TRPA1 cDNA ORF clone, Taeniopygia guttata(zebra finch)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRPA1
Entrez Gene ID	100221097
Full Name	transient receptor potential cation channel subfamily A member 1
Gene Type	protein-coding
Organism	Taeniopygia guttata(zebra finch)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002197822.3	XP_002197858.2	transient receptor potential cation channel subfamily A member 1
XM_002197822.4	XP_002197858.2	transient receptor potential cation channel subfamily A member 1

Pathways from BioSystems
Gene Ontology

The following TRPA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTd07583	XM_002197822.3 Latest version!	Taeniopygia guttata transient receptor potential cation channel, subfamily A, member 1 (TRPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTd36025	XM_002197822.4 Latest version!	Taeniopygia guttata transient receptor potential cation channel subfamily A member 1 (TRPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRPA1 ( XM_002197822.3 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_002197858.2 Taeniopygia guttata transient receptor potential cation channel, subfamily A, member 1 (TRPA1), mRNA.

Price & Availability↑

CloneID	OTd07583
Clone ID Related Accession (Same CDS sequence)	XM_002197822.3
Accession Version	XM_002197822.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1432483200000
Organism	Taeniopygia guttata(zebra finch)
Product	transient receptor potential cation channel subfamily A member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002198282.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_002197822.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Taeniopygia guttata Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGCT CTCTGCTGCG GCTCTGCCGC CCGCGGGACA AGGCGGCCGC GGCCAGCGCC 
TACCAGGGCG TCGTGTGCGA GGCGGACGCC GCCTCCGTGG CCTCGCAGGA TGTCTTCAAG 
GTTATTTCAG ATGGAAGTGC CTGTAGGCTA CGGAGCTTCA TTAAGAAGAA TCGTTCTGGG 
CTTACAAAAG TGGATGAATT AAATGCCACC CCACTTCATC ACGCTGCAGA AGGAGGGCAA 
ATAGAACTCA TGCAGTTGAT CATAGATGAT TCTTCCTGTG AAGTATTAAA TGTGATGGAT 
TCTTCTGGAA ATACCCCACT GCACTGGGCC ACGAAGAAAA ACCAGGTTGA AAGCGTTCGT 
TTGCTTCTCA GCAGAGGAGC CAATCCAAAC ATCCTCAACT CCAATATGAT GGCACCTTTG 
CATATGGCTG TTCAGTCCCT GCACAATGAA ATTGTGAAGG TTTTAGTCCA ACATAGCAGT 
ACAGATGTTA ACTTGGAAGG TGAAGCAGGG AACACACCTA TAATTGTAGC ATGTTACAAG 
GACAATCCTG AAGCACTGAC ACTTCTGGTT GAAAATGGAG GAAAAATATG TAAACCAAAC 
AAAACAGGAT GTATGCCTAT CCATGCAGCA GCATTTTCAG GCGCAAAAGC ATGCCTGGAA 
ATTCTTTTGA AAAAAGGTGA AGAATTGGGT CACTCTGCTA AAACCCACAT CAATTTTACC 
AATAATGGAA AGTGCAGTCC ACTTCATCTG GCAGTTCAGA GTGGTGACCT CGAAATGATT 
AAAATGTGCA TTGAATTTGG AGCTCAAATT GATCTAAAAC AGAATGAAAA ATGCACAGCA 
CTTCATTTTG CTGCCACTCA AGGAGCAACA GAGATTGTAA AGTTAATGAT GTCATCCTAT 
GCTGGGGAGG AATCCATTAT TGATGCTGTA GATGGGAATA AGGAAACATT GCTTCACAGA 
ACAGCACTGT TTGATCATTA TGAACTGGCG GAGTATTTGA TTTCAATGGG GGCTAATATT 
GATAGTGTTG ACATAGAAGG TCGATCCCCA CTTCTCCTGG CCACTTCCTG TGCATCATGG 
AAAATTGTGA ATTTACTGCT TTCAAAAGGA GCAAATGTGT CTTTAAAAGA TCATCTTGGT 
CGGAATTTCT TGCATTTGAC CGTATTGCAG CCTGGAGGAC TACAGCATCT GAATGAGAAA 
TATCTGCAGA TGGAACATAT TAAAAATCTT GTCGTGGATG AAGATAATGA AGGATGCACT 
CCATTGCATT ATGCATGTAG ACAAGGTGTG GCCCTCTCTG TAAATAATTT ACTCAGCCTC 
AACGTTTCCA TCTACTCTAA GAGCAGGGAC AAGAAGTCGC CGTTACACTT TGCTGCCAGC 
TATGGGCGCA TCAATACTTG CCAACGACTT ATAAGAGATA TGAAAGACAC AAGACTTTTG 
AATGAAGGTG ATAAGAAGGG AATGACTCCC CTTCATTTGG CAGCCCAGAA TGGTCATGAG 
AAGGTAGTTC AGTTTCTTCT GAAGAGAGGG GCTCTCTTCC TCTGTGATTA TAAAGGCTGG 
ACAGCCCTGC ATCATGCTGC CTTTGGAGGA TACACTCGCA CAATGCAAAT AATTTTGGAT 
ACTAATGTGA AGTGTACTGA CAGAGTGGAT GAAGAAGGGA ACACAGCTTT GCACTTGGCT 
GCACGAGAAG GACATGCAAA AGCCGTGAGG TTACTTCTTG ACTATGGCGC GAAAATTCTG 
TTCAACAAAG CAGTAGCTTC TTTTTTCCAT GAAGCAATAC ATAATAGGAG AAAAGACGTG 
GTGTCTGCCG TCATCTTGCA CAAAAGATGG GAAGAAGCTG TTGTGACTTT CTCTCACTCT 
TCCAGTGCCA ATAAGTGTCC TTTGCTGGAA ATGGTTGAGT ATCTTCCTGA TTCATTTAAA 
CTGGTGCTGG ACAACTGCAT AATTGAGTCT TCAGAGGAAA AGACAAGTCG AGACTTCTAT 
ATTGAATATA ACTTCAGATA TCTTCAGTGT CCCCTGACAC TTAACAAGAA AGTAAAGGAT 
GGTGAAGATA TTTTCTATGA ACCACTTACG ACTTTAAATG CCATGGTGCG CCACAATCGC 
ATGGAACTAC TCAGTCATCC TGTGTGTAAA GAATATTTGC TTATGAAATG GATGGCCTAT 
GGGTTTCGAG CACATTTAAT GAATTTAGGC ATATATTCTC TTGGTCTCAT TCCCCTGACT 
CTTCTGGTCA CTCACATACA ACCAGGAAGA CCTTTGAATG GAACAGAGCT CTATGAAGCA 
AGACCATTAG AATACGAGGA CTCATACTTC ACAAGGGTGT GTATGTGTTT AGTTTTAATT 
ATGAGTTTAC TGGGCATCTG CAAAGAAATA TTTCAGCTGA TTCAGCAGAA ATTGAAATAT 
TTGTTGGATT ACTCCAATCT ACTGGACTGG ACCATTTATA CTACAAGCAT AATTTTTGTG 
TCTTCTTTAT TTATTAATAC CCCAGCTCAT TTGCAGTGGG AATGTGGAGC AATTGCTGTA 
TACTTGTCCT GGATGAACTT CTTGCTTTAT CTTCAACGAT TTGAGAACTA TGGAATCTAT 
GTTGTGATGT TCTGGGAAAT TTTGAGGACT TTGATTCGGA TTGCTGTGGT TTTCTTTTTC 
CTGATACTGG CCTTTGGCCT GAGTTTCTTT GTCCTTTTGG GTTCACAGCA AACATACAGC 
ACACCTCTGC TTTCTGTAAT GAAGACATTT GCAATGATGC TGGGAGACAT TAATTACCAT 
GATGCATTCC TTGATCCTTT ACTAAGCAGT GAATTGCCAT ATCCTTTCCT GAGTTACACA 
GTTCTCATTA TATTTACCTT GCTTATTCCA ATCCTTCTTA TGAACTTGCT AATTGGTTTG 
GCTGTTGGTG ATATCGCTGA AGTGCAAAAA TATGCTGCAC TCAAAAGGAT TGCAATGCAG 
GTTAATCTTC ATACCAACTT AGAGAAGAAG CTGCCATTTT GGTTCTTAAG TCGGGTGGAC 
CAGGAATCAA TCACTGTATA TCCCAACAGG CCAAGATACT GTGGATTTAT GAGCGTGTTC 
CAATATTGCT TTGGGTGTGA GGACTCTACC ACATATGCAC AGAGCCCTGA TACAACACTA 
GAACTGGAAG TTCTGAAGCA GAAATACAGG CTCAAAGATA TATCAACATT GTTGGAAAAG 
CAACATGACC TCATTAAACT GATTATCCAA AAAATGGAGA TAGTGTCAGA GGCTGAGGAT 
GAAGACAGCA ATGATTTGTT TCAGCACAAA TTTAGGAAAA GGCAGTTAGA GCACAAGAAT 
AGTAAATGGG ATACAGTGTT AAAAGCTGTT AAAACAAATG TGCCTAACCC AAGCACAGAA 
AAGAACAATA GCTTCTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002197858.2
CDS	1..3381
Translation	MKRSLLRLCRPRDKAAAASAYQGVVCEADAASVASQDVFKVISDGSACRLRSFIKKNRSGLTKVDELNATPLHHAAEGGQIELMQLIIDDSSCEVLNVMDSSGNTPLHWATKKNQVESVRLLLSRGANPNILNSNMMAPLHMAVQSLHNEIVKVLVQHSSTDVNLEGEAGNTPIIVACYKDNPEALTLLVENGGKICKPNKTGCMPIHAAAFSGAKACLEILLKKGEELGHSAKTHINFTNNGKCSPLHLAVQSGDLEMIKMCIEFGAQIDLKQNEKCTALHFAATQGATEIVKLMMSSYAGEESIIDAVDGNKETLLHRTALFDHYELAEYLISMGANIDSVDIEGRSPLLLATSCASWKIVNLLLSKGANVSLKDHLGRNFLHLTVLQPGGLQHLNEKYLQMEHIKNLVVDEDNEGCTPLHYACRQGVALSVNNLLSLNVSIYSKSRDKKSPLHFAASYGRINTCQRLIRDMKDTRLLNEGDKKGMTPLHLAAQNGHEKVVQFLLKRGALFLCDYKGWTALHHAAFGGYTRTMQIILDTNVKCTDRVDEEGNTALHLAAREGHAKAVRLLLDYGAKILFNKAVASFFHEAIHNRRKDVVSAVILHKRWEEAVVTFSHSSSANKCPLLEMVEYLPDSFKLVLDNCIIESSEEKTSRDFYIEYNFRYLQCPLTLNKKVKDGEDIFYEPLTTLNAMVRHNRMELLSHPVCKEYLLMKWMAYGFRAHLMNLGIYSLGLIPLTLLVTHIQPGRPLNGTELYEARPLEYEDSYFTRVCMCLVLIMSLLGICKEIFQLIQQKLKYLLDYSNLLDWTIYTTSIIFVSSLFINTPAHLQWECGAIAVYLSWMNFLLYLQRFENYGIYVVMFWEILRTLIRIAVVFFFLILAFGLSFFVLLGSQQTYSTPLLSVMKTFAMMLGDINYHDAFLDPLLSSELPYPFLSYTVLIIFTLLIPILLMNLLIGLAVGDIAEVQKYAALKRIAMQVNLHTNLEKKLPFWFLSRVDQESITVYPNRPRYCGFMSVFQYCFGCEDSTTYAQSPDTTLELEVLKQKYRLKDISTLLEKQHDLIKLIIQKMEIVSEAEDEDSNDLFQHKFRKRQLEHKNSKWDTVLKAVKTNVPNPSTEKNNSFF

Target ORF information:

RefSeq Version	XM_002197822.3
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata transient receptor potential cation channel, subfamily A, member 1 (TRPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002197822.3

ORF Insert Sequence:

ATGAAGCGCT CTCTGCTGCG GCTCTGCCGC CCGCGGGACA AGGCGGCCGC GGCCAGCGCC 
TACCAGGGCG TCGTGTGCGA GGCGGACGCC GCCTCCGTGG CCTCGCAGGA TGTCTTCAAG 
GTTATTTCAG ATGGAAGTGC CTGTAGGCTA CGGAGCTTCA TTAAGAAGAA TCGTTCTGGG 
CTTACAAAAG TGGATGAATT AAATGCCACC CCACTTCATC ACGCTGCAGA AGGAGGGCAA 
ATAGAACTCA TGCAGTTGAT CATAGATGAT TCTTCCTGTG AAGTATTAAA TGTGATGGAT 
TCTTCTGGAA ATACCCCACT GCACTGGGCC ACGAAGAAAA ACCAGGTTGA AAGCGTTCGT 
TTGCTTCTCA GCAGAGGAGC CAATCCAAAC ATCCTCAACT CCAATATGAT GGCACCTTTG 
CATATGGCTG TTCAGTCCCT GCACAATGAA ATTGTGAAGG TTTTAGTCCA ACATAGCAGT 
ACAGATGTTA ACTTGGAAGG TGAAGCAGGG AACACACCTA TAATTGTAGC ATGTTACAAG 
GACAATCCTG AAGCACTGAC ACTTCTGGTT GAAAATGGAG GAAAAATATG TAAACCAAAC 
AAAACAGGAT GTATGCCTAT CCATGCAGCA GCATTTTCAG GCGCAAAAGC ATGCCTGGAA 
ATTCTTTTGA AAAAAGGTGA AGAATTGGGT CACTCTGCTA AAACCCACAT CAATTTTACC 
AATAATGGAA AGTGCAGTCC ACTTCATCTG GCAGTTCAGA GTGGTGACCT CGAAATGATT 
AAAATGTGCA TTGAATTTGG AGCTCAAATT GATCTAAAAC AGAATGAAAA ATGCACAGCA 
CTTCATTTTG CTGCCACTCA AGGAGCAACA GAGATTGTAA AGTTAATGAT GTCATCCTAT 
GCTGGGGAGG AATCCATTAT TGATGCTGTA GATGGGAATA AGGAAACATT GCTTCACAGA 
ACAGCACTGT TTGATCATTA TGAACTGGCG GAGTATTTGA TTTCAATGGG GGCTAATATT 
GATAGTGTTG ACATAGAAGG TCGATCCCCA CTTCTCCTGG CCACTTCCTG TGCATCATGG 
AAAATTGTGA ATTTACTGCT TTCAAAAGGA GCAAATGTGT CTTTAAAAGA TCATCTTGGT 
CGGAATTTCT TGCATTTGAC CGTATTGCAG CCTGGAGGAC TACAGCATCT GAATGAGAAA 
TATCTGCAGA TGGAACATAT TAAAAATCTT GTCGTGGATG AAGATAATGA AGGATGCACT 
CCATTGCATT ATGCATGTAG ACAAGGTGTG GCCCTCTCTG TAAATAATTT ACTCAGCCTC 
AACGTTTCCA TCTACTCTAA GAGCAGGGAC AAGAAGTCGC CGTTACACTT TGCTGCCAGC 
TATGGGCGCA TCAATACTTG CCAACGACTT ATAAGAGATA TGAAAGACAC AAGACTTTTG 
AATGAAGGTG ATAAGAAGGG AATGACTCCC CTTCATTTGG CAGCCCAGAA TGGTCATGAG 
AAGGTAGTTC AGTTTCTTCT GAAGAGAGGG GCTCTCTTCC TCTGTGATTA TAAAGGCTGG 
ACAGCCCTGC ATCATGCTGC CTTTGGAGGA TACACTCGCA CAATGCAAAT AATTTTGGAT 
ACTAATGTGA AGTGTACTGA CAGAGTGGAT GAAGAAGGGA ACACAGCTTT GCACTTGGCT 
GCACGAGAAG GACATGCAAA AGCCGTGAGG TTACTTCTTG ACTATGGCGC GAAAATTCTG 
TTCAACAAAG CAGTAGCTTC TTTTTTCCAT GAAGCAATAC ATAATAGGAG AAAAGACGTG 
GTGTCTGCCG TCATCTTGCA CAAAAGATGG GAAGAAGCTG TTGTGACTTT CTCTCACTCT 
TCCAGTGCCA ATAAGTGTCC TTTGCTGGAA ATGGTTGAGT ATCTTCCTGA TTCATTTAAA 
CTGGTGCTGG ACAACTGCAT AATTGAGTCT TCAGAGGAAA AGACAAGTCG AGACTTCTAT 
ATTGAATATA ACTTCAGATA TCTTCAGTGT CCCCTGACAC TTAACAAGAA AGTAAAGGAT 
GGTGAAGATA TTTTCTATGA ACCACTTACG ACTTTAAATG CCATGGTGCG CCACAATCGC 
ATGGAACTAC TCAGTCATCC TGTGTGTAAA GAATATTTGC TTATGAAATG GATGGCCTAT 
GGGTTTCGAG CACATTTAAT GAATTTAGGC ATATATTCTC TTGGTCTCAT TCCCCTGACT 
CTTCTGGTCA CTCACATACA ACCAGGAAGA CCTTTGAATG GAACAGAGCT CTATGAAGCA 
AGACCATTAG AATACGAGGA CTCATACTTC ACAAGGGTGT GTATGTGTTT AGTTTTAATT 
ATGAGTTTAC TGGGCATCTG CAAAGAAATA TTTCAGCTGA TTCAGCAGAA ATTGAAATAT 
TTGTTGGATT ACTCCAATCT ACTGGACTGG ACCATTTATA CTACAAGCAT AATTTTTGTG 
TCTTCTTTAT TTATTAATAC CCCAGCTCAT TTGCAGTGGG AATGTGGAGC AATTGCTGTA 
TACTTGTCCT GGATGAACTT CTTGCTTTAT CTTCAACGAT TTGAGAACTA TGGAATCTAT 
GTTGTGATGT TCTGGGAAAT TTTGAGGACT TTGATTCGGA TTGCTGTGGT TTTCTTTTTC 
CTGATACTGG CCTTTGGCCT GAGTTTCTTT GTCCTTTTGG GTTCACAGCA AACATACAGC 
ACACCTCTGC TTTCTGTAAT GAAGACATTT GCAATGATGC TGGGAGACAT TAATTACCAT 
GATGCATTCC TTGATCCTTT ACTAAGCAGT GAATTGCCAT ATCCTTTCCT GAGTTACACA 
GTTCTCATTA TATTTACCTT GCTTATTCCA ATCCTTCTTA TGAACTTGCT AATTGGTTTG 
GCTGTTGGTG ATATCGCTGA AGTGCAAAAA TATGCTGCAC TCAAAAGGAT TGCAATGCAG 
GTTAATCTTC ATACCAACTT AGAGAAGAAG CTGCCATTTT GGTTCTTAAG TCGGGTGGAC 
CAGGAATCAA TCACTGTATA TCCCAACAGG CCAAGATACT GTGGATTTAT GAGCGTGTTC 
CAATATTGCT TTGGGTGTGA GGACTCTACC ACATATGCAC AGAGCCCTGA TACAACACTA 
GAACTGGAAG TTCTGAAGCA GAAATACAGG CTCAAAGATA TATCAACATT GTTGGAAAAG 
CAACATGACC TCATTAAACT GATTATCCAA AAAATGGAGA TAGTGTCAGA GGCTGAGGAT 
GAAGACAGCA ATGATTTGTT TCAGCACAAA TTTAGGAAAA GGCAGTTAGA GCACAAGAAT 
AGTAAATGGG ATACAGTGTT AAAAGCTGTT AAAACAAATG TGCCTAACCC AAGCACAGAA 
AAGAACAATA GCTTCTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRPA1 ( XM_002197822.4 ) cDNA ORF clone, Taeniopygia guttata(zebra finch) -> XP_002197858.2 Taeniopygia guttata transient receptor potential cation channel subfamily A member 1 (TRPA1), mRNA.

Price & Availability↑

CloneID	OTd36025
Clone ID Related Accession (Same CDS sequence)	XM_002197822.4
Accession Version	XM_002197822.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565193600000
Organism	Taeniopygia guttata(zebra finch)
Product	transient receptor potential cation channel subfamily A member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044213.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 9, 2019 this sequence version replaced XM_002197822.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Taeniopygia guttata Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCGCT CTCTGCTGCG GCTCTGCCGC CCGCGGGACA AGGCGGCCGC GGCCAGCGCC 
TACCAGGGCG TCGTGTGCGA GGCGGACGCC GCCTCCGTGG CCTCGCAGGA TGTCTTCAAG 
GTTATTTCAG ATGGAAGTGC CTGTAGGCTA CGGAGCTTCA TTAAGAAGAA TCGTTCTGGG 
CTTACAAAAG TGGATGAATT AAATGCCACC CCACTTCATC ACGCTGCAGA AGGAGGGCAA 
ATAGAACTCA TGCAGTTGAT CATAGATGAT TCTTCCTGTG AAGTATTAAA TGTGATGGAT 
TCTTCTGGAA ATACCCCACT GCACTGGGCC ACGAAGAAAA ACCAGGTTGA AAGCGTTCGT 
TTGCTTCTCA GCAGAGGAGC CAATCCAAAC ATCCTCAACT CCAATATGAT GGCACCTTTG 
CATATGGCTG TTCAGTCCCT GCACAATGAA ATTGTGAAGG TTTTAGTCCA ACATAGCAGT 
ACAGATGTTA ACTTGGAAGG TGAAGCAGGG AACACACCTA TAATTGTAGC ATGTTACAAG 
GACAATCCTG AAGCACTGAC ACTTCTGGTT GAAAATGGAG GAAAAATATG TAAACCAAAC 
AAAACAGGAT GTATGCCTAT CCATGCAGCA GCATTTTCAG GTGCAAAAGC ATGCCTGGAA 
ATTCTTTTGA AAAAAGGTGA AGAATTGGGT CACTCTGCTA AAACCCACAT CAATTTTACC 
AATAATGGAA AGTGCAGTCC ACTTCATCTG GCAGTTCAGA GTGGCGACCT TGAAATGATT 
AAAATGTGCA TTGAATTCGG AGCTCAAATT GATCTAAAAC AGAATGAAAA ATGCACGGCA 
CTTCATTTTG CTGCCACTCA AGGAGCGACA GAGATTGTAA AGCTAATGAT GTCATCCTAT 
GCTGGGGAGG AATCCATTAT TGATGCTGTA GATGGGAATA AGGAAACATT GCTTCACAGA 
ACAGCACTGT TTGATCATTA TGAACTGGCG GAGTATTTGA TTTCAATGGG GGCTAATATT 
GATAGTGTTG ACATAGAAGG TCGATCCCCA CTTCTCCTGG CCACTTCCTG TGCATCATGG 
AAAATTGTGA ATTTACTGCT TTCAAAAGGA GCAAATGTGT CTTTAAAAGA TCATCTTGGT 
CGGAATTTCT TGCATTTGAC CGTATTGCAG CCTGGAGGAC TACAGCATCT GAATGAGAAA 
TATCTGCAGA TGGAACATAT TAAAAATCTT GTCGTGGATG AAGATAATGA AGGATGCACT 
CCATTGCATT ATGCATGTAG ACAAGGTGTG GCCCTTTCTG TAAATAATTT ACTCAGCCTC 
AACGTTTCCA TCTACTCTAA GAGCAGGGAC AAGAAGTCGC CGTTACACTT TGCTGCCAGC 
TATGGGCGTA TCAATACTTG CCAACGACTT ATAAGAGATA TGAAAGACAC AAGACTTTTG 
AATGAAGGTG ATAAGAAGGG AATGACTCCC CTTCATTTGG CAGCCCAGAA TGGTCATGAG 
AAAGTAGTTC AGTTTCTTCT GAAGAGAGGG GCTCTTTTCC TCTGTGATTA TAAAGGCTGG 
ACAGCCCTGC ATCATGCTGC CTTTGGAGGA TACACTCGCA CAATGCAGAT AATTTTGGAT 
ACTAATGTGA AGTGTACTGA CAGAGTGGAT GAAGAAGGGA ACACAGCTTT GCACTTGGCT 
GCACGAGAAG GACATGCAAA AGCAGTGAGG TTACTTCTTG ACTATGGCGC GAAAATTCTG 
TTCAACAAAG CAGTAGCTTC TTTTTTCCAT GAAGCAATAC ATAATAGGAG AAAAGACGTG 
GTGTCTGCCG TCATCTTGCA CAAAAGATGG GAAGAAGCTG TTGTGACTTT CTCTCACTCT 
TCCAGTGCCA ATAAGTGTCC TTTGCTGGAA ATGGTTGAGT ATCTTCCTGA TTCATTTAAA 
CTGGTGTTGG ACAACTGCAT AATTGAGTCT TCAGAGGAAA AGACAAGTCG AGACTTCTAT 
ATTGAATATA ACTTCAGATA TCTTCAGTGT CCCCTGACAC TTAACAAGAA AGTAAAGGAT 
GGTGAAGATA TTTTCTATGA ACCACTTACG ACTTTAAATG CCATGGTGCG CCACAATCGC 
ATGGAACTAC TCAGTCATCC TGTGTGTAAA GAATATTTGC TTATGAAATG GATGGCCTAT 
GGGTTTCGAG CACATTTAAT GAATTTAGGC ATATATTCTC TTGGTCTCAT TCCCCTGACT 
CTTCTGGTCA CTCACATACA ACCAGGAAGA CCTTTGAATG GAACAGAGCT CTATGAAGCA 
AGACCATTAG AATATGAGGA CTCATACTTC ACAAGGGTGT GTATGTGTTT AGTTTTAATT 
ATGAGTTTAC TGGGCATCTG CAAAGAAATA TTTCAGCTGA TTCAGCAGAA ATTGAAATAT 
TTGTTGGATT ACTCCAATCT ACTGGACTGG ACCATTTATA CTACAAGCAT AATTTTTGTG 
TCTTCTTTAT TTATTAATAC CCCAGCTCAT TTGCAGTGGG AATGTGGAGC AATTGCTGTA 
TACTTGTCCT GGATGAACTT CTTGCTTTAT CTTCAACGAT TTGAGAACTA TGGAATCTAT 
GTTGTGATGT TCTGGGAAAT TTTGAGGACT TTGATTCGGA TTGCTGTGGT TTTCTTTTTC 
CTGATACTGG CCTTTGGCCT GAGTTTCTTT GTCCTTTTGG GTTCACAGCA AACATACAGC 
ACACCTCTGC TTTCTGTAAT GAAGACATTT GCAATGATGC TGGGAGACAT TAATTACCAT 
GATGCATTCC TTGATCCTTT ACTAAGCAGT GAATTGCCAT ATCCTTTCCT GAGTTACACA 
GTTCTCATTA TATTTACCTT GCTTATTCCA ATCCTTCTTA TGAACTTGCT AATTGGTTTG 
GCTGTTGGTG ATATCGCTGA AGTGCAAAAA TATGCTGCAC TCAAAAGGAT TGCAATGCAG 
GTTAATCTTC ATACCAACTT AGAGAAGAAG CTGCCATTTT GGTTCTTAAG TCGGGTGGAC 
CAGGAATCAA TCACTGTATA TCCCAACAGG CCAAGATACT GTGGATTTAT GAGCGTGTTC 
CAATATTGCT TTGGGTGTGA GGACTCTACC ACATATGCAC AGAGCCCTGA TACAACACTA 
GAACTGGAAG TTCTGAAGCA GAAATACAGG CTCAAAGATA TATCAACATT GTTGGAAAAG 
CAACATGACC TCATTAAACT GATTATCCAA AAAATGGAGA TAGTGTCAGA GGCTGAGGAT 
GAAGACAGCA ATGATTTGTT TCAGCACAAA TTTAGGAAAA GGCAGTTAGA GCACAAGAAT 
AGTAAATGGG ATACAGTGTT AAAAGCTGTT AAAACAAATG TGCCTAACCC AAGCACAGAA 
AAGAACAATA GCTTCTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002197858.2
CDS	36..3416
Translation	MKRSLLRLCRPRDKAAAASAYQGVVCEADAASVASQDVFKVISDGSACRLRSFIKKNRSGLTKVDELNATPLHHAAEGGQIELMQLIIDDSSCEVLNVMDSSGNTPLHWATKKNQVESVRLLLSRGANPNILNSNMMAPLHMAVQSLHNEIVKVLVQHSSTDVNLEGEAGNTPIIVACYKDNPEALTLLVENGGKICKPNKTGCMPIHAAAFSGAKACLEILLKKGEELGHSAKTHINFTNNGKCSPLHLAVQSGDLEMIKMCIEFGAQIDLKQNEKCTALHFAATQGATEIVKLMMSSYAGEESIIDAVDGNKETLLHRTALFDHYELAEYLISMGANIDSVDIEGRSPLLLATSCASWKIVNLLLSKGANVSLKDHLGRNFLHLTVLQPGGLQHLNEKYLQMEHIKNLVVDEDNEGCTPLHYACRQGVALSVNNLLSLNVSIYSKSRDKKSPLHFAASYGRINTCQRLIRDMKDTRLLNEGDKKGMTPLHLAAQNGHEKVVQFLLKRGALFLCDYKGWTALHHAAFGGYTRTMQIILDTNVKCTDRVDEEGNTALHLAAREGHAKAVRLLLDYGAKILFNKAVASFFHEAIHNRRKDVVSAVILHKRWEEAVVTFSHSSSANKCPLLEMVEYLPDSFKLVLDNCIIESSEEKTSRDFYIEYNFRYLQCPLTLNKKVKDGEDIFYEPLTTLNAMVRHNRMELLSHPVCKEYLLMKWMAYGFRAHLMNLGIYSLGLIPLTLLVTHIQPGRPLNGTELYEARPLEYEDSYFTRVCMCLVLIMSLLGICKEIFQLIQQKLKYLLDYSNLLDWTIYTTSIIFVSSLFINTPAHLQWECGAIAVYLSWMNFLLYLQRFENYGIYVVMFWEILRTLIRIAVVFFFLILAFGLSFFVLLGSQQTYSTPLLSVMKTFAMMLGDINYHDAFLDPLLSSELPYPFLSYTVLIIFTLLIPILLMNLLIGLAVGDIAEVQKYAALKRIAMQVNLHTNLEKKLPFWFLSRVDQESITVYPNRPRYCGFMSVFQYCFGCEDSTTYAQSPDTTLELEVLKQKYRLKDISTLLEKQHDLIKLIIQKMEIVSEAEDEDSNDLFQHKFRKRQLEHKNSKWDTVLKAVKTNVPNPSTEKNNSFF

Target ORF information:

RefSeq Version	XM_002197822.4
Organism	Taeniopygia guttata(zebra finch)
Definition	Taeniopygia guttata transient receptor potential cation channel subfamily A member 1 (TRPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002197822.4

ORF Insert Sequence:

ATGAAGCGCT CTCTGCTGCG GCTCTGCCGC CCGCGGGACA AGGCGGCCGC GGCCAGCGCC 
TACCAGGGCG TCGTGTGCGA GGCGGACGCC GCCTCCGTGG CCTCGCAGGA TGTCTTCAAG 
GTTATTTCAG ATGGAAGTGC CTGTAGGCTA CGGAGCTTCA TTAAGAAGAA TCGTTCTGGG 
CTTACAAAAG TGGATGAATT AAATGCCACC CCACTTCATC ACGCTGCAGA AGGAGGGCAA 
ATAGAACTCA TGCAGTTGAT CATAGATGAT TCTTCCTGTG AAGTATTAAA TGTGATGGAT 
TCTTCTGGAA ATACCCCACT GCACTGGGCC ACGAAGAAAA ACCAGGTTGA AAGCGTTCGT 
TTGCTTCTCA GCAGAGGAGC CAATCCAAAC ATCCTCAACT CCAATATGAT GGCACCTTTG 
CATATGGCTG TTCAGTCCCT GCACAATGAA ATTGTGAAGG TTTTAGTCCA ACATAGCAGT 
ACAGATGTTA ACTTGGAAGG TGAAGCAGGG AACACACCTA TAATTGTAGC ATGTTACAAG 
GACAATCCTG AAGCACTGAC ACTTCTGGTT GAAAATGGAG GAAAAATATG TAAACCAAAC 
AAAACAGGAT GTATGCCTAT CCATGCAGCA GCATTTTCAG GTGCAAAAGC ATGCCTGGAA 
ATTCTTTTGA AAAAAGGTGA AGAATTGGGT CACTCTGCTA AAACCCACAT CAATTTTACC 
AATAATGGAA AGTGCAGTCC ACTTCATCTG GCAGTTCAGA GTGGCGACCT TGAAATGATT 
AAAATGTGCA TTGAATTCGG AGCTCAAATT GATCTAAAAC AGAATGAAAA ATGCACGGCA 
CTTCATTTTG CTGCCACTCA AGGAGCGACA GAGATTGTAA AGCTAATGAT GTCATCCTAT 
GCTGGGGAGG AATCCATTAT TGATGCTGTA GATGGGAATA AGGAAACATT GCTTCACAGA 
ACAGCACTGT TTGATCATTA TGAACTGGCG GAGTATTTGA TTTCAATGGG GGCTAATATT 
GATAGTGTTG ACATAGAAGG TCGATCCCCA CTTCTCCTGG CCACTTCCTG TGCATCATGG 
AAAATTGTGA ATTTACTGCT TTCAAAAGGA GCAAATGTGT CTTTAAAAGA TCATCTTGGT 
CGGAATTTCT TGCATTTGAC CGTATTGCAG CCTGGAGGAC TACAGCATCT GAATGAGAAA 
TATCTGCAGA TGGAACATAT TAAAAATCTT GTCGTGGATG AAGATAATGA AGGATGCACT 
CCATTGCATT ATGCATGTAG ACAAGGTGTG GCCCTTTCTG TAAATAATTT ACTCAGCCTC 
AACGTTTCCA TCTACTCTAA GAGCAGGGAC AAGAAGTCGC CGTTACACTT TGCTGCCAGC 
TATGGGCGTA TCAATACTTG CCAACGACTT ATAAGAGATA TGAAAGACAC AAGACTTTTG 
AATGAAGGTG ATAAGAAGGG AATGACTCCC CTTCATTTGG CAGCCCAGAA TGGTCATGAG 
AAAGTAGTTC AGTTTCTTCT GAAGAGAGGG GCTCTTTTCC TCTGTGATTA TAAAGGCTGG 
ACAGCCCTGC ATCATGCTGC CTTTGGAGGA TACACTCGCA CAATGCAGAT AATTTTGGAT 
ACTAATGTGA AGTGTACTGA CAGAGTGGAT GAAGAAGGGA ACACAGCTTT GCACTTGGCT 
GCACGAGAAG GACATGCAAA AGCAGTGAGG TTACTTCTTG ACTATGGCGC GAAAATTCTG 
TTCAACAAAG CAGTAGCTTC TTTTTTCCAT GAAGCAATAC ATAATAGGAG AAAAGACGTG 
GTGTCTGCCG TCATCTTGCA CAAAAGATGG GAAGAAGCTG TTGTGACTTT CTCTCACTCT 
TCCAGTGCCA ATAAGTGTCC TTTGCTGGAA ATGGTTGAGT ATCTTCCTGA TTCATTTAAA 
CTGGTGTTGG ACAACTGCAT AATTGAGTCT TCAGAGGAAA AGACAAGTCG AGACTTCTAT 
ATTGAATATA ACTTCAGATA TCTTCAGTGT CCCCTGACAC TTAACAAGAA AGTAAAGGAT 
GGTGAAGATA TTTTCTATGA ACCACTTACG ACTTTAAATG CCATGGTGCG CCACAATCGC 
ATGGAACTAC TCAGTCATCC TGTGTGTAAA GAATATTTGC TTATGAAATG GATGGCCTAT 
GGGTTTCGAG CACATTTAAT GAATTTAGGC ATATATTCTC TTGGTCTCAT TCCCCTGACT 
CTTCTGGTCA CTCACATACA ACCAGGAAGA CCTTTGAATG GAACAGAGCT CTATGAAGCA 
AGACCATTAG AATATGAGGA CTCATACTTC ACAAGGGTGT GTATGTGTTT AGTTTTAATT 
ATGAGTTTAC TGGGCATCTG CAAAGAAATA TTTCAGCTGA TTCAGCAGAA ATTGAAATAT 
TTGTTGGATT ACTCCAATCT ACTGGACTGG ACCATTTATA CTACAAGCAT AATTTTTGTG 
TCTTCTTTAT TTATTAATAC CCCAGCTCAT TTGCAGTGGG AATGTGGAGC AATTGCTGTA 
TACTTGTCCT GGATGAACTT CTTGCTTTAT CTTCAACGAT TTGAGAACTA TGGAATCTAT 
GTTGTGATGT TCTGGGAAAT TTTGAGGACT TTGATTCGGA TTGCTGTGGT TTTCTTTTTC 
CTGATACTGG CCTTTGGCCT GAGTTTCTTT GTCCTTTTGG GTTCACAGCA AACATACAGC 
ACACCTCTGC TTTCTGTAAT GAAGACATTT GCAATGATGC TGGGAGACAT TAATTACCAT 
GATGCATTCC TTGATCCTTT ACTAAGCAGT GAATTGCCAT ATCCTTTCCT GAGTTACACA 
GTTCTCATTA TATTTACCTT GCTTATTCCA ATCCTTCTTA TGAACTTGCT AATTGGTTTG 
GCTGTTGGTG ATATCGCTGA AGTGCAAAAA TATGCTGCAC TCAAAAGGAT TGCAATGCAG 
GTTAATCTTC ATACCAACTT AGAGAAGAAG CTGCCATTTT GGTTCTTAAG TCGGGTGGAC 
CAGGAATCAA TCACTGTATA TCCCAACAGG CCAAGATACT GTGGATTTAT GAGCGTGTTC 
CAATATTGCT TTGGGTGTGA GGACTCTACC ACATATGCAC AGAGCCCTGA TACAACACTA 
GAACTGGAAG TTCTGAAGCA GAAATACAGG CTCAAAGATA TATCAACATT GTTGGAAAAG 
CAACATGACC TCATTAAACT GATTATCCAA AAAATGGAGA TAGTGTCAGA GGCTGAGGAT 
GAAGACAGCA ATGATTTGTT TCAGCACAAA TTTAGGAAAA GGCAGTTAGA GCACAAGAAT 
AGTAAATGGG ATACAGTGTT AAAAGCTGTT AAAACAAATG TGCCTAACCC AAGCACAGAA 
AAGAACAATA GCTTCTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TRPA1 cDNA ORF clone, Taeniopygia guttata(zebra finch)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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