LOC101074739 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101074739
Entrez Gene ID	101074739
Full Name	inositol 1,4,5-trisphosphate receptor type 1
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011613734.2	XP_011612036.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X2
XM_011613734.1	XP_011612036.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X2
XM_011613733.2	XP_011612035.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X1
XM_011613733.1	XP_011612035.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101074739 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101074739 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo28516	XM_011613733.1 Latest version!	Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo28517	XM_011613734.1 Latest version!	Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo56667	XM_011613733.2 Latest version!	Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OTo56668	XM_011613734.2 Latest version!	Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101074739 ( XM_011613733.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011612035.1 Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo28516
Clone ID Related Accession (Same CDS sequence)	XM_011613733.1
Accession Version	XM_011613733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA AGATGTCCAG CTTCCTGCAC ATTGGGGACA TCTGCTCGCT GTATGCAGAG 
GGCTCCACCA ATGGTTTCAT CAGCACTCTC GGCTTGGTGG ACGACCGCTG CGTCGTCCAG 
CCCGACGCCG GCGACCTCAA CAACCCCCCG AAGAAGTTCC GAGACTGCCT GTTCAGACTG 
TGTCCCATGA ACCGCTACTC GGCCCAGAAG CAGTTCTGGA AAGCGGCGAA GCCTGGAGGG 
AACACCGACG CTGTGCTCCT CAACAAGCTG CATCATGCCG CCGACCTGGA GAAGAAGCAG 
AACGACTCTG AGAACAAAAA GCTGCTTGGA ACAGTGATTC AGTACGGGAA CGTCATTCAG 
CTCCTCCACC TGAAGAGCAA CAAGTACCTG ACGGTGAACA AGCGTCTACC CGCTCTGCTT 
GAGAAGAATG CCATGAGGGT GATGCTGGAC ACAGCAGGAA ATGAGGGCTC CTGGTTCTAC 
ATCCAGCCCT TCTACAAACT GCGCTCCATC GGAGACAGCG TGGTGATTGG AGACAAAGTG 
GTTCTGAACC CAGTGAACGC CGGTCAGCCG CTGCACGCCA GCACGCACCA GCTGGTGGAT 
AATCCAGGAT GCAACGAGGT GAACTCGGTC AACTGTAACA CTAGCTGGAA AATCGTCCTG 
TTTATGAAAT GGAGCGATAA CCAAGAGATC GTTTTGAAAG GGGGTGACGT TGTGCGGTTA 
TTTCACGCAG AGCAGGAGAA GTTTCTCACG TGTGACGATC ACAGGAAAAA ACAGTATGTC 
TTCCTGCGCA CTACCGGACG TCAGTCTGCA ACGTCGGCCA CCAGCAGCAA AGCACTGTGG 
GAAGTCGAGG TTGTGCAGCA CGATCCGTGT CGAGGGGGGG CTGGCTACTG GAACAGTCTG 
TTCAGGTTCA AGCACCTGGC CACTGGGCAC TACCTGGCTG CAGAGGTGAA TCCAGAGTAT 
GAAGAGGAGG GTCCGGAATC ACGATCCCCT CTGGAGTTGG AGCAGGAAGC CCTCCGAGCC 
CGGCTGAGGA ACGTTCAGGA CAAGGTCATG TACACCCTTG TGTCTGTCCC TGACGGGAAT 
GACATCTCTT CCATTTTTGA GCTCGACCCT ACCACGCTAA GAGGGGGGGA CTCCCTGGTG 
CCCAGGAACT CGTATGTGCG TCTCAGACAC CTCTGCACCA ACACATGGGT TCACAGCACC 
AATCAGCCAA TCGACAAAGA GGAGGAGAAG CCTGTCATGT TGCGTATTGG CACCTCTACT 
CTAAAAGAGG ACAAGGAGGC CTTTGCCATT GTGCCCGTCT CCCCTGCTGA GGTGAGAGAC 
CTCGACTTTG CTAATGATGC CAGTAAAGTG CTTGCTTCTA TTGCTGGGAA GCTGGAAAAA 
GGCACCATCA CGCAGAACGA GAGGAGAGCG GTCACGAAGC TTCTGGAGGA CTTGGTGTTT 
TTTGTGGTGG ATATTCCCAA CAGTGGCCAG GATGTTCTGG AGATCATGGT CAACAAACCC 
AACAGGGAGC GTCAGAAGCT CATGAGGGAA CAGAATATTC TCAAACAGAT CTTCAAGCTG 
CTGCAGGCGC CCTTCACAGA CAGTGGGGAC GGACCCATGC TGAGACTGGA GGAGCTGGCC 
GACCAGCGCC ATGCCCCCTT CAGACACATC TGCCGACTGT GTTACCGCGT TTTGCGTCAC 
TCTCAGCAGG ACTACCGTAA AAATCAGGAA TATATAGCCA AACAGTTCCG CTTCATGCAG 
AAGCAGATCG GCTATGACGT GCTGGCCGAG GACACAATCA CAGCTCTGCT CCACAACAAC 
CGAAAGCTGC TGGAGAAGCA CATCACGGCC GCTGAGATCG ACACCTTCGT CAGCCTGGTT 
CGCAAGAACC GCGAGCCTAG GTTCCTGGAC TACTTGTCAG ACTTGTGCGT GTCGATGAAC 
AAGTCCATCC CCGTGACCCA GGAGCTCATC TGCAATGCAG TGCTGGATCA GGCTAACGCA 
GACATCCTCA TAGAGACTAA GCTGGTGCTG TCGAGATTTG AGATCGAGGG AGCGCCGCTC 
GGAGAAAACT CACTGGAGTC GGAGGAGGAC GAGGAGGAGG TGTGGCTGTT CTGGAAGGAC 
AATAATAATA AAGAAATCCG CAGTAAAAGC ATTCGGGAGC TGGCCCAGGA TGCCAAAGAG 
GGTCAGAAGG AGGACCAGGA AGTGATCGGT TACTACAGAT ACCAGTTGAA CCTGTTTGCC 
AGGATGTGTT TGGACCGGCA GTACCTGGCA ATCAACAAGA TTTCCGGCCA GTTGGACGTG 
GACCTAATCT TGCGCTGCAT GTCGGACGAA GACCTGCCCT TTGACCTGCG AGCCTCGTTC 
TGCCGCATGA TGCTGCACAT GCATGTGGAC CGCGATCCAC AGGAACAAGT CACACCGGTC 
AAATATGCAC GGCTCTGGTC TGAGATCCCC TCTGAGATCG CCATCGACGA CTATGACAAC 
GATGGGACGT CCAAGGACGA GATTAAAGAG CGCTTCTGTC AGACCATGGA GTTTGTCGAG 
AACTACCTGA GAGACGTTGT CTGTCAGAGT TTCCCGTTCG CAGATAAAGA AAAGAACAAG 
CTCACGTTTG AGGTGGTGAA CCTGGCAAGG AACCTGATTT ACTTCGGCTT CTACAACTTC 
AGTGACCTGC TGAGACTCAC CAAGATCCTG CTGGCCATTC TAGACTGCGT CCACGTCGGC 
ACCATTTTCG GTCTCAACAA GCTGGACAAA GAAGATGAGA GTAAAGGCAG CAATGTGATG 
AAGTCCATTC ACGGTGTGGG AGAGCTGATG TCCCACGTCG TCCTGAGAGG AGGGGGCTTT 
CTCCCCATGA CTTCAAGCAG CAGCACCAGC AGAGACACAG TTAAAACCCA GACTGAACCC 
GAGAAACAGG ACATTTTGGT CATGGACACC AAGCTGAAGA TAATAGAGAT CCTGCAGTTC 
ATCTTGAACG TGCGGCTGGA CTACAGGATC TCCTGTTTGC TGTCGATATT TAAGAGGGAA 
TTCGACGAGA GCAACTCCCA GAGCGAGCTA ACTGTGGGCG GAGCCGTGGA AGGGCCGAAC 
AACATGCCAG GAGCTTTGGA TTTTGAACAC ATCGAAGAAC AGGCAGAAGG CATATTTGGT 
GGAAGTGAAG AAAACACACC ACTGGACCTG GATGATCATG GCGGCCGTAC CTTTTTGAGG 
GTCCTGCTCC ACTTAACCAT GCATGATTAT CCACCACTGG TATCTAGAGC GCTCCAGTTG 
CTCTTCAGGC ATTTCAGCCA AAGGCAGGAG GTTTTGCAGG CTTTCAAGCA GGTCCAGCTG 
CTCGTCACCA GCCAGGATGT GGAAAATTAC AAACAAATCA AGTCGGACCT TGACCAGCTT 
CGCTCCATCG TGGAGAAGTC CGAACTCTGG GTGTACAAGC GGCAGGGCTC CGAGGAGGGG 
ATGGATGCAG GAGAGCCTGA GCACAAACTG GGTGATGCAG TGGGCACAGA CAAGACAAAG 
AAACCCGAAA GCACCAGCAG TTACAATTAC AGGGTGGTGA AGGAGATCCT GCTGCGGCTC 
AGCAAGTTGT GTGTCCAAGA AGGTCTGTCA GGCAGGAAGA GCAAGAAGCA GCAGCAGAGG 
CTGCTGAGGA ACATGGGGGC CCACGCCGTT GTTCTCGAGC TGCTTCAGAT CCCATACGAG 
AAGGGAGAAG ATTTGCGGAT GCAGGACATC ATGAAATTAG CCCACCAGTT TCTACAGAAC 
TTTTGTGCTG GGAACCAGCA GAACCAAGCC CTGCTGCACA AGCATATCAA TCTCTTCCTC 
AACCCCGGGA TTTTGGAAGC CATCACCATG CAGCACATCT TCATGAACAA CTTCCAGCTG 
TGCAGCGAGA TAAACGAGCG CGTGGTTCAG CACTTTGTCC ACTGCATCGA GACGCACGGG 
CGCAACGTTC AGTATCTGAA ATTCCTTCAG ACTATCGTCA AAGCGGAGAA CAAGTTCATC 
AAAAAGTGTC AGGACATCGT CATGGCTGAG CTCGTAAACG CCGGCGAGGA CGTTTTGGTC 
TTCTACAACG ACCGCGCCTC CTTCCAGACG CTGGTGCAGA TGATGCGATC GGAGCGGGAC 
CGCATGGATG AAAACAGTCC CCTGATGTAC CACATCCACC TAGTAGAGCT ACTGGCCGTG 
TGCACGGAGG GCAAGAATGT CTACACAGAG ATCAAGTGCA ACTCCTTGCT ACCTCTAGAT 
GACATCGTGC GGGTGGTGAC GCATGAGGAC TGTATTCCCG AGGTGAAGAT CGCTTACATC 
AACTTCCTAA ACCACTGCTA TGTCGACACG GAAGTGGAGA TGAAAGAGAT CTACACCTCC 
AACCACATGT GGAAACTCTT TGAGAACTTT CTGGTCGACA TCTGCCGGGT GTGTAACAGC 
ACGAGCGAGC GCAAGCACGC CGACACCATT CTGGAGCGCT ACGTGACGGA GACGGTCATG 
AGCATCGTAA CGACGTTCTT CAGCTCGCCA TTCTCGGACC AGAGCACCAG CCTGCAGACC 
AGACAGCCTG TCTTTGTTCA GCTATTGCAG GCGATTTTCA GGGTGTACCA CTGTAACTGG 
CTGATCCCAG TTCAGAAGGG CTCTGTAGAG AGCTGCATTA AGGTGCTCTC TGATGTTGCC 
AAAGGTCGAG CAATAGCCAT CCCGGTGGAC CTGGACAACC AGGTGCACAA CCTTTTTGTG 
AAATCCAACA ATATTGTTCA GAAGACGGCC ATGAGCTGGA GACTTTCGGC CCGCAATGCT 
GCCCGCAGGG ACTCGATGGT GACTGCCTCA CGGGACTACA GGAACATCAT AGAGAGACTA 
CAGGACATCG TGTCAGCACT GGAGGACCGT CTGCGCCCGC TGGTCCAGGC CGAGTTATCG 
GTCCTGGTGG ACGTGCTGCA TCGGCCTGAG CTGCTCTTCC CTGAAAACAC CGACTCGCGC 
AAGAAGTGCG AGAGCGGAGG TTTTATCTGC AAGCTGATCA AACATACCAA GCAGCTGCTG 
GAGGAAAATG AAGAGAGGCT GTGCATCAAA GTCCTGCAAA CTCTGCGAGA GATGATGACA 
AAAGACCGAG GCTATGGAGA GAAGCTCAGG GCCTTTGATG ATGAAATGGA TATGCCCAAG 
CAGCTGGATC AGAACCAGCC CGAGAAACTG TTGGAGGACC AGAAGAGGGG CGAGGCGCTC 
AGACAGATTC TGGTGAACCG TTACTATGGC AACTTCCACC GGAGCGGCGG ACGCAGGGAC 
AGCCTGACCT TTGCCAACGG CCCGCTCAGT CCGATCGGGC CTGGCAAAAG CCAATCAGGC 
TTGGGGGGTC CTGGGGCCTT GAGCCGTGGG GAGATGAGCC TGGCGGAGGT TCAGTGTCAC 
TTGGACAAGG AGGGAGCCTC GGACCTGGTG ATCGATCTCA TCATGAACAC CACAAGCGAC 
CGAGTCTTCC AAGAAAGCAT CCTGCTGGCT ATCGCACTGC TCGAAGGTGG AAACACAACC 
ATCCAGCGGT CATTCTTTTG TCGCCTGACT GAAGACAAGA AGTCAGAGAA GTTCTTCCGG 
GTCTTCTACG ACCGTATGAA GCTGGCTCAG CTGGAGATCA AAGCCACTGT GACGGTAAAC 
ACCAGCGATT TAGGAAACAG GAAGCGAGAA GATGACAACC AGGACAAAGA CGGCCCTGTT 
CGTAAAAAAG CCAGAGAATC TTCTGTGGTC ATGACGGAGG ATGTGCGAGA ACAGCTGTTA 
GAAGCATCTT CTGCCACCAA GAAAGCCTTC AACTCTTATC GGCGTGAAGC AGACCCCGAG 
GACCACTTCA ACGTAGTGGA CGGCCAGTCC AGCGCGGGAG ACAAGAACCA GGATGAAGGA 
GAAATGAGCT TTGTTATCGT CATCATGCAG CCGATCCTTC GCTTCCTTCA GCTGCTGTGT 
GAGAACCACA ACCGGGATCT GCAGAACTTC CTCCGCTGTC AAAATAACAA GAACAACTAC 
AACCTTGTGT GCGAGACCCT TCAGTTCTTG GACTGTATCT GTGGCAGCAC CACGGGGGGC 
CTCGGCCTAC TGGGCCTTTA CATCAATGAG AAGAATGTGG CCCTCATTAA CCAAACCCTG 
GAGAGTCTGA CTGAGTACTG TCAGGGCCCC TGCCATGAAA ACCAGAATTG TATCGCCACC 
CATGAGTCCA ATGGAATTGA CATTATCATC GCCCTGATTC TTAACGACAT CAACCCACTT 
GGGAAGAAGC GAATGGACCT GGTGCTGGAG CTGAAGAACA ATGCGTCCAA GCTGCTGCTG 
GCAATCATGG AGAGTCGACA TGACAGTGAG AATGCAGAGA GGATCCTGTA TAATATGAGA 
CCCAAAGAGC TGGTGGAGGT GATAAAGAAG GCTTACTTAC AAGGAGAGGT GGAGATCGAG 
GACACAAAAG AGGAGGAAGA GAATGAAGAG GAAGATCACG ACGCTGCTTC TCCCCGAAAC 
GTTGGACACA ACATCTATAT TTTAGCTCAC CAGTTGGCGC GTCACAATAA GGAGCTGGCG 
GTGATGTTAA AGCCAGGCGT GGCCGTTGGG GAGGGAGATG AGGCCCTGGA GTGTTACGCC 
AAACACACCG CTCAGATCGA GATTGTGCGT CAGGACCGTA CCATGGAGGA GATCGTGTTC 
CCAGTGCCAA ACATCTGCGA GTTCCTGACC ACCGAATCCA AGCTGCGCGT CTACTATACC 
ACAGAGAGAG ATGAGCAGGG CAGCAAGATC AACGACTTCT TCTTGCGAGC GGAAGACCTC 
TTTAATGAGA TGAACTGGCA GAAGAAGCTT AGAGCCCAGC CAGTGTTGTA CTGGTGCTCC 
AGGAACATGT CGGTCTGGAG TAACATTTGC TTTAACCTGG CCTTACTCAT GAACCTGCTG 
GTCTGCCTCT TCTACCCGCT GGAAGGGGTG CAAGGAGTTA TGCTGGATCC CCACCTGTCT 
GCCCTGCTGT GGATGGGGGT CGTTGCGGCC CTGGTGATTG TCCTCGTCAT GCCTCAGCCG 
CTGAGCGTCC GCGCCCTCGT CATCATCACC ATCCTCCGCC TCATCTTCTC GGTCGGCCTT 
GAGCCCACCC TCTTCCTTCT GGGCGCCTTG AATGTGTGCA ATAAAATCAT CTTCATGATC 
AGCTTCGTGG GGAACCGGGG AACCTTTACG CGCGGCTACA AAGCCATGGT GCTGGACTTT 
GAGTTCCTCT ACCACCTCAT TTACCTCATC ATTTGCTGTC TAGGAGTGTT CGTCCACGTC 
TTCTTCTACA GCCTGCTGCT TTTTGACCTC GTGTACCGAG AGGAGACGCT GCTGAACGTG 
ATCAAGAGTG TGACCCGCAA CGGCCGCTCC ATCGTGCTGA CCGCCGTGCT GGCTCTCATC 
CTGGTCTACC TGTTCTCCAT CGTCGGCTAC ATTTTCTTCA AGGATGACTT CATCCTGGAG 
GTGGACCGCG TCCCCAACGC AACTACGAAA GGTGGAGGCA GTCTGGCCAG CGTGTTTGTT 
CCCGCTGGAA TGTGTCAGGG AGGGACTGAT CAGAACTGCA CCGCAAGGGC CCAGCGGGAA 
GGTGATCGGG ACCGCGTCGG TGGTGACGGC GACGGGGACA AGGACGAGGA CGTGGAGCGG 
ACGTGCGACT CCCTGCTGAT GTGCATCGTT ACCGTCCTGA GTCACGGCTT GAGGAGCGGA 
GGAGGAGTCG GTGATGTTCT TCGCAAGCCT TCCAAGGAGG AGCCTCTGTT TGCCGCCAGA 
GTGATATACG ACTTGCTCTT CTTCTTCATG GTCATCATCA TCGTCCTCAA CCTCATCTTC 
GGTGTCATCA TTGACACCTT CGCTGACCTG AGGAGTGAGA AACAGAAGAA AGAGGAGGTT 
CTGAAAACGA CCTGCTTCAT CTGCGGTCTG GAGCGGGACA AGTTCGACAA CAAGACCGTG 
ACGTTTGAGG AGCACATCAA GGAGGAACAC AACATGTGGC ACTACCTGTT CTTTATCGTG 
CTCGTGAAAG TTAAAGACTC CACTGAGTAC ACAGGCCCCG AGAGCTACGT GGCTGAGATG 
ATAAAGGAGC ACAACCTGGA CTGGTTCCCA CGGATGCGTG CCATGTCTCT GGTGAGCAGC 
GACGCCGAGG GGGAGCAGAA TGAAATCAGG AACCTGCAGG AGAAGCTGGA GTCGACCATG 
AGGCTGGTGG CCAACCTGTC TGGACAGCTG AGTGAGCTCA AGGATCAGAT GACAGAGCAG 
AGGAAACACA AGCAACGGAT CGGTCTCCTT GGACACCCCC CCCACATGAA CATCAATCCA 
CAGCAACCAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011612035.1
CDS	246..8480
Translation	MSDKMSSFLHIGDICSLYAEGSTNGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGNTDAVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVMLDTAGNEGSWFYIQPFYKLRSIGDSVVIGDKVVLNPVNAGQPLHASTHQLVDNPGCNEVNSVNCNTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDDHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPEYEEEGPESRSPLELEQEALRARLRNVQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSTLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAGKLEKGTITQNERRAVTKLLEDLVFFVVDIPNSGQDVLEIMVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRHSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDQANADILIETKLVLSRFEIEGAPLGENSLESEEDEEEVWLFWKDNNNKEIRSKSIRELAQDAKEGQKEDQEVIGYYRYQLNLFARMCLDRQYLAINKISGQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSEIAIDDYDNDGTSKDEIKERFCQTMEFVENYLRDVVCQSFPFADKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHVGTIFGLNKLDKEDESKGSNVMKSIHGVGELMSHVVLRGGGFLPMTSSSSTSRDTVKTQTEPEKQDILVMDTKLKIIEILQFILNVRLDYRISCLLSIFKREFDESNSQSELTVGGAVEGPNNMPGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALQLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSEEGMDAGEPEHKLGDAVGTDKTKKPESTSSYNYRVVKEILLRLSKLCVQEGLSGRKSKKQQQRLLRNMGAHAVVLELLQIPYEKGEDLRMQDIMKLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAITMQHIFMNNFQLCSEINERVVQHFVHCIETHGRNVQYLKFLQTIVKAENKFIKKCQDIVMAELVNAGEDVLVFYNDRASFQTLVQMMRSERDRMDENSPLMYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKIAYINFLNHCYVDTEVEMKEIYTSNHMWKLFENFLVDICRVCNSTSERKHADTILERYVTETVMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQAIFRVYHCNWLIPVQKGSVESCIKVLSDVAKGRAIAIPVDLDNQVHNLFVKSNNIVQKTAMSWRLSARNAARRDSMVTASRDYRNIIERLQDIVSALEDRLRPLVQAELSVLVDVLHRPELLFPENTDSRKKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLRAFDDEMDMPKQLDQNQPEKLLEDQKRGEALRQILVNRYYGNFHRSGGRRDSLTFANGPLSPIGPGKSQSGLGGPGALSRGEMSLAEVQCHLDKEGASDLVIDLIMNTTSDRVFQESILLAIALLEGGNTTIQRSFFCRLTEDKKSEKFFRVFYDRMKLAQLEIKATVTVNTSDLGNRKREDDNQDKDGPVRKKARESSVVMTEDVREQLLEASSATKKAFNSYRREADPEDHFNVVDGQSSAGDKNQDEGEMSFVIVIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINEKNVALINQTLESLTEYCQGPCHENQNCIATHESNGIDIIIALILNDINPLGKKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVIKKAYLQGEVEIEDTKEEEENEEEDHDAASPRNVGHNIYILAHQLARHNKELAVMLKPGVAVGEGDEALECYAKHTAQIEIVRQDRTMEEIVFPVPNICEFLTTESKLRVYYTTERDEQGSKINDFFLRAEDLFNEMNWQKKLRAQPVLYWCSRNMSVWSNICFNLALLMNLLVCLFYPLEGVQGVMLDPHLSALLWMGVVAALVIVLVMPQPLSVRALVIITILRLIFSVGLEPTLFLLGALNVCNKIIFMISFVGNRGTFTRGYKAMVLDFEFLYHLIYLIICCLGVFVHVFFYSLLLFDLVYREETLLNVIKSVTRNGRSIVLTAVLALILVYLFSIVGYIFFKDDFILEVDRVPNATTKGGGSLASVFVPAGMCQGGTDQNCTARAQREGDRDRVGGDGDGDKDEDVERTCDSLLMCIVTVLSHGLRSGGGVGDVLRKPSKEEPLFAARVIYDLLFFFMVIIIVLNLIFGVIIDTFADLRSEKQKKEEVLKTTCFICGLERDKFDNKTVTFEEHIKEEHNMWHYLFFIVLVKVKDSTEYTGPESYVAEMIKEHNLDWFPRMRAMSLVSSDAEGEQNEIRNLQEKLESTMRLVANLSGQLSELKDQMTEQRKHKQRIGLLGHPPHMNINPQQPA

Target ORF information:

RefSeq Version	XM_011613733.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011613733.1

ORF Insert Sequence:

ATGTCGGACA AGATGTCCAG CTTCCTGCAC ATTGGGGACA TCTGCTCGCT GTATGCAGAG 
GGCTCCACCA ATGGTTTCAT CAGCACTCTC GGCTTGGTGG ACGACCGCTG CGTCGTCCAG 
CCCGACGCCG GCGACCTCAA CAACCCCCCG AAGAAGTTCC GAGACTGCCT GTTCAGACTG 
TGTCCCATGA ACCGCTACTC GGCCCAGAAG CAGTTCTGGA AAGCGGCGAA GCCTGGAGGG 
AACACCGACG CTGTGCTCCT CAACAAGCTG CATCATGCCG CCGACCTGGA GAAGAAGCAG 
AACGACTCTG AGAACAAAAA GCTGCTTGGA ACAGTGATTC AGTACGGGAA CGTCATTCAG 
CTCCTCCACC TGAAGAGCAA CAAGTACCTG ACGGTGAACA AGCGTCTACC CGCTCTGCTT 
GAGAAGAATG CCATGAGGGT GATGCTGGAC ACAGCAGGAA ATGAGGGCTC CTGGTTCTAC 
ATCCAGCCCT TCTACAAACT GCGCTCCATC GGAGACAGCG TGGTGATTGG AGACAAAGTG 
GTTCTGAACC CAGTGAACGC CGGTCAGCCG CTGCACGCCA GCACGCACCA GCTGGTGGAT 
AATCCAGGAT GCAACGAGGT GAACTCGGTC AACTGTAACA CTAGCTGGAA AATCGTCCTG 
TTTATGAAAT GGAGCGATAA CCAAGAGATC GTTTTGAAAG GGGGTGACGT TGTGCGGTTA 
TTTCACGCAG AGCAGGAGAA GTTTCTCACG TGTGACGATC ACAGGAAAAA ACAGTATGTC 
TTCCTGCGCA CTACCGGACG TCAGTCTGCA ACGTCGGCCA CCAGCAGCAA AGCACTGTGG 
GAAGTCGAGG TTGTGCAGCA CGATCCGTGT CGAGGGGGGG CTGGCTACTG GAACAGTCTG 
TTCAGGTTCA AGCACCTGGC CACTGGGCAC TACCTGGCTG CAGAGGTGAA TCCAGAGTAT 
GAAGAGGAGG GTCCGGAATC ACGATCCCCT CTGGAGTTGG AGCAGGAAGC CCTCCGAGCC 
CGGCTGAGGA ACGTTCAGGA CAAGGTCATG TACACCCTTG TGTCTGTCCC TGACGGGAAT 
GACATCTCTT CCATTTTTGA GCTCGACCCT ACCACGCTAA GAGGGGGGGA CTCCCTGGTG 
CCCAGGAACT CGTATGTGCG TCTCAGACAC CTCTGCACCA ACACATGGGT TCACAGCACC 
AATCAGCCAA TCGACAAAGA GGAGGAGAAG CCTGTCATGT TGCGTATTGG CACCTCTACT 
CTAAAAGAGG ACAAGGAGGC CTTTGCCATT GTGCCCGTCT CCCCTGCTGA GGTGAGAGAC 
CTCGACTTTG CTAATGATGC CAGTAAAGTG CTTGCTTCTA TTGCTGGGAA GCTGGAAAAA 
GGCACCATCA CGCAGAACGA GAGGAGAGCG GTCACGAAGC TTCTGGAGGA CTTGGTGTTT 
TTTGTGGTGG ATATTCCCAA CAGTGGCCAG GATGTTCTGG AGATCATGGT CAACAAACCC 
AACAGGGAGC GTCAGAAGCT CATGAGGGAA CAGAATATTC TCAAACAGAT CTTCAAGCTG 
CTGCAGGCGC CCTTCACAGA CAGTGGGGAC GGACCCATGC TGAGACTGGA GGAGCTGGCC 
GACCAGCGCC ATGCCCCCTT CAGACACATC TGCCGACTGT GTTACCGCGT TTTGCGTCAC 
TCTCAGCAGG ACTACCGTAA AAATCAGGAA TATATAGCCA AACAGTTCCG CTTCATGCAG 
AAGCAGATCG GCTATGACGT GCTGGCCGAG GACACAATCA CAGCTCTGCT CCACAACAAC 
CGAAAGCTGC TGGAGAAGCA CATCACGGCC GCTGAGATCG ACACCTTCGT CAGCCTGGTT 
CGCAAGAACC GCGAGCCTAG GTTCCTGGAC TACTTGTCAG ACTTGTGCGT GTCGATGAAC 
AAGTCCATCC CCGTGACCCA GGAGCTCATC TGCAATGCAG TGCTGGATCA GGCTAACGCA 
GACATCCTCA TAGAGACTAA GCTGGTGCTG TCGAGATTTG AGATCGAGGG AGCGCCGCTC 
GGAGAAAACT CACTGGAGTC GGAGGAGGAC GAGGAGGAGG TGTGGCTGTT CTGGAAGGAC 
AATAATAATA AAGAAATCCG CAGTAAAAGC ATTCGGGAGC TGGCCCAGGA TGCCAAAGAG 
GGTCAGAAGG AGGACCAGGA AGTGATCGGT TACTACAGAT ACCAGTTGAA CCTGTTTGCC 
AGGATGTGTT TGGACCGGCA GTACCTGGCA ATCAACAAGA TTTCCGGCCA GTTGGACGTG 
GACCTAATCT TGCGCTGCAT GTCGGACGAA GACCTGCCCT TTGACCTGCG AGCCTCGTTC 
TGCCGCATGA TGCTGCACAT GCATGTGGAC CGCGATCCAC AGGAACAAGT CACACCGGTC 
AAATATGCAC GGCTCTGGTC TGAGATCCCC TCTGAGATCG CCATCGACGA CTATGACAAC 
GATGGGACGT CCAAGGACGA GATTAAAGAG CGCTTCTGTC AGACCATGGA GTTTGTCGAG 
AACTACCTGA GAGACGTTGT CTGTCAGAGT TTCCCGTTCG CAGATAAAGA AAAGAACAAG 
CTCACGTTTG AGGTGGTGAA CCTGGCAAGG AACCTGATTT ACTTCGGCTT CTACAACTTC 
AGTGACCTGC TGAGACTCAC CAAGATCCTG CTGGCCATTC TAGACTGCGT CCACGTCGGC 
ACCATTTTCG GTCTCAACAA GCTGGACAAA GAAGATGAGA GTAAAGGCAG CAATGTGATG 
AAGTCCATTC ACGGTGTGGG AGAGCTGATG TCCCACGTCG TCCTGAGAGG AGGGGGCTTT 
CTCCCCATGA CTTCAAGCAG CAGCACCAGC AGAGACACAG TTAAAACCCA GACTGAACCC 
GAGAAACAGG ACATTTTGGT CATGGACACC AAGCTGAAGA TAATAGAGAT CCTGCAGTTC 
ATCTTGAACG TGCGGCTGGA CTACAGGATC TCCTGTTTGC TGTCGATATT TAAGAGGGAA 
TTCGACGAGA GCAACTCCCA GAGCGAGCTA ACTGTGGGCG GAGCCGTGGA AGGGCCGAAC 
AACATGCCAG GAGCTTTGGA TTTTGAACAC ATCGAAGAAC AGGCAGAAGG CATATTTGGT 
GGAAGTGAAG AAAACACACC ACTGGACCTG GATGATCATG GCGGCCGTAC CTTTTTGAGG 
GTCCTGCTCC ACTTAACCAT GCATGATTAT CCACCACTGG TATCTAGAGC GCTCCAGTTG 
CTCTTCAGGC ATTTCAGCCA AAGGCAGGAG GTTTTGCAGG CTTTCAAGCA GGTCCAGCTG 
CTCGTCACCA GCCAGGATGT GGAAAATTAC AAACAAATCA AGTCGGACCT TGACCAGCTT 
CGCTCCATCG TGGAGAAGTC CGAACTCTGG GTGTACAAGC GGCAGGGCTC CGAGGAGGGG 
ATGGATGCAG GAGAGCCTGA GCACAAACTG GGTGATGCAG TGGGCACAGA CAAGACAAAG 
AAACCCGAAA GCACCAGCAG TTACAATTAC AGGGTGGTGA AGGAGATCCT GCTGCGGCTC 
AGCAAGTTGT GTGTCCAAGA AGGTCTGTCA GGCAGGAAGA GCAAGAAGCA GCAGCAGAGG 
CTGCTGAGGA ACATGGGGGC CCACGCCGTT GTTCTCGAGC TGCTTCAGAT CCCATACGAG 
AAGGGAGAAG ATTTGCGGAT GCAGGACATC ATGAAATTAG CCCACCAGTT TCTACAGAAC 
TTTTGTGCTG GGAACCAGCA GAACCAAGCC CTGCTGCACA AGCATATCAA TCTCTTCCTC 
AACCCCGGGA TTTTGGAAGC CATCACCATG CAGCACATCT TCATGAACAA CTTCCAGCTG 
TGCAGCGAGA TAAACGAGCG CGTGGTTCAG CACTTTGTCC ACTGCATCGA GACGCACGGG 
CGCAACGTTC AGTATCTGAA ATTCCTTCAG ACTATCGTCA AAGCGGAGAA CAAGTTCATC 
AAAAAGTGTC AGGACATCGT CATGGCTGAG CTCGTAAACG CCGGCGAGGA CGTTTTGGTC 
TTCTACAACG ACCGCGCCTC CTTCCAGACG CTGGTGCAGA TGATGCGATC GGAGCGGGAC 
CGCATGGATG AAAACAGTCC CCTGATGTAC CACATCCACC TAGTAGAGCT ACTGGCCGTG 
TGCACGGAGG GCAAGAATGT CTACACAGAG ATCAAGTGCA ACTCCTTGCT ACCTCTAGAT 
GACATCGTGC GGGTGGTGAC GCATGAGGAC TGTATTCCCG AGGTGAAGAT CGCTTACATC 
AACTTCCTAA ACCACTGCTA TGTCGACACG GAAGTGGAGA TGAAAGAGAT CTACACCTCC 
AACCACATGT GGAAACTCTT TGAGAACTTT CTGGTCGACA TCTGCCGGGT GTGTAACAGC 
ACGAGCGAGC GCAAGCACGC CGACACCATT CTGGAGCGCT ACGTGACGGA GACGGTCATG 
AGCATCGTAA CGACGTTCTT CAGCTCGCCA TTCTCGGACC AGAGCACCAG CCTGCAGACC 
AGACAGCCTG TCTTTGTTCA GCTATTGCAG GCGATTTTCA GGGTGTACCA CTGTAACTGG 
CTGATCCCAG TTCAGAAGGG CTCTGTAGAG AGCTGCATTA AGGTGCTCTC TGATGTTGCC 
AAAGGTCGAG CAATAGCCAT CCCGGTGGAC CTGGACAACC AGGTGCACAA CCTTTTTGTG 
AAATCCAACA ATATTGTTCA GAAGACGGCC ATGAGCTGGA GACTTTCGGC CCGCAATGCT 
GCCCGCAGGG ACTCGATGGT GACTGCCTCA CGGGACTACA GGAACATCAT AGAGAGACTA 
CAGGACATCG TGTCAGCACT GGAGGACCGT CTGCGCCCGC TGGTCCAGGC CGAGTTATCG 
GTCCTGGTGG ACGTGCTGCA TCGGCCTGAG CTGCTCTTCC CTGAAAACAC CGACTCGCGC 
AAGAAGTGCG AGAGCGGAGG TTTTATCTGC AAGCTGATCA AACATACCAA GCAGCTGCTG 
GAGGAAAATG AAGAGAGGCT GTGCATCAAA GTCCTGCAAA CTCTGCGAGA GATGATGACA 
AAAGACCGAG GCTATGGAGA GAAGCTCAGG GCCTTTGATG ATGAAATGGA TATGCCCAAG 
CAGCTGGATC AGAACCAGCC CGAGAAACTG TTGGAGGACC AGAAGAGGGG CGAGGCGCTC 
AGACAGATTC TGGTGAACCG TTACTATGGC AACTTCCACC GGAGCGGCGG ACGCAGGGAC 
AGCCTGACCT TTGCCAACGG CCCGCTCAGT CCGATCGGGC CTGGCAAAAG CCAATCAGGC 
TTGGGGGGTC CTGGGGCCTT GAGCCGTGGG GAGATGAGCC TGGCGGAGGT TCAGTGTCAC 
TTGGACAAGG AGGGAGCCTC GGACCTGGTG ATCGATCTCA TCATGAACAC CACAAGCGAC 
CGAGTCTTCC AAGAAAGCAT CCTGCTGGCT ATCGCACTGC TCGAAGGTGG AAACACAACC 
ATCCAGCGGT CATTCTTTTG TCGCCTGACT GAAGACAAGA AGTCAGAGAA GTTCTTCCGG 
GTCTTCTACG ACCGTATGAA GCTGGCTCAG CTGGAGATCA AAGCCACTGT GACGGTAAAC 
ACCAGCGATT TAGGAAACAG GAAGCGAGAA GATGACAACC AGGACAAAGA CGGCCCTGTT 
CGTAAAAAAG CCAGAGAATC TTCTGTGGTC ATGACGGAGG ATGTGCGAGA ACAGCTGTTA 
GAAGCATCTT CTGCCACCAA GAAAGCCTTC AACTCTTATC GGCGTGAAGC AGACCCCGAG 
GACCACTTCA ACGTAGTGGA CGGCCAGTCC AGCGCGGGAG ACAAGAACCA GGATGAAGGA 
GAAATGAGCT TTGTTATCGT CATCATGCAG CCGATCCTTC GCTTCCTTCA GCTGCTGTGT 
GAGAACCACA ACCGGGATCT GCAGAACTTC CTCCGCTGTC AAAATAACAA GAACAACTAC 
AACCTTGTGT GCGAGACCCT TCAGTTCTTG GACTGTATCT GTGGCAGCAC CACGGGGGGC 
CTCGGCCTAC TGGGCCTTTA CATCAATGAG AAGAATGTGG CCCTCATTAA CCAAACCCTG 
GAGAGTCTGA CTGAGTACTG TCAGGGCCCC TGCCATGAAA ACCAGAATTG TATCGCCACC 
CATGAGTCCA ATGGAATTGA CATTATCATC GCCCTGATTC TTAACGACAT CAACCCACTT 
GGGAAGAAGC GAATGGACCT GGTGCTGGAG CTGAAGAACA ATGCGTCCAA GCTGCTGCTG 
GCAATCATGG AGAGTCGACA TGACAGTGAG AATGCAGAGA GGATCCTGTA TAATATGAGA 
CCCAAAGAGC TGGTGGAGGT GATAAAGAAG GCTTACTTAC AAGGAGAGGT GGAGATCGAG 
GACACAAAAG AGGAGGAAGA GAATGAAGAG GAAGATCACG ACGCTGCTTC TCCCCGAAAC 
GTTGGACACA ACATCTATAT TTTAGCTCAC CAGTTGGCGC GTCACAATAA GGAGCTGGCG 
GTGATGTTAA AGCCAGGCGT GGCCGTTGGG GAGGGAGATG AGGCCCTGGA GTGTTACGCC 
AAACACACCG CTCAGATCGA GATTGTGCGT CAGGACCGTA CCATGGAGGA GATCGTGTTC 
CCAGTGCCAA ACATCTGCGA GTTCCTGACC ACCGAATCCA AGCTGCGCGT CTACTATACC 
ACAGAGAGAG ATGAGCAGGG CAGCAAGATC AACGACTTCT TCTTGCGAGC GGAAGACCTC 
TTTAATGAGA TGAACTGGCA GAAGAAGCTT AGAGCCCAGC CAGTGTTGTA CTGGTGCTCC 
AGGAACATGT CGGTCTGGAG TAACATTTGC TTTAACCTGG CCTTACTCAT GAACCTGCTG 
GTCTGCCTCT TCTACCCGCT GGAAGGGGTG CAAGGAGTTA TGCTGGATCC CCACCTGTCT 
GCCCTGCTGT GGATGGGGGT CGTTGCGGCC CTGGTGATTG TCCTCGTCAT GCCTCAGCCG 
CTGAGCGTCC GCGCCCTCGT CATCATCACC ATCCTCCGCC TCATCTTCTC GGTCGGCCTT 
GAGCCCACCC TCTTCCTTCT GGGCGCCTTG AATGTGTGCA ATAAAATCAT CTTCATGATC 
AGCTTCGTGG GGAACCGGGG AACCTTTACG CGCGGCTACA AAGCCATGGT GCTGGACTTT 
GAGTTCCTCT ACCACCTCAT TTACCTCATC ATTTGCTGTC TAGGAGTGTT CGTCCACGTC 
TTCTTCTACA GCCTGCTGCT TTTTGACCTC GTGTACCGAG AGGAGACGCT GCTGAACGTG 
ATCAAGAGTG TGACCCGCAA CGGCCGCTCC ATCGTGCTGA CCGCCGTGCT GGCTCTCATC 
CTGGTCTACC TGTTCTCCAT CGTCGGCTAC ATTTTCTTCA AGGATGACTT CATCCTGGAG 
GTGGACCGCG TCCCCAACGC AACTACGAAA GGTGGAGGCA GTCTGGCCAG CGTGTTTGTT 
CCCGCTGGAA TGTGTCAGGG AGGGACTGAT CAGAACTGCA CCGCAAGGGC CCAGCGGGAA 
GGTGATCGGG ACCGCGTCGG TGGTGACGGC GACGGGGACA AGGACGAGGA CGTGGAGCGG 
ACGTGCGACT CCCTGCTGAT GTGCATCGTT ACCGTCCTGA GTCACGGCTT GAGGAGCGGA 
GGAGGAGTCG GTGATGTTCT TCGCAAGCCT TCCAAGGAGG AGCCTCTGTT TGCCGCCAGA 
GTGATATACG ACTTGCTCTT CTTCTTCATG GTCATCATCA TCGTCCTCAA CCTCATCTTC 
GGTGTCATCA TTGACACCTT CGCTGACCTG AGGAGTGAGA AACAGAAGAA AGAGGAGGTT 
CTGAAAACGA CCTGCTTCAT CTGCGGTCTG GAGCGGGACA AGTTCGACAA CAAGACCGTG 
ACGTTTGAGG AGCACATCAA GGAGGAACAC AACATGTGGC ACTACCTGTT CTTTATCGTG 
CTCGTGAAAG TTAAAGACTC CACTGAGTAC ACAGGCCCCG AGAGCTACGT GGCTGAGATG 
ATAAAGGAGC ACAACCTGGA CTGGTTCCCA CGGATGCGTG CCATGTCTCT GGTGAGCAGC 
GACGCCGAGG GGGAGCAGAA TGAAATCAGG AACCTGCAGG AGAAGCTGGA GTCGACCATG 
AGGCTGGTGG CCAACCTGTC TGGACAGCTG AGTGAGCTCA AGGATCAGAT GACAGAGCAG 
AGGAAACACA AGCAACGGAT CGGTCTCCTT GGACACCCCC CCCACATGAA CATCAATCCA 
CAGCAACCAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074739 ( XM_011613734.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011612036.1 Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X2, mRNA.

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CloneID	OTo28517
Clone ID Related Accession (Same CDS sequence)	XM_011613734.1
Accession Version	XM_011613734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA AGATGTCCAG CTTCCTGCAC ATTGGGGACA TCTGCTCGCT GTATGCAGAG 
GGCTCCACCA ATGGTTTCAT CAGCACTCTC GGCTTGGTGG ACGACCGCTG CGTCGTCCAG 
CCCGACGCCG GCGACCTCAA CAACCCCCCG AAGAAGTTCC GAGACTGCCT GTTCAGACTG 
TGTCCCATGA ACCGCTACTC GGCCCAGAAG CAGTTCTGGA AAGCGGCGAA GCCTGGAGGG 
AACACCGACG CTGTGCTCCT CAACAAGCTG CATCATGCCG CCGACCTGGA GAAGAAGCAG 
AACGACTCTG AGAACAAAAA GCTGCTTGGA ACAGTGATTC AGTACGGGAA CGTCATTCAG 
CTCCTCCACC TGAAGAGCAA CAAGTACCTG ACGGTGAACA AGCGTCTACC CGCTCTGCTT 
GAGAAGAATG CCATGAGGGT GATGCTGGAC ACAGCAGGAA ATGAGGGCTC CTGGTTCTAC 
ATCCAGCCCT TCTACAAACT GCGCTCCATC GGAGACAGCG TGGTGATTGG AGACAAAGTG 
GTTCTGAACC CAGTGAACGC CGGTCAGCCG CTGCACGCCA GCACGCACCA GCTGGTGGAT 
AATCCAGGAT GCAACGAGGT GAACTCGGTC AACTGTAACA CTAGCTGGAA AATCGTCCTG 
TTTATGAAAT GGAGCGATAA CCAAGAGATC GTTTTGAAAG GGGGTGACGT TGTGCGGTTA 
TTTCACGCAG AGCAGGAGAA GTTTCTCACG TGTGACGATC ACAGGAAAAA ACAGTATGTC 
TTCCTGCGCA CTACCGGACG TCAGTCTGCA ACGTCGGCCA CCAGCAGCAA AGCACTGTGG 
GAAGTCGAGG TTGTGCAGCA CGATCCGTGT CGAGGGGGGG CTGGCTACTG GAACAGTCTG 
TTCAGGTTCA AGCACCTGGC CACTGGGCAC TACCTGGCTG CAGAGGTGAA TCCAGAGTAT 
GAAGAGGAGG GTCCGGAATC ACGATCCCCT CTGGAGTTGG AGCAGGAAGC CCTCCGAGCC 
CGGCTGAGGA ACGTTCAGGA CAAGGTCATG TACACCCTTG TGTCTGTCCC TGACGGGAAT 
GACATCTCTT CCATTTTTGA GCTCGACCCT ACCACGCTAA GAGGGGGGGA CTCCCTGGTG 
CCCAGGAACT CGTATGTGCG TCTCAGACAC CTCTGCACCA ACACATGGGT TCACAGCACC 
AATCAGCCAA TCGACAAAGA GGAGGAGAAG CCTGTCATGT TGCGTATTGG CACCTCTACT 
CTAAAAGAGG ACAAGGAGGC CTTTGCCATT GTGCCCGTCT CCCCTGCTGA GGTGAGAGAC 
CTCGACTTTG CTAATGATGC CAGTAAAGTG CTTGCTTCTA TTGCTGGGAA GCTGGAAAAA 
GGCACCATCA CGCAGAACGA GAGGAGAGCG GTCACGAAGC TTCTGGAGGA CTTGGTGTTT 
TTTGTGGTGG ATATTCCCAA CAGTGGCCAG GATGTTCTGG AGATCATGGT CAACAAACCC 
AACAGGGAGC GTCAGAAGCT CATGAGGGAA CAGAATATTC TCAAACAGAT CTTCAAGCTG 
CTGCAGGCGC CCTTCACAGA CAGTGGGGAC GGACCCATGC TGAGACTGGA GGAGCTGGCC 
GACCAGCGCC ATGCCCCCTT CAGACACATC TGCCGACTGT GTTACCGCGT TTTGCGTCAC 
TCTCAGCAGG ACTACCGTAA AAATCAGGAA TATATAGCCA AACAGTTCCG CTTCATGCAG 
AAGCAGATCG GCTATGACGT GCTGGCCGAG GACACAATCA CAGCTCTGCT CCACAACAAC 
CGAAAGCTGC TGGAGAAGCA CATCACGGCC GCTGAGATCG ACACCTTCGT CAGCCTGGTT 
CGCAAGAACC GCGAGCCTAG GTTCCTGGAC TACTTGTCAG ACTTGTGCGT GTCGATGAAC 
AAGTCCATCC CCGTGACCCA GGAGCTCATC TGCAATGCAG TGCTGGATCA GGCTAACGCA 
GACATCCTCA TAGAGACTAA GCTGGTGCTG TCGAGATTTG AGATCGAGGG AGCGCCGCTC 
GGAGAAAACT CACTGGAGTC GGAGGAGGAC GAGGAGGAGG TGTGGCTGTT CTGGAAGGAC 
AATAATAATA AAGAAATCCG CAGTAAAAGC ATTCGGGAGC TGGCCCAGGA TGCCAAAGAG 
GGTCAGAAGG AGGACCAGGA AGTGATCGGT TACTACAGAT ACCAGTTGAA CCTGTTTGCC 
AGGATGTGTT TGGACCGGCA GTACCTGGCA ATCAACAAGA TTTCCGGCCA GTTGGACGTG 
GACCTAATCT TGCGCTGCAT GTCGGACGAA GACCTGCCCT TTGACCTGCG AGCCTCGTTC 
TGCCGCATGA TGCTGCACAT GCATGTGGAC CGCGATCCAC AGGAACAAGT CACACCGGTC 
AAATATGCAC GGCTCTGGTC TGAGATCCCC TCTGAGATCG CCATCGACGA CTATGACAAC 
GATGGGACGT CCAAGGACGA GATTAAAGAG CGCTTCTGTC AGACCATGGA GTTTGTCGAG 
AACTACCTGA GAGACGTTGT CTGTCAGAGT TTCCCGTTCG CAGATAAAGA AAAGAACAAG 
CTCACGTTTG AGGTGGTGAA CCTGGCAAGG AACCTGATTT ACTTCGGCTT CTACAACTTC 
AGTGACCTGC TGAGACTCAC CAAGATCCTG CTGGCCATTC TAGACTGCGT CCACGTCGGC 
ACCATTTTCG GTCTCAACAA GCTGGACAAA GAAGATGAGA GTAAAGGCAG CAATGTGATG 
AAGTCCATTC ACGGTGTGGG AGAGCTGATG TCCCACGTCG TCCTGAGAGG AGGGGGCTTT 
CTCCCCATGA CTTCAAGCAG CAGCACCAGC AGAGACACAG TTAAAACCCA GACTGAACCC 
GAGAAACAGG ACATTTTGGT CATGGACACC AAGCTGAAGA TAATAGAGAT CCTGCAGTTC 
ATCTTGAACG TGCGGCTGGA CTACAGGATC TCCTGTTTGC TGTCGATATT TAAGAGGGAA 
TTCGACGAGA GCAACTCCCA GAGCGAGCTA ACTGTGGGCG GAGCCGTGGA AGGGCCGAAC 
AACATGCCAG GAGCTTTGGA TTTTGAACAC ATCGAAGAAC AGGCAGAAGG CATATTTGGT 
GGAAGTGAAG AAAACACACC ACTGGACCTG GATGATCATG GCGGCCGTAC CTTTTTGAGG 
GTCCTGCTCC ACTTAACCAT GCATGATTAT CCACCACTGG TATCTAGAGC GCTCCAGTTG 
CTCTTCAGGC ATTTCAGCCA AAGGCAGGAG GTTTTGCAGG CTTTCAAGCA GGTCCAGCTG 
CTCGTCACCA GCCAGGATGT GGAAAATTAC AAACAAATCA AGTCGGACCT TGACCAGCTT 
CGCTCCATCG TGGAGAAGTC CGAACTCTGG GTGTACAAGC GGCAGGGCTC CGAGGAGGGG 
ATGGATGCAG GAGAGCCTGA GCACAAACTG GGTGATGCAG TGGGCACAGA CAAGACAAAG 
AAACCCGAAA GCACCAGCAG TTACAATTAC AGGGTGGTGA AGGAGATCCT GCTGCGGCTC 
AGCAAGTTGT GTGTCCAAGA AGGTCTGTCA GGCAGGAAGA GCAAGAAGCA GCAGCAGAGG 
CTGCTGAGGA ACATGGGGGC CCACGCCGTT GTTCTCGAGC TGCTTCAGAT CCCATACGAG 
AAGGGAGAAG ATTTGCGGAT GCAGGACATC ATGAAATTAG CCCACCAGTT TCTACAGAAC 
TTTTGTGCTG GGAACCAGCA GAACCAAGCC CTGCTGCACA AGCATATCAA TCTCTTCCTC 
AACCCCGGGA TTTTGGAAGC CATCACCATG CAGCACATCT TCATGAACAA CTTCCAGCTG 
TGCAGCGAGA TAAACGAGCG CGTGGTTCAG CACTTTGTCC ACTGCATCGA GACGCACGGG 
CGCAACGTTC AGTATCTGAA ATTCCTTCAG ACTATCGTCA AAGCGGAGAA CAAGTTCATC 
AAAAAGTGTC AGGACATCGT CATGGCTGAG CTCGTAAACG CCGGCGAGGA CGTTTTGGTC 
TTCTACAACG ACCGCGCCTC CTTCCAGACG CTGGTGCAGA TGATGCGATC GGAGCGGGAC 
CGCATGGATG AAAACAGTCC CCTGATGTAC CACATCCACC TAGTAGAGCT ACTGGCCGTG 
TGCACGGAGG GCAAGAATGT CTACACAGAG ATCAAGTGCA ACTCCTTGCT ACCTCTAGAT 
GACATCGTGC GGGTGGTGAC GCATGAGGAC TGTATTCCCG AGGTGAAGAT CGCTTACATC 
AACTTCCTAA ACCACTGCTA TGTCGACACG GAAGTGGAGA TGAAAGAGAT CTACACCTCC 
AACCACATGT GGAAACTCTT TGAGAACTTT CTGGTCGACA TCTGCCGGGT GTGTAACAGC 
ACGAGCGAGC GCAAGCACGC CGACACCATT CTGGAGCGCT ACGTGACGGA GACGGTCATG 
AGCATCGTAA CGACGTTCTT CAGCTCGCCA TTCTCGGACC AGAGCACCAG CCTGCAGACC 
AGACAGCCTG TCTTTGTTCA GCTATTGCAG GCGATTTTCA GGGTGTACCA CTGTAACTGG 
CTGATCCCAG TTCAGAAGGG CTCTGTAGAG AGCTGCATTA AGGTGCTCTC TGATGTTGCC 
AAAGGTCGAG CAATAGCCAT CCCGGTGGAC CTGGACAACC AGGTGCACAA CCTTTTTGTG 
AAATCCAACA ATATTGTTCA GAAGACGGCC ATGAGCTGGA GACTTTCGGC CCGCAATGCT 
GCCCGCAGGG ACTCGATGGT GACTGCCTCA CGGGACTACA GGAACATCAT AGAGAGACTA 
CAGGACATCG TGTCAGCACT GGAGGACCGT CTGCGCCCGC TGGTCCAGGC CGAGTTATCG 
GTCCTGGTGG ACGTGCTGCA TCGGCCTGAG CTGCTCTTCC CTGAAAACAC CGACTCGCGC 
AAGAAGTGCG AGAGCGGAGG TTTTATCTGC AAGCTGATCA AACATACCAA GCAGCTGCTG 
GAGGAAAATG AAGAGAGGCT GTGCATCAAA GTCCTGCAAA CTCTGCGAGA GATGATGACA 
AAAGACCGAG GCTATGGAGA GAAGGGCGAG GCGCTCAGAC AGATTCTGGT GAACCGTTAC 
TATGGCAACT TCCACCGGAG CGGCGGACGC AGGGACAGCC TGACCTTTGC CAACGGCCCG 
CTCAGTCCGA TCGGGCCTGG CAAAAGCCAA TCAGGCTTGG GGGGTCCTGG GGCCTTGAGC 
CGTGGGGAGA TGAGCCTGGC GGAGGTTCAG TGTCACTTGG ACAAGGAGGG AGCCTCGGAC 
CTGGTGATCG ATCTCATCAT GAACACCACA AGCGACCGAG TCTTCCAAGA AAGCATCCTG 
CTGGCTATCG CACTGCTCGA AGGTGGAAAC ACAACCATCC AGCGGTCATT CTTTTGTCGC 
CTGACTGAAG ACAAGAAGTC AGAGAAGTTC TTCCGGGTCT TCTACGACCG TATGAAGCTG 
GCTCAGCTGG AGATCAAAGC CACTGTGACG GTAAACACCA GCGATTTAGG AAACAGGAAG 
CGAGAAGATG ACAACCAGGA CAAAGACGGC CCTGTTCGTA AAAAAGCCAG AGAATCTTCT 
GTGGTCATGA CGGAGGATGT GCGAGAACAG CTGTTAGAAG CATCTTCTGC CACCAAGAAA 
GCCTTCAACT CTTATCGGCG TGAAGCAGAC CCCGAGGACC ACTTCAACGT AGTGGACGGC 
CAGTCCAGCG CGGGAGACAA GAACCAGGAT GAAGGAGAAA TGAGCTTTGT TATCGTCATC 
ATGCAGCCGA TCCTTCGCTT CCTTCAGCTG CTGTGTGAGA ACCACAACCG GGATCTGCAG 
AACTTCCTCC GCTGTCAAAA TAACAAGAAC AACTACAACC TTGTGTGCGA GACCCTTCAG 
TTCTTGGACT GTATCTGTGG CAGCACCACG GGGGGCCTCG GCCTACTGGG CCTTTACATC 
AATGAGAAGA ATGTGGCCCT CATTAACCAA ACCCTGGAGA GTCTGACTGA GTACTGTCAG 
GGCCCCTGCC ATGAAAACCA GAATTGTATC GCCACCCATG AGTCCAATGG AATTGACATT 
ATCATCGCCC TGATTCTTAA CGACATCAAC CCACTTGGGA AGAAGCGAAT GGACCTGGTG 
CTGGAGCTGA AGAACAATGC GTCCAAGCTG CTGCTGGCAA TCATGGAGAG TCGACATGAC 
AGTGAGAATG CAGAGAGGAT CCTGTATAAT ATGAGACCCA AAGAGCTGGT GGAGGTGATA 
AAGAAGGCTT ACTTACAAGG AGAGGTGGAG ATCGAGGACA CAAAAGAGGA GGAAGAGAAT 
GAAGAGGAAG ATCACGACGC TGCTTCTCCC CGAAACGTTG GACACAACAT CTATATTTTA 
GCTCACCAGT TGGCGCGTCA CAATAAGGAG CTGGCGGTGA TGTTAAAGCC AGGCGTGGCC 
GTTGGGGAGG GAGATGAGGC CCTGGAGTGT TACGCCAAAC ACACCGCTCA GATCGAGATT 
GTGCGTCAGG ACCGTACCAT GGAGGAGATC GTGTTCCCAG TGCCAAACAT CTGCGAGTTC 
CTGACCACCG AATCCAAGCT GCGCGTCTAC TATACCACAG AGAGAGATGA GCAGGGCAGC 
AAGATCAACG ACTTCTTCTT GCGAGCGGAA GACCTCTTTA ATGAGATGAA CTGGCAGAAG 
AAGCTTAGAG CCCAGCCAGT GTTGTACTGG TGCTCCAGGA ACATGTCGGT CTGGAGTAAC 
ATTTGCTTTA ACCTGGCCTT ACTCATGAAC CTGCTGGTCT GCCTCTTCTA CCCGCTGGAA 
GGGGTGCAAG GAGTTATGCT GGATCCCCAC CTGTCTGCCC TGCTGTGGAT GGGGGTCGTT 
GCGGCCCTGG TGATTGTCCT CGTCATGCCT CAGCCGCTGA GCGTCCGCGC CCTCGTCATC 
ATCACCATCC TCCGCCTCAT CTTCTCGGTC GGCCTTGAGC CCACCCTCTT CCTTCTGGGC 
GCCTTGAATG TGTGCAATAA AATCATCTTC ATGATCAGCT TCGTGGGGAA CCGGGGAACC 
TTTACGCGCG GCTACAAAGC CATGGTGCTG GACTTTGAGT TCCTCTACCA CCTCATTTAC 
CTCATCATTT GCTGTCTAGG AGTGTTCGTC CACGTCTTCT TCTACAGCCT GCTGCTTTTT 
GACCTCGTGT ACCGAGAGGA GACGCTGCTG AACGTGATCA AGAGTGTGAC CCGCAACGGC 
CGCTCCATCG TGCTGACCGC CGTGCTGGCT CTCATCCTGG TCTACCTGTT CTCCATCGTC 
GGCTACATTT TCTTCAAGGA TGACTTCATC CTGGAGGTGG ACCGCGTCCC CAACGCAACT 
ACGAAAGGTG GAGGCAGTCT GGCCAGCGTG TTTGTTCCCG CTGGAATGTG TCAGGGAGGG 
ACTGATCAGA ACTGCACCGC AAGGGCCCAG CGGGAAGGTG ATCGGGACCG CGTCGGTGGT 
GACGGCGACG GGGACAAGGA CGAGGACGTG GAGCGGACGT GCGACTCCCT GCTGATGTGC 
ATCGTTACCG TCCTGAGTCA CGGCTTGAGG AGCGGAGGAG GAGTCGGTGA TGTTCTTCGC 
AAGCCTTCCA AGGAGGAGCC TCTGTTTGCC GCCAGAGTGA TATACGACTT GCTCTTCTTC 
TTCATGGTCA TCATCATCGT CCTCAACCTC ATCTTCGGTG TCATCATTGA CACCTTCGCT 
GACCTGAGGA GTGAGAAACA GAAGAAAGAG GAGGTTCTGA AAACGACCTG CTTCATCTGC 
GGTCTGGAGC GGGACAAGTT CGACAACAAG ACCGTGACGT TTGAGGAGCA CATCAAGGAG 
GAACACAACA TGTGGCACTA CCTGTTCTTT ATCGTGCTCG TGAAAGTTAA AGACTCCACT 
GAGTACACAG GCCCCGAGAG CTACGTGGCT GAGATGATAA AGGAGCACAA CCTGGACTGG 
TTCCCACGGA TGCGTGCCAT GTCTCTGGTG AGCAGCGACG CCGAGGGGGA GCAGAATGAA 
ATCAGGAACC TGCAGGAGAA GCTGGAGTCG ACCATGAGGC TGGTGGCCAA CCTGTCTGGA 
CAGCTGAGTG AGCTCAAGGA TCAGATGACA GAGCAGAGGA AACACAAGCA ACGGATCGGT 
CTCCTTGGAC ACCCCCCCCA CATGAACATC AATCCACAGC AACCAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011612036.1
CDS	246..8396
Translation	MSDKMSSFLHIGDICSLYAEGSTNGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGNTDAVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVMLDTAGNEGSWFYIQPFYKLRSIGDSVVIGDKVVLNPVNAGQPLHASTHQLVDNPGCNEVNSVNCNTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDDHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPEYEEEGPESRSPLELEQEALRARLRNVQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSTLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAGKLEKGTITQNERRAVTKLLEDLVFFVVDIPNSGQDVLEIMVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRHSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDQANADILIETKLVLSRFEIEGAPLGENSLESEEDEEEVWLFWKDNNNKEIRSKSIRELAQDAKEGQKEDQEVIGYYRYQLNLFARMCLDRQYLAINKISGQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSEIAIDDYDNDGTSKDEIKERFCQTMEFVENYLRDVVCQSFPFADKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHVGTIFGLNKLDKEDESKGSNVMKSIHGVGELMSHVVLRGGGFLPMTSSSSTSRDTVKTQTEPEKQDILVMDTKLKIIEILQFILNVRLDYRISCLLSIFKREFDESNSQSELTVGGAVEGPNNMPGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALQLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSEEGMDAGEPEHKLGDAVGTDKTKKPESTSSYNYRVVKEILLRLSKLCVQEGLSGRKSKKQQQRLLRNMGAHAVVLELLQIPYEKGEDLRMQDIMKLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAITMQHIFMNNFQLCSEINERVVQHFVHCIETHGRNVQYLKFLQTIVKAENKFIKKCQDIVMAELVNAGEDVLVFYNDRASFQTLVQMMRSERDRMDENSPLMYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKIAYINFLNHCYVDTEVEMKEIYTSNHMWKLFENFLVDICRVCNSTSERKHADTILERYVTETVMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQAIFRVYHCNWLIPVQKGSVESCIKVLSDVAKGRAIAIPVDLDNQVHNLFVKSNNIVQKTAMSWRLSARNAARRDSMVTASRDYRNIIERLQDIVSALEDRLRPLVQAELSVLVDVLHRPELLFPENTDSRKKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQILVNRYYGNFHRSGGRRDSLTFANGPLSPIGPGKSQSGLGGPGALSRGEMSLAEVQCHLDKEGASDLVIDLIMNTTSDRVFQESILLAIALLEGGNTTIQRSFFCRLTEDKKSEKFFRVFYDRMKLAQLEIKATVTVNTSDLGNRKREDDNQDKDGPVRKKARESSVVMTEDVREQLLEASSATKKAFNSYRREADPEDHFNVVDGQSSAGDKNQDEGEMSFVIVIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINEKNVALINQTLESLTEYCQGPCHENQNCIATHESNGIDIIIALILNDINPLGKKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVIKKAYLQGEVEIEDTKEEEENEEEDHDAASPRNVGHNIYILAHQLARHNKELAVMLKPGVAVGEGDEALECYAKHTAQIEIVRQDRTMEEIVFPVPNICEFLTTESKLRVYYTTERDEQGSKINDFFLRAEDLFNEMNWQKKLRAQPVLYWCSRNMSVWSNICFNLALLMNLLVCLFYPLEGVQGVMLDPHLSALLWMGVVAALVIVLVMPQPLSVRALVIITILRLIFSVGLEPTLFLLGALNVCNKIIFMISFVGNRGTFTRGYKAMVLDFEFLYHLIYLIICCLGVFVHVFFYSLLLFDLVYREETLLNVIKSVTRNGRSIVLTAVLALILVYLFSIVGYIFFKDDFILEVDRVPNATTKGGGSLASVFVPAGMCQGGTDQNCTARAQREGDRDRVGGDGDGDKDEDVERTCDSLLMCIVTVLSHGLRSGGGVGDVLRKPSKEEPLFAARVIYDLLFFFMVIIIVLNLIFGVIIDTFADLRSEKQKKEEVLKTTCFICGLERDKFDNKTVTFEEHIKEEHNMWHYLFFIVLVKVKDSTEYTGPESYVAEMIKEHNLDWFPRMRAMSLVSSDAEGEQNEIRNLQEKLESTMRLVANLSGQLSELKDQMTEQRKHKQRIGLLGHPPHMNINPQQPA

Target ORF information:

RefSeq Version	XM_011613734.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011613734.1

ORF Insert Sequence:

ATGTCGGACA AGATGTCCAG CTTCCTGCAC ATTGGGGACA TCTGCTCGCT GTATGCAGAG 
GGCTCCACCA ATGGTTTCAT CAGCACTCTC GGCTTGGTGG ACGACCGCTG CGTCGTCCAG 
CCCGACGCCG GCGACCTCAA CAACCCCCCG AAGAAGTTCC GAGACTGCCT GTTCAGACTG 
TGTCCCATGA ACCGCTACTC GGCCCAGAAG CAGTTCTGGA AAGCGGCGAA GCCTGGAGGG 
AACACCGACG CTGTGCTCCT CAACAAGCTG CATCATGCCG CCGACCTGGA GAAGAAGCAG 
AACGACTCTG AGAACAAAAA GCTGCTTGGA ACAGTGATTC AGTACGGGAA CGTCATTCAG 
CTCCTCCACC TGAAGAGCAA CAAGTACCTG ACGGTGAACA AGCGTCTACC CGCTCTGCTT 
GAGAAGAATG CCATGAGGGT GATGCTGGAC ACAGCAGGAA ATGAGGGCTC CTGGTTCTAC 
ATCCAGCCCT TCTACAAACT GCGCTCCATC GGAGACAGCG TGGTGATTGG AGACAAAGTG 
GTTCTGAACC CAGTGAACGC CGGTCAGCCG CTGCACGCCA GCACGCACCA GCTGGTGGAT 
AATCCAGGAT GCAACGAGGT GAACTCGGTC AACTGTAACA CTAGCTGGAA AATCGTCCTG 
TTTATGAAAT GGAGCGATAA CCAAGAGATC GTTTTGAAAG GGGGTGACGT TGTGCGGTTA 
TTTCACGCAG AGCAGGAGAA GTTTCTCACG TGTGACGATC ACAGGAAAAA ACAGTATGTC 
TTCCTGCGCA CTACCGGACG TCAGTCTGCA ACGTCGGCCA CCAGCAGCAA AGCACTGTGG 
GAAGTCGAGG TTGTGCAGCA CGATCCGTGT CGAGGGGGGG CTGGCTACTG GAACAGTCTG 
TTCAGGTTCA AGCACCTGGC CACTGGGCAC TACCTGGCTG CAGAGGTGAA TCCAGAGTAT 
GAAGAGGAGG GTCCGGAATC ACGATCCCCT CTGGAGTTGG AGCAGGAAGC CCTCCGAGCC 
CGGCTGAGGA ACGTTCAGGA CAAGGTCATG TACACCCTTG TGTCTGTCCC TGACGGGAAT 
GACATCTCTT CCATTTTTGA GCTCGACCCT ACCACGCTAA GAGGGGGGGA CTCCCTGGTG 
CCCAGGAACT CGTATGTGCG TCTCAGACAC CTCTGCACCA ACACATGGGT TCACAGCACC 
AATCAGCCAA TCGACAAAGA GGAGGAGAAG CCTGTCATGT TGCGTATTGG CACCTCTACT 
CTAAAAGAGG ACAAGGAGGC CTTTGCCATT GTGCCCGTCT CCCCTGCTGA GGTGAGAGAC 
CTCGACTTTG CTAATGATGC CAGTAAAGTG CTTGCTTCTA TTGCTGGGAA GCTGGAAAAA 
GGCACCATCA CGCAGAACGA GAGGAGAGCG GTCACGAAGC TTCTGGAGGA CTTGGTGTTT 
TTTGTGGTGG ATATTCCCAA CAGTGGCCAG GATGTTCTGG AGATCATGGT CAACAAACCC 
AACAGGGAGC GTCAGAAGCT CATGAGGGAA CAGAATATTC TCAAACAGAT CTTCAAGCTG 
CTGCAGGCGC CCTTCACAGA CAGTGGGGAC GGACCCATGC TGAGACTGGA GGAGCTGGCC 
GACCAGCGCC ATGCCCCCTT CAGACACATC TGCCGACTGT GTTACCGCGT TTTGCGTCAC 
TCTCAGCAGG ACTACCGTAA AAATCAGGAA TATATAGCCA AACAGTTCCG CTTCATGCAG 
AAGCAGATCG GCTATGACGT GCTGGCCGAG GACACAATCA CAGCTCTGCT CCACAACAAC 
CGAAAGCTGC TGGAGAAGCA CATCACGGCC GCTGAGATCG ACACCTTCGT CAGCCTGGTT 
CGCAAGAACC GCGAGCCTAG GTTCCTGGAC TACTTGTCAG ACTTGTGCGT GTCGATGAAC 
AAGTCCATCC CCGTGACCCA GGAGCTCATC TGCAATGCAG TGCTGGATCA GGCTAACGCA 
GACATCCTCA TAGAGACTAA GCTGGTGCTG TCGAGATTTG AGATCGAGGG AGCGCCGCTC 
GGAGAAAACT CACTGGAGTC GGAGGAGGAC GAGGAGGAGG TGTGGCTGTT CTGGAAGGAC 
AATAATAATA AAGAAATCCG CAGTAAAAGC ATTCGGGAGC TGGCCCAGGA TGCCAAAGAG 
GGTCAGAAGG AGGACCAGGA AGTGATCGGT TACTACAGAT ACCAGTTGAA CCTGTTTGCC 
AGGATGTGTT TGGACCGGCA GTACCTGGCA ATCAACAAGA TTTCCGGCCA GTTGGACGTG 
GACCTAATCT TGCGCTGCAT GTCGGACGAA GACCTGCCCT TTGACCTGCG AGCCTCGTTC 
TGCCGCATGA TGCTGCACAT GCATGTGGAC CGCGATCCAC AGGAACAAGT CACACCGGTC 
AAATATGCAC GGCTCTGGTC TGAGATCCCC TCTGAGATCG CCATCGACGA CTATGACAAC 
GATGGGACGT CCAAGGACGA GATTAAAGAG CGCTTCTGTC AGACCATGGA GTTTGTCGAG 
AACTACCTGA GAGACGTTGT CTGTCAGAGT TTCCCGTTCG CAGATAAAGA AAAGAACAAG 
CTCACGTTTG AGGTGGTGAA CCTGGCAAGG AACCTGATTT ACTTCGGCTT CTACAACTTC 
AGTGACCTGC TGAGACTCAC CAAGATCCTG CTGGCCATTC TAGACTGCGT CCACGTCGGC 
ACCATTTTCG GTCTCAACAA GCTGGACAAA GAAGATGAGA GTAAAGGCAG CAATGTGATG 
AAGTCCATTC ACGGTGTGGG AGAGCTGATG TCCCACGTCG TCCTGAGAGG AGGGGGCTTT 
CTCCCCATGA CTTCAAGCAG CAGCACCAGC AGAGACACAG TTAAAACCCA GACTGAACCC 
GAGAAACAGG ACATTTTGGT CATGGACACC AAGCTGAAGA TAATAGAGAT CCTGCAGTTC 
ATCTTGAACG TGCGGCTGGA CTACAGGATC TCCTGTTTGC TGTCGATATT TAAGAGGGAA 
TTCGACGAGA GCAACTCCCA GAGCGAGCTA ACTGTGGGCG GAGCCGTGGA AGGGCCGAAC 
AACATGCCAG GAGCTTTGGA TTTTGAACAC ATCGAAGAAC AGGCAGAAGG CATATTTGGT 
GGAAGTGAAG AAAACACACC ACTGGACCTG GATGATCATG GCGGCCGTAC CTTTTTGAGG 
GTCCTGCTCC ACTTAACCAT GCATGATTAT CCACCACTGG TATCTAGAGC GCTCCAGTTG 
CTCTTCAGGC ATTTCAGCCA AAGGCAGGAG GTTTTGCAGG CTTTCAAGCA GGTCCAGCTG 
CTCGTCACCA GCCAGGATGT GGAAAATTAC AAACAAATCA AGTCGGACCT TGACCAGCTT 
CGCTCCATCG TGGAGAAGTC CGAACTCTGG GTGTACAAGC GGCAGGGCTC CGAGGAGGGG 
ATGGATGCAG GAGAGCCTGA GCACAAACTG GGTGATGCAG TGGGCACAGA CAAGACAAAG 
AAACCCGAAA GCACCAGCAG TTACAATTAC AGGGTGGTGA AGGAGATCCT GCTGCGGCTC 
AGCAAGTTGT GTGTCCAAGA AGGTCTGTCA GGCAGGAAGA GCAAGAAGCA GCAGCAGAGG 
CTGCTGAGGA ACATGGGGGC CCACGCCGTT GTTCTCGAGC TGCTTCAGAT CCCATACGAG 
AAGGGAGAAG ATTTGCGGAT GCAGGACATC ATGAAATTAG CCCACCAGTT TCTACAGAAC 
TTTTGTGCTG GGAACCAGCA GAACCAAGCC CTGCTGCACA AGCATATCAA TCTCTTCCTC 
AACCCCGGGA TTTTGGAAGC CATCACCATG CAGCACATCT TCATGAACAA CTTCCAGCTG 
TGCAGCGAGA TAAACGAGCG CGTGGTTCAG CACTTTGTCC ACTGCATCGA GACGCACGGG 
CGCAACGTTC AGTATCTGAA ATTCCTTCAG ACTATCGTCA AAGCGGAGAA CAAGTTCATC 
AAAAAGTGTC AGGACATCGT CATGGCTGAG CTCGTAAACG CCGGCGAGGA CGTTTTGGTC 
TTCTACAACG ACCGCGCCTC CTTCCAGACG CTGGTGCAGA TGATGCGATC GGAGCGGGAC 
CGCATGGATG AAAACAGTCC CCTGATGTAC CACATCCACC TAGTAGAGCT ACTGGCCGTG 
TGCACGGAGG GCAAGAATGT CTACACAGAG ATCAAGTGCA ACTCCTTGCT ACCTCTAGAT 
GACATCGTGC GGGTGGTGAC GCATGAGGAC TGTATTCCCG AGGTGAAGAT CGCTTACATC 
AACTTCCTAA ACCACTGCTA TGTCGACACG GAAGTGGAGA TGAAAGAGAT CTACACCTCC 
AACCACATGT GGAAACTCTT TGAGAACTTT CTGGTCGACA TCTGCCGGGT GTGTAACAGC 
ACGAGCGAGC GCAAGCACGC CGACACCATT CTGGAGCGCT ACGTGACGGA GACGGTCATG 
AGCATCGTAA CGACGTTCTT CAGCTCGCCA TTCTCGGACC AGAGCACCAG CCTGCAGACC 
AGACAGCCTG TCTTTGTTCA GCTATTGCAG GCGATTTTCA GGGTGTACCA CTGTAACTGG 
CTGATCCCAG TTCAGAAGGG CTCTGTAGAG AGCTGCATTA AGGTGCTCTC TGATGTTGCC 
AAAGGTCGAG CAATAGCCAT CCCGGTGGAC CTGGACAACC AGGTGCACAA CCTTTTTGTG 
AAATCCAACA ATATTGTTCA GAAGACGGCC ATGAGCTGGA GACTTTCGGC CCGCAATGCT 
GCCCGCAGGG ACTCGATGGT GACTGCCTCA CGGGACTACA GGAACATCAT AGAGAGACTA 
CAGGACATCG TGTCAGCACT GGAGGACCGT CTGCGCCCGC TGGTCCAGGC CGAGTTATCG 
GTCCTGGTGG ACGTGCTGCA TCGGCCTGAG CTGCTCTTCC CTGAAAACAC CGACTCGCGC 
AAGAAGTGCG AGAGCGGAGG TTTTATCTGC AAGCTGATCA AACATACCAA GCAGCTGCTG 
GAGGAAAATG AAGAGAGGCT GTGCATCAAA GTCCTGCAAA CTCTGCGAGA GATGATGACA 
AAAGACCGAG GCTATGGAGA GAAGGGCGAG GCGCTCAGAC AGATTCTGGT GAACCGTTAC 
TATGGCAACT TCCACCGGAG CGGCGGACGC AGGGACAGCC TGACCTTTGC CAACGGCCCG 
CTCAGTCCGA TCGGGCCTGG CAAAAGCCAA TCAGGCTTGG GGGGTCCTGG GGCCTTGAGC 
CGTGGGGAGA TGAGCCTGGC GGAGGTTCAG TGTCACTTGG ACAAGGAGGG AGCCTCGGAC 
CTGGTGATCG ATCTCATCAT GAACACCACA AGCGACCGAG TCTTCCAAGA AAGCATCCTG 
CTGGCTATCG CACTGCTCGA AGGTGGAAAC ACAACCATCC AGCGGTCATT CTTTTGTCGC 
CTGACTGAAG ACAAGAAGTC AGAGAAGTTC TTCCGGGTCT TCTACGACCG TATGAAGCTG 
GCTCAGCTGG AGATCAAAGC CACTGTGACG GTAAACACCA GCGATTTAGG AAACAGGAAG 
CGAGAAGATG ACAACCAGGA CAAAGACGGC CCTGTTCGTA AAAAAGCCAG AGAATCTTCT 
GTGGTCATGA CGGAGGATGT GCGAGAACAG CTGTTAGAAG CATCTTCTGC CACCAAGAAA 
GCCTTCAACT CTTATCGGCG TGAAGCAGAC CCCGAGGACC ACTTCAACGT AGTGGACGGC 
CAGTCCAGCG CGGGAGACAA GAACCAGGAT GAAGGAGAAA TGAGCTTTGT TATCGTCATC 
ATGCAGCCGA TCCTTCGCTT CCTTCAGCTG CTGTGTGAGA ACCACAACCG GGATCTGCAG 
AACTTCCTCC GCTGTCAAAA TAACAAGAAC AACTACAACC TTGTGTGCGA GACCCTTCAG 
TTCTTGGACT GTATCTGTGG CAGCACCACG GGGGGCCTCG GCCTACTGGG CCTTTACATC 
AATGAGAAGA ATGTGGCCCT CATTAACCAA ACCCTGGAGA GTCTGACTGA GTACTGTCAG 
GGCCCCTGCC ATGAAAACCA GAATTGTATC GCCACCCATG AGTCCAATGG AATTGACATT 
ATCATCGCCC TGATTCTTAA CGACATCAAC CCACTTGGGA AGAAGCGAAT GGACCTGGTG 
CTGGAGCTGA AGAACAATGC GTCCAAGCTG CTGCTGGCAA TCATGGAGAG TCGACATGAC 
AGTGAGAATG CAGAGAGGAT CCTGTATAAT ATGAGACCCA AAGAGCTGGT GGAGGTGATA 
AAGAAGGCTT ACTTACAAGG AGAGGTGGAG ATCGAGGACA CAAAAGAGGA GGAAGAGAAT 
GAAGAGGAAG ATCACGACGC TGCTTCTCCC CGAAACGTTG GACACAACAT CTATATTTTA 
GCTCACCAGT TGGCGCGTCA CAATAAGGAG CTGGCGGTGA TGTTAAAGCC AGGCGTGGCC 
GTTGGGGAGG GAGATGAGGC CCTGGAGTGT TACGCCAAAC ACACCGCTCA GATCGAGATT 
GTGCGTCAGG ACCGTACCAT GGAGGAGATC GTGTTCCCAG TGCCAAACAT CTGCGAGTTC 
CTGACCACCG AATCCAAGCT GCGCGTCTAC TATACCACAG AGAGAGATGA GCAGGGCAGC 
AAGATCAACG ACTTCTTCTT GCGAGCGGAA GACCTCTTTA ATGAGATGAA CTGGCAGAAG 
AAGCTTAGAG CCCAGCCAGT GTTGTACTGG TGCTCCAGGA ACATGTCGGT CTGGAGTAAC 
ATTTGCTTTA ACCTGGCCTT ACTCATGAAC CTGCTGGTCT GCCTCTTCTA CCCGCTGGAA 
GGGGTGCAAG GAGTTATGCT GGATCCCCAC CTGTCTGCCC TGCTGTGGAT GGGGGTCGTT 
GCGGCCCTGG TGATTGTCCT CGTCATGCCT CAGCCGCTGA GCGTCCGCGC CCTCGTCATC 
ATCACCATCC TCCGCCTCAT CTTCTCGGTC GGCCTTGAGC CCACCCTCTT CCTTCTGGGC 
GCCTTGAATG TGTGCAATAA AATCATCTTC ATGATCAGCT TCGTGGGGAA CCGGGGAACC 
TTTACGCGCG GCTACAAAGC CATGGTGCTG GACTTTGAGT TCCTCTACCA CCTCATTTAC 
CTCATCATTT GCTGTCTAGG AGTGTTCGTC CACGTCTTCT TCTACAGCCT GCTGCTTTTT 
GACCTCGTGT ACCGAGAGGA GACGCTGCTG AACGTGATCA AGAGTGTGAC CCGCAACGGC 
CGCTCCATCG TGCTGACCGC CGTGCTGGCT CTCATCCTGG TCTACCTGTT CTCCATCGTC 
GGCTACATTT TCTTCAAGGA TGACTTCATC CTGGAGGTGG ACCGCGTCCC CAACGCAACT 
ACGAAAGGTG GAGGCAGTCT GGCCAGCGTG TTTGTTCCCG CTGGAATGTG TCAGGGAGGG 
ACTGATCAGA ACTGCACCGC AAGGGCCCAG CGGGAAGGTG ATCGGGACCG CGTCGGTGGT 
GACGGCGACG GGGACAAGGA CGAGGACGTG GAGCGGACGT GCGACTCCCT GCTGATGTGC 
ATCGTTACCG TCCTGAGTCA CGGCTTGAGG AGCGGAGGAG GAGTCGGTGA TGTTCTTCGC 
AAGCCTTCCA AGGAGGAGCC TCTGTTTGCC GCCAGAGTGA TATACGACTT GCTCTTCTTC 
TTCATGGTCA TCATCATCGT CCTCAACCTC ATCTTCGGTG TCATCATTGA CACCTTCGCT 
GACCTGAGGA GTGAGAAACA GAAGAAAGAG GAGGTTCTGA AAACGACCTG CTTCATCTGC 
GGTCTGGAGC GGGACAAGTT CGACAACAAG ACCGTGACGT TTGAGGAGCA CATCAAGGAG 
GAACACAACA TGTGGCACTA CCTGTTCTTT ATCGTGCTCG TGAAAGTTAA AGACTCCACT 
GAGTACACAG GCCCCGAGAG CTACGTGGCT GAGATGATAA AGGAGCACAA CCTGGACTGG 
TTCCCACGGA TGCGTGCCAT GTCTCTGGTG AGCAGCGACG CCGAGGGGGA GCAGAATGAA 
ATCAGGAACC TGCAGGAGAA GCTGGAGTCG ACCATGAGGC TGGTGGCCAA CCTGTCTGGA 
CAGCTGAGTG AGCTCAAGGA TCAGATGACA GAGCAGAGGA AACACAAGCA ACGGATCGGT 
CTCCTTGGAC ACCCCCCCCA CATGAACATC AATCCACAGC AACCAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074739 ( XM_011613733.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011612035.1 Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X1, mRNA.

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CloneID	OTo56667
Clone ID Related Accession (Same CDS sequence)	XM_011613733.2
Accession Version	XM_011613733.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042303.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011613733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA AGATGTCCAG CTTCCTGCAC ATTGGGGACA TCTGCTCGCT GTATGCAGAG 
GGCTCCACCA ATGGTTTCAT CAGCACTCTC GGCTTGGTGG ACGACCGCTG CGTCGTCCAG 
CCCGACGCCG GCGACCTCAA CAACCCCCCG AAGAAGTTCC GAGACTGCCT GTTCAGACTG 
TGTCCCATGA ACCGCTACTC GGCCCAGAAG CAGTTCTGGA AAGCGGCGAA GCCTGGAGGG 
AACACCGACG CTGTGCTCCT CAACAAGCTG CATCATGCCG CCGACCTGGA GAAGAAGCAG 
AATGACTCTG AGAACAAAAA GCTGCTTGGA ACAGTGATTC AGTACGGGAA CGTCATTCAG 
CTCCTCCACC TGAAGAGCAA CAAGTACCTG ACGGTGAACA AGCGTCTACC CGCTCTGCTT 
GAGAAGAATG CCATGAGGGT GATGCTGGAC ACAGCAGGAA ATGAGGGCTC CTGGTTCTAC 
ATCCAGCCCT TCTACAAACT GCGCTCCATC GGAGACAGCG TGGTGATTGG AGACAAAGTG 
GTTCTGAACC CAGTGAACGC CGGTCAGCCG CTGCACGCCA GCACGCACCA GCTGGTGGAT 
AATCCAGGAT GCAACGAGGT GAACTCGGTC AACTGTAACA CTAGCTGGAA AATCGTCCTG 
TTTATGAAAT GGAGCGATAA CCAAGAGATC GTTTTGAAAG GGGGTGACGT TGTGCGGTTA 
TTTCACGCAG AGCAGGAGAA GTTTCTCACG TGTGACGATC ACAGGAAAAA ACAGTATGTC 
TTCCTGCGCA CTACCGGACG TCAGTCTGCA ACGTCGGCCA CCAGCAGCAA AGCGCTGTGG 
GAAGTCGAGG TTGTGCAGCA CGATCCGTGT CGAGGGGGGG CTGGCTACTG GAACAGTCTG 
TTCAGGTTCA AGCACCTGGC CACTGGGCAC TACCTGGCTG CAGAGGTGAA TCCAGAGTAT 
GAAGAGGAGG GTCCGGAATC ACGATCCCCT CTGGAGTTGG AGCAGGAAGC CCTCCGAGCC 
CGGCTGAGGA ACGTTCAGGA CAAGGTCATG TACACCCTTG TGTCTGTCCC TGACGGGAAT 
GACATCTCTT CCATTTTTGA GCTCGACCCT ACCACGCTAA GAGGGGGGGA CTCCCTGGTG 
CCCAGGAACT CGTATGTGCG TCTCAGACAC CTCTGCACCA ACACATGGGT TCACAGCACC 
AATCAGCCAA TCGACAAAGA GGAGGAGAAG CCTGTCATGT TGCGTATTGG CACCTCTACT 
CTAAAAGAGG ACAAGGAGGC CTTTGCCATT GTGCCCGTCT CCCCTGCTGA GGTGAGAGAC 
CTCGACTTTG CTAATGATGC CAGTAAAGTG CTTGCTTCTA TTGCTGGGAA GCTGGAAAAA 
GGCACCATCA CGCAGAACGA GAGGAGAGCG GTCACGAAGC TTCTGGAGGA CTTGGTGTTT 
TTTGTGGTGG ATATTCCCAA CAGTGGCCAG GATGTTCTGG AGATCATGGT CAACAAACCC 
AACAGGGAGC GTCAGAAGCT CATGAGGGAA CAGAATATTC TCAAACAGAT CTTCAAGCTG 
CTGCAGGCGC CCTTCACAGA CAGTGGGGAC GGACCCATGC TGAGACTGGA GGAGCTGGCC 
GACCAGCGCC ATGCCCCCTT CAGACACATC TGCCGACTGT GTTACCGCGT TTTGCGTCAC 
TCTCAGCAGG ACTACCGTAA AAATCAGGAA TATATAGCCA AACAGTTCCG CTTCATGCAG 
AAGCAGATCG GCTATGACGT GCTGGCCGAG GACACAATCA CAGCTCTGCT CCACAACAAC 
CGAAAGCTGC TGGAGAAGCA CATCACGGCC GCTGAGATCG ACACCTTCGT CAGCCTGGTT 
CGCAAGAACC GCGAGCCTAG GTTCCTGGAC TACTTGTCAG ACTTGTGCGT GTCGATGAAC 
AAGTCCATCC CCGTGACCCA GGAGCTCATC TGCAATGCAG TGCTGGATCA GGCTAACGCA 
GACATCCTCA TAGAGACTAA GCTGGTGCTG TCGAGATTTG AGATCGAGGG AGCGCCGCTC 
GGAGAAAACT CACTGGAGTC GGAGGAGGAC GAGGAGGAGG TGTGGCTGTT CTGGAAGGAT 
AATAATAATA AAGAAATCCG CAGTAAAAGC ATTCGGGAGC TGGCCCAGGA TGCCAAAGAG 
GGTCAGAAGG AGGACCAGGA AGTGATCGGT TACTACAGAT ACCAGTTGAA CCTGTTTGCC 
AGGATGTGTT TGGACCGGCA GTACCTGGCA ATCAACAAGA TTTCCGGCCA GTTGGACGTG 
GACCTAATCT TGCGCTGCAT GTCGGACGAA GACCTGCCCT TTGACCTGCG AGCCTCGTTC 
TGCCGCATGA TGCTGCACAT GCATGTGGAC CGCGATCCAC AGGAACAAGT CACACCGGTC 
AAATATGCAC GGCTCTGGTC TGAGATCCCC TCTGAGATCG CCATCGACGA CTATGACAAC 
GATGGGACGT CCAAGGACGA GATTAAAGAG CGCTTCTGTC AGACCATGGA GTTTGTCGAG 
AACTACCTGA GAGACGTTGT CTGTCAGAGT TTCCCGTTCG CAGATAAAGA AAAGAACAAG 
CTCACGTTTG AGGTGGTGAA CCTGGCAAGG AACCTGATTT ACTTCGGCTT CTACAACTTC 
AGTGACCTGC TGAGACTCAC CAAGATCCTG CTGGCCATTC TAGACTGCGT CCACGTCGGC 
ACCATTTTCG GTCTCAACAA GCTGGACAAA GAAGATGAGA GTAAAGGCAG CAATGTGATG 
AAGTCCATTC ACGGTGTGGG AGAGCTGATG TCCCACGTCG TCCTGAGAGG AGGGGGCTTT 
CTCCCCATGA CTTCAAGCAG CAGCACCAGC AGAGACACAG TTAAAACCCA GACTGAACCC 
GAGAAACAGG ACATTTTGGT CATGGACACC AAGCTGAAGA TAATAGAGAT CCTGCAGTTC 
ATCTTGAACG TGCGGCTGGA CTACAGGATC TCCTGTTTGC TGTCGATATT TAAGAGGGAA 
TTCGACGAGA GCAACTCCCA GAGCGAGCTA ACTGTGGGCG GAGCCGTGGA AGGGCCGAAC 
AACATGCCAG GAGCTTTGGA TTTTGAACAC ATCGAAGAAC AGGCAGAAGG CATATTTGGT 
GGAAGTGAAG AAAACACACC ACTGGACCTG GATGATCATG GCGGCCGTAC CTTTTTGAGG 
GTCCTGCTCC ACTTAACCAT GCATGATTAT CCACCACTGG TATCTAGAGC GCTCCAGTTG 
CTCTTCAGGC ATTTCAGCCA AAGGCAGGAG GTTTTGCAGG CTTTCAAGCA GGTCCAGCTG 
CTCGTCACCA GCCAGGATGT GGAAAATTAC AAACAAATCA AGTCGGACCT TGACCAGCTT 
CGTTCCATCG TGGAGAAGTC CGAACTCTGG GTGTACAAGC GGCAGGGCTC CGAGGAGGGG 
ATGGATGCAG GAGAGCCTGA GCACAAACTG GGTGATGCAG TGGGCACAGA CAAGACAAAG 
AAACCCGAAA GCACCAGCAG TTACAATTAC AGGGTGGTGA AGGAGATCCT GCTGCGGCTC 
AGCAAGTTGT GTGTCCAAGA AGGTCTGTCA GGCAGGAAGA GCAAGAAGCA GCAGCAGAGG 
CTGCTGAGGA ACATGGGGGC CCACGCCGTT GTTCTCGAGC TGCTTCAGAT CCCATACGAG 
AAGGGAGAAG ATTTGCGGAT GCAGGACATC ATGAAATTAG CCCACCAGTT TCTACAGAAC 
TTTTGTGCTG GGAACCAGCA GAACCAAGCC CTGCTGCACA AGCATATCAA TCTCTTCCTC 
AACCCCGGGA TTTTGGAAGC CATCACCATG CAGCACATCT TCATGAACAA CTTCCAGCTG 
TGCAGCGAGA TAAACGAGCG CGTGGTTCAG CACTTTGTCC ACTGCATCGA GACGCACGGG 
CGCAACGTTC AGTATCTGAA ATTCCTTCAG ACTATCGTCA AAGCGGAGAA CAAGTTCATC 
AAAAAGTGTC AGGACATCGT CATGGCTGAG CTCGTAAACG CCGGCGAGGA CGTTTTGGTC 
TTCTACAACG ACCGCGCCTC CTTCCAGACA CTGGTGCAGA TGATGCGATC GGAGCGGGAC 
CGCATGGATG AAAACAGTCC CCTGATGTAC CACATCCACC TAGTAGAGCT ACTGGCCGTG 
TGCACGGAGG GCAAGAATGT CTACACAGAG ATCAAGTGCA ACTCCTTGCT ACCTCTAGAT 
GACATCGTGC GGGTGGTGAC GCATGAGGAC TGTATTCCCG AGGTGAAGAT CGCTTACATC 
AACTTCCTAA ACCACTGCTA TGTCGACACG GAAGTGGAGA TGAAAGAGAT CTACACCTCC 
AACCACATGT GGAAACTCTT TGAGAACTTT CTGGTCGACA TCTGCCGGGT GTGTAACAGC 
ACGAGCGAGC GCAAGCACGC CGACACCATT CTGGAGCGCT ACGTGACGGA GACGGTCATG 
AGCATCGTAA CGACGTTCTT CAGCTCGCCA TTCTCGGACC AGAGCACCAG CCTGCAGACC 
AGACAGCCTG TCTTTGTTCA GCTATTGCAG GCGATTTTCA GGGTGTACCA CTGTAACTGG 
CTGATCCCAG TTCAGAAGGG CTCTGTAGAG AGCTGCATTA AGGTGCTCTC TGATGTTGCC 
AAAGGTCGAG CAATAGCCAT CCCGGTGGAC CTGGACAACC AGGTGCACAA CCTTTTTGTG 
AAATCCAACA ATATTGTTCA GAAGACGGCC ATGAGCTGGA GACTTTCGGC CCGCAATGCT 
GCCCGCAGGG ACTCGATGGT GACTGCCTCA CGGGACTACA GGAACATCAT AGAGAGACTA 
CAGGACATCG TGTCAGCACT GGAGGACCGT CTGCGCCCGC TGGTCCAGGC CGAGTTATCG 
GTCCTGGTGG ACGTGCTGCA TCGGCCTGAG CTGCTCTTCC CTGAAAACAC CGACTCGCGC 
AAGAAGTGCG AGAGCGGAGG TTTTATCTGC AAGCTGATCA AACATACCAA GCAGCTGCTG 
GAGGAAAATG AAGAGAGGCT GTGCATCAAA GTCCTGCAAA CTCTGCGAGA GATGATGACA 
AAAGACCGAG GCTATGGAGA GAAGCTCAGG GCCTTTGATG ATGAAATGGA TATGCCCAAG 
CAGCTGGATC AGAACCAGCC CGAGAAACTG TTGGAGGACC AGAAGAGGGG CGAGGCGCTC 
AGACAGATTC TGGTGAACCG TTACTATGGC AACTTCCACC GGAGCGGCGG ACGCAGGGAC 
AGCCTGACCT TTGCCAACGG CCCGCTCAGT CCGATCGGGC CTGGCAAAAG CCAATCAGGC 
TTGGGGGGTC CTGGGGCCTT GAGCCGTGGG GAGATGAGCC TGGCGGAGGT TCAGTGTCAC 
TTGGACAAGG AGGGAGCCTC GGACCTGGTG ATCGATCTCA TCATGAACAC CACAAGCGAC 
CGAGTCTTCC AAGAAAGCAT CCTGCTGGCT ATCGCACTGC TCGAAGGTGG AAACACAACC 
ATCCAGCGGT CATTCTTTTG TCGCCTGACT GAAGACAAGA AGTCAGAGAA GTTCTTCCGG 
GTCTTCTACG ACCGTATGAA GCTGGCTCAG CTGGAGATCA AAGCCACTGT GACGGTAAAC 
ACCAGCGATT TAGGAAACAG GAAGCGAGAA GATGACAACC AGGACAAAGA CGGCCCTGTT 
CGTAAAAAAG CCAGAGAATC TTCTGTGGTC ATGACGGAGG ATGTGCGAGA ACAGCTGTTA 
GAAGCCTCTT CTGCCACCAA GAAAGCCTTC AACTCTTATC GGCGTGAAGC AGACCCCGAG 
GACCACTTCA ACGTAGTGGA CGGCCAGTCC AGCGCGGGAG ACAAGAACCA GGATGAAGGA 
GAAATGAGCT TTGTTATCGT CATCATGCAG CCGATCCTTC GCTTCCTTCA GCTGCTGTGT 
GAGAACCACA ACCGGGATCT GCAGAACTTC CTCCGCTGTC AAAATAACAA GAACAACTAC 
AACCTTGTGT GCGAGACCCT TCAGTTCTTG GACTGTATCT GTGGCAGCAC CACGGGGGGC 
CTCGGCCTAC TGGGCCTTTA CATCAATGAG AAGAATGTGG CCCTCATTAA CCAAACCCTG 
GAGAGTCTGA CTGAGTACTG TCAGGGCCCC TGCCATGAAA ACCAGAATTG TATCGCCACC 
CATGAGTCCA ATGGAATTGA CATTATCATC GCCCTGATTC TTAACGACAT CAACCCACTT 
GGGAAGAAGC GAATGGACCT GGTGCTGGAG CTGAAGAACA ATGCGTCCAA GCTGCTGCTG 
GCAATCATGG AGAGTCGACA TGACAGTGAG AATGCAGAGA GGATCCTGTA TAATATGAGA 
CCCAAAGAGC TGGTGGAGGT GATAAAGAAG GCTTACTTAC AAGGAGAGGT GGAGATCGAG 
GACACAAAAG AGGAGGAAGA GAATGAAGAG GAAGATCACG ACGCTGCTTC TCCCCGAAAC 
GTTGGACACA ACATCTATAT TTTAGCTCAC CAGTTGGCGC GTCACAATAA GGAGCTGGCG 
GTGATGTTAA AGCCAGGCGT GGCCGTTGGG GAGGGAGATG AGGCCCTGGA GTGTTACGCC 
AAACACACCG CTCAGATCGA GATTGTGCGT CAGGACCGTA CCATGGAGGA GATCGTGTTC 
CCAGTGCCAA ACATCTGCGA GTTCCTGACC ACCGAATCCA AGCTGCGCGT CTACTATACC 
ACAGAGAGAG ATGAGCAGGG CAGCAAGATC AATGACTTCT TCTTGCGAGC GGAAGACCTC 
TTTAATGAGA TGAACTGGCA GAAGAAGCTT AGAGCCCAGC CAGTGTTGTA CTGGTGCTCC 
AGGAACATGT CGGTCTGGAG TAACATTTGC TTTAACCTGG CCTTACTCAT GAACCTGCTG 
GTCTGCCTCT TCTACCCGCT GGAAGGGGTG CAAGGAGTTA TGCTGGATCC CCACCTGTCT 
GCCCTGCTGT GGATGGGGGT CGTTGCGGCC CTGGTCATTG TCCTCGTCAT GCCTCAGCCG 
CTGAGCGTCC GCGCCCTCGT CATCATCACC ATCCTCCGCC TCATCTTCTC GGTCGGCCTT 
GAGCCCACCC TCTTCCTTCT GGGCGCCTTG AATGTGTGCA ATAAAATCAT CTTCATGATC 
AGCTTCGTGG GGAACCGGGG AACCTTTACG CGCGGCTACA AAGCCATGGT GCTGGACTTT 
GAGTTCCTCT ACCACCTCAT TTACCTCATC ATTTGCTGTC TAGGAGTGTT CGTCCACGTC 
TTCTTCTACA GCCTGCTGCT TTTTGACCTC GTGTACCGAG AGGAGACGCT GCTGAACGTG 
ATCAAGAGTG TGACCCGCAA CGGCCGCTCC ATCGTGCTGA CCGCCGTGCT GGCTCTCATC 
CTGGTCTACC TGTTCTCCAT CGTCGGCTAC ATTTTCTTCA AGGATGACTT CATCCTGGAG 
GTGGACCGCG TCCCCAACGC AACTACGAAA GGTGGAGGCA GTCTGGCCAG CGTGTTTGTT 
CCCGCTGGAA TGTGTCAGGG AGGGACTGAT CAGAACTGCA CCGCAAGGGC CCAGCGGGAA 
GGTGATCGGG ACCGCGTCGG TGGTGACGGC GACGGGGACA AGGACGAGGA CGTGGAGCGG 
ACGTGCGACT CCCTGCTGAT GTGCATCGTT ACCGTCCTGA GTCACGGCTT GAGGAGCGGA 
GGAGGAGTCG GTGATGTTCT TCGCAAGCCT TCCAAGGAGG AGCCTCTGTT TGCCGCCAGA 
GTGATATACG ACTTGCTCTT CTTCTTCATG GTCATCATCA TCGTCCTCAA CCTCATCTTC 
GGTGTCATCA TTGACACCTT CGCTGACCTG AGGAGTGAGA AACAGAAGAA AGAGGAGGTT 
CTGAAAACGA CCTGCTTCAT CTGCGGTCTG GAGCGGGACA AGTTCGACAA CAAGACCGTG 
ACGTTTGAGG AGCACATCAA GGAGGAACAC AACATGTGGC ACTACCTGTT CTTTATCGTG 
CTCGTGAAAG TTAAAGACTC CACTGAGTAC ACAGGCCCCG AGAGCTACGT GGCTGAGATG 
ATAAAGGAGC ACAACCTGGA CTGGTTCCCA CGGATGCGTG CCATGTCTCT GGTGAGCAGC 
GACGCCGAGG GGGAGCAGAA TGAAATCAGG AACCTGCAGG AGAAGCTGGA GTCGACCATG 
AGGCTGGTGG CCAACCTGTC TGGACAGCTG AGTGAGCTCA AGGATCAGAT GACAGAGCAG 
AGGAAACACA AGCAACGGAT CGGTCTCCTT GGACACCCCC CCCACATGAA CATCAATCCA 
CAGCAACCAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011612035.1
CDS	255..8489
Translation	MSDKMSSFLHIGDICSLYAEGSTNGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGNTDAVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVMLDTAGNEGSWFYIQPFYKLRSIGDSVVIGDKVVLNPVNAGQPLHASTHQLVDNPGCNEVNSVNCNTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDDHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPEYEEEGPESRSPLELEQEALRARLRNVQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSTLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAGKLEKGTITQNERRAVTKLLEDLVFFVVDIPNSGQDVLEIMVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRHSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDQANADILIETKLVLSRFEIEGAPLGENSLESEEDEEEVWLFWKDNNNKEIRSKSIRELAQDAKEGQKEDQEVIGYYRYQLNLFARMCLDRQYLAINKISGQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSEIAIDDYDNDGTSKDEIKERFCQTMEFVENYLRDVVCQSFPFADKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHVGTIFGLNKLDKEDESKGSNVMKSIHGVGELMSHVVLRGGGFLPMTSSSSTSRDTVKTQTEPEKQDILVMDTKLKIIEILQFILNVRLDYRISCLLSIFKREFDESNSQSELTVGGAVEGPNNMPGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALQLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSEEGMDAGEPEHKLGDAVGTDKTKKPESTSSYNYRVVKEILLRLSKLCVQEGLSGRKSKKQQQRLLRNMGAHAVVLELLQIPYEKGEDLRMQDIMKLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAITMQHIFMNNFQLCSEINERVVQHFVHCIETHGRNVQYLKFLQTIVKAENKFIKKCQDIVMAELVNAGEDVLVFYNDRASFQTLVQMMRSERDRMDENSPLMYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKIAYINFLNHCYVDTEVEMKEIYTSNHMWKLFENFLVDICRVCNSTSERKHADTILERYVTETVMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQAIFRVYHCNWLIPVQKGSVESCIKVLSDVAKGRAIAIPVDLDNQVHNLFVKSNNIVQKTAMSWRLSARNAARRDSMVTASRDYRNIIERLQDIVSALEDRLRPLVQAELSVLVDVLHRPELLFPENTDSRKKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLRAFDDEMDMPKQLDQNQPEKLLEDQKRGEALRQILVNRYYGNFHRSGGRRDSLTFANGPLSPIGPGKSQSGLGGPGALSRGEMSLAEVQCHLDKEGASDLVIDLIMNTTSDRVFQESILLAIALLEGGNTTIQRSFFCRLTEDKKSEKFFRVFYDRMKLAQLEIKATVTVNTSDLGNRKREDDNQDKDGPVRKKARESSVVMTEDVREQLLEASSATKKAFNSYRREADPEDHFNVVDGQSSAGDKNQDEGEMSFVIVIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINEKNVALINQTLESLTEYCQGPCHENQNCIATHESNGIDIIIALILNDINPLGKKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVIKKAYLQGEVEIEDTKEEEENEEEDHDAASPRNVGHNIYILAHQLARHNKELAVMLKPGVAVGEGDEALECYAKHTAQIEIVRQDRTMEEIVFPVPNICEFLTTESKLRVYYTTERDEQGSKINDFFLRAEDLFNEMNWQKKLRAQPVLYWCSRNMSVWSNICFNLALLMNLLVCLFYPLEGVQGVMLDPHLSALLWMGVVAALVIVLVMPQPLSVRALVIITILRLIFSVGLEPTLFLLGALNVCNKIIFMISFVGNRGTFTRGYKAMVLDFEFLYHLIYLIICCLGVFVHVFFYSLLLFDLVYREETLLNVIKSVTRNGRSIVLTAVLALILVYLFSIVGYIFFKDDFILEVDRVPNATTKGGGSLASVFVPAGMCQGGTDQNCTARAQREGDRDRVGGDGDGDKDEDVERTCDSLLMCIVTVLSHGLRSGGGVGDVLRKPSKEEPLFAARVIYDLLFFFMVIIIVLNLIFGVIIDTFADLRSEKQKKEEVLKTTCFICGLERDKFDNKTVTFEEHIKEEHNMWHYLFFIVLVKVKDSTEYTGPESYVAEMIKEHNLDWFPRMRAMSLVSSDAEGEQNEIRNLQEKLESTMRLVANLSGQLSELKDQMTEQRKHKQRIGLLGHPPHMNINPQQPA

Target ORF information:

RefSeq Version	XM_011613733.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011613733.2

ORF Insert Sequence:

ATGTCGGACA AGATGTCCAG CTTCCTGCAC ATTGGGGACA TCTGCTCGCT GTATGCAGAG 
GGCTCCACCA ATGGTTTCAT CAGCACTCTC GGCTTGGTGG ACGACCGCTG CGTCGTCCAG 
CCCGACGCCG GCGACCTCAA CAACCCCCCG AAGAAGTTCC GAGACTGCCT GTTCAGACTG 
TGTCCCATGA ACCGCTACTC GGCCCAGAAG CAGTTCTGGA AAGCGGCGAA GCCTGGAGGG 
AACACCGACG CTGTGCTCCT CAACAAGCTG CATCATGCCG CCGACCTGGA GAAGAAGCAG 
AATGACTCTG AGAACAAAAA GCTGCTTGGA ACAGTGATTC AGTACGGGAA CGTCATTCAG 
CTCCTCCACC TGAAGAGCAA CAAGTACCTG ACGGTGAACA AGCGTCTACC CGCTCTGCTT 
GAGAAGAATG CCATGAGGGT GATGCTGGAC ACAGCAGGAA ATGAGGGCTC CTGGTTCTAC 
ATCCAGCCCT TCTACAAACT GCGCTCCATC GGAGACAGCG TGGTGATTGG AGACAAAGTG 
GTTCTGAACC CAGTGAACGC CGGTCAGCCG CTGCACGCCA GCACGCACCA GCTGGTGGAT 
AATCCAGGAT GCAACGAGGT GAACTCGGTC AACTGTAACA CTAGCTGGAA AATCGTCCTG 
TTTATGAAAT GGAGCGATAA CCAAGAGATC GTTTTGAAAG GGGGTGACGT TGTGCGGTTA 
TTTCACGCAG AGCAGGAGAA GTTTCTCACG TGTGACGATC ACAGGAAAAA ACAGTATGTC 
TTCCTGCGCA CTACCGGACG TCAGTCTGCA ACGTCGGCCA CCAGCAGCAA AGCGCTGTGG 
GAAGTCGAGG TTGTGCAGCA CGATCCGTGT CGAGGGGGGG CTGGCTACTG GAACAGTCTG 
TTCAGGTTCA AGCACCTGGC CACTGGGCAC TACCTGGCTG CAGAGGTGAA TCCAGAGTAT 
GAAGAGGAGG GTCCGGAATC ACGATCCCCT CTGGAGTTGG AGCAGGAAGC CCTCCGAGCC 
CGGCTGAGGA ACGTTCAGGA CAAGGTCATG TACACCCTTG TGTCTGTCCC TGACGGGAAT 
GACATCTCTT CCATTTTTGA GCTCGACCCT ACCACGCTAA GAGGGGGGGA CTCCCTGGTG 
CCCAGGAACT CGTATGTGCG TCTCAGACAC CTCTGCACCA ACACATGGGT TCACAGCACC 
AATCAGCCAA TCGACAAAGA GGAGGAGAAG CCTGTCATGT TGCGTATTGG CACCTCTACT 
CTAAAAGAGG ACAAGGAGGC CTTTGCCATT GTGCCCGTCT CCCCTGCTGA GGTGAGAGAC 
CTCGACTTTG CTAATGATGC CAGTAAAGTG CTTGCTTCTA TTGCTGGGAA GCTGGAAAAA 
GGCACCATCA CGCAGAACGA GAGGAGAGCG GTCACGAAGC TTCTGGAGGA CTTGGTGTTT 
TTTGTGGTGG ATATTCCCAA CAGTGGCCAG GATGTTCTGG AGATCATGGT CAACAAACCC 
AACAGGGAGC GTCAGAAGCT CATGAGGGAA CAGAATATTC TCAAACAGAT CTTCAAGCTG 
CTGCAGGCGC CCTTCACAGA CAGTGGGGAC GGACCCATGC TGAGACTGGA GGAGCTGGCC 
GACCAGCGCC ATGCCCCCTT CAGACACATC TGCCGACTGT GTTACCGCGT TTTGCGTCAC 
TCTCAGCAGG ACTACCGTAA AAATCAGGAA TATATAGCCA AACAGTTCCG CTTCATGCAG 
AAGCAGATCG GCTATGACGT GCTGGCCGAG GACACAATCA CAGCTCTGCT CCACAACAAC 
CGAAAGCTGC TGGAGAAGCA CATCACGGCC GCTGAGATCG ACACCTTCGT CAGCCTGGTT 
CGCAAGAACC GCGAGCCTAG GTTCCTGGAC TACTTGTCAG ACTTGTGCGT GTCGATGAAC 
AAGTCCATCC CCGTGACCCA GGAGCTCATC TGCAATGCAG TGCTGGATCA GGCTAACGCA 
GACATCCTCA TAGAGACTAA GCTGGTGCTG TCGAGATTTG AGATCGAGGG AGCGCCGCTC 
GGAGAAAACT CACTGGAGTC GGAGGAGGAC GAGGAGGAGG TGTGGCTGTT CTGGAAGGAT 
AATAATAATA AAGAAATCCG CAGTAAAAGC ATTCGGGAGC TGGCCCAGGA TGCCAAAGAG 
GGTCAGAAGG AGGACCAGGA AGTGATCGGT TACTACAGAT ACCAGTTGAA CCTGTTTGCC 
AGGATGTGTT TGGACCGGCA GTACCTGGCA ATCAACAAGA TTTCCGGCCA GTTGGACGTG 
GACCTAATCT TGCGCTGCAT GTCGGACGAA GACCTGCCCT TTGACCTGCG AGCCTCGTTC 
TGCCGCATGA TGCTGCACAT GCATGTGGAC CGCGATCCAC AGGAACAAGT CACACCGGTC 
AAATATGCAC GGCTCTGGTC TGAGATCCCC TCTGAGATCG CCATCGACGA CTATGACAAC 
GATGGGACGT CCAAGGACGA GATTAAAGAG CGCTTCTGTC AGACCATGGA GTTTGTCGAG 
AACTACCTGA GAGACGTTGT CTGTCAGAGT TTCCCGTTCG CAGATAAAGA AAAGAACAAG 
CTCACGTTTG AGGTGGTGAA CCTGGCAAGG AACCTGATTT ACTTCGGCTT CTACAACTTC 
AGTGACCTGC TGAGACTCAC CAAGATCCTG CTGGCCATTC TAGACTGCGT CCACGTCGGC 
ACCATTTTCG GTCTCAACAA GCTGGACAAA GAAGATGAGA GTAAAGGCAG CAATGTGATG 
AAGTCCATTC ACGGTGTGGG AGAGCTGATG TCCCACGTCG TCCTGAGAGG AGGGGGCTTT 
CTCCCCATGA CTTCAAGCAG CAGCACCAGC AGAGACACAG TTAAAACCCA GACTGAACCC 
GAGAAACAGG ACATTTTGGT CATGGACACC AAGCTGAAGA TAATAGAGAT CCTGCAGTTC 
ATCTTGAACG TGCGGCTGGA CTACAGGATC TCCTGTTTGC TGTCGATATT TAAGAGGGAA 
TTCGACGAGA GCAACTCCCA GAGCGAGCTA ACTGTGGGCG GAGCCGTGGA AGGGCCGAAC 
AACATGCCAG GAGCTTTGGA TTTTGAACAC ATCGAAGAAC AGGCAGAAGG CATATTTGGT 
GGAAGTGAAG AAAACACACC ACTGGACCTG GATGATCATG GCGGCCGTAC CTTTTTGAGG 
GTCCTGCTCC ACTTAACCAT GCATGATTAT CCACCACTGG TATCTAGAGC GCTCCAGTTG 
CTCTTCAGGC ATTTCAGCCA AAGGCAGGAG GTTTTGCAGG CTTTCAAGCA GGTCCAGCTG 
CTCGTCACCA GCCAGGATGT GGAAAATTAC AAACAAATCA AGTCGGACCT TGACCAGCTT 
CGTTCCATCG TGGAGAAGTC CGAACTCTGG GTGTACAAGC GGCAGGGCTC CGAGGAGGGG 
ATGGATGCAG GAGAGCCTGA GCACAAACTG GGTGATGCAG TGGGCACAGA CAAGACAAAG 
AAACCCGAAA GCACCAGCAG TTACAATTAC AGGGTGGTGA AGGAGATCCT GCTGCGGCTC 
AGCAAGTTGT GTGTCCAAGA AGGTCTGTCA GGCAGGAAGA GCAAGAAGCA GCAGCAGAGG 
CTGCTGAGGA ACATGGGGGC CCACGCCGTT GTTCTCGAGC TGCTTCAGAT CCCATACGAG 
AAGGGAGAAG ATTTGCGGAT GCAGGACATC ATGAAATTAG CCCACCAGTT TCTACAGAAC 
TTTTGTGCTG GGAACCAGCA GAACCAAGCC CTGCTGCACA AGCATATCAA TCTCTTCCTC 
AACCCCGGGA TTTTGGAAGC CATCACCATG CAGCACATCT TCATGAACAA CTTCCAGCTG 
TGCAGCGAGA TAAACGAGCG CGTGGTTCAG CACTTTGTCC ACTGCATCGA GACGCACGGG 
CGCAACGTTC AGTATCTGAA ATTCCTTCAG ACTATCGTCA AAGCGGAGAA CAAGTTCATC 
AAAAAGTGTC AGGACATCGT CATGGCTGAG CTCGTAAACG CCGGCGAGGA CGTTTTGGTC 
TTCTACAACG ACCGCGCCTC CTTCCAGACA CTGGTGCAGA TGATGCGATC GGAGCGGGAC 
CGCATGGATG AAAACAGTCC CCTGATGTAC CACATCCACC TAGTAGAGCT ACTGGCCGTG 
TGCACGGAGG GCAAGAATGT CTACACAGAG ATCAAGTGCA ACTCCTTGCT ACCTCTAGAT 
GACATCGTGC GGGTGGTGAC GCATGAGGAC TGTATTCCCG AGGTGAAGAT CGCTTACATC 
AACTTCCTAA ACCACTGCTA TGTCGACACG GAAGTGGAGA TGAAAGAGAT CTACACCTCC 
AACCACATGT GGAAACTCTT TGAGAACTTT CTGGTCGACA TCTGCCGGGT GTGTAACAGC 
ACGAGCGAGC GCAAGCACGC CGACACCATT CTGGAGCGCT ACGTGACGGA GACGGTCATG 
AGCATCGTAA CGACGTTCTT CAGCTCGCCA TTCTCGGACC AGAGCACCAG CCTGCAGACC 
AGACAGCCTG TCTTTGTTCA GCTATTGCAG GCGATTTTCA GGGTGTACCA CTGTAACTGG 
CTGATCCCAG TTCAGAAGGG CTCTGTAGAG AGCTGCATTA AGGTGCTCTC TGATGTTGCC 
AAAGGTCGAG CAATAGCCAT CCCGGTGGAC CTGGACAACC AGGTGCACAA CCTTTTTGTG 
AAATCCAACA ATATTGTTCA GAAGACGGCC ATGAGCTGGA GACTTTCGGC CCGCAATGCT 
GCCCGCAGGG ACTCGATGGT GACTGCCTCA CGGGACTACA GGAACATCAT AGAGAGACTA 
CAGGACATCG TGTCAGCACT GGAGGACCGT CTGCGCCCGC TGGTCCAGGC CGAGTTATCG 
GTCCTGGTGG ACGTGCTGCA TCGGCCTGAG CTGCTCTTCC CTGAAAACAC CGACTCGCGC 
AAGAAGTGCG AGAGCGGAGG TTTTATCTGC AAGCTGATCA AACATACCAA GCAGCTGCTG 
GAGGAAAATG AAGAGAGGCT GTGCATCAAA GTCCTGCAAA CTCTGCGAGA GATGATGACA 
AAAGACCGAG GCTATGGAGA GAAGCTCAGG GCCTTTGATG ATGAAATGGA TATGCCCAAG 
CAGCTGGATC AGAACCAGCC CGAGAAACTG TTGGAGGACC AGAAGAGGGG CGAGGCGCTC 
AGACAGATTC TGGTGAACCG TTACTATGGC AACTTCCACC GGAGCGGCGG ACGCAGGGAC 
AGCCTGACCT TTGCCAACGG CCCGCTCAGT CCGATCGGGC CTGGCAAAAG CCAATCAGGC 
TTGGGGGGTC CTGGGGCCTT GAGCCGTGGG GAGATGAGCC TGGCGGAGGT TCAGTGTCAC 
TTGGACAAGG AGGGAGCCTC GGACCTGGTG ATCGATCTCA TCATGAACAC CACAAGCGAC 
CGAGTCTTCC AAGAAAGCAT CCTGCTGGCT ATCGCACTGC TCGAAGGTGG AAACACAACC 
ATCCAGCGGT CATTCTTTTG TCGCCTGACT GAAGACAAGA AGTCAGAGAA GTTCTTCCGG 
GTCTTCTACG ACCGTATGAA GCTGGCTCAG CTGGAGATCA AAGCCACTGT GACGGTAAAC 
ACCAGCGATT TAGGAAACAG GAAGCGAGAA GATGACAACC AGGACAAAGA CGGCCCTGTT 
CGTAAAAAAG CCAGAGAATC TTCTGTGGTC ATGACGGAGG ATGTGCGAGA ACAGCTGTTA 
GAAGCCTCTT CTGCCACCAA GAAAGCCTTC AACTCTTATC GGCGTGAAGC AGACCCCGAG 
GACCACTTCA ACGTAGTGGA CGGCCAGTCC AGCGCGGGAG ACAAGAACCA GGATGAAGGA 
GAAATGAGCT TTGTTATCGT CATCATGCAG CCGATCCTTC GCTTCCTTCA GCTGCTGTGT 
GAGAACCACA ACCGGGATCT GCAGAACTTC CTCCGCTGTC AAAATAACAA GAACAACTAC 
AACCTTGTGT GCGAGACCCT TCAGTTCTTG GACTGTATCT GTGGCAGCAC CACGGGGGGC 
CTCGGCCTAC TGGGCCTTTA CATCAATGAG AAGAATGTGG CCCTCATTAA CCAAACCCTG 
GAGAGTCTGA CTGAGTACTG TCAGGGCCCC TGCCATGAAA ACCAGAATTG TATCGCCACC 
CATGAGTCCA ATGGAATTGA CATTATCATC GCCCTGATTC TTAACGACAT CAACCCACTT 
GGGAAGAAGC GAATGGACCT GGTGCTGGAG CTGAAGAACA ATGCGTCCAA GCTGCTGCTG 
GCAATCATGG AGAGTCGACA TGACAGTGAG AATGCAGAGA GGATCCTGTA TAATATGAGA 
CCCAAAGAGC TGGTGGAGGT GATAAAGAAG GCTTACTTAC AAGGAGAGGT GGAGATCGAG 
GACACAAAAG AGGAGGAAGA GAATGAAGAG GAAGATCACG ACGCTGCTTC TCCCCGAAAC 
GTTGGACACA ACATCTATAT TTTAGCTCAC CAGTTGGCGC GTCACAATAA GGAGCTGGCG 
GTGATGTTAA AGCCAGGCGT GGCCGTTGGG GAGGGAGATG AGGCCCTGGA GTGTTACGCC 
AAACACACCG CTCAGATCGA GATTGTGCGT CAGGACCGTA CCATGGAGGA GATCGTGTTC 
CCAGTGCCAA ACATCTGCGA GTTCCTGACC ACCGAATCCA AGCTGCGCGT CTACTATACC 
ACAGAGAGAG ATGAGCAGGG CAGCAAGATC AATGACTTCT TCTTGCGAGC GGAAGACCTC 
TTTAATGAGA TGAACTGGCA GAAGAAGCTT AGAGCCCAGC CAGTGTTGTA CTGGTGCTCC 
AGGAACATGT CGGTCTGGAG TAACATTTGC TTTAACCTGG CCTTACTCAT GAACCTGCTG 
GTCTGCCTCT TCTACCCGCT GGAAGGGGTG CAAGGAGTTA TGCTGGATCC CCACCTGTCT 
GCCCTGCTGT GGATGGGGGT CGTTGCGGCC CTGGTCATTG TCCTCGTCAT GCCTCAGCCG 
CTGAGCGTCC GCGCCCTCGT CATCATCACC ATCCTCCGCC TCATCTTCTC GGTCGGCCTT 
GAGCCCACCC TCTTCCTTCT GGGCGCCTTG AATGTGTGCA ATAAAATCAT CTTCATGATC 
AGCTTCGTGG GGAACCGGGG AACCTTTACG CGCGGCTACA AAGCCATGGT GCTGGACTTT 
GAGTTCCTCT ACCACCTCAT TTACCTCATC ATTTGCTGTC TAGGAGTGTT CGTCCACGTC 
TTCTTCTACA GCCTGCTGCT TTTTGACCTC GTGTACCGAG AGGAGACGCT GCTGAACGTG 
ATCAAGAGTG TGACCCGCAA CGGCCGCTCC ATCGTGCTGA CCGCCGTGCT GGCTCTCATC 
CTGGTCTACC TGTTCTCCAT CGTCGGCTAC ATTTTCTTCA AGGATGACTT CATCCTGGAG 
GTGGACCGCG TCCCCAACGC AACTACGAAA GGTGGAGGCA GTCTGGCCAG CGTGTTTGTT 
CCCGCTGGAA TGTGTCAGGG AGGGACTGAT CAGAACTGCA CCGCAAGGGC CCAGCGGGAA 
GGTGATCGGG ACCGCGTCGG TGGTGACGGC GACGGGGACA AGGACGAGGA CGTGGAGCGG 
ACGTGCGACT CCCTGCTGAT GTGCATCGTT ACCGTCCTGA GTCACGGCTT GAGGAGCGGA 
GGAGGAGTCG GTGATGTTCT TCGCAAGCCT TCCAAGGAGG AGCCTCTGTT TGCCGCCAGA 
GTGATATACG ACTTGCTCTT CTTCTTCATG GTCATCATCA TCGTCCTCAA CCTCATCTTC 
GGTGTCATCA TTGACACCTT CGCTGACCTG AGGAGTGAGA AACAGAAGAA AGAGGAGGTT 
CTGAAAACGA CCTGCTTCAT CTGCGGTCTG GAGCGGGACA AGTTCGACAA CAAGACCGTG 
ACGTTTGAGG AGCACATCAA GGAGGAACAC AACATGTGGC ACTACCTGTT CTTTATCGTG 
CTCGTGAAAG TTAAAGACTC CACTGAGTAC ACAGGCCCCG AGAGCTACGT GGCTGAGATG 
ATAAAGGAGC ACAACCTGGA CTGGTTCCCA CGGATGCGTG CCATGTCTCT GGTGAGCAGC 
GACGCCGAGG GGGAGCAGAA TGAAATCAGG AACCTGCAGG AGAAGCTGGA GTCGACCATG 
AGGCTGGTGG CCAACCTGTC TGGACAGCTG AGTGAGCTCA AGGATCAGAT GACAGAGCAG 
AGGAAACACA AGCAACGGAT CGGTCTCCTT GGACACCCCC CCCACATGAA CATCAATCCA 
CAGCAACCAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101074739 ( XM_011613734.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011612036.1 Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X2, mRNA.

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CloneID	OTo56668
Clone ID Related Accession (Same CDS sequence)	XM_011613734.2
Accession Version	XM_011613734.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042303.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011613734.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA AGATGTCCAG CTTCCTGCAC ATTGGGGACA TCTGCTCGCT GTATGCAGAG 
GGCTCCACCA ATGGTTTCAT CAGCACTCTC GGCTTGGTGG ACGACCGCTG CGTCGTCCAG 
CCCGACGCCG GCGACCTCAA CAACCCCCCG AAGAAGTTCC GAGACTGCCT GTTCAGACTG 
TGTCCCATGA ACCGCTACTC GGCCCAGAAG CAGTTCTGGA AAGCGGCGAA GCCTGGAGGG 
AACACCGACG CTGTGCTCCT CAACAAGCTG CATCATGCCG CCGACCTGGA GAAGAAGCAG 
AATGACTCTG AGAACAAAAA GCTGCTTGGA ACAGTGATTC AGTACGGGAA CGTCATTCAG 
CTCCTCCACC TGAAGAGCAA CAAGTACCTG ACGGTGAACA AGCGTCTACC CGCTCTGCTT 
GAGAAGAATG CCATGAGGGT GATGCTGGAC ACAGCAGGAA ATGAGGGCTC CTGGTTCTAC 
ATCCAGCCCT TCTACAAACT GCGCTCCATC GGAGACAGCG TGGTGATTGG AGACAAAGTG 
GTTCTGAACC CAGTGAACGC CGGTCAGCCG CTGCACGCCA GCACGCACCA GCTGGTGGAT 
AATCCAGGAT GCAACGAGGT GAACTCGGTC AACTGTAACA CTAGCTGGAA AATCGTCCTG 
TTTATGAAAT GGAGCGATAA CCAAGAGATC GTTTTGAAAG GGGGTGACGT TGTGCGGTTA 
TTTCACGCAG AGCAGGAGAA GTTTCTCACG TGTGACGATC ACAGGAAAAA ACAGTATGTC 
TTCCTGCGCA CTACCGGACG TCAGTCTGCA ACGTCGGCCA CCAGCAGCAA AGCGCTGTGG 
GAAGTCGAGG TTGTGCAGCA CGATCCGTGT CGAGGGGGGG CTGGCTACTG GAACAGTCTG 
TTCAGGTTCA AGCACCTGGC CACTGGGCAC TACCTGGCTG CAGAGGTGAA TCCAGAGTAT 
GAAGAGGAGG GTCCGGAATC ACGATCCCCT CTGGAGTTGG AGCAGGAAGC CCTCCGAGCC 
CGGCTGAGGA ACGTTCAGGA CAAGGTCATG TACACCCTTG TGTCTGTCCC TGACGGGAAT 
GACATCTCTT CCATTTTTGA GCTCGACCCT ACCACGCTAA GAGGGGGGGA CTCCCTGGTG 
CCCAGGAACT CGTATGTGCG TCTCAGACAC CTCTGCACCA ACACATGGGT TCACAGCACC 
AATCAGCCAA TCGACAAAGA GGAGGAGAAG CCTGTCATGT TGCGTATTGG CACCTCTACT 
CTAAAAGAGG ACAAGGAGGC CTTTGCCATT GTGCCCGTCT CCCCTGCTGA GGTGAGAGAC 
CTCGACTTTG CTAATGATGC CAGTAAAGTG CTTGCTTCTA TTGCTGGGAA GCTGGAAAAA 
GGCACCATCA CGCAGAACGA GAGGAGAGCG GTCACGAAGC TTCTGGAGGA CTTGGTGTTT 
TTTGTGGTGG ATATTCCCAA CAGTGGCCAG GATGTTCTGG AGATCATGGT CAACAAACCC 
AACAGGGAGC GTCAGAAGCT CATGAGGGAA CAGAATATTC TCAAACAGAT CTTCAAGCTG 
CTGCAGGCGC CCTTCACAGA CAGTGGGGAC GGACCCATGC TGAGACTGGA GGAGCTGGCC 
GACCAGCGCC ATGCCCCCTT CAGACACATC TGCCGACTGT GTTACCGCGT TTTGCGTCAC 
TCTCAGCAGG ACTACCGTAA AAATCAGGAA TATATAGCCA AACAGTTCCG CTTCATGCAG 
AAGCAGATCG GCTATGACGT GCTGGCCGAG GACACAATCA CAGCTCTGCT CCACAACAAC 
CGAAAGCTGC TGGAGAAGCA CATCACGGCC GCTGAGATCG ACACCTTCGT CAGCCTGGTT 
CGCAAGAACC GCGAGCCTAG GTTCCTGGAC TACTTGTCAG ACTTGTGCGT GTCGATGAAC 
AAGTCCATCC CCGTGACCCA GGAGCTCATC TGCAATGCAG TGCTGGATCA GGCTAACGCA 
GACATCCTCA TAGAGACTAA GCTGGTGCTG TCGAGATTTG AGATCGAGGG AGCGCCGCTC 
GGAGAAAACT CACTGGAGTC GGAGGAGGAC GAGGAGGAGG TGTGGCTGTT CTGGAAGGAT 
AATAATAATA AAGAAATCCG CAGTAAAAGC ATTCGGGAGC TGGCCCAGGA TGCCAAAGAG 
GGTCAGAAGG AGGACCAGGA AGTGATCGGT TACTACAGAT ACCAGTTGAA CCTGTTTGCC 
AGGATGTGTT TGGACCGGCA GTACCTGGCA ATCAACAAGA TTTCCGGCCA GTTGGACGTG 
GACCTAATCT TGCGCTGCAT GTCGGACGAA GACCTGCCCT TTGACCTGCG AGCCTCGTTC 
TGCCGCATGA TGCTGCACAT GCATGTGGAC CGCGATCCAC AGGAACAAGT CACACCGGTC 
AAATATGCAC GGCTCTGGTC TGAGATCCCC TCTGAGATCG CCATCGACGA CTATGACAAC 
GATGGGACGT CCAAGGACGA GATTAAAGAG CGCTTCTGTC AGACCATGGA GTTTGTCGAG 
AACTACCTGA GAGACGTTGT CTGTCAGAGT TTCCCGTTCG CAGATAAAGA AAAGAACAAG 
CTCACGTTTG AGGTGGTGAA CCTGGCAAGG AACCTGATTT ACTTCGGCTT CTACAACTTC 
AGTGACCTGC TGAGACTCAC CAAGATCCTG CTGGCCATTC TAGACTGCGT CCACGTCGGC 
ACCATTTTCG GTCTCAACAA GCTGGACAAA GAAGATGAGA GTAAAGGCAG CAATGTGATG 
AAGTCCATTC ACGGTGTGGG AGAGCTGATG TCCCACGTCG TCCTGAGAGG AGGGGGCTTT 
CTCCCCATGA CTTCAAGCAG CAGCACCAGC AGAGACACAG TTAAAACCCA GACTGAACCC 
GAGAAACAGG ACATTTTGGT CATGGACACC AAGCTGAAGA TAATAGAGAT CCTGCAGTTC 
ATCTTGAACG TGCGGCTGGA CTACAGGATC TCCTGTTTGC TGTCGATATT TAAGAGGGAA 
TTCGACGAGA GCAACTCCCA GAGCGAGCTA ACTGTGGGCG GAGCCGTGGA AGGGCCGAAC 
AACATGCCAG GAGCTTTGGA TTTTGAACAC ATCGAAGAAC AGGCAGAAGG CATATTTGGT 
GGAAGTGAAG AAAACACACC ACTGGACCTG GATGATCATG GCGGCCGTAC CTTTTTGAGG 
GTCCTGCTCC ACTTAACCAT GCATGATTAT CCACCACTGG TATCTAGAGC GCTCCAGTTG 
CTCTTCAGGC ATTTCAGCCA AAGGCAGGAG GTTTTGCAGG CTTTCAAGCA GGTCCAGCTG 
CTCGTCACCA GCCAGGATGT GGAAAATTAC AAACAAATCA AGTCGGACCT TGACCAGCTT 
CGTTCCATCG TGGAGAAGTC CGAACTCTGG GTGTACAAGC GGCAGGGCTC CGAGGAGGGG 
ATGGATGCAG GAGAGCCTGA GCACAAACTG GGTGATGCAG TGGGCACAGA CAAGACAAAG 
AAACCCGAAA GCACCAGCAG TTACAATTAC AGGGTGGTGA AGGAGATCCT GCTGCGGCTC 
AGCAAGTTGT GTGTCCAAGA AGGTCTGTCA GGCAGGAAGA GCAAGAAGCA GCAGCAGAGG 
CTGCTGAGGA ACATGGGGGC CCACGCCGTT GTTCTCGAGC TGCTTCAGAT CCCATACGAG 
AAGGGAGAAG ATTTGCGGAT GCAGGACATC ATGAAATTAG CCCACCAGTT TCTACAGAAC 
TTTTGTGCTG GGAACCAGCA GAACCAAGCC CTGCTGCACA AGCATATCAA TCTCTTCCTC 
AACCCCGGGA TTTTGGAAGC CATCACCATG CAGCACATCT TCATGAACAA CTTCCAGCTG 
TGCAGCGAGA TAAACGAGCG CGTGGTTCAG CACTTTGTCC ACTGCATCGA GACGCACGGG 
CGCAACGTTC AGTATCTGAA ATTCCTTCAG ACTATCGTCA AAGCGGAGAA CAAGTTCATC 
AAAAAGTGTC AGGACATCGT CATGGCTGAG CTCGTAAACG CCGGCGAGGA CGTTTTGGTC 
TTCTACAACG ACCGCGCCTC CTTCCAGACA CTGGTGCAGA TGATGCGATC GGAGCGGGAC 
CGCATGGATG AAAACAGTCC CCTGATGTAC CACATCCACC TAGTAGAGCT ACTGGCCGTG 
TGCACGGAGG GCAAGAATGT CTACACAGAG ATCAAGTGCA ACTCCTTGCT ACCTCTAGAT 
GACATCGTGC GGGTGGTGAC GCATGAGGAC TGTATTCCCG AGGTGAAGAT CGCTTACATC 
AACTTCCTAA ACCACTGCTA TGTCGACACG GAAGTGGAGA TGAAAGAGAT CTACACCTCC 
AACCACATGT GGAAACTCTT TGAGAACTTT CTGGTCGACA TCTGCCGGGT GTGTAACAGC 
ACGAGCGAGC GCAAGCACGC CGACACCATT CTGGAGCGCT ACGTGACGGA GACGGTCATG 
AGCATCGTAA CGACGTTCTT CAGCTCGCCA TTCTCGGACC AGAGCACCAG CCTGCAGACC 
AGACAGCCTG TCTTTGTTCA GCTATTGCAG GCGATTTTCA GGGTGTACCA CTGTAACTGG 
CTGATCCCAG TTCAGAAGGG CTCTGTAGAG AGCTGCATTA AGGTGCTCTC TGATGTTGCC 
AAAGGTCGAG CAATAGCCAT CCCGGTGGAC CTGGACAACC AGGTGCACAA CCTTTTTGTG 
AAATCCAACA ATATTGTTCA GAAGACGGCC ATGAGCTGGA GACTTTCGGC CCGCAATGCT 
GCCCGCAGGG ACTCGATGGT GACTGCCTCA CGGGACTACA GGAACATCAT AGAGAGACTA 
CAGGACATCG TGTCAGCACT GGAGGACCGT CTGCGCCCGC TGGTCCAGGC CGAGTTATCG 
GTCCTGGTGG ACGTGCTGCA TCGGCCTGAG CTGCTCTTCC CTGAAAACAC CGACTCGCGC 
AAGAAGTGCG AGAGCGGAGG TTTTATCTGC AAGCTGATCA AACATACCAA GCAGCTGCTG 
GAGGAAAATG AAGAGAGGCT GTGCATCAAA GTCCTGCAAA CTCTGCGAGA GATGATGACA 
AAAGACCGAG GCTATGGAGA GAAGGGCGAG GCGCTCAGAC AGATTCTGGT GAACCGTTAC 
TATGGCAACT TCCACCGGAG CGGCGGACGC AGGGACAGCC TGACCTTTGC CAACGGCCCG 
CTCAGTCCGA TCGGGCCTGG CAAAAGCCAA TCAGGCTTGG GGGGTCCTGG GGCCTTGAGC 
CGTGGGGAGA TGAGCCTGGC GGAGGTTCAG TGTCACTTGG ACAAGGAGGG AGCCTCGGAC 
CTGGTGATCG ATCTCATCAT GAACACCACA AGCGACCGAG TCTTCCAAGA AAGCATCCTG 
CTGGCTATCG CACTGCTCGA AGGTGGAAAC ACAACCATCC AGCGGTCATT CTTTTGTCGC 
CTGACTGAAG ACAAGAAGTC AGAGAAGTTC TTCCGGGTCT TCTACGACCG TATGAAGCTG 
GCTCAGCTGG AGATCAAAGC CACTGTGACG GTAAACACCA GCGATTTAGG AAACAGGAAG 
CGAGAAGATG ACAACCAGGA CAAAGACGGC CCTGTTCGTA AAAAAGCCAG AGAATCTTCT 
GTGGTCATGA CGGAGGATGT GCGAGAACAG CTGTTAGAAG CCTCTTCTGC CACCAAGAAA 
GCCTTCAACT CTTATCGGCG TGAAGCAGAC CCCGAGGACC ACTTCAACGT AGTGGACGGC 
CAGTCCAGCG CGGGAGACAA GAACCAGGAT GAAGGAGAAA TGAGCTTTGT TATCGTCATC 
ATGCAGCCGA TCCTTCGCTT CCTTCAGCTG CTGTGTGAGA ACCACAACCG GGATCTGCAG 
AACTTCCTCC GCTGTCAAAA TAACAAGAAC AACTACAACC TTGTGTGCGA GACCCTTCAG 
TTCTTGGACT GTATCTGTGG CAGCACCACG GGGGGCCTCG GCCTACTGGG CCTTTACATC 
AATGAGAAGA ATGTGGCCCT CATTAACCAA ACCCTGGAGA GTCTGACTGA GTACTGTCAG 
GGCCCCTGCC ATGAAAACCA GAATTGTATC GCCACCCATG AGTCCAATGG AATTGACATT 
ATCATCGCCC TGATTCTTAA CGACATCAAC CCACTTGGGA AGAAGCGAAT GGACCTGGTG 
CTGGAGCTGA AGAACAATGC GTCCAAGCTG CTGCTGGCAA TCATGGAGAG TCGACATGAC 
AGTGAGAATG CAGAGAGGAT CCTGTATAAT ATGAGACCCA AAGAGCTGGT GGAGGTGATA 
AAGAAGGCTT ACTTACAAGG AGAGGTGGAG ATCGAGGACA CAAAAGAGGA GGAAGAGAAT 
GAAGAGGAAG ATCACGACGC TGCTTCTCCC CGAAACGTTG GACACAACAT CTATATTTTA 
GCTCACCAGT TGGCGCGTCA CAATAAGGAG CTGGCGGTGA TGTTAAAGCC AGGCGTGGCC 
GTTGGGGAGG GAGATGAGGC CCTGGAGTGT TACGCCAAAC ACACCGCTCA GATCGAGATT 
GTGCGTCAGG ACCGTACCAT GGAGGAGATC GTGTTCCCAG TGCCAAACAT CTGCGAGTTC 
CTGACCACCG AATCCAAGCT GCGCGTCTAC TATACCACAG AGAGAGATGA GCAGGGCAGC 
AAGATCAATG ACTTCTTCTT GCGAGCGGAA GACCTCTTTA ATGAGATGAA CTGGCAGAAG 
AAGCTTAGAG CCCAGCCAGT GTTGTACTGG TGCTCCAGGA ACATGTCGGT CTGGAGTAAC 
ATTTGCTTTA ACCTGGCCTT ACTCATGAAC CTGCTGGTCT GCCTCTTCTA CCCGCTGGAA 
GGGGTGCAAG GAGTTATGCT GGATCCCCAC CTGTCTGCCC TGCTGTGGAT GGGGGTCGTT 
GCGGCCCTGG TCATTGTCCT CGTCATGCCT CAGCCGCTGA GCGTCCGCGC CCTCGTCATC 
ATCACCATCC TCCGCCTCAT CTTCTCGGTC GGCCTTGAGC CCACCCTCTT CCTTCTGGGC 
GCCTTGAATG TGTGCAATAA AATCATCTTC ATGATCAGCT TCGTGGGGAA CCGGGGAACC 
TTTACGCGCG GCTACAAAGC CATGGTGCTG GACTTTGAGT TCCTCTACCA CCTCATTTAC 
CTCATCATTT GCTGTCTAGG AGTGTTCGTC CACGTCTTCT TCTACAGCCT GCTGCTTTTT 
GACCTCGTGT ACCGAGAGGA GACGCTGCTG AACGTGATCA AGAGTGTGAC CCGCAACGGC 
CGCTCCATCG TGCTGACCGC CGTGCTGGCT CTCATCCTGG TCTACCTGTT CTCCATCGTC 
GGCTACATTT TCTTCAAGGA TGACTTCATC CTGGAGGTGG ACCGCGTCCC CAACGCAACT 
ACGAAAGGTG GAGGCAGTCT GGCCAGCGTG TTTGTTCCCG CTGGAATGTG TCAGGGAGGG 
ACTGATCAGA ACTGCACCGC AAGGGCCCAG CGGGAAGGTG ATCGGGACCG CGTCGGTGGT 
GACGGCGACG GGGACAAGGA CGAGGACGTG GAGCGGACGT GCGACTCCCT GCTGATGTGC 
ATCGTTACCG TCCTGAGTCA CGGCTTGAGG AGCGGAGGAG GAGTCGGTGA TGTTCTTCGC 
AAGCCTTCCA AGGAGGAGCC TCTGTTTGCC GCCAGAGTGA TATACGACTT GCTCTTCTTC 
TTCATGGTCA TCATCATCGT CCTCAACCTC ATCTTCGGTG TCATCATTGA CACCTTCGCT 
GACCTGAGGA GTGAGAAACA GAAGAAAGAG GAGGTTCTGA AAACGACCTG CTTCATCTGC 
GGTCTGGAGC GGGACAAGTT CGACAACAAG ACCGTGACGT TTGAGGAGCA CATCAAGGAG 
GAACACAACA TGTGGCACTA CCTGTTCTTT ATCGTGCTCG TGAAAGTTAA AGACTCCACT 
GAGTACACAG GCCCCGAGAG CTACGTGGCT GAGATGATAA AGGAGCACAA CCTGGACTGG 
TTCCCACGGA TGCGTGCCAT GTCTCTGGTG AGCAGCGACG CCGAGGGGGA GCAGAATGAA 
ATCAGGAACC TGCAGGAGAA GCTGGAGTCG ACCATGAGGC TGGTGGCCAA CCTGTCTGGA 
CAGCTGAGTG AGCTCAAGGA TCAGATGACA GAGCAGAGGA AACACAAGCA ACGGATCGGT 
CTCCTTGGAC ACCCCCCCCA CATGAACATC AATCCACAGC AACCAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011612036.1
CDS	255..8405
Translation	MSDKMSSFLHIGDICSLYAEGSTNGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGNTDAVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVMLDTAGNEGSWFYIQPFYKLRSIGDSVVIGDKVVLNPVNAGQPLHASTHQLVDNPGCNEVNSVNCNTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDDHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPEYEEEGPESRSPLELEQEALRARLRNVQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSTLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAGKLEKGTITQNERRAVTKLLEDLVFFVVDIPNSGQDVLEIMVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRHSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDQANADILIETKLVLSRFEIEGAPLGENSLESEEDEEEVWLFWKDNNNKEIRSKSIRELAQDAKEGQKEDQEVIGYYRYQLNLFARMCLDRQYLAINKISGQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSEIAIDDYDNDGTSKDEIKERFCQTMEFVENYLRDVVCQSFPFADKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHVGTIFGLNKLDKEDESKGSNVMKSIHGVGELMSHVVLRGGGFLPMTSSSSTSRDTVKTQTEPEKQDILVMDTKLKIIEILQFILNVRLDYRISCLLSIFKREFDESNSQSELTVGGAVEGPNNMPGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALQLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSEEGMDAGEPEHKLGDAVGTDKTKKPESTSSYNYRVVKEILLRLSKLCVQEGLSGRKSKKQQQRLLRNMGAHAVVLELLQIPYEKGEDLRMQDIMKLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAITMQHIFMNNFQLCSEINERVVQHFVHCIETHGRNVQYLKFLQTIVKAENKFIKKCQDIVMAELVNAGEDVLVFYNDRASFQTLVQMMRSERDRMDENSPLMYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKIAYINFLNHCYVDTEVEMKEIYTSNHMWKLFENFLVDICRVCNSTSERKHADTILERYVTETVMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQAIFRVYHCNWLIPVQKGSVESCIKVLSDVAKGRAIAIPVDLDNQVHNLFVKSNNIVQKTAMSWRLSARNAARRDSMVTASRDYRNIIERLQDIVSALEDRLRPLVQAELSVLVDVLHRPELLFPENTDSRKKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQILVNRYYGNFHRSGGRRDSLTFANGPLSPIGPGKSQSGLGGPGALSRGEMSLAEVQCHLDKEGASDLVIDLIMNTTSDRVFQESILLAIALLEGGNTTIQRSFFCRLTEDKKSEKFFRVFYDRMKLAQLEIKATVTVNTSDLGNRKREDDNQDKDGPVRKKARESSVVMTEDVREQLLEASSATKKAFNSYRREADPEDHFNVVDGQSSAGDKNQDEGEMSFVIVIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINEKNVALINQTLESLTEYCQGPCHENQNCIATHESNGIDIIIALILNDINPLGKKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVIKKAYLQGEVEIEDTKEEEENEEEDHDAASPRNVGHNIYILAHQLARHNKELAVMLKPGVAVGEGDEALECYAKHTAQIEIVRQDRTMEEIVFPVPNICEFLTTESKLRVYYTTERDEQGSKINDFFLRAEDLFNEMNWQKKLRAQPVLYWCSRNMSVWSNICFNLALLMNLLVCLFYPLEGVQGVMLDPHLSALLWMGVVAALVIVLVMPQPLSVRALVIITILRLIFSVGLEPTLFLLGALNVCNKIIFMISFVGNRGTFTRGYKAMVLDFEFLYHLIYLIICCLGVFVHVFFYSLLLFDLVYREETLLNVIKSVTRNGRSIVLTAVLALILVYLFSIVGYIFFKDDFILEVDRVPNATTKGGGSLASVFVPAGMCQGGTDQNCTARAQREGDRDRVGGDGDGDKDEDVERTCDSLLMCIVTVLSHGLRSGGGVGDVLRKPSKEEPLFAARVIYDLLFFFMVIIIVLNLIFGVIIDTFADLRSEKQKKEEVLKTTCFICGLERDKFDNKTVTFEEHIKEEHNMWHYLFFIVLVKVKDSTEYTGPESYVAEMIKEHNLDWFPRMRAMSLVSSDAEGEQNEIRNLQEKLESTMRLVANLSGQLSELKDQMTEQRKHKQRIGLLGHPPHMNINPQQPA

Target ORF information:

RefSeq Version	XM_011613734.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes inositol 1,4,5-trisphosphate receptor type 1 (LOC101074739), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011613734.2

ORF Insert Sequence:

ATGTCGGACA AGATGTCCAG CTTCCTGCAC ATTGGGGACA TCTGCTCGCT GTATGCAGAG 
GGCTCCACCA ATGGTTTCAT CAGCACTCTC GGCTTGGTGG ACGACCGCTG CGTCGTCCAG 
CCCGACGCCG GCGACCTCAA CAACCCCCCG AAGAAGTTCC GAGACTGCCT GTTCAGACTG 
TGTCCCATGA ACCGCTACTC GGCCCAGAAG CAGTTCTGGA AAGCGGCGAA GCCTGGAGGG 
AACACCGACG CTGTGCTCCT CAACAAGCTG CATCATGCCG CCGACCTGGA GAAGAAGCAG 
AATGACTCTG AGAACAAAAA GCTGCTTGGA ACAGTGATTC AGTACGGGAA CGTCATTCAG 
CTCCTCCACC TGAAGAGCAA CAAGTACCTG ACGGTGAACA AGCGTCTACC CGCTCTGCTT 
GAGAAGAATG CCATGAGGGT GATGCTGGAC ACAGCAGGAA ATGAGGGCTC CTGGTTCTAC 
ATCCAGCCCT TCTACAAACT GCGCTCCATC GGAGACAGCG TGGTGATTGG AGACAAAGTG 
GTTCTGAACC CAGTGAACGC CGGTCAGCCG CTGCACGCCA GCACGCACCA GCTGGTGGAT 
AATCCAGGAT GCAACGAGGT GAACTCGGTC AACTGTAACA CTAGCTGGAA AATCGTCCTG 
TTTATGAAAT GGAGCGATAA CCAAGAGATC GTTTTGAAAG GGGGTGACGT TGTGCGGTTA 
TTTCACGCAG AGCAGGAGAA GTTTCTCACG TGTGACGATC ACAGGAAAAA ACAGTATGTC 
TTCCTGCGCA CTACCGGACG TCAGTCTGCA ACGTCGGCCA CCAGCAGCAA AGCGCTGTGG 
GAAGTCGAGG TTGTGCAGCA CGATCCGTGT CGAGGGGGGG CTGGCTACTG GAACAGTCTG 
TTCAGGTTCA AGCACCTGGC CACTGGGCAC TACCTGGCTG CAGAGGTGAA TCCAGAGTAT 
GAAGAGGAGG GTCCGGAATC ACGATCCCCT CTGGAGTTGG AGCAGGAAGC CCTCCGAGCC 
CGGCTGAGGA ACGTTCAGGA CAAGGTCATG TACACCCTTG TGTCTGTCCC TGACGGGAAT 
GACATCTCTT CCATTTTTGA GCTCGACCCT ACCACGCTAA GAGGGGGGGA CTCCCTGGTG 
CCCAGGAACT CGTATGTGCG TCTCAGACAC CTCTGCACCA ACACATGGGT TCACAGCACC 
AATCAGCCAA TCGACAAAGA GGAGGAGAAG CCTGTCATGT TGCGTATTGG CACCTCTACT 
CTAAAAGAGG ACAAGGAGGC CTTTGCCATT GTGCCCGTCT CCCCTGCTGA GGTGAGAGAC 
CTCGACTTTG CTAATGATGC CAGTAAAGTG CTTGCTTCTA TTGCTGGGAA GCTGGAAAAA 
GGCACCATCA CGCAGAACGA GAGGAGAGCG GTCACGAAGC TTCTGGAGGA CTTGGTGTTT 
TTTGTGGTGG ATATTCCCAA CAGTGGCCAG GATGTTCTGG AGATCATGGT CAACAAACCC 
AACAGGGAGC GTCAGAAGCT CATGAGGGAA CAGAATATTC TCAAACAGAT CTTCAAGCTG 
CTGCAGGCGC CCTTCACAGA CAGTGGGGAC GGACCCATGC TGAGACTGGA GGAGCTGGCC 
GACCAGCGCC ATGCCCCCTT CAGACACATC TGCCGACTGT GTTACCGCGT TTTGCGTCAC 
TCTCAGCAGG ACTACCGTAA AAATCAGGAA TATATAGCCA AACAGTTCCG CTTCATGCAG 
AAGCAGATCG GCTATGACGT GCTGGCCGAG GACACAATCA CAGCTCTGCT CCACAACAAC 
CGAAAGCTGC TGGAGAAGCA CATCACGGCC GCTGAGATCG ACACCTTCGT CAGCCTGGTT 
CGCAAGAACC GCGAGCCTAG GTTCCTGGAC TACTTGTCAG ACTTGTGCGT GTCGATGAAC 
AAGTCCATCC CCGTGACCCA GGAGCTCATC TGCAATGCAG TGCTGGATCA GGCTAACGCA 
GACATCCTCA TAGAGACTAA GCTGGTGCTG TCGAGATTTG AGATCGAGGG AGCGCCGCTC 
GGAGAAAACT CACTGGAGTC GGAGGAGGAC GAGGAGGAGG TGTGGCTGTT CTGGAAGGAT 
AATAATAATA AAGAAATCCG CAGTAAAAGC ATTCGGGAGC TGGCCCAGGA TGCCAAAGAG 
GGTCAGAAGG AGGACCAGGA AGTGATCGGT TACTACAGAT ACCAGTTGAA CCTGTTTGCC 
AGGATGTGTT TGGACCGGCA GTACCTGGCA ATCAACAAGA TTTCCGGCCA GTTGGACGTG 
GACCTAATCT TGCGCTGCAT GTCGGACGAA GACCTGCCCT TTGACCTGCG AGCCTCGTTC 
TGCCGCATGA TGCTGCACAT GCATGTGGAC CGCGATCCAC AGGAACAAGT CACACCGGTC 
AAATATGCAC GGCTCTGGTC TGAGATCCCC TCTGAGATCG CCATCGACGA CTATGACAAC 
GATGGGACGT CCAAGGACGA GATTAAAGAG CGCTTCTGTC AGACCATGGA GTTTGTCGAG 
AACTACCTGA GAGACGTTGT CTGTCAGAGT TTCCCGTTCG CAGATAAAGA AAAGAACAAG 
CTCACGTTTG AGGTGGTGAA CCTGGCAAGG AACCTGATTT ACTTCGGCTT CTACAACTTC 
AGTGACCTGC TGAGACTCAC CAAGATCCTG CTGGCCATTC TAGACTGCGT CCACGTCGGC 
ACCATTTTCG GTCTCAACAA GCTGGACAAA GAAGATGAGA GTAAAGGCAG CAATGTGATG 
AAGTCCATTC ACGGTGTGGG AGAGCTGATG TCCCACGTCG TCCTGAGAGG AGGGGGCTTT 
CTCCCCATGA CTTCAAGCAG CAGCACCAGC AGAGACACAG TTAAAACCCA GACTGAACCC 
GAGAAACAGG ACATTTTGGT CATGGACACC AAGCTGAAGA TAATAGAGAT CCTGCAGTTC 
ATCTTGAACG TGCGGCTGGA CTACAGGATC TCCTGTTTGC TGTCGATATT TAAGAGGGAA 
TTCGACGAGA GCAACTCCCA GAGCGAGCTA ACTGTGGGCG GAGCCGTGGA AGGGCCGAAC 
AACATGCCAG GAGCTTTGGA TTTTGAACAC ATCGAAGAAC AGGCAGAAGG CATATTTGGT 
GGAAGTGAAG AAAACACACC ACTGGACCTG GATGATCATG GCGGCCGTAC CTTTTTGAGG 
GTCCTGCTCC ACTTAACCAT GCATGATTAT CCACCACTGG TATCTAGAGC GCTCCAGTTG 
CTCTTCAGGC ATTTCAGCCA AAGGCAGGAG GTTTTGCAGG CTTTCAAGCA GGTCCAGCTG 
CTCGTCACCA GCCAGGATGT GGAAAATTAC AAACAAATCA AGTCGGACCT TGACCAGCTT 
CGTTCCATCG TGGAGAAGTC CGAACTCTGG GTGTACAAGC GGCAGGGCTC CGAGGAGGGG 
ATGGATGCAG GAGAGCCTGA GCACAAACTG GGTGATGCAG TGGGCACAGA CAAGACAAAG 
AAACCCGAAA GCACCAGCAG TTACAATTAC AGGGTGGTGA AGGAGATCCT GCTGCGGCTC 
AGCAAGTTGT GTGTCCAAGA AGGTCTGTCA GGCAGGAAGA GCAAGAAGCA GCAGCAGAGG 
CTGCTGAGGA ACATGGGGGC CCACGCCGTT GTTCTCGAGC TGCTTCAGAT CCCATACGAG 
AAGGGAGAAG ATTTGCGGAT GCAGGACATC ATGAAATTAG CCCACCAGTT TCTACAGAAC 
TTTTGTGCTG GGAACCAGCA GAACCAAGCC CTGCTGCACA AGCATATCAA TCTCTTCCTC 
AACCCCGGGA TTTTGGAAGC CATCACCATG CAGCACATCT TCATGAACAA CTTCCAGCTG 
TGCAGCGAGA TAAACGAGCG CGTGGTTCAG CACTTTGTCC ACTGCATCGA GACGCACGGG 
CGCAACGTTC AGTATCTGAA ATTCCTTCAG ACTATCGTCA AAGCGGAGAA CAAGTTCATC 
AAAAAGTGTC AGGACATCGT CATGGCTGAG CTCGTAAACG CCGGCGAGGA CGTTTTGGTC 
TTCTACAACG ACCGCGCCTC CTTCCAGACA CTGGTGCAGA TGATGCGATC GGAGCGGGAC 
CGCATGGATG AAAACAGTCC CCTGATGTAC CACATCCACC TAGTAGAGCT ACTGGCCGTG 
TGCACGGAGG GCAAGAATGT CTACACAGAG ATCAAGTGCA ACTCCTTGCT ACCTCTAGAT 
GACATCGTGC GGGTGGTGAC GCATGAGGAC TGTATTCCCG AGGTGAAGAT CGCTTACATC 
AACTTCCTAA ACCACTGCTA TGTCGACACG GAAGTGGAGA TGAAAGAGAT CTACACCTCC 
AACCACATGT GGAAACTCTT TGAGAACTTT CTGGTCGACA TCTGCCGGGT GTGTAACAGC 
ACGAGCGAGC GCAAGCACGC CGACACCATT CTGGAGCGCT ACGTGACGGA GACGGTCATG 
AGCATCGTAA CGACGTTCTT CAGCTCGCCA TTCTCGGACC AGAGCACCAG CCTGCAGACC 
AGACAGCCTG TCTTTGTTCA GCTATTGCAG GCGATTTTCA GGGTGTACCA CTGTAACTGG 
CTGATCCCAG TTCAGAAGGG CTCTGTAGAG AGCTGCATTA AGGTGCTCTC TGATGTTGCC 
AAAGGTCGAG CAATAGCCAT CCCGGTGGAC CTGGACAACC AGGTGCACAA CCTTTTTGTG 
AAATCCAACA ATATTGTTCA GAAGACGGCC ATGAGCTGGA GACTTTCGGC CCGCAATGCT 
GCCCGCAGGG ACTCGATGGT GACTGCCTCA CGGGACTACA GGAACATCAT AGAGAGACTA 
CAGGACATCG TGTCAGCACT GGAGGACCGT CTGCGCCCGC TGGTCCAGGC CGAGTTATCG 
GTCCTGGTGG ACGTGCTGCA TCGGCCTGAG CTGCTCTTCC CTGAAAACAC CGACTCGCGC 
AAGAAGTGCG AGAGCGGAGG TTTTATCTGC AAGCTGATCA AACATACCAA GCAGCTGCTG 
GAGGAAAATG AAGAGAGGCT GTGCATCAAA GTCCTGCAAA CTCTGCGAGA GATGATGACA 
AAAGACCGAG GCTATGGAGA GAAGGGCGAG GCGCTCAGAC AGATTCTGGT GAACCGTTAC 
TATGGCAACT TCCACCGGAG CGGCGGACGC AGGGACAGCC TGACCTTTGC CAACGGCCCG 
CTCAGTCCGA TCGGGCCTGG CAAAAGCCAA TCAGGCTTGG GGGGTCCTGG GGCCTTGAGC 
CGTGGGGAGA TGAGCCTGGC GGAGGTTCAG TGTCACTTGG ACAAGGAGGG AGCCTCGGAC 
CTGGTGATCG ATCTCATCAT GAACACCACA AGCGACCGAG TCTTCCAAGA AAGCATCCTG 
CTGGCTATCG CACTGCTCGA AGGTGGAAAC ACAACCATCC AGCGGTCATT CTTTTGTCGC 
CTGACTGAAG ACAAGAAGTC AGAGAAGTTC TTCCGGGTCT TCTACGACCG TATGAAGCTG 
GCTCAGCTGG AGATCAAAGC CACTGTGACG GTAAACACCA GCGATTTAGG AAACAGGAAG 
CGAGAAGATG ACAACCAGGA CAAAGACGGC CCTGTTCGTA AAAAAGCCAG AGAATCTTCT 
GTGGTCATGA CGGAGGATGT GCGAGAACAG CTGTTAGAAG CCTCTTCTGC CACCAAGAAA 
GCCTTCAACT CTTATCGGCG TGAAGCAGAC CCCGAGGACC ACTTCAACGT AGTGGACGGC 
CAGTCCAGCG CGGGAGACAA GAACCAGGAT GAAGGAGAAA TGAGCTTTGT TATCGTCATC 
ATGCAGCCGA TCCTTCGCTT CCTTCAGCTG CTGTGTGAGA ACCACAACCG GGATCTGCAG 
AACTTCCTCC GCTGTCAAAA TAACAAGAAC AACTACAACC TTGTGTGCGA GACCCTTCAG 
TTCTTGGACT GTATCTGTGG CAGCACCACG GGGGGCCTCG GCCTACTGGG CCTTTACATC 
AATGAGAAGA ATGTGGCCCT CATTAACCAA ACCCTGGAGA GTCTGACTGA GTACTGTCAG 
GGCCCCTGCC ATGAAAACCA GAATTGTATC GCCACCCATG AGTCCAATGG AATTGACATT 
ATCATCGCCC TGATTCTTAA CGACATCAAC CCACTTGGGA AGAAGCGAAT GGACCTGGTG 
CTGGAGCTGA AGAACAATGC GTCCAAGCTG CTGCTGGCAA TCATGGAGAG TCGACATGAC 
AGTGAGAATG CAGAGAGGAT CCTGTATAAT ATGAGACCCA AAGAGCTGGT GGAGGTGATA 
AAGAAGGCTT ACTTACAAGG AGAGGTGGAG ATCGAGGACA CAAAAGAGGA GGAAGAGAAT 
GAAGAGGAAG ATCACGACGC TGCTTCTCCC CGAAACGTTG GACACAACAT CTATATTTTA 
GCTCACCAGT TGGCGCGTCA CAATAAGGAG CTGGCGGTGA TGTTAAAGCC AGGCGTGGCC 
GTTGGGGAGG GAGATGAGGC CCTGGAGTGT TACGCCAAAC ACACCGCTCA GATCGAGATT 
GTGCGTCAGG ACCGTACCAT GGAGGAGATC GTGTTCCCAG TGCCAAACAT CTGCGAGTTC 
CTGACCACCG AATCCAAGCT GCGCGTCTAC TATACCACAG AGAGAGATGA GCAGGGCAGC 
AAGATCAATG ACTTCTTCTT GCGAGCGGAA GACCTCTTTA ATGAGATGAA CTGGCAGAAG 
AAGCTTAGAG CCCAGCCAGT GTTGTACTGG TGCTCCAGGA ACATGTCGGT CTGGAGTAAC 
ATTTGCTTTA ACCTGGCCTT ACTCATGAAC CTGCTGGTCT GCCTCTTCTA CCCGCTGGAA 
GGGGTGCAAG GAGTTATGCT GGATCCCCAC CTGTCTGCCC TGCTGTGGAT GGGGGTCGTT 
GCGGCCCTGG TCATTGTCCT CGTCATGCCT CAGCCGCTGA GCGTCCGCGC CCTCGTCATC 
ATCACCATCC TCCGCCTCAT CTTCTCGGTC GGCCTTGAGC CCACCCTCTT CCTTCTGGGC 
GCCTTGAATG TGTGCAATAA AATCATCTTC ATGATCAGCT TCGTGGGGAA CCGGGGAACC 
TTTACGCGCG GCTACAAAGC CATGGTGCTG GACTTTGAGT TCCTCTACCA CCTCATTTAC 
CTCATCATTT GCTGTCTAGG AGTGTTCGTC CACGTCTTCT TCTACAGCCT GCTGCTTTTT 
GACCTCGTGT ACCGAGAGGA GACGCTGCTG AACGTGATCA AGAGTGTGAC CCGCAACGGC 
CGCTCCATCG TGCTGACCGC CGTGCTGGCT CTCATCCTGG TCTACCTGTT CTCCATCGTC 
GGCTACATTT TCTTCAAGGA TGACTTCATC CTGGAGGTGG ACCGCGTCCC CAACGCAACT 
ACGAAAGGTG GAGGCAGTCT GGCCAGCGTG TTTGTTCCCG CTGGAATGTG TCAGGGAGGG 
ACTGATCAGA ACTGCACCGC AAGGGCCCAG CGGGAAGGTG ATCGGGACCG CGTCGGTGGT 
GACGGCGACG GGGACAAGGA CGAGGACGTG GAGCGGACGT GCGACTCCCT GCTGATGTGC 
ATCGTTACCG TCCTGAGTCA CGGCTTGAGG AGCGGAGGAG GAGTCGGTGA TGTTCTTCGC 
AAGCCTTCCA AGGAGGAGCC TCTGTTTGCC GCCAGAGTGA TATACGACTT GCTCTTCTTC 
TTCATGGTCA TCATCATCGT CCTCAACCTC ATCTTCGGTG TCATCATTGA CACCTTCGCT 
GACCTGAGGA GTGAGAAACA GAAGAAAGAG GAGGTTCTGA AAACGACCTG CTTCATCTGC 
GGTCTGGAGC GGGACAAGTT CGACAACAAG ACCGTGACGT TTGAGGAGCA CATCAAGGAG 
GAACACAACA TGTGGCACTA CCTGTTCTTT ATCGTGCTCG TGAAAGTTAA AGACTCCACT 
GAGTACACAG GCCCCGAGAG CTACGTGGCT GAGATGATAA AGGAGCACAA CCTGGACTGG 
TTCCCACGGA TGCGTGCCAT GTCTCTGGTG AGCAGCGACG CCGAGGGGGA GCAGAATGAA 
ATCAGGAACC TGCAGGAGAA GCTGGAGTCG ACCATGAGGC TGGTGGCCAA CCTGTCTGGA 
CAGCTGAGTG AGCTCAAGGA TCAGATGACA GAGCAGAGGA AACACAAGCA ACGGATCGGT 
CTCCTTGGAC ACCCCCCCCA CATGAACATC AATCCACAGC AACCAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101074739 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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