LOC101071632 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101071632
Entrez Gene ID	101071632
Full Name	piezo type mechanosensitive ion channel component 1
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011619640.1	XP_011617942.1	LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel component 1
XM_029832472.1	XP_029688332.1	piezo-type mechanosensitive ion channel component 1

Pathways from BioSystems
Gene Ontology

The following LOC101071632 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101071632 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo26286	XM_011619640.1 Latest version!	Takifugu rubripes piezo-type mechanosensitive ion channel component 1 (piezo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OTo40515	XM_029832472.1 Latest version!	Takifugu rubripes piezo-type mechanosensitive ion channel component 1 (LOC101071632), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101071632 ( XM_011619640.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011617942.1 Takifugu rubripes piezo-type mechanosensitive ion channel component 1 (piezo1), mRNA.

Price & Availability↑

CloneID	OTo26286
Clone ID Related Accession (Same CDS sequence)	XM_011619640.1
Accession Version	XM_011619640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	LOW QUALITY PROTEIN: piezo-type mechanosensitive ion channel component 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004072440.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTATGCTA AAATATTTAA AAAGTATCTG CTGTTGTTTG TCTCTCTTTT TGCAGCCTGC 
CTTTTTCGCT ACAACCTCCT GTCTCTGATC TACTTTGTCT ACCTACTGCT GCTGCCATGG 
TTCCAGTGTC CCAATAAACA CACAATCAAA GGTCACACAG GAAACTTTAT TCGAGCCGTA 
TTTTTTACCA GTCTGGTGTT TGTCCTGGCA CATATATGTT TCCAGACGGT ACTATACACA 
TATCCTCCCC TCAGCCTTGC CCTAGGCCAT AATTGTTCAC AATGGGATGT CATCTCCAGA 
CATATAGGAT TGTCCAGGCT TCCCCTGGAT GAATATTTGA TTGTGTTGCG CCTGGTGTGT 
CCTGACCTGG GAGTTTTTGC CGTTTCCTTG GTAACAATCA TTGTCTGTAA CAGACTGGTC 
AAGAATAGAG AAACAGTTTC TGCTGCCAAC ATCACATCGT TAGAAGATAA TTCAGACTGC 
ACACTCACAG AGGATGAAGA TGATGTAAAT GATGATGATC ATCTCGAAGA AGAGGAGGAC 
ACAGCCAGTG ATAAAGATGA AGAGAGGACT TTACACTGGG ATTCTGAAGA AGAGGTTTCC 
ACAGTAACAA GAGCCAAGCA ACTGGCCGAG CAGTTCAAGG CCACGGCCTG GAAGGTTCTG 
TGGGACCTCA GGAGAATCTT GGCCATCAGC TTGTTGGCTT TGGCTGGTAT CACCATGCCT 
TCAGCCTTTA CAGCCATCTA TTTCTTGCTG TTTATATGTT TGTGCACGTG GTGGGCCTTC 
CACCTCCCCA TCAGCCATCT GGGATTCAAC GCCCTGTGTG TGATGTTGAG CTTCTTCACA 
TCTGGCCACA TGGTTTGTTT GTACTTGTAC CAGAGTGTGT TGGCCCAGGC ACTGTTCCCG 
CCCCCCTCTC TGTGGGCTAG ACTTTTTGGC CTGAAGGACA TCATCCAACC TGGAAACTGC 
TCCTCACCCT ATGACATGAC ACTCAATGTC AGTTATGAGT GGCCAGTTTA TGTCAGTCCG 
GGGATCCTCT TCCTGCTCTA CATAACCTTA GCAGCTGTCC TCAAGACGGA AAGCTATGGA 
AAAGCAAACC AGGAGCAAAC GCAGGAGGAA ACAGGCGCAT CAACGGAGGA CATGCTGGAG 
TTAAAGACAT GGAACAAACA CACAAAAAGG ACGACGACGA CTCAGAGTGC GTCCTTTTGT 
CCGTGGGAAC AGAGACATGC AGCAGCATCG AGGAAAAGCC TCCAGAGGGA CTCCTGCAGC 
CAGAGCTGGG AGGCTCCTGT CACCCACCCA GGGGAGAGCC CCTTCAGTCT GCTGGGGAGG 
GTGATCATGC AGCAGAGCTA CGTCTGTGCT CTCATCGCCA TGATGGTGTG GAGCATCATG 
TACCACAGCT GGCTGACTTT TGTGCTACTA CTGTGGGCTT GTCTCATCTG GATCCTGCGC 
ACAAGGCGTC AAGCCGCCAC CTTGTGTTCC CCGTTCATCC TGCTTTATGG TCTGGCTCTT 
TGCTGTCTGC AGTACATCTG GGCTATTGAG CTCCAGCCTG AATTACCCAC CACACTAGGA 
ACCATGAGAC TCAACCAGCT GGGACTAGAT AGAGCCAAGT ACCCCTGTCT CAGGCTGGGA 
GCTATGGTTC TCTTCACTCT GACATTCTGG CTGCTCGTAC GTCAGTCAGT GAAGGAGAAC 
TTCAGCAAGA AGAGAAAAGT GGTCACACCT CTAGAAGATG TCCGGACAGA GGAGAAAGGT 
GAGAAGGAAC CGGTGCTGAA GGTTGTGGGA GCCCTGGTGA TGAGCTGCTA TGCCAAATAT 
TGGATCTTTG TGTGCGGAGG AATGTTCATC ATGGTGTCGT TTGCTGGGAA ACTTGTTGGA 
TACAAGATTG TCTACATGCT GATGTTCTTG ATCTGCCTCT GTCTGTATCA GGTCTGTTAC 
TCTCTATGGA GGCGTCTGTT GAAGCTTTTC TGGTGGCTTG TCGTGGCCTA CACCATGCTG 
GTGCTCATCT CCATCTACAC GTACCAGTTT GAAGACTTCC CTGGATACTG GAGAAACTTT 
ACTGGTTTCA CGGAACAACA GTTAGAAGCT ATTGGTTTGG AGACGTTTGC TCTATCGGAA 
CTCTTCACCA GCATTCTGAT TCCGGGCTTC TTCCTGCTGG CCTGTATTCT GCAGCTGCAC 
TACTTCCACA AACCCTTCAT GCGAATCACC GACCTAGAGG ACGTTATGCC GTTACCAAGG 
AAGGAGAAGA AGTTTTTAAG GACGGAGCTT CCTCACTCCG ATGATGAAGA GGAGGAACTG 
GTTGTTAGCA ATGAGGATTC TGATGATGAA GTGGAGCTCA CGCCCAGTAA GTGGGGTCTG 
GTAATGGACA GGTTACTGGT TCTGTCCAGA CGGTTCTCTG ACACTCTCAC TAAAGTTCAA 
GAGTTTGTCT GGAGGCTTCT GGAGCTCCAC ATTGCAAAGA TGGTGGCCCT GTTCGCTGTG 
TGGGTGGCCC TTGAAGAGCC ATCATTGATG AACCTGGTCC TGGTGGTCTT GTGGACCCTG 
GCGATGCCCT ACAGCGCCTT CAGGCCCATG ACTTCCTGTC TGTCCACAGT CTGGGTCTGT 
GTCATCATCG TGTGTAAAAT GCTGTACCAG CTCAGTGTTG TCAACCCCGT AGAGTACTCC 
AGTAACTGCA CCAAGCCTTT ATCAAATGAA ACGAACCTTT CACCAGAGGA GATATTAGAC 
TCTTCTCTAT ATAAAGAGCC GGTGGACCCT GCGAGATGGT TCGGTATCAG GAAGGACGCC 
ACCATCCTGG GATACAGCCA GAGTCACCTG TTATTGCTGA TGATGCTGGT GTTCGAGGCT 
ACAGTCTACC GTCACCAAGC TCACCACTAC CGCCAGCTCC AAAAGTCTCC TCCTACCATC 
CCAACTCTGT TTGCCACCGC CACAAGGGAC ACTCTGGACC AGGGCCTCAT CCCCTGCCTC 
AAGTACCTGC TTAACTACAC CTTTTACAAG TTTGGATTAG AGATTTGTTT CCTGATGATG 
GTGAATGTGA TTGGCCAGAG GATGAACTTC TTAATGATAA TCCATGGCTG TTGGCTGGTG 
GTCATCTTGT CGAAGCGCCG GCGGGCAGCT ATTGCCAGGG TCTGGCCCAG CTATTGTGTC 
TTCCTGTCTG TCTTCATGAT CTACCAGTAT CTGCTGTGTT TAGGCATACC GCCCGCTCTG 
TGCATAGAGT ACCCATGGAG ATGGAAGACT CCAGTCCTGA TCAACTCAGC TCTTATAAAA 
TGGATCTATC TGCCCGACTT TTACACAGTA CCTAACTCCA GAAACCTCAT CGCGGATTTT 
GTGTTGCTGA TGTGTGCGTC CCAGCAGTGG AAGGTGTTTG AGTGTGAGCG CACAAGGGAG 
TGGACGGTTC TGGCAGGAGA GAACGTAGAT GACCCAGAAC ACGTGGACGG TCGACTCTGT 
AATCCGGCAG CAAATTTCAT CAACTGCAGG AGTTACCTGG ACATGGCTAA AGTTCTGGTG 
TTTCGACACT TGTTCTGGTT TGTGCTGTCA GTGGTCTTCA TTACTGGAGC AACCAGAATC 
TCGGTGTTTG GGCTTGGATA CCTCCTGGCC TGTTTTTTTT TCCTTCTGTT CGGGACTCAT 
TTCTTGGTCA AGCCCTGCAG GACCCGCCTC AACCTGTGGG ACTGTCTCAT CATCTATAAT 
GTGGCAGTCA TTATATCCAA AAATATGTTA TCAATTCTGG CGTGCGTGTT TGTTTCTGCG 
ATGCAGAATG GCTTCTGTTG GGTGATTCAA CTCTTCAGCC TGGTTTGCAC AGTCAATGGA 
TACTATGACC CAAAAGCAGT GAATGACAAG AACTGCAGTC TCCCAGTGGG AGAGGCCGGG 
ATTCTCTGGG ACAGTATCTG CTTCTTCTTC CTCTTGCTTC AGAGGAGGGT CTTTCTCAGT 
TTCTACTTCC TACATGTTGT CGCTGAGCTA CAGGCTTCTG CTAAACAAGC CTCCAGGGGG 
TTTGAACTCT TCAAAGCCAG TATTGTGAAG AACACGAATA TCTACCAACA AGCTGAGGAG 
AAATCGCTGT CTCAGCTCAA AAGATCAATG CAGAGAATTC GTTGCAGACA GAAGAAGTAC 
AGAGCTGGAC TGAGAGGAAG CGACGAGTCC TCAGAACCCG ACACTGCTGG TTGCCCGGGT 
CACTCCACAC CTGAAGCAGA ACTTGTTGAC ACAGGTGACA TTGGAGCCGA CCCCCACATG 
GACAAGAAGT TGAGGAAGAA TAAGGGGTAT CACCCCTGGT GGGACCATGC CACAGCCCGC 
CACGCAGGAG ATTATTACCT TTTTGAATCC GAAAGTGAAG ATGAAGAAGA GTTACAGTCT 
GAAGAACAGA AGCCTCCAAA ACAGACGGCA TGTCAGCTAG CGTACCAAAC ATGGGTGAGC 
AGCGTGAAAA CAGCTCTCAG AGAACGTCAG AGTCTGCAAA GACATCTAAG GACTAAGAAA 
CCAGAAAAGG ACTCACAAAA CACAACAGGC TGTGGGAACA GTGATGGGTT TGAGGATTCT 
GATAATCCGG AGGAGGAAAA GCCATCTGCC CACGGTGAGA TGGTCCAGAG GATCTTAGAT 
CTTCTGCATT TTTTGTGGGC CATCGTTTTG GCCATGGTCG ATGGGATGAC TCTGTGGTTA 
AACCTGCTGA CCAAACAGTA CCGAGAAATG TCTGTCGTTC TGTGCAATGA ACGCTACTTC 
ATAACACACA AGATACAGCA GCATCACACA ACAGAGTCAA CCAATGGGAA GTTATCTGAA 
GACAGGGACA GTTCCAGGCT GGAGCTGGTC TTTGGTGAAG CTGAAGCAGC AAAATCAGCA 
GGATACAGCT GCTTGGAGGT ATGTAGTGGG GAGTTGACAG AGCAGTCGCC CCCCACTGCA 
AGTGCCATCA GCAGGAGTCC AGAAGCCCAG AGCAGCAGCA TGGAGCTGCT GGTCTGCCCA 
TCAAAACAGC AAAGACACAA CAGAACAGCC AGCGAGCTCC TGGACGACAG AGAGTCCTTC 
ATTGAGGAGC TGGAAGACAG CAGATCATTC TTCAAAAGCC AGAATCGTCT GCTGAAGCTG 
CTGTTTGCCC TGTACAACCT GCTGGCTGCC AACTCAGAAG TAGCCTGTTA CTTAATCATC 
GTGCTGAACA ACATGGTCAC TGCCTCGGTC ATCTCCCTCA TTCTGCCCAT CTTGGTTTTC 
CTTTGGGCGA TGTTGTCTGT GCCGAGGCCA ACCAAGAGAT TTTGGATGAC AGCTATTGTC 
TACACAGAGG TTATGGTGGT TGTAAAATAC TTTTTCCAGT TTGGATTCTT TCCTTGGAAC 
AGTGCCTATG AAATGGCAGT GAATGATGAT AAACCGTTCT TCCCACCTCG TATCCTGGGC 
CTCGAGAAGA CCGACAACTA CATCAGATAT GATCTTCTTC AGCTACTGGC ACTGTTCTTC 
CACAGGTCCC TGCTCAAGCG TTATGGCCTG TGGGACCACG AGGGACCCTT AGAGGAGCAG 
AGTCCTACAC CAGACAACGT GGAGTCAGCT GATTCAAATG TCAGCGCTGC TGCTGAGATT 
CCACAATCGG CTCCAAAGGA ACCAATAAAC ACCGGGTCAG AAGATGGACA GGAACCTCCA 
TCGGTGCCAG AGGACATGTT GGAATCTGAT GGTAGTTGGC AGGAAGCAAG AACGGAAGAA 
GACCCCAAAC AAGAAGTGAA AAGCACTTTT ATCCGCTTTC GCAAGAAAAT TCAACATCCC 
AAAGGACCAC GCAGCAACGC GCCCCCTGCT GACACCACTG ACGCCACTAA AGCACCAACA 
AGAAAGAGAA AGAAAGTGAC GAAGGGAGAA ACGGGAGGTC AGAGATTGGT GGCGACAGTA 
CAAAAGATCA AACACGTCTT CGTCTCCGGG ATTCAGAGAG TTTACCGTCC AGCCAAATGT 
TTCTTTGGGA GCATCCTCCG TGCTGAGTAT CGGGCTCCCA CTGACGTCTA CGCACTCATG 
TTCCTCACTG ACGTCGTCGA CTTCATCGTC ATCATCTTTG GATTTTGGGC TTTTGGGAAA 
CACAGCGCCG CCGCTGATAT CGCCTCCACG CTGTCGGACG ACCAGGTCCC GGAGGCTTTT 
CTGGCGATGC TGCTCATCCA GTTCAGCACC ATGATCATCG ACAGAGCGTT GTACCTGCGC 
AAGGCTGTCT TAGGAAAGCT CATCTTCCAG GTGATCCTGG TGTTTGGGAT CCACCTGTGG 
ATGTTCTTTA TCCTGCCGGC TGTCACAGAA AGGATGTTCA ATCAGAACTT TGTCGCTCAG 
CTCTGGTACT TTGTCAAGTG CATTTACTTC GGCCTGTCGG CCCTCCAGAT CCGCTGTGGA 
TACCCGACTC GTATCCTGGG AAACTTCCTC ACCAAGAATT ACAACCACCT CAACCTGTTC 
CTTTTCCAAG GGTTTCGGCT GGTGCCCTTC CTGGTGGAGC TGCGGGCTGT GATGGACTGG 
GTCTGGACTG ACACCACTCT GTCTCTGTCA AACTGGATGT GTGTGGAGGA CATATACGCC 
AACATTTTTA TCATTAAATG CAGCCGTGAA ACAGAGAAGA AATACCCACA GCCTAAAGGA 
CAGAAGAAGA AGAAAATAGT CAAATATGGG ATGGGAGGAC TGATCATTTT CTTCTTGATC 
TGCATCATTT GGTTTCCTCT TCTTTTCATT TCGCTGGTCA GATCGGTGGT GGGCGTGGTC 
AACCACCCCA TCGACGTGAC CGTCACCATC AAGCTGGGAG GGTACGAGCC CCTCTTCACC 
ATGAGTGTGC AGCAACAGTC CATCCAGCCC TTTACAATGG TTGAATATGA ACAACTCACA 
AAGAAGTTTG GGAACAATGC GGCGGCCATG CAGTTTATAA CTCTGTATAA TTATGAGGAC 
ATTGTGACGG CCATGATTGA AGGTAGTTCT GGATCAGTGT GGAGGATCAG CCCCCCGAGC 
AGACAGGAAG TCATAAAGGA ACTGCTGAGT AGCCGTGCTG ACCTCACGCT GCGCCTGGCC 
TGGAATTTCC AAAGGGACCT GGGCAAGGGA GGGACGGTTG AACACTCCTT TGACAAGCAC 
TCAGTCCCTC TGGAGCCAGG GAACCCCGTC AGGGCTGATA TGGCCTCGTT GCTGCTCGGC 
AATCGTACCG AGCCGGTCCA CGTCCCTAAT TTGTTCCCGA ACTATCTACG TGCCCCCAAT 
GGAGCTGAAG CCAAACCTGT CAGTCAGCTA TTTAAAGGTC AGAAATACAT TGAAAATGGA 
TGGATTATTG ACAGGTCCCT GAACGGGGGG GTGAACCAGG AGTGGTGGGA TATTGCCATT 
GAAGGTTGTG CCCCCTCCTC CTGTGGGGTT CTGCCCATGG TCATATTTAA TGACAAAGTC 
AGTCCACCAA GTCTGGGCTT CCTTGCTGGA TATGGGATCA TGGGTTTGTA TGTTTCCGTG 
GTCTTAGTCA TCGGGAAATT TGTGCGTGGC TTCTTCAGTG AAATCTCACA CTCCATTATG 
TTTGAGGAGT TGCCCTGTGT GGACCGAATC CTGAAGCTGT GCACCGATAT CTTCCTGGTT 
CGTGAGACAG GAGAGCTGGA ACTAGAAGAG GAACTTTACT CTAAGCTGAT CTTCCTCTAT 
CGCTCTCCAG AGACGATGAT CAAATGGACC AGGGACATTA ATGCCCAAGA CCAGAATCCA 
GGAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011617942.1
CDS	1..7629
Translation	MYAKIFKKYLLLFVSLFAACLFRYNLLSLIYFVYLLLLPWFQCPNKHTIKGHTGNFIRAVFFTSLVFVLAHICFQTVLYTYPPLSLALGHNCSQWDVISRHIGLSRLPLDEYLIVLRLVCPDLGVFAVSLVTIIVCNRLVKNRETVSAANITSLEDNSDCTLTEDEDDVNDDDHLEEEEDTASDKDEERTLHWDSEEEVSTVTRAKQLAEQFKATAWKVLWDLRRILAISLLALAGITMPSAFTAIYFLLFICLCTWWAFHLPISHLGFNALCVMLSFFTSGHMVCLYLYQSVLAQALFPPPSLWARLFGLKDIIQPGNCSSPYDMTLNVSYEWPVYVSPGILFLLYITLAAVLKTESYGKANQEQTQEETGASTEDMLELKTWNKHTKRTTTTQSASFCPWEQRHAAASRKSLQRDSCSQSWEAPVTHPGESPFSLLGRVIMQQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWILRTRRQAATLCSPFILLYGLALCCLQYIWAIELQPELPTTLGTMRLNQLGLDRAKYPCLRLGAMVLFTLTFWLLVRQSVKENFSKKRKVVTPLEDVRTEEKGEKEPVLKVVGALVMSCYAKYWIFVCGGMFIMVSFAGKLVGYKIVYMLMFLICLCLYQVCYSLWRRLLKLFWWLVVAYTMLVLISIYTYQFEDFPGYWRNFTGFTEQQLEAIGLETFALSELFTSILIPGFFLLACILQLHYFHKPFMRITDLEDVMPLPRKEKKFLRTELPHSDDEEEELVVSNEDSDDEVELTPSKWGLVMDRLLVLSRRFSDTLTKVQEFVWRLLELHIAKMVALFAVWVALEEPSLMNLVLVVLWTLAMPYSAFRPMTSCLSTVWVCVIIVCKMLYQLSVVNPVEYSSNCTKPLSNETNLSPEEILDSSLYKEPVDPARWFGIRKDATILGYSQSHLLLLMMLVFEATVYRHQAHHYRQLQKSPPTIPTLFATATRDTLDQGLIPCLKYLLNYTFYKFGLEICFLMMVNVIGQRMNFLMIIHGCWLVVILSKRRRAAIARVWPSYCVFLSVFMIYQYLLCLGIPPALCIEYPWRWKTPVLINSALIKWIYLPDFYTVPNSRNLIADFVLLMCASQQWKVFECERTREWTVLAGENVDDPEHVDGRLCNPAANFINCRSYLDMAKVLVFRHLFWFVLSVVFITGATRISVFGLGYLLACFFFLLFGTHFLVKPCRTRLNLWDCLIIYNVAVIISKNMLSILACVFVSAMQNGFCWVIQLFSLVCTVNGYYDPKAVNDKNCSLPVGEAGILWDSICFFFLLLQRRVFLSFYFLHVVAELQASAKQASRGFELFKASIVKNTNIYQQAEEKSLSQLKRSMQRIRCRQKKYRAGLRGSDESSEPDTAGCPGHSTPEAELVDTGDIGADPHMDKKLRKNKGYHPWWDHATARHAGDYYLFESESEDEEELQSEEQKPPKQTACQLAYQTWVSSVKTALRERQSLQRHLRTKKPEKDSQNTTGCGNSDGFEDSDNPEEEKPSAHGEMVQRILDLLHFLWAIVLAMVDGMTLWLNLLTKQYREMSVVLCNERYFITHKIQQHHTTESTNGKLSEDRDSSRLELVFGEAEAAKSAGYSCLEVCSGELTEQSPPTASAISRSPEAQSSSMELLVCPSKQQRHNRTASELLDDRESFIEELEDSRSFFKSQNRLLKLLFALYNLLAANSEVACYLIIVLNNMVTASVISLILPILVFLWAMLSVPRPTKRFWMTAIVYTEVMVVVKYFFQFGFFPWNSAYEMAVNDDKPFFPPRILGLEKTDNYIRYDLLQLLALFFHRSLLKRYGLWDHEGPLEEQSPTPDNVESADSNVSAAAEIPQSAPKEPINTGSEDGQEPPSVPEDMLESDGSWQEARTEEDPKQEVKSTFIRFRKKIQHPKGPRSNAPPADTTDATKAPTRKRKKVTKGETGGQRLVATVQKIKHVFVSGIQRVYRPAKCFFGSILRAEYRAPTDVYALMFLTDVVDFIVIIFGFWAFGKHSAAADIASTLSDDQVPEAFLAMLLIQFSTMIIDRALYLRKAVLGKLIFQVILVFGIHLWMFFILPAVTERMFNQNFVAQLWYFVKCIYFGLSALQIRCGYPTRILGNFLTKNYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIIFFLICIIWFPLLFISLVRSVVGVVNHPIDVTVTIKLGGYEPLFTMSVQQQSIQPFTMVEYEQLTKKFGNNAAAMQFITLYNYEDIVTAMIEGSSGSVWRISPPSRQEVIKELLSSRADLTLRLAWNFQRDLGKGGTVEHSFDKHSVPLEPGNPVRADMASLLLGNRTEPVHVPNLFPNYLRAPNGAEAKPVSQLFKGQKYIENGWIIDRSLNGGVNQEWWDIAIEGCAPSSCGVLPMVIFNDKVSPPSLGFLAGYGIMGLYVSVVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCTDIFLVRETGELELEEELYSKLIFLYRSPETMIKWTRDINAQDQNPGI

Target ORF information:

RefSeq Version	XM_011619640.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes piezo-type mechanosensitive ion channel component 1 (piezo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011619640.1

ORF Insert Sequence:

ATGTATGCTA AAATATTTAA AAAGTATCTG CTGTTGTTTG TCTCTCTTTT TGCAGCCTGC 
CTTTTTCGCT ACAACCTCCT GTCTCTGATC TACTTTGTCT ACCTACTGCT GCTGCCATGG 
TTCCAGTGTC CCAATAAACA CACAATCAAA GGTCACACAG GAAACTTTAT TCGAGCCGTA 
TTTTTTACCA GTCTGGTGTT TGTCCTGGCA CATATATGTT TCCAGACGGT ACTATACACA 
TATCCTCCCC TCAGCCTTGC CCTAGGCCAT AATTGTTCAC AATGGGATGT CATCTCCAGA 
CATATAGGAT TGTCCAGGCT TCCCCTGGAT GAATATTTGA TTGTGTTGCG CCTGGTGTGT 
CCTGACCTGG GAGTTTTTGC CGTTTCCTTG GTAACAATCA TTGTCTGTAA CAGACTGGTC 
AAGAATAGAG AAACAGTTTC TGCTGCCAAC ATCACATCGT TAGAAGATAA TTCAGACTGC 
ACACTCACAG AGGATGAAGA TGATGTAAAT GATGATGATC ATCTCGAAGA AGAGGAGGAC 
ACAGCCAGTG ATAAAGATGA AGAGAGGACT TTACACTGGG ATTCTGAAGA AGAGGTTTCC 
ACAGTAACAA GAGCCAAGCA ACTGGCCGAG CAGTTCAAGG CCACGGCCTG GAAGGTTCTG 
TGGGACCTCA GGAGAATCTT GGCCATCAGC TTGTTGGCTT TGGCTGGTAT CACCATGCCT 
TCAGCCTTTA CAGCCATCTA TTTCTTGCTG TTTATATGTT TGTGCACGTG GTGGGCCTTC 
CACCTCCCCA TCAGCCATCT GGGATTCAAC GCCCTGTGTG TGATGTTGAG CTTCTTCACA 
TCTGGCCACA TGGTTTGTTT GTACTTGTAC CAGAGTGTGT TGGCCCAGGC ACTGTTCCCG 
CCCCCCTCTC TGTGGGCTAG ACTTTTTGGC CTGAAGGACA TCATCCAACC TGGAAACTGC 
TCCTCACCCT ATGACATGAC ACTCAATGTC AGTTATGAGT GGCCAGTTTA TGTCAGTCCG 
GGGATCCTCT TCCTGCTCTA CATAACCTTA GCAGCTGTCC TCAAGACGGA AAGCTATGGA 
AAAGCAAACC AGGAGCAAAC GCAGGAGGAA ACAGGCGCAT CAACGGAGGA CATGCTGGAG 
TTAAAGACAT GGAACAAACA CACAAAAAGG ACGACGACGA CTCAGAGTGC GTCCTTTTGT 
CCGTGGGAAC AGAGACATGC AGCAGCATCG AGGAAAAGCC TCCAGAGGGA CTCCTGCAGC 
CAGAGCTGGG AGGCTCCTGT CACCCACCCA GGGGAGAGCC CCTTCAGTCT GCTGGGGAGG 
GTGATCATGC AGCAGAGCTA CGTCTGTGCT CTCATCGCCA TGATGGTGTG GAGCATCATG 
TACCACAGCT GGCTGACTTT TGTGCTACTA CTGTGGGCTT GTCTCATCTG GATCCTGCGC 
ACAAGGCGTC AAGCCGCCAC CTTGTGTTCC CCGTTCATCC TGCTTTATGG TCTGGCTCTT 
TGCTGTCTGC AGTACATCTG GGCTATTGAG CTCCAGCCTG AATTACCCAC CACACTAGGA 
ACCATGAGAC TCAACCAGCT GGGACTAGAT AGAGCCAAGT ACCCCTGTCT CAGGCTGGGA 
GCTATGGTTC TCTTCACTCT GACATTCTGG CTGCTCGTAC GTCAGTCAGT GAAGGAGAAC 
TTCAGCAAGA AGAGAAAAGT GGTCACACCT CTAGAAGATG TCCGGACAGA GGAGAAAGGT 
GAGAAGGAAC CGGTGCTGAA GGTTGTGGGA GCCCTGGTGA TGAGCTGCTA TGCCAAATAT 
TGGATCTTTG TGTGCGGAGG AATGTTCATC ATGGTGTCGT TTGCTGGGAA ACTTGTTGGA 
TACAAGATTG TCTACATGCT GATGTTCTTG ATCTGCCTCT GTCTGTATCA GGTCTGTTAC 
TCTCTATGGA GGCGTCTGTT GAAGCTTTTC TGGTGGCTTG TCGTGGCCTA CACCATGCTG 
GTGCTCATCT CCATCTACAC GTACCAGTTT GAAGACTTCC CTGGATACTG GAGAAACTTT 
ACTGGTTTCA CGGAACAACA GTTAGAAGCT ATTGGTTTGG AGACGTTTGC TCTATCGGAA 
CTCTTCACCA GCATTCTGAT TCCGGGCTTC TTCCTGCTGG CCTGTATTCT GCAGCTGCAC 
TACTTCCACA AACCCTTCAT GCGAATCACC GACCTAGAGG ACGTTATGCC GTTACCAAGG 
AAGGAGAAGA AGTTTTTAAG GACGGAGCTT CCTCACTCCG ATGATGAAGA GGAGGAACTG 
GTTGTTAGCA ATGAGGATTC TGATGATGAA GTGGAGCTCA CGCCCAGTAA GTGGGGTCTG 
GTAATGGACA GGTTACTGGT TCTGTCCAGA CGGTTCTCTG ACACTCTCAC TAAAGTTCAA 
GAGTTTGTCT GGAGGCTTCT GGAGCTCCAC ATTGCAAAGA TGGTGGCCCT GTTCGCTGTG 
TGGGTGGCCC TTGAAGAGCC ATCATTGATG AACCTGGTCC TGGTGGTCTT GTGGACCCTG 
GCGATGCCCT ACAGCGCCTT CAGGCCCATG ACTTCCTGTC TGTCCACAGT CTGGGTCTGT 
GTCATCATCG TGTGTAAAAT GCTGTACCAG CTCAGTGTTG TCAACCCCGT AGAGTACTCC 
AGTAACTGCA CCAAGCCTTT ATCAAATGAA ACGAACCTTT CACCAGAGGA GATATTAGAC 
TCTTCTCTAT ATAAAGAGCC GGTGGACCCT GCGAGATGGT TCGGTATCAG GAAGGACGCC 
ACCATCCTGG GATACAGCCA GAGTCACCTG TTATTGCTGA TGATGCTGGT GTTCGAGGCT 
ACAGTCTACC GTCACCAAGC TCACCACTAC CGCCAGCTCC AAAAGTCTCC TCCTACCATC 
CCAACTCTGT TTGCCACCGC CACAAGGGAC ACTCTGGACC AGGGCCTCAT CCCCTGCCTC 
AAGTACCTGC TTAACTACAC CTTTTACAAG TTTGGATTAG AGATTTGTTT CCTGATGATG 
GTGAATGTGA TTGGCCAGAG GATGAACTTC TTAATGATAA TCCATGGCTG TTGGCTGGTG 
GTCATCTTGT CGAAGCGCCG GCGGGCAGCT ATTGCCAGGG TCTGGCCCAG CTATTGTGTC 
TTCCTGTCTG TCTTCATGAT CTACCAGTAT CTGCTGTGTT TAGGCATACC GCCCGCTCTG 
TGCATAGAGT ACCCATGGAG ATGGAAGACT CCAGTCCTGA TCAACTCAGC TCTTATAAAA 
TGGATCTATC TGCCCGACTT TTACACAGTA CCTAACTCCA GAAACCTCAT CGCGGATTTT 
GTGTTGCTGA TGTGTGCGTC CCAGCAGTGG AAGGTGTTTG AGTGTGAGCG CACAAGGGAG 
TGGACGGTTC TGGCAGGAGA GAACGTAGAT GACCCAGAAC ACGTGGACGG TCGACTCTGT 
AATCCGGCAG CAAATTTCAT CAACTGCAGG AGTTACCTGG ACATGGCTAA AGTTCTGGTG 
TTTCGACACT TGTTCTGGTT TGTGCTGTCA GTGGTCTTCA TTACTGGAGC AACCAGAATC 
TCGGTGTTTG GGCTTGGATA CCTCCTGGCC TGTTTTTTTT TCCTTCTGTT CGGGACTCAT 
TTCTTGGTCA AGCCCTGCAG GACCCGCCTC AACCTGTGGG ACTGTCTCAT CATCTATAAT 
GTGGCAGTCA TTATATCCAA AAATATGTTA TCAATTCTGG CGTGCGTGTT TGTTTCTGCG 
ATGCAGAATG GCTTCTGTTG GGTGATTCAA CTCTTCAGCC TGGTTTGCAC AGTCAATGGA 
TACTATGACC CAAAAGCAGT GAATGACAAG AACTGCAGTC TCCCAGTGGG AGAGGCCGGG 
ATTCTCTGGG ACAGTATCTG CTTCTTCTTC CTCTTGCTTC AGAGGAGGGT CTTTCTCAGT 
TTCTACTTCC TACATGTTGT CGCTGAGCTA CAGGCTTCTG CTAAACAAGC CTCCAGGGGG 
TTTGAACTCT TCAAAGCCAG TATTGTGAAG AACACGAATA TCTACCAACA AGCTGAGGAG 
AAATCGCTGT CTCAGCTCAA AAGATCAATG CAGAGAATTC GTTGCAGACA GAAGAAGTAC 
AGAGCTGGAC TGAGAGGAAG CGACGAGTCC TCAGAACCCG ACACTGCTGG TTGCCCGGGT 
CACTCCACAC CTGAAGCAGA ACTTGTTGAC ACAGGTGACA TTGGAGCCGA CCCCCACATG 
GACAAGAAGT TGAGGAAGAA TAAGGGGTAT CACCCCTGGT GGGACCATGC CACAGCCCGC 
CACGCAGGAG ATTATTACCT TTTTGAATCC GAAAGTGAAG ATGAAGAAGA GTTACAGTCT 
GAAGAACAGA AGCCTCCAAA ACAGACGGCA TGTCAGCTAG CGTACCAAAC ATGGGTGAGC 
AGCGTGAAAA CAGCTCTCAG AGAACGTCAG AGTCTGCAAA GACATCTAAG GACTAAGAAA 
CCAGAAAAGG ACTCACAAAA CACAACAGGC TGTGGGAACA GTGATGGGTT TGAGGATTCT 
GATAATCCGG AGGAGGAAAA GCCATCTGCC CACGGTGAGA TGGTCCAGAG GATCTTAGAT 
CTTCTGCATT TTTTGTGGGC CATCGTTTTG GCCATGGTCG ATGGGATGAC TCTGTGGTTA 
AACCTGCTGA CCAAACAGTA CCGAGAAATG TCTGTCGTTC TGTGCAATGA ACGCTACTTC 
ATAACACACA AGATACAGCA GCATCACACA ACAGAGTCAA CCAATGGGAA GTTATCTGAA 
GACAGGGACA GTTCCAGGCT GGAGCTGGTC TTTGGTGAAG CTGAAGCAGC AAAATCAGCA 
GGATACAGCT GCTTGGAGGT ATGTAGTGGG GAGTTGACAG AGCAGTCGCC CCCCACTGCA 
AGTGCCATCA GCAGGAGTCC AGAAGCCCAG AGCAGCAGCA TGGAGCTGCT GGTCTGCCCA 
TCAAAACAGC AAAGACACAA CAGAACAGCC AGCGAGCTCC TGGACGACAG AGAGTCCTTC 
ATTGAGGAGC TGGAAGACAG CAGATCATTC TTCAAAAGCC AGAATCGTCT GCTGAAGCTG 
CTGTTTGCCC TGTACAACCT GCTGGCTGCC AACTCAGAAG TAGCCTGTTA CTTAATCATC 
GTGCTGAACA ACATGGTCAC TGCCTCGGTC ATCTCCCTCA TTCTGCCCAT CTTGGTTTTC 
CTTTGGGCGA TGTTGTCTGT GCCGAGGCCA ACCAAGAGAT TTTGGATGAC AGCTATTGTC 
TACACAGAGG TTATGGTGGT TGTAAAATAC TTTTTCCAGT TTGGATTCTT TCCTTGGAAC 
AGTGCCTATG AAATGGCAGT GAATGATGAT AAACCGTTCT TCCCACCTCG TATCCTGGGC 
CTCGAGAAGA CCGACAACTA CATCAGATAT GATCTTCTTC AGCTACTGGC ACTGTTCTTC 
CACAGGTCCC TGCTCAAGCG TTATGGCCTG TGGGACCACG AGGGACCCTT AGAGGAGCAG 
AGTCCTACAC CAGACAACGT GGAGTCAGCT GATTCAAATG TCAGCGCTGC TGCTGAGATT 
CCACAATCGG CTCCAAAGGA ACCAATAAAC ACCGGGTCAG AAGATGGACA GGAACCTCCA 
TCGGTGCCAG AGGACATGTT GGAATCTGAT GGTAGTTGGC AGGAAGCAAG AACGGAAGAA 
GACCCCAAAC AAGAAGTGAA AAGCACTTTT ATCCGCTTTC GCAAGAAAAT TCAACATCCC 
AAAGGACCAC GCAGCAACGC GCCCCCTGCT GACACCACTG ACGCCACTAA AGCACCAACA 
AGAAAGAGAA AGAAAGTGAC GAAGGGAGAA ACGGGAGGTC AGAGATTGGT GGCGACAGTA 
CAAAAGATCA AACACGTCTT CGTCTCCGGG ATTCAGAGAG TTTACCGTCC AGCCAAATGT 
TTCTTTGGGA GCATCCTCCG TGCTGAGTAT CGGGCTCCCA CTGACGTCTA CGCACTCATG 
TTCCTCACTG ACGTCGTCGA CTTCATCGTC ATCATCTTTG GATTTTGGGC TTTTGGGAAA 
CACAGCGCCG CCGCTGATAT CGCCTCCACG CTGTCGGACG ACCAGGTCCC GGAGGCTTTT 
CTGGCGATGC TGCTCATCCA GTTCAGCACC ATGATCATCG ACAGAGCGTT GTACCTGCGC 
AAGGCTGTCT TAGGAAAGCT CATCTTCCAG GTGATCCTGG TGTTTGGGAT CCACCTGTGG 
ATGTTCTTTA TCCTGCCGGC TGTCACAGAA AGGATGTTCA ATCAGAACTT TGTCGCTCAG 
CTCTGGTACT TTGTCAAGTG CATTTACTTC GGCCTGTCGG CCCTCCAGAT CCGCTGTGGA 
TACCCGACTC GTATCCTGGG AAACTTCCTC ACCAAGAATT ACAACCACCT CAACCTGTTC 
CTTTTCCAAG GGTTTCGGCT GGTGCCCTTC CTGGTGGAGC TGCGGGCTGT GATGGACTGG 
GTCTGGACTG ACACCACTCT GTCTCTGTCA AACTGGATGT GTGTGGAGGA CATATACGCC 
AACATTTTTA TCATTAAATG CAGCCGTGAA ACAGAGAAGA AATACCCACA GCCTAAAGGA 
CAGAAGAAGA AGAAAATAGT CAAATATGGG ATGGGAGGAC TGATCATTTT CTTCTTGATC 
TGCATCATTT GGTTTCCTCT TCTTTTCATT TCGCTGGTCA GATCGGTGGT GGGCGTGGTC 
AACCACCCCA TCGACGTGAC CGTCACCATC AAGCTGGGAG GGTACGAGCC CCTCTTCACC 
ATGAGTGTGC AGCAACAGTC CATCCAGCCC TTTACAATGG TTGAATATGA ACAACTCACA 
AAGAAGTTTG GGAACAATGC GGCGGCCATG CAGTTTATAA CTCTGTATAA TTATGAGGAC 
ATTGTGACGG CCATGATTGA AGGTAGTTCT GGATCAGTGT GGAGGATCAG CCCCCCGAGC 
AGACAGGAAG TCATAAAGGA ACTGCTGAGT AGCCGTGCTG ACCTCACGCT GCGCCTGGCC 
TGGAATTTCC AAAGGGACCT GGGCAAGGGA GGGACGGTTG AACACTCCTT TGACAAGCAC 
TCAGTCCCTC TGGAGCCAGG GAACCCCGTC AGGGCTGATA TGGCCTCGTT GCTGCTCGGC 
AATCGTACCG AGCCGGTCCA CGTCCCTAAT TTGTTCCCGA ACTATCTACG TGCCCCCAAT 
GGAGCTGAAG CCAAACCTGT CAGTCAGCTA TTTAAAGGTC AGAAATACAT TGAAAATGGA 
TGGATTATTG ACAGGTCCCT GAACGGGGGG GTGAACCAGG AGTGGTGGGA TATTGCCATT 
GAAGGTTGTG CCCCCTCCTC CTGTGGGGTT CTGCCCATGG TCATATTTAA TGACAAAGTC 
AGTCCACCAA GTCTGGGCTT CCTTGCTGGA TATGGGATCA TGGGTTTGTA TGTTTCCGTG 
GTCTTAGTCA TCGGGAAATT TGTGCGTGGC TTCTTCAGTG AAATCTCACA CTCCATTATG 
TTTGAGGAGT TGCCCTGTGT GGACCGAATC CTGAAGCTGT GCACCGATAT CTTCCTGGTT 
CGTGAGACAG GAGAGCTGGA ACTAGAAGAG GAACTTTACT CTAAGCTGAT CTTCCTCTAT 
CGCTCTCCAG AGACGATGAT CAAATGGACC AGGGACATTA ATGCCCAAGA CCAGAATCCA 
GGAATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101071632 ( XM_029832472.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029688332.1 Takifugu rubripes piezo-type mechanosensitive ion channel component 1 (LOC101071632), mRNA.

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CloneID	OTo40515
Clone ID Related Accession (Same CDS sequence)	XM_029832472.1
Accession Version	XM_029832472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	piezo-type mechanosensitive ion channel component 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021821654.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTCC AGGTGATCGT GTGGCTCTTC TACTACCTTC TGGTGCCCAC GGTGCTGTTA 
ACAGCCTGCC TTTTTCGCTA CAACCTCCTG TCTCTGATCT ACTTTGTCTA CCTACTGCTG 
CTGCCATGGT TCCAGTGTCC CAATAAACAC ACAATCAAAG GTCACACAGG AAACTTTATT 
CGAGCCGTAT TTTTTACCAG TCTGGTGTTT GTCCTGGCAC ATATATGTTT CCAGACGGTA 
CTATACACAT ATCCTCCCCT CAGCCTTGCC CTAGGCCATA ATTGTTCACA ATGGGATGTC 
ATCTCCAGAC ATATAGGATT GTCCAGGCTT CCCCTGGATG AATATTTGAT TGTGTTGCGC 
CTGGTGTGTC CTGACCTGGG AGTTTTTGCC GTTTCCTTGG TAACAATCAT TGTCTGTAAC 
AGACTGGTCA AGAATAGAGA AACAGTTTCT GCTGCCAACA TCACATCGTT AGAAGATAAT 
TCAGACTGCA CACTCACAGA GGATGAAGAT GATGTAAATG ATGATGATCA TCTCGAAGAA 
GAGGAGGACA CAGCCAGTGA TAAAGATGAA GAGAGGACTT TACACTGGGA TTCTGAAGAA 
GAGGTTTCCA CAGTAACAAG AGCCAAGCAA CTGGCCGAGC AGTTCAAGGC CACGGCCTGG 
AAGGTTCTGT GGGACCTCAG GAGAATCTTG GCCATCAGCT TGTTGGCTTT GGCTGGTATC 
ACCATGCCTT CAGCCTTTAC AGCCATCTAT TTCTTGCTGT TTATATGTTT GTGCACGTGG 
TGGGCCTTCC ACCTCCCCAT CAGCCATCTG GGATTCAACG CCCTGTGTGT GATGTTGAGC 
TTCTTCACAT CTGGCCACAT GGTTTGTTTG TACTTGTACC AGAGTGTGTT GGCCCAGGCA 
CTGTTCCCGC CCCCCTCTCT GTGGGCTAGA CTTTTTGGCC TGAAGGACAT CATCCAACCT 
GGAAACTGCT CCTCACCCTA TGACATGACA CTCAATGTCA GTTATGAGTG GCCAGTTTAT 
GTCAGTCCGG GGATCCTCTT CCTGCTCTAC ATAACCTTAG CAGCTGTCCT CAAGACGGAA 
AGCTATGGAA AAGCAAACCA GGAGCAAACG CAGGAGGAAA CAGGCGCATC AACGGAGGAC 
ATGCTGGAGT TAAAGACATG GAACAAACAC ACAAAAAGGA CCACGGACGA CTCAGAGTGC 
GTCCTTTTGT CCGTGGGAAC AGAGACATGC AGCAGCATCG AGGAAAAGCC TCCAGAGGGA 
CTCCTGCAGC CAGAGCTGGG AGGCTCCTGT CACCCACCCA GGGAGAGCCC CTTCAGTCTG 
CTGGGGAGGG TGATCATGCA GCAGAGCTAC GTCTGTGCTC TCATCGCCAT GATGGTGTGG 
AGCATCATGT ACCACAGCTG GCTGACTTTT GTGCTACTAC TGTGGGCTTG TCTCATCTGG 
ATCCTGCGCA CAAGGCGTCA AGCCGCCACC TTGTGTTCCC CGTTCATCCT GCTTTATGGT 
CTGGCTCTTT GCTGTCTGCA GTACATCTGG GCTATTGAGC TCCAGCCTGA ATTACCCACC 
ACACTAGGAA CCATGAGACT CAACCAGCTG GGACTAGATA GAGCCAAGTA CCCCTGTCTC 
AGGCTGGGAG CTATGGTTCT CTTCACTCTG ACATTCTGGC TGCTCGTACG TCAGTCAGTG 
AAGGAGAACT TCAGCAAGAA GAGAAAAGTG GTCACACCTC TAGAAGATGT CCGGACAGAG 
GAGAAAGGTG AGAAGGAACC GGTGCTGAAG GTTGTGGGAG CCCTGGTGAT GAGCTGCTAT 
GCCAAATATT GGATCTTTGT GTGCGGAGGA ATGTTCATCA TGGTGTCGTT TGCTGGGAAA 
CTTGTTGGAT ACAAGATTGT CTACATGCTG ATGTTCTTGA TCTGCCTCTG TCTGTATCAG 
GTCTGTTACT CTCTATGGAG GCGTCTGTTG AAGCTTTTCT GGTGGCTTGT CGTGGCCTAC 
ACCATGCTGG TGCTCATCTC CATCTACACG TACCAGTTTG AAGACTTCCC TGGATACTGG 
AGAAACTTTA CTGGTTTCAC GGAACAACAG TTAGAAGCTA TTGGTTTGGA GACGTTTGCT 
CTATCGGAAC TCTTCACCAG CATTCTGATT CCGGGCTTCT TCCTGCTGGC CTGTATTCTG 
CAGCTGCACT ACTTCCACAA ACCCTTCATG CGAATCACCG ACCTAGAGGA CGTTACGCCG 
TTACCAAGGA AGGAGAAGAA GTTTTTAAGG ACGGAGCTTC CTCACTCCGA TGATGAAGAG 
GAGGAACTGG TTGTTAGCAA TGAGGATTCT GATGATGAAG TGGAGCTCAC GCCCAGTAAG 
TGGGGTCTGG TAATGGACAG GTTACTGGTT CTGTCCAGAC GGTTCTCTGA CACTCTCACT 
AAAGTTCAAG AGTTTGTCTG GAGGCTTCTG GAGCTCCACA TTGCAAAGAT GGTGGCCCTG 
TTCGCTGTGT GGGTGGCCCT TGAAGAGCCA TCATTGATGA ACCTGGTCCT GGTGGTCTTG 
TGGACCCTGG CGATGCCCTA CAGCGCCTTC AGGCCCATGA CTTCCTGTCT GTCCACAGTC 
TGGGTCTGTG TCATCATCGT GTGTAAAATG CTGTACCAGC TCAGTGTTGT CAACCCCGTA 
GAGTACTCCA GTAACTGCAC CAAGCCTTTA TCAAATGAAA CGAACCTTTC ACCAGAGGAG 
ATATTAGACT CTTCTCTATA TAAAGAGCCG GTGGACCCTG CGAGATGGTT CGGTATCAGG 
AAGGACGCCA CCATCCTGGG ATACAGCCAG AGTCACCTGT TATTGCTGAT GATGCTGGTG 
TTCGAGGCTA CAGTCTACCG TCACCAAGCT CACCACTACC GCCAGCTCCA AAAGTCTCCT 
CCTACCATCC CAACTCTGTT TGCCACCGCC ACAAGGGACA CTCTGGACCA GGGCCTCATC 
CCCTGCCTCA AGTACCTGCT TAACTACACC TTTTACAAGT TTGGATTAGA GATTTGTTTC 
CTGATGATGG TGAATGTGAT TGGCCAGAGG ATGAACTTCT TAATGATAAT CCACGGCTGT 
TGGCTGGTGG TCATCTTGTC GAAGCGCCGG CGGGCAGCTA TTGCCAGGGT CTGGCCCAGC 
TATTGTGTCT TCCTGTCTGT CTTCATGATC TACCAGTATC TGCTGTGTTT AGGCATACCA 
CCCGCTCTGT GCATAGAGTA CCCATGGAGA TGGAAGACTC CAGTCCTGAT CAACTCAGCT 
CTTATAAAAT GGATCTATCT GCCCGACTTT TACACAGTAC CTAACTCCAG AAACCTCATC 
GCGGATTTTG TGTTGCTGAT GTGTGCGTCC CAGCAGTGGA AGGTGTTTGA GTGTGAGCGC 
ACAAGGGAGT GGACGGTTCT GGCAGGAGAG AACGTAGATG ACCCAGAACA CGTGGACGGT 
CGACTCTGTA ATCCGGCAGC AAATTTCATC AACTGCAGGA GTTACCTGGA CATGGCTAAA 
GTTCTGGTGT TTCGACACTT GTTCTGGTTT GTGCTGTCAG TGGTCTTCAT TACTGGAGCA 
ACCAGAATCT CGGTGTTTGG GCTTGGATAC CTCCTGGCCT GTTTTTTTTT CCTTCTGTTC 
GGGACTCATT TCTTGGTCAA GCCCTGCAGG ACCCGTCTCA ACCTGTGGGA CTGTCTCATC 
ATCTATAATG TGGCAGTCAT TATATCCAAA AATATGTTAT CAATTCTGGC GTGCGTGTTT 
GTTTCTGCGA TGCAGAATGG CTTCTGTTGG GTGATTCAAC TCTTCAGCCT GGTTTGCACA 
GTCAATGGAT ACTATGACCC AAAAGCAGTG AATGACAAGA ACTGCAGTCT CCCAGTGGGA 
GAGGCCGGGA TTCTCTGGGA CAGTATCTGC TTCTTCTTCC TCTTGCTTCA GAGGAGGGTC 
TTTCTCAGTT TCTACTTCCT ACATGTTGTC GCTGAGCTAC AGGCTTCTGC TAAACAAGCC 
TCCAGGGGGT TTGAACTCTT CAAAGCCAGT ATTGTGAAGA ACACGAATAT CTACCAACAA 
GCTGAGGAGA AATCGCTGTC TCAGCTCAAA AGATCAATGC AGAGAATTCG TTGCAGACAG 
AAGAAGTACA GAGCTGGACT GAGAGGAAGC GACGAGTCCT CAGAACCCGA CACTGCTGGT 
TGCCCGGGTC ACTCCACACC TGAAGCAGAA CTTGTTGACA CAGACCCCCA CATGGACAAG 
AAGTTGAGGA AGAATAAGGG GTATCACCCC TGGTGGGACC ATGCCACAGC CCGCCACGCA 
GGAGATTATT ACCTTTTTGA ATCCGAAAGT GAAGATGAAG AAGAGTTACA GTCTGAAGAA 
CAGAAGCCTC CAAAACAGAC GGCATGTCAG CTAGCGTACC AAACATGGGT GAGCAGCGTG 
AAAACAGCTC TCAGAGAACG TCAGAGTCTG CAAAGACATC TAAGGACTAA GAAACCAGAA 
AAGGACTCAC AAAACACAAC AGGCTGTGGG AACAGTGATG GGTTTGAGGA TTCTGATAAT 
CCGGAGGAGG AAAAGCCATC TGCCCACGGT GAGATGGTCC AGAGGATCTT AGATCTTCTG 
CATTTTTTGT GGGCCATCGT TTTGGCCATG GTCGATGGGA TGACTCTGTG GTTAAACCTG 
CTGACCAAAC AGTACCGAGA AATGTCTGTC GTTCTGTGCA ATGAACGCTA CTTCATAACA 
CACAAGATAC AGCAGCATCA CACAACAGAG TCAACCAATG GGAAGTTATC TGAAGACAGG 
GACAGTTCCA GGCTGGAGCT GGTCTTTGGT GAAGCTGAAG CAGCAAAATC AGCAGGATAC 
AGCTGCTTGG AGGTATGTAG TGGGGAGTTG ACAGAGCAGT CGCCCCCCAC TGCAAGTGCC 
ATCAGCAGGA GTCCAGAAGC CCAGAGCAGC AGCATGGAGC TGCTGGTCTG CCCATCAAAA 
CAGCAAAGAC ACAACAGAAC AGCCAGCGAG CTCCTGGACG ACAGAGAGTC CTTCATTGAG 
GAGCTGGAAG ACAGCAGATC ATTCTTCAAA AGCCAGAATC GTCTGCTGAA GCTGCTGTTT 
GCCCTGTACA ACCTGCTGGC TGCCAACTCA GAAGTAGCCT GTTACTTAAT CATCGTGCTG 
AACAACATGG TCACTGCCTC GGTCATCTCC CTCATTCTGC CCATCTTGGT TTTCCTTTGG 
GCGATGTTGT CTGTGCCGAG GCCAACCAAG AGATTTTGGA TGACAGCTAT TGTCTACACA 
GAGGTTATGG TGGTTGTAAA ATACTTTTTC CAGTTTGGAT TCTTTCCTTG GAACAGTGCC 
TATGAAATGG CAGTGAATGA TGATAAACCG TTCTTCCCAC CTCGTATCCT GGGCCTCGAG 
AAGACCGACA ACTACATCAG ATATGATCTT CTTCAGCTAC TGGCACTGTT CTTCCACAGG 
TCCCTGCTCA AGCGTTATGG CCTGTGGGAC CACGAGGGAC CCTTAGAGGA GCAGAGTCCT 
ACACCAGACA ACGTGGAGTC AGCTGATTCA AATGTCAGCG CTGCTGCTGA GATTCCACAA 
TCGGCTCCAA AGGAACCAAT AACCACCGGG TCAGAAGATG GACAGGAACC TCCATCGGTG 
CCAGAGGACA TGTTGGAATC TGATGGTAGT TGGCAGGAAG CAAGAACGGA AGAAGACCCC 
AAACAAGAAG TGAAAAGCAC TTTTATCCGC TTTCGCAAGA AAATTCAACA TCCCAAAGGA 
CCACGCAGCA ACGCGCCCCC TGCTGACACC ACTGACGCCA CTAAAGCACC AACAAGAAAG 
AGAAAGAAAG TGACGAAGGG AGAAACGGGA GGTCAGAGAT TGGTGGCGAC AGTACAAAAG 
ATCAAACACG TCTTCGTCTC CGGGATTCAG AGAGTTTACC GTCCAGCCAA ATGTTTCTTT 
GGGAGCATCC TCCGTGCTGA GTATCGGGCT CCCACTGACG TCTACGCACT CATGTTCCTC 
ACTGACGTCG TCGACTTCAT CGTCATCATC TTTGGATTTT GGGCTTTTGG GAAACACAGC 
GCCGCCGCTG ATATCGCCTC CACGCTGTCG GACGACCAGG TCCCGGAGGC TTTTCTGGCG 
ATGCTGCTCA TCCAGTTCAG CACCATGATC ATCGACAGAG CGTTGTACCT GCGCAAGGCT 
GTCTTAGGAA AGCTCATCTT CCAGGTGATC CTGGTGTTTG GGATCCACCT GTGGATGTTC 
TTTATCCTGC CGGCTGTCAC AGAAAGGATG TTCAATCAGA ACTTTGTCGC TCAGCTCTGG 
TACTTTGTCA AGTGTATTTA CTTCGGCCTG TCGGCCCTCC AGATCCGCTG TGGATACCCG 
ACTCGTATCC TGGGAAACTT CCTCACCAAG AATTACAACC ACCTCAACCT GTTCCTTTTC 
CAAGGGTTTC GGCTGGTGCC CTTCCTGGTG GAGCTGCGGG CTGTGATGGA CTGGGTCTGG 
ACTGACACCA CTCTGTCTCT GTCAAACTGG ATGTGTGTGG AGGACATATA CGCCAACATT 
TTTATCATTA AATGCAGCCG TGAAACAGAG AAGAAATACC CACAGCCTAA AGGACAGAAG 
AAGAAGAAAA TAGTCAAATA TGGGATGGGA GGACTGATCA TTTTCTTCTT GATCTGCATC 
ATTTGGTTTC CTCTTCTTTT CATTTCGCTG GTCAGATCGG TGGTGGGCGT GGTCAACCAC 
CCCATCGACG TGACCGTCAC CATCAAGCTG GGAGGGTACG AGCCCCTCTT CACCATGAGT 
GTGCAGCAAC AGTCCATCCA GCCCTTTACA ATGGTTGAAT ATGAACAACT CACAAAGAAG 
TTTGGGAACA ATGCGGCGGC CATGCAGTTT ATAACTCTGT ATAATTATGA GGACATTGTG 
ACGGCCATGA TTGAAGGTAG TTCTGGATCA GTGTGGAGGA TCAGCCCCCC GAGCAGACAG 
GAAGTCATAA AGGAACTGCT GAGTAGCCGT GCTGACCTCA CGCTGCGCCT GGCCTGGAAT 
TTCCAAAGGG ACCTGGGCAA GGGAGGGACG GTTGAACACT CCTTTGACAA GCACTCAGTC 
CCTCTGGAGC CAGGGAACCC CGTCAGGGCT GATATGGCCT CGTTGCTGCT CGGCAATCGT 
ACCGAGCCGG TCCACGTCCC TAATTTGTTC CCGAACTATC TACGTGCCCC CAATGGAGCT 
GAAGCCAAAC CTGTCAGTCA GCTATTTAAA GGTGGTGAAG AAGATTTTCT GAATATAACC 
CTGTCCCTGA AGAGTGACAG GTCCCTGAAC GGGGGGGTGA ACCAGGAGTG GTGGGATATT 
GCCATTGAAG GTTGTGCCCC CTCCTCCTGT GGGGTTCTGC CCATGGTCAT ATTTAATGAC 
AAAGTCAGTC CACCAAGTCT GGGCTTCCTT GCTGGATATG GGATCATGGG TTTGTATGTT 
TCCGTGGTCT TAGTCATCGG GAAATTTGTG CGTGGCTTCT TCAGTGAAAT CTCACACTCC 
ATTATGTTTG AGGAGTTGCC CTGTGTGGAC CGAATCCTGA AGCTGTGCAC CGATATCTTC 
CTGGTTCGTG AGACAGGAGA GCTGGAACTA GAAGAGGAAC TTTACTCTAA GCTGATCTTC 
CTCTATCGCT CTCCAGAGAC GATGATCAAA TGGACCAGGG ACATTAATGC CCAAGACCAG 
AATCCAGGAA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688332.1
CDS	393..8027
Translation	MDLQVIVWLFYYLLVPTVLLTACLFRYNLLSLIYFVYLLLLPWFQCPNKHTIKGHTGNFIRAVFFTSLVFVLAHICFQTVLYTYPPLSLALGHNCSQWDVISRHIGLSRLPLDEYLIVLRLVCPDLGVFAVSLVTIIVCNRLVKNRETVSAANITSLEDNSDCTLTEDEDDVNDDDHLEEEEDTASDKDEERTLHWDSEEEVSTVTRAKQLAEQFKATAWKVLWDLRRILAISLLALAGITMPSAFTAIYFLLFICLCTWWAFHLPISHLGFNALCVMLSFFTSGHMVCLYLYQSVLAQALFPPPSLWARLFGLKDIIQPGNCSSPYDMTLNVSYEWPVYVSPGILFLLYITLAAVLKTESYGKANQEQTQEETGASTEDMLELKTWNKHTKRTTDDSECVLLSVGTETCSSIEEKPPEGLLQPELGGSCHPPRESPFSLLGRVIMQQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWILRTRRQAATLCSPFILLYGLALCCLQYIWAIELQPELPTTLGTMRLNQLGLDRAKYPCLRLGAMVLFTLTFWLLVRQSVKENFSKKRKVVTPLEDVRTEEKGEKEPVLKVVGALVMSCYAKYWIFVCGGMFIMVSFAGKLVGYKIVYMLMFLICLCLYQVCYSLWRRLLKLFWWLVVAYTMLVLISIYTYQFEDFPGYWRNFTGFTEQQLEAIGLETFALSELFTSILIPGFFLLACILQLHYFHKPFMRITDLEDVTPLPRKEKKFLRTELPHSDDEEEELVVSNEDSDDEVELTPSKWGLVMDRLLVLSRRFSDTLTKVQEFVWRLLELHIAKMVALFAVWVALEEPSLMNLVLVVLWTLAMPYSAFRPMTSCLSTVWVCVIIVCKMLYQLSVVNPVEYSSNCTKPLSNETNLSPEEILDSSLYKEPVDPARWFGIRKDATILGYSQSHLLLLMMLVFEATVYRHQAHHYRQLQKSPPTIPTLFATATRDTLDQGLIPCLKYLLNYTFYKFGLEICFLMMVNVIGQRMNFLMIIHGCWLVVILSKRRRAAIARVWPSYCVFLSVFMIYQYLLCLGIPPALCIEYPWRWKTPVLINSALIKWIYLPDFYTVPNSRNLIADFVLLMCASQQWKVFECERTREWTVLAGENVDDPEHVDGRLCNPAANFINCRSYLDMAKVLVFRHLFWFVLSVVFITGATRISVFGLGYLLACFFFLLFGTHFLVKPCRTRLNLWDCLIIYNVAVIISKNMLSILACVFVSAMQNGFCWVIQLFSLVCTVNGYYDPKAVNDKNCSLPVGEAGILWDSICFFFLLLQRRVFLSFYFLHVVAELQASAKQASRGFELFKASIVKNTNIYQQAEEKSLSQLKRSMQRIRCRQKKYRAGLRGSDESSEPDTAGCPGHSTPEAELVDTDPHMDKKLRKNKGYHPWWDHATARHAGDYYLFESESEDEEELQSEEQKPPKQTACQLAYQTWVSSVKTALRERQSLQRHLRTKKPEKDSQNTTGCGNSDGFEDSDNPEEEKPSAHGEMVQRILDLLHFLWAIVLAMVDGMTLWLNLLTKQYREMSVVLCNERYFITHKIQQHHTTESTNGKLSEDRDSSRLELVFGEAEAAKSAGYSCLEVCSGELTEQSPPTASAISRSPEAQSSSMELLVCPSKQQRHNRTASELLDDRESFIEELEDSRSFFKSQNRLLKLLFALYNLLAANSEVACYLIIVLNNMVTASVISLILPILVFLWAMLSVPRPTKRFWMTAIVYTEVMVVVKYFFQFGFFPWNSAYEMAVNDDKPFFPPRILGLEKTDNYIRYDLLQLLALFFHRSLLKRYGLWDHEGPLEEQSPTPDNVESADSNVSAAAEIPQSAPKEPITTGSEDGQEPPSVPEDMLESDGSWQEARTEEDPKQEVKSTFIRFRKKIQHPKGPRSNAPPADTTDATKAPTRKRKKVTKGETGGQRLVATVQKIKHVFVSGIQRVYRPAKCFFGSILRAEYRAPTDVYALMFLTDVVDFIVIIFGFWAFGKHSAAADIASTLSDDQVPEAFLAMLLIQFSTMIIDRALYLRKAVLGKLIFQVILVFGIHLWMFFILPAVTERMFNQNFVAQLWYFVKCIYFGLSALQIRCGYPTRILGNFLTKNYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIIFFLICIIWFPLLFISLVRSVVGVVNHPIDVTVTIKLGGYEPLFTMSVQQQSIQPFTMVEYEQLTKKFGNNAAAMQFITLYNYEDIVTAMIEGSSGSVWRISPPSRQEVIKELLSSRADLTLRLAWNFQRDLGKGGTVEHSFDKHSVPLEPGNPVRADMASLLLGNRTEPVHVPNLFPNYLRAPNGAEAKPVSQLFKGGEEDFLNITLSLKSDRSLNGGVNQEWWDIAIEGCAPSSCGVLPMVIFNDKVSPPSLGFLAGYGIMGLYVSVVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCTDIFLVRETGELELEEELYSKLIFLYRSPETMIKWTRDINAQDQNPGI

Target ORF information:

RefSeq Version	XM_029832472.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes piezo-type mechanosensitive ion channel component 1 (LOC101071632), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832472.1

ORF Insert Sequence:

ATGGATCTCC AGGTGATCGT GTGGCTCTTC TACTACCTTC TGGTGCCCAC GGTGCTGTTA 
ACAGCCTGCC TTTTTCGCTA CAACCTCCTG TCTCTGATCT ACTTTGTCTA CCTACTGCTG 
CTGCCATGGT TCCAGTGTCC CAATAAACAC ACAATCAAAG GTCACACAGG AAACTTTATT 
CGAGCCGTAT TTTTTACCAG TCTGGTGTTT GTCCTGGCAC ATATATGTTT CCAGACGGTA 
CTATACACAT ATCCTCCCCT CAGCCTTGCC CTAGGCCATA ATTGTTCACA ATGGGATGTC 
ATCTCCAGAC ATATAGGATT GTCCAGGCTT CCCCTGGATG AATATTTGAT TGTGTTGCGC 
CTGGTGTGTC CTGACCTGGG AGTTTTTGCC GTTTCCTTGG TAACAATCAT TGTCTGTAAC 
AGACTGGTCA AGAATAGAGA AACAGTTTCT GCTGCCAACA TCACATCGTT AGAAGATAAT 
TCAGACTGCA CACTCACAGA GGATGAAGAT GATGTAAATG ATGATGATCA TCTCGAAGAA 
GAGGAGGACA CAGCCAGTGA TAAAGATGAA GAGAGGACTT TACACTGGGA TTCTGAAGAA 
GAGGTTTCCA CAGTAACAAG AGCCAAGCAA CTGGCCGAGC AGTTCAAGGC CACGGCCTGG 
AAGGTTCTGT GGGACCTCAG GAGAATCTTG GCCATCAGCT TGTTGGCTTT GGCTGGTATC 
ACCATGCCTT CAGCCTTTAC AGCCATCTAT TTCTTGCTGT TTATATGTTT GTGCACGTGG 
TGGGCCTTCC ACCTCCCCAT CAGCCATCTG GGATTCAACG CCCTGTGTGT GATGTTGAGC 
TTCTTCACAT CTGGCCACAT GGTTTGTTTG TACTTGTACC AGAGTGTGTT GGCCCAGGCA 
CTGTTCCCGC CCCCCTCTCT GTGGGCTAGA CTTTTTGGCC TGAAGGACAT CATCCAACCT 
GGAAACTGCT CCTCACCCTA TGACATGACA CTCAATGTCA GTTATGAGTG GCCAGTTTAT 
GTCAGTCCGG GGATCCTCTT CCTGCTCTAC ATAACCTTAG CAGCTGTCCT CAAGACGGAA 
AGCTATGGAA AAGCAAACCA GGAGCAAACG CAGGAGGAAA CAGGCGCATC AACGGAGGAC 
ATGCTGGAGT TAAAGACATG GAACAAACAC ACAAAAAGGA CCACGGACGA CTCAGAGTGC 
GTCCTTTTGT CCGTGGGAAC AGAGACATGC AGCAGCATCG AGGAAAAGCC TCCAGAGGGA 
CTCCTGCAGC CAGAGCTGGG AGGCTCCTGT CACCCACCCA GGGAGAGCCC CTTCAGTCTG 
CTGGGGAGGG TGATCATGCA GCAGAGCTAC GTCTGTGCTC TCATCGCCAT GATGGTGTGG 
AGCATCATGT ACCACAGCTG GCTGACTTTT GTGCTACTAC TGTGGGCTTG TCTCATCTGG 
ATCCTGCGCA CAAGGCGTCA AGCCGCCACC TTGTGTTCCC CGTTCATCCT GCTTTATGGT 
CTGGCTCTTT GCTGTCTGCA GTACATCTGG GCTATTGAGC TCCAGCCTGA ATTACCCACC 
ACACTAGGAA CCATGAGACT CAACCAGCTG GGACTAGATA GAGCCAAGTA CCCCTGTCTC 
AGGCTGGGAG CTATGGTTCT CTTCACTCTG ACATTCTGGC TGCTCGTACG TCAGTCAGTG 
AAGGAGAACT TCAGCAAGAA GAGAAAAGTG GTCACACCTC TAGAAGATGT CCGGACAGAG 
GAGAAAGGTG AGAAGGAACC GGTGCTGAAG GTTGTGGGAG CCCTGGTGAT GAGCTGCTAT 
GCCAAATATT GGATCTTTGT GTGCGGAGGA ATGTTCATCA TGGTGTCGTT TGCTGGGAAA 
CTTGTTGGAT ACAAGATTGT CTACATGCTG ATGTTCTTGA TCTGCCTCTG TCTGTATCAG 
GTCTGTTACT CTCTATGGAG GCGTCTGTTG AAGCTTTTCT GGTGGCTTGT CGTGGCCTAC 
ACCATGCTGG TGCTCATCTC CATCTACACG TACCAGTTTG AAGACTTCCC TGGATACTGG 
AGAAACTTTA CTGGTTTCAC GGAACAACAG TTAGAAGCTA TTGGTTTGGA GACGTTTGCT 
CTATCGGAAC TCTTCACCAG CATTCTGATT CCGGGCTTCT TCCTGCTGGC CTGTATTCTG 
CAGCTGCACT ACTTCCACAA ACCCTTCATG CGAATCACCG ACCTAGAGGA CGTTACGCCG 
TTACCAAGGA AGGAGAAGAA GTTTTTAAGG ACGGAGCTTC CTCACTCCGA TGATGAAGAG 
GAGGAACTGG TTGTTAGCAA TGAGGATTCT GATGATGAAG TGGAGCTCAC GCCCAGTAAG 
TGGGGTCTGG TAATGGACAG GTTACTGGTT CTGTCCAGAC GGTTCTCTGA CACTCTCACT 
AAAGTTCAAG AGTTTGTCTG GAGGCTTCTG GAGCTCCACA TTGCAAAGAT GGTGGCCCTG 
TTCGCTGTGT GGGTGGCCCT TGAAGAGCCA TCATTGATGA ACCTGGTCCT GGTGGTCTTG 
TGGACCCTGG CGATGCCCTA CAGCGCCTTC AGGCCCATGA CTTCCTGTCT GTCCACAGTC 
TGGGTCTGTG TCATCATCGT GTGTAAAATG CTGTACCAGC TCAGTGTTGT CAACCCCGTA 
GAGTACTCCA GTAACTGCAC CAAGCCTTTA TCAAATGAAA CGAACCTTTC ACCAGAGGAG 
ATATTAGACT CTTCTCTATA TAAAGAGCCG GTGGACCCTG CGAGATGGTT CGGTATCAGG 
AAGGACGCCA CCATCCTGGG ATACAGCCAG AGTCACCTGT TATTGCTGAT GATGCTGGTG 
TTCGAGGCTA CAGTCTACCG TCACCAAGCT CACCACTACC GCCAGCTCCA AAAGTCTCCT 
CCTACCATCC CAACTCTGTT TGCCACCGCC ACAAGGGACA CTCTGGACCA GGGCCTCATC 
CCCTGCCTCA AGTACCTGCT TAACTACACC TTTTACAAGT TTGGATTAGA GATTTGTTTC 
CTGATGATGG TGAATGTGAT TGGCCAGAGG ATGAACTTCT TAATGATAAT CCACGGCTGT 
TGGCTGGTGG TCATCTTGTC GAAGCGCCGG CGGGCAGCTA TTGCCAGGGT CTGGCCCAGC 
TATTGTGTCT TCCTGTCTGT CTTCATGATC TACCAGTATC TGCTGTGTTT AGGCATACCA 
CCCGCTCTGT GCATAGAGTA CCCATGGAGA TGGAAGACTC CAGTCCTGAT CAACTCAGCT 
CTTATAAAAT GGATCTATCT GCCCGACTTT TACACAGTAC CTAACTCCAG AAACCTCATC 
GCGGATTTTG TGTTGCTGAT GTGTGCGTCC CAGCAGTGGA AGGTGTTTGA GTGTGAGCGC 
ACAAGGGAGT GGACGGTTCT GGCAGGAGAG AACGTAGATG ACCCAGAACA CGTGGACGGT 
CGACTCTGTA ATCCGGCAGC AAATTTCATC AACTGCAGGA GTTACCTGGA CATGGCTAAA 
GTTCTGGTGT TTCGACACTT GTTCTGGTTT GTGCTGTCAG TGGTCTTCAT TACTGGAGCA 
ACCAGAATCT CGGTGTTTGG GCTTGGATAC CTCCTGGCCT GTTTTTTTTT CCTTCTGTTC 
GGGACTCATT TCTTGGTCAA GCCCTGCAGG ACCCGTCTCA ACCTGTGGGA CTGTCTCATC 
ATCTATAATG TGGCAGTCAT TATATCCAAA AATATGTTAT CAATTCTGGC GTGCGTGTTT 
GTTTCTGCGA TGCAGAATGG CTTCTGTTGG GTGATTCAAC TCTTCAGCCT GGTTTGCACA 
GTCAATGGAT ACTATGACCC AAAAGCAGTG AATGACAAGA ACTGCAGTCT CCCAGTGGGA 
GAGGCCGGGA TTCTCTGGGA CAGTATCTGC TTCTTCTTCC TCTTGCTTCA GAGGAGGGTC 
TTTCTCAGTT TCTACTTCCT ACATGTTGTC GCTGAGCTAC AGGCTTCTGC TAAACAAGCC 
TCCAGGGGGT TTGAACTCTT CAAAGCCAGT ATTGTGAAGA ACACGAATAT CTACCAACAA 
GCTGAGGAGA AATCGCTGTC TCAGCTCAAA AGATCAATGC AGAGAATTCG TTGCAGACAG 
AAGAAGTACA GAGCTGGACT GAGAGGAAGC GACGAGTCCT CAGAACCCGA CACTGCTGGT 
TGCCCGGGTC ACTCCACACC TGAAGCAGAA CTTGTTGACA CAGACCCCCA CATGGACAAG 
AAGTTGAGGA AGAATAAGGG GTATCACCCC TGGTGGGACC ATGCCACAGC CCGCCACGCA 
GGAGATTATT ACCTTTTTGA ATCCGAAAGT GAAGATGAAG AAGAGTTACA GTCTGAAGAA 
CAGAAGCCTC CAAAACAGAC GGCATGTCAG CTAGCGTACC AAACATGGGT GAGCAGCGTG 
AAAACAGCTC TCAGAGAACG TCAGAGTCTG CAAAGACATC TAAGGACTAA GAAACCAGAA 
AAGGACTCAC AAAACACAAC AGGCTGTGGG AACAGTGATG GGTTTGAGGA TTCTGATAAT 
CCGGAGGAGG AAAAGCCATC TGCCCACGGT GAGATGGTCC AGAGGATCTT AGATCTTCTG 
CATTTTTTGT GGGCCATCGT TTTGGCCATG GTCGATGGGA TGACTCTGTG GTTAAACCTG 
CTGACCAAAC AGTACCGAGA AATGTCTGTC GTTCTGTGCA ATGAACGCTA CTTCATAACA 
CACAAGATAC AGCAGCATCA CACAACAGAG TCAACCAATG GGAAGTTATC TGAAGACAGG 
GACAGTTCCA GGCTGGAGCT GGTCTTTGGT GAAGCTGAAG CAGCAAAATC AGCAGGATAC 
AGCTGCTTGG AGGTATGTAG TGGGGAGTTG ACAGAGCAGT CGCCCCCCAC TGCAAGTGCC 
ATCAGCAGGA GTCCAGAAGC CCAGAGCAGC AGCATGGAGC TGCTGGTCTG CCCATCAAAA 
CAGCAAAGAC ACAACAGAAC AGCCAGCGAG CTCCTGGACG ACAGAGAGTC CTTCATTGAG 
GAGCTGGAAG ACAGCAGATC ATTCTTCAAA AGCCAGAATC GTCTGCTGAA GCTGCTGTTT 
GCCCTGTACA ACCTGCTGGC TGCCAACTCA GAAGTAGCCT GTTACTTAAT CATCGTGCTG 
AACAACATGG TCACTGCCTC GGTCATCTCC CTCATTCTGC CCATCTTGGT TTTCCTTTGG 
GCGATGTTGT CTGTGCCGAG GCCAACCAAG AGATTTTGGA TGACAGCTAT TGTCTACACA 
GAGGTTATGG TGGTTGTAAA ATACTTTTTC CAGTTTGGAT TCTTTCCTTG GAACAGTGCC 
TATGAAATGG CAGTGAATGA TGATAAACCG TTCTTCCCAC CTCGTATCCT GGGCCTCGAG 
AAGACCGACA ACTACATCAG ATATGATCTT CTTCAGCTAC TGGCACTGTT CTTCCACAGG 
TCCCTGCTCA AGCGTTATGG CCTGTGGGAC CACGAGGGAC CCTTAGAGGA GCAGAGTCCT 
ACACCAGACA ACGTGGAGTC AGCTGATTCA AATGTCAGCG CTGCTGCTGA GATTCCACAA 
TCGGCTCCAA AGGAACCAAT AACCACCGGG TCAGAAGATG GACAGGAACC TCCATCGGTG 
CCAGAGGACA TGTTGGAATC TGATGGTAGT TGGCAGGAAG CAAGAACGGA AGAAGACCCC 
AAACAAGAAG TGAAAAGCAC TTTTATCCGC TTTCGCAAGA AAATTCAACA TCCCAAAGGA 
CCACGCAGCA ACGCGCCCCC TGCTGACACC ACTGACGCCA CTAAAGCACC AACAAGAAAG 
AGAAAGAAAG TGACGAAGGG AGAAACGGGA GGTCAGAGAT TGGTGGCGAC AGTACAAAAG 
ATCAAACACG TCTTCGTCTC CGGGATTCAG AGAGTTTACC GTCCAGCCAA ATGTTTCTTT 
GGGAGCATCC TCCGTGCTGA GTATCGGGCT CCCACTGACG TCTACGCACT CATGTTCCTC 
ACTGACGTCG TCGACTTCAT CGTCATCATC TTTGGATTTT GGGCTTTTGG GAAACACAGC 
GCCGCCGCTG ATATCGCCTC CACGCTGTCG GACGACCAGG TCCCGGAGGC TTTTCTGGCG 
ATGCTGCTCA TCCAGTTCAG CACCATGATC ATCGACAGAG CGTTGTACCT GCGCAAGGCT 
GTCTTAGGAA AGCTCATCTT CCAGGTGATC CTGGTGTTTG GGATCCACCT GTGGATGTTC 
TTTATCCTGC CGGCTGTCAC AGAAAGGATG TTCAATCAGA ACTTTGTCGC TCAGCTCTGG 
TACTTTGTCA AGTGTATTTA CTTCGGCCTG TCGGCCCTCC AGATCCGCTG TGGATACCCG 
ACTCGTATCC TGGGAAACTT CCTCACCAAG AATTACAACC ACCTCAACCT GTTCCTTTTC 
CAAGGGTTTC GGCTGGTGCC CTTCCTGGTG GAGCTGCGGG CTGTGATGGA CTGGGTCTGG 
ACTGACACCA CTCTGTCTCT GTCAAACTGG ATGTGTGTGG AGGACATATA CGCCAACATT 
TTTATCATTA AATGCAGCCG TGAAACAGAG AAGAAATACC CACAGCCTAA AGGACAGAAG 
AAGAAGAAAA TAGTCAAATA TGGGATGGGA GGACTGATCA TTTTCTTCTT GATCTGCATC 
ATTTGGTTTC CTCTTCTTTT CATTTCGCTG GTCAGATCGG TGGTGGGCGT GGTCAACCAC 
CCCATCGACG TGACCGTCAC CATCAAGCTG GGAGGGTACG AGCCCCTCTT CACCATGAGT 
GTGCAGCAAC AGTCCATCCA GCCCTTTACA ATGGTTGAAT ATGAACAACT CACAAAGAAG 
TTTGGGAACA ATGCGGCGGC CATGCAGTTT ATAACTCTGT ATAATTATGA GGACATTGTG 
ACGGCCATGA TTGAAGGTAG TTCTGGATCA GTGTGGAGGA TCAGCCCCCC GAGCAGACAG 
GAAGTCATAA AGGAACTGCT GAGTAGCCGT GCTGACCTCA CGCTGCGCCT GGCCTGGAAT 
TTCCAAAGGG ACCTGGGCAA GGGAGGGACG GTTGAACACT CCTTTGACAA GCACTCAGTC 
CCTCTGGAGC CAGGGAACCC CGTCAGGGCT GATATGGCCT CGTTGCTGCT CGGCAATCGT 
ACCGAGCCGG TCCACGTCCC TAATTTGTTC CCGAACTATC TACGTGCCCC CAATGGAGCT 
GAAGCCAAAC CTGTCAGTCA GCTATTTAAA GGTGGTGAAG AAGATTTTCT GAATATAACC 
CTGTCCCTGA AGAGTGACAG GTCCCTGAAC GGGGGGGTGA ACCAGGAGTG GTGGGATATT 
GCCATTGAAG GTTGTGCCCC CTCCTCCTGT GGGGTTCTGC CCATGGTCAT ATTTAATGAC 
AAAGTCAGTC CACCAAGTCT GGGCTTCCTT GCTGGATATG GGATCATGGG TTTGTATGTT 
TCCGTGGTCT TAGTCATCGG GAAATTTGTG CGTGGCTTCT TCAGTGAAAT CTCACACTCC 
ATTATGTTTG AGGAGTTGCC CTGTGTGGAC CGAATCCTGA AGCTGTGCAC CGATATCTTC 
CTGGTTCGTG AGACAGGAGA GCTGGAACTA GAAGAGGAAC TTTACTCTAA GCTGATCTTC 
CTCTATCGCT CTCCAGAGAC GATGATCAAA TGGACCAGGG ACATTAATGC CCAAGACCAG 
AATCCAGGAA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PHONE

1-877-436-7274

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LOC101071632 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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