afap1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	afap1
Entrez Gene ID	101062316
Full Name	actin filament associated protein 1
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011606464.1	XP_011604766.1	actin filament-associated protein 1
XM_011606464.2	XP_011604766.2	actin filament-associated protein 1 isoform X3
XM_029840313.1	XP_029696173.1	actin filament-associated protein 1 isoform X1
XM_029840315.1	XP_029696175.1	actin filament-associated protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following afap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the afap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo19500	XM_011606464.1 Latest version!	Takifugu rubripes actin filament associated protein 1 (afap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo71201	XM_029840313.1 Latest version!	Takifugu rubripes actin filament associated protein 1 (afap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo71202	XM_029840315.1 Latest version!	Takifugu rubripes actin filament associated protein 1 (afap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo71203	XM_011606464.2 Latest version!	Takifugu rubripes actin filament associated protein 1 (afap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

afap1 ( XM_011606464.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604766.1 Takifugu rubripes actin filament associated protein 1 (afap1), mRNA.

Price & Availability↑

CloneID	OTo19500
Clone ID Related Accession (Same CDS sequence)	XM_011606464.1
Accession Version	XM_011606464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	actin filament-associated protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071000.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG AGCCCGTTCT CTTCACTTCC AACAGGGCGA TGGAGGAGCT GCTGTGCGAG 
CTTCGTCTCT TCCTGGACCT GTTGGACAGG GAGTACCTGA GTGCCGGGGT CCGAGAGAGG 
AAGCTGCACC TCTCCAACAT CCTCCACAGA GTCCTGTCGG AGAGAGAGCC CTCATTCAAG 
CCGGAGACAC CGAGTGGTCT GCCGGCCCCT CCCCAGATGC CCCTTCCTGA GATTCCTCAG 
CCCTGGCTGC CTCCGAACAG CGGCCCTCCG CCTCTGCCGA GCTCCTCCCT ACCTGAAGGT 
TACTACGAAG AGGCGGTTCC TTTAGGTCCT GGAAAAGCTC CAGAATACAT CACCTCCCAC 
TACGACTCGG ACGCCATGAG CAGCTCCTAC GAGTCCTATG ATGAAGAAGA GGAGGACGGC 
AAAGGTCAGA AGATGCGTCA CCAGTGGCCG TCAGAGGAGG CGTCCATGGA CTTGGTGAAG 
GACGCTCGGA TCTGCGCCTT CCTGCTGCGT AAGAAGCGCT TCGGCCAGTG GACCAAACTG 
CTGTGTGTCA TCAAAGACAA CAAGCTGCTG TGTTACAAGT CCTCCAAAGA CCACACCCCC 
CAGATGGAGC TGATCTTGTC TGGGTGCAGC GTCACGCACG TCCCCAAAGA CGGCAAGAAG 
AAGAAGCACG AGCTGAAGAT CGTCCACCAG GGGGCAGACC CTCTGGTGCT AGCCGTGCAG 
AGCAAAGAGC AGGCCGAGGA GTGGCTCAAG GTGATGAAAG AAGTTTGCGT TAATGGGAAC 
GTGGATCTGG ACCGAGTTGG ATCTGGGTCC CCGGTTCACA AACCTGAGCT GGAAAAGAAG 
GCTTCATGTG ACAGGCCCAG CTCTGACGGC GAGCTGACTC ATGAGAACGG ACACAGCGAC 
TGTAAGGACC AAGGAAAAGG AAAGAAGAGC TCCAAGTCGG AGCAGAAGAG CGGGACAGTG 
GGGAAGGGGG CAGGCAAGAC CATCACGAAG ATCATCGGCC TGGGGAAGAA GAAGCCGTCG 
GTGGACGAGC AGACGTCGTC GGCGGAGGAG GACGTGCCCA CGTGTGGTTA CCTTAACGTC 
CTGTCCAACA ACCGCTGGCG GGAGCGCTGG TGCCGCCTGA AGGACAACCA GCTGCTGCTC 
CACAAAGACC GAGACGACCT GAAGAGCCAC ATCGCCTCGC TGCCCCTGCG CGGCTGCGAG 
GTCAGCCCCG GCCTCGACCA CAAGCACCCG TTTGCCTTCC GCCTGCTGCG TAACGGCCAG 
GAGGTGGCGG TGCTGGAGGC GTCCTCCTCA GAGGCGATGG GGCGGTGGTT GGGGGTCTTA 
CTGGCTGAGA CCGGCTCCAC CACTGATCCA GCCACGCTGC ACTACGACTA CATCGACGTG 
GAGACAACAG CCAACATCAT CCAGCTGGCC AAGCAGTCCT TCTGCTTCAC CAGCAAGCGC 
GCTGTGTCTC CAAACCCTTA CCTGGACAAC CCGGTCAACG GCTACGCCTG TCCCACTGGG 
ATGGCCCTGC ACTATGATGA TGTGCCCATT AATGGGACGG TGAAGGGATC AAAGGGATTG 
ACCAACGGGT TGTCGTGGAA ACAGAAGGTG GATCCAAAGA AGCAGGTGAA AAAGAGCGCG 
GTGAACGTGA TGAACGGTAC GGGCTCGCTC AAACGCAACG GCTCCGACGC AGACCAGTGC 
AAGTACGGGA AGAACCGCGT GGAGGCGGAC GCCAGGCGGC TGCAGTGCAA AGAGGAAGAG 
CTGATGAAGA GGAAGCAGGA GATCCGGAGC CACCTGAGCC AGCTGAAGAA GGACAGGAGG 
GACATGAAAG CAGCTCTGGA AACCTCCACA GGAAAACGTT CCCACGCTTT GCTGGCGGAG 
CGGCTGAAGA AGACGGAGGA GGAGTGCAAA CAGAAGGAGG AGGAGTGCGT GAACCTGGAG 
CTGGAGCTGA CCGAGGTGAA GGAGAACCTG AAGAAAGCTC TGAGTGGAGG CGTCACCCTG 
GGCCTGACCA TCCAGCCCAA ATCCGGATCC TCGGGCCTCC AGTCGCCCGC CGTGTCGCGG 
CGGACTCTCG AGTCCTCGCC GTTTTCCAGC TGCGACACCA GCGACACGGA GTCGTGCTCC 
CTGCCCGTCA ACAGCGCCTC GCTGCTCCGC CGGCAGCCGC CCGGCCAGAA GCCCTCGGCG 
GTGCGGGGAC ACGTCCTGAG GAAGGCCAAG GAATGGGAGC AGAGTACGGC ACATAAACTC 
CTCCGTCTCC ATGTTTTCAC TCTTTTACGT CAAACAAATT GTACATATTT ATTTAAACGT 
CTCCATCCAG CCTTTGAGGA GGACGCGGGT CACGTGACCA GATCCCGCCG GCGCCGCGCC 
AGCAGACGTT TGTCTTTTAG TTCCCACCTG CAGGATTTGA GACGGTGGAA GCGCCGCTAT 
TTATTTGTAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604766.1
CDS	198..2615
Translation	MRSEPVLFTSNRAMEELLCELRLFLDLLDREYLSAGVRERKLHLSNILHRVLSEREPSFKPETPSGLPAPPQMPLPEIPQPWLPPNSGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSHYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDHTPQMELILSGCSVTHVPKDGKKKKHELKIVHQGADPLVLAVQSKEQAEEWLKVMKEVCVNGNVDLDRVGSGSPVHKPELEKKASCDRPSSDGELTHENGHSDCKDQGKGKKSSKSEQKSGTVGKGAGKTITKIIGLGKKKPSVDEQTSSAEEDVPTCGYLNVLSNNRWRERWCRLKDNQLLLHKDRDDLKSHIASLPLRGCEVSPGLDHKHPFAFRLLRNGQEVAVLEASSSEAMGRWLGVLLAETGSTTDPATLHYDYIDVETTANIIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGMALHYDDVPINGTVKGSKGLTNGLSWKQKVDPKKQVKKSAVNVMNGTGSLKRNGSDADQCKYGKNRVEADARRLQCKEEELMKRKQEIRSHLSQLKKDRRDMKAALETSTGKRSHALLAERLKKTEEECKQKEEECVNLELELTEVKENLKKALSGGVTLGLTIQPKSGSSGLQSPAVSRRTLESSPFSSCDTSDTESCSLPVNSASLLRRQPPGQKPSAVRGHVLRKAKEWEQSTAHKLLRLHVFTLLRQTNCTYLFKRLHPAFEEDAGHVTRSRRRRASRRLSFSSHLQDLRRWKRRYLFVNV

Target ORF information:

RefSeq Version	XM_011606464.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes actin filament associated protein 1 (afap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606464.1

ORF Insert Sequence:

ATGCGGAGCG AGCCCGTTCT CTTCACTTCC AACAGGGCGA TGGAGGAGCT GCTGTGCGAG 
CTTCGTCTCT TCCTGGACCT GTTGGACAGG GAGTACCTGA GTGCCGGGGT CCGAGAGAGG 
AAGCTGCACC TCTCCAACAT CCTCCACAGA GTCCTGTCGG AGAGAGAGCC CTCATTCAAG 
CCGGAGACAC CGAGTGGTCT GCCGGCCCCT CCCCAGATGC CCCTTCCTGA GATTCCTCAG 
CCCTGGCTGC CTCCGAACAG CGGCCCTCCG CCTCTGCCGA GCTCCTCCCT ACCTGAAGGT 
TACTACGAAG AGGCGGTTCC TTTAGGTCCT GGAAAAGCTC CAGAATACAT CACCTCCCAC 
TACGACTCGG ACGCCATGAG CAGCTCCTAC GAGTCCTATG ATGAAGAAGA GGAGGACGGC 
AAAGGTCAGA AGATGCGTCA CCAGTGGCCG TCAGAGGAGG CGTCCATGGA CTTGGTGAAG 
GACGCTCGGA TCTGCGCCTT CCTGCTGCGT AAGAAGCGCT TCGGCCAGTG GACCAAACTG 
CTGTGTGTCA TCAAAGACAA CAAGCTGCTG TGTTACAAGT CCTCCAAAGA CCACACCCCC 
CAGATGGAGC TGATCTTGTC TGGGTGCAGC GTCACGCACG TCCCCAAAGA CGGCAAGAAG 
AAGAAGCACG AGCTGAAGAT CGTCCACCAG GGGGCAGACC CTCTGGTGCT AGCCGTGCAG 
AGCAAAGAGC AGGCCGAGGA GTGGCTCAAG GTGATGAAAG AAGTTTGCGT TAATGGGAAC 
GTGGATCTGG ACCGAGTTGG ATCTGGGTCC CCGGTTCACA AACCTGAGCT GGAAAAGAAG 
GCTTCATGTG ACAGGCCCAG CTCTGACGGC GAGCTGACTC ATGAGAACGG ACACAGCGAC 
TGTAAGGACC AAGGAAAAGG AAAGAAGAGC TCCAAGTCGG AGCAGAAGAG CGGGACAGTG 
GGGAAGGGGG CAGGCAAGAC CATCACGAAG ATCATCGGCC TGGGGAAGAA GAAGCCGTCG 
GTGGACGAGC AGACGTCGTC GGCGGAGGAG GACGTGCCCA CGTGTGGTTA CCTTAACGTC 
CTGTCCAACA ACCGCTGGCG GGAGCGCTGG TGCCGCCTGA AGGACAACCA GCTGCTGCTC 
CACAAAGACC GAGACGACCT GAAGAGCCAC ATCGCCTCGC TGCCCCTGCG CGGCTGCGAG 
GTCAGCCCCG GCCTCGACCA CAAGCACCCG TTTGCCTTCC GCCTGCTGCG TAACGGCCAG 
GAGGTGGCGG TGCTGGAGGC GTCCTCCTCA GAGGCGATGG GGCGGTGGTT GGGGGTCTTA 
CTGGCTGAGA CCGGCTCCAC CACTGATCCA GCCACGCTGC ACTACGACTA CATCGACGTG 
GAGACAACAG CCAACATCAT CCAGCTGGCC AAGCAGTCCT TCTGCTTCAC CAGCAAGCGC 
GCTGTGTCTC CAAACCCTTA CCTGGACAAC CCGGTCAACG GCTACGCCTG TCCCACTGGG 
ATGGCCCTGC ACTATGATGA TGTGCCCATT AATGGGACGG TGAAGGGATC AAAGGGATTG 
ACCAACGGGT TGTCGTGGAA ACAGAAGGTG GATCCAAAGA AGCAGGTGAA AAAGAGCGCG 
GTGAACGTGA TGAACGGTAC GGGCTCGCTC AAACGCAACG GCTCCGACGC AGACCAGTGC 
AAGTACGGGA AGAACCGCGT GGAGGCGGAC GCCAGGCGGC TGCAGTGCAA AGAGGAAGAG 
CTGATGAAGA GGAAGCAGGA GATCCGGAGC CACCTGAGCC AGCTGAAGAA GGACAGGAGG 
GACATGAAAG CAGCTCTGGA AACCTCCACA GGAAAACGTT CCCACGCTTT GCTGGCGGAG 
CGGCTGAAGA AGACGGAGGA GGAGTGCAAA CAGAAGGAGG AGGAGTGCGT GAACCTGGAG 
CTGGAGCTGA CCGAGGTGAA GGAGAACCTG AAGAAAGCTC TGAGTGGAGG CGTCACCCTG 
GGCCTGACCA TCCAGCCCAA ATCCGGATCC TCGGGCCTCC AGTCGCCCGC CGTGTCGCGG 
CGGACTCTCG AGTCCTCGCC GTTTTCCAGC TGCGACACCA GCGACACGGA GTCGTGCTCC 
CTGCCCGTCA ACAGCGCCTC GCTGCTCCGC CGGCAGCCGC CCGGCCAGAA GCCCTCGGCG 
GTGCGGGGAC ACGTCCTGAG GAAGGCCAAG GAATGGGAGC AGAGTACGGC ACATAAACTC 
CTCCGTCTCC ATGTTTTCAC TCTTTTACGT CAAACAAATT GTACATATTT ATTTAAACGT 
CTCCATCCAG CCTTTGAGGA GGACGCGGGT CACGTGACCA GATCCCGCCG GCGCCGCGCC 
AGCAGACGTT TGTCTTTTAG TTCCCACCTG CAGGATTTGA GACGGTGGAA GCGCCGCTAT 
TTATTTGTAA ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_029840313.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696173.1 Takifugu rubripes actin filament associated protein 1 (afap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo71201
Clone ID Related Accession (Same CDS sequence)	XM_029840313.1
Accession Version	XM_029840313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	actin filament-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG AGCCCGTTCT CTTCACTTCC AACAGGGCGA TGGAGGAGCT GCTGTGCGAG 
CTTCGTCTCT TCCTGGACCT GTTGGACAGG GAGTACCTGA GTGCCGGGGT CCGAGAGAGG 
AAGCTGCACC TCTCCAACAT CCTCCACAGA GTCCTGTCGG AGAGAGAGCC CTCATTCAAG 
CCGGAGACAC CGAGTGGTCT GCCGGCCCCT CCCCAGATGC CCCTTCCTGA GATTCCTCAG 
CCCTGGCTGC CTCCGAACAG CGGCCCTCCG CCTCTGCCCA GCTCCTCCCT ACCTGAAGGT 
TACTACGAAG AGGCGGTTCC TTTAGGTCCT GGAAAAGCTC CAGAATACAT CACCTCCCAC 
TACGACTCGG ACGCCATGAG CAGCTCCTAC GAGTCCTACG ATGAAGAAGA GGAGGACGGC 
AAAGGTCAGA AGATGCGTCA CCAGTGGCCG TCAGAGGAGG CGTCCATGGA CTTGGTGAAG 
GACGCTCGGA TCTGCGCCTT CCTGCTGCGT AAGAAGCGCT TCGGCCAGTG GACCAAACTG 
CTGTGTGTCA TCAAAGACAA CAAGCTGCTG TGTTACAAGT CCTCCAAAGA CCACACCCCC 
CAGATGGAGC TGATCTTGTC TGGGTGCAGC GTCACGCACG TCCCCAAAGA CGGCAAGAAG 
AAGAAGCACG AGCTGAAGAT CGTCCACCAG GGGGCAGACC CTCTGGTGCT AGCCGTGCAG 
AGCAAAGAGC AGGCCGAGGA GTGGCTCAAG GTGATGAAAG AAGTTTGCGT TAATGGGAAC 
GTGGATCTGG ACCGAGTTGG ATCTGGATCC CCGGTTCACA AACCTGAGCT GGAAAAGAAG 
GCTTCATGTG ACAGGCCCAG CTCTGACGGC GAGCTGACTC ATGAGAACGG ACACAGCGAC 
TGTAAGGACC AAGGAAAAGG AAAGAAGAGC TCCAAGTCGG AGCAGAAGAG CGGGACAGTG 
GGGAAGGGGG CAGGCAAGAC CATCACGAAG ATCATCGGCC TGGGGAAGAA GAAGCCGTCG 
GTGGACGAGC AGACGTCGTC GGCGGAGGAG GACGTGCCCA CGTGTGGTTA CCTTAACGTC 
CTGTCCAACA ACCGCTGGCG GGAGCGCTGG TGCCGCCTGA AGGACAACCA GCTGCTGCTC 
CACAAAGACC GAGACGACCT GAAGAGCCAC ATCGCCTCGC TGCCCCTGCG CGGCTGCGAG 
GTCAGCCCCG GCCTCGACCA CAAGCACCCG TTTGCCTTCC GCCTGCTGCG TAACGGCCAG 
GAGGTGGCGG TGCTGGAGGC GTCCTCCTCA GAGGCGATGG GGCGGTGGTT GGGGGTCTTA 
CTGGCTGAGA CCGGCTCCAC CACTGATCCA GCCACGCTGC ACTACGACTA CATCGACGTG 
GAGACAACAG CCAACATCAT CCAGCTGGCC AAGCAGTCCT TCTGCTTCAC CAGCAAGCGC 
GCTGTGTCTC CAAACCCTTA CCTGGACAAC CCGGTCAACG GCTACGCCTG TCCCACTGGG 
ATGGCCCTGC ACTATGATGA TGTGCCCATT AATGGGACGA ACCTGGAGGC GCTAAACTCC 
AGCTCCAACA CTGGGGGGCG CCAGCTGAAA GAGCCGTTAC ACTATGAGAA CACTGACGTG 
TATGAGAACG TGCCTTCACC TGAGCTGGCT GCTCGCTGTC CTGCAAAACC ACCTCCTTCT 
TCTTCTTCTT CTTCTGTTTG CTCTAACAGT CACTACAAAA CACCTCAAGG CTCTAAAGTC 
TCCTCTAAGT TTCTGTCTGA GGACACCAAA GTGAAGAACG CAGTGAAGGT GAAGGGATCA 
AAGGGATTGA CCAACGGGTT GTCGTGGAAA CAGAAGGTGG ATCCAAAGAA GCAGGTGAAA 
AAGAGCGCGG TGAACGTGAT GAACGGTACG GGCTCGCTCA AACGCAACGG CTCCGACGCA 
GACCAGTGCA AGTACGGGAA GAACCGCGTG GAGGCGGACG CCAGGCGGCT GCAGTGCAAA 
GAGGAAGAGC TGATGAAGAG GAAGCAGGAG ATCCGGAGCC ACCTGAGCCA GCTGAAGAAG 
GACAGGAGGG ACATGAAAGC AGCTCTGGAA ACCTCCACAG GAAAACGTTC CCACGCTTTG 
CTGGCGGAGC GGCTGAAGAA GACGGAGGAG GAGTGCAAAC AGAAGGAGGA GGAGTGCGTG 
AACCTGGAGC TGGAGCTGAC CGAGGTGAAG GAGAACCTGA AGAAAGCTCT GAGTGGAGGC 
GTCACCCTGG GCCTGACCAT CCAGCCCAAA TCCGGATCCT CGGGCCTCCA GTCGCCCGCC 
GTGTCGCGGC GGACTCTCGA GTCCTCGCCG TTTTCCAGCT GCGACACCAG CGACACGGAG 
TCGTGCTCCC TGCCCGTCAA CAGCGCCTCG CTGCTCCGCC GGCAGCCGCC CGGCCAGAAG 
CCCTCGGCGG TGCGGGGACA CGTCCTGAGG AAGGCCAAGG AATGGGAGCA GAAGAACGGC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696173.1
CDS	208..2673
Translation	MRSEPVLFTSNRAMEELLCELRLFLDLLDREYLSAGVRERKLHLSNILHRVLSEREPSFKPETPSGLPAPPQMPLPEIPQPWLPPNSGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSHYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDHTPQMELILSGCSVTHVPKDGKKKKHELKIVHQGADPLVLAVQSKEQAEEWLKVMKEVCVNGNVDLDRVGSGSPVHKPELEKKASCDRPSSDGELTHENGHSDCKDQGKGKKSSKSEQKSGTVGKGAGKTITKIIGLGKKKPSVDEQTSSAEEDVPTCGYLNVLSNNRWRERWCRLKDNQLLLHKDRDDLKSHIASLPLRGCEVSPGLDHKHPFAFRLLRNGQEVAVLEASSSEAMGRWLGVLLAETGSTTDPATLHYDYIDVETTANIIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGMALHYDDVPINGTNLEALNSSSNTGGRQLKEPLHYENTDVYENVPSPELAARCPAKPPPSSSSSSVCSNSHYKTPQGSKVSSKFLSEDTKVKNAVKVKGSKGLTNGLSWKQKVDPKKQVKKSAVNVMNGTGSLKRNGSDADQCKYGKNRVEADARRLQCKEEELMKRKQEIRSHLSQLKKDRRDMKAALETSTGKRSHALLAERLKKTEEECKQKEEECVNLELELTEVKENLKKALSGGVTLGLTIQPKSGSSGLQSPAVSRRTLESSPFSSCDTSDTESCSLPVNSASLLRRQPPGQKPSAVRGHVLRKAKEWEQKNGT

Target ORF information:

RefSeq Version	XM_029840313.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes actin filament associated protein 1 (afap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840313.1

ORF Insert Sequence:

ATGCGGAGCG AGCCCGTTCT CTTCACTTCC AACAGGGCGA TGGAGGAGCT GCTGTGCGAG 
CTTCGTCTCT TCCTGGACCT GTTGGACAGG GAGTACCTGA GTGCCGGGGT CCGAGAGAGG 
AAGCTGCACC TCTCCAACAT CCTCCACAGA GTCCTGTCGG AGAGAGAGCC CTCATTCAAG 
CCGGAGACAC CGAGTGGTCT GCCGGCCCCT CCCCAGATGC CCCTTCCTGA GATTCCTCAG 
CCCTGGCTGC CTCCGAACAG CGGCCCTCCG CCTCTGCCCA GCTCCTCCCT ACCTGAAGGT 
TACTACGAAG AGGCGGTTCC TTTAGGTCCT GGAAAAGCTC CAGAATACAT CACCTCCCAC 
TACGACTCGG ACGCCATGAG CAGCTCCTAC GAGTCCTACG ATGAAGAAGA GGAGGACGGC 
AAAGGTCAGA AGATGCGTCA CCAGTGGCCG TCAGAGGAGG CGTCCATGGA CTTGGTGAAG 
GACGCTCGGA TCTGCGCCTT CCTGCTGCGT AAGAAGCGCT TCGGCCAGTG GACCAAACTG 
CTGTGTGTCA TCAAAGACAA CAAGCTGCTG TGTTACAAGT CCTCCAAAGA CCACACCCCC 
CAGATGGAGC TGATCTTGTC TGGGTGCAGC GTCACGCACG TCCCCAAAGA CGGCAAGAAG 
AAGAAGCACG AGCTGAAGAT CGTCCACCAG GGGGCAGACC CTCTGGTGCT AGCCGTGCAG 
AGCAAAGAGC AGGCCGAGGA GTGGCTCAAG GTGATGAAAG AAGTTTGCGT TAATGGGAAC 
GTGGATCTGG ACCGAGTTGG ATCTGGATCC CCGGTTCACA AACCTGAGCT GGAAAAGAAG 
GCTTCATGTG ACAGGCCCAG CTCTGACGGC GAGCTGACTC ATGAGAACGG ACACAGCGAC 
TGTAAGGACC AAGGAAAAGG AAAGAAGAGC TCCAAGTCGG AGCAGAAGAG CGGGACAGTG 
GGGAAGGGGG CAGGCAAGAC CATCACGAAG ATCATCGGCC TGGGGAAGAA GAAGCCGTCG 
GTGGACGAGC AGACGTCGTC GGCGGAGGAG GACGTGCCCA CGTGTGGTTA CCTTAACGTC 
CTGTCCAACA ACCGCTGGCG GGAGCGCTGG TGCCGCCTGA AGGACAACCA GCTGCTGCTC 
CACAAAGACC GAGACGACCT GAAGAGCCAC ATCGCCTCGC TGCCCCTGCG CGGCTGCGAG 
GTCAGCCCCG GCCTCGACCA CAAGCACCCG TTTGCCTTCC GCCTGCTGCG TAACGGCCAG 
GAGGTGGCGG TGCTGGAGGC GTCCTCCTCA GAGGCGATGG GGCGGTGGTT GGGGGTCTTA 
CTGGCTGAGA CCGGCTCCAC CACTGATCCA GCCACGCTGC ACTACGACTA CATCGACGTG 
GAGACAACAG CCAACATCAT CCAGCTGGCC AAGCAGTCCT TCTGCTTCAC CAGCAAGCGC 
GCTGTGTCTC CAAACCCTTA CCTGGACAAC CCGGTCAACG GCTACGCCTG TCCCACTGGG 
ATGGCCCTGC ACTATGATGA TGTGCCCATT AATGGGACGA ACCTGGAGGC GCTAAACTCC 
AGCTCCAACA CTGGGGGGCG CCAGCTGAAA GAGCCGTTAC ACTATGAGAA CACTGACGTG 
TATGAGAACG TGCCTTCACC TGAGCTGGCT GCTCGCTGTC CTGCAAAACC ACCTCCTTCT 
TCTTCTTCTT CTTCTGTTTG CTCTAACAGT CACTACAAAA CACCTCAAGG CTCTAAAGTC 
TCCTCTAAGT TTCTGTCTGA GGACACCAAA GTGAAGAACG CAGTGAAGGT GAAGGGATCA 
AAGGGATTGA CCAACGGGTT GTCGTGGAAA CAGAAGGTGG ATCCAAAGAA GCAGGTGAAA 
AAGAGCGCGG TGAACGTGAT GAACGGTACG GGCTCGCTCA AACGCAACGG CTCCGACGCA 
GACCAGTGCA AGTACGGGAA GAACCGCGTG GAGGCGGACG CCAGGCGGCT GCAGTGCAAA 
GAGGAAGAGC TGATGAAGAG GAAGCAGGAG ATCCGGAGCC ACCTGAGCCA GCTGAAGAAG 
GACAGGAGGG ACATGAAAGC AGCTCTGGAA ACCTCCACAG GAAAACGTTC CCACGCTTTG 
CTGGCGGAGC GGCTGAAGAA GACGGAGGAG GAGTGCAAAC AGAAGGAGGA GGAGTGCGTG 
AACCTGGAGC TGGAGCTGAC CGAGGTGAAG GAGAACCTGA AGAAAGCTCT GAGTGGAGGC 
GTCACCCTGG GCCTGACCAT CCAGCCCAAA TCCGGATCCT CGGGCCTCCA GTCGCCCGCC 
GTGTCGCGGC GGACTCTCGA GTCCTCGCCG TTTTCCAGCT GCGACACCAG CGACACGGAG 
TCGTGCTCCC TGCCCGTCAA CAGCGCCTCG CTGCTCCGCC GGCAGCCGCC CGGCCAGAAG 
CCCTCGGCGG TGCGGGGACA CGTCCTGAGG AAGGCCAAGG AATGGGAGCA GAAGAACGGC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_029840315.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696175.1 Takifugu rubripes actin filament associated protein 1 (afap1), transcript variant X2, mRNA.

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CloneID	OTo71202
Clone ID Related Accession (Same CDS sequence)	XM_029840315.1
Accession Version	XM_029840315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	actin filament-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAA AAGAGGAAAA CGGCAAAGCG ATGGAGGAGC TGCTGTGCGA GCTTCGTCTC 
TTCCTGGACC TGTTGGACAG GGAGTACCTG AGTGCCGGGG TCCGAGAGAG GAAGCTGCAC 
CTCTCCAACA TCCTCCACAG AGTCCTGTCG GAGAGAGAGC CCTCATTCAA GCCGGAGACA 
CCGAGTGGTC TGCCGGCCCC TCCCCAGATG CCCCTTCCTG AGATTCCTCA GCCCTGGCTG 
CCTCCGAACA GCGGCCCTCC GCCTCTGCCC AGCTCCTCCC TACCTGAAGG TTACTACGAA 
GAGGCGGTTC CTTTAGGTCC TGGAAAAGCT CCAGAATACA TCACCTCCCA CTACGACTCG 
GACGCCATGA GCAGCTCCTA CGAGTCCTAC GATGAAGAAG AGGAGGACGG CAAAGGTCAG 
AAGATGCGTC ACCAGTGGCC GTCAGAGGAG GCGTCCATGG ACTTGGTGAA GGACGCTCGG 
ATCTGCGCCT TCCTGCTGCG TAAGAAGCGC TTCGGCCAGT GGACCAAACT GCTGTGTGTC 
ATCAAAGACA ACAAGCTGCT GTGTTACAAG TCCTCCAAAG ACCACACCCC CCAGATGGAG 
CTGATCTTGT CTGGGTGCAG CGTCACGCAC GTCCCCAAAG ACGGCAAGAA GAAGAAGCAC 
GAGCTGAAGA TCGTCCACCA GGGGGCAGAC CCTCTGGTGC TAGCCGTGCA GAGCAAAGAG 
CAGGCCGAGG AGTGGCTCAA GGTGATGAAA GAAGTTTGCG TTAATGGGAA CGTGGATCTG 
GACCGAGTTG GATCTGGATC CCCGGTTCAC AAACCTGAGC TGGAAAAGAA GGCTTCATGT 
GACAGGCCCA GCTCTGACGG CGAGCTGACT CATGAGAACG GACACAGCGA CTGTAAGGAC 
CAAGGAAAAG GAAAGAAGAG CTCCAAGTCG GAGCAGAAGA GCGGGACAGT GGGGAAGGGG 
GCAGGCAAGA CCATCACGAA GATCATCGGC CTGGGGAAGA AGAAGCCGTC GGTGGACGAG 
CAGACGTCGT CGGCGGAGGA GGACGTGCCC ACGTGTGGTT ACCTTAACGT CCTGTCCAAC 
AACCGCTGGC GGGAGCGCTG GTGCCGCCTG AAGGACAACC AGCTGCTGCT CCACAAAGAC 
CGAGACGACC TGAAGAGCCA CATCGCCTCG CTGCCCCTGC GCGGCTGCGA GGTCAGCCCC 
GGCCTCGACC ACAAGCACCC GTTTGCCTTC CGCCTGCTGC GTAACGGCCA GGAGGTGGCG 
GTGCTGGAGG CGTCCTCCTC AGAGGCGATG GGGCGGTGGT TGGGGGTCTT ACTGGCTGAG 
ACCGGCTCCA CCACTGATCC AGCCACGCTG CACTACGACT ACATCGACGT GGAGACAACA 
GCCAACATCA TCCAGCTGGC CAAGCAGTCC TTCTGCTTCA CCAGCAAGCG CGCTGTGTCT 
CCAAACCCTT ACCTGGACAA CCCGGTCAAC GGCTACGCCT GTCCCACTGG GATGGCCCTG 
CACTATGATG ATGTGCCCAT TAATGGGACG AACCTGGAGG CGCTAAACTC CAGCTCCAAC 
ACTGGGGGGC GCCAGCTGAA AGAGCCGTTA CACTATGAGA ACACTGACGT GTATGAGAAC 
GTGCCTTCAC CTGAGCTGGC TGCTCGCTGT CCTGCAAAAC CACCTCCTTC TTCTTCTTCT 
TCTTCTGTTT GCTCTAACAG TCACTACAAA ACACCTCAAG GCTCTAAAGT CTCCTCTAAG 
TTTCTGTCTG AGGACACCAA AGTGAAGAAC GCAGTGAAGG TGAAGGGATC AAAGGGATTG 
ACCAACGGGT TGTCGTGGAA ACAGAAGGTG GATCCAAAGA AGCAGGTGAA AAAGAGCGCG 
GTGAACGTGA TGAACGGTAC GGGCTCGCTC AAACGCAACG GCTCCGACGC AGACCAGTGC 
AAGTACGGGA AGAACCGCGT GGAGGCGGAC GCCAGGCGGC TGCAGTGCAA AGAGGAAGAG 
CTGATGAAGA GGAAGCAGGA GATCCGGAGC CACCTGAGCC AGCTGAAGAA GGACAGGAGG 
GACATGAAAG CAGCTCTGGA AACCTCCACA GGAAAACGTT CCCACGCTTT GCTGGCGGAG 
CGGCTGAAGA AGACGGAGGA GGAGTGCAAA CAGAAGGAGG AGGAGTGCGT GAACCTGGAG 
CTGGAGCTGA CCGAGGTGAA GGAGAACCTG AAGAAAGCTC TGAGTGGAGG CGTCACCCTG 
GGCCTGACCA TCCAGCCCAA ATCCGGATCC TCGGGCCTCC AGTCGCCCGC CGTGTCGCGG 
CGGACTCTCG AGTCCTCGCC GTTTTCCAGC TGCGACACCA GCGACACGGA GTCGTGCTCC 
CTGCCCGTCA ACAGCGCCTC GCTGCTCCGC CGGCAGCCGC CCGGCCAGAA GCCCTCGGCG 
GTGCGGGGAC ACGTCCTGAG GAAGGCCAAG GAATGGGAGC AGAAGAACGG CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696175.1
CDS	112..2568
Translation	MMEKEENGKAMEELLCELRLFLDLLDREYLSAGVRERKLHLSNILHRVLSEREPSFKPETPSGLPAPPQMPLPEIPQPWLPPNSGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSHYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDHTPQMELILSGCSVTHVPKDGKKKKHELKIVHQGADPLVLAVQSKEQAEEWLKVMKEVCVNGNVDLDRVGSGSPVHKPELEKKASCDRPSSDGELTHENGHSDCKDQGKGKKSSKSEQKSGTVGKGAGKTITKIIGLGKKKPSVDEQTSSAEEDVPTCGYLNVLSNNRWRERWCRLKDNQLLLHKDRDDLKSHIASLPLRGCEVSPGLDHKHPFAFRLLRNGQEVAVLEASSSEAMGRWLGVLLAETGSTTDPATLHYDYIDVETTANIIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGMALHYDDVPINGTNLEALNSSSNTGGRQLKEPLHYENTDVYENVPSPELAARCPAKPPPSSSSSSVCSNSHYKTPQGSKVSSKFLSEDTKVKNAVKVKGSKGLTNGLSWKQKVDPKKQVKKSAVNVMNGTGSLKRNGSDADQCKYGKNRVEADARRLQCKEEELMKRKQEIRSHLSQLKKDRRDMKAALETSTGKRSHALLAERLKKTEEECKQKEEECVNLELELTEVKENLKKALSGGVTLGLTIQPKSGSSGLQSPAVSRRTLESSPFSSCDTSDTESCSLPVNSASLLRRQPPGQKPSAVRGHVLRKAKEWEQKNGT

Target ORF information:

RefSeq Version	XM_029840315.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes actin filament associated protein 1 (afap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840315.1

ORF Insert Sequence:

ATGATGGAAA AAGAGGAAAA CGGCAAAGCG ATGGAGGAGC TGCTGTGCGA GCTTCGTCTC 
TTCCTGGACC TGTTGGACAG GGAGTACCTG AGTGCCGGGG TCCGAGAGAG GAAGCTGCAC 
CTCTCCAACA TCCTCCACAG AGTCCTGTCG GAGAGAGAGC CCTCATTCAA GCCGGAGACA 
CCGAGTGGTC TGCCGGCCCC TCCCCAGATG CCCCTTCCTG AGATTCCTCA GCCCTGGCTG 
CCTCCGAACA GCGGCCCTCC GCCTCTGCCC AGCTCCTCCC TACCTGAAGG TTACTACGAA 
GAGGCGGTTC CTTTAGGTCC TGGAAAAGCT CCAGAATACA TCACCTCCCA CTACGACTCG 
GACGCCATGA GCAGCTCCTA CGAGTCCTAC GATGAAGAAG AGGAGGACGG CAAAGGTCAG 
AAGATGCGTC ACCAGTGGCC GTCAGAGGAG GCGTCCATGG ACTTGGTGAA GGACGCTCGG 
ATCTGCGCCT TCCTGCTGCG TAAGAAGCGC TTCGGCCAGT GGACCAAACT GCTGTGTGTC 
ATCAAAGACA ACAAGCTGCT GTGTTACAAG TCCTCCAAAG ACCACACCCC CCAGATGGAG 
CTGATCTTGT CTGGGTGCAG CGTCACGCAC GTCCCCAAAG ACGGCAAGAA GAAGAAGCAC 
GAGCTGAAGA TCGTCCACCA GGGGGCAGAC CCTCTGGTGC TAGCCGTGCA GAGCAAAGAG 
CAGGCCGAGG AGTGGCTCAA GGTGATGAAA GAAGTTTGCG TTAATGGGAA CGTGGATCTG 
GACCGAGTTG GATCTGGATC CCCGGTTCAC AAACCTGAGC TGGAAAAGAA GGCTTCATGT 
GACAGGCCCA GCTCTGACGG CGAGCTGACT CATGAGAACG GACACAGCGA CTGTAAGGAC 
CAAGGAAAAG GAAAGAAGAG CTCCAAGTCG GAGCAGAAGA GCGGGACAGT GGGGAAGGGG 
GCAGGCAAGA CCATCACGAA GATCATCGGC CTGGGGAAGA AGAAGCCGTC GGTGGACGAG 
CAGACGTCGT CGGCGGAGGA GGACGTGCCC ACGTGTGGTT ACCTTAACGT CCTGTCCAAC 
AACCGCTGGC GGGAGCGCTG GTGCCGCCTG AAGGACAACC AGCTGCTGCT CCACAAAGAC 
CGAGACGACC TGAAGAGCCA CATCGCCTCG CTGCCCCTGC GCGGCTGCGA GGTCAGCCCC 
GGCCTCGACC ACAAGCACCC GTTTGCCTTC CGCCTGCTGC GTAACGGCCA GGAGGTGGCG 
GTGCTGGAGG CGTCCTCCTC AGAGGCGATG GGGCGGTGGT TGGGGGTCTT ACTGGCTGAG 
ACCGGCTCCA CCACTGATCC AGCCACGCTG CACTACGACT ACATCGACGT GGAGACAACA 
GCCAACATCA TCCAGCTGGC CAAGCAGTCC TTCTGCTTCA CCAGCAAGCG CGCTGTGTCT 
CCAAACCCTT ACCTGGACAA CCCGGTCAAC GGCTACGCCT GTCCCACTGG GATGGCCCTG 
CACTATGATG ATGTGCCCAT TAATGGGACG AACCTGGAGG CGCTAAACTC CAGCTCCAAC 
ACTGGGGGGC GCCAGCTGAA AGAGCCGTTA CACTATGAGA ACACTGACGT GTATGAGAAC 
GTGCCTTCAC CTGAGCTGGC TGCTCGCTGT CCTGCAAAAC CACCTCCTTC TTCTTCTTCT 
TCTTCTGTTT GCTCTAACAG TCACTACAAA ACACCTCAAG GCTCTAAAGT CTCCTCTAAG 
TTTCTGTCTG AGGACACCAA AGTGAAGAAC GCAGTGAAGG TGAAGGGATC AAAGGGATTG 
ACCAACGGGT TGTCGTGGAA ACAGAAGGTG GATCCAAAGA AGCAGGTGAA AAAGAGCGCG 
GTGAACGTGA TGAACGGTAC GGGCTCGCTC AAACGCAACG GCTCCGACGC AGACCAGTGC 
AAGTACGGGA AGAACCGCGT GGAGGCGGAC GCCAGGCGGC TGCAGTGCAA AGAGGAAGAG 
CTGATGAAGA GGAAGCAGGA GATCCGGAGC CACCTGAGCC AGCTGAAGAA GGACAGGAGG 
GACATGAAAG CAGCTCTGGA AACCTCCACA GGAAAACGTT CCCACGCTTT GCTGGCGGAG 
CGGCTGAAGA AGACGGAGGA GGAGTGCAAA CAGAAGGAGG AGGAGTGCGT GAACCTGGAG 
CTGGAGCTGA CCGAGGTGAA GGAGAACCTG AAGAAAGCTC TGAGTGGAGG CGTCACCCTG 
GGCCTGACCA TCCAGCCCAA ATCCGGATCC TCGGGCCTCC AGTCGCCCGC CGTGTCGCGG 
CGGACTCTCG AGTCCTCGCC GTTTTCCAGC TGCGACACCA GCGACACGGA GTCGTGCTCC 
CTGCCCGTCA ACAGCGCCTC GCTGCTCCGC CGGCAGCCGC CCGGCCAGAA GCCCTCGGCG 
GTGCGGGGAC ACGTCCTGAG GAAGGCCAAG GAATGGGAGC AGAAGAACGG CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

afap1 ( XM_011606464.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604766.2 Takifugu rubripes actin filament associated protein 1 (afap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo71203
Clone ID Related Accession (Same CDS sequence)	XM_011606464.2
Accession Version	XM_011606464.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	actin filament-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011606464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGAGCG AGCCCGTTCT CTTCACTTCC AACAGGGCGA TGGAGGAGCT GCTGTGCGAG 
CTTCGTCTCT TCCTGGACCT GTTGGACAGG GAGTACCTGA GTGCCGGGGT CCGAGAGAGG 
AAGCTGCACC TCTCCAACAT CCTCCACAGA GTCCTGTCGG AGAGAGAGCC CTCATTCAAG 
CCGGAGACAC CGAGTGGTCT GCCGGCCCCT CCCCAGATGC CCCTTCCTGA GATTCCTCAG 
CCCTGGCTGC CTCCGAACAG CGGCCCTCCG CCTCTGCCCA GCTCCTCCCT ACCTGAAGGT 
TACTACGAAG AGGCGGTTCC TTTAGGTCCT GGAAAAGCTC CAGAATACAT CACCTCCCAC 
TACGACTCGG ACGCCATGAG CAGCTCCTAC GAGTCCTACG ATGAAGAAGA GGAGGACGGC 
AAAGGTCAGA AGATGCGTCA CCAGTGGCCG TCAGAGGAGG CGTCCATGGA CTTGGTGAAG 
GACGCTCGGA TCTGCGCCTT CCTGCTGCGT AAGAAGCGCT TCGGCCAGTG GACCAAACTG 
CTGTGTGTCA TCAAAGACAA CAAGCTGCTG TGTTACAAGT CCTCCAAAGA CCACACCCCC 
CAGATGGAGC TGATCTTGTC TGGGTGCAGC GTCACGCACG TCCCCAAAGA CGGCAAGAAG 
AAGAAGCACG AGCTGAAGAT CGTCCACCAG GGGGCAGACC CTCTGGTGCT AGCCGTGCAG 
AGCAAAGAGC AGGCCGAGGA GTGGCTCAAG GTGATGAAAG AAGTTTGCGT TAATGGGAAC 
GTGGATCTGG ACCGAGTTGG ATCTGGATCC CCGGTTCACA AACCTGAGCT GGAAAAGAAG 
GCTTCATGTG ACAGGCCCAG CTCTGACGGC GAGCTGACTC ATGAGAACGG ACACAGCGAC 
TGTAAGGACC AAGGAAAAGG AAAGAAGAGC TCCAAGTCGG AGCAGAAGAG CGGGACAGTG 
GGGAAGGGGG CAGGCAAGAC CATCACGAAG ATCATCGGCC TGGGGAAGAA GAAGCCGTCG 
GTGGACGAGC AGACGTCGTC GGCGGAGGAG GACGTGCCCA CGTGTGGTTA CCTTAACGTC 
CTGTCCAACA ACCGCTGGCG GGAGCGCTGG TGCCGCCTGA AGGACAACCA GCTGCTGCTC 
CACAAAGACC GAGACGACCT GAAGAGCCAC ATCGCCTCGC TGCCCCTGCG CGGCTGCGAG 
GTCAGCCCCG GCCTCGACCA CAAGCACCCG TTTGCCTTCC GCCTGCTGCG TAACGGCCAG 
GAGGTGGCGG TGCTGGAGGC GTCCTCCTCA GAGGCGATGG GGCGGTGGTT GGGGGTCTTA 
CTGGCTGAGA CCGGCTCCAC CACTGATCCA GCCACGCTGC ACTACGACTA CATCGACGTG 
GAGACAACAG CCAACATCAT CCAGCTGGCC AAGCAGTCCT TCTGCTTCAC CAGCAAGCGC 
GCTGTGTCTC CAAACCCTTA CCTGGACAAC CCGGTCAACG GCTACGCCTG TCCCACTGGG 
ATGGCCCTGC ACTATGATGA TGTGCCCATT AATGGGACGG TGAAGGGATC AAAGGGATTG 
ACCAACGGGT TGTCGTGGAA ACAGAAGGTG GATCCAAAGA AGCAGGTGAA AAAGAGCGCG 
GTGAACGTGA TGAACGGTAC GGGCTCGCTC AAACGCAACG GCTCCGACGC AGACCAGTGC 
AAGTACGGGA AGAACCGCGT GGAGGCGGAC GCCAGGCGGC TGCAGTGCAA AGAGGAAGAG 
CTGATGAAGA GGAAGCAGGA GATCCGGAGC CACCTGAGCC AGCTGAAGAA GGACAGGAGG 
GACATGAAAG CAGCTCTGGA AACCTCCACA GGAAAACGTT CCCACGCTTT GCTGGCGGAG 
CGGCTGAAGA AGACGGAGGA GGAGTGCAAA CAGAAGGAGG AGGAGTGCGT GAACCTGGAG 
CTGGAGCTGA CCGAGGTGAA GGAGAACCTG AAGAAAGCTC TGAGTGGAGG CGTCACCCTG 
GGCCTGACCA TCCAGCCCAA ATCCGGATCC TCGGGCCTCC AGTCGCCCGC CGTGTCGCGG 
CGGACTCTCG AGTCCTCGCC GTTTTCCAGC TGCGACACCA GCGACACGGA GTCGTGCTCC 
CTGCCCGTCA ACAGCGCCTC GCTGCTCCGC CGGCAGCCGC CCGGCCAGAA GCCCTCGGCG 
GTGCGGGGAC ACGTCCTGAG GAAGGCCAAG GAATGGGAGC AGAAGAACGG CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604766.2
CDS	206..2422
Translation	MRSEPVLFTSNRAMEELLCELRLFLDLLDREYLSAGVRERKLHLSNILHRVLSEREPSFKPETPSGLPAPPQMPLPEIPQPWLPPNSGPPPLPSSSLPEGYYEEAVPLGPGKAPEYITSHYDSDAMSSSYESYDEEEEDGKGQKMRHQWPSEEASMDLVKDARICAFLLRKKRFGQWTKLLCVIKDNKLLCYKSSKDHTPQMELILSGCSVTHVPKDGKKKKHELKIVHQGADPLVLAVQSKEQAEEWLKVMKEVCVNGNVDLDRVGSGSPVHKPELEKKASCDRPSSDGELTHENGHSDCKDQGKGKKSSKSEQKSGTVGKGAGKTITKIIGLGKKKPSVDEQTSSAEEDVPTCGYLNVLSNNRWRERWCRLKDNQLLLHKDRDDLKSHIASLPLRGCEVSPGLDHKHPFAFRLLRNGQEVAVLEASSSEAMGRWLGVLLAETGSTTDPATLHYDYIDVETTANIIQLAKQSFCFTSKRAVSPNPYLDNPVNGYACPTGMALHYDDVPINGTVKGSKGLTNGLSWKQKVDPKKQVKKSAVNVMNGTGSLKRNGSDADQCKYGKNRVEADARRLQCKEEELMKRKQEIRSHLSQLKKDRRDMKAALETSTGKRSHALLAERLKKTEEECKQKEEECVNLELELTEVKENLKKALSGGVTLGLTIQPKSGSSGLQSPAVSRRTLESSPFSSCDTSDTESCSLPVNSASLLRRQPPGQKPSAVRGHVLRKAKEWEQKNGT

Target ORF information:

RefSeq Version	XM_011606464.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes actin filament associated protein 1 (afap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606464.2

ORF Insert Sequence:

ATGCGGAGCG AGCCCGTTCT CTTCACTTCC AACAGGGCGA TGGAGGAGCT GCTGTGCGAG 
CTTCGTCTCT TCCTGGACCT GTTGGACAGG GAGTACCTGA GTGCCGGGGT CCGAGAGAGG 
AAGCTGCACC TCTCCAACAT CCTCCACAGA GTCCTGTCGG AGAGAGAGCC CTCATTCAAG 
CCGGAGACAC CGAGTGGTCT GCCGGCCCCT CCCCAGATGC CCCTTCCTGA GATTCCTCAG 
CCCTGGCTGC CTCCGAACAG CGGCCCTCCG CCTCTGCCCA GCTCCTCCCT ACCTGAAGGT 
TACTACGAAG AGGCGGTTCC TTTAGGTCCT GGAAAAGCTC CAGAATACAT CACCTCCCAC 
TACGACTCGG ACGCCATGAG CAGCTCCTAC GAGTCCTACG ATGAAGAAGA GGAGGACGGC 
AAAGGTCAGA AGATGCGTCA CCAGTGGCCG TCAGAGGAGG CGTCCATGGA CTTGGTGAAG 
GACGCTCGGA TCTGCGCCTT CCTGCTGCGT AAGAAGCGCT TCGGCCAGTG GACCAAACTG 
CTGTGTGTCA TCAAAGACAA CAAGCTGCTG TGTTACAAGT CCTCCAAAGA CCACACCCCC 
CAGATGGAGC TGATCTTGTC TGGGTGCAGC GTCACGCACG TCCCCAAAGA CGGCAAGAAG 
AAGAAGCACG AGCTGAAGAT CGTCCACCAG GGGGCAGACC CTCTGGTGCT AGCCGTGCAG 
AGCAAAGAGC AGGCCGAGGA GTGGCTCAAG GTGATGAAAG AAGTTTGCGT TAATGGGAAC 
GTGGATCTGG ACCGAGTTGG ATCTGGATCC CCGGTTCACA AACCTGAGCT GGAAAAGAAG 
GCTTCATGTG ACAGGCCCAG CTCTGACGGC GAGCTGACTC ATGAGAACGG ACACAGCGAC 
TGTAAGGACC AAGGAAAAGG AAAGAAGAGC TCCAAGTCGG AGCAGAAGAG CGGGACAGTG 
GGGAAGGGGG CAGGCAAGAC CATCACGAAG ATCATCGGCC TGGGGAAGAA GAAGCCGTCG 
GTGGACGAGC AGACGTCGTC GGCGGAGGAG GACGTGCCCA CGTGTGGTTA CCTTAACGTC 
CTGTCCAACA ACCGCTGGCG GGAGCGCTGG TGCCGCCTGA AGGACAACCA GCTGCTGCTC 
CACAAAGACC GAGACGACCT GAAGAGCCAC ATCGCCTCGC TGCCCCTGCG CGGCTGCGAG 
GTCAGCCCCG GCCTCGACCA CAAGCACCCG TTTGCCTTCC GCCTGCTGCG TAACGGCCAG 
GAGGTGGCGG TGCTGGAGGC GTCCTCCTCA GAGGCGATGG GGCGGTGGTT GGGGGTCTTA 
CTGGCTGAGA CCGGCTCCAC CACTGATCCA GCCACGCTGC ACTACGACTA CATCGACGTG 
GAGACAACAG CCAACATCAT CCAGCTGGCC AAGCAGTCCT TCTGCTTCAC CAGCAAGCGC 
GCTGTGTCTC CAAACCCTTA CCTGGACAAC CCGGTCAACG GCTACGCCTG TCCCACTGGG 
ATGGCCCTGC ACTATGATGA TGTGCCCATT AATGGGACGG TGAAGGGATC AAAGGGATTG 
ACCAACGGGT TGTCGTGGAA ACAGAAGGTG GATCCAAAGA AGCAGGTGAA AAAGAGCGCG 
GTGAACGTGA TGAACGGTAC GGGCTCGCTC AAACGCAACG GCTCCGACGC AGACCAGTGC 
AAGTACGGGA AGAACCGCGT GGAGGCGGAC GCCAGGCGGC TGCAGTGCAA AGAGGAAGAG 
CTGATGAAGA GGAAGCAGGA GATCCGGAGC CACCTGAGCC AGCTGAAGAA GGACAGGAGG 
GACATGAAAG CAGCTCTGGA AACCTCCACA GGAAAACGTT CCCACGCTTT GCTGGCGGAG 
CGGCTGAAGA AGACGGAGGA GGAGTGCAAA CAGAAGGAGG AGGAGTGCGT GAACCTGGAG 
CTGGAGCTGA CCGAGGTGAA GGAGAACCTG AAGAAAGCTC TGAGTGGAGG CGTCACCCTG 
GGCCTGACCA TCCAGCCCAA ATCCGGATCC TCGGGCCTCC AGTCGCCCGC CGTGTCGCGG 
CGGACTCTCG AGTCCTCGCC GTTTTCCAGC TGCGACACCA GCGACACGGA GTCGTGCTCC 
CTGCCCGTCA ACAGCGCCTC GCTGCTCCGC CGGCAGCCGC CCGGCCAGAA GCCCTCGGCG 
GTGCGGGGAC ACGTCCTGAG GAAGGCCAAG GAATGGGAGC AGAAGAACGG CACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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afap1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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