THBS1 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	THBS1
Entrez Gene ID	492313
Full Name	thrombospondin 1
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001244536.1	NP_001231465.1	thrombospondin-1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

MiR-222 inhibits apoptosis in porcine follicular granulosa cells by targeting the THBS1 gene.
Zhu W, Yang M, Shang J, Xu Y, Wang Y, Tao Q, Zhang L, Ding Y, Chen Y, Zhao D, Wang C, Chu M, Yin Z, Zhang X
Animal science journal = Nihon chikusan Gakkaiho90(6)719-727(2019 Jun)

Glucose regulation of thrombospondin and its role in the modulation of smooth muscle cell proliferation.
Maile LA, Allen LB, Hanzaker CF, Gollahon KA, Dunbar P, Clemmons DR
Experimental diabetes research2010(2010)

Thrombospondin-1 mRNA expression in experimental kidney transplantation with heart-beating and non-heart-beating donors.
Lario S, Bescós M, Campos B, Mur C, Luque P, Alvarez R, Campistol JM
Journal of nephrology20(5)588-95(2007 Sep-Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following THBS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe28327	NM_001244536.1 Latest version!	Sus scrofa thrombospondin 1 (THBS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THBS1 ( NM_001244536.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001231465.1 Sus scrofa thrombospondin 1 (THBS1), mRNA.

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CloneID	OSe28327
Clone ID Related Accession (Same CDS sequence)	NM_001244536.1
Accession Version	NM_001244536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1574006400000
Organism	Sus scrofa(pig)
Product	thrombospondin-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH006601.1, CN157497.1, AY773342.1, DN121867.1, AJ666399.1, EH009145.1, EH007302.1, FD631606.1, EH008936.1, CB475299.1 and BQ601960.1. On Sep 18, 2011 this sequence version replaced XM_001927442.1. Sequence Note: This RefSeq record was created from transcripts of different strains because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1487420, SAMEA1487451 [ECO:0000350] ##Evidence-Data-END##

ATGGGGCTGG CCTGGGGACT CAGTGTCCTG TTTCTGTTGC ATGTGTGTGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGCGTC TTTGACATCT TTGAACTCAC CGGGGCTGCC 
CGAAAGGGCT CTGGGCGCCG ACTAGTGAAG GGTCCTGACC CTTCCAGCCC AGCTTTCCGC 
ATTGAGGATG CCAACCTGAT CCCCCCTGTC CCTGATGACA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGAG CAGAGAAAGG TTTCCTCCTC CTGGCCTCCC TGAGGCAAAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCGT GGAGCGGAAA GACCATTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAACGGCA AGGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGGAA GCAGCAGGTG 
GTGTCAGTGG AGGAAGCCCT CCTGGCCACT GGTCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGCGAGAAGA TGGAGAATGC CGAGCTGGAC 
GTTCCCATCC AAAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAATCT CCGCATCGCA 
AAAGGAGGTG TCAATGACAA TTTTCAGGGG GTGCTGCAAA ACGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGTTGCTCCA GCTCTACCAA TGTCCTTCTC 
ACCCTGGACA ACAATGTGGT GAATGGGTCC AGCCCCGCCA TCCGCACCAA CTACATTGGC 
CACAAGACGA AGGATCTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAGC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAGT GAGCTGAGGA GGCCCCCCCT CTGCTACCAC 
AACGGAGTCC AGTATAGGAA TAACGAGGAG TGGACAGTGG ACAGCTGCAC TGAGTGTCGT 
TGTCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGCC CCATCATGCC CTGCTCTAAT 
GCCACAGTTC CTGATGGAGA ATGCTGCCCT CGGTGTTGGC CCAGCGACTC TGCTGACGAT 
GGCTGGTCCC CATGGTCTGA GTGGACCTCT TGCTCTGTGA CGTGTGGCAA TGGCATCCAG 
CAGCGTGGCC GCTCCTGTGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC CGTGCAGACT 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGACGGCGG CTGGAGCCAC 
TGGTCCCCGT GGTCGTCTTG TTCTGTCACA TGTGGAGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGTCC CCAAATGAAT GGGAAGCCAT GTGAGGGTGA AGCTCGGGAG 
ACCAAAGCCT GCCAGAGAGA CGCCTGCCCC ATCAATGGAG GCTGGGGACC CTGGTCACCA 
TGGGACATCT GTTCCGTCAC CTGTGGAGGA GGGGTACAGA GACGTAGCCG GCTCTGTAAC 
AACCCCGCAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGAGACAGA AAATCAGATC 
TGCAACAAGC AGGACTGTCC CATTGATGGA TGCCTGTCCA ATCCTTGCTT TGCTGGTGCC 
AAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGCGGTG CTTGTCCCCC TGGCTACAGT 
GGAAATGGCA TCCAGTGCGA AGATGTTGAT GAGTGCAAAG AAGTCCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACAG GTGTAAGAAC ACAGATCCCG GCTACAACTG CCTGCCCTGC 
CCACCACGTT TCACTGGCTC CCAGCCCTTT GGCCGCAGTG TGGAATACGC CACCGCCAAC 
AAACAGGTGT GCAAGCCCCG TAACCCCTGC ACAGATGGGA CGCATGACTG TAACAAGAAT 
GCCAAGTGCA ACTACCTCGG CCACTACAGC GACCCGATGT ACCGCTGCGA GTGTAAGCCT 
GGTTACGCGG GCAATGGCAT CATCTGTGGG GAGGACACGG ACCTGGACGG CTGGCCCAAC 
GAGGACCTGG TGTGCGTGGC CAATGCAACT TACCACTGCA AAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGCGATGAT 
GACGATGACA ATGATAAAAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTACG ACTATGACAG AGATGATGTG GGAGACCGCT GTGACAATTG CCCCTACAAC 
CACAACCCAG ACCAGGCCGA CACAGACAAC AATGGGGAAG GAGACGCCTG CGCGGCTGAC 
ATTGATGGGG ATGGTATCCT CAACGAACGA GACAACTGCC AGTATGTCTA CAACGTGGAC 
CAGAGAGACA CCGACATGGA TGGGGTTGGC GATCAGTGTG ACAACTGCCC CTTGGAACAC 
AATCCGGATC AGCTTGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG 
GATATTGATG AAGATGGCCA TCAGAACAAC CTGGACAACT GTCCCTATGT GCCCAACGCC 
AACCAGGCTG ACCACGACAA GGATGGCAAG GGTGATGCCT GCGACCATGA CGACGACAAT 
GATGGCATCC CTGATGACAG GGACAACTGC CGGCTTGTGC CCAATCCTGA CCAGAAGGAT 
TCTGATGGGG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCATGA CAATGTGCCA 
GACATCGATG ACATCTGTCC TGAAAATGTT GACATCAGTG AGACTGATTT CCGCCGATTC 
CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CTGGACTTGC TGTAGGTTAT 
GATGAGTTTA ACGCTGTGGA CTTCAGTGGC ACCTTTTTCA TCAACACCGA AAGGGATGAT 
GACTATGCTG GATTTGTGTT TGGCTACCAG TCCAGCAGCC GTTTCTATGT TGTGATGTGG 
AAGCAAGTCA CTCAGTCTTA CTGGGACACC AATCCCACAA GGGCTCAGGG GTACTCAGGC 
CTTTCTGTGA AGGTTGTGAA CTCCACCACA GGGCCTGGTG AGCATCTTCG GAACGCCCTG 
TGGCACACAG GAAACACCCC CGGCCAGGTG CGTACCCTGT GGCATGATCC TCGTCACATA 
GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTCAGTC ACAGACCAAA GACAGGTTTC 
ATTCGTGTGG TGATGTATGA AGGGAAGAAA ATCATGGCAG ACTCAGGACC CATCTATGAC 
AAAACCTATG CCGGTGGTAG GCTAGGGTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTT 
TCCGACCTGA AATATGAATG CAGAGATTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231465.1
CDS	181..3693
Translation	MGLAWGLSVLFLLHVCGSNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLAVERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQQVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSIFTRDLASIANLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELASELRRPPLCYHNGVQYRNNEEWTVDSCTECRCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACQRDACPINGGWGPWSPWDICSVTCGGGVQRRSRLCNNPAPQFGGKDCVGDETENQICNKQDCPIDGCLSNPCFAGAKCTSYPDGSWKCGACPPGYSGNGIQCEDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPRFTGSQPFGRSVEYATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNEDLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNCRLVPNPDQKDSDGDGRGDACKDDFDHDNVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDS

Target ORF information:

RefSeq Version	NM_001244536.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa thrombospondin 1 (THBS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244536.1

ORF Insert Sequence:

ATGGGGCTGG CCTGGGGACT CAGTGTCCTG TTTCTGTTGC ATGTGTGTGG CTCCAACCGC 
ATTCCAGAGT CTGGGGGAGA CAACAGCGTC TTTGACATCT TTGAACTCAC CGGGGCTGCC 
CGAAAGGGCT CTGGGCGCCG ACTAGTGAAG GGTCCTGACC CTTCCAGCCC AGCTTTCCGC 
ATTGAGGATG CCAACCTGAT CCCCCCTGTC CCTGATGACA AGTTCCAAGA CCTAGTGGAT 
GCTGTGCGAG CAGAGAAAGG TTTCCTCCTC CTGGCCTCCC TGAGGCAAAT GAAGAAGACC 
CGGGGCACGC TGCTGGCCGT GGAGCGGAAA GACCATTCTG GCCAGGTCTT CAGCGTGGTC 
TCCAACGGCA AGGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGGAA GCAGCAGGTG 
GTGTCAGTGG AGGAAGCCCT CCTGGCCACT GGTCAGTGGA AGAGCATCAC CCTATTTGTG 
CAGGAGGACA GGGCCCAGCT GTACATCGAC TGCGAGAAGA TGGAGAATGC CGAGCTGGAC 
GTTCCCATCC AAAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAATCT CCGCATCGCA 
AAAGGAGGTG TCAATGACAA TTTTCAGGGG GTGCTGCAAA ACGTGAGGTT TGTCTTTGGA 
ACCACACCAG AAGACATCCT CAGGAACAAA GGTTGCTCCA GCTCTACCAA TGTCCTTCTC 
ACCCTGGACA ACAATGTGGT GAATGGGTCC AGCCCCGCCA TCCGCACCAA CTACATTGGC 
CACAAGACGA AGGATCTGCA AGCCATCTGC GGCATCTCCT GTGATGAGCT GTCCAGCATG 
GTCCTGGAGC TCAGGGGCCT GCGCACCATT GTGACCACGC TGCAGGACAG CATCCGCAAA 
GTGACTGAAG AGAACAAAGA GTTGGCCAGT GAGCTGAGGA GGCCCCCCCT CTGCTACCAC 
AACGGAGTCC AGTATAGGAA TAACGAGGAG TGGACAGTGG ACAGCTGCAC TGAGTGTCGT 
TGTCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGCC CCATCATGCC CTGCTCTAAT 
GCCACAGTTC CTGATGGAGA ATGCTGCCCT CGGTGTTGGC CCAGCGACTC TGCTGACGAT 
GGCTGGTCCC CATGGTCTGA GTGGACCTCT TGCTCTGTGA CGTGTGGCAA TGGCATCCAG 
CAGCGTGGCC GCTCCTGTGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC CGTGCAGACT 
CGGACCTGCC ACATTCAGGA GTGTGACAAG AGATTTAAAC AGGACGGCGG CTGGAGCCAC 
TGGTCCCCGT GGTCGTCTTG TTCTGTCACA TGTGGAGATG GTGTGATCAC AAGGATCCGG 
CTCTGCAACT CTCCCAGTCC CCAAATGAAT GGGAAGCCAT GTGAGGGTGA AGCTCGGGAG 
ACCAAAGCCT GCCAGAGAGA CGCCTGCCCC ATCAATGGAG GCTGGGGACC CTGGTCACCA 
TGGGACATCT GTTCCGTCAC CTGTGGAGGA GGGGTACAGA GACGTAGCCG GCTCTGTAAC 
AACCCCGCAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGAGACAGA AAATCAGATC 
TGCAACAAGC AGGACTGTCC CATTGATGGA TGCCTGTCCA ATCCTTGCTT TGCTGGTGCC 
AAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGCGGTG CTTGTCCCCC TGGCTACAGT 
GGAAATGGCA TCCAGTGCGA AGATGTTGAT GAGTGCAAAG AAGTCCCTGA TGCCTGCTTC 
AACCACAATG GAGAGCACAG GTGTAAGAAC ACAGATCCCG GCTACAACTG CCTGCCCTGC 
CCACCACGTT TCACTGGCTC CCAGCCCTTT GGCCGCAGTG TGGAATACGC CACCGCCAAC 
AAACAGGTGT GCAAGCCCCG TAACCCCTGC ACAGATGGGA CGCATGACTG TAACAAGAAT 
GCCAAGTGCA ACTACCTCGG CCACTACAGC GACCCGATGT ACCGCTGCGA GTGTAAGCCT 
GGTTACGCGG GCAATGGCAT CATCTGTGGG GAGGACACGG ACCTGGACGG CTGGCCCAAC 
GAGGACCTGG TGTGCGTGGC CAATGCAACT TACCACTGCA AAAAGGATAA TTGCCCCAAC 
CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGCGATGAT 
GACGATGACA ATGATAAAAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA 
GCCCAGTACG ACTATGACAG AGATGATGTG GGAGACCGCT GTGACAATTG CCCCTACAAC 
CACAACCCAG ACCAGGCCGA CACAGACAAC AATGGGGAAG GAGACGCCTG CGCGGCTGAC 
ATTGATGGGG ATGGTATCCT CAACGAACGA GACAACTGCC AGTATGTCTA CAACGTGGAC 
CAGAGAGACA CCGACATGGA TGGGGTTGGC GATCAGTGTG ACAACTGCCC CTTGGAACAC 
AATCCGGATC AGCTTGACTC TGACTCAGAC CGCATTGGAG ATACCTGTGA CAACAATCAG 
GATATTGATG AAGATGGCCA TCAGAACAAC CTGGACAACT GTCCCTATGT GCCCAACGCC 
AACCAGGCTG ACCACGACAA GGATGGCAAG GGTGATGCCT GCGACCATGA CGACGACAAT 
GATGGCATCC CTGATGACAG GGACAACTGC CGGCTTGTGC CCAATCCTGA CCAGAAGGAT 
TCTGATGGGG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCATGA CAATGTGCCA 
GACATCGATG ACATCTGTCC TGAAAATGTT GACATCAGTG AGACTGATTT CCGCCGATTC 
CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC 
CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CTGGACTTGC TGTAGGTTAT 
GATGAGTTTA ACGCTGTGGA CTTCAGTGGC ACCTTTTTCA TCAACACCGA AAGGGATGAT 
GACTATGCTG GATTTGTGTT TGGCTACCAG TCCAGCAGCC GTTTCTATGT TGTGATGTGG 
AAGCAAGTCA CTCAGTCTTA CTGGGACACC AATCCCACAA GGGCTCAGGG GTACTCAGGC 
CTTTCTGTGA AGGTTGTGAA CTCCACCACA GGGCCTGGTG AGCATCTTCG GAACGCCCTG 
TGGCACACAG GAAACACCCC CGGCCAGGTG CGTACCCTGT GGCATGATCC TCGTCACATA 
GGCTGGAAAG ATTTCACTGC CTACAGATGG CGTCTCAGTC ACAGACCAAA GACAGGTTTC 
ATTCGTGTGG TGATGTATGA AGGGAAGAAA ATCATGGCAG ACTCAGGACC CATCTATGAC 
AAAACCTATG CCGGTGGTAG GCTAGGGTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTT 
TCCGACCTGA AATATGAATG CAGAGATTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MiR-222 inhibits apoptosis in porcine follicular granulosa cells by targeting the THBS1 gene.
Animal science journal = Nihon chikusan Gakkaiho90(6)719-727(2019 Jun)
Zhu W,Yang M,Shang J,Xu Y,Wang Y,Tao Q,Zhang L,Ding Y,Chen Y,Zhao D,Wang C,Chu M,Yin Z,Zhang X

Glucose regulation of thrombospondin and its role in the modulation of smooth muscle cell proliferation.
Experimental diabetes research2010(2010)
Maile LA,Allen LB,Hanzaker CF,Gollahon KA,Dunbar P,Clemmons DR

Thrombospondin-1 mRNA expression in experimental kidney transplantation with heart-beating and non-heart-beating donors.
Journal of nephrology20(5)588-95(2007 Sep-Oct)
Lario S,Bescós M,Campos B,Mur C,Luque P,Alvarez R,Campistol JM

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THBS1 cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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