HECW2 cDNA ORF clone, Taeniopygia guttata(zebra finch)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW2
Entrez Gene ID	100225246
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Gene Type	protein-coding
Organism	Taeniopygia guttata(zebra finch)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002193323.3	XP_002193359.1	E3 ubiquitin-protein ligase HECW2 isoform X1
XM_030277800.1	XP_030133660.1	E3 ubiquitin-protein ligase HECW2 isoform X2
XM_002193323.2	XP_002193359.1	E3 ubiquitin-protein ligase HECW2

Pathways from BioSystems
Gene Ontology

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTd49913	XM_030277800.1 Latest version!	Taeniopygia guttata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTd11541	XM_002193323.2 Latest version!	Taeniopygia guttata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTd49912	XM_002193323.3 Latest version!	Taeniopygia guttata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW2 ( XM_030277800.1 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_030133660.1 Taeniopygia guttata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTd49913
Clone ID Related Accession (Same CDS sequence)	XM_030277800.1
Accession Version	XM_030277800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	E3 ubiquitin-protein ligase HECW2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044219.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTA GGATCCACAG GCATCCTTTG GAATGGCCAG ATGAGAACTC TCCAGCCAAT 
TTCTGGGATT CCAAGAATCG GGGAGTGACT GGCACACAAA AAGGACAAAT TGTGTGGCGA 
ATTGAACCTG GCCCCTACTT TATGGAATCT GAAATAAAGA TCTGTTTTAA ATACTACCAT 
GGTGTTAGTG GAGCCCTCAG AGCTACTACT CCATGTATCA CTGTGAAAAA CCCTGCTGTG 
ATGATGGGAG TAGAAGGCAC GGATGAAGGT GCTTCTGGAG CTCAGCACTC TAGGAAGTTG 
GTCAGCTTTA CCCTATCAGA TATTAGAGCA GCTGGACTTA AAAAAGGCAT GTTCTTCAAC 
CCTGATCCTT ATCTAAAGAT GTCCATTCAG CCAGGCAAGA AAAGTACATT TCCTACGTTT 
GCTCACCATG GCCAGGAAAG AAGGTCGACT ATCATCAGTA ACACAACTAA TCCTGTTTGG 
CACAGAGAGA AATATTCTTT TGTTGCACTT CTAACAGATG TCCTGGAAAT TGAGGTTAAA 
GACAAATTTG CCAAGAGTCG TCCGATCATC AAGCGCTTCC TTGGGAAGTT AACCATACCT 
GTTCAGAGAC TTTTGGAGAG ACATGCAATT GGAGACCAGG TTCTCAGCTA TAATCTTGGC 
AGAAGGCTCC CAGCTGATCA TGTTAGTGGG TATCTCCAAT TCAAAGTGGA AATCACATCT 
TCTGTTCATG AAGATGCTTC ACCAGAAACA GTTGGGACTT TGCTAGGAAC AAACTCTGTA 
AATGGAGACT TGGGGAGCCC CTCTGATGAT GAGGACATGC CAGCAGGACA TCACGATACA 
TCCACAGTGT GTTCCAATGG CCCAGTCTTT GAGGAATCTT CTGGAGACAC AATCCTGAGA 
CAGTCTTTAA GGACTAGTAG GACTCTGGAG ACAGACCAGG ATGAACTGAC CTCTGGTGCT 
TCAAGATCAT CCCCTCCCAG AGGTCGCCAG GACTCACTAA ATGACTATCT CGATGCAATA 
GAACACAATA ACCATCCCAG GCCAGGGACA TCTGCCTGTG GTGACCGTCC ACGAGGAGCC 
TCCCCAAAAC TGAGGAGTAG CTTCCCTACT GACACAAGAC TTAATGCTAT GCTTCACATT 
GACTCAGATG AAGAGGAGCA CGAGCTGCAT CAAGACCTGG GTTTTCCATC CTCTTTGGAA 
GATGACGGCG GTTTAGTAAT GCTTAATGGT GCTACCAGGA ACGTTGAAAG AAATGAAGTA 
AGTTCCTCAT CAGATCAGGT AATGCCAGGA CCTGCAGAGA GTGAAAGGGT TTCGGCATCA 
GCTGATGACC AGCAAGATTC TCTCCCAGAG CTTCCACCTC CAGAGGAAGG AGCCGAGTGC 
CTGCAGGGCT CTCAGGCTGA AGCCCCGGCA GAGCAGGCCG GCAGCGAGTC CTCTGCCCCT 
CCGGAGGCAG AGCAGCCTCC CGGCAGCGCG GACTCGGGAG CTGCTGAAGC TGCGGGTGGG 
AGCAGAAGGG GCGTCAGCGA AACGGAGTCC ATCGACCAAG GCTCGGAGCC TTCCCAGCTG 
TCGTCAGAGA CGGAGCAGAG CGACCCCGCT CGCACCGAGA GCGTCAGCGA GGCCAGCACC 
CGGCCCGAGG GGGAGAGCGA CGCCGAGGGG GCCGACAGCT CCTGCAACGA GAGCGCGACC 
GTGCGGCGCT CCTCCGTGGA GATGCGCTGC TCCTCCTGCG AGAGCGCCCG GTTCCCCGAG 
ACCCCAGCCT TCTCTCCCCA GGAGGAGGAG GAGGAGGGAG CCTGTGCCCC CGAGGCAGCT 
GCGGCGGAGC CGGGTGCCGA AGCGCAGGAC TCTGCCGGCG CTGCTGGTTC TCTGCCCGCG 
GTACAGTTAC CTCAGGAGGA GGCGCAGGAG GCTGAGGCAG GTGAGCTGCA AGACCAAGAG 
GAAGCAGAAG AAATCTGGCA AAGAAGAAGA AGCCTGCAGG CAGCAGCTGC CCAGAGCCAG 
TCTCAGGATG AAGAAGTTGA GGGAGCACAG GCGGCTTGTG AGGGTGCCAC AGCACAGCTT 
GAAGGAGCTA CTGGAGGTTC TCCTGCCAAT GGCCATCAGC CCTTGAGGTC CCTGCCTTCA 
GTGCGTCAGG ACGTTAGCCG GTACCAGAGA GTGGATGAGG CTCTGCCACC AAACTGGGAG 
GCTCGAATTG ACAGCCACGG ACGGATTTTT TACGTGGACC ACGTGAACAG GACAACAACA 
TGGCAGCGAC CCACAGCTCC CCCAGCCCCA CAGATCCTGC AGAGGTCCAA TTCCATACAG 
CAGATGGAGC AGCTGAATCG CCGGTACCAG AGCATCCGAA GAACAATGAC AAATGACAGG 
CCTGAAGAGC ACACAAATGC CGTGGATGGA GCTGCAGAGG AAGCTGAATT TCACCATTCT 
AATTCAGATT TTCGAAGAGA GAATGTGCTG CCTCACTCTA CCTCCAGATC CAGACTTACT 
TTATTGCTCC AGTCTCCCCC TGTGAAATTC CTTATCAGCC CAGAGTTCTT TACAGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTCTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTC GAGCGCTACC AGCACAACCG GGATCTGGTG 
GGCTTCCTCA ACATGTTTGC CAACAAACAG CTGGAGCTGC CGAGGGGCTG GGAAATGAAA 
CACGACCATC AGGGCAAGGC TTTCTTTGTC GACCACAACT CGCGCACCAC CACGTTCATC 
GACCCGCGGC TGCCCCTGCA GAGCAGCAGG CCCACCAGTG CGCTGGTCCA TCGGCAGCAC 
CTGACCCGGC AGCGCAGCCA CAGTGCTGGG GAGGTCGGAG AGGACTCCCG TCATTCAGGA 
CCCCCAGTTT TGCCAAGACC ATCCAGCACA TTTAACACAG TCAGCAGAGC TCAGTACCAG 
GATGTGGTTC CAGTGGCTTA CAATGACAAG ATAGTTGCAT TTTTGCGGCA GCCCAATATC 
TTTGAAATTC TACAAGAGCG CCAACCAGAT CTTACCAGGA ATCATTCACT CAGGGAGAAG 
ATCCAGTTCA TCCGGACAGA GGGAACACCT GGGCTGGTGC GTTTATCCAG TGATGCAGAC 
CTTGTCATGT TACTCAGCTT GTTTGAGGAG GAAATAATGT CATATGTGCC GCCACATGCC 
TTACTTCATC CTAGTTATTG CCAGTCCCCG CGAGGCTCGC CGGTGTCTTC ACCTCAGAAC 
TCTCCAGGTA CTCAGCGTGC CAATGCCCGA GCTCCAGCTC CGTACAAAAG AGACTTCGAA 
GCCAAGTTGA GGAACTTCTA CAGGAAGTTG GAGACTAAAG GATATGGACA AGGCCCAGGG 
AAATTGAAAT TAATCATCAG GAGAGATCAC TTATTAGAAG ATGCCTTTAA CCAGATCATG 
GGCTACTCGA GAAAAGACCT GCAGAGAAAT AAACTCTATG TCACGTTTGT TGGGGAAGAA 
GGGTTGGACT ACAGTGGACC ATCAAGAGAG TTTTTTTTCC TGGTGTCCAG GGAGCTCTTC 
AATCCATATT ATGGTTTGTT TGAATATTCA GCCAACGATA CCTACACAGT ACAAATAAGT 
CCCATGTCTG CTTTCGTAGA CAATCACCAT GAGTGGTTCA GGTTTAGTGG TCGAATTCTT 
GGTCTTGCTC TAATACATCA GTATTTGCTG GATGCCTTTT TTACAAGACC CTTTTATAAA 
GCCCTCCTCC GAATACTCTG TGACCTGAGT GACTTAGAAT ATCTGGATGA GGAGTTTCAC 
CAGAGTTTGC AGTGGATGAA AGACAATGAC ATACACGATA TCCTAGATCT CACATTTACT 
GTAAATGAAG AAGTTTTTGG GCAGATCACA GAACGGGAAC TAAAACCAGG AGGTGCCAAT 
ATTCCAGTCA CTGAAAAGAA CAAGAAAGAA TATATCGAGA GAATGGTGAA ATGGAGAATT 
GAGAGAGGAG TTGTACAACA AACAGAAAGT CTGGTTCGTG GTTTCTATGA GGTGGTTGAT 
GCAAGGCTGG TGTCTGTATT TGATGCCAGA GAACTGGAAC TGGTTATAGC TGGAACAGCA 
GAAATTGACT TGAGTGATTG GAGAAATAAT ACAGAGTACA GAGGAGGTTA TCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCT GTGGAAAGGT TCAACAATGA ACAACGTCTA 
AGACTTTTAC AGTTTGTCAC AGGCACGTCC AGCATTCCCT ACGAAGGCTT TGCCTCTCTC 
CGGGGCAGCA ACGGGCCCAG GCGCTTCTGC GTGGAGAAGT GGGGGAAGAT CACTGCTCTT 
CCAAGGGCTC ACACTTGTTT CAATCGTCTG GACCTTCCCC CTTACCCATC ATTCTCTATG 
TTATATGAAA AACTGTTGAC AGCAGTAGAA GAGACAAGTA CTTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030133660.1
CDS	94..4530
Translation	MQFRIHRHPLEWPDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMESEIKICFKYYHGVSGALRATTPCITVKNPAVMMGVEGTDEGASGAQHSRKLVSFTLSDIRAAGLKKGMFFNPDPYLKMSIQPGKKSTFPTFAHHGQERRSTIISNTTNPVWHREKYSFVALLTDVLEIEVKDKFAKSRPIIKRFLGKLTIPVQRLLERHAIGDQVLSYNLGRRLPADHVSGYLQFKVEITSSVHEDASPETVGTLLGTNSVNGDLGSPSDDEDMPAGHHDTSTVCSNGPVFEESSGDTILRQSLRTSRTLETDQDELTSGASRSSPPRGRQDSLNDYLDAIEHNNHPRPGTSACGDRPRGASPKLRSSFPTDTRLNAMLHIDSDEEEHELHQDLGFPSSLEDDGGLVMLNGATRNVERNEVSSSSDQVMPGPAESERVSASADDQQDSLPELPPPEEGAECLQGSQAEAPAEQAGSESSAPPEAEQPPGSADSGAAEAAGGSRRGVSETESIDQGSEPSQLSSETEQSDPARTESVSEASTRPEGESDAEGADSSCNESATVRRSSVEMRCSSCESARFPETPAFSPQEEEEEGACAPEAAAAEPGAEAQDSAGAAGSLPAVQLPQEEAQEAEAGELQDQEEAEEIWQRRRSLQAAAAQSQSQDEEVEGAQAACEGATAQLEGATGGSPANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNDRPEEHTNAVDGAAEEAEFHHSNSDFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRAQYQDVVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_030277800.1
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030277800.1

ORF Insert Sequence:

ATGCAGTTTA GGATCCACAG GCATCCTTTG GAATGGCCAG ATGAGAACTC TCCAGCCAAT 
TTCTGGGATT CCAAGAATCG GGGAGTGACT GGCACACAAA AAGGACAAAT TGTGTGGCGA 
ATTGAACCTG GCCCCTACTT TATGGAATCT GAAATAAAGA TCTGTTTTAA ATACTACCAT 
GGTGTTAGTG GAGCCCTCAG AGCTACTACT CCATGTATCA CTGTGAAAAA CCCTGCTGTG 
ATGATGGGAG TAGAAGGCAC GGATGAAGGT GCTTCTGGAG CTCAGCACTC TAGGAAGTTG 
GTCAGCTTTA CCCTATCAGA TATTAGAGCA GCTGGACTTA AAAAAGGCAT GTTCTTCAAC 
CCTGATCCTT ATCTAAAGAT GTCCATTCAG CCAGGCAAGA AAAGTACATT TCCTACGTTT 
GCTCACCATG GCCAGGAAAG AAGGTCGACT ATCATCAGTA ACACAACTAA TCCTGTTTGG 
CACAGAGAGA AATATTCTTT TGTTGCACTT CTAACAGATG TCCTGGAAAT TGAGGTTAAA 
GACAAATTTG CCAAGAGTCG TCCGATCATC AAGCGCTTCC TTGGGAAGTT AACCATACCT 
GTTCAGAGAC TTTTGGAGAG ACATGCAATT GGAGACCAGG TTCTCAGCTA TAATCTTGGC 
AGAAGGCTCC CAGCTGATCA TGTTAGTGGG TATCTCCAAT TCAAAGTGGA AATCACATCT 
TCTGTTCATG AAGATGCTTC ACCAGAAACA GTTGGGACTT TGCTAGGAAC AAACTCTGTA 
AATGGAGACT TGGGGAGCCC CTCTGATGAT GAGGACATGC CAGCAGGACA TCACGATACA 
TCCACAGTGT GTTCCAATGG CCCAGTCTTT GAGGAATCTT CTGGAGACAC AATCCTGAGA 
CAGTCTTTAA GGACTAGTAG GACTCTGGAG ACAGACCAGG ATGAACTGAC CTCTGGTGCT 
TCAAGATCAT CCCCTCCCAG AGGTCGCCAG GACTCACTAA ATGACTATCT CGATGCAATA 
GAACACAATA ACCATCCCAG GCCAGGGACA TCTGCCTGTG GTGACCGTCC ACGAGGAGCC 
TCCCCAAAAC TGAGGAGTAG CTTCCCTACT GACACAAGAC TTAATGCTAT GCTTCACATT 
GACTCAGATG AAGAGGAGCA CGAGCTGCAT CAAGACCTGG GTTTTCCATC CTCTTTGGAA 
GATGACGGCG GTTTAGTAAT GCTTAATGGT GCTACCAGGA ACGTTGAAAG AAATGAAGTA 
AGTTCCTCAT CAGATCAGGT AATGCCAGGA CCTGCAGAGA GTGAAAGGGT TTCGGCATCA 
GCTGATGACC AGCAAGATTC TCTCCCAGAG CTTCCACCTC CAGAGGAAGG AGCCGAGTGC 
CTGCAGGGCT CTCAGGCTGA AGCCCCGGCA GAGCAGGCCG GCAGCGAGTC CTCTGCCCCT 
CCGGAGGCAG AGCAGCCTCC CGGCAGCGCG GACTCGGGAG CTGCTGAAGC TGCGGGTGGG 
AGCAGAAGGG GCGTCAGCGA AACGGAGTCC ATCGACCAAG GCTCGGAGCC TTCCCAGCTG 
TCGTCAGAGA CGGAGCAGAG CGACCCCGCT CGCACCGAGA GCGTCAGCGA GGCCAGCACC 
CGGCCCGAGG GGGAGAGCGA CGCCGAGGGG GCCGACAGCT CCTGCAACGA GAGCGCGACC 
GTGCGGCGCT CCTCCGTGGA GATGCGCTGC TCCTCCTGCG AGAGCGCCCG GTTCCCCGAG 
ACCCCAGCCT TCTCTCCCCA GGAGGAGGAG GAGGAGGGAG CCTGTGCCCC CGAGGCAGCT 
GCGGCGGAGC CGGGTGCCGA AGCGCAGGAC TCTGCCGGCG CTGCTGGTTC TCTGCCCGCG 
GTACAGTTAC CTCAGGAGGA GGCGCAGGAG GCTGAGGCAG GTGAGCTGCA AGACCAAGAG 
GAAGCAGAAG AAATCTGGCA AAGAAGAAGA AGCCTGCAGG CAGCAGCTGC CCAGAGCCAG 
TCTCAGGATG AAGAAGTTGA GGGAGCACAG GCGGCTTGTG AGGGTGCCAC AGCACAGCTT 
GAAGGAGCTA CTGGAGGTTC TCCTGCCAAT GGCCATCAGC CCTTGAGGTC CCTGCCTTCA 
GTGCGTCAGG ACGTTAGCCG GTACCAGAGA GTGGATGAGG CTCTGCCACC AAACTGGGAG 
GCTCGAATTG ACAGCCACGG ACGGATTTTT TACGTGGACC ACGTGAACAG GACAACAACA 
TGGCAGCGAC CCACAGCTCC CCCAGCCCCA CAGATCCTGC AGAGGTCCAA TTCCATACAG 
CAGATGGAGC AGCTGAATCG CCGGTACCAG AGCATCCGAA GAACAATGAC AAATGACAGG 
CCTGAAGAGC ACACAAATGC CGTGGATGGA GCTGCAGAGG AAGCTGAATT TCACCATTCT 
AATTCAGATT TTCGAAGAGA GAATGTGCTG CCTCACTCTA CCTCCAGATC CAGACTTACT 
TTATTGCTCC AGTCTCCCCC TGTGAAATTC CTTATCAGCC CAGAGTTCTT TACAGTGCTG 
CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTCTGAA GCACATGATC 
ACCAAAGTCC GGCGGGACAC CCACCACTTC GAGCGCTACC AGCACAACCG GGATCTGGTG 
GGCTTCCTCA ACATGTTTGC CAACAAACAG CTGGAGCTGC CGAGGGGCTG GGAAATGAAA 
CACGACCATC AGGGCAAGGC TTTCTTTGTC GACCACAACT CGCGCACCAC CACGTTCATC 
GACCCGCGGC TGCCCCTGCA GAGCAGCAGG CCCACCAGTG CGCTGGTCCA TCGGCAGCAC 
CTGACCCGGC AGCGCAGCCA CAGTGCTGGG GAGGTCGGAG AGGACTCCCG TCATTCAGGA 
CCCCCAGTTT TGCCAAGACC ATCCAGCACA TTTAACACAG TCAGCAGAGC TCAGTACCAG 
GATGTGGTTC CAGTGGCTTA CAATGACAAG ATAGTTGCAT TTTTGCGGCA GCCCAATATC 
TTTGAAATTC TACAAGAGCG CCAACCAGAT CTTACCAGGA ATCATTCACT CAGGGAGAAG 
ATCCAGTTCA TCCGGACAGA GGGAACACCT GGGCTGGTGC GTTTATCCAG TGATGCAGAC 
CTTGTCATGT TACTCAGCTT GTTTGAGGAG GAAATAATGT CATATGTGCC GCCACATGCC 
TTACTTCATC CTAGTTATTG CCAGTCCCCG CGAGGCTCGC CGGTGTCTTC ACCTCAGAAC 
TCTCCAGGTA CTCAGCGTGC CAATGCCCGA GCTCCAGCTC CGTACAAAAG AGACTTCGAA 
GCCAAGTTGA GGAACTTCTA CAGGAAGTTG GAGACTAAAG GATATGGACA AGGCCCAGGG 
AAATTGAAAT TAATCATCAG GAGAGATCAC TTATTAGAAG ATGCCTTTAA CCAGATCATG 
GGCTACTCGA GAAAAGACCT GCAGAGAAAT AAACTCTATG TCACGTTTGT TGGGGAAGAA 
GGGTTGGACT ACAGTGGACC ATCAAGAGAG TTTTTTTTCC TGGTGTCCAG GGAGCTCTTC 
AATCCATATT ATGGTTTGTT TGAATATTCA GCCAACGATA CCTACACAGT ACAAATAAGT 
CCCATGTCTG CTTTCGTAGA CAATCACCAT GAGTGGTTCA GGTTTAGTGG TCGAATTCTT 
GGTCTTGCTC TAATACATCA GTATTTGCTG GATGCCTTTT TTACAAGACC CTTTTATAAA 
GCCCTCCTCC GAATACTCTG TGACCTGAGT GACTTAGAAT ATCTGGATGA GGAGTTTCAC 
CAGAGTTTGC AGTGGATGAA AGACAATGAC ATACACGATA TCCTAGATCT CACATTTACT 
GTAAATGAAG AAGTTTTTGG GCAGATCACA GAACGGGAAC TAAAACCAGG AGGTGCCAAT 
ATTCCAGTCA CTGAAAAGAA CAAGAAAGAA TATATCGAGA GAATGGTGAA ATGGAGAATT 
GAGAGAGGAG TTGTACAACA AACAGAAAGT CTGGTTCGTG GTTTCTATGA GGTGGTTGAT 
GCAAGGCTGG TGTCTGTATT TGATGCCAGA GAACTGGAAC TGGTTATAGC TGGAACAGCA 
GAAATTGACT TGAGTGATTG GAGAAATAAT ACAGAGTACA GAGGAGGTTA TCATGACAAT 
CACATTGTAA TTCGGTGGTT CTGGGCTGCT GTGGAAAGGT TCAACAATGA ACAACGTCTA 
AGACTTTTAC AGTTTGTCAC AGGCACGTCC AGCATTCCCT ACGAAGGCTT TGCCTCTCTC 
CGGGGCAGCA ACGGGCCCAG GCGCTTCTGC GTGGAGAAGT GGGGGAAGAT CACTGCTCTT 
CCAAGGGCTC ACACTTGTTT CAATCGTCTG GACCTTCCCC CTTACCCATC ATTCTCTATG 
TTATATGAAA AACTGTTGAC AGCAGTAGAA GAGACAAGTA CTTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_002193323.2 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_002193359.1 Taeniopygia guttata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Price & Availability↑

CloneID	OTd11541
Clone ID Related Accession (Same CDS sequence)	XM_002193323.2
Accession Version	XM_002193323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1432483200000
Organism	Taeniopygia guttata(zebra finch)
Product	E3 ubiquitin-protein ligase HECW2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002198919.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_002193323.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Taeniopygia guttata Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CAGCCCGAGA ACACCTGCTC TTCGTGCGCC GTCGCAACCC GCAGCTCCGG 
TACACCCTGA GCCCCGAGAA CCTGCAGAGT TTGGCCTCCC AGGGCACCAT GCCGGAGAAC 
ATGAACTTGC AGCGTGCCAA CAGTGACACT GATTTAGTGA CCTCGGATAG CAGGTCTAGT 
TTGACAGCCA GCATGTATGA ATACACCTTG GGACAGTCTC AGAACCTCAT CATTTTCTGG 
GATATTAAGG AAGAAGTAGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC 
TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA 
ATTGTGTGGC GAATTGAACC TGGCCCCTAC TTTATGGAAT CTGAAATAAA GATCTGTTTT 
AAATACTACC ATGGTGTTAG TGGAGCCCTC AGAGCTACTA CTCCATGTAT CACTGTGAAA 
AACCCTGCTG TGATGATGGG AGTAGAAGGC ACGGATGAAG GTGCTTCTGG AGCTCAGCAC 
TCTAGGAAGT TGGTCAGCTT TACCCTATCA GATATTAGAG CAGCTGGACT TAAAAAAGGC 
ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATTC AGCCAGGCAA GAAAAGTACA 
TTTCCTACGT TTGCTCACCA TGGCCAGGAA AGAAGGTCGA CTATCATCAG TAACACAACT 
AATCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA 
ATTGAGGTTA AAGACAAATT TGCCAAGAGT CGTCCGATCA TCAAGCGCTT CCTTGGGAAG 
TTAACCATAC CTGTTCAGAG ACTTTTGGAG AGACATGCAA TTGGAGACCA GGTTCTCAGC 
TATAATCTTG GCAGAAGGCT CCCAGCTGAT CATGTTAGTG GGTATCTCCA ATTCAAAGTG 
GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGGAC TTTGCTAGGA 
ACAAACTCTG TAAATGGAGA CTTGGGGAGC CCCTCTGATG ATGAGGACAT GCCAGCAGGA 
CATCACGATA CGTCCACGGT GTGTTCCAAT GGCCCAGTCT TTGAGGAATC TTCTGGAGAC 
ACAATCCTGA GACAGTCTTT AAGGACTAGT AGGACTCTGG AGACAGACCA GGATGAACTG 
ACCTCTGGTG CTTCAAGATC ATCCCCTCCC AGAGGTCGCC AGGACTCACT AAATGACTAT 
CTCGATGCAA TAGAACACAA TAACCATCCC AGGCCAGGGA CATCTGCCTG TGGTGACCGT 
CCACGAGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCT 
ATGCTTCACA TTGACTCAGA TGAAGAGGAG CACGAGCTGC ATCAAGACCT GGGTTTTCCA 
TCCTCTTTGG AAGATGACGG CGGTTTAGTA ATGCTTAATG GTGCTACCAG GAACGTTGAA 
AGAAATGAAG TAAGTTCCTC ATCAGATCAG GTAATGCCAG GACCTGCAGA GAGTGAAAGG 
GTTTCGGCAT CAGCTGATGA CCAGCAAGAT TCTCTCCCAG AGCTTCCACC TCCAGAGGAA 
GGAGCCGAGT GCCTGCAGGG CTCTCAGGCT GAAGCCCCGG CAGAGCAGGC CGGCAGCGAG 
TCCTCTGCCC CTCCGGAGGC AGAGCAGCCT CCCGGCAGCG CGGACTCGGG AGCTGCTGAA 
GCTGCGGGTG GGAGCAGAAG GGGCGTCAGC GAAACGGAGT CCATCGACCA AGGCTCGGAG 
CCTTCCCAGC TGTCGTCAGA GACGGAGCAG AGCGACCCCG CTCGCACCGA GAGCGTCAGC 
GAGGCCAGCA CCCGGCCCGA GGGGGAGAGC GACGCCGAGG GGGCCGACAG CTCCTGCAAC 
GAGAGCGCGA CCGTGCGGCG CTCCTCCGTG GAGATGCGCT GCTCCTCCTG CGAGAGCGCC 
CGGTTCCCCG AGACCCCAGC CTTCTCTCCC CAGGAGGAGG AGGAGGAGGG AGCCTGTGCC 
CCCGAGGCAG CTGCGGCGGA GCCGGGTGCC GAAGCGCAGG ACTCTGCCGG CGCTGCTGGT 
TCTCTGCCCG CGGTACAGTT ACCTCAGGAG GAGGCGCAGG AGGCTGAGGC AGGTGAGCTG 
CAAGACCAAG AGGAAGCAGA AGAAATCTGG CAAAGAAGAA GAAGCCTGCA GGCAGCAGCT 
GCCCAGAGCC AGTCTCAGGA TGAAGAAGTT GAGGGAGCAC AGGCGGCTTG TGAGGGTGCC 
ACAGCACAGC TTGAAGGAGC TACTGGAGGT TCTCCTGCCA ATGGCCATCA GCCCTTGAGG 
TCCCTGCCTT CAGTGCGTCA GGACGTTAGC CGGTACCAGA GAGTGGATGA GGCTCTGCCA 
CCAAACTGGG AGGCTCGAAT TGACAGCCAC GGACGGATTT TTTACGTGGA CCACGTGAAC 
AGGACAACAA CATGGCAGCG ACCCACAGCT CCCCCAGCCC CACAGATCCT GCAGAGGTCC 
AATTCCATAC AGCAGATGGA GCAGCTGAAT CGCCGGTACC AGAGCATCCG AAGAACAATG 
ACAAATGACA GGCCTGAAGA GCACACAAAT GCCGTGGATG GAGCTGCAGA GGAAGCTGAA 
TTTCACCATT CTAATTCAGA TTTTCGAAGA GAGAATGTGC TGCCTCACTC TACCTCCAGA 
TCCAGACTTA CTTTATTGCT CCAGTCTCCC CCTGTGAAAT TCCTTATCAG CCCAGAGTTC 
TTTACAGTGC TGCATTCTAA CCCTAGTGCC TACCGCATGT TTACAAACAA CACGTGTCTG 
AAGCACATGA TCACCAAAGT CCGGCGGGAC ACCCACCACT TCGAGCGCTA CCAGCACAAC 
CGGGATCTGG TGGGCTTCCT CAACATGTTT GCCAACAAAC AGCTGGAGCT GCCGAGGGGC 
TGGGAAATGA AACACGACCA TCAGGGCAAG GCTTTCTTTG TCGACCACAA CTCGCGCACC 
ACCACGTTCA TCGACCCGCG GCTGCCCCTG CAGAGCAGCA GGCCCACCAG TGCGCTGGTC 
CATCGGCAGC ACCTGACCCG GCAGCGCAGC CACAGTGCTG GGGAGGTCGG AGAGGACTCC 
CGTCATTCAG GACCCCCAGT TTTGCCAAGA CCATCCAGCA CATTTAACAC AGTCAGCAGA 
GCTCAGTACC AGGATGTGGT TCCAGTGGCT TACAATGACA AGATAGTTGC ATTTTTGCGG 
CAGCCCAATA TCTTTGAAAT TCTACAAGAG CGCCAACCAG ATCTTACCAG GAATCATTCA 
CTCAGGGAGA AGATCCAGTT CATCCGGACA GAGGGAACAC CTGGGCTGGT GCGTTTATCC 
AGTGATGCAG ACCTTGTCAT GTTACTCAGC TTGTTTGAGG AGGAAATAAT GTCATATGTG 
CCGCCACATG CCTTACTTCA TCCTAGTTAT TGCCAGTCCC CGCGAGGCTC GCCGGTGTCT 
TCACCTCAGA ACTCTCCAGG TACTCAGCGT GCCAATGCCC GAGCTCCAGC TCCGTACAAA 
AGAGACTTCG AAGCCAAGTT GAGGAACTTC TACAGGAAGT TGGAGACTAA AGGATATGGA 
CAAGGCCCAG GGAAATTGAA ATTAATCATC AGGAGAGATC ACTTATTAGA AGATGCCTTT 
AACCAGATCA TGGGCTACTC GAGAAAAGAC CTGCAGAGAA ATAAACTCTA TGTCACGTTT 
GTTGGGGAAG AAGGGTTGGA CTACAGTGGA CCATCAAGAG AGTTTTTTTT CCTGGTGTCC 
AGGGAGCTCT TCAATCCATA TTATGGTTTG TTTGAATATT CAGCCAACGA TACCTACACA 
GTACAAATAA GTCCCATGTC TGCTTTCGTA GACAATCACC ATGAGTGGTT CAGGTTTAGT 
GGTCGAATTC TTGGTCTTGC TCTAATACAT CAGTATTTGC TGGATGCCTT TTTTACAAGA 
CCCTTTTATA AAGCCCTCCT CCGAATACTC TGTGACCTGA GTGACTTAGA ATATCTGGAT 
GAGGAGTTTC ACCAGAGTTT GCAGTGGATG AAAGACAATG ACATACACGA TATCCTAGAT 
CTCACATTTA CCGTAAATGA AGAAGTTTTT GGGCAGATCA CAGAACGGGA ACTAAAACCA 
GGAGGTGCCA ATATTCCAGT CACTGAAAAG AACAAGAAAG AATATATCGA GAGAATGGTG 
AAATGGAGAA TTGAGAGAGG AGTTGTACAA CAAACAGAAA GTCTGGTTCG TGGTTTCTAT 
GAGGTGGTTG ATGCAAGGCT GGTGTCTGTA TTTGATGCCA GAGAACTGGA ACTGGTTATA 
GCTGGAACAG CAGAAATTGA CTTGAGTGAT TGGAGAAATA ATACAGAGTA CAGAGGAGGT 
TATCATGACA ATCACATTGT AATTCGGTGG TTCTGGGCTG CTGTGGAAAG GTTCAACAAT 
GAACAACGTC TAAGACTTTT ACAGTTTGTC ACAGGCACGT CCAGCATTCC CTACGAAGGC 
TTTGCCTCTC TCCGGGGCAG CAACGGGCCC AGGCGCTTCT GCGTGGAGAA GTGGGGGAAG 
ATCACTGCTC TTCCAAGGGC TCACACTTGT TTCAATCGTC TGGACCTTCC CCCTTACCCA 
TCATTCTCTA TGTTATATGA AAAACTGTTG ACAGCAGTAG AAGAGACAAG TACTTTTGGA 
CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002193359.1
CDS	100..4788
Translation	MASSAREHLLFVRRRNPQLRYTLSPENLQSLASQGTMPENMNLQRANSDTDLVTSDSRSSLTASMYEYTLGQSQNLIIFWDIKEEVDPTDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMESEIKICFKYYHGVSGALRATTPCITVKNPAVMMGVEGTDEGASGAQHSRKLVSFTLSDIRAAGLKKGMFFNPDPYLKMSIQPGKKSTFPTFAHHGQERRSTIISNTTNPVWHREKYSFVALLTDVLEIEVKDKFAKSRPIIKRFLGKLTIPVQRLLERHAIGDQVLSYNLGRRLPADHVSGYLQFKVEITSSVHEDASPETVGTLLGTNSVNGDLGSPSDDEDMPAGHHDTSTVCSNGPVFEESSGDTILRQSLRTSRTLETDQDELTSGASRSSPPRGRQDSLNDYLDAIEHNNHPRPGTSACGDRPRGASPKLRSSFPTDTRLNAMLHIDSDEEEHELHQDLGFPSSLEDDGGLVMLNGATRNVERNEVSSSSDQVMPGPAESERVSASADDQQDSLPELPPPEEGAECLQGSQAEAPAEQAGSESSAPPEAEQPPGSADSGAAEAAGGSRRGVSETESIDQGSEPSQLSSETEQSDPARTESVSEASTRPEGESDAEGADSSCNESATVRRSSVEMRCSSCESARFPETPAFSPQEEEEEGACAPEAAAAEPGAEAQDSAGAAGSLPAVQLPQEEAQEAEAGELQDQEEAEEIWQRRRSLQAAAAQSQSQDEEVEGAQAACEGATAQLEGATGGSPANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNDRPEEHTNAVDGAAEEAEFHHSNSDFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRAQYQDVVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_002193323.2
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002193323.2

ORF Insert Sequence:

ATGGCCAGCT CAGCCCGAGA ACACCTGCTC TTCGTGCGCC GTCGCAACCC GCAGCTCCGG 
TACACCCTGA GCCCCGAGAA CCTGCAGAGT TTGGCCTCCC AGGGCACCAT GCCGGAGAAC 
ATGAACTTGC AGCGTGCCAA CAGTGACACT GATTTAGTGA CCTCGGATAG CAGGTCTAGT 
TTGACAGCCA GCATGTATGA ATACACCTTG GGACAGTCTC AGAACCTCAT CATTTTCTGG 
GATATTAAGG AAGAAGTAGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC 
TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA 
ATTGTGTGGC GAATTGAACC TGGCCCCTAC TTTATGGAAT CTGAAATAAA GATCTGTTTT 
AAATACTACC ATGGTGTTAG TGGAGCCCTC AGAGCTACTA CTCCATGTAT CACTGTGAAA 
AACCCTGCTG TGATGATGGG AGTAGAAGGC ACGGATGAAG GTGCTTCTGG AGCTCAGCAC 
TCTAGGAAGT TGGTCAGCTT TACCCTATCA GATATTAGAG CAGCTGGACT TAAAAAAGGC 
ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATTC AGCCAGGCAA GAAAAGTACA 
TTTCCTACGT TTGCTCACCA TGGCCAGGAA AGAAGGTCGA CTATCATCAG TAACACAACT 
AATCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA 
ATTGAGGTTA AAGACAAATT TGCCAAGAGT CGTCCGATCA TCAAGCGCTT CCTTGGGAAG 
TTAACCATAC CTGTTCAGAG ACTTTTGGAG AGACATGCAA TTGGAGACCA GGTTCTCAGC 
TATAATCTTG GCAGAAGGCT CCCAGCTGAT CATGTTAGTG GGTATCTCCA ATTCAAAGTG 
GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGGAC TTTGCTAGGA 
ACAAACTCTG TAAATGGAGA CTTGGGGAGC CCCTCTGATG ATGAGGACAT GCCAGCAGGA 
CATCACGATA CGTCCACGGT GTGTTCCAAT GGCCCAGTCT TTGAGGAATC TTCTGGAGAC 
ACAATCCTGA GACAGTCTTT AAGGACTAGT AGGACTCTGG AGACAGACCA GGATGAACTG 
ACCTCTGGTG CTTCAAGATC ATCCCCTCCC AGAGGTCGCC AGGACTCACT AAATGACTAT 
CTCGATGCAA TAGAACACAA TAACCATCCC AGGCCAGGGA CATCTGCCTG TGGTGACCGT 
CCACGAGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCT 
ATGCTTCACA TTGACTCAGA TGAAGAGGAG CACGAGCTGC ATCAAGACCT GGGTTTTCCA 
TCCTCTTTGG AAGATGACGG CGGTTTAGTA ATGCTTAATG GTGCTACCAG GAACGTTGAA 
AGAAATGAAG TAAGTTCCTC ATCAGATCAG GTAATGCCAG GACCTGCAGA GAGTGAAAGG 
GTTTCGGCAT CAGCTGATGA CCAGCAAGAT TCTCTCCCAG AGCTTCCACC TCCAGAGGAA 
GGAGCCGAGT GCCTGCAGGG CTCTCAGGCT GAAGCCCCGG CAGAGCAGGC CGGCAGCGAG 
TCCTCTGCCC CTCCGGAGGC AGAGCAGCCT CCCGGCAGCG CGGACTCGGG AGCTGCTGAA 
GCTGCGGGTG GGAGCAGAAG GGGCGTCAGC GAAACGGAGT CCATCGACCA AGGCTCGGAG 
CCTTCCCAGC TGTCGTCAGA GACGGAGCAG AGCGACCCCG CTCGCACCGA GAGCGTCAGC 
GAGGCCAGCA CCCGGCCCGA GGGGGAGAGC GACGCCGAGG GGGCCGACAG CTCCTGCAAC 
GAGAGCGCGA CCGTGCGGCG CTCCTCCGTG GAGATGCGCT GCTCCTCCTG CGAGAGCGCC 
CGGTTCCCCG AGACCCCAGC CTTCTCTCCC CAGGAGGAGG AGGAGGAGGG AGCCTGTGCC 
CCCGAGGCAG CTGCGGCGGA GCCGGGTGCC GAAGCGCAGG ACTCTGCCGG CGCTGCTGGT 
TCTCTGCCCG CGGTACAGTT ACCTCAGGAG GAGGCGCAGG AGGCTGAGGC AGGTGAGCTG 
CAAGACCAAG AGGAAGCAGA AGAAATCTGG CAAAGAAGAA GAAGCCTGCA GGCAGCAGCT 
GCCCAGAGCC AGTCTCAGGA TGAAGAAGTT GAGGGAGCAC AGGCGGCTTG TGAGGGTGCC 
ACAGCACAGC TTGAAGGAGC TACTGGAGGT TCTCCTGCCA ATGGCCATCA GCCCTTGAGG 
TCCCTGCCTT CAGTGCGTCA GGACGTTAGC CGGTACCAGA GAGTGGATGA GGCTCTGCCA 
CCAAACTGGG AGGCTCGAAT TGACAGCCAC GGACGGATTT TTTACGTGGA CCACGTGAAC 
AGGACAACAA CATGGCAGCG ACCCACAGCT CCCCCAGCCC CACAGATCCT GCAGAGGTCC 
AATTCCATAC AGCAGATGGA GCAGCTGAAT CGCCGGTACC AGAGCATCCG AAGAACAATG 
ACAAATGACA GGCCTGAAGA GCACACAAAT GCCGTGGATG GAGCTGCAGA GGAAGCTGAA 
TTTCACCATT CTAATTCAGA TTTTCGAAGA GAGAATGTGC TGCCTCACTC TACCTCCAGA 
TCCAGACTTA CTTTATTGCT CCAGTCTCCC CCTGTGAAAT TCCTTATCAG CCCAGAGTTC 
TTTACAGTGC TGCATTCTAA CCCTAGTGCC TACCGCATGT TTACAAACAA CACGTGTCTG 
AAGCACATGA TCACCAAAGT CCGGCGGGAC ACCCACCACT TCGAGCGCTA CCAGCACAAC 
CGGGATCTGG TGGGCTTCCT CAACATGTTT GCCAACAAAC AGCTGGAGCT GCCGAGGGGC 
TGGGAAATGA AACACGACCA TCAGGGCAAG GCTTTCTTTG TCGACCACAA CTCGCGCACC 
ACCACGTTCA TCGACCCGCG GCTGCCCCTG CAGAGCAGCA GGCCCACCAG TGCGCTGGTC 
CATCGGCAGC ACCTGACCCG GCAGCGCAGC CACAGTGCTG GGGAGGTCGG AGAGGACTCC 
CGTCATTCAG GACCCCCAGT TTTGCCAAGA CCATCCAGCA CATTTAACAC AGTCAGCAGA 
GCTCAGTACC AGGATGTGGT TCCAGTGGCT TACAATGACA AGATAGTTGC ATTTTTGCGG 
CAGCCCAATA TCTTTGAAAT TCTACAAGAG CGCCAACCAG ATCTTACCAG GAATCATTCA 
CTCAGGGAGA AGATCCAGTT CATCCGGACA GAGGGAACAC CTGGGCTGGT GCGTTTATCC 
AGTGATGCAG ACCTTGTCAT GTTACTCAGC TTGTTTGAGG AGGAAATAAT GTCATATGTG 
CCGCCACATG CCTTACTTCA TCCTAGTTAT TGCCAGTCCC CGCGAGGCTC GCCGGTGTCT 
TCACCTCAGA ACTCTCCAGG TACTCAGCGT GCCAATGCCC GAGCTCCAGC TCCGTACAAA 
AGAGACTTCG AAGCCAAGTT GAGGAACTTC TACAGGAAGT TGGAGACTAA AGGATATGGA 
CAAGGCCCAG GGAAATTGAA ATTAATCATC AGGAGAGATC ACTTATTAGA AGATGCCTTT 
AACCAGATCA TGGGCTACTC GAGAAAAGAC CTGCAGAGAA ATAAACTCTA TGTCACGTTT 
GTTGGGGAAG AAGGGTTGGA CTACAGTGGA CCATCAAGAG AGTTTTTTTT CCTGGTGTCC 
AGGGAGCTCT TCAATCCATA TTATGGTTTG TTTGAATATT CAGCCAACGA TACCTACACA 
GTACAAATAA GTCCCATGTC TGCTTTCGTA GACAATCACC ATGAGTGGTT CAGGTTTAGT 
GGTCGAATTC TTGGTCTTGC TCTAATACAT CAGTATTTGC TGGATGCCTT TTTTACAAGA 
CCCTTTTATA AAGCCCTCCT CCGAATACTC TGTGACCTGA GTGACTTAGA ATATCTGGAT 
GAGGAGTTTC ACCAGAGTTT GCAGTGGATG AAAGACAATG ACATACACGA TATCCTAGAT 
CTCACATTTA CCGTAAATGA AGAAGTTTTT GGGCAGATCA CAGAACGGGA ACTAAAACCA 
GGAGGTGCCA ATATTCCAGT CACTGAAAAG AACAAGAAAG AATATATCGA GAGAATGGTG 
AAATGGAGAA TTGAGAGAGG AGTTGTACAA CAAACAGAAA GTCTGGTTCG TGGTTTCTAT 
GAGGTGGTTG ATGCAAGGCT GGTGTCTGTA TTTGATGCCA GAGAACTGGA ACTGGTTATA 
GCTGGAACAG CAGAAATTGA CTTGAGTGAT TGGAGAAATA ATACAGAGTA CAGAGGAGGT 
TATCATGACA ATCACATTGT AATTCGGTGG TTCTGGGCTG CTGTGGAAAG GTTCAACAAT 
GAACAACGTC TAAGACTTTT ACAGTTTGTC ACAGGCACGT CCAGCATTCC CTACGAAGGC 
TTTGCCTCTC TCCGGGGCAG CAACGGGCCC AGGCGCTTCT GCGTGGAGAA GTGGGGGAAG 
ATCACTGCTC TTCCAAGGGC TCACACTTGT TTCAATCGTC TGGACCTTCC CCCTTACCCA 
TCATTCTCTA TGTTATATGA AAAACTGTTG ACAGCAGTAG AAGAGACAAG TACTTTTGGA 
CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW2 ( XM_002193323.3 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_002193359.1 Taeniopygia guttata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

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CloneID	OTd49912
Clone ID Related Accession (Same CDS sequence)	XM_002193323.3
Accession Version	XM_002193323.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	E3 ubiquitin-protein ligase HECW2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044219.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 9, 2019 this sequence version replaced XM_002193323.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CAGCCCGAGA ACACCTGCTC TTCGTGCGCC GTCGCAACCC GCAGCTCCGG 
TACACCCTGA GCCCCGAGAA CCTGCAGAGT TTGGCCTCCC AGGGCACCAT GCCGGAGAAC 
ATGAACTTGC AGCGTGCCAA CAGTGACACT GATTTAGTGA CCTCGGATAG CAGGTCTAGT 
TTGACAGCCA GCATGTATGA ATACACCTTG GGACAGTCTC AGAACCTCAT CATTTTCTGG 
GATATTAAGG AAGAAGTAGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC 
TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA 
ATTGTGTGGC GAATTGAACC TGGCCCCTAC TTTATGGAAT CTGAAATAAA GATCTGTTTT 
AAATACTACC ATGGTGTTAG TGGAGCCCTC AGAGCTACTA CTCCATGTAT CACTGTGAAA 
AACCCTGCTG TGATGATGGG AGTAGAAGGC ACGGATGAAG GTGCTTCTGG AGCTCAGCAC 
TCTAGGAAGT TGGTCAGCTT TACCCTATCA GATATTAGAG CAGCTGGACT TAAAAAAGGC 
ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATTC AGCCAGGCAA GAAAAGTACA 
TTTCCTACGT TTGCTCACCA TGGCCAGGAA AGAAGGTCGA CTATCATCAG TAACACAACT 
AATCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA 
ATTGAGGTTA AAGACAAATT TGCCAAGAGT CGTCCGATCA TCAAGCGCTT CCTTGGGAAG 
TTAACCATAC CTGTTCAGAG ACTTTTGGAG AGACATGCAA TTGGAGACCA GGTTCTCAGC 
TATAATCTTG GCAGAAGGCT CCCAGCTGAT CATGTTAGTG GGTATCTCCA ATTCAAAGTG 
GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGGAC TTTGCTAGGA 
ACAAACTCTG TAAATGGAGA CTTGGGGAGC CCCTCTGATG ATGAGGACAT GCCAGCAGGA 
CATCACGATA CATCCACAGT GTGTTCCAAT GGCCCAGTCT TTGAGGAATC TTCTGGAGAC 
ACAATCCTGA GACAGTCTTT AAGGACTAGT AGGACTCTGG AGACAGACCA GGATGAACTG 
ACCTCTGGTG CTTCAAGATC ATCCCCTCCC AGAGGTCGCC AGGACTCACT AAATGACTAT 
CTCGATGCAA TAGAACACAA TAACCATCCC AGGCCAGGGA CATCTGCCTG TGGTGACCGT 
CCACGAGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCT 
ATGCTTCACA TTGACTCAGA TGAAGAGGAG CACGAGCTGC ATCAAGACCT GGGTTTTCCA 
TCCTCTTTGG AAGATGACGG CGGTTTAGTA ATGCTTAATG GTGCTACCAG GAACGTTGAA 
AGAAATGAAG TAAGTTCCTC ATCAGATCAG GTAATGCCAG GACCTGCAGA GAGTGAAAGG 
GTTTCGGCAT CAGCTGATGA CCAGCAAGAT TCTCTCCCAG AGCTTCCACC TCCAGAGGAA 
GGAGCCGAGT GCCTGCAGGG CTCTCAGGCT GAAGCCCCGG CAGAGCAGGC CGGCAGCGAG 
TCCTCTGCCC CTCCGGAGGC AGAGCAGCCT CCCGGCAGCG CGGACTCGGG AGCTGCTGAA 
GCTGCGGGTG GGAGCAGAAG GGGCGTCAGC GAAACGGAGT CCATCGACCA AGGCTCGGAG 
CCTTCCCAGC TGTCGTCAGA GACGGAGCAG AGCGACCCCG CTCGCACCGA GAGCGTCAGC 
GAGGCCAGCA CCCGGCCCGA GGGGGAGAGC GACGCCGAGG GGGCCGACAG CTCCTGCAAC 
GAGAGCGCGA CCGTGCGGCG CTCCTCCGTG GAGATGCGCT GCTCCTCCTG CGAGAGCGCC 
CGGTTCCCCG AGACCCCAGC CTTCTCTCCC CAGGAGGAGG AGGAGGAGGG AGCCTGTGCC 
CCCGAGGCAG CTGCGGCGGA GCCGGGTGCC GAAGCGCAGG ACTCTGCCGG CGCTGCTGGT 
TCTCTGCCCG CGGTACAGTT ACCTCAGGAG GAGGCGCAGG AGGCTGAGGC AGGTGAGCTG 
CAAGACCAAG AGGAAGCAGA AGAAATCTGG CAAAGAAGAA GAAGCCTGCA GGCAGCAGCT 
GCCCAGAGCC AGTCTCAGGA TGAAGAAGTT GAGGGAGCAC AGGCGGCTTG TGAGGGTGCC 
ACAGCACAGC TTGAAGGAGC TACTGGAGGT TCTCCTGCCA ATGGCCATCA GCCCTTGAGG 
TCCCTGCCTT CAGTGCGTCA GGACGTTAGC CGGTACCAGA GAGTGGATGA GGCTCTGCCA 
CCAAACTGGG AGGCTCGAAT TGACAGCCAC GGACGGATTT TTTACGTGGA CCACGTGAAC 
AGGACAACAA CATGGCAGCG ACCCACAGCT CCCCCAGCCC CACAGATCCT GCAGAGGTCC 
AATTCCATAC AGCAGATGGA GCAGCTGAAT CGCCGGTACC AGAGCATCCG AAGAACAATG 
ACAAATGACA GGCCTGAAGA GCACACAAAT GCCGTGGATG GAGCTGCAGA GGAAGCTGAA 
TTTCACCATT CTAATTCAGA TTTTCGAAGA GAGAATGTGC TGCCTCACTC TACCTCCAGA 
TCCAGACTTA CTTTATTGCT CCAGTCTCCC CCTGTGAAAT TCCTTATCAG CCCAGAGTTC 
TTTACAGTGC TGCATTCTAA CCCTAGTGCC TACCGCATGT TTACAAACAA CACGTGTCTG 
AAGCACATGA TCACCAAAGT CCGGCGGGAC ACCCACCACT TCGAGCGCTA CCAGCACAAC 
CGGGATCTGG TGGGCTTCCT CAACATGTTT GCCAACAAAC AGCTGGAGCT GCCGAGGGGC 
TGGGAAATGA AACACGACCA TCAGGGCAAG GCTTTCTTTG TCGACCACAA CTCGCGCACC 
ACCACGTTCA TCGACCCGCG GCTGCCCCTG CAGAGCAGCA GGCCCACCAG TGCGCTGGTC 
CATCGGCAGC ACCTGACCCG GCAGCGCAGC CACAGTGCTG GGGAGGTCGG AGAGGACTCC 
CGTCATTCAG GACCCCCAGT TTTGCCAAGA CCATCCAGCA CATTTAACAC AGTCAGCAGA 
GCTCAGTACC AGGATGTGGT TCCAGTGGCT TACAATGACA AGATAGTTGC ATTTTTGCGG 
CAGCCCAATA TCTTTGAAAT TCTACAAGAG CGCCAACCAG ATCTTACCAG GAATCATTCA 
CTCAGGGAGA AGATCCAGTT CATCCGGACA GAGGGAACAC CTGGGCTGGT GCGTTTATCC 
AGTGATGCAG ACCTTGTCAT GTTACTCAGC TTGTTTGAGG AGGAAATAAT GTCATATGTG 
CCGCCACATG CCTTACTTCA TCCTAGTTAT TGCCAGTCCC CGCGAGGCTC GCCGGTGTCT 
TCACCTCAGA ACTCTCCAGG TACTCAGCGT GCCAATGCCC GAGCTCCAGC TCCGTACAAA 
AGAGACTTCG AAGCCAAGTT GAGGAACTTC TACAGGAAGT TGGAGACTAA AGGATATGGA 
CAAGGCCCAG GGAAATTGAA ATTAATCATC AGGAGAGATC ACTTATTAGA AGATGCCTTT 
AACCAGATCA TGGGCTACTC GAGAAAAGAC CTGCAGAGAA ATAAACTCTA TGTCACGTTT 
GTTGGGGAAG AAGGGTTGGA CTACAGTGGA CCATCAAGAG AGTTTTTTTT CCTGGTGTCC 
AGGGAGCTCT TCAATCCATA TTATGGTTTG TTTGAATATT CAGCCAACGA TACCTACACA 
GTACAAATAA GTCCCATGTC TGCTTTCGTA GACAATCACC ATGAGTGGTT CAGGTTTAGT 
GGTCGAATTC TTGGTCTTGC TCTAATACAT CAGTATTTGC TGGATGCCTT TTTTACAAGA 
CCCTTTTATA AAGCCCTCCT CCGAATACTC TGTGACCTGA GTGACTTAGA ATATCTGGAT 
GAGGAGTTTC ACCAGAGTTT GCAGTGGATG AAAGACAATG ACATACACGA TATCCTAGAT 
CTCACATTTA CTGTAAATGA AGAAGTTTTT GGGCAGATCA CAGAACGGGA ACTAAAACCA 
GGAGGTGCCA ATATTCCAGT CACTGAAAAG AACAAGAAAG AATATATCGA GAGAATGGTG 
AAATGGAGAA TTGAGAGAGG AGTTGTACAA CAAACAGAAA GTCTGGTTCG TGGTTTCTAT 
GAGGTGGTTG ATGCAAGGCT GGTGTCTGTA TTTGATGCCA GAGAACTGGA ACTGGTTATA 
GCTGGAACAG CAGAAATTGA CTTGAGTGAT TGGAGAAATA ATACAGAGTA CAGAGGAGGT 
TATCATGACA ATCACATTGT AATTCGGTGG TTCTGGGCTG CTGTGGAAAG GTTCAACAAT 
GAACAACGTC TAAGACTTTT ACAGTTTGTC ACAGGCACGT CCAGCATTCC CTACGAAGGC 
TTTGCCTCTC TCCGGGGCAG CAACGGGCCC AGGCGCTTCT GCGTGGAGAA GTGGGGGAAG 
ATCACTGCTC TTCCAAGGGC TCACACTTGT TTCAATCGTC TGGACCTTCC CCCTTACCCA 
TCATTCTCTA TGTTATATGA AAAACTGTTG ACAGCAGTAG AAGAGACAAG TACTTTTGGA 
CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002193359.1
CDS	193..4881
Translation	MASSAREHLLFVRRRNPQLRYTLSPENLQSLASQGTMPENMNLQRANSDTDLVTSDSRSSLTASMYEYTLGQSQNLIIFWDIKEEVDPTDWIGLYHIDENSPANFWDSKNRGVTGTQKGQIVWRIEPGPYFMESEIKICFKYYHGVSGALRATTPCITVKNPAVMMGVEGTDEGASGAQHSRKLVSFTLSDIRAAGLKKGMFFNPDPYLKMSIQPGKKSTFPTFAHHGQERRSTIISNTTNPVWHREKYSFVALLTDVLEIEVKDKFAKSRPIIKRFLGKLTIPVQRLLERHAIGDQVLSYNLGRRLPADHVSGYLQFKVEITSSVHEDASPETVGTLLGTNSVNGDLGSPSDDEDMPAGHHDTSTVCSNGPVFEESSGDTILRQSLRTSRTLETDQDELTSGASRSSPPRGRQDSLNDYLDAIEHNNHPRPGTSACGDRPRGASPKLRSSFPTDTRLNAMLHIDSDEEEHELHQDLGFPSSLEDDGGLVMLNGATRNVERNEVSSSSDQVMPGPAESERVSASADDQQDSLPELPPPEEGAECLQGSQAEAPAEQAGSESSAPPEAEQPPGSADSGAAEAAGGSRRGVSETESIDQGSEPSQLSSETEQSDPARTESVSEASTRPEGESDAEGADSSCNESATVRRSSVEMRCSSCESARFPETPAFSPQEEEEEGACAPEAAAAEPGAEAQDSAGAAGSLPAVQLPQEEAQEAEAGELQDQEEAEEIWQRRRSLQAAAAQSQSQDEEVEGAQAACEGATAQLEGATGGSPANGHQPLRSLPSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQILQRSNSIQQMEQLNRRYQSIRRTMTNDRPEEHTNAVDGAAEEAEFHHSNSDFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSAGEVGEDSRHSGPPVLPRPSSTFNTVSRAQYQDVVPVAYNDKIVAFLRQPNIFEILQERQPDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEIDLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_002193323.3
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002193323.3

ORF Insert Sequence:

ATGGCCAGCT CAGCCCGAGA ACACCTGCTC TTCGTGCGCC GTCGCAACCC GCAGCTCCGG 
TACACCCTGA GCCCCGAGAA CCTGCAGAGT TTGGCCTCCC AGGGCACCAT GCCGGAGAAC 
ATGAACTTGC AGCGTGCCAA CAGTGACACT GATTTAGTGA CCTCGGATAG CAGGTCTAGT 
TTGACAGCCA GCATGTATGA ATACACCTTG GGACAGTCTC AGAACCTCAT CATTTTCTGG 
GATATTAAGG AAGAAGTAGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC 
TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA 
ATTGTGTGGC GAATTGAACC TGGCCCCTAC TTTATGGAAT CTGAAATAAA GATCTGTTTT 
AAATACTACC ATGGTGTTAG TGGAGCCCTC AGAGCTACTA CTCCATGTAT CACTGTGAAA 
AACCCTGCTG TGATGATGGG AGTAGAAGGC ACGGATGAAG GTGCTTCTGG AGCTCAGCAC 
TCTAGGAAGT TGGTCAGCTT TACCCTATCA GATATTAGAG CAGCTGGACT TAAAAAAGGC 
ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATTC AGCCAGGCAA GAAAAGTACA 
TTTCCTACGT TTGCTCACCA TGGCCAGGAA AGAAGGTCGA CTATCATCAG TAACACAACT 
AATCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA 
ATTGAGGTTA AAGACAAATT TGCCAAGAGT CGTCCGATCA TCAAGCGCTT CCTTGGGAAG 
TTAACCATAC CTGTTCAGAG ACTTTTGGAG AGACATGCAA TTGGAGACCA GGTTCTCAGC 
TATAATCTTG GCAGAAGGCT CCCAGCTGAT CATGTTAGTG GGTATCTCCA ATTCAAAGTG 
GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGGAC TTTGCTAGGA 
ACAAACTCTG TAAATGGAGA CTTGGGGAGC CCCTCTGATG ATGAGGACAT GCCAGCAGGA 
CATCACGATA CATCCACAGT GTGTTCCAAT GGCCCAGTCT TTGAGGAATC TTCTGGAGAC 
ACAATCCTGA GACAGTCTTT AAGGACTAGT AGGACTCTGG AGACAGACCA GGATGAACTG 
ACCTCTGGTG CTTCAAGATC ATCCCCTCCC AGAGGTCGCC AGGACTCACT AAATGACTAT 
CTCGATGCAA TAGAACACAA TAACCATCCC AGGCCAGGGA CATCTGCCTG TGGTGACCGT 
CCACGAGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCT 
ATGCTTCACA TTGACTCAGA TGAAGAGGAG CACGAGCTGC ATCAAGACCT GGGTTTTCCA 
TCCTCTTTGG AAGATGACGG CGGTTTAGTA ATGCTTAATG GTGCTACCAG GAACGTTGAA 
AGAAATGAAG TAAGTTCCTC ATCAGATCAG GTAATGCCAG GACCTGCAGA GAGTGAAAGG 
GTTTCGGCAT CAGCTGATGA CCAGCAAGAT TCTCTCCCAG AGCTTCCACC TCCAGAGGAA 
GGAGCCGAGT GCCTGCAGGG CTCTCAGGCT GAAGCCCCGG CAGAGCAGGC CGGCAGCGAG 
TCCTCTGCCC CTCCGGAGGC AGAGCAGCCT CCCGGCAGCG CGGACTCGGG AGCTGCTGAA 
GCTGCGGGTG GGAGCAGAAG GGGCGTCAGC GAAACGGAGT CCATCGACCA AGGCTCGGAG 
CCTTCCCAGC TGTCGTCAGA GACGGAGCAG AGCGACCCCG CTCGCACCGA GAGCGTCAGC 
GAGGCCAGCA CCCGGCCCGA GGGGGAGAGC GACGCCGAGG GGGCCGACAG CTCCTGCAAC 
GAGAGCGCGA CCGTGCGGCG CTCCTCCGTG GAGATGCGCT GCTCCTCCTG CGAGAGCGCC 
CGGTTCCCCG AGACCCCAGC CTTCTCTCCC CAGGAGGAGG AGGAGGAGGG AGCCTGTGCC 
CCCGAGGCAG CTGCGGCGGA GCCGGGTGCC GAAGCGCAGG ACTCTGCCGG CGCTGCTGGT 
TCTCTGCCCG CGGTACAGTT ACCTCAGGAG GAGGCGCAGG AGGCTGAGGC AGGTGAGCTG 
CAAGACCAAG AGGAAGCAGA AGAAATCTGG CAAAGAAGAA GAAGCCTGCA GGCAGCAGCT 
GCCCAGAGCC AGTCTCAGGA TGAAGAAGTT GAGGGAGCAC AGGCGGCTTG TGAGGGTGCC 
ACAGCACAGC TTGAAGGAGC TACTGGAGGT TCTCCTGCCA ATGGCCATCA GCCCTTGAGG 
TCCCTGCCTT CAGTGCGTCA GGACGTTAGC CGGTACCAGA GAGTGGATGA GGCTCTGCCA 
CCAAACTGGG AGGCTCGAAT TGACAGCCAC GGACGGATTT TTTACGTGGA CCACGTGAAC 
AGGACAACAA CATGGCAGCG ACCCACAGCT CCCCCAGCCC CACAGATCCT GCAGAGGTCC 
AATTCCATAC AGCAGATGGA GCAGCTGAAT CGCCGGTACC AGAGCATCCG AAGAACAATG 
ACAAATGACA GGCCTGAAGA GCACACAAAT GCCGTGGATG GAGCTGCAGA GGAAGCTGAA 
TTTCACCATT CTAATTCAGA TTTTCGAAGA GAGAATGTGC TGCCTCACTC TACCTCCAGA 
TCCAGACTTA CTTTATTGCT CCAGTCTCCC CCTGTGAAAT TCCTTATCAG CCCAGAGTTC 
TTTACAGTGC TGCATTCTAA CCCTAGTGCC TACCGCATGT TTACAAACAA CACGTGTCTG 
AAGCACATGA TCACCAAAGT CCGGCGGGAC ACCCACCACT TCGAGCGCTA CCAGCACAAC 
CGGGATCTGG TGGGCTTCCT CAACATGTTT GCCAACAAAC AGCTGGAGCT GCCGAGGGGC 
TGGGAAATGA AACACGACCA TCAGGGCAAG GCTTTCTTTG TCGACCACAA CTCGCGCACC 
ACCACGTTCA TCGACCCGCG GCTGCCCCTG CAGAGCAGCA GGCCCACCAG TGCGCTGGTC 
CATCGGCAGC ACCTGACCCG GCAGCGCAGC CACAGTGCTG GGGAGGTCGG AGAGGACTCC 
CGTCATTCAG GACCCCCAGT TTTGCCAAGA CCATCCAGCA CATTTAACAC AGTCAGCAGA 
GCTCAGTACC AGGATGTGGT TCCAGTGGCT TACAATGACA AGATAGTTGC ATTTTTGCGG 
CAGCCCAATA TCTTTGAAAT TCTACAAGAG CGCCAACCAG ATCTTACCAG GAATCATTCA 
CTCAGGGAGA AGATCCAGTT CATCCGGACA GAGGGAACAC CTGGGCTGGT GCGTTTATCC 
AGTGATGCAG ACCTTGTCAT GTTACTCAGC TTGTTTGAGG AGGAAATAAT GTCATATGTG 
CCGCCACATG CCTTACTTCA TCCTAGTTAT TGCCAGTCCC CGCGAGGCTC GCCGGTGTCT 
TCACCTCAGA ACTCTCCAGG TACTCAGCGT GCCAATGCCC GAGCTCCAGC TCCGTACAAA 
AGAGACTTCG AAGCCAAGTT GAGGAACTTC TACAGGAAGT TGGAGACTAA AGGATATGGA 
CAAGGCCCAG GGAAATTGAA ATTAATCATC AGGAGAGATC ACTTATTAGA AGATGCCTTT 
AACCAGATCA TGGGCTACTC GAGAAAAGAC CTGCAGAGAA ATAAACTCTA TGTCACGTTT 
GTTGGGGAAG AAGGGTTGGA CTACAGTGGA CCATCAAGAG AGTTTTTTTT CCTGGTGTCC 
AGGGAGCTCT TCAATCCATA TTATGGTTTG TTTGAATATT CAGCCAACGA TACCTACACA 
GTACAAATAA GTCCCATGTC TGCTTTCGTA GACAATCACC ATGAGTGGTT CAGGTTTAGT 
GGTCGAATTC TTGGTCTTGC TCTAATACAT CAGTATTTGC TGGATGCCTT TTTTACAAGA 
CCCTTTTATA AAGCCCTCCT CCGAATACTC TGTGACCTGA GTGACTTAGA ATATCTGGAT 
GAGGAGTTTC ACCAGAGTTT GCAGTGGATG AAAGACAATG ACATACACGA TATCCTAGAT 
CTCACATTTA CTGTAAATGA AGAAGTTTTT GGGCAGATCA CAGAACGGGA ACTAAAACCA 
GGAGGTGCCA ATATTCCAGT CACTGAAAAG AACAAGAAAG AATATATCGA GAGAATGGTG 
AAATGGAGAA TTGAGAGAGG AGTTGTACAA CAAACAGAAA GTCTGGTTCG TGGTTTCTAT 
GAGGTGGTTG ATGCAAGGCT GGTGTCTGTA TTTGATGCCA GAGAACTGGA ACTGGTTATA 
GCTGGAACAG CAGAAATTGA CTTGAGTGAT TGGAGAAATA ATACAGAGTA CAGAGGAGGT 
TATCATGACA ATCACATTGT AATTCGGTGG TTCTGGGCTG CTGTGGAAAG GTTCAACAAT 
GAACAACGTC TAAGACTTTT ACAGTTTGTC ACAGGCACGT CCAGCATTCC CTACGAAGGC 
TTTGCCTCTC TCCGGGGCAG CAACGGGCCC AGGCGCTTCT GCGTGGAGAA GTGGGGGAAG 
ATCACTGCTC TTCCAAGGGC TCACACTTGT TTCAATCGTC TGGACCTTCC CCCTTACCCA 
TCATTCTCTA TGTTATATGA AAAACTGTTG ACAGCAGTAG AAGAGACAAG TACTTTTGGA 
CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HECW2 cDNA ORF clone, Taeniopygia guttata(zebra finch)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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