DDX58 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX58
Entrez Gene ID	396723
Full Name	DExD/H-box helicase 58
Synonyms	RHIV-1,RIG-1,RIG-I,RLR-1
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 10 Map Location: 10\|10

mRNA and Protein(s)

mRNA	Protein	Name
XM_021064056.1	XP_020919715.1	probable ATP-dependent RNA helicase DDX58 isoform X2
XM_021064055.1	XP_020919714.1	probable ATP-dependent RNA helicase DDX58 isoform X2
XM_021064058.1	XP_020919717.1	probable ATP-dependent RNA helicase DDX58 isoform X2
XM_021064057.1	XP_020919716.1	probable ATP-dependent RNA helicase DDX58 isoform X2
XM_021064054.1	XP_020919713.1	probable ATP-dependent RNA helicase DDX58 isoform X1
NM_213804.2	NP_998969.2	probable ATP-dependent RNA helicase DDX58

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Nucleocapsid protein of porcine reproductive and respiratory syndrome virus antagonizes the antiviral activity of TRIM25 by interfering with TRIM25-mediated RIG-I ubiquitination.
Zhao K, Li LW, Jiang YF, Gao F, Zhang YJ, Zhao WY, Li GX, Yu LX, Zhou YJ, Tong GZ
Veterinary microbiology233140-146(2019 Jun)

RIG-I is responsible for activation of type I interferon pathway in Seneca Valley virus-infected porcine cells to suppress viral replication.
Li P, Zhang X, Cao W, Yang F, Du X, Shi Z, Zhang M, Liu X, Zhu Z, Zheng H
Virology journal15(1)162(2018 10)

Porcine circovirus 2 infection induces IFNβ expression through increased expression of genes involved in RIG-I and IRF7 signaling pathways.
Dvorak CMT, Puvanendiran S, Murtaugh MP
Virus research25338-47(2018 07)

Identification of the RNA Pseudoknot within the 3' End of the Porcine Reproductive and Respiratory Syndrome Virus Genome as a Pathogen-Associated Molecular Pattern To Activate Antiviral Signaling via RIG-I and Toll-Like Receptor 3.
Xie S, Chen XX, Qiao S, Li R, Sun Y, Xia S, Wang LJ, Luo X, Deng R, Zhou EM, Zhang GP
Journal of virology92(12)(2018 06)

Interactions between the influenza A virus RNA polymerase components and retinoic acid-inducible gene I.
Li W, Chen H, Sutton T, Obadan A, Perez DR
Journal of virology88(18)10432-47(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DDX58 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX58 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe00492	NM_213804.2 Latest version!	Sus scrofa DExD/H-box helicase 58 (DDX58), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSe231726	XM_021064054.1 Latest version!	Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSe231727	XM_021064057.1 Latest version!	Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSe231727	XM_021064055.1 Latest version!	Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSe231727	XM_021064056.1 Latest version!	Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSe231727	XM_021064058.1 Latest version!	Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX58 ( NM_213804.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_998969.2 Sus scrofa DExD/H-box helicase 58 (DDX58), mRNA.

Price & Availability↑

CloneID	OSe00492
Clone ID Related Accession (Same CDS sequence)	NM_213804.2
Accession Version	NM_213804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX58
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB426546.1. On Nov 5, 2011 this sequence version replaced NM_213804.1. ##Evidence-Data-START## Transcript exon combination :: AB426546.1, EU126659.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3179999, SAMEA3180005 [ECO:0000348] ##Evidence-Data-END##

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTCTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACCAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTT 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGACG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGAAGCAC CTCATACTTA CAGCCCACTG 
AAGCCAAGAA ATTACCAACT GGAGCTTGCT TTACCTGCTC AGAATGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCTC TTCTTATATG TGAACATCAT 
CTTAAGAAAT TTCCACAAGG ACGAAAGGGG AAGGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAACATTTTG AAAGACTTGG GTACAAAGTT 
GCAGGCATTT CTGGAGCAAC GTCTGACACT GTCTGTGTGG AACAGATTGT TGAGAACAGC 
GACATCATCA TCTTAACGCC ACAGATTCTT GTGAACTGTC TTACCAATGG GACAATTCCA 
TCACTCTCTG TGTTTACTTT GATGATATTT GATGAATGCC ACAACACCAG CAAACAGCAT 
CCTTATAATG TCATCATGTT TAGTTATCTA GATCGGAAAC TTGGAGGATC TTCAGACTCA 
CTGCCCCAGG TCATTGGACT GACTGCCTCG GTTGGCGTTG GGGATGCAAA AAACAAGGCT 
GAAGCCACAG AATACATCTG CAAATTGTGT GCTTCTCTTG ACACTTCGGT CATAGCAACA 
GTCAGAGACA ACTTGGAAGA ACTAGAGGAA GTTGTGTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT TACGGACCAC TGACAGATTT AAGTGCATCA TATCGCAGCT GATGATGGAG 
ATAGAGAGTC TGGCAAAGAG CATCTTTGAA GAACTTGGTA CCATAACTCT TGGAGGCTTA 
TTTCAAATTC AAAATAGTAA TTTCGGAACA CAGAAATATG AGCAGTGGAT TGTCAAGGTT 
CAGAAAGAGT GTGCGGTGTT TCAGATGCCA GACAAAGACA AAGAAAGCAG GATTTGTAAA 
GCACTGTTTT CGTACATGTC ACATTTGCGG ATATACAACG ATGCCCTTAT TATCAATGAA 
CATGCCCGAA TGAAAGATGC TTTGGATTAC TTGAAAGACT TCTTCAGAAA TATCCGAGCA 
GCAGGCTTTG ATGAGATTGA GCAAGATCTC ACTCAGAGAT TTGAAGAAAA GCTGCAAGAA 
CTAGAAAGCA TTTCCATAGA TCCCAGCAAC GAGAACCCTA AACTCAGAGA CCTCTGCTTC 
ATCTTGCAAG AAGAGTACCA CTTAAACCCA GAGACCAGAA CCATTCTCTT TGTGAAAACC 
AGAGCCCTTG TGGATGCTTT AAAGAAATGG ATTAAAGAAA ATCCTAAGCT CAGCTTTCTG 
AAACCTAGCA TATTGACTGG ACGTGGCAAA ACGAATCAGA ACATAGGGAT GACCCTCCCA 
GCCCAGAAGT GTGTACTGGA TACCTTCAGA ACTGACAAGG ATAACAAGAT TCTGATCACC 
ACCTCGGTGG CAGATGAAGG CATCGACATT GCTCAGTGCA ATCTGGTCAT CCTATATGAG 
TATGTGGGCA ACGTCATCAA AATGATCCAA ACCAGAGGCA GAGGAAGAGC AAGAGGGAGC 
AAGTGTTTCC TTCTGACTGC TAACGCTGAT CTGATCGACA AAGAGAAAAT GAACATGTAC 
AAAGAAGAAA TGATGAATGG GGCCATTTTA ATCCTGCAGA CATGGGATGA AGCAGTATTT 
AAAGAAAAGA TTCACCAGAT ACAGATTCGT GAAAAAATCA TCAGGGATAA TCAAGGAAAA 
CCAGAACCTG TGCCTGATAA GAAAACTAAA AAACTACTCT GCAAAAAGTG CAAAGCCTTT 
GCATGTTATA CAGCTGATAT AAGAATGGTG GAGAAATGCC ATTTCACTGT GGTTGGAGAT 
GCTTTCAGGG AGCGCTTTGT GAGTAAACTG CACCCCAAAC CAAAGAGTTT TGGGAATATT 
GAAAAGAGAG CAAAGATATA TTGTGCTAGG CCAGACTGCA GCCATGACTG GGGAATCTAT 
GTGAGGTATA AGGCATTTGA GATGCCATTT ATAAAAATCG AAAGTTTTGT GGTGGAGGAT 
ATTGCAACTG GAGTTCAGAC CGTGCATGCC AAGTGGAAGG ACTTTAATTT TGAGAAGCTA 
TCATTTGATG CTGCAGAAAT GGCCGGTGGA GCTCAGGACC TGGGTCTTCA GGGAATGGGC 
AACCTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998969.2
CDS	1..2832
Translation	MTAEQRRNLHAFGDYVRKTLDPTFILSYMAPWFRDDEVQHIQAEKNNKGPTEAASLFLQFLLELQEEGWFRGFLDALNQAGYSGLCEAIESWDFQKIEKLEEYRSLLRRLQPEFKTTINPKDILPEIAECLISQECEEILQICSSKGLMAGAEKMVECLLRSDKENWPKTLKLALEKEESRFSELWMVDKGAEDVKMKDLEDDEMKTCDVQIFYKEEPENQNLSQNSCSSSEAPHTYSPLKPRNYQLELALPAQNGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGRKGKVVFFAIQLPVYEQQKSVFSKHFERLGYKVAGISGATSDTVCVEQIVENSDIIILTPQILVNCLTNGTIPSLSVFTLMIFDECHNTSKQHPYNVIMFSYLDRKLGGSSDSLPQVIGLTASVGVGDAKNKAEATEYICKLCASLDTSVIATVRDNLEELEEVVYKPQKFFRKVELRTTDRFKCIISQLMMEIESLAKSIFEELGTITLGGLFQIQNSNFGTQKYEQWIVKVQKECAVFQMPDKDKESRICKALFSYMSHLRIYNDALIINEHARMKDALDYLKDFFRNIRAAGFDEIEQDLTQRFEEKLQELESISIDPSNENPKLRDLCFILQEEYHLNPETRTILFVKTRALVDALKKWIKENPKLSFLKPSILTGRGKTNQNIGMTLPAQKCVLDTFRTDKDNKILITTSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTANADLIDKEKMNMYKEEMMNGAILILQTWDEAVFKEKIHQIQIREKIIRDNQGKPEPVPDKKTKKLLCKKCKAFACYTADIRMVEKCHFTVVGDAFRERFVSKLHPKPKSFGNIEKRAKIYCARPDCSHDWGIYVRYKAFEMPFIKIESFVVEDIATGVQTVHAKWKDFNFEKLSFDAAEMAGGAQDLGLQGMGNLE

Target ORF information:

RefSeq Version	NM_213804.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa DExD/H-box helicase 58 (DDX58), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213804.2

ORF Insert Sequence:

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTCTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACCAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTT 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGACG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGAAGCAC CTCATACTTA CAGCCCACTG 
AAGCCAAGAA ATTACCAACT GGAGCTTGCT TTACCTGCTC AGAATGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCTC TTCTTATATG TGAACATCAT 
CTTAAGAAAT TTCCACAAGG ACGAAAGGGG AAGGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAACATTTTG AAAGACTTGG GTACAAAGTT 
GCAGGCATTT CTGGAGCAAC GTCTGACACT GTCTGTGTGG AACAGATTGT TGAGAACAGC 
GACATCATCA TCTTAACGCC ACAGATTCTT GTGAACTGTC TTACCAATGG GACAATTCCA 
TCACTCTCTG TGTTTACTTT GATGATATTT GATGAATGCC ACAACACCAG CAAACAGCAT 
CCTTATAATG TCATCATGTT TAGTTATCTA GATCGGAAAC TTGGAGGATC TTCAGACTCA 
CTGCCCCAGG TCATTGGACT GACTGCCTCG GTTGGCGTTG GGGATGCAAA AAACAAGGCT 
GAAGCCACAG AATACATCTG CAAATTGTGT GCTTCTCTTG ACACTTCGGT CATAGCAACA 
GTCAGAGACA ACTTGGAAGA ACTAGAGGAA GTTGTGTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT TACGGACCAC TGACAGATTT AAGTGCATCA TATCGCAGCT GATGATGGAG 
ATAGAGAGTC TGGCAAAGAG CATCTTTGAA GAACTTGGTA CCATAACTCT TGGAGGCTTA 
TTTCAAATTC AAAATAGTAA TTTCGGAACA CAGAAATATG AGCAGTGGAT TGTCAAGGTT 
CAGAAAGAGT GTGCGGTGTT TCAGATGCCA GACAAAGACA AAGAAAGCAG GATTTGTAAA 
GCACTGTTTT CGTACATGTC ACATTTGCGG ATATACAACG ATGCCCTTAT TATCAATGAA 
CATGCCCGAA TGAAAGATGC TTTGGATTAC TTGAAAGACT TCTTCAGAAA TATCCGAGCA 
GCAGGCTTTG ATGAGATTGA GCAAGATCTC ACTCAGAGAT TTGAAGAAAA GCTGCAAGAA 
CTAGAAAGCA TTTCCATAGA TCCCAGCAAC GAGAACCCTA AACTCAGAGA CCTCTGCTTC 
ATCTTGCAAG AAGAGTACCA CTTAAACCCA GAGACCAGAA CCATTCTCTT TGTGAAAACC 
AGAGCCCTTG TGGATGCTTT AAAGAAATGG ATTAAAGAAA ATCCTAAGCT CAGCTTTCTG 
AAACCTAGCA TATTGACTGG ACGTGGCAAA ACGAATCAGA ACATAGGGAT GACCCTCCCA 
GCCCAGAAGT GTGTACTGGA TACCTTCAGA ACTGACAAGG ATAACAAGAT TCTGATCACC 
ACCTCGGTGG CAGATGAAGG CATCGACATT GCTCAGTGCA ATCTGGTCAT CCTATATGAG 
TATGTGGGCA ACGTCATCAA AATGATCCAA ACCAGAGGCA GAGGAAGAGC AAGAGGGAGC 
AAGTGTTTCC TTCTGACTGC TAACGCTGAT CTGATCGACA AAGAGAAAAT GAACATGTAC 
AAAGAAGAAA TGATGAATGG GGCCATTTTA ATCCTGCAGA CATGGGATGA AGCAGTATTT 
AAAGAAAAGA TTCACCAGAT ACAGATTCGT GAAAAAATCA TCAGGGATAA TCAAGGAAAA 
CCAGAACCTG TGCCTGATAA GAAAACTAAA AAACTACTCT GCAAAAAGTG CAAAGCCTTT 
GCATGTTATA CAGCTGATAT AAGAATGGTG GAGAAATGCC ATTTCACTGT GGTTGGAGAT 
GCTTTCAGGG AGCGCTTTGT GAGTAAACTG CACCCCAAAC CAAAGAGTTT TGGGAATATT 
GAAAAGAGAG CAAAGATATA TTGTGCTAGG CCAGACTGCA GCCATGACTG GGGAATCTAT 
GTGAGGTATA AGGCATTTGA GATGCCATTT ATAAAAATCG AAAGTTTTGT GGTGGAGGAT 
ATTGCAACTG GAGTTCAGAC CGTGCATGCC AAGTGGAAGG ACTTTAATTT TGAGAAGCTA 
TCATTTGATG CTGCAGAAAT GGCCGGTGGA GCTCAGGACC TGGGTCTTCA GGGAATGGGC 
AACCTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX58 ( XM_021064054.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020919713.1 Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe231726
Clone ID Related Accession (Same CDS sequence)	XM_021064054.1
Accession Version	XM_021064054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX58 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010452.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTGTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACTAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTA 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGCACCTC ATACTTACAG CCCACTGAAG 
CCAAGAAAGT ACCAACTGGA GCTTGCTTTA CCTGCTCAGA ATGGAAAAAA CACAATAATA 
TGTGCTCCTA CTGGTTGTGG AAAAACCTTT GTTTCTCTTC TTATATGTGA ACATCATCTT 
AAGAAATTTC CACGAGGACG AAAGGGGAAG GTTGTCTTTT TTGCTATTCA ACTCCCAGTG 
TATGAGCAGC AGAAATCTGT GTTCTCAAAA CATTTTGAAA GACTTGGGTA CAAAGTTGCA 
GGCATTTCTG GAGCAACGTC TGACACTGTC TGTGTGGAAC AGATTGTTGA GAACAACGAC 
ATCATCATCT TAACACCACA GATTCTTGTG AACTGTCTTA CCAATGGGAC AATTCCATCA 
CTCTCTGTGT TTACTTTGAT GATATTTGAT GAATGCCACA ACACCAGCAA ACAGCATCCT 
TATAATGTCA TCATGTTTAA TTATCTAGAT CGGAAACTTG GAGGATCTTC AGACTCACTG 
CCCCAGGTCA TTGGACTGAC TGCCTCGGTT GGCGTTGGGG ATGCAAAAAA CAAGGCTGAA 
GCCACAGAAT ACATCTGCAA ATTGTGTGCT TCTCTTGACA CTTCGGTCAT AGCAACAGTC 
AGAGACAACT TGGAAGAACT AGAGGAAGTT GTGTATAAGC CCCAGAAGTT TTTCAGGAAA 
GTGGAATTAC GGACCACTGA CAGATTTAAG TGCATCATAT CACAGCTGAT GATGGAGATA 
GAGAGTCTGG CAAAGAGCAT CTTTGAAGAA CTTGGTACCA TAACTCTTGG AGGCTTATTT 
CAAATTCAAA ATAGTAATTT CGGAACACAG AAATATGAGC AGTGGATTGT CAAGGTTCAG 
AAAGAATGTG CGGTGTTTCA GATGCCAGAC AAAGACAAAG AAAGCAGGAT TTGTAAAGCA 
CTGTTTTCGT ACATGTCACA TTTGCGGATA TACAACGATG CCCTTATTAT CAATGAACAT 
GCCCGAATGA AAGATGCTTT GGATTACTTG AAAGACTTCT TCAGAAATAT CCGAGCAGCA 
GGCTTTGATG AGATTGAGCA AGATCTCACT CAGAGATTTG AAGAAAAGCT GCAAGAACTA 
GAAAGCATTT CCATAGATCC CAGCAACGAG AACCCTAAAC TCAGAGACCT CTGCTTCATC 
TTGCAAGAAG AGTACCACTT AAACCCAGAG ACCAGAACCA TTCTCTTTGT GAAAACCAGA 
GCCCTTGTGG ATGCTTTAAA GAAATGGATT AAAGAAAATC CTAAGCTCAG CTTTCTGAAA 
CCTAGCATAT TGACTGGACG TGGCAAAACG AATCAGAACA TAGGGATGAC CCTCCCAGCC 
CAGAAGTGTG TACTGGATAC CTTCAGAACT GACAAGGATA ACAAGATTCT GATCACCACC 
TCGGTGGCAG ATGAAGGCAT CGACATTGCT CAGTGCAATC TGGTCATCCT ATATGAGTAT 
GTGGGCAACG TCATCAAAAT GATCCAAACC AGAGGCAGAG GAAGAGCAAG AGGGAGCAAG 
TGTTTCCTTC TGACTGCTAA CGCTGATCTG ATCGACAAAG AGAAAATGAA CATGTACAAA 
GAAGAAATGA TGAATGGGGC CATTTTAATC CTGCAGACAT GGGATGAAGC AGTATTTAAA 
GAAAAGATTC ACCAGATACA GATTCGTGAA AAAATCATCA GGGATAATCA AGGAAAACCA 
GAACCTGTGC CTGATAAGAA AACTAAAAAA CTACTCTGCA AAAAGTGCAA AGCCTTTGCA 
TGTTATACAG CTGATATAAG AATGGTGGAG AATTGCCATT TCACTGTGGT TGGAGATGCT 
TTCAGGGAGC GCTTTGTGAG TAAACTGCAC CCCAAACCAA AGAGTTTTGG GAATATTGAA 
AAGAGAGCAA AGATATATTG TGCTAGGCCA GACTGCAGCC ATGACTGGGG AATCTATGTG 
AGGTATAAGG CATTTGAGAT GCCATTTATA AAAATCGAAA GTTTTGTGGT GGAGGATATT 
GCAACTGGAG TTCAGACCGT GCATGCCAAG TGGAAGGACT TTAATTTTGA GAAGCTATCA 
TTTGATGCTG CAGAAATGGC CGGTGGAGCT CAGGACCTGG GTCTTCAGGG AATGGGCAAC 
CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020919713.1
CDS	82..2910
Translation	MTAEQRRNLHAFGDYVRKTLDPTFILSYMAPWFRDDEVQHIQAEKNNKGPTEAASLFLQFLLELQEEGWFRGFLDALNQAGYCGLCEAIESWDFQKIEKLEEYRSLLRRLQPEFKTTINPKDILPEIAECLISQECEEILQICSSKGLMAGAEKMVECLLRSDKENWPKTLKLALEKEESRFSELWMVDKGAEDVKMKDLEDDEMKTCDVQIFYKEEPENQNLSQNSCSSSAPHTYSPLKPRKYQLELALPAQNGKNTIICAPTGCGKTFVSLLICEHHLKKFPRGRKGKVVFFAIQLPVYEQQKSVFSKHFERLGYKVAGISGATSDTVCVEQIVENNDIIILTPQILVNCLTNGTIPSLSVFTLMIFDECHNTSKQHPYNVIMFNYLDRKLGGSSDSLPQVIGLTASVGVGDAKNKAEATEYICKLCASLDTSVIATVRDNLEELEEVVYKPQKFFRKVELRTTDRFKCIISQLMMEIESLAKSIFEELGTITLGGLFQIQNSNFGTQKYEQWIVKVQKECAVFQMPDKDKESRICKALFSYMSHLRIYNDALIINEHARMKDALDYLKDFFRNIRAAGFDEIEQDLTQRFEEKLQELESISIDPSNENPKLRDLCFILQEEYHLNPETRTILFVKTRALVDALKKWIKENPKLSFLKPSILTGRGKTNQNIGMTLPAQKCVLDTFRTDKDNKILITTSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTANADLIDKEKMNMYKEEMMNGAILILQTWDEAVFKEKIHQIQIREKIIRDNQGKPEPVPDKKTKKLLCKKCKAFACYTADIRMVENCHFTVVGDAFRERFVSKLHPKPKSFGNIEKRAKIYCARPDCSHDWGIYVRYKAFEMPFIKIESFVVEDIATGVQTVHAKWKDFNFEKLSFDAAEMAGGAQDLGLQGMGNLE

Target ORF information:

RefSeq Version	XM_021064054.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021064054.1

ORF Insert Sequence:

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTGTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACTAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTA 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGCACCTC ATACTTACAG CCCACTGAAG 
CCAAGAAAGT ACCAACTGGA GCTTGCTTTA CCTGCTCAGA ATGGAAAAAA CACAATAATA 
TGTGCTCCTA CTGGTTGTGG AAAAACCTTT GTTTCTCTTC TTATATGTGA ACATCATCTT 
AAGAAATTTC CACGAGGACG AAAGGGGAAG GTTGTCTTTT TTGCTATTCA ACTCCCAGTG 
TATGAGCAGC AGAAATCTGT GTTCTCAAAA CATTTTGAAA GACTTGGGTA CAAAGTTGCA 
GGCATTTCTG GAGCAACGTC TGACACTGTC TGTGTGGAAC AGATTGTTGA GAACAACGAC 
ATCATCATCT TAACACCACA GATTCTTGTG AACTGTCTTA CCAATGGGAC AATTCCATCA 
CTCTCTGTGT TTACTTTGAT GATATTTGAT GAATGCCACA ACACCAGCAA ACAGCATCCT 
TATAATGTCA TCATGTTTAA TTATCTAGAT CGGAAACTTG GAGGATCTTC AGACTCACTG 
CCCCAGGTCA TTGGACTGAC TGCCTCGGTT GGCGTTGGGG ATGCAAAAAA CAAGGCTGAA 
GCCACAGAAT ACATCTGCAA ATTGTGTGCT TCTCTTGACA CTTCGGTCAT AGCAACAGTC 
AGAGACAACT TGGAAGAACT AGAGGAAGTT GTGTATAAGC CCCAGAAGTT TTTCAGGAAA 
GTGGAATTAC GGACCACTGA CAGATTTAAG TGCATCATAT CACAGCTGAT GATGGAGATA 
GAGAGTCTGG CAAAGAGCAT CTTTGAAGAA CTTGGTACCA TAACTCTTGG AGGCTTATTT 
CAAATTCAAA ATAGTAATTT CGGAACACAG AAATATGAGC AGTGGATTGT CAAGGTTCAG 
AAAGAATGTG CGGTGTTTCA GATGCCAGAC AAAGACAAAG AAAGCAGGAT TTGTAAAGCA 
CTGTTTTCGT ACATGTCACA TTTGCGGATA TACAACGATG CCCTTATTAT CAATGAACAT 
GCCCGAATGA AAGATGCTTT GGATTACTTG AAAGACTTCT TCAGAAATAT CCGAGCAGCA 
GGCTTTGATG AGATTGAGCA AGATCTCACT CAGAGATTTG AAGAAAAGCT GCAAGAACTA 
GAAAGCATTT CCATAGATCC CAGCAACGAG AACCCTAAAC TCAGAGACCT CTGCTTCATC 
TTGCAAGAAG AGTACCACTT AAACCCAGAG ACCAGAACCA TTCTCTTTGT GAAAACCAGA 
GCCCTTGTGG ATGCTTTAAA GAAATGGATT AAAGAAAATC CTAAGCTCAG CTTTCTGAAA 
CCTAGCATAT TGACTGGACG TGGCAAAACG AATCAGAACA TAGGGATGAC CCTCCCAGCC 
CAGAAGTGTG TACTGGATAC CTTCAGAACT GACAAGGATA ACAAGATTCT GATCACCACC 
TCGGTGGCAG ATGAAGGCAT CGACATTGCT CAGTGCAATC TGGTCATCCT ATATGAGTAT 
GTGGGCAACG TCATCAAAAT GATCCAAACC AGAGGCAGAG GAAGAGCAAG AGGGAGCAAG 
TGTTTCCTTC TGACTGCTAA CGCTGATCTG ATCGACAAAG AGAAAATGAA CATGTACAAA 
GAAGAAATGA TGAATGGGGC CATTTTAATC CTGCAGACAT GGGATGAAGC AGTATTTAAA 
GAAAAGATTC ACCAGATACA GATTCGTGAA AAAATCATCA GGGATAATCA AGGAAAACCA 
GAACCTGTGC CTGATAAGAA AACTAAAAAA CTACTCTGCA AAAAGTGCAA AGCCTTTGCA 
TGTTATACAG CTGATATAAG AATGGTGGAG AATTGCCATT TCACTGTGGT TGGAGATGCT 
TTCAGGGAGC GCTTTGTGAG TAAACTGCAC CCCAAACCAA AGAGTTTTGG GAATATTGAA 
AAGAGAGCAA AGATATATTG TGCTAGGCCA GACTGCAGCC ATGACTGGGG AATCTATGTG 
AGGTATAAGG CATTTGAGAT GCCATTTATA AAAATCGAAA GTTTTGTGGT GGAGGATATT 
GCAACTGGAG TTCAGACCGT GCATGCCAAG TGGAAGGACT TTAATTTTGA GAAGCTATCA 
TTTGATGCTG CAGAAATGGC CGGTGGAGCT CAGGACCTGG GTCTTCAGGG AATGGGCAAC 
CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX58 ( XM_021064056.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020919715.1 Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSe231727
Clone ID Related Accession (Same CDS sequence)	XM_021064056.1 , XM_021064055.1 , XM_021064058.1 , XM_021064057.1
Accession Version	XM_021064056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX58 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010452.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTGTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACTAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTA 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGAAGCAC CTCATACTTA CAGCCCACTG 
AAGCCAAGAA AGTACCAACT GGAGCTTGCT TTACCTGCTC AGAATGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCTC TTCTTATATG TGAACATCAT 
CTTAAGAAAT TTCCACGAGG ACGAAAGGGG AAGGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAACATTTTG AAAGACTTGG GTACAAAGTT 
GCAGGCATTT CTGGAGCAAC GTCTGACACT GTCTGTGTGG AACAGATTGT TGAGAACAAC 
GACATCATCA TCTTAACACC ACAGATTCTT GTGAACTGTC TTACCAATGG GACAATTCCA 
TCACTCTCTG TGTTTACTTT GATGATATTT GATGAATGCC ACAACACCAG CAAACAGCAT 
CCTTATAATG TCATCATGTT TAATTATCTA GATCGGAAAC TTGGAGGATC TTCAGACTCA 
CTGCCCCAGG TCATTGGACT GACTGCCTCG GTTGGCGTTG GGGATGCAAA AAACAAGGCT 
GAAGCCACAG AATACATCTG CAAATTGTGT GCTTCTCTTG ACACTTCGGT CATAGCAACA 
GTCAGAGACA ACTTGGAAGA ACTAGAGGAA GTTGTGTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT TACGGACCAC TGACAGATTT AAGTGCATCA TATCACAGCT GATGATGGAG 
ATAGAGAGTC TGGCAAAGAG CATCTTTGAA GAACTTGGTA CCATAACTCT TGGAGGCTTA 
TTTCAAATTC AAAATAGTAA TTTCGGAACA CAGAAATATG AGCAGTGGAT TGTCAAGGTT 
CAGAAAGAAT GTGCGGTGTT TCAGATGCCA GACAAAGACA AAGAAAGCAG GATTTGTAAA 
GCACTGTTTT CGTACATGTC ACATTTGCGG ATATACAACG ATGCCCTTAT TATCAATGAA 
CATGCCCGAA TGAAAGATGC TTTGGATTAC TTGAAAGACT TCTTCAGAAA TATCCGAGCA 
GCAGGCTTTG ATGAGATTGA GCAAGATCTC ACTCAGAGAT TTGAAGAAAA GCTGCAAGAA 
CTAGAAAGCA TTTCCATAGA TCCCAGCAAC GAGAACCCTA AACTCAGAGA CCTCTGCTTC 
ATCTTGCAAG AAGAGTACCA CTTAAACCCA GAGACCAGAA CCATTCTCTT TGTGAAAACC 
AGAGCCCTTG TGGATGCTTT AAAGAAATGG ATTAAAGAAA ATCCTAAGCT CAGCTTTCTG 
AAACCTAGCA TATTGACTGG ACGTGGCAAA ACGAATCAGA ACATAGGGAT GACCCTCCCA 
GCCCAGAAGT GTGTACTGGA TACCTTCAGA ACTGACAAGG ATAACAAGAT TCTGATCACC 
ACCTCGGTGG CAGATGAAGG CATCGACATT GCTCAGTGCA ATCTGGTCAT CCTATATGAG 
TATGTGGGCA ACGTCATCAA AATGATCCAA ACCAGAGGCA GAGGAAGAGC AAGAGGGAGC 
AAGTGTTTCC TTCTGACTGC TAACGCTGAT CTGATCGACA AAGAGAAAAT GAACATGTAC 
AAAGAAGAAA TGATGAATGG GGCCATTTTA ATCCTGCAGA CATGGGATGA AGCAGTATTT 
AAAGAAAAGA TTCACCAGAT ACAGATTCGT GAAAAAATCA TCAGGGATAA TCAAGGAAAA 
CCAGAACCTG TGCCTGATAA GAAAACTAAA AAACTACTCT GCAAAAAGTG CAAAGCCTTT 
GCATGTTATA CAGCTGATAT AAGAATGGTG GAGGAACTCT GTGTATTGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020919715.1
CDS	82..2595
Translation	MTAEQRRNLHAFGDYVRKTLDPTFILSYMAPWFRDDEVQHIQAEKNNKGPTEAASLFLQFLLELQEEGWFRGFLDALNQAGYCGLCEAIESWDFQKIEKLEEYRSLLRRLQPEFKTTINPKDILPEIAECLISQECEEILQICSSKGLMAGAEKMVECLLRSDKENWPKTLKLALEKEESRFSELWMVDKGAEDVKMKDLEDDEMKTCDVQIFYKEEPENQNLSQNSCSSSEAPHTYSPLKPRKYQLELALPAQNGKNTIICAPTGCGKTFVSLLICEHHLKKFPRGRKGKVVFFAIQLPVYEQQKSVFSKHFERLGYKVAGISGATSDTVCVEQIVENNDIIILTPQILVNCLTNGTIPSLSVFTLMIFDECHNTSKQHPYNVIMFNYLDRKLGGSSDSLPQVIGLTASVGVGDAKNKAEATEYICKLCASLDTSVIATVRDNLEELEEVVYKPQKFFRKVELRTTDRFKCIISQLMMEIESLAKSIFEELGTITLGGLFQIQNSNFGTQKYEQWIVKVQKECAVFQMPDKDKESRICKALFSYMSHLRIYNDALIINEHARMKDALDYLKDFFRNIRAAGFDEIEQDLTQRFEEKLQELESISIDPSNENPKLRDLCFILQEEYHLNPETRTILFVKTRALVDALKKWIKENPKLSFLKPSILTGRGKTNQNIGMTLPAQKCVLDTFRTDKDNKILITTSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTANADLIDKEKMNMYKEEMMNGAILILQTWDEAVFKEKIHQIQIREKIIRDNQGKPEPVPDKKTKKLLCKKCKAFACYTADIRMVEELCVLC

Target ORF information:

RefSeq Version	XM_021064056.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021064056.1

ORF Insert Sequence:

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTGTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACTAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTA 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGAAGCAC CTCATACTTA CAGCCCACTG 
AAGCCAAGAA AGTACCAACT GGAGCTTGCT TTACCTGCTC AGAATGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCTC TTCTTATATG TGAACATCAT 
CTTAAGAAAT TTCCACGAGG ACGAAAGGGG AAGGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAACATTTTG AAAGACTTGG GTACAAAGTT 
GCAGGCATTT CTGGAGCAAC GTCTGACACT GTCTGTGTGG AACAGATTGT TGAGAACAAC 
GACATCATCA TCTTAACACC ACAGATTCTT GTGAACTGTC TTACCAATGG GACAATTCCA 
TCACTCTCTG TGTTTACTTT GATGATATTT GATGAATGCC ACAACACCAG CAAACAGCAT 
CCTTATAATG TCATCATGTT TAATTATCTA GATCGGAAAC TTGGAGGATC TTCAGACTCA 
CTGCCCCAGG TCATTGGACT GACTGCCTCG GTTGGCGTTG GGGATGCAAA AAACAAGGCT 
GAAGCCACAG AATACATCTG CAAATTGTGT GCTTCTCTTG ACACTTCGGT CATAGCAACA 
GTCAGAGACA ACTTGGAAGA ACTAGAGGAA GTTGTGTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT TACGGACCAC TGACAGATTT AAGTGCATCA TATCACAGCT GATGATGGAG 
ATAGAGAGTC TGGCAAAGAG CATCTTTGAA GAACTTGGTA CCATAACTCT TGGAGGCTTA 
TTTCAAATTC AAAATAGTAA TTTCGGAACA CAGAAATATG AGCAGTGGAT TGTCAAGGTT 
CAGAAAGAAT GTGCGGTGTT TCAGATGCCA GACAAAGACA AAGAAAGCAG GATTTGTAAA 
GCACTGTTTT CGTACATGTC ACATTTGCGG ATATACAACG ATGCCCTTAT TATCAATGAA 
CATGCCCGAA TGAAAGATGC TTTGGATTAC TTGAAAGACT TCTTCAGAAA TATCCGAGCA 
GCAGGCTTTG ATGAGATTGA GCAAGATCTC ACTCAGAGAT TTGAAGAAAA GCTGCAAGAA 
CTAGAAAGCA TTTCCATAGA TCCCAGCAAC GAGAACCCTA AACTCAGAGA CCTCTGCTTC 
ATCTTGCAAG AAGAGTACCA CTTAAACCCA GAGACCAGAA CCATTCTCTT TGTGAAAACC 
AGAGCCCTTG TGGATGCTTT AAAGAAATGG ATTAAAGAAA ATCCTAAGCT CAGCTTTCTG 
AAACCTAGCA TATTGACTGG ACGTGGCAAA ACGAATCAGA ACATAGGGAT GACCCTCCCA 
GCCCAGAAGT GTGTACTGGA TACCTTCAGA ACTGACAAGG ATAACAAGAT TCTGATCACC 
ACCTCGGTGG CAGATGAAGG CATCGACATT GCTCAGTGCA ATCTGGTCAT CCTATATGAG 
TATGTGGGCA ACGTCATCAA AATGATCCAA ACCAGAGGCA GAGGAAGAGC AAGAGGGAGC 
AAGTGTTTCC TTCTGACTGC TAACGCTGAT CTGATCGACA AAGAGAAAAT GAACATGTAC 
AAAGAAGAAA TGATGAATGG GGCCATTTTA ATCCTGCAGA CATGGGATGA AGCAGTATTT 
AAAGAAAAGA TTCACCAGAT ACAGATTCGT GAAAAAATCA TCAGGGATAA TCAAGGAAAA 
CCAGAACCTG TGCCTGATAA GAAAACTAAA AAACTACTCT GCAAAAAGTG CAAAGCCTTT 
GCATGTTATA CAGCTGATAT AAGAATGGTG GAGGAACTCT GTGTATTGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX58 ( XM_021064055.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020919714.1 Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe231727
Clone ID Related Accession (Same CDS sequence)	XM_021064056.1 , XM_021064055.1 , XM_021064058.1 , XM_021064057.1
Accession Version	XM_021064055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX58 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010452.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTGTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACTAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTA 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGAAGCAC CTCATACTTA CAGCCCACTG 
AAGCCAAGAA AGTACCAACT GGAGCTTGCT TTACCTGCTC AGAATGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCTC TTCTTATATG TGAACATCAT 
CTTAAGAAAT TTCCACGAGG ACGAAAGGGG AAGGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAACATTTTG AAAGACTTGG GTACAAAGTT 
GCAGGCATTT CTGGAGCAAC GTCTGACACT GTCTGTGTGG AACAGATTGT TGAGAACAAC 
GACATCATCA TCTTAACACC ACAGATTCTT GTGAACTGTC TTACCAATGG GACAATTCCA 
TCACTCTCTG TGTTTACTTT GATGATATTT GATGAATGCC ACAACACCAG CAAACAGCAT 
CCTTATAATG TCATCATGTT TAATTATCTA GATCGGAAAC TTGGAGGATC TTCAGACTCA 
CTGCCCCAGG TCATTGGACT GACTGCCTCG GTTGGCGTTG GGGATGCAAA AAACAAGGCT 
GAAGCCACAG AATACATCTG CAAATTGTGT GCTTCTCTTG ACACTTCGGT CATAGCAACA 
GTCAGAGACA ACTTGGAAGA ACTAGAGGAA GTTGTGTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT TACGGACCAC TGACAGATTT AAGTGCATCA TATCACAGCT GATGATGGAG 
ATAGAGAGTC TGGCAAAGAG CATCTTTGAA GAACTTGGTA CCATAACTCT TGGAGGCTTA 
TTTCAAATTC AAAATAGTAA TTTCGGAACA CAGAAATATG AGCAGTGGAT TGTCAAGGTT 
CAGAAAGAAT GTGCGGTGTT TCAGATGCCA GACAAAGACA AAGAAAGCAG GATTTGTAAA 
GCACTGTTTT CGTACATGTC ACATTTGCGG ATATACAACG ATGCCCTTAT TATCAATGAA 
CATGCCCGAA TGAAAGATGC TTTGGATTAC TTGAAAGACT TCTTCAGAAA TATCCGAGCA 
GCAGGCTTTG ATGAGATTGA GCAAGATCTC ACTCAGAGAT TTGAAGAAAA GCTGCAAGAA 
CTAGAAAGCA TTTCCATAGA TCCCAGCAAC GAGAACCCTA AACTCAGAGA CCTCTGCTTC 
ATCTTGCAAG AAGAGTACCA CTTAAACCCA GAGACCAGAA CCATTCTCTT TGTGAAAACC 
AGAGCCCTTG TGGATGCTTT AAAGAAATGG ATTAAAGAAA ATCCTAAGCT CAGCTTTCTG 
AAACCTAGCA TATTGACTGG ACGTGGCAAA ACGAATCAGA ACATAGGGAT GACCCTCCCA 
GCCCAGAAGT GTGTACTGGA TACCTTCAGA ACTGACAAGG ATAACAAGAT TCTGATCACC 
ACCTCGGTGG CAGATGAAGG CATCGACATT GCTCAGTGCA ATCTGGTCAT CCTATATGAG 
TATGTGGGCA ACGTCATCAA AATGATCCAA ACCAGAGGCA GAGGAAGAGC AAGAGGGAGC 
AAGTGTTTCC TTCTGACTGC TAACGCTGAT CTGATCGACA AAGAGAAAAT GAACATGTAC 
AAAGAAGAAA TGATGAATGG GGCCATTTTA ATCCTGCAGA CATGGGATGA AGCAGTATTT 
AAAGAAAAGA TTCACCAGAT ACAGATTCGT GAAAAAATCA TCAGGGATAA TCAAGGAAAA 
CCAGAACCTG TGCCTGATAA GAAAACTAAA AAACTACTCT GCAAAAAGTG CAAAGCCTTT 
GCATGTTATA CAGCTGATAT AAGAATGGTG GAGGAACTCT GTGTATTGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020919714.1
CDS	82..2595
Translation	MTAEQRRNLHAFGDYVRKTLDPTFILSYMAPWFRDDEVQHIQAEKNNKGPTEAASLFLQFLLELQEEGWFRGFLDALNQAGYCGLCEAIESWDFQKIEKLEEYRSLLRRLQPEFKTTINPKDILPEIAECLISQECEEILQICSSKGLMAGAEKMVECLLRSDKENWPKTLKLALEKEESRFSELWMVDKGAEDVKMKDLEDDEMKTCDVQIFYKEEPENQNLSQNSCSSSEAPHTYSPLKPRKYQLELALPAQNGKNTIICAPTGCGKTFVSLLICEHHLKKFPRGRKGKVVFFAIQLPVYEQQKSVFSKHFERLGYKVAGISGATSDTVCVEQIVENNDIIILTPQILVNCLTNGTIPSLSVFTLMIFDECHNTSKQHPYNVIMFNYLDRKLGGSSDSLPQVIGLTASVGVGDAKNKAEATEYICKLCASLDTSVIATVRDNLEELEEVVYKPQKFFRKVELRTTDRFKCIISQLMMEIESLAKSIFEELGTITLGGLFQIQNSNFGTQKYEQWIVKVQKECAVFQMPDKDKESRICKALFSYMSHLRIYNDALIINEHARMKDALDYLKDFFRNIRAAGFDEIEQDLTQRFEEKLQELESISIDPSNENPKLRDLCFILQEEYHLNPETRTILFVKTRALVDALKKWIKENPKLSFLKPSILTGRGKTNQNIGMTLPAQKCVLDTFRTDKDNKILITTSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTANADLIDKEKMNMYKEEMMNGAILILQTWDEAVFKEKIHQIQIREKIIRDNQGKPEPVPDKKTKKLLCKKCKAFACYTADIRMVEELCVLC

Target ORF information:

RefSeq Version	XM_021064055.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021064055.1

ORF Insert Sequence:

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTGTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACTAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTA 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGAAGCAC CTCATACTTA CAGCCCACTG 
AAGCCAAGAA AGTACCAACT GGAGCTTGCT TTACCTGCTC AGAATGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCTC TTCTTATATG TGAACATCAT 
CTTAAGAAAT TTCCACGAGG ACGAAAGGGG AAGGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAACATTTTG AAAGACTTGG GTACAAAGTT 
GCAGGCATTT CTGGAGCAAC GTCTGACACT GTCTGTGTGG AACAGATTGT TGAGAACAAC 
GACATCATCA TCTTAACACC ACAGATTCTT GTGAACTGTC TTACCAATGG GACAATTCCA 
TCACTCTCTG TGTTTACTTT GATGATATTT GATGAATGCC ACAACACCAG CAAACAGCAT 
CCTTATAATG TCATCATGTT TAATTATCTA GATCGGAAAC TTGGAGGATC TTCAGACTCA 
CTGCCCCAGG TCATTGGACT GACTGCCTCG GTTGGCGTTG GGGATGCAAA AAACAAGGCT 
GAAGCCACAG AATACATCTG CAAATTGTGT GCTTCTCTTG ACACTTCGGT CATAGCAACA 
GTCAGAGACA ACTTGGAAGA ACTAGAGGAA GTTGTGTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT TACGGACCAC TGACAGATTT AAGTGCATCA TATCACAGCT GATGATGGAG 
ATAGAGAGTC TGGCAAAGAG CATCTTTGAA GAACTTGGTA CCATAACTCT TGGAGGCTTA 
TTTCAAATTC AAAATAGTAA TTTCGGAACA CAGAAATATG AGCAGTGGAT TGTCAAGGTT 
CAGAAAGAAT GTGCGGTGTT TCAGATGCCA GACAAAGACA AAGAAAGCAG GATTTGTAAA 
GCACTGTTTT CGTACATGTC ACATTTGCGG ATATACAACG ATGCCCTTAT TATCAATGAA 
CATGCCCGAA TGAAAGATGC TTTGGATTAC TTGAAAGACT TCTTCAGAAA TATCCGAGCA 
GCAGGCTTTG ATGAGATTGA GCAAGATCTC ACTCAGAGAT TTGAAGAAAA GCTGCAAGAA 
CTAGAAAGCA TTTCCATAGA TCCCAGCAAC GAGAACCCTA AACTCAGAGA CCTCTGCTTC 
ATCTTGCAAG AAGAGTACCA CTTAAACCCA GAGACCAGAA CCATTCTCTT TGTGAAAACC 
AGAGCCCTTG TGGATGCTTT AAAGAAATGG ATTAAAGAAA ATCCTAAGCT CAGCTTTCTG 
AAACCTAGCA TATTGACTGG ACGTGGCAAA ACGAATCAGA ACATAGGGAT GACCCTCCCA 
GCCCAGAAGT GTGTACTGGA TACCTTCAGA ACTGACAAGG ATAACAAGAT TCTGATCACC 
ACCTCGGTGG CAGATGAAGG CATCGACATT GCTCAGTGCA ATCTGGTCAT CCTATATGAG 
TATGTGGGCA ACGTCATCAA AATGATCCAA ACCAGAGGCA GAGGAAGAGC AAGAGGGAGC 
AAGTGTTTCC TTCTGACTGC TAACGCTGAT CTGATCGACA AAGAGAAAAT GAACATGTAC 
AAAGAAGAAA TGATGAATGG GGCCATTTTA ATCCTGCAGA CATGGGATGA AGCAGTATTT 
AAAGAAAAGA TTCACCAGAT ACAGATTCGT GAAAAAATCA TCAGGGATAA TCAAGGAAAA 
CCAGAACCTG TGCCTGATAA GAAAACTAAA AAACTACTCT GCAAAAAGTG CAAAGCCTTT 
GCATGTTATA CAGCTGATAT AAGAATGGTG GAGGAACTCT GTGTATTGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX58 ( XM_021064058.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020919717.1 Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OSe231727
Clone ID Related Accession (Same CDS sequence)	XM_021064056.1 , XM_021064055.1 , XM_021064058.1 , XM_021064057.1
Accession Version	XM_021064058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX58 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010452.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTGTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACTAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTA 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGAAGCAC CTCATACTTA CAGCCCACTG 
AAGCCAAGAA AGTACCAACT GGAGCTTGCT TTACCTGCTC AGAATGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCTC TTCTTATATG TGAACATCAT 
CTTAAGAAAT TTCCACGAGG ACGAAAGGGG AAGGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAACATTTTG AAAGACTTGG GTACAAAGTT 
GCAGGCATTT CTGGAGCAAC GTCTGACACT GTCTGTGTGG AACAGATTGT TGAGAACAAC 
GACATCATCA TCTTAACACC ACAGATTCTT GTGAACTGTC TTACCAATGG GACAATTCCA 
TCACTCTCTG TGTTTACTTT GATGATATTT GATGAATGCC ACAACACCAG CAAACAGCAT 
CCTTATAATG TCATCATGTT TAATTATCTA GATCGGAAAC TTGGAGGATC TTCAGACTCA 
CTGCCCCAGG TCATTGGACT GACTGCCTCG GTTGGCGTTG GGGATGCAAA AAACAAGGCT 
GAAGCCACAG AATACATCTG CAAATTGTGT GCTTCTCTTG ACACTTCGGT CATAGCAACA 
GTCAGAGACA ACTTGGAAGA ACTAGAGGAA GTTGTGTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT TACGGACCAC TGACAGATTT AAGTGCATCA TATCACAGCT GATGATGGAG 
ATAGAGAGTC TGGCAAAGAG CATCTTTGAA GAACTTGGTA CCATAACTCT TGGAGGCTTA 
TTTCAAATTC AAAATAGTAA TTTCGGAACA CAGAAATATG AGCAGTGGAT TGTCAAGGTT 
CAGAAAGAAT GTGCGGTGTT TCAGATGCCA GACAAAGACA AAGAAAGCAG GATTTGTAAA 
GCACTGTTTT CGTACATGTC ACATTTGCGG ATATACAACG ATGCCCTTAT TATCAATGAA 
CATGCCCGAA TGAAAGATGC TTTGGATTAC TTGAAAGACT TCTTCAGAAA TATCCGAGCA 
GCAGGCTTTG ATGAGATTGA GCAAGATCTC ACTCAGAGAT TTGAAGAAAA GCTGCAAGAA 
CTAGAAAGCA TTTCCATAGA TCCCAGCAAC GAGAACCCTA AACTCAGAGA CCTCTGCTTC 
ATCTTGCAAG AAGAGTACCA CTTAAACCCA GAGACCAGAA CCATTCTCTT TGTGAAAACC 
AGAGCCCTTG TGGATGCTTT AAAGAAATGG ATTAAAGAAA ATCCTAAGCT CAGCTTTCTG 
AAACCTAGCA TATTGACTGG ACGTGGCAAA ACGAATCAGA ACATAGGGAT GACCCTCCCA 
GCCCAGAAGT GTGTACTGGA TACCTTCAGA ACTGACAAGG ATAACAAGAT TCTGATCACC 
ACCTCGGTGG CAGATGAAGG CATCGACATT GCTCAGTGCA ATCTGGTCAT CCTATATGAG 
TATGTGGGCA ACGTCATCAA AATGATCCAA ACCAGAGGCA GAGGAAGAGC AAGAGGGAGC 
AAGTGTTTCC TTCTGACTGC TAACGCTGAT CTGATCGACA AAGAGAAAAT GAACATGTAC 
AAAGAAGAAA TGATGAATGG GGCCATTTTA ATCCTGCAGA CATGGGATGA AGCAGTATTT 
AAAGAAAAGA TTCACCAGAT ACAGATTCGT GAAAAAATCA TCAGGGATAA TCAAGGAAAA 
CCAGAACCTG TGCCTGATAA GAAAACTAAA AAACTACTCT GCAAAAAGTG CAAAGCCTTT 
GCATGTTATA CAGCTGATAT AAGAATGGTG GAGGAACTCT GTGTATTGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020919717.1
CDS	92..2605
Translation	MTAEQRRNLHAFGDYVRKTLDPTFILSYMAPWFRDDEVQHIQAEKNNKGPTEAASLFLQFLLELQEEGWFRGFLDALNQAGYCGLCEAIESWDFQKIEKLEEYRSLLRRLQPEFKTTINPKDILPEIAECLISQECEEILQICSSKGLMAGAEKMVECLLRSDKENWPKTLKLALEKEESRFSELWMVDKGAEDVKMKDLEDDEMKTCDVQIFYKEEPENQNLSQNSCSSSEAPHTYSPLKPRKYQLELALPAQNGKNTIICAPTGCGKTFVSLLICEHHLKKFPRGRKGKVVFFAIQLPVYEQQKSVFSKHFERLGYKVAGISGATSDTVCVEQIVENNDIIILTPQILVNCLTNGTIPSLSVFTLMIFDECHNTSKQHPYNVIMFNYLDRKLGGSSDSLPQVIGLTASVGVGDAKNKAEATEYICKLCASLDTSVIATVRDNLEELEEVVYKPQKFFRKVELRTTDRFKCIISQLMMEIESLAKSIFEELGTITLGGLFQIQNSNFGTQKYEQWIVKVQKECAVFQMPDKDKESRICKALFSYMSHLRIYNDALIINEHARMKDALDYLKDFFRNIRAAGFDEIEQDLTQRFEEKLQELESISIDPSNENPKLRDLCFILQEEYHLNPETRTILFVKTRALVDALKKWIKENPKLSFLKPSILTGRGKTNQNIGMTLPAQKCVLDTFRTDKDNKILITTSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTANADLIDKEKMNMYKEEMMNGAILILQTWDEAVFKEKIHQIQIREKIIRDNQGKPEPVPDKKTKKLLCKKCKAFACYTADIRMVEELCVLC

Target ORF information:

RefSeq Version	XM_021064058.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021064058.1

ORF Insert Sequence:

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTGTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACTAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTA 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGAAGCAC CTCATACTTA CAGCCCACTG 
AAGCCAAGAA AGTACCAACT GGAGCTTGCT TTACCTGCTC AGAATGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCTC TTCTTATATG TGAACATCAT 
CTTAAGAAAT TTCCACGAGG ACGAAAGGGG AAGGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAACATTTTG AAAGACTTGG GTACAAAGTT 
GCAGGCATTT CTGGAGCAAC GTCTGACACT GTCTGTGTGG AACAGATTGT TGAGAACAAC 
GACATCATCA TCTTAACACC ACAGATTCTT GTGAACTGTC TTACCAATGG GACAATTCCA 
TCACTCTCTG TGTTTACTTT GATGATATTT GATGAATGCC ACAACACCAG CAAACAGCAT 
CCTTATAATG TCATCATGTT TAATTATCTA GATCGGAAAC TTGGAGGATC TTCAGACTCA 
CTGCCCCAGG TCATTGGACT GACTGCCTCG GTTGGCGTTG GGGATGCAAA AAACAAGGCT 
GAAGCCACAG AATACATCTG CAAATTGTGT GCTTCTCTTG ACACTTCGGT CATAGCAACA 
GTCAGAGACA ACTTGGAAGA ACTAGAGGAA GTTGTGTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT TACGGACCAC TGACAGATTT AAGTGCATCA TATCACAGCT GATGATGGAG 
ATAGAGAGTC TGGCAAAGAG CATCTTTGAA GAACTTGGTA CCATAACTCT TGGAGGCTTA 
TTTCAAATTC AAAATAGTAA TTTCGGAACA CAGAAATATG AGCAGTGGAT TGTCAAGGTT 
CAGAAAGAAT GTGCGGTGTT TCAGATGCCA GACAAAGACA AAGAAAGCAG GATTTGTAAA 
GCACTGTTTT CGTACATGTC ACATTTGCGG ATATACAACG ATGCCCTTAT TATCAATGAA 
CATGCCCGAA TGAAAGATGC TTTGGATTAC TTGAAAGACT TCTTCAGAAA TATCCGAGCA 
GCAGGCTTTG ATGAGATTGA GCAAGATCTC ACTCAGAGAT TTGAAGAAAA GCTGCAAGAA 
CTAGAAAGCA TTTCCATAGA TCCCAGCAAC GAGAACCCTA AACTCAGAGA CCTCTGCTTC 
ATCTTGCAAG AAGAGTACCA CTTAAACCCA GAGACCAGAA CCATTCTCTT TGTGAAAACC 
AGAGCCCTTG TGGATGCTTT AAAGAAATGG ATTAAAGAAA ATCCTAAGCT CAGCTTTCTG 
AAACCTAGCA TATTGACTGG ACGTGGCAAA ACGAATCAGA ACATAGGGAT GACCCTCCCA 
GCCCAGAAGT GTGTACTGGA TACCTTCAGA ACTGACAAGG ATAACAAGAT TCTGATCACC 
ACCTCGGTGG CAGATGAAGG CATCGACATT GCTCAGTGCA ATCTGGTCAT CCTATATGAG 
TATGTGGGCA ACGTCATCAA AATGATCCAA ACCAGAGGCA GAGGAAGAGC AAGAGGGAGC 
AAGTGTTTCC TTCTGACTGC TAACGCTGAT CTGATCGACA AAGAGAAAAT GAACATGTAC 
AAAGAAGAAA TGATGAATGG GGCCATTTTA ATCCTGCAGA CATGGGATGA AGCAGTATTT 
AAAGAAAAGA TTCACCAGAT ACAGATTCGT GAAAAAATCA TCAGGGATAA TCAAGGAAAA 
CCAGAACCTG TGCCTGATAA GAAAACTAAA AAACTACTCT GCAAAAAGTG CAAAGCCTTT 
GCATGTTATA CAGCTGATAT AAGAATGGTG GAGGAACTCT GTGTATTGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX58 ( XM_021064057.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020919716.1 Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSe231727
Clone ID Related Accession (Same CDS sequence)	XM_021064056.1 , XM_021064055.1 , XM_021064058.1 , XM_021064057.1
Accession Version	XM_021064057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	probable ATP-dependent RNA helicase DDX58 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010452.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTGTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACTAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTA 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGAAGCAC CTCATACTTA CAGCCCACTG 
AAGCCAAGAA AGTACCAACT GGAGCTTGCT TTACCTGCTC AGAATGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCTC TTCTTATATG TGAACATCAT 
CTTAAGAAAT TTCCACGAGG ACGAAAGGGG AAGGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAACATTTTG AAAGACTTGG GTACAAAGTT 
GCAGGCATTT CTGGAGCAAC GTCTGACACT GTCTGTGTGG AACAGATTGT TGAGAACAAC 
GACATCATCA TCTTAACACC ACAGATTCTT GTGAACTGTC TTACCAATGG GACAATTCCA 
TCACTCTCTG TGTTTACTTT GATGATATTT GATGAATGCC ACAACACCAG CAAACAGCAT 
CCTTATAATG TCATCATGTT TAATTATCTA GATCGGAAAC TTGGAGGATC TTCAGACTCA 
CTGCCCCAGG TCATTGGACT GACTGCCTCG GTTGGCGTTG GGGATGCAAA AAACAAGGCT 
GAAGCCACAG AATACATCTG CAAATTGTGT GCTTCTCTTG ACACTTCGGT CATAGCAACA 
GTCAGAGACA ACTTGGAAGA ACTAGAGGAA GTTGTGTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT TACGGACCAC TGACAGATTT AAGTGCATCA TATCACAGCT GATGATGGAG 
ATAGAGAGTC TGGCAAAGAG CATCTTTGAA GAACTTGGTA CCATAACTCT TGGAGGCTTA 
TTTCAAATTC AAAATAGTAA TTTCGGAACA CAGAAATATG AGCAGTGGAT TGTCAAGGTT 
CAGAAAGAAT GTGCGGTGTT TCAGATGCCA GACAAAGACA AAGAAAGCAG GATTTGTAAA 
GCACTGTTTT CGTACATGTC ACATTTGCGG ATATACAACG ATGCCCTTAT TATCAATGAA 
CATGCCCGAA TGAAAGATGC TTTGGATTAC TTGAAAGACT TCTTCAGAAA TATCCGAGCA 
GCAGGCTTTG ATGAGATTGA GCAAGATCTC ACTCAGAGAT TTGAAGAAAA GCTGCAAGAA 
CTAGAAAGCA TTTCCATAGA TCCCAGCAAC GAGAACCCTA AACTCAGAGA CCTCTGCTTC 
ATCTTGCAAG AAGAGTACCA CTTAAACCCA GAGACCAGAA CCATTCTCTT TGTGAAAACC 
AGAGCCCTTG TGGATGCTTT AAAGAAATGG ATTAAAGAAA ATCCTAAGCT CAGCTTTCTG 
AAACCTAGCA TATTGACTGG ACGTGGCAAA ACGAATCAGA ACATAGGGAT GACCCTCCCA 
GCCCAGAAGT GTGTACTGGA TACCTTCAGA ACTGACAAGG ATAACAAGAT TCTGATCACC 
ACCTCGGTGG CAGATGAAGG CATCGACATT GCTCAGTGCA ATCTGGTCAT CCTATATGAG 
TATGTGGGCA ACGTCATCAA AATGATCCAA ACCAGAGGCA GAGGAAGAGC AAGAGGGAGC 
AAGTGTTTCC TTCTGACTGC TAACGCTGAT CTGATCGACA AAGAGAAAAT GAACATGTAC 
AAAGAAGAAA TGATGAATGG GGCCATTTTA ATCCTGCAGA CATGGGATGA AGCAGTATTT 
AAAGAAAAGA TTCACCAGAT ACAGATTCGT GAAAAAATCA TCAGGGATAA TCAAGGAAAA 
CCAGAACCTG TGCCTGATAA GAAAACTAAA AAACTACTCT GCAAAAAGTG CAAAGCCTTT 
GCATGTTATA CAGCTGATAT AAGAATGGTG GAGGAACTCT GTGTATTGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020919716.1
CDS	82..2595
Translation	MTAEQRRNLHAFGDYVRKTLDPTFILSYMAPWFRDDEVQHIQAEKNNKGPTEAASLFLQFLLELQEEGWFRGFLDALNQAGYCGLCEAIESWDFQKIEKLEEYRSLLRRLQPEFKTTINPKDILPEIAECLISQECEEILQICSSKGLMAGAEKMVECLLRSDKENWPKTLKLALEKEESRFSELWMVDKGAEDVKMKDLEDDEMKTCDVQIFYKEEPENQNLSQNSCSSSEAPHTYSPLKPRKYQLELALPAQNGKNTIICAPTGCGKTFVSLLICEHHLKKFPRGRKGKVVFFAIQLPVYEQQKSVFSKHFERLGYKVAGISGATSDTVCVEQIVENNDIIILTPQILVNCLTNGTIPSLSVFTLMIFDECHNTSKQHPYNVIMFNYLDRKLGGSSDSLPQVIGLTASVGVGDAKNKAEATEYICKLCASLDTSVIATVRDNLEELEEVVYKPQKFFRKVELRTTDRFKCIISQLMMEIESLAKSIFEELGTITLGGLFQIQNSNFGTQKYEQWIVKVQKECAVFQMPDKDKESRICKALFSYMSHLRIYNDALIINEHARMKDALDYLKDFFRNIRAAGFDEIEQDLTQRFEEKLQELESISIDPSNENPKLRDLCFILQEEYHLNPETRTILFVKTRALVDALKKWIKENPKLSFLKPSILTGRGKTNQNIGMTLPAQKCVLDTFRTDKDNKILITTSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTANADLIDKEKMNMYKEEMMNGAILILQTWDEAVFKEKIHQIQIREKIIRDNQGKPEPVPDKKTKKLLCKKCKAFACYTADIRMVEELCVLC

Target ORF information:

RefSeq Version	XM_021064057.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa DExD/H-box helicase 58 (DDX58), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021064057.1

ORF Insert Sequence:

ATGACAGCAG AGCAGCGGCG GAATCTGCAC GCTTTCGGGG ACTATGTCAG GAAGACTCTC 
GACCCCACCT TCATCCTGAG CTACATGGCC CCCTGGTTTA GGGACGATGA GGTGCAGCAT 
ATTCAGGCTG AGAAAAACAA CAAGGGCCCG ACAGAGGCTG CATCACTTTT TCTCCAGTTC 
CTGTTGGAGC TCCAGGAGGA AGGCTGGTTC CGAGGCTTTT TGGATGCCCT TAACCAAGCA 
GGTTATTGTG GACTTTGTGA AGCCATTGAA AGTTGGGATT TCCAAAAAAT TGAGAAGTTG 
GAGGAGTACA GATCACTCTT AAGGCGTTTA CAACCAGAAT TTAAAACTAC GATCAATCCA 
AAAGATATCC TTCCTGAAAT AGCTGAATGT TTAATTAGTC AGGAATGTGA AGAAATTCTA 
CAGATTTGTT CCAGCAAGGG GCTGATGGCA GGTGCAGAGA AAATGGTTGA ATGCCTCCTC 
AGATCAGACA AGGAGAATTG GCCTAAAACT TTGAAACTTG CTTTGGAGAA AGAAGAGAGC 
AGGTTCAGTG AACTGTGGAT GGTAGACAAA GGTGCAGAAG ATGTTAAAAT GAAAGATCTT 
GAGGATGATG AAATGAAAAC TTGTGATGTA CAGATTTTCT ACAAGGAAGA ACCAGAAAAC 
CAGAATCTTA GTCAGAATTC GTGTTCCTCT TCAGAAGCAC CTCATACTTA CAGCCCACTG 
AAGCCAAGAA AGTACCAACT GGAGCTTGCT TTACCTGCTC AGAATGGAAA AAACACAATA 
ATATGTGCTC CTACTGGTTG TGGAAAAACC TTTGTTTCTC TTCTTATATG TGAACATCAT 
CTTAAGAAAT TTCCACGAGG ACGAAAGGGG AAGGTTGTCT TTTTTGCTAT TCAACTCCCA 
GTGTATGAGC AGCAGAAATC TGTGTTCTCA AAACATTTTG AAAGACTTGG GTACAAAGTT 
GCAGGCATTT CTGGAGCAAC GTCTGACACT GTCTGTGTGG AACAGATTGT TGAGAACAAC 
GACATCATCA TCTTAACACC ACAGATTCTT GTGAACTGTC TTACCAATGG GACAATTCCA 
TCACTCTCTG TGTTTACTTT GATGATATTT GATGAATGCC ACAACACCAG CAAACAGCAT 
CCTTATAATG TCATCATGTT TAATTATCTA GATCGGAAAC TTGGAGGATC TTCAGACTCA 
CTGCCCCAGG TCATTGGACT GACTGCCTCG GTTGGCGTTG GGGATGCAAA AAACAAGGCT 
GAAGCCACAG AATACATCTG CAAATTGTGT GCTTCTCTTG ACACTTCGGT CATAGCAACA 
GTCAGAGACA ACTTGGAAGA ACTAGAGGAA GTTGTGTATA AGCCCCAGAA GTTTTTCAGG 
AAAGTGGAAT TACGGACCAC TGACAGATTT AAGTGCATCA TATCACAGCT GATGATGGAG 
ATAGAGAGTC TGGCAAAGAG CATCTTTGAA GAACTTGGTA CCATAACTCT TGGAGGCTTA 
TTTCAAATTC AAAATAGTAA TTTCGGAACA CAGAAATATG AGCAGTGGAT TGTCAAGGTT 
CAGAAAGAAT GTGCGGTGTT TCAGATGCCA GACAAAGACA AAGAAAGCAG GATTTGTAAA 
GCACTGTTTT CGTACATGTC ACATTTGCGG ATATACAACG ATGCCCTTAT TATCAATGAA 
CATGCCCGAA TGAAAGATGC TTTGGATTAC TTGAAAGACT TCTTCAGAAA TATCCGAGCA 
GCAGGCTTTG ATGAGATTGA GCAAGATCTC ACTCAGAGAT TTGAAGAAAA GCTGCAAGAA 
CTAGAAAGCA TTTCCATAGA TCCCAGCAAC GAGAACCCTA AACTCAGAGA CCTCTGCTTC 
ATCTTGCAAG AAGAGTACCA CTTAAACCCA GAGACCAGAA CCATTCTCTT TGTGAAAACC 
AGAGCCCTTG TGGATGCTTT AAAGAAATGG ATTAAAGAAA ATCCTAAGCT CAGCTTTCTG 
AAACCTAGCA TATTGACTGG ACGTGGCAAA ACGAATCAGA ACATAGGGAT GACCCTCCCA 
GCCCAGAAGT GTGTACTGGA TACCTTCAGA ACTGACAAGG ATAACAAGAT TCTGATCACC 
ACCTCGGTGG CAGATGAAGG CATCGACATT GCTCAGTGCA ATCTGGTCAT CCTATATGAG 
TATGTGGGCA ACGTCATCAA AATGATCCAA ACCAGAGGCA GAGGAAGAGC AAGAGGGAGC 
AAGTGTTTCC TTCTGACTGC TAACGCTGAT CTGATCGACA AAGAGAAAAT GAACATGTAC 
AAAGAAGAAA TGATGAATGG GGCCATTTTA ATCCTGCAGA CATGGGATGA AGCAGTATTT 
AAAGAAAAGA TTCACCAGAT ACAGATTCGT GAAAAAATCA TCAGGGATAA TCAAGGAAAA 
CCAGAACCTG TGCCTGATAA GAAAACTAAA AAACTACTCT GCAAAAAGTG CAAAGCCTTT 
GCATGTTATA CAGCTGATAT AAGAATGGTG GAGGAACTCT GTGTATTGTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nucleocapsid protein of porcine reproductive and respiratory syndrome virus antagonizes the antiviral activity of TRIM25 by interfering with TRIM25-mediated RIG-I ubiquitination.
Veterinary microbiology233140-146(2019 Jun)
Zhao K,Li LW,Jiang YF,Gao F,Zhang YJ,Zhao WY,Li GX,Yu LX,Zhou YJ,Tong GZ

RIG-I is responsible for activation of type I interferon pathway in Seneca Valley virus-infected porcine cells to suppress viral replication.
Virology journal15(1)162(2018 10)
Li P,Zhang X,Cao W,Yang F,Du X,Shi Z,Zhang M,Liu X,Zhu Z,Zheng H

Porcine circovirus 2 infection induces IFNβ expression through increased expression of genes involved in RIG-I and IRF7 signaling pathways.
Virus research25338-47(2018 07)
Dvorak CMT,Puvanendiran S,Murtaugh MP

Identification of the RNA Pseudoknot within the 3' End of the Porcine Reproductive and Respiratory Syndrome Virus Genome as a Pathogen-Associated Molecular Pattern To Activate Antiviral Signaling via RIG-I and Toll-Like Receptor 3.
Journal of virology92(12)(2018 06)
Xie S,Chen XX,Qiao S,Li R,Sun Y,Xia S,Wang LJ,Luo X,Deng R,Zhou EM,Zhang GP

Interactions between the influenza A virus RNA polymerase components and retinoic acid-inducible gene I.
Journal of virology88(18)10432-47(2014 Sep)
Li W,Chen H,Sutton T,Obadan A,Perez DR

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DDX58 cDNA ORF clone, Sus scrofa(pig)

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