AAGGTTTTCG ACCGGCACGC CAGTCTGGCA GGCTGTCAGA TCATCAACTA CAGAACTGAT 
CAGCAACAGA AGTGGCTGCT TTTGATCGGG ATCTCCGCGC AGCAAAACCG CGTGGTTGGA 
GCCATGCAGC TGTATTCAGT CGACAGGAAA GTGTCCCAGC CCATCGAGGG ACACGCTGCT 
GCCTTTGGAG ACTTCAAAGT GGAGGGGAAC ACCAAACCTT CCACCCTCTT TTGCTTCGCC 
GTGCGCTCGC AAGCTGGGGG AAAGTTGCAC ATCATTGAAG TCGGTCAGCC AGCTGCAGGG 
AACCAGCCCT TTGCTAAGAA AGCCGTTGAT GTGTTTTTCC CTCCAGAGGC GCAGACAGAC 
TTTCCCGTAG CCATGCAGAT CGGAAGTAAA CATGGAGTCA TATATTTGAT CACAAAGTAT 
GGTTACATTC ACTTGTACGA CCTGGAGTCT GGAGTTTGCA TCTACATGAA TCGAATTAGC 
GCGGAGACCA TTTTTGTCAC GGCTCCACAT GAAGCCACCT CAGGAATCAT TGGGGTGAAC 
AAGAAGGGTC AGGTCCTCTC AGTCTGCGTT GAAGAGGAAA ACATCGTCAA CTACGCCACC 
AACGTTTTAC AGAACCCAGA CCTGGCGTTG AGGATAGCTT TAAGGTCCAA TCTCGCTGGG 
GCGGAGGAGA TTTTTGCGAG GAAGTTCAAC ACACTGTTCT CCCAGGGAAA TTATTCAGAG 
GCTGCAAAGG TTGCTGCCTC GGCACCTAAG GGAATTCTCC GCACTGCAGA GACCATTCGT 
AAGTTCCAGA GCGTCCCAGC TCAGCCAGGT CAGGCCTCCC CATTGTTGCA GTACTTTGGT 
ATTCTGCTGG ACCAGGGTCA GCTCAACAAG TTTGAGTCTT TGGAGCTGTG CCGGCCTGTC 
CTGCAGCAGG GCCGCAAGCA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GTTGGAGTGC 
TCAGAAGAGC TGGGAGACCT GGTGAAGGCA TCGGATCCCA CTTTAGCTCT TAGCGTGTAC 
CTCAGAGCTA ACGTCCCCAA CAAGGTCATC CAGTGTTTTG CTGAAACTGG CCAGTTCCAG 
AAGATTGTGC TTTATGCCAA AAAGGTGGGC TACACCCCTG ACTGGGTTTT CCTCCTGAGA 
AACGTGATGC GTGTAAATCC AGACCAGGGC CTGCAGTTCG CCCAGATGCT TGTCCAGGAC 
GAGGAGCCGC TGGCCAACAT AAACCAGATT GTTGACGTCT TCATGGAGGG AAATCTGATC 
CAGCAGTGCA CGTCCTTCCT GTTGGACGCT CTGAAGAACA ACCGTCCCGC CGAAGGACAC 
TTACAGACTC GCCTGCTTGA GATGAACCTC ATACATGCCC CCCAGGTCGC GGATGCCATC 
CTTGGGAACC AGATGTTCAC CCATTATGAC CGGGCCCATG TGGCCCAGCT GTGCGAGAAG 
GCAGGTCTCC TGCAAAGAGC TCTTGAACAC TACACAGATC TGTACGACAT CAAACGGGCA 
GTGGTGCACA CGCATCTGCT CAACCCTGAG TGGCTGGTGA ACTTTTTTGG CTCGTTGTCA 
GTTGAAGACT CTTTGGAATG TCTGAGAGCC ATGTTGTCAG CTAACATCAG GCAGAACCTG 
CAGCTCTGCG TCCAGGTAGC ATCGAAGTAT CACGAGCAGC TGGGGACTCA GTCTTTAGTG 
GAACTCTTTG AATCCTTCAA GAGTTATGAG GGTTTGTTTT ACTTCCTTGG ATCGATTGTG 
AATTTCAGTC AGGAGCCTGA TGTTCACTTT AAGTACATCC AGGCTGCCTG CAAAACAGGT 
CAGATCAAGG AAGTGGAGAG AATCTGTCGA GAGAGCAACT GCTACGACCC AGAGAGAGTC 
AAAAACTTCC TCAAGGAGGC CAAGCTTACA GATCAGCTCC CTCTGATCAT CGTGTGTGAT 
CGCTTTGACT TTGTTCACGA CTTGGTGCTC TATCTCTACC GCAACAATCT CCAGAAATAC 
ATTGAAATCT ACGTGCAAAA GGTGAATCCC AGTCGTTTAC CGGTGGTTAT AGGTGGACTG 
TTGGATGTCG ACTGTGCTGA GGATGTGATC AAGAACCTGA TCATTGTGGT CAGAGGGCAG 
TTTTCCACTG ATGAGCTAGT AGAGGAAGTA GAAAAGAGGA ACAGGTTAAA GCTGTTGTTA 
CCATGGCTTG AGTCTCGCAT CCATGAAGGG TGCGAGGAAC CGGCCACTCA CAATGCGCTG 
GCCAAGATCT ATATTGATAG CAACAATACG CCCGAGCGTT TCCTGAAAGA GAACCCATTC 
TACGACAGCG CTGTTGTTGG AAAATACTGT GAGAAGAGAG ACCCTCACCT GGCCTGTGTA 
GCTTATGAGA GAGGACAATG TGACCTGGAC CTTATAAAGG TGTGCAATGA AAACTCCTTA 
TTCAAGAGTG AGGCTAGATA TCTGGTCCAT CGGAAAGACC CAGAGCTTTG GGCAAATGTG 
CTTGAAGAAA ACAACCCATT CAGACGACAA CTTATCGATC AGGTGGTGCA GACAGCCCTG 
TCAGAGACCC AGGACCCAGA GGAGGTTTCA GTAACCGTCA AGGCCTTCAT GACTGCAGAC 
CTTCCCAATG AGCTGATTGA ACTGCTGGAA AAGATCGTAC TCGACAACTC TGTCTTTAGC 
GAGCACAGAA ACCTCCAGAA CCTGCTCATT CTGACTGCCA TCAAGGCCGA CCGTACTCGG 
GTGATGGAGT ACATTAACAG ACTAGACAAC TACGACGCTC CGGACATTGC TAATATCGCC 
ATCAGCAATG AGCTATTTGA AGAAGCGTTT GCCATTTTCA AGAAGTTTGA TGTCAACACC 
TCTGCAATAC AGGTATTGAT AGAGCACATA GGGAACCTGG ACCGAGCCTA CGAGTTTGCA 
GAGCGCTGTA ACGAACCTGC GGTGTGGAGC CAGTTGGGCA GAGCTCAGCT GCACCGAGGC 
CTCGTCAAGG AGGCCATCGA CTCGTACATC AAAGCTGTCG ACCCTTCTGC GTACATGGAG 
GTGGTCAATG CAGCCAGCAA GAACAATAAT TGGGAAGATC TGGTGAAATT CCTCCAGATG 
GCTCGAAAGA AAGCCAGAGA GTCCTACGTG GAGACGGAGC TCATCTTCGC CTTGGCTAAA 
ACAAACCGTC TGGCCGAACT GGAGGAGTTC GTCAGTGGAC CCAACAATGC TCACATCCAG 
CAGGTGGGCG ATAGATGCTA CGAGGAAGGC ATGTACGAGG CAGCGAAGCT CTTGTATAAC 
AACGTTTCCA ACTTTGCTCG TCTCGCCTCG ACACTCGTAC ATCTCGGGGA GTACCAGGCG 
GCCGTGGACA GTGCCAGGAA AGCCAACAGC ACACGGACAT GGAAGGAGGT CTGCTTTGCG 
TGCGTGGATG GGGAGGAGTT CCGTCTGGCG CAAATCTGCG GCCTCCACAT CGTCATCCAC 
GCTGACGAGC TGGAAGACCT GATCAGCTAC TATCAGGACC GCGGATACTT CGAGGAACTC 
ATTGCGCTGC TGGAGGCGGC GCTGGGTTTG GAGCGGGCTC ACATGGGCAT GTTCACAGAG 
CTCGCCATCC TCTACTCCAA GTTCAAACCT CAGAAGATGA GGGAACACTT GGAGCTCTTC 
TGGTCCAGAG TCAATATTCC AAAGGTCCTC CGTGCAGCAG AGCAGTCTCA CCTCTGGGCA 
GAGCTGGTTT TCCTTTACGA TAAATACGAG GAGTACGACA ACGCAGTCCT CACGATGATG 
TCTCATCCGA CTGAAGCTTG GAAAGAAGGG CTGTTCAAAG ACATTATCCC AAAAGTGGCC 
AATGTGGAAC TGTACTATAA ATCCCTCTCC TTCTATCTGG ACTATAAACC GCTCCTACTC 
AACGACCTGC TGACCATCTT GTCTCCACGG CTTGATCACA GCCGAGCGGT CTCGTTTTTC 
TCAAAGATGA ACCAGCTGAA GCTGGTTAAG CCTTACCTGA GATCAGTCCA GAACCACAAC 
AACAAGTCTG TCAACGAGGC ACTCAACAAC CTGTTGACAG AGGAGGAAGA CTATCAGGGT 
CTGAGAGCGT CCATCGATGC CTACGACAAC TTTGATACCA TTGGCCTGGC GCAGAGATTA 
GAGAAGCACG ACCTGATCGA GTTCAGACGC ATTGCTGCTT ATCTTTATAA GGGTAACAAC 
CGCTGGAGAC AGAGTGTTGA GCTCTGCAAG AAGGACAAAC TCTACAAGGA TGCCATGCTG 
TACGCTGCAG AGTCGAAGGA TGCTGAGCTG GCAGAAAATC TGCTTCAGTG GTTCCTGGAG 
GAAGGCAGGA AAGAGTGCTT TGCTGCTTGC CTCTTTGCAT CCTATGACCT GCTGCATCCC 
GACGTAGTGC TGGAGCTGGC CTGGAGGCAC AATATCATGG ACTTCGCCAT GCCGTACTTC 
ATCCAGGTCA TGAGGGAGTA CCTCACAAAG ACACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029685577.1
CDS	402..5240
Translation	MAQILPIRFQEHLQLQNMGVNPANIGFSYLTMESDKFICIREKVGDQNQVVIVDMSDPTNPIRRPISADSAIMNPASKVIALKDAAKTLQIFNIEMKSKLKAHTMTEDVMFWKWISVNTVALVTDSAVYHWSMEGDSQPAKVFDRHASLAGCQIINYRTDQQQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFGDFKVEGNTKPSTLFCFAVRSQAGGKLHIIEVGQPAAGNQPFAKKAVDVFFPPEAQTDFPVAMQIGSKHGVIYLITKYGYIHLYDLESGVCIYMNRISAETIFVTAPHEATSGIIGVNKKGQVLSVCVEEENIVNYATNVLQNPDLALRIALRSNLAGAEEIFARKFNTLFSQGNYSEAAKVAASAPKGILRTAETIRKFQSVPAQPGQASPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKASDPTLALSVYLRANVPNKVIQCFAETGQFQKIVLYAKKVGYTPDWVFLLRNVMRVNPDQGLQFAQMLVQDEEPLANINQIVDVFMEGNLIQQCTSFLLDALKNNRPAEGHLQTRLLEMNLIHAPQVADAILGNQMFTHYDRAHVAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVEDSLECLRAMLSANIRQNLQLCVQVASKYHEQLGTQSLVELFESFKSYEGLFYFLGSIVNFSQEPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCAEDVIKNLIIVVRGQFSTDELVEEVEKRNRLKLLLPWLESRIHEGCEEPATHNALAKIYIDSNNTPERFLKENPFYDSAVVGKYCEKRDPHLACVAYERGQCDLDLIKVCNENSLFKSEARYLVHRKDPELWANVLEENNPFRRQLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFKKFDVNTSAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLGRAQLHRGLVKEAIDSYIKAVDPSAYMEVVNAASKNNNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFVSGPNNAHIQQVGDRCYEEGMYEAAKLLYNNVSNFARLASTLVHLGEYQAAVDSARKANSTRTWKEVCFACVDGEEFRLAQICGLHIVIHADELEDLISYYQDRGYFEELIALLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQSHLWAELVFLYDKYEEYDNAVLTMMSHPTEAWKEGLFKDIIPKVANVELYYKSLSFYLDYKPLLLNDLLTILSPRLDHSRAVSFFSKMNQLKLVKPYLRSVQNHNNKSVNEALNNLLTEEEDYQGLRASIDAYDNFDTIGLAQRLEKHDLIEFRRIAAYLYKGNNRWRQSVELCKKDKLYKDAMLYAAESKDAELAENLLQWFLEEGRKECFAACLFASYDLLHPDVVLELAWRHNIMDFAMPYFIQVMREYLTKTR

Target ORF information:

RefSeq Version	XM_029829717.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes clathrin heavy chain 1-like (LOC101077362), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029829717.1

ORF Insert Sequence:

ATGGCTCAGA TACTGCCGAT ACGATTTCAG GAACATCTGC AGTTGCAGAA CATGGGCGTG 
AACCCCGCCA ACATCGGGTT CAGCTATCTC ACCATGGAGT CGGACAAGTT TATCTGCATC 
AGAGAAAAGG TGGGGGACCA GAACCAGGTG GTGATTGTGG ACATGTCCGA CCCCACCAAC 
CCCATCAGGA GGCCCATCTC TGCCGACAGC GCCATCATGA ACCCTGCCAG CAAAGTCATC 
GCACTGAAAG ACGCTGCCAA GACCCTGCAG ATCTTTAACA TTGAGATGAA AAGTAAACTA 
AAGGCTCACA CCATGACAGA AGATGTGATG TTCTGGAAGT GGATATCGGT GAACACCGTC 
GCCCTGGTGA CTGATTCAGC CGTCTATCAC TGGAGCATGG AGGGAGACTC TCAGCCCGCT 
AAGGTTTTCG ACCGGCACGC CAGTCTGGCA GGCTGTCAGA TCATCAACTA CAGAACTGAT 
CAGCAACAGA AGTGGCTGCT TTTGATCGGG ATCTCCGCGC AGCAAAACCG CGTGGTTGGA 
GCCATGCAGC TGTATTCAGT CGACAGGAAA GTGTCCCAGC CCATCGAGGG ACACGCTGCT 
GCCTTTGGAG ACTTCAAAGT GGAGGGGAAC ACCAAACCTT CCACCCTCTT TTGCTTCGCC 
GTGCGCTCGC AAGCTGGGGG AAAGTTGCAC ATCATTGAAG TCGGTCAGCC AGCTGCAGGG 
AACCAGCCCT TTGCTAAGAA AGCCGTTGAT GTGTTTTTCC CTCCAGAGGC GCAGACAGAC 
TTTCCCGTAG CCATGCAGAT CGGAAGTAAA CATGGAGTCA TATATTTGAT CACAAAGTAT 
GGTTACATTC ACTTGTACGA CCTGGAGTCT GGAGTTTGCA TCTACATGAA TCGAATTAGC 
GCGGAGACCA TTTTTGTCAC GGCTCCACAT GAAGCCACCT CAGGAATCAT TGGGGTGAAC 
AAGAAGGGTC AGGTCCTCTC AGTCTGCGTT GAAGAGGAAA ACATCGTCAA CTACGCCACC 
AACGTTTTAC AGAACCCAGA CCTGGCGTTG AGGATAGCTT TAAGGTCCAA TCTCGCTGGG 
GCGGAGGAGA TTTTTGCGAG GAAGTTCAAC ACACTGTTCT CCCAGGGAAA TTATTCAGAG 
GCTGCAAAGG TTGCTGCCTC GGCACCTAAG GGAATTCTCC GCACTGCAGA GACCATTCGT 
AAGTTCCAGA GCGTCCCAGC TCAGCCAGGT CAGGCCTCCC CATTGTTGCA GTACTTTGGT 
ATTCTGCTGG ACCAGGGTCA GCTCAACAAG TTTGAGTCTT TGGAGCTGTG CCGGCCTGTC 
CTGCAGCAGG GCCGCAAGCA ACTGCTGGAG AAATGGCTGA AGGAGGACAA GTTGGAGTGC 
TCAGAAGAGC TGGGAGACCT GGTGAAGGCA TCGGATCCCA CTTTAGCTCT TAGCGTGTAC 
CTCAGAGCTA ACGTCCCCAA CAAGGTCATC CAGTGTTTTG CTGAAACTGG CCAGTTCCAG 
AAGATTGTGC TTTATGCCAA AAAGGTGGGC TACACCCCTG ACTGGGTTTT CCTCCTGAGA 
AACGTGATGC GTGTAAATCC AGACCAGGGC CTGCAGTTCG CCCAGATGCT TGTCCAGGAC 
GAGGAGCCGC TGGCCAACAT AAACCAGATT GTTGACGTCT TCATGGAGGG AAATCTGATC 
CAGCAGTGCA CGTCCTTCCT GTTGGACGCT CTGAAGAACA ACCGTCCCGC CGAAGGACAC 
TTACAGACTC GCCTGCTTGA GATGAACCTC ATACATGCCC CCCAGGTCGC GGATGCCATC 
CTTGGGAACC AGATGTTCAC CCATTATGAC CGGGCCCATG TGGCCCAGCT GTGCGAGAAG 
GCAGGTCTCC TGCAAAGAGC TCTTGAACAC TACACAGATC TGTACGACAT CAAACGGGCA 
GTGGTGCACA CGCATCTGCT CAACCCTGAG TGGCTGGTGA ACTTTTTTGG CTCGTTGTCA 
GTTGAAGACT CTTTGGAATG TCTGAGAGCC ATGTTGTCAG CTAACATCAG GCAGAACCTG 
CAGCTCTGCG TCCAGGTAGC ATCGAAGTAT CACGAGCAGC TGGGGACTCA GTCTTTAGTG 
GAACTCTTTG AATCCTTCAA GAGTTATGAG GGTTTGTTTT ACTTCCTTGG ATCGATTGTG 
AATTTCAGTC AGGAGCCTGA TGTTCACTTT AAGTACATCC AGGCTGCCTG CAAAACAGGT 
CAGATCAAGG AAGTGGAGAG AATCTGTCGA GAGAGCAACT GCTACGACCC AGAGAGAGTC 
AAAAACTTCC TCAAGGAGGC CAAGCTTACA GATCAGCTCC CTCTGATCAT CGTGTGTGAT 
CGCTTTGACT TTGTTCACGA CTTGGTGCTC TATCTCTACC GCAACAATCT CCAGAAATAC 
ATTGAAATCT ACGTGCAAAA GGTGAATCCC AGTCGTTTAC CGGTGGTTAT AGGTGGACTG 
TTGGATGTCG ACTGTGCTGA GGATGTGATC AAGAACCTGA TCATTGTGGT CAGAGGGCAG 
TTTTCCACTG ATGAGCTAGT AGAGGAAGTA GAAAAGAGGA ACAGGTTAAA GCTGTTGTTA 
CCATGGCTTG AGTCTCGCAT CCATGAAGGG TGCGAGGAAC CGGCCACTCA CAATGCGCTG 
GCCAAGATCT ATATTGATAG CAACAATACG CCCGAGCGTT TCCTGAAAGA GAACCCATTC 
TACGACAGCG CTGTTGTTGG AAAATACTGT GAGAAGAGAG ACCCTCACCT GGCCTGTGTA 
GCTTATGAGA GAGGACAATG TGACCTGGAC CTTATAAAGG TGTGCAATGA AAACTCCTTA 
TTCAAGAGTG AGGCTAGATA TCTGGTCCAT CGGAAAGACC CAGAGCTTTG GGCAAATGTG 
CTTGAAGAAA ACAACCCATT CAGACGACAA CTTATCGATC AGGTGGTGCA GACAGCCCTG 
TCAGAGACCC AGGACCCAGA GGAGGTTTCA GTAACCGTCA AGGCCTTCAT GACTGCAGAC 
CTTCCCAATG AGCTGATTGA ACTGCTGGAA AAGATCGTAC TCGACAACTC TGTCTTTAGC 
GAGCACAGAA ACCTCCAGAA CCTGCTCATT CTGACTGCCA TCAAGGCCGA CCGTACTCGG 
GTGATGGAGT ACATTAACAG ACTAGACAAC TACGACGCTC CGGACATTGC TAATATCGCC 
ATCAGCAATG AGCTATTTGA AGAAGCGTTT GCCATTTTCA AGAAGTTTGA TGTCAACACC 
TCTGCAATAC AGGTATTGAT AGAGCACATA GGGAACCTGG ACCGAGCCTA CGAGTTTGCA 
GAGCGCTGTA ACGAACCTGC GGTGTGGAGC CAGTTGGGCA GAGCTCAGCT GCACCGAGGC 
CTCGTCAAGG AGGCCATCGA CTCGTACATC AAAGCTGTCG ACCCTTCTGC GTACATGGAG 
GTGGTCAATG CAGCCAGCAA GAACAATAAT TGGGAAGATC TGGTGAAATT CCTCCAGATG 
GCTCGAAAGA AAGCCAGAGA GTCCTACGTG GAGACGGAGC TCATCTTCGC CTTGGCTAAA 
ACAAACCGTC TGGCCGAACT GGAGGAGTTC GTCAGTGGAC CCAACAATGC TCACATCCAG 
CAGGTGGGCG ATAGATGCTA CGAGGAAGGC ATGTACGAGG CAGCGAAGCT CTTGTATAAC 
AACGTTTCCA ACTTTGCTCG TCTCGCCTCG ACACTCGTAC ATCTCGGGGA GTACCAGGCG 
GCCGTGGACA GTGCCAGGAA AGCCAACAGC ACACGGACAT GGAAGGAGGT CTGCTTTGCG 
TGCGTGGATG GGGAGGAGTT CCGTCTGGCG CAAATCTGCG GCCTCCACAT CGTCATCCAC 
GCTGACGAGC TGGAAGACCT GATCAGCTAC TATCAGGACC GCGGATACTT CGAGGAACTC 
ATTGCGCTGC TGGAGGCGGC GCTGGGTTTG GAGCGGGCTC ACATGGGCAT GTTCACAGAG 
CTCGCCATCC TCTACTCCAA GTTCAAACCT CAGAAGATGA GGGAACACTT GGAGCTCTTC 
TGGTCCAGAG TCAATATTCC AAAGGTCCTC CGTGCAGCAG AGCAGTCTCA CCTCTGGGCA 
GAGCTGGTTT TCCTTTACGA TAAATACGAG GAGTACGACA ACGCAGTCCT CACGATGATG 
TCTCATCCGA CTGAAGCTTG GAAAGAAGGG CTGTTCAAAG ACATTATCCC AAAAGTGGCC 
AATGTGGAAC TGTACTATAA ATCCCTCTCC TTCTATCTGG ACTATAAACC GCTCCTACTC 
AACGACCTGC TGACCATCTT GTCTCCACGG CTTGATCACA GCCGAGCGGT CTCGTTTTTC 
TCAAAGATGA ACCAGCTGAA GCTGGTTAAG CCTTACCTGA GATCAGTCCA GAACCACAAC 
AACAAGTCTG TCAACGAGGC ACTCAACAAC CTGTTGACAG AGGAGGAAGA CTATCAGGGT 
CTGAGAGCGT CCATCGATGC CTACGACAAC TTTGATACCA TTGGCCTGGC GCAGAGATTA 
GAGAAGCACG ACCTGATCGA GTTCAGACGC ATTGCTGCTT ATCTTTATAA GGGTAACAAC 
CGCTGGAGAC AGAGTGTTGA GCTCTGCAAG AAGGACAAAC TCTACAAGGA TGCCATGCTG 
TACGCTGCAG AGTCGAAGGA TGCTGAGCTG GCAGAAAATC TGCTTCAGTG GTTCCTGGAG 
GAAGGCAGGA AAGAGTGCTT TGCTGCTTGC CTCTTTGCAT CCTATGACCT GCTGCATCCC 
GACGTAGTGC TGGAGCTGGC CTGGAGGCAC AATATCATGG ACTTCGCCAT GCCGTACTTC 
ATCCAGGTCA TGAGGGAGTA CCTCACAAAG ACACGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101077362 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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