AFDN gene cDNA ORF clone, Sus scrofa(pig)

mRNA	Protein	Name
XM_013984265.1	XP_013839719.1	afadin isoform X1
XM_013984289.1	XP_013839743.1	afadin isoform X8
XM_013984289.2	XP_013839743.1	afadin isoform X9
XM_013984302.1	XP_013839756.1	afadin isoform X12
XM_013984278.2	XP_013839732.1	afadin isoform X6
XM_021085803.1	XP_020941462.1	afadin isoform X8
XM_021085815.1	XP_020941474.1	afadin isoform X10
XM_013984272.1	XP_013839726.1	afadin isoform X3
XM_013984292.1	XP_013839746.1	afadin isoform X9
XM_013984285.1	XP_013839739.1	afadin isoform X7
XM_013984285.2	XP_013839739.1	afadin isoform X7
XM_013984296.2	XP_013839750.1	afadin isoform X11
XM_013984300.1	XP_013839754.1	afadin isoform X11
XM_013984306.1	XP_013839760.1	afadin isoform X14
XM_021085787.1	XP_020941446.1	afadin isoform X5
XM_021085843.1	XP_020941502.1	afadin isoform X17
XM_013984277.1	XP_013839731.1	afadin isoform X4
XM_013984277.2	XP_013839731.1	afadin isoform X3
XM_013984304.1	XP_013839758.1	afadin isoform X13
XM_021085831.1	XP_020941490.1	afadin isoform X13
XM_021085833.1	XP_020941492.1	afadin isoform X14
XM_021085776.1	XP_020941435.1	afadin isoform X4
XM_021085824.1	XP_020941483.1	afadin isoform X12
XM_013984272.2	XP_013839726.1	afadin isoform X2
XM_013984296.1	XP_013839750.1	afadin isoform X10
XM_013984302.2	XP_013839756.1	afadin isoform X15
XM_013984267.1	XP_013839721.1	afadin isoform X2
XM_013984278.1	XP_013839732.1	afadin isoform X5
XM_013984282.1	XP_013839736.1	afadin isoform X6
XM_013984267.2	XP_013839721.1	afadin isoform X1
XM_021085839.1	XP_020941498.1	afadin isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe229404	XM_013984267.2 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229405	XM_013984272.2 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229406	XM_013984277.2 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229407	XM_021085776.1 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229408	XM_021085787.1 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229409	XM_013984278.2 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229410	XM_013984285.2 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229411	XM_021085803.1 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229412	XM_013984289.2 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229413	XM_021085815.1 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229414	XM_013984296.2 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229415	XM_021085824.1 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229416	XM_021085831.1 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229417	XM_021085833.1 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229418	XM_013984302.2 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229419	XM_021085839.1 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe229420	XM_021085843.1 Latest version!	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSe30605	XM_013984265.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30606	XM_013984267.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30607	XM_013984272.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30608	XM_013984277.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30609	XM_013984278.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30610	XM_013984282.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30611	XM_013984285.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30612	XM_013984289.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30613	XM_013984292.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30614	XM_013984296.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30615	XM_013984300.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30616	XM_013984302.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30617	XM_013984304.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe30618	XM_013984306.1 Latest version!	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

AFDN ( XM_013984267.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839721.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe229404
Clone ID Related Accession (Same CDS sequence)	XM_013984267.2
Accession Version	XM_013984267.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013984267.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGACGCGGCC GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCCCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839721.1
CDS	22..5514
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984267.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984267.2

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGACGCGGCC GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCCCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984272.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839726.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

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CloneID	OSe229405
Clone ID Related Accession (Same CDS sequence)	XM_013984272.2
Accession Version	XM_013984272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013984272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GGAAGTAGAC 
CAGGTGGCTG AGAAACAGAA GAACATCGCG GGGGCGCTTG CGTTCTGGAT GGCCAACGCG 
TCGGAACTCC TCAACTTCAT CAAGCAGGAC CGAGACCTCA GTCGAATCAC ACTGGACGCC 
CAGGATGTTT TGGCTCACCT GGTTCAGATG GCCTTTAAAT ACCTGGTTCA CTGTCTTCAG 
TCAGAGCTGA ACAACTACAT GCCGGCCTTT CTGGACGACC CCGAGGAGAA CAGTCTGCAA 
AGACCAAAAA TAGAGGACGT CCTCCACACG CTCACGGGCG CCATGTCGCT GCTGCGGCGC 
TGCCGGGTCA ACGCCGCCCT CACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCATCAAC 
ATGTGGCTGT TCAACAGGCT GCTGACCGAC CCCGAGTCGG GCCTGTGCTC CCACTACTGG 
GGCGCCATCG TCCGCCAGCA GCTGGGCCAC GTGGAGGCCT GGGCGGAGAA GCAGGGCCTG 
GAGCTGGCTG CCGACTGCCA CCTCAGCAGG GTCGTGCAGG CGACCACCCT GCTCACGATG 
GACAAGTACG CCCCGGAGGA CGCGGCCGGC ATCAACAGCA CCTGCTTCAA GCTGAACTCG 
CTGCAGCTGC AGGCCTTGCT GCACAACTAC CACTGTGCGC CCGACGAGCC CCTGGTCCCG 
CCGGATCTGA TAGAAAGCGT GGTGGCCGTG GCTGAAAACA CGGCGGACGA GCTGGCGCGC 
AGCGACGGCA GGGAGGTGCG GCTGGAGGAG GACCCTGACC TGCAGCTGCC CTTCCTGCTG 
CCGGAGGACG GCTACTCGTG TGACGTCGTC AGAAACATCC CCAACGGCTT GCAGGAGTTT 
TTGGATCCTT TGTGCCAGAG AGGGTTTTGC AGGCTGACGC CCCAGCCGCG CTCTCCGGGT 
TCCTGGACGA TACATTTTGA AGGTGCGGAG TACGAGAGCC GCCTCGCACG GGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCC GAAGTCATCA CTGTGACCCT GAAGAAGCAG 
AACGGGATGG GCCTCAGCAT CGTCGCAGCG AAGGGCGCAG GTCAAGACAA ACTCGGGATC 
TACGTCAAGT CGGTGGTGAG AGGAGGAGCT GCAGACGTGG ACGGGCGGCT GGCGGCGGGG 
GACCAGCTCC TCAGTGTGGA CGGACGAAGC CTGGTGGGCC TCTCTCAGGA AAGGGCAGCA 
GAGCTGATGA CAAGGACAAG TTCCGTGGTG ACCCTGGAGG TAGCGAAGCA GGGAGCCATT 
TACCACGGCC TCGCCACCCT GCTCAACCAG CCCTCGCCCA TGATGCAGAG AATTTCAGAC 
CGGCGTGGAT CAGGTAAACC CCGACCAAAG AGCGAAGGTT TTGAGCTCTA TAATAACTCA 
GCTCAGAACG GGTCCCCCGA GAGCCCCCCG CTGCCCTGGG CCGAGTACAG CGAGCCAAAG 
AAGCTGCCTG GCGACGAGAG GCTGACGAAG AACCGCGCCG ACCACCGCTC CAGCCCCAAC 
GTTGCAAATC AGCCTCCTAG TCCTGGTGGG AAAGGTGCAC ATGCCGCTGG AACAGCAGCC 
AAGATCACAT CCGTCTCCAC TGGCAACCTC TGCACCGAGG AGCAGACGCC CCCGCCGGGG 
ACGGAGGCCT ACCCCATCCC CACGCAGGCC TACCCCCGAG AGTACTTCAC CTTCCCGGCC 
TCCAGGTCCC AGGACCGCGT GGTCCCCCCC CAGAGCCAGT GGCCAAGCTA CGAGGAGAAG 
CCGCACGCGG ACAGCAGCCA CTCCAGCCTC GCGGTTCAGC GGGTGACCCG CTCCCAGGAG 
GAGCTGCGGG AGGACGCGGG GGCCTACCCA CTGCACAGGA TGGAGGCGGT GATGGACCGC 
AAGTCTGACA GCGACATGTG GGTCGCCCAG AGCTCCTCGA TGGGCTCCAG CGCGTCCAGC 
CAGGAGCACC TGGACCCCTC TTCCAAGGCG GGCACCCCCG CGTCCACCCT CACCAAGAGC 
GGCCCGGGGC GCTGGAAGAC CCCTGCAGTT CTGCAGCCCG CGCCCGGCCC CCAGCCGCTC 
CGGACCGACC TGCCCACGCA CTACGCCGGC GACGTGGACG GCGTCCCCGT GGACCTGCCC 
CTCCCGCCGC CTCCGGCTGC CGCGGGCCAG GGACTCGCGG GGGGCCAAGG GGCCGCCGAG 
CGGAAGAAGC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCGCG CCTGGAGGAG 
GAGCGTGAGC GCAAGCGGCG CGAGCAGGAG CGCAAGCTGG GCCAGATGCG CTCGCAGGCG 
CTGCTCCCCG CCCCGCTCTC CCCCGGCGCC ACCAAGCCCG CCGCCCCGGG GCCCCAGGAC 
ACCGTCATCC GGGAGCTGCA GCCTCAGCAG CAGCCGCGCA CCATCGAGCG CAGGGACCTG 
CAGTACATCA CCGTCAGCCG GGAGGAGCTC TCGGCCGGCG ACAGCCTGTC CCCCGACCCC 
TGGAAGCGCG ACGCGCGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACGT GGTGGACCTG 
CTGAGCCGGG AGATTCACGA GCTGCAGAGC AAGGCCGAGC GCTCGGCGGA GGAGAGCGGC 
CGCCTGCGCA AGCTCATGCT GGAGTGGCAG TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG 
AAGGACGAGG ACGACGACGA GGAGGAGGAC GACGACGTGG ACACCATGCT GATCATGCAG 
CGCCTGGAGG CCGAGCGCAG AGCGCGGCAG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCCCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839726.1
CDS	22..5514
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984272.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984272.2

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GGAAGTAGAC 
CAGGTGGCTG AGAAACAGAA GAACATCGCG GGGGCGCTTG CGTTCTGGAT GGCCAACGCG 
TCGGAACTCC TCAACTTCAT CAAGCAGGAC CGAGACCTCA GTCGAATCAC ACTGGACGCC 
CAGGATGTTT TGGCTCACCT GGTTCAGATG GCCTTTAAAT ACCTGGTTCA CTGTCTTCAG 
TCAGAGCTGA ACAACTACAT GCCGGCCTTT CTGGACGACC CCGAGGAGAA CAGTCTGCAA 
AGACCAAAAA TAGAGGACGT CCTCCACACG CTCACGGGCG CCATGTCGCT GCTGCGGCGC 
TGCCGGGTCA ACGCCGCCCT CACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCATCAAC 
ATGTGGCTGT TCAACAGGCT GCTGACCGAC CCCGAGTCGG GCCTGTGCTC CCACTACTGG 
GGCGCCATCG TCCGCCAGCA GCTGGGCCAC GTGGAGGCCT GGGCGGAGAA GCAGGGCCTG 
GAGCTGGCTG CCGACTGCCA CCTCAGCAGG GTCGTGCAGG CGACCACCCT GCTCACGATG 
GACAAGTACG CCCCGGAGGA CGCGGCCGGC ATCAACAGCA CCTGCTTCAA GCTGAACTCG 
CTGCAGCTGC AGGCCTTGCT GCACAACTAC CACTGTGCGC CCGACGAGCC CCTGGTCCCG 
CCGGATCTGA TAGAAAGCGT GGTGGCCGTG GCTGAAAACA CGGCGGACGA GCTGGCGCGC 
AGCGACGGCA GGGAGGTGCG GCTGGAGGAG GACCCTGACC TGCAGCTGCC CTTCCTGCTG 
CCGGAGGACG GCTACTCGTG TGACGTCGTC AGAAACATCC CCAACGGCTT GCAGGAGTTT 
TTGGATCCTT TGTGCCAGAG AGGGTTTTGC AGGCTGACGC CCCAGCCGCG CTCTCCGGGT 
TCCTGGACGA TACATTTTGA AGGTGCGGAG TACGAGAGCC GCCTCGCACG GGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCC GAAGTCATCA CTGTGACCCT GAAGAAGCAG 
AACGGGATGG GCCTCAGCAT CGTCGCAGCG AAGGGCGCAG GTCAAGACAA ACTCGGGATC 
TACGTCAAGT CGGTGGTGAG AGGAGGAGCT GCAGACGTGG ACGGGCGGCT GGCGGCGGGG 
GACCAGCTCC TCAGTGTGGA CGGACGAAGC CTGGTGGGCC TCTCTCAGGA AAGGGCAGCA 
GAGCTGATGA CAAGGACAAG TTCCGTGGTG ACCCTGGAGG TAGCGAAGCA GGGAGCCATT 
TACCACGGCC TCGCCACCCT GCTCAACCAG CCCTCGCCCA TGATGCAGAG AATTTCAGAC 
CGGCGTGGAT CAGGTAAACC CCGACCAAAG AGCGAAGGTT TTGAGCTCTA TAATAACTCA 
GCTCAGAACG GGTCCCCCGA GAGCCCCCCG CTGCCCTGGG CCGAGTACAG CGAGCCAAAG 
AAGCTGCCTG GCGACGAGAG GCTGACGAAG AACCGCGCCG ACCACCGCTC CAGCCCCAAC 
GTTGCAAATC AGCCTCCTAG TCCTGGTGGG AAAGGTGCAC ATGCCGCTGG AACAGCAGCC 
AAGATCACAT CCGTCTCCAC TGGCAACCTC TGCACCGAGG AGCAGACGCC CCCGCCGGGG 
ACGGAGGCCT ACCCCATCCC CACGCAGGCC TACCCCCGAG AGTACTTCAC CTTCCCGGCC 
TCCAGGTCCC AGGACCGCGT GGTCCCCCCC CAGAGCCAGT GGCCAAGCTA CGAGGAGAAG 
CCGCACGCGG ACAGCAGCCA CTCCAGCCTC GCGGTTCAGC GGGTGACCCG CTCCCAGGAG 
GAGCTGCGGG AGGACGCGGG GGCCTACCCA CTGCACAGGA TGGAGGCGGT GATGGACCGC 
AAGTCTGACA GCGACATGTG GGTCGCCCAG AGCTCCTCGA TGGGCTCCAG CGCGTCCAGC 
CAGGAGCACC TGGACCCCTC TTCCAAGGCG GGCACCCCCG CGTCCACCCT CACCAAGAGC 
GGCCCGGGGC GCTGGAAGAC CCCTGCAGTT CTGCAGCCCG CGCCCGGCCC CCAGCCGCTC 
CGGACCGACC TGCCCACGCA CTACGCCGGC GACGTGGACG GCGTCCCCGT GGACCTGCCC 
CTCCCGCCGC CTCCGGCTGC CGCGGGCCAG GGACTCGCGG GGGGCCAAGG GGCCGCCGAG 
CGGAAGAAGC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCGCG CCTGGAGGAG 
GAGCGTGAGC GCAAGCGGCG CGAGCAGGAG CGCAAGCTGG GCCAGATGCG CTCGCAGGCG 
CTGCTCCCCG CCCCGCTCTC CCCCGGCGCC ACCAAGCCCG CCGCCCCGGG GCCCCAGGAC 
ACCGTCATCC GGGAGCTGCA GCCTCAGCAG CAGCCGCGCA CCATCGAGCG CAGGGACCTG 
CAGTACATCA CCGTCAGCCG GGAGGAGCTC TCGGCCGGCG ACAGCCTGTC CCCCGACCCC 
TGGAAGCGCG ACGCGCGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACGT GGTGGACCTG 
CTGAGCCGGG AGATTCACGA GCTGCAGAGC AAGGCCGAGC GCTCGGCGGA GGAGAGCGGC 
CGCCTGCGCA AGCTCATGCT GGAGTGGCAG TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG 
AAGGACGAGG ACGACGACGA GGAGGAGGAC GACGACGTGG ACACCATGCT GATCATGCAG 
CGCCTGGAGG CCGAGCGCAG AGCGCGGCAG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCCCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984277.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839731.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

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CloneID	OSe229406
Clone ID Related Accession (Same CDS sequence)	XM_013984277.2
Accession Version	XM_013984277.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013984277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GGAAGTAGAC 
CAGGTGGCTG AGAAACAGAA GAACATCGCG GGGGCGCTTG CGTTCTGGAT GGCCAACGCG 
TCGGAACTCC TCAACTTCAT CAAGCAGGAC CGAGACCTCA GTCGAATCAC ACTGGACGCC 
CAGGATGTTT TGGCTCACCT GGTTCAGATG GCCTTTAAAT ACCTGGTTCA CTGTCTTCAG 
TCAGAGCTGA ACAACTACAT GCCGGCCTTT CTGGACGACC CCGAGGAGAA CAGTCTGCAA 
AGACCAAAAA TAGAGGACGT CCTCCACACG CTCACGGGCG CCATGTCGCT GCTGCGGCGC 
TGCCGGGTCA ACGCCGCCCT CACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCATCAAC 
ATGTGGCTGT TCAACAGGCT GCTGACCGAC CCCGAGTCGG GCCTGTGCTC CCACTACTGG 
GGCGCCATCG TCCGCCAGCA GCTGGGCCAC GTGGAGGCCT GGGCGGAGAA GCAGGGCCTG 
GAGCTGGCTG CCGACTGCCA CCTCAGCAGG GTCGTGCAGG CGACCACCCT GCTCACGATG 
GACAAGTACG CCCCGGAGGA CGCGGCCGGC ATCAACAGCA CCTGCTTCAA GCTGAACTCG 
CTGCAGCTGC AGGCCTTGCT GCACAACTAC CACTGTGCGC CCGACGAGCC CCTGGTCCCG 
CCGGATCTGA TAGAAAGCGT GGTGGCCGTG GCTGAAAACA CGGCGGACGA GCTGGCGCGC 
AGCGACGGCA GGGAGGTGCG GCTGGAGGAG GACCCTGACC TGCAGCTGCC CTTCCTGCTG 
CCGGAGGACG GCTACTCGTG TGACGTCGTC AGAAACATCC CCAACGGCTT GCAGGAGTTT 
TTGGATCCTT TGTGCCAGAG AGGGTTTTGC AGGCTGACGC CCCAGCCGCG CTCTCCGGGT 
TCCTGGACGA TACATTTTGA AGGTGCGGAG TACGAGAGCC GCCTCGCACG GGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCC GAAGTCATCA CTGTGACCCT GAAGAAGCAG 
AACGGGATGG GCCTCAGCAT CGTCGCAGCG AAGGGCGCAG GTCAAGACAA ACTCGGGATC 
TACGTCAAGT CGGTGGTGAG AGGAGGAGCT GCAGACGTGG ACGGGCGGCT GGCGGCGGGG 
GACCAGCTCC TCAGTGTGGA CGGACGAAGC CTGGTGGGCC TCTCTCAGGA AAGGGCAGCA 
GAGCTGATGA CAAGGACAAG TTCCGTGGTG ACCCTGGAGG TAGCGAAGCA GGGAGCCATT 
TACCACGGCC TCGCCACCCT GCTCAACCAG CCCTCGCCCA TGATGCAGAG AATTTCAGAC 
CGGCGTGGAT CAGGTAAACC CCGACCAAAG AGCGAAGGTT TTGAGCTCTA TAATAACTCA 
GCTCAGAACG GGTCCCCCGA GAGCCCCCCG CTGCCCTGGG CCGAGTACAG CGAGCCAAAG 
AAGCTGCCTG GCGACGAGAG GCTGACGAAG AACCGCGCCG ACCACCGCTC CAGCCCCAAC 
GTTGCAAATC AGCCTCCTAG TCCTGGTGGG AAAGGTGCAC ATGCCGCTGG AACAGCAGCC 
AAGATCACAT CCGTCTCCAC TGGCAACCTC TGCACCGAGG AGCAGACGCC CCCGCCGGGG 
ACGGAGGCCT ACCCCATCCC CACGCAGGCC TACCCCCGAG AGTACTTCAC CTTCCCGGCC 
TCCAGGTCCC AGGACCGCGT GGTCCCCCCC CAGAGCCAGT GGCCAAGCTA CGAGGAGAAG 
CCGCACGCGG ACAGCAGCCA CTCCAGCCTC GCGGTTCAGC GGGTGACCCG CTCCCAGGAG 
GAGCTGCGGG AGGACGCGGG GGCCTACCCA CTGCACAGGA TGGAGGCGGT GATGGACCGC 
AAGTCTGACA GCGACATGTG GGTCGCCCAG AGCTCCTCGA TGGGCTCCAG CGCGTCCAGC 
CAGGAGCACC TGGACCCCTC TTCCAAGGCG GGCACCCCCG CGTCCACCCT CACCAAGAGC 
GGCCCGGGGC GCTGGAAGAC CCCTGCAGTT CTGCAGCCCG CGCCCGGCCC CCAGCCGCTC 
CGGACCGACC TGCCCACGCA CTACGCCGGC GACGTGGACG GCGTCCCCGT GGACCTGCCC 
CTCCCGCCGC CTCCGGCTGC CGCGGGCCAG GGACTCGCGG GGGGCCAAGG GGCCGCCGAG 
CGGAAGAAGC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCGCG CCTGGAGGAG 
GAGCGTGAGC GCAAGCGGCG CGAGCAGGAG CGCAAGCTGG GCCAGATGCG CTCGCAGGCG 
CTGCTCCCCG CCCCGCTCTC CCCCGGCGCC ACCAAGCCCG CCGCCCCGGG GCCCCAGGAC 
ACCGTCATCC GGGAGCTGCA GCCTCAGCAG CAGCCGCGCA CCATCGAGCG CAGGGACCTG 
CAGTACATCA CCGTCAGCCG GGAGGAGCTC TCGGCCGGCG ACAGCCTGTC CCCCGACCCC 
TGGAAGCGCG ACGCGCGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACGT GGTGGACCTG 
CTGAGCCGGG AGATTCACGA GCTGCAGAGC AAGGCCGAGC GCTCGGCGGA GGAGAGCGGC 
CGCCTGCGCA AGCTCATGCT GGAGTGGCAG TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG 
AAGGACGAGG ACGACGACGA GGAGGAGGAC GACGACGTGG ACACCATGCT GATCATGCAG 
CGCCTGGAGG CCGAGCGCAG AGCGCGGCAG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCCCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839731.1
CDS	22..5514
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984277.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984277.2

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GGAAGTAGAC 
CAGGTGGCTG AGAAACAGAA GAACATCGCG GGGGCGCTTG CGTTCTGGAT GGCCAACGCG 
TCGGAACTCC TCAACTTCAT CAAGCAGGAC CGAGACCTCA GTCGAATCAC ACTGGACGCC 
CAGGATGTTT TGGCTCACCT GGTTCAGATG GCCTTTAAAT ACCTGGTTCA CTGTCTTCAG 
TCAGAGCTGA ACAACTACAT GCCGGCCTTT CTGGACGACC CCGAGGAGAA CAGTCTGCAA 
AGACCAAAAA TAGAGGACGT CCTCCACACG CTCACGGGCG CCATGTCGCT GCTGCGGCGC 
TGCCGGGTCA ACGCCGCCCT CACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCATCAAC 
ATGTGGCTGT TCAACAGGCT GCTGACCGAC CCCGAGTCGG GCCTGTGCTC CCACTACTGG 
GGCGCCATCG TCCGCCAGCA GCTGGGCCAC GTGGAGGCCT GGGCGGAGAA GCAGGGCCTG 
GAGCTGGCTG CCGACTGCCA CCTCAGCAGG GTCGTGCAGG CGACCACCCT GCTCACGATG 
GACAAGTACG CCCCGGAGGA CGCGGCCGGC ATCAACAGCA CCTGCTTCAA GCTGAACTCG 
CTGCAGCTGC AGGCCTTGCT GCACAACTAC CACTGTGCGC CCGACGAGCC CCTGGTCCCG 
CCGGATCTGA TAGAAAGCGT GGTGGCCGTG GCTGAAAACA CGGCGGACGA GCTGGCGCGC 
AGCGACGGCA GGGAGGTGCG GCTGGAGGAG GACCCTGACC TGCAGCTGCC CTTCCTGCTG 
CCGGAGGACG GCTACTCGTG TGACGTCGTC AGAAACATCC CCAACGGCTT GCAGGAGTTT 
TTGGATCCTT TGTGCCAGAG AGGGTTTTGC AGGCTGACGC CCCAGCCGCG CTCTCCGGGT 
TCCTGGACGA TACATTTTGA AGGTGCGGAG TACGAGAGCC GCCTCGCACG GGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCC GAAGTCATCA CTGTGACCCT GAAGAAGCAG 
AACGGGATGG GCCTCAGCAT CGTCGCAGCG AAGGGCGCAG GTCAAGACAA ACTCGGGATC 
TACGTCAAGT CGGTGGTGAG AGGAGGAGCT GCAGACGTGG ACGGGCGGCT GGCGGCGGGG 
GACCAGCTCC TCAGTGTGGA CGGACGAAGC CTGGTGGGCC TCTCTCAGGA AAGGGCAGCA 
GAGCTGATGA CAAGGACAAG TTCCGTGGTG ACCCTGGAGG TAGCGAAGCA GGGAGCCATT 
TACCACGGCC TCGCCACCCT GCTCAACCAG CCCTCGCCCA TGATGCAGAG AATTTCAGAC 
CGGCGTGGAT CAGGTAAACC CCGACCAAAG AGCGAAGGTT TTGAGCTCTA TAATAACTCA 
GCTCAGAACG GGTCCCCCGA GAGCCCCCCG CTGCCCTGGG CCGAGTACAG CGAGCCAAAG 
AAGCTGCCTG GCGACGAGAG GCTGACGAAG AACCGCGCCG ACCACCGCTC CAGCCCCAAC 
GTTGCAAATC AGCCTCCTAG TCCTGGTGGG AAAGGTGCAC ATGCCGCTGG AACAGCAGCC 
AAGATCACAT CCGTCTCCAC TGGCAACCTC TGCACCGAGG AGCAGACGCC CCCGCCGGGG 
ACGGAGGCCT ACCCCATCCC CACGCAGGCC TACCCCCGAG AGTACTTCAC CTTCCCGGCC 
TCCAGGTCCC AGGACCGCGT GGTCCCCCCC CAGAGCCAGT GGCCAAGCTA CGAGGAGAAG 
CCGCACGCGG ACAGCAGCCA CTCCAGCCTC GCGGTTCAGC GGGTGACCCG CTCCCAGGAG 
GAGCTGCGGG AGGACGCGGG GGCCTACCCA CTGCACAGGA TGGAGGCGGT GATGGACCGC 
AAGTCTGACA GCGACATGTG GGTCGCCCAG AGCTCCTCGA TGGGCTCCAG CGCGTCCAGC 
CAGGAGCACC TGGACCCCTC TTCCAAGGCG GGCACCCCCG CGTCCACCCT CACCAAGAGC 
GGCCCGGGGC GCTGGAAGAC CCCTGCAGTT CTGCAGCCCG CGCCCGGCCC CCAGCCGCTC 
CGGACCGACC TGCCCACGCA CTACGCCGGC GACGTGGACG GCGTCCCCGT GGACCTGCCC 
CTCCCGCCGC CTCCGGCTGC CGCGGGCCAG GGACTCGCGG GGGGCCAAGG GGCCGCCGAG 
CGGAAGAAGC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCGCG CCTGGAGGAG 
GAGCGTGAGC GCAAGCGGCG CGAGCAGGAG CGCAAGCTGG GCCAGATGCG CTCGCAGGCG 
CTGCTCCCCG CCCCGCTCTC CCCCGGCGCC ACCAAGCCCG CCGCCCCGGG GCCCCAGGAC 
ACCGTCATCC GGGAGCTGCA GCCTCAGCAG CAGCCGCGCA CCATCGAGCG CAGGGACCTG 
CAGTACATCA CCGTCAGCCG GGAGGAGCTC TCGGCCGGCG ACAGCCTGTC CCCCGACCCC 
TGGAAGCGCG ACGCGCGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACGT GGTGGACCTG 
CTGAGCCGGG AGATTCACGA GCTGCAGAGC AAGGCCGAGC GCTCGGCGGA GGAGAGCGGC 
CGCCTGCGCA AGCTCATGCT GGAGTGGCAG TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG 
AAGGACGAGG ACGACGACGA GGAGGAGGAC GACGACGTGG ACACCATGCT GATCATGCAG 
CGCCTGGAGG CCGAGCGCAG AGCGCGGCAG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCCCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_021085776.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020941435.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

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CloneID	OSe229407
Clone ID Related Accession (Same CDS sequence)	XM_021085776.1
Accession Version	XM_021085776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGGC TCAAAGTAAC 
GGACCTGAAA AGCAGGAAAA AGAAGGCGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG 
AAAGAAAAGA AGGAAAAAAA GAAGAGAGAG AAAGAGGCGA TGAGACAAGC ATCTGATAAA 
GATGATAGAC CCTTCCACGG GGAGGACACT GAGAATTCTC GACTGGCTGC CGAGGTTTAC 
AAAGACATGC CCGAAACTAG CTTTACCCGA ACAATCTCCA ACCCCGAGGT GGTCATGAAG 
CGAAGGCGGC AGCAAAAACT GGAAAAGAGG ATGCAGGAGT TTCGGAGCTC AGACGGGCGC 
CCTGATTCCG GTGGAACGTT GAGAATTTAT GCGGATAGTT TAAAGCCAAA TATTCCCTAC 
AAGACAATCC TGCTGTCCAC CACGGACCCC GCCGACTTTG CCGTGGCGGA GGCTTTGGAG 
AAGTACGGTC TGGAGAAGGA GAACCCGAAG GATTACTGCA TCGCCCGGGT TATGCTTCCT 
CCTGGAGCCC AGCATTCGGA TGAGAAAGGT GCTAAAGAAA TTATCCTGGA CGATGACGAG 
TGTCCTTTAC AGATCTTCAG GGAATGGCCT GGTGACAAAG GGATGCTGGT CTTCCAGCTG 
AAGAGGAGGC CCCCAGAGCA CATCCCCAAG AAGAGCAAGA AGCACGCGGA GGGGAAGCCG 
CCCAAGGGGA AGGAGCGGCC CGACGGCTCT GGCTATGGCT CTGCGCTCCC CCCGGAGAAG 
CTGCCCTACC TAGTGGAGCT GAGCCCAGGG AGAAGGGGTC ACTTTGCCTA CTACAGCTAT 
CACCCTCACG AAGACGGCTC CGACTCCAGA GATAAGCCAA AGCTTTACCG CCTCCAGTTA 
AGCGTCACCG AGGTCGGGAC GGAGAAGTTC GATGACAGCT CGATCCAGTT GTTTGGTTCC 
GGAATCCAGC CCCACCACTG CGACCTCACC AACATGGACG GGGTGGTCAC TGTCACGCCG 
AGAAGCGTGG ATGCTGAGAC CTACGTGGAA GGACAGCGCA TCTCGGAGAC GACCATGCTG 
CAGAGCGGGG TGAAGGTGCA GTTCGGGGCG TCCCACGTGT TCAAGTTTGT GGACCCCAGC 
CAGGACCACG CGCTCGCCAA GAGGCCGGGG GACGGAGGCC TGGCCGTCAA GGGTCCCAGA 
CACAAACCGG GAATCGTTCA GGAGACAGCT TTCGATTTAG GAGGAGACGT TCACGGGGGG 
ACGGCCGTAG CGAGCAGTAA GGGCTCCGCC CGACCGGACG GCGACAGGGC CCCGTCTGCC 
TCCAGCAGCG CCGAGCGCGG GATGGTGAAG CCGATGGTGC GAATGGAGCC GCCGCAGGAG 
CCCCGCAGGC AGGACACCAG ACCGCAGGAC GCCGCTGGGC CTGAGCTGAC GCTGCCCGCG 
AGCATCGAAT TCAGGGAAAG TGCTGAAGAC TCGTTCTTAT CCGCCATCAT CAATTACACG 
AATAGCTCGA CGGTGCATTT TAAGTTGTCG CCTACGTACG TGCTGTACAT GGCGTGTCGG 
CACGTGCTGT CCAGCCAGTA CCGCCCGGAC GTCAGCCCCG CCGAGCGCAC GCACAAGGTC 
ATCGCCATCG TCAACAAGAT GGTGAGCATG ATGGACGGCG TCATCCAGGA AGTAGACCAG 
GTGGCTGAGA AACAGAAGAA CATCGCGGGG GCGCTTGCGT TCTGGATGGC CAACGCGTCG 
GAACTCCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GAATCACACT GGACGCCCAG 
GATGTTTTGG CTCACCTGGT TCAGATGGCC TTTAAATACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAACA ACTACATGCC GGCCTTTCTG GACGACCCCG AGGAGAACAG TCTGCAAAGA 
CCAAAAATAG AGGACGTCCT CCACACGCTC ACGGGCGCCA TGTCGCTGCT GCGGCGCTGC 
CGGGTCAACG CCGCCCTCAC CATCCAGCTC TTCTCCCAGC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACAGGCTGCT GACCGACCCC GAGTCGGGCC TGTGCTCCCA CTACTGGGGC 
GCCATCGTCC GCCAGCAGCT GGGCCACGTG GAGGCCTGGG CGGAGAAGCA GGGCCTGGAG 
CTGGCTGCCG ACTGCCACCT CAGCAGGGTC GTGCAGGCGA CCACCCTGCT CACGATGGAC 
AAGTACGCCC CGGAGGACGC GGCCGGCATC AACAGCACCT GCTTCAAGCT GAACTCGCTG 
CAGCTGCAGG CCTTGCTGCA CAACTACCAC TGTGCGCCCG ACGAGCCCCT GGTCCCGCCG 
GATCTGATAG AAAGCGTGGT GGCCGTGGCT GAAAACACGG CGGACGAGCT GGCGCGCAGC 
GACGGCAGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCCTT CCTGCTGCCG 
GAGGACGGCT ACTCGTGTGA CGTCGTCAGA AACATCCCCA ACGGCTTGCA GGAGTTTTTG 
GATCCTTTGT GCCAGAGAGG GTTTTGCAGG CTGACGCCCC AGCCGCGCTC TCCGGGTTCC 
TGGACGATAC ATTTTGAAGG TGCGGAGTAC GAGAGCCGCC TCGCACGGGA GAGCACGGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCCGAA GTCATCACTG TGACCCTGAA GAAGCAGAAC 
GGGATGGGCC TCAGCATCGT CGCAGCGAAG GGCGCAGGTC AAGACAAACT CGGGATCTAC 
GTCAAGTCGG TGGTGAGAGG AGGAGCTGCA GACGTGGACG GGCGGCTGGC GGCGGGGGAC 
CAGCTCCTCA GTGTGGACGG ACGAAGCCTG GTGGGCCTCT CTCAGGAAAG GGCAGCAGAG 
CTGATGACAA GGACAAGTTC CGTGGTGACC CTGGAGGTAG CGAAGCAGGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCC TCGCCCATGA TGCAGAGAAT TTCAGACCGG 
CGTGGATCAG GTAAACCCCG ACCAAAGAGC GAAGGTTTTG AGCTCTATAA TAACTCAGCT 
CAGAACGGGT CCCCCGAGAG CCCCCCGCTG CCCTGGGCCG AGTACAGCGA GCCAAAGAAG 
CTGCCTGGCG ACGAGAGGCT GACGAAGAAC CGCGCCGACC ACCGCTCCAG CCCCAACGTT 
GCAAATCAGC CTCCTAGTCC TGGTGGGAAA GGTGCACATG CCGCTGGAAC AGCAGCCAAG 
ATCACATCCG TCTCCACTGG CAACCTCTGC ACCGAGGAGC AGACGCCCCC GCCGGGGACG 
GAGGCCTACC CCATCCCCAC GCAGGCCTAC CCCCGAGAGT ACTTCACCTT CCCGGCCTCC 
AGGTCCCAGG ACCGCGTGGT CCCCCCCCAG AGCCAGTGGC CAAGCTACGA GGAGAAGCCG 
CACGCGGACA GCAGCCACTC CAGCCTCGCG GTTCAGCGGG TGACCCGCTC CCAGGAGGAG 
CTGCGGGAGG ACGCGGGGGC CTACCCACTG CACAGGATGG AGGCGGTGAT GGACCGCAAG 
TCTGACAGCG ACATGTGGGT CGCCCAGAGC TCCTCGATGG GCTCCAGCGC GTCCAGCCAG 
GAGCACCTGG ACCCCTCTTC CAAGGCGGGC ACCCCCGCGT CCACCCTCAC CAAGAGCGGC 
CCGGGGCGCT GGAAGACCCC TGCAGTTCTG CAGCCCGCGC CCGGCCCCCA GCCGCTCCGG 
ACCGACCTGC CCACGCACTA CGCCGGCGAC GTGGACGGCG TCCCCGTGGA CCTGCCCCTC 
CCGCCGCCTC CGGCTGCCGC GGGCCAGGGA CTCGCGGGGG GCCAAGGGGC CGCCGAGCGG 
AAGAAGCGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCGCGCCT GGAGGAGGAG 
CGTGAGCGCA AGCGGCGCGA GCAGGAGCGC AAGCTGGGCC AGATGCGCTC GCAGGCGCTG 
CTCCCCGCCC CGCTCTCCCC CGGCGCCACC AAGCCCGCCG CCCCGGGGCC CCAGGACACC 
GTCATCCGGG AGCTGCAGCC TCAGCAGCAG CCGCGCACCA TCGAGCGCAG GGACCTGCAG 
TACATCACCG TCAGCCGGGA GGAGCTCTCG GCCGGCGACA GCCTGTCCCC CGACCCCTGG 
AAGCGCGACG CGCGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACGTGGT GGACCTGCTG 
AGCCGGGAGA TTCACGAGCT GCAGAGCAAG GCCGAGCGCT CGGCGGAGGA GAGCGGCCGC 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGCGGC TGCAGGAGTC CAAGCAGAAG 
GACGAGGACG ACGACGAGGA GGAGGACGAC GACGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCCG AGCGCAGAGC GCGGCAGACT GCTGTGCCAG CAATCTCTGT TCTAGATTTG 
TTGCAGGACG AGGAGCGGCG GCGGCAGCAG CAGCTGGAGG AGATGCGGCA GCGGGAGGCG 
GAGGAGCGTG CCCGCCACGA GGAGGAGCGG CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC 
GAGGCCGAGG AAAAGAGGCG CCAGGAGGAG GGGTACTACA GCCGCCTGGA GGCCGAGCGG 
CGCAGACAGC ACGAGGAGGC CGAGCGCCGG CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC 
CGCCCCCCGC TCCCGCGGGG CTACGAGCCC CCCGGCCCCG CGCCGCCGCC CCCGCCCCAG 
CGCGCCGCCT CCTACCTGCA GGCCCAGGTC CTGTCCCCCG ACTCCCTGTA CACCGCCAAG 
TTCGTGGCTT ACGACGACGA TGACGAGGAG GAGGACGGCC CTGCAGGTCA GGATAAGTAC 
GCCCGCGCCA GGGGGGCCCG CGGGGGCCCC CGCCCGGCCT CCGATGGCAT CTTTCTCGGC 
AGCTCCGCCC AGGCGCCCTG GGCCAGGGCC AACAGTCCTG CGCACAGGGC AGGCGCGCCC 
CCGGCCCCGC CGCCCAAGCC CGGCCCCGCC CGAGGCCGAG GGCCCGCCTC GTACATGGGA 
TCTACGGGGA CGCTGATGGG CGCCCACGAT GGCTCTCGGG ACCCAAGAGA GACGCTGTCC 
AAAAGCCAGG ACGCGGACGT GCCGGGCGGG CCTGAGAGCC TGACCTTCCG GGAGCGCCAG 
CGCCTCTTCT CCCAGGGCCA CGAGGTCTCC AGTAAGGTGA AAGCCTCCCG CCGGCTGACG 
GAGCTGGAGA ACGAGCTGAA CACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020941435.1
CDS	22..5511
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_021085776.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021085776.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGGC TCAAAGTAAC 
GGACCTGAAA AGCAGGAAAA AGAAGGCGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG 
AAAGAAAAGA AGGAAAAAAA GAAGAGAGAG AAAGAGGCGA TGAGACAAGC ATCTGATAAA 
GATGATAGAC CCTTCCACGG GGAGGACACT GAGAATTCTC GACTGGCTGC CGAGGTTTAC 
AAAGACATGC CCGAAACTAG CTTTACCCGA ACAATCTCCA ACCCCGAGGT GGTCATGAAG 
CGAAGGCGGC AGCAAAAACT GGAAAAGAGG ATGCAGGAGT TTCGGAGCTC AGACGGGCGC 
CCTGATTCCG GTGGAACGTT GAGAATTTAT GCGGATAGTT TAAAGCCAAA TATTCCCTAC 
AAGACAATCC TGCTGTCCAC CACGGACCCC GCCGACTTTG CCGTGGCGGA GGCTTTGGAG 
AAGTACGGTC TGGAGAAGGA GAACCCGAAG GATTACTGCA TCGCCCGGGT TATGCTTCCT 
CCTGGAGCCC AGCATTCGGA TGAGAAAGGT GCTAAAGAAA TTATCCTGGA CGATGACGAG 
TGTCCTTTAC AGATCTTCAG GGAATGGCCT GGTGACAAAG GGATGCTGGT CTTCCAGCTG 
AAGAGGAGGC CCCCAGAGCA CATCCCCAAG AAGAGCAAGA AGCACGCGGA GGGGAAGCCG 
CCCAAGGGGA AGGAGCGGCC CGACGGCTCT GGCTATGGCT CTGCGCTCCC CCCGGAGAAG 
CTGCCCTACC TAGTGGAGCT GAGCCCAGGG AGAAGGGGTC ACTTTGCCTA CTACAGCTAT 
CACCCTCACG AAGACGGCTC CGACTCCAGA GATAAGCCAA AGCTTTACCG CCTCCAGTTA 
AGCGTCACCG AGGTCGGGAC GGAGAAGTTC GATGACAGCT CGATCCAGTT GTTTGGTTCC 
GGAATCCAGC CCCACCACTG CGACCTCACC AACATGGACG GGGTGGTCAC TGTCACGCCG 
AGAAGCGTGG ATGCTGAGAC CTACGTGGAA GGACAGCGCA TCTCGGAGAC GACCATGCTG 
CAGAGCGGGG TGAAGGTGCA GTTCGGGGCG TCCCACGTGT TCAAGTTTGT GGACCCCAGC 
CAGGACCACG CGCTCGCCAA GAGGCCGGGG GACGGAGGCC TGGCCGTCAA GGGTCCCAGA 
CACAAACCGG GAATCGTTCA GGAGACAGCT TTCGATTTAG GAGGAGACGT TCACGGGGGG 
ACGGCCGTAG CGAGCAGTAA GGGCTCCGCC CGACCGGACG GCGACAGGGC CCCGTCTGCC 
TCCAGCAGCG CCGAGCGCGG GATGGTGAAG CCGATGGTGC GAATGGAGCC GCCGCAGGAG 
CCCCGCAGGC AGGACACCAG ACCGCAGGAC GCCGCTGGGC CTGAGCTGAC GCTGCCCGCG 
AGCATCGAAT TCAGGGAAAG TGCTGAAGAC TCGTTCTTAT CCGCCATCAT CAATTACACG 
AATAGCTCGA CGGTGCATTT TAAGTTGTCG CCTACGTACG TGCTGTACAT GGCGTGTCGG 
CACGTGCTGT CCAGCCAGTA CCGCCCGGAC GTCAGCCCCG CCGAGCGCAC GCACAAGGTC 
ATCGCCATCG TCAACAAGAT GGTGAGCATG ATGGACGGCG TCATCCAGGA AGTAGACCAG 
GTGGCTGAGA AACAGAAGAA CATCGCGGGG GCGCTTGCGT TCTGGATGGC CAACGCGTCG 
GAACTCCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GAATCACACT GGACGCCCAG 
GATGTTTTGG CTCACCTGGT TCAGATGGCC TTTAAATACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAACA ACTACATGCC GGCCTTTCTG GACGACCCCG AGGAGAACAG TCTGCAAAGA 
CCAAAAATAG AGGACGTCCT CCACACGCTC ACGGGCGCCA TGTCGCTGCT GCGGCGCTGC 
CGGGTCAACG CCGCCCTCAC CATCCAGCTC TTCTCCCAGC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACAGGCTGCT GACCGACCCC GAGTCGGGCC TGTGCTCCCA CTACTGGGGC 
GCCATCGTCC GCCAGCAGCT GGGCCACGTG GAGGCCTGGG CGGAGAAGCA GGGCCTGGAG 
CTGGCTGCCG ACTGCCACCT CAGCAGGGTC GTGCAGGCGA CCACCCTGCT CACGATGGAC 
AAGTACGCCC CGGAGGACGC GGCCGGCATC AACAGCACCT GCTTCAAGCT GAACTCGCTG 
CAGCTGCAGG CCTTGCTGCA CAACTACCAC TGTGCGCCCG ACGAGCCCCT GGTCCCGCCG 
GATCTGATAG AAAGCGTGGT GGCCGTGGCT GAAAACACGG CGGACGAGCT GGCGCGCAGC 
GACGGCAGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCCTT CCTGCTGCCG 
GAGGACGGCT ACTCGTGTGA CGTCGTCAGA AACATCCCCA ACGGCTTGCA GGAGTTTTTG 
GATCCTTTGT GCCAGAGAGG GTTTTGCAGG CTGACGCCCC AGCCGCGCTC TCCGGGTTCC 
TGGACGATAC ATTTTGAAGG TGCGGAGTAC GAGAGCCGCC TCGCACGGGA GAGCACGGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCCGAA GTCATCACTG TGACCCTGAA GAAGCAGAAC 
GGGATGGGCC TCAGCATCGT CGCAGCGAAG GGCGCAGGTC AAGACAAACT CGGGATCTAC 
GTCAAGTCGG TGGTGAGAGG AGGAGCTGCA GACGTGGACG GGCGGCTGGC GGCGGGGGAC 
CAGCTCCTCA GTGTGGACGG ACGAAGCCTG GTGGGCCTCT CTCAGGAAAG GGCAGCAGAG 
CTGATGACAA GGACAAGTTC CGTGGTGACC CTGGAGGTAG CGAAGCAGGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCC TCGCCCATGA TGCAGAGAAT TTCAGACCGG 
CGTGGATCAG GTAAACCCCG ACCAAAGAGC GAAGGTTTTG AGCTCTATAA TAACTCAGCT 
CAGAACGGGT CCCCCGAGAG CCCCCCGCTG CCCTGGGCCG AGTACAGCGA GCCAAAGAAG 
CTGCCTGGCG ACGAGAGGCT GACGAAGAAC CGCGCCGACC ACCGCTCCAG CCCCAACGTT 
GCAAATCAGC CTCCTAGTCC TGGTGGGAAA GGTGCACATG CCGCTGGAAC AGCAGCCAAG 
ATCACATCCG TCTCCACTGG CAACCTCTGC ACCGAGGAGC AGACGCCCCC GCCGGGGACG 
GAGGCCTACC CCATCCCCAC GCAGGCCTAC CCCCGAGAGT ACTTCACCTT CCCGGCCTCC 
AGGTCCCAGG ACCGCGTGGT CCCCCCCCAG AGCCAGTGGC CAAGCTACGA GGAGAAGCCG 
CACGCGGACA GCAGCCACTC CAGCCTCGCG GTTCAGCGGG TGACCCGCTC CCAGGAGGAG 
CTGCGGGAGG ACGCGGGGGC CTACCCACTG CACAGGATGG AGGCGGTGAT GGACCGCAAG 
TCTGACAGCG ACATGTGGGT CGCCCAGAGC TCCTCGATGG GCTCCAGCGC GTCCAGCCAG 
GAGCACCTGG ACCCCTCTTC CAAGGCGGGC ACCCCCGCGT CCACCCTCAC CAAGAGCGGC 
CCGGGGCGCT GGAAGACCCC TGCAGTTCTG CAGCCCGCGC CCGGCCCCCA GCCGCTCCGG 
ACCGACCTGC CCACGCACTA CGCCGGCGAC GTGGACGGCG TCCCCGTGGA CCTGCCCCTC 
CCGCCGCCTC CGGCTGCCGC GGGCCAGGGA CTCGCGGGGG GCCAAGGGGC CGCCGAGCGG 
AAGAAGCGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCGCGCCT GGAGGAGGAG 
CGTGAGCGCA AGCGGCGCGA GCAGGAGCGC AAGCTGGGCC AGATGCGCTC GCAGGCGCTG 
CTCCCCGCCC CGCTCTCCCC CGGCGCCACC AAGCCCGCCG CCCCGGGGCC CCAGGACACC 
GTCATCCGGG AGCTGCAGCC TCAGCAGCAG CCGCGCACCA TCGAGCGCAG GGACCTGCAG 
TACATCACCG TCAGCCGGGA GGAGCTCTCG GCCGGCGACA GCCTGTCCCC CGACCCCTGG 
AAGCGCGACG CGCGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACGTGGT GGACCTGCTG 
AGCCGGGAGA TTCACGAGCT GCAGAGCAAG GCCGAGCGCT CGGCGGAGGA GAGCGGCCGC 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGCGGC TGCAGGAGTC CAAGCAGAAG 
GACGAGGACG ACGACGAGGA GGAGGACGAC GACGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCCG AGCGCAGAGC GCGGCAGACT GCTGTGCCAG CAATCTCTGT TCTAGATTTG 
TTGCAGGACG AGGAGCGGCG GCGGCAGCAG CAGCTGGAGG AGATGCGGCA GCGGGAGGCG 
GAGGAGCGTG CCCGCCACGA GGAGGAGCGG CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC 
GAGGCCGAGG AAAAGAGGCG CCAGGAGGAG GGGTACTACA GCCGCCTGGA GGCCGAGCGG 
CGCAGACAGC ACGAGGAGGC CGAGCGCCGG CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC 
CGCCCCCCGC TCCCGCGGGG CTACGAGCCC CCCGGCCCCG CGCCGCCGCC CCCGCCCCAG 
CGCGCCGCCT CCTACCTGCA GGCCCAGGTC CTGTCCCCCG ACTCCCTGTA CACCGCCAAG 
TTCGTGGCTT ACGACGACGA TGACGAGGAG GAGGACGGCC CTGCAGGTCA GGATAAGTAC 
GCCCGCGCCA GGGGGGCCCG CGGGGGCCCC CGCCCGGCCT CCGATGGCAT CTTTCTCGGC 
AGCTCCGCCC AGGCGCCCTG GGCCAGGGCC AACAGTCCTG CGCACAGGGC AGGCGCGCCC 
CCGGCCCCGC CGCCCAAGCC CGGCCCCGCC CGAGGCCGAG GGCCCGCCTC GTACATGGGA 
TCTACGGGGA CGCTGATGGG CGCCCACGAT GGCTCTCGGG ACCCAAGAGA GACGCTGTCC 
AAAAGCCAGG ACGCGGACGT GCCGGGCGGG CCTGAGAGCC TGACCTTCCG GGAGCGCCAG 
CGCCTCTTCT CCCAGGGCCA CGAGGTCTCC AGTAAGGTGA AAGCCTCCCG CCGGCTGACG 
GAGCTGGAGA ACGAGCTGAA CACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_021085787.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020941446.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSe229408
Clone ID Related Accession (Same CDS sequence)	XM_021085787.1
Accession Version	XM_021085787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGACGAGAAG CCCCTAGTTG TGCAGCTGAA TTGGAATAAA 
GATGACCGCG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ACGCCGCTCC TCCCAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AGAAAGAGGC GATGAGACAA 
GCATCTGATA AAGATGATAG ACCCTTCCAC GGGGAGGACA CTGAGAATTC TCGACTGGCT 
GCCGAGGTTT ACAAAGACAT GCCCGAAACT AGCTTTACCC GAACAATCTC CAACCCCGAG 
GTGGTCATGA AGCGAAGGCG GCAGCAAAAA CTGGAAAAGA GGATGCAGGA GTTTCGGAGC 
TCAGACGGGC GCCCTGATTC CGGTGGAACG TTGAGAATTT ATGCGGATAG TTTAAAGCCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGACC CCGCCGACTT TGCCGTGGCG 
GAGGCTTTGG AGAAGTACGG TCTGGAGAAG GAGAACCCGA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCG GATGAGAAAG GTGCTAAAGA AATTATCCTG 
GACGATGACG AGTGTCCTTT ACAGATCTTC AGGGAATGGC CTGGTGACAA AGGGATGCTG 
GTCTTCCAGC TGAAGAGGAG GCCCCCAGAG CACATCCCCA AGAAGAGCAA GAAGCACGCG 
GAGGGGAAGC CGCCCAAGGG GAAGGAGCGG CCCGACGGCT CTGGCTATGG CTCTGCGCTC 
CCCCCGGAGA AGCTGCCCTA CCTAGTGGAG CTGAGCCCAG GGAGAAGGGG TCACTTTGCC 
TACTACAGCT ATCACCCTCA CGAAGACGGC TCCGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTCCAGT TAAGCGTCAC CGAGGTCGGG ACGGAGAAGT TCGATGACAG CTCGATCCAG 
TTGTTTGGTT CCGGAATCCA GCCCCACCAC TGCGACCTCA CCAACATGGA CGGGGTGGTC 
ACTGTCACGC CGAGAAGCGT GGATGCTGAG ACCTACGTGG AAGGACAGCG CATCTCGGAG 
ACGACCATGC TGCAGAGCGG GGTGAAGGTG CAGTTCGGGG CGTCCCACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCGCTCGCC AAGAGGCCGG GGGACGGAGG CCTGGCCGTC 
AAGGGTCCCA GACACAAACC GGGAATCGTT CAGGAGACAG CTTTCGATTT AGGAGGAGAC 
GTTCACGGGG GGACGGCCGT AGCGAGCAGT AAGGGCTCCG CCCGACCGGA CGGCGACAGG 
GCCCCGTCTG CCTCCAGCAG CGCCGAGCGC GGGATGGTGA AGCCGATGGT GCGAATGGAG 
CCGCCGCAGG AGCCCCGCAG GCAGGACACC AGACCGCAGG ACGCCGCTGG GCCTGAGCTG 
ACGCTGCCCG CGAGCATCGA ATTCAGGGAA AGTGCTGAAG ACTCGTTCTT ATCCGCCATC 
ATCAATTACA CGAATAGCTC GACGGTGCAT TTTAAGTTGT CGCCTACGTA CGTGCTGTAC 
ATGGCGTGTC GGCACGTGCT GTCCAGCCAG TACCGCCCGG ACGTCAGCCC CGCCGAGCGC 
ACGCACAAGG TCATCGCCAT CGTCAACAAG ATGGTGAGCA TGATGGACGG CGTCATCCAG 
GAAGTAGACC AGGTGGCTGA GAAACAGAAG AACATCGCGG GGGCGCTTGC GTTCTGGATG 
GCCAACGCGT CGGAACTCCT CAACTTCATC AAGCAGGACC GAGACCTCAG TCGAATCACA 
CTGGACGCCC AGGATGTTTT GGCTCACCTG GTTCAGATGG CCTTTAAATA CCTGGTTCAC 
TGTCTTCAGT CAGAGCTGAA CAACTACATG CCGGCCTTTC TGGACGACCC CGAGGAGAAC 
AGTCTGCAAA GACCAAAAAT AGAGGACGTC CTCCACACGC TCACGGGCGC CATGTCGCTG 
CTGCGGCGCT GCCGGGTCAA CGCCGCCCTC ACCATCCAGC TCTTCTCCCA GCTCTTCCAC 
TTCATCAACA TGTGGCTGTT CAACAGGCTG CTGACCGACC CCGAGTCGGG CCTGTGCTCC 
CACTACTGGG GCGCCATCGT CCGCCAGCAG CTGGGCCACG TGGAGGCCTG GGCGGAGAAG 
CAGGGCCTGG AGCTGGCTGC CGACTGCCAC CTCAGCAGGG TCGTGCAGGC GACCACCCTG 
CTCACGATGG ACAAGTACGC CCCGGAGGAC GCGGCCGGCA TCAACAGCAC CTGCTTCAAG 
CTGAACTCGC TGCAGCTGCA GGCCTTGCTG CACAACTACC ACTGTGCGCC CGACGAGCCC 
CTGGTCCCGC CGGATCTGAT AGAAAGCGTG GTGGCCGTGG CTGAAAACAC GGCGGACGAG 
CTGGCGCGCA GCGACGGCAG GGAGGTGCGG CTGGAGGAGG ACCCTGACCT GCAGCTGCCC 
TTCCTGCTGC CGGAGGACGG CTACTCGTGT GACGTCGTCA GAAACATCCC CAACGGCTTG 
CAGGAGTTTT TGGATCCTTT GTGCCAGAGA GGGTTTTGCA GGCTGACGCC CCAGCCGCGC 
TCTCCGGGTT CCTGGACGAT ACATTTTGAA GGTGCGGAGT ACGAGAGCCG CCTCGCACGG 
GAGAGCACGG AGCTGGCTCA GCCTCTGAGG AAAGAACCCG AAGTCATCAC TGTGACCCTG 
AAGAAGCAGA ACGGGATGGG CCTCAGCATC GTCGCAGCGA AGGGCGCAGG TCAAGACAAA 
CTCGGGATCT ACGTCAAGTC GGTGGTGAGA GGAGGAGCTG CAGACGTGGA CGGGCGGCTG 
GCGGCGGGGG ACCAGCTCCT CAGTGTGGAC GGACGAAGCC TGGTGGGCCT CTCTCAGGAA 
AGGGCAGCAG AGCTGATGAC AAGGACAAGT TCCGTGGTGA CCCTGGAGGT AGCGAAGCAG 
GGAGCCATTT ACCACGGCCT CGCCACCCTG CTCAACCAGC CCTCGCCCAT GATGCAGAGA 
ATTTCAGACC GGCGTGGATC AGGTAAACCC CGACCAAAGA GCGAAGGTTT TGAGCTCTAT 
AATAACTCAG CTCAGAACGG GTCCCCCGAG AGCCCCCCGC TGCCCTGGGC CGAGTACAGC 
GAGCCAAAGA AGCTGCCTGG CGACGAGAGG CTGACGAAGA ACCGCGCCGA CCACCGCTCC 
AGCCCCAACG TTGCAAATCA GCCTCCTAGT CCTGGTGGGA AAGGTGCACA TGCCGCTGGA 
ACAGCAGCCA AGATCACATC CGTCTCCACT GGCAACCTCT GCACCGAGGA GCAGACGCCC 
CCGCCGGGGA CGGAGGCCTA CCCCATCCCC ACGCAGGCCT ACCCCCGAGA GTACTTCACC 
TTCCCGGCCT CCAGGTCCCA GGACCGCGTG GTCCCCCCCC AGAGCCAGTG GCCAAGCTAC 
GAGGAGAAGC CGCACGCGGA CAGCAGCCAC TCCAGCCTCG CGGTTCAGCG GGTGACCCGC 
TCCCAGGAGG AGCTGCGGGA GGACGCGGGG GCCTACCCAC TGCACAGGAT GGAGGCGGTG 
ATGGACCGCA AGTCTGACAG CGACATGTGG GTCGCCCAGA GCTCCTCGAT GGGCTCCAGC 
GCGTCCAGCC AGGAGCACCT GGACCCCTCT TCCAAGGCGG GCACCCCCGC GTCCACCCTC 
ACCAAGAGCG GCCCGGGGCG CTGGAAGACC CCTGCAGTTC TGCAGCCCGC GCCCGGCCCC 
CAGCCGCTCC GGACCGACCT GCCCACGCAC TACGCCGGCG ACGTGGACGG CGTCCCCGTG 
GACCTGCCCC TCCCGCCGCC TCCGGCTGCC GCGGGCCAGG GACTCGCGGG GGGCCAAGGG 
GCCGCCGAGC GGAAGAAGCG GGAGGAGCAG CAGCGCTGGT ACGAGAAGGA GAAGGCGCGC 
CTGGAGGAGG AGCGTGAGCG CAAGCGGCGC GAGCAGGAGC GCAAGCTGGG CCAGATGCGC 
TCGCAGGCGC TGCTCCCCGC CCCGCTCTCC CCCGGCGCCA CCAAGCCCGC CGCCCCGGGG 
CCCCAGGACA CCGTCATCCG GGAGCTGCAG CCTCAGCAGC AGCCGCGCAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC CGTCAGCCGG GAGGAGCTCT CGGCCGGCGA CAGCCTGTCC 
CCCGACCCCT GGAAGCGCGA CGCGCGGGAG AAGCTGGAGA AGCAGCAGCA GATGCACGTG 
GTGGACCTGC TGAGCCGGGA GATTCACGAG CTGCAGAGCA AGGCCGAGCG CTCGGCGGAG 
GAGAGCGGCC GCCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG 
TCCAAGCAGA AGGACGAGGA CGACGACGAG GAGGAGGACG ACGACGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC CGAGCGCAGA GCGCGGCAGA CTGCTGTGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG CGGCGGCAGC AGCAGCTGGA GGAGATGCGG 
CAGCGGGAGG CGGAGGAGCG TGCCCGCCAC GAGGAGGAGC GGCGGCGCCA GGAGGAGGAG 
CGGCTGAAGC GCGAGGCCGA GGAAAAGAGG CGCCAGGAGG AGGGGTACTA CAGCCGCCTG 
GAGGCCGAGC GGCGCAGACA GCACGAGGAG GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG 
CCGGGCCTGG GCCGCCCCCC GCTCCCGCGG GGCTACGAGC CCCCCGGCCC CGCGCCGCCG 
CCCCCGCCCC AGCGCGCCGC CTCCTACCTG CAGGCCCAGG TCCTGTCCCC CGACTCCCTG 
TACACCGCCA AGTTCGTGGC TTACGACGAC GATGACGAGG AGGAGGACGG CCCTGCAGGT 
CAGGATAAGT ACGCCCGCGC CAGGGGGGCC CGCGGGGGCC CCCGCCCGGC CTCCGATGGC 
ATCTTTCTCG GCAGCTCCGC CCAGGCGCCC TGGGCCAGGG CCAACAGTCC TGCGCACAGG 
GCAGGCGCGC CCCCGGCCCC GCCGCCCAAG CCCGGCCCCG CCCGAGGCCG AGGGCCCGCC 
TCGTACATGG GATCTACGGG GACGCTGATG GGCGCCCACG ATGGCTCTCG GGACCCAAGA 
GAGACGCTGT CCAAAAGCCA GGACGCGGAC GTGCCGGGCG GGCCTGAGAG CCTGACCTTC 
CGGGAGCGCC AGCGCCTCTT CTCCCAGGGC CACGAGGTCT CCAGTAAGGT GAAAGCCTCC 
CGCCGGCTGA CGGAGCTGGA GAACGAGCTG AACACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020941446.1
CDS	28..5649
Translation	MSAGARDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_021085787.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021085787.1

ORF Insert Sequence:

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGACGAGAAG CCCCTAGTTG TGCAGCTGAA TTGGAATAAA 
GATGACCGCG AGGGCAGGTT TGTCCTTAAG AACGAGAACG ACGCCGCTCC TCCCAAGAAG 
GCTCAAAGTA ACGGACCTGA AAAGCAGGAA AAAGAAGGCG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AGAAAGAGGC GATGAGACAA 
GCATCTGATA AAGATGATAG ACCCTTCCAC GGGGAGGACA CTGAGAATTC TCGACTGGCT 
GCCGAGGTTT ACAAAGACAT GCCCGAAACT AGCTTTACCC GAACAATCTC CAACCCCGAG 
GTGGTCATGA AGCGAAGGCG GCAGCAAAAA CTGGAAAAGA GGATGCAGGA GTTTCGGAGC 
TCAGACGGGC GCCCTGATTC CGGTGGAACG TTGAGAATTT ATGCGGATAG TTTAAAGCCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGACC CCGCCGACTT TGCCGTGGCG 
GAGGCTTTGG AGAAGTACGG TCTGGAGAAG GAGAACCCGA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCG GATGAGAAAG GTGCTAAAGA AATTATCCTG 
GACGATGACG AGTGTCCTTT ACAGATCTTC AGGGAATGGC CTGGTGACAA AGGGATGCTG 
GTCTTCCAGC TGAAGAGGAG GCCCCCAGAG CACATCCCCA AGAAGAGCAA GAAGCACGCG 
GAGGGGAAGC CGCCCAAGGG GAAGGAGCGG CCCGACGGCT CTGGCTATGG CTCTGCGCTC 
CCCCCGGAGA AGCTGCCCTA CCTAGTGGAG CTGAGCCCAG GGAGAAGGGG TCACTTTGCC 
TACTACAGCT ATCACCCTCA CGAAGACGGC TCCGACTCCA GAGATAAGCC AAAGCTTTAC 
CGCCTCCAGT TAAGCGTCAC CGAGGTCGGG ACGGAGAAGT TCGATGACAG CTCGATCCAG 
TTGTTTGGTT CCGGAATCCA GCCCCACCAC TGCGACCTCA CCAACATGGA CGGGGTGGTC 
ACTGTCACGC CGAGAAGCGT GGATGCTGAG ACCTACGTGG AAGGACAGCG CATCTCGGAG 
ACGACCATGC TGCAGAGCGG GGTGAAGGTG CAGTTCGGGG CGTCCCACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCGCTCGCC AAGAGGCCGG GGGACGGAGG CCTGGCCGTC 
AAGGGTCCCA GACACAAACC GGGAATCGTT CAGGAGACAG CTTTCGATTT AGGAGGAGAC 
GTTCACGGGG GGACGGCCGT AGCGAGCAGT AAGGGCTCCG CCCGACCGGA CGGCGACAGG 
GCCCCGTCTG CCTCCAGCAG CGCCGAGCGC GGGATGGTGA AGCCGATGGT GCGAATGGAG 
CCGCCGCAGG AGCCCCGCAG GCAGGACACC AGACCGCAGG ACGCCGCTGG GCCTGAGCTG 
ACGCTGCCCG CGAGCATCGA ATTCAGGGAA AGTGCTGAAG ACTCGTTCTT ATCCGCCATC 
ATCAATTACA CGAATAGCTC GACGGTGCAT TTTAAGTTGT CGCCTACGTA CGTGCTGTAC 
ATGGCGTGTC GGCACGTGCT GTCCAGCCAG TACCGCCCGG ACGTCAGCCC CGCCGAGCGC 
ACGCACAAGG TCATCGCCAT CGTCAACAAG ATGGTGAGCA TGATGGACGG CGTCATCCAG 
GAAGTAGACC AGGTGGCTGA GAAACAGAAG AACATCGCGG GGGCGCTTGC GTTCTGGATG 
GCCAACGCGT CGGAACTCCT CAACTTCATC AAGCAGGACC GAGACCTCAG TCGAATCACA 
CTGGACGCCC AGGATGTTTT GGCTCACCTG GTTCAGATGG CCTTTAAATA CCTGGTTCAC 
TGTCTTCAGT CAGAGCTGAA CAACTACATG CCGGCCTTTC TGGACGACCC CGAGGAGAAC 
AGTCTGCAAA GACCAAAAAT AGAGGACGTC CTCCACACGC TCACGGGCGC CATGTCGCTG 
CTGCGGCGCT GCCGGGTCAA CGCCGCCCTC ACCATCCAGC TCTTCTCCCA GCTCTTCCAC 
TTCATCAACA TGTGGCTGTT CAACAGGCTG CTGACCGACC CCGAGTCGGG CCTGTGCTCC 
CACTACTGGG GCGCCATCGT CCGCCAGCAG CTGGGCCACG TGGAGGCCTG GGCGGAGAAG 
CAGGGCCTGG AGCTGGCTGC CGACTGCCAC CTCAGCAGGG TCGTGCAGGC GACCACCCTG 
CTCACGATGG ACAAGTACGC CCCGGAGGAC GCGGCCGGCA TCAACAGCAC CTGCTTCAAG 
CTGAACTCGC TGCAGCTGCA GGCCTTGCTG CACAACTACC ACTGTGCGCC CGACGAGCCC 
CTGGTCCCGC CGGATCTGAT AGAAAGCGTG GTGGCCGTGG CTGAAAACAC GGCGGACGAG 
CTGGCGCGCA GCGACGGCAG GGAGGTGCGG CTGGAGGAGG ACCCTGACCT GCAGCTGCCC 
TTCCTGCTGC CGGAGGACGG CTACTCGTGT GACGTCGTCA GAAACATCCC CAACGGCTTG 
CAGGAGTTTT TGGATCCTTT GTGCCAGAGA GGGTTTTGCA GGCTGACGCC CCAGCCGCGC 
TCTCCGGGTT CCTGGACGAT ACATTTTGAA GGTGCGGAGT ACGAGAGCCG CCTCGCACGG 
GAGAGCACGG AGCTGGCTCA GCCTCTGAGG AAAGAACCCG AAGTCATCAC TGTGACCCTG 
AAGAAGCAGA ACGGGATGGG CCTCAGCATC GTCGCAGCGA AGGGCGCAGG TCAAGACAAA 
CTCGGGATCT ACGTCAAGTC GGTGGTGAGA GGAGGAGCTG CAGACGTGGA CGGGCGGCTG 
GCGGCGGGGG ACCAGCTCCT CAGTGTGGAC GGACGAAGCC TGGTGGGCCT CTCTCAGGAA 
AGGGCAGCAG AGCTGATGAC AAGGACAAGT TCCGTGGTGA CCCTGGAGGT AGCGAAGCAG 
GGAGCCATTT ACCACGGCCT CGCCACCCTG CTCAACCAGC CCTCGCCCAT GATGCAGAGA 
ATTTCAGACC GGCGTGGATC AGGTAAACCC CGACCAAAGA GCGAAGGTTT TGAGCTCTAT 
AATAACTCAG CTCAGAACGG GTCCCCCGAG AGCCCCCCGC TGCCCTGGGC CGAGTACAGC 
GAGCCAAAGA AGCTGCCTGG CGACGAGAGG CTGACGAAGA ACCGCGCCGA CCACCGCTCC 
AGCCCCAACG TTGCAAATCA GCCTCCTAGT CCTGGTGGGA AAGGTGCACA TGCCGCTGGA 
ACAGCAGCCA AGATCACATC CGTCTCCACT GGCAACCTCT GCACCGAGGA GCAGACGCCC 
CCGCCGGGGA CGGAGGCCTA CCCCATCCCC ACGCAGGCCT ACCCCCGAGA GTACTTCACC 
TTCCCGGCCT CCAGGTCCCA GGACCGCGTG GTCCCCCCCC AGAGCCAGTG GCCAAGCTAC 
GAGGAGAAGC CGCACGCGGA CAGCAGCCAC TCCAGCCTCG CGGTTCAGCG GGTGACCCGC 
TCCCAGGAGG AGCTGCGGGA GGACGCGGGG GCCTACCCAC TGCACAGGAT GGAGGCGGTG 
ATGGACCGCA AGTCTGACAG CGACATGTGG GTCGCCCAGA GCTCCTCGAT GGGCTCCAGC 
GCGTCCAGCC AGGAGCACCT GGACCCCTCT TCCAAGGCGG GCACCCCCGC GTCCACCCTC 
ACCAAGAGCG GCCCGGGGCG CTGGAAGACC CCTGCAGTTC TGCAGCCCGC GCCCGGCCCC 
CAGCCGCTCC GGACCGACCT GCCCACGCAC TACGCCGGCG ACGTGGACGG CGTCCCCGTG 
GACCTGCCCC TCCCGCCGCC TCCGGCTGCC GCGGGCCAGG GACTCGCGGG GGGCCAAGGG 
GCCGCCGAGC GGAAGAAGCG GGAGGAGCAG CAGCGCTGGT ACGAGAAGGA GAAGGCGCGC 
CTGGAGGAGG AGCGTGAGCG CAAGCGGCGC GAGCAGGAGC GCAAGCTGGG CCAGATGCGC 
TCGCAGGCGC TGCTCCCCGC CCCGCTCTCC CCCGGCGCCA CCAAGCCCGC CGCCCCGGGG 
CCCCAGGACA CCGTCATCCG GGAGCTGCAG CCTCAGCAGC AGCCGCGCAC CATCGAGCGC 
AGGGACCTGC AGTACATCAC CGTCAGCCGG GAGGAGCTCT CGGCCGGCGA CAGCCTGTCC 
CCCGACCCCT GGAAGCGCGA CGCGCGGGAG AAGCTGGAGA AGCAGCAGCA GATGCACGTG 
GTGGACCTGC TGAGCCGGGA GATTCACGAG CTGCAGAGCA AGGCCGAGCG CTCGGCGGAG 
GAGAGCGGCC GCCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG 
TCCAAGCAGA AGGACGAGGA CGACGACGAG GAGGAGGACG ACGACGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC CGAGCGCAGA GCGCGGCAGA CTGCTGTGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG CGGCGGCAGC AGCAGCTGGA GGAGATGCGG 
CAGCGGGAGG CGGAGGAGCG TGCCCGCCAC GAGGAGGAGC GGCGGCGCCA GGAGGAGGAG 
CGGCTGAAGC GCGAGGCCGA GGAAAAGAGG CGCCAGGAGG AGGGGTACTA CAGCCGCCTG 
GAGGCCGAGC GGCGCAGACA GCACGAGGAG GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG 
CCGGGCCTGG GCCGCCCCCC GCTCCCGCGG GGCTACGAGC CCCCCGGCCC CGCGCCGCCG 
CCCCCGCCCC AGCGCGCCGC CTCCTACCTG CAGGCCCAGG TCCTGTCCCC CGACTCCCTG 
TACACCGCCA AGTTCGTGGC TTACGACGAC GATGACGAGG AGGAGGACGG CCCTGCAGGT 
CAGGATAAGT ACGCCCGCGC CAGGGGGGCC CGCGGGGGCC CCCGCCCGGC CTCCGATGGC 
ATCTTTCTCG GCAGCTCCGC CCAGGCGCCC TGGGCCAGGG CCAACAGTCC TGCGCACAGG 
GCAGGCGCGC CCCCGGCCCC GCCGCCCAAG CCCGGCCCCG CCCGAGGCCG AGGGCCCGCC 
TCGTACATGG GATCTACGGG GACGCTGATG GGCGCCCACG ATGGCTCTCG GGACCCAAGA 
GAGACGCTGT CCAAAAGCCA GGACGCGGAC GTGCCGGGCG GGCCTGAGAG CCTGACCTTC 
CGGGAGCGCC AGCGCCTCTT CTCCCAGGGC CACGAGGTCT CCAGTAAGGT GAAAGCCTCC 
CGCCGGCTGA CGGAGCTGGA GAACGAGCTG AACACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984278.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839732.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OSe229409
Clone ID Related Accession (Same CDS sequence)	XM_013984278.2
Accession Version	XM_013984278.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013984278.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGAAACAG 
AAGAACATCG CGGGGGCGCT TGCGTTCTGG ATGGCCAACG CGTCGGAACT CCTCAACTTC 
ATCAAGCAGG ACCGAGACCT CAGTCGAATC ACACTGGACG CCCAGGATGT TTTGGCTCAC 
CTGGTTCAGA TGGCCTTTAA ATACCTGGTT CACTGTCTTC AGTCAGAGCT GAACAACTAC 
ATGCCGGCCT TTCTGGACGA CCCCGAGGAG AACAGTCTGC AAAGACCAAA AATAGAGGAC 
GTCCTCCACA CGCTCACGGG CGCCATGTCG CTGCTGCGGC GCTGCCGGGT CAACGCCGCC 
CTCACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCATCA ACATGTGGCT GTTCAACAGG 
CTGCTGACCG ACCCCGAGTC GGGCCTGTGC TCCCACTACT GGGGCGCCAT CGTCCGCCAG 
CAGCTGGGCC ACGTGGAGGC CTGGGCGGAG AAGCAGGGCC TGGAGCTGGC TGCCGACTGC 
CACCTCAGCA GGGTCGTGCA GGCGACCACC CTGCTCACGA TGGACAAGTA CGCCCCGGAG 
GACGCGGCCG GCATCAACAG CACCTGCTTC AAGCTGAACT CGCTGCAGCT GCAGGCCTTG 
CTGCACAACT ACCACTGTGC GCCCGACGAG CCCCTGGTCC CGCCGGATCT GATAGAAAGC 
GTGGTGGCCG TGGCTGAAAA CACGGCGGAC GAGCTGGCGC GCAGCGACGG CAGGGAGGTG 
CGGCTGGAGG AGGACCCTGA CCTGCAGCTG CCCTTCCTGC TGCCGGAGGA CGGCTACTCG 
TGTGACGTCG TCAGAAACAT CCCCAACGGC TTGCAGGAGT TTTTGGATCC TTTGTGCCAG 
AGAGGGTTTT GCAGGCTGAC GCCCCAGCCG CGCTCTCCGG GTTCCTGGAC GATACATTTT 
GAAGGTGCGG AGTACGAGAG CCGCCTCGCA CGGGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CCGAAGTCAT CACTGTGACC CTGAAGAAGC AGAACGGGAT GGGCCTCAGC 
ATCGTCGCAG CGAAGGGCGC AGGTCAAGAC AAACTCGGGA TCTACGTCAA GTCGGTGGTG 
AGAGGAGGAG CTGCAGACGT GGACGGGCGG CTGGCGGCGG GGGACCAGCT CCTCAGTGTG 
GACGGACGAA GCCTGGTGGG CCTCTCTCAG GAAAGGGCAG CAGAGCTGAT GACAAGGACA 
AGTTCCGTGG TGACCCTGGA GGTAGCGAAG CAGGGAGCCA TTTACCACGG CCTCGCCACC 
CTGCTCAACC AGCCCTCGCC CATGATGCAG AGAATTTCAG ACCGGCGTGG ATCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTTGAGCTC TATAATAACT CAGCTCAGAA CGGGTCCCCC 
GAGAGCCCCC CGCTGCCCTG GGCCGAGTAC AGCGAGCCAA AGAAGCTGCC TGGCGACGAG 
AGGCTGACGA AGAACCGCGC CGACCACCGC TCCAGCCCCA ACGTTGCAAA TCAGCCTCCT 
AGTCCTGGTG GGAAAGGTGC ACATGCCGCT GGAACAGCAG CCAAGATCAC ATCCGTCTCC 
ACTGGCAACC TCTGCACCGA GGAGCAGACG CCCCCGCCGG GGACGGAGGC CTACCCCATC 
CCCACGCAGG CCTACCCCCG AGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGTCCCCC CCCAGAGCCA GTGGCCAAGC TACGAGGAGA AGCCGCACGC GGACAGCAGC 
CACTCCAGCC TCGCGGTTCA GCGGGTGACC CGCTCCCAGG AGGAGCTGCG GGAGGACGCG 
GGGGCCTACC CACTGCACAG GATGGAGGCG GTGATGGACC GCAAGTCTGA CAGCGACATG 
TGGGTCGCCC AGAGCTCCTC GATGGGCTCC AGCGCGTCCA GCCAGGAGCA CCTGGACCCC 
TCTTCCAAGG CGGGCACCCC CGCGTCCACC CTCACCAAGA GCGGCCCGGG GCGCTGGAAG 
ACCCCTGCAG TTCTGCAGCC CGCGCCCGGC CCCCAGCCGC TCCGGACCGA CCTGCCCACG 
CACTACGCCG GCGACGTGGA CGGCGTCCCC GTGGACCTGC CCCTCCCGCC GCCTCCGGCT 
GCCGCGGGCC AGGGACTCGC GGGGGGCCAA GGGGCCGCCG AGCGGAAGAA GCGGGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAGAAGGCG CGCCTGGAGG AGGAGCGTGA GCGCAAGCGG 
CGCGAGCAGG AGCGCAAGCT GGGCCAGATG CGCTCGCAGG CGCTGCTCCC CGCCCCGCTC 
TCCCCCGGCG CCACCAAGCC CGCCGCCCCG GGGCCCCAGG ACACCGTCAT CCGGGAGCTG 
CAGCCTCAGC AGCAGCCGCG CACCATCGAG CGCAGGGACC TGCAGTACAT CACCGTCAGC 
CGGGAGGAGC TCTCGGCCGG CGACAGCCTG TCCCCCGACC CCTGGAAGCG CGACGCGCGG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC GTGGTGGACC TGCTGAGCCG GGAGATTCAC 
GAGCTGCAGA GCAAGGCCGA GCGCTCGGCG GAGGAGAGCG GCCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAC 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTGT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGACGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGCAGCGGG AGGCGGAGGA GCGTGCCCGC 
CACGAGGAGG AGCGGCGGCG CCAGGAGGAG GAGCGGCTGA AGCGCGAGGC CGAGGAAAAG 
AGGCGCCAGG AGGAGGGGTA CTACAGCCGC CTGGAGGCCG AGCGGCGCAG ACAGCACGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC CCCGCTCCCG 
CGGGGCTACG AGCCCCCCGG CCCCGCGCCG CCGCCCCCGC CCCAGCGCGC CGCCTCCTAC 
CTGCAGGCCC AGGTCCTGTC CCCCGACTCC CTGTACACCG CCAAGTTCGT GGCTTACGAC 
GACGATGACG AGGAGGAGGA CGGCCCTGCA GGTCAGGATA AGTACGCCCG CGCCAGGGGG 
GCCCGCGGGG GCCCCCGCCC GGCCTCCGAT GGCATCTTTC TCGGCAGCTC CGCCCAGGCG 
CCCTGGGCCA GGGCCAACAG TCCTGCGCAC AGGGCAGGCG CGCCCCCGGC CCCGCCGCCC 
AAGCCCGGCC CCGCCCGAGG CCGAGGGCCC GCCTCGTACA TGGGATCTAC GGGGACGCTG 
ATGGGCGCCC ACGATGGCTC TCGGGACCCA AGAGAGACGC TGTCCAAAAG CCAGGACGCG 
GACGTGCCGG GCGGGCCTGA GAGCCTGACC TTCCGGGAGC GCCAGCGCCT CTTCTCCCAG 
GGCCACGAGG TCTCCAGTAA GGTGAAAGCC TCCCGCCGGC TGACGGAGCT GGAGAACGAG 
CTGAACACCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839732.1
CDS	22..5496
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984278.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984278.2

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGAAACAG 
AAGAACATCG CGGGGGCGCT TGCGTTCTGG ATGGCCAACG CGTCGGAACT CCTCAACTTC 
ATCAAGCAGG ACCGAGACCT CAGTCGAATC ACACTGGACG CCCAGGATGT TTTGGCTCAC 
CTGGTTCAGA TGGCCTTTAA ATACCTGGTT CACTGTCTTC AGTCAGAGCT GAACAACTAC 
ATGCCGGCCT TTCTGGACGA CCCCGAGGAG AACAGTCTGC AAAGACCAAA AATAGAGGAC 
GTCCTCCACA CGCTCACGGG CGCCATGTCG CTGCTGCGGC GCTGCCGGGT CAACGCCGCC 
CTCACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCATCA ACATGTGGCT GTTCAACAGG 
CTGCTGACCG ACCCCGAGTC GGGCCTGTGC TCCCACTACT GGGGCGCCAT CGTCCGCCAG 
CAGCTGGGCC ACGTGGAGGC CTGGGCGGAG AAGCAGGGCC TGGAGCTGGC TGCCGACTGC 
CACCTCAGCA GGGTCGTGCA GGCGACCACC CTGCTCACGA TGGACAAGTA CGCCCCGGAG 
GACGCGGCCG GCATCAACAG CACCTGCTTC AAGCTGAACT CGCTGCAGCT GCAGGCCTTG 
CTGCACAACT ACCACTGTGC GCCCGACGAG CCCCTGGTCC CGCCGGATCT GATAGAAAGC 
GTGGTGGCCG TGGCTGAAAA CACGGCGGAC GAGCTGGCGC GCAGCGACGG CAGGGAGGTG 
CGGCTGGAGG AGGACCCTGA CCTGCAGCTG CCCTTCCTGC TGCCGGAGGA CGGCTACTCG 
TGTGACGTCG TCAGAAACAT CCCCAACGGC TTGCAGGAGT TTTTGGATCC TTTGTGCCAG 
AGAGGGTTTT GCAGGCTGAC GCCCCAGCCG CGCTCTCCGG GTTCCTGGAC GATACATTTT 
GAAGGTGCGG AGTACGAGAG CCGCCTCGCA CGGGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CCGAAGTCAT CACTGTGACC CTGAAGAAGC AGAACGGGAT GGGCCTCAGC 
ATCGTCGCAG CGAAGGGCGC AGGTCAAGAC AAACTCGGGA TCTACGTCAA GTCGGTGGTG 
AGAGGAGGAG CTGCAGACGT GGACGGGCGG CTGGCGGCGG GGGACCAGCT CCTCAGTGTG 
GACGGACGAA GCCTGGTGGG CCTCTCTCAG GAAAGGGCAG CAGAGCTGAT GACAAGGACA 
AGTTCCGTGG TGACCCTGGA GGTAGCGAAG CAGGGAGCCA TTTACCACGG CCTCGCCACC 
CTGCTCAACC AGCCCTCGCC CATGATGCAG AGAATTTCAG ACCGGCGTGG ATCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTTGAGCTC TATAATAACT CAGCTCAGAA CGGGTCCCCC 
GAGAGCCCCC CGCTGCCCTG GGCCGAGTAC AGCGAGCCAA AGAAGCTGCC TGGCGACGAG 
AGGCTGACGA AGAACCGCGC CGACCACCGC TCCAGCCCCA ACGTTGCAAA TCAGCCTCCT 
AGTCCTGGTG GGAAAGGTGC ACATGCCGCT GGAACAGCAG CCAAGATCAC ATCCGTCTCC 
ACTGGCAACC TCTGCACCGA GGAGCAGACG CCCCCGCCGG GGACGGAGGC CTACCCCATC 
CCCACGCAGG CCTACCCCCG AGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGTCCCCC CCCAGAGCCA GTGGCCAAGC TACGAGGAGA AGCCGCACGC GGACAGCAGC 
CACTCCAGCC TCGCGGTTCA GCGGGTGACC CGCTCCCAGG AGGAGCTGCG GGAGGACGCG 
GGGGCCTACC CACTGCACAG GATGGAGGCG GTGATGGACC GCAAGTCTGA CAGCGACATG 
TGGGTCGCCC AGAGCTCCTC GATGGGCTCC AGCGCGTCCA GCCAGGAGCA CCTGGACCCC 
TCTTCCAAGG CGGGCACCCC CGCGTCCACC CTCACCAAGA GCGGCCCGGG GCGCTGGAAG 
ACCCCTGCAG TTCTGCAGCC CGCGCCCGGC CCCCAGCCGC TCCGGACCGA CCTGCCCACG 
CACTACGCCG GCGACGTGGA CGGCGTCCCC GTGGACCTGC CCCTCCCGCC GCCTCCGGCT 
GCCGCGGGCC AGGGACTCGC GGGGGGCCAA GGGGCCGCCG AGCGGAAGAA GCGGGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAGAAGGCG CGCCTGGAGG AGGAGCGTGA GCGCAAGCGG 
CGCGAGCAGG AGCGCAAGCT GGGCCAGATG CGCTCGCAGG CGCTGCTCCC CGCCCCGCTC 
TCCCCCGGCG CCACCAAGCC CGCCGCCCCG GGGCCCCAGG ACACCGTCAT CCGGGAGCTG 
CAGCCTCAGC AGCAGCCGCG CACCATCGAG CGCAGGGACC TGCAGTACAT CACCGTCAGC 
CGGGAGGAGC TCTCGGCCGG CGACAGCCTG TCCCCCGACC CCTGGAAGCG CGACGCGCGG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC GTGGTGGACC TGCTGAGCCG GGAGATTCAC 
GAGCTGCAGA GCAAGGCCGA GCGCTCGGCG GAGGAGAGCG GCCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAC 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTGT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGACGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGCAGCGGG AGGCGGAGGA GCGTGCCCGC 
CACGAGGAGG AGCGGCGGCG CCAGGAGGAG GAGCGGCTGA AGCGCGAGGC CGAGGAAAAG 
AGGCGCCAGG AGGAGGGGTA CTACAGCCGC CTGGAGGCCG AGCGGCGCAG ACAGCACGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC CCCGCTCCCG 
CGGGGCTACG AGCCCCCCGG CCCCGCGCCG CCGCCCCCGC CCCAGCGCGC CGCCTCCTAC 
CTGCAGGCCC AGGTCCTGTC CCCCGACTCC CTGTACACCG CCAAGTTCGT GGCTTACGAC 
GACGATGACG AGGAGGAGGA CGGCCCTGCA GGTCAGGATA AGTACGCCCG CGCCAGGGGG 
GCCCGCGGGG GCCCCCGCCC GGCCTCCGAT GGCATCTTTC TCGGCAGCTC CGCCCAGGCG 
CCCTGGGCCA GGGCCAACAG TCCTGCGCAC AGGGCAGGCG CGCCCCCGGC CCCGCCGCCC 
AAGCCCGGCC CCGCCCGAGG CCGAGGGCCC GCCTCGTACA TGGGATCTAC GGGGACGCTG 
ATGGGCGCCC ACGATGGCTC TCGGGACCCA AGAGAGACGC TGTCCAAAAG CCAGGACGCG 
GACGTGCCGG GCGGGCCTGA GAGCCTGACC TTCCGGGAGC GCCAGCGCCT CTTCTCCCAG 
GGCCACGAGG TCTCCAGTAA GGTGAAAGCC TCCCGCCGGC TGACGGAGCT GGAGAACGAG 
CTGAACACCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984285.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839739.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

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CloneID	OSe229410
Clone ID Related Accession (Same CDS sequence)	XM_013984285.2
Accession Version	XM_013984285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013984285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGACGCGGCC GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG TTGCAGGACG AGGAGCGGCG GCGGCAGCAG 
CAGCTGGAGG AGATGCGGCA GCGGGAGGCG GAGGAGCGTG CCCGCCACGA GGAGGAGCGG 
CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC GAGGCCGAGG AAAAGAGGCG CCAGGAGGAG 
GGGTACTACA GCCGCCTGGA GGCCGAGCGG CGCAGACAGC ACGAGGAGGC CGAGCGCCGG 
CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC CGCCCCCCGC TCCCGCGGGG CTACGAGCCC 
CCCGGCCCCG CGCCGCCGCC CCCGCCCCAG CGCGCCGCCT CCTACCTGCA GGCCCAGGTC 
CTGTCCCCCG ACTCCCTGTA CACCGCCAAG TTCGTGGCTT ACGACGACGA TGACGAGGAG 
GAGGACGGCC CTGCAGGTCA GGATAAGTAC GCCCGCGCCA GGGGGGCCCG CGGGGGCCCC 
CGCCCGGCCT CCGATGGCAT CTTTCTCGGC AGCTCCGCCC AGGCGCCCTG GGCCAGGGCC 
AACAGTCCTG CGCACAGGGC AGGCGCGCCC CCGGCCCCGC CGCCCAAGCC CGGCCCCGCC 
CGAGGCCGAG GGCCCGCCTC GTACATGGGA TCTACGGGGA CGCTGATGGG CGCCCACGAT 
GGCTCTCGGG ACCCAAGAGA GACGCTGTCC AAAAGCCAGG ACGCGGACGT GCCGGGCGGG 
CCTGAGAGCC TGACCTTCCG GGAGCGCCAG CGCCTCTTCT CCCAGGGCCA CGAGGTCTCC 
AGTAAGGTGA AAGCCTCCCG CCGGCTGACG GAGCTGGAGA ACGAGCTGAA CACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839739.1
CDS	22..5481
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984285.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984285.2

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGACGCGGCC GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG TTGCAGGACG AGGAGCGGCG GCGGCAGCAG 
CAGCTGGAGG AGATGCGGCA GCGGGAGGCG GAGGAGCGTG CCCGCCACGA GGAGGAGCGG 
CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC GAGGCCGAGG AAAAGAGGCG CCAGGAGGAG 
GGGTACTACA GCCGCCTGGA GGCCGAGCGG CGCAGACAGC ACGAGGAGGC CGAGCGCCGG 
CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC CGCCCCCCGC TCCCGCGGGG CTACGAGCCC 
CCCGGCCCCG CGCCGCCGCC CCCGCCCCAG CGCGCCGCCT CCTACCTGCA GGCCCAGGTC 
CTGTCCCCCG ACTCCCTGTA CACCGCCAAG TTCGTGGCTT ACGACGACGA TGACGAGGAG 
GAGGACGGCC CTGCAGGTCA GGATAAGTAC GCCCGCGCCA GGGGGGCCCG CGGGGGCCCC 
CGCCCGGCCT CCGATGGCAT CTTTCTCGGC AGCTCCGCCC AGGCGCCCTG GGCCAGGGCC 
AACAGTCCTG CGCACAGGGC AGGCGCGCCC CCGGCCCCGC CGCCCAAGCC CGGCCCCGCC 
CGAGGCCGAG GGCCCGCCTC GTACATGGGA TCTACGGGGA CGCTGATGGG CGCCCACGAT 
GGCTCTCGGG ACCCAAGAGA GACGCTGTCC AAAAGCCAGG ACGCGGACGT GCCGGGCGGG 
CCTGAGAGCC TGACCTTCCG GGAGCGCCAG CGCCTCTTCT CCCAGGGCCA CGAGGTCTCC 
AGTAAGGTGA AAGCCTCCCG CCGGCTGACG GAGCTGGAGA ACGAGCTGAA CACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_021085803.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020941462.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

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CloneID	OSe229411
Clone ID Related Accession (Same CDS sequence)	XM_021085803.1
Accession Version	XM_021085803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA CGAGAAGCCC CTAGTTGTGC AGCTGAATTG GAATAAAGAT 
GACCGCGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGACG CCGCTCCTCC CAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAGA AAGAGGCGAT GAGACAAGCA 
TCTGATAAAG ATGATAGACC CTTCCACGGG GAGGACACTG AGAATTCTCG ACTGGCTGCC 
GAGGTTTACA AAGACATGCC CGAAACTAGC TTTACCCGAA CAATCTCCAA CCCCGAGGTG 
GTCATGAAGC GAAGGCGGCA GCAAAAACTG GAAAAGAGGA TGCAGGAGTT TCGGAGCTCA 
GACGGGCGCC CTGATTCCGG TGGAACGTTG AGAATTTATG CGGATAGTTT AAAGCCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGACCCCG CCGACTTTGC CGTGGCGGAG 
GCTTTGGAGA AGTACGGTCT GGAGAAGGAG AACCCGAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAGAAAGGTG CTAAAGAAAT TATCCTGGAC 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAATGGCCTG GTGACAAAGG GATGCTGGTC 
TTCCAGCTGA AGAGGAGGCC CCCAGAGCAC ATCCCCAAGA AGAGCAAGAA GCACGCGGAG 
GGGAAGCCGC CCAAGGGGAA GGAGCGGCCC GACGGCTCTG GCTATGGCTC TGCGCTCCCC 
CCGGAGAAGC TGCCCTACCT AGTGGAGCTG AGCCCAGGGA GAAGGGGTCA CTTTGCCTAC 
TACAGCTATC ACCCTCACGA AGACGGCTCC GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTCCAGTTAA GCGTCACCGA GGTCGGGACG GAGAAGTTCG ATGACAGCTC GATCCAGTTG 
TTTGGTTCCG GAATCCAGCC CCACCACTGC GACCTCACCA ACATGGACGG GGTGGTCACT 
GTCACGCCGA GAAGCGTGGA TGCTGAGACC TACGTGGAAG GACAGCGCAT CTCGGAGACG 
ACCATGCTGC AGAGCGGGGT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC GCTCGCCAAG AGGCCGGGGG ACGGAGGCCT GGCCGTCAAG 
GGTCCCAGAC ACAAACCGGG AATCGTTCAG GAGACAGCTT TCGATTTAGG AGGAGACGTT 
CACGGGGGGA CGGCCGTAGC GAGCAGTAAG GGCTCCGCCC GACCGGACGG CGACAGGGCC 
CCGTCTGCCT CCAGCAGCGC CGAGCGCGGG ATGGTGAAGC CGATGGTGCG AATGGAGCCG 
CCGCAGGAGC CCCGCAGGCA GGACACCAGA CCGCAGGACG CCGCTGGGCC TGAGCTGACG 
CTGCCCGCGA GCATCGAATT CAGGGAAAGT GCTGAAGACT CGTTCTTATC CGCCATCATC 
AATTACACGA ATAGCTCGAC GGTGCATTTT AAGTTGTCGC CTACGTACGT GCTGTACATG 
GCGTGTCGGC ACGTGCTGTC CAGCCAGTAC CGCCCGGACG TCAGCCCCGC CGAGCGCACG 
CACAAGGTCA TCGCCATCGT CAACAAGATG GTGAGCATGA TGGACGGCGT CATCCAGAAA 
CAGAAGAACA TCGCGGGGGC GCTTGCGTTC TGGATGGCCA ACGCGTCGGA ACTCCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGA ATCACACTGG ACGCCCAGGA TGTTTTGGCT 
CACCTGGTTC AGATGGCCTT TAAATACCTG GTTCACTGTC TTCAGTCAGA GCTGAACAAC 
TACATGCCGG CCTTTCTGGA CGACCCCGAG GAGAACAGTC TGCAAAGACC AAAAATAGAG 
GACGTCCTCC ACACGCTCAC GGGCGCCATG TCGCTGCTGC GGCGCTGCCG GGTCAACGCC 
GCCCTCACCA TCCAGCTCTT CTCCCAGCTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
AGGCTGCTGA CCGACCCCGA GTCGGGCCTG TGCTCCCACT ACTGGGGCGC CATCGTCCGC 
CAGCAGCTGG GCCACGTGGA GGCCTGGGCG GAGAAGCAGG GCCTGGAGCT GGCTGCCGAC 
TGCCACCTCA GCAGGGTCGT GCAGGCGACC ACCCTGCTCA CGATGGACAA GTACGCCCCG 
GAGGACGCGG CCGGCATCAA CAGCACCTGC TTCAAGCTGA ACTCGCTGCA GCTGCAGGCC 
TTGCTGCACA ACTACCACTG TGCGCCCGAC GAGCCCCTGG TCCCGCCGGA TCTGATAGAA 
AGCGTGGTGG CCGTGGCTGA AAACACGGCG GACGAGCTGG CGCGCAGCGA CGGCAGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TGCTGCCGGA GGACGGCTAC 
TCGTGTGACG TCGTCAGAAA CATCCCCAAC GGCTTGCAGG AGTTTTTGGA TCCTTTGTGC 
CAGAGAGGGT TTTGCAGGCT GACGCCCCAG CCGCGCTCTC CGGGTTCCTG GACGATACAT 
TTTGAAGGTG CGGAGTACGA GAGCCGCCTC GCACGGGAGA GCACGGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCCGAAGT CATCACTGTG ACCCTGAAGA AGCAGAACGG GATGGGCCTC 
AGCATCGTCG CAGCGAAGGG CGCAGGTCAA GACAAACTCG GGATCTACGT CAAGTCGGTG 
GTGAGAGGAG GAGCTGCAGA CGTGGACGGG CGGCTGGCGG CGGGGGACCA GCTCCTCAGT 
GTGGACGGAC GAAGCCTGGT GGGCCTCTCT CAGGAAAGGG CAGCAGAGCT GATGACAAGG 
ACAAGTTCCG TGGTGACCCT GGAGGTAGCG AAGCAGGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCCTC GCCCATGATG CAGAGAATTT CAGACCGGCG TGGATCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTTGAG CTCTATAATA ACTCAGCTCA GAACGGGTCC 
CCCGAGAGCC CCCCGCTGCC CTGGGCCGAG TACAGCGAGC CAAAGAAGCT GCCTGGCGAC 
GAGAGGCTGA CGAAGAACCG CGCCGACCAC CGCTCCAGCC CCAACGTTGC AAATCAGCCT 
CCTAGTCCTG GTGGGAAAGG TGCACATGCC GCTGGAACAG CAGCCAAGAT CACATCCGTC 
TCCACTGGCA ACCTCTGCAC CGAGGAGCAG ACGCCCCCGC CGGGGACGGA GGCCTACCCC 
ATCCCCACGC AGGCCTACCC CCGAGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGTCC CCCCCCAGAG CCAGTGGCCA AGCTACGAGG AGAAGCCGCA CGCGGACAGC 
AGCCACTCCA GCCTCGCGGT TCAGCGGGTG ACCCGCTCCC AGGAGGAGCT GCGGGAGGAC 
GCGGGGGCCT ACCCACTGCA CAGGATGGAG GCGGTGATGG ACCGCAAGTC TGACAGCGAC 
ATGTGGGTCG CCCAGAGCTC CTCGATGGGC TCCAGCGCGT CCAGCCAGGA GCACCTGGAC 
CCCTCTTCCA AGGCGGGCAC CCCCGCGTCC ACCCTCACCA AGAGCGGCCC GGGGCGCTGG 
AAGACCCCTG CAGTTCTGCA GCCCGCGCCC GGCCCCCAGC CGCTCCGGAC CGACCTGCCC 
ACGCACTACG CCGGCGACGT GGACGGCGTC CCCGTGGACC TGCCCCTCCC GCCGCCTCCG 
GCTGCCGCGG GCCAGGGACT CGCGGGGGGC CAAGGGGCCG CCGAGCGGAA GAAGCGGGAG 
GAGCAGCAGC GCTGGTACGA GAAGGAGAAG GCGCGCCTGG AGGAGGAGCG TGAGCGCAAG 
CGGCGCGAGC AGGAGCGCAA GCTGGGCCAG ATGCGCTCGC AGGCGCTGCT CCCCGCCCCG 
CTCTCCCCCG GCGCCACCAA GCCCGCCGCC CCGGGGCCCC AGGACACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC GCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACCGTC 
AGCCGGGAGG AGCTCTCGGC CGGCGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGACGCG 
CGGGAGAAGC TGGAGAAGCA GCAGCAGATG CACGTGGTGG ACCTGCTGAG CCGGGAGATT 
CACGAGCTGC AGAGCAAGGC CGAGCGCTCG GCGGAGGAGA GCGGCCGCCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GACGAGGAGG AGGACGACGA CGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TGTGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGACGAG 
GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG ATGCGGCAGC GGGAGGCGGA GGAGCGTGCC 
CGCCACGAGG AGGAGCGGCG GCGCCAGGAG GAGGAGCGGC TGAAGCGCGA GGCCGAGGAA 
AAGAGGCGCC AGGAGGAGGG GTACTACAGC CGCCTGGAGG CCGAGCGGCG CAGACAGCAC 
GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC GAGGAGCCGG GCCTGGGCCG CCCCCCGCTC 
CCGCGGGGCT ACGAGCCCCC CGGCCCCGCG CCGCCGCCCC CGCCCCAGCG CGCCGCCTCC 
TACCTGCAGG CCCAGGTCCT GTCCCCCGAC TCCCTGTACA CCGCCAAGTT CGTGGCTTAC 
GACGACGATG ACGAGGAGGA GGACGGCCCT GCAGGTCAGG ATAAGTACGC CCGCGCCAGG 
GGGGCCCGCG GGGGCCCCCG CCCGGCCTCC GATGGCATCT TTCTCGGCAG CTCCGCCCAG 
GCGCCCTGGG CCAGGGCCAA CAGTCCTGCG CACAGGGCAG GCGCGCCCCC GGCCCCGCCG 
CCCAAGCCCG GCCCCGCCCG AGGCCGAGGG CCCGCCTCGT ACATGGGATC TACGGGGACG 
CTGATGGGCG CCCACGATGG CTCTCGGGAC CCAAGAGAGA CGCTGTCCAA AAGCCAGGAC 
GCGGACGTGC CGGGCGGGCC TGAGAGCCTG ACCTTCCGGG AGCGCCAGCG CCTCTTCTCC 
CAGGGCCACG AGGTCTCCAG TAAGGTGAAA GCCTCCCGCC GGCTGACGGA GCTGGAGAAC 
GAGCTGAACA CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020941462.1
CDS	28..5625
Translation	MSAGARDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_021085803.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021085803.1

ORF Insert Sequence:

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA CGAGAAGCCC CTAGTTGTGC AGCTGAATTG GAATAAAGAT 
GACCGCGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGACG CCGCTCCTCC CAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAGA AAGAGGCGAT GAGACAAGCA 
TCTGATAAAG ATGATAGACC CTTCCACGGG GAGGACACTG AGAATTCTCG ACTGGCTGCC 
GAGGTTTACA AAGACATGCC CGAAACTAGC TTTACCCGAA CAATCTCCAA CCCCGAGGTG 
GTCATGAAGC GAAGGCGGCA GCAAAAACTG GAAAAGAGGA TGCAGGAGTT TCGGAGCTCA 
GACGGGCGCC CTGATTCCGG TGGAACGTTG AGAATTTATG CGGATAGTTT AAAGCCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGACCCCG CCGACTTTGC CGTGGCGGAG 
GCTTTGGAGA AGTACGGTCT GGAGAAGGAG AACCCGAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAGAAAGGTG CTAAAGAAAT TATCCTGGAC 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAATGGCCTG GTGACAAAGG GATGCTGGTC 
TTCCAGCTGA AGAGGAGGCC CCCAGAGCAC ATCCCCAAGA AGAGCAAGAA GCACGCGGAG 
GGGAAGCCGC CCAAGGGGAA GGAGCGGCCC GACGGCTCTG GCTATGGCTC TGCGCTCCCC 
CCGGAGAAGC TGCCCTACCT AGTGGAGCTG AGCCCAGGGA GAAGGGGTCA CTTTGCCTAC 
TACAGCTATC ACCCTCACGA AGACGGCTCC GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTCCAGTTAA GCGTCACCGA GGTCGGGACG GAGAAGTTCG ATGACAGCTC GATCCAGTTG 
TTTGGTTCCG GAATCCAGCC CCACCACTGC GACCTCACCA ACATGGACGG GGTGGTCACT 
GTCACGCCGA GAAGCGTGGA TGCTGAGACC TACGTGGAAG GACAGCGCAT CTCGGAGACG 
ACCATGCTGC AGAGCGGGGT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC GCTCGCCAAG AGGCCGGGGG ACGGAGGCCT GGCCGTCAAG 
GGTCCCAGAC ACAAACCGGG AATCGTTCAG GAGACAGCTT TCGATTTAGG AGGAGACGTT 
CACGGGGGGA CGGCCGTAGC GAGCAGTAAG GGCTCCGCCC GACCGGACGG CGACAGGGCC 
CCGTCTGCCT CCAGCAGCGC CGAGCGCGGG ATGGTGAAGC CGATGGTGCG AATGGAGCCG 
CCGCAGGAGC CCCGCAGGCA GGACACCAGA CCGCAGGACG CCGCTGGGCC TGAGCTGACG 
CTGCCCGCGA GCATCGAATT CAGGGAAAGT GCTGAAGACT CGTTCTTATC CGCCATCATC 
AATTACACGA ATAGCTCGAC GGTGCATTTT AAGTTGTCGC CTACGTACGT GCTGTACATG 
GCGTGTCGGC ACGTGCTGTC CAGCCAGTAC CGCCCGGACG TCAGCCCCGC CGAGCGCACG 
CACAAGGTCA TCGCCATCGT CAACAAGATG GTGAGCATGA TGGACGGCGT CATCCAGAAA 
CAGAAGAACA TCGCGGGGGC GCTTGCGTTC TGGATGGCCA ACGCGTCGGA ACTCCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGA ATCACACTGG ACGCCCAGGA TGTTTTGGCT 
CACCTGGTTC AGATGGCCTT TAAATACCTG GTTCACTGTC TTCAGTCAGA GCTGAACAAC 
TACATGCCGG CCTTTCTGGA CGACCCCGAG GAGAACAGTC TGCAAAGACC AAAAATAGAG 
GACGTCCTCC ACACGCTCAC GGGCGCCATG TCGCTGCTGC GGCGCTGCCG GGTCAACGCC 
GCCCTCACCA TCCAGCTCTT CTCCCAGCTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
AGGCTGCTGA CCGACCCCGA GTCGGGCCTG TGCTCCCACT ACTGGGGCGC CATCGTCCGC 
CAGCAGCTGG GCCACGTGGA GGCCTGGGCG GAGAAGCAGG GCCTGGAGCT GGCTGCCGAC 
TGCCACCTCA GCAGGGTCGT GCAGGCGACC ACCCTGCTCA CGATGGACAA GTACGCCCCG 
GAGGACGCGG CCGGCATCAA CAGCACCTGC TTCAAGCTGA ACTCGCTGCA GCTGCAGGCC 
TTGCTGCACA ACTACCACTG TGCGCCCGAC GAGCCCCTGG TCCCGCCGGA TCTGATAGAA 
AGCGTGGTGG CCGTGGCTGA AAACACGGCG GACGAGCTGG CGCGCAGCGA CGGCAGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TGCTGCCGGA GGACGGCTAC 
TCGTGTGACG TCGTCAGAAA CATCCCCAAC GGCTTGCAGG AGTTTTTGGA TCCTTTGTGC 
CAGAGAGGGT TTTGCAGGCT GACGCCCCAG CCGCGCTCTC CGGGTTCCTG GACGATACAT 
TTTGAAGGTG CGGAGTACGA GAGCCGCCTC GCACGGGAGA GCACGGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCCGAAGT CATCACTGTG ACCCTGAAGA AGCAGAACGG GATGGGCCTC 
AGCATCGTCG CAGCGAAGGG CGCAGGTCAA GACAAACTCG GGATCTACGT CAAGTCGGTG 
GTGAGAGGAG GAGCTGCAGA CGTGGACGGG CGGCTGGCGG CGGGGGACCA GCTCCTCAGT 
GTGGACGGAC GAAGCCTGGT GGGCCTCTCT CAGGAAAGGG CAGCAGAGCT GATGACAAGG 
ACAAGTTCCG TGGTGACCCT GGAGGTAGCG AAGCAGGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCCTC GCCCATGATG CAGAGAATTT CAGACCGGCG TGGATCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTTGAG CTCTATAATA ACTCAGCTCA GAACGGGTCC 
CCCGAGAGCC CCCCGCTGCC CTGGGCCGAG TACAGCGAGC CAAAGAAGCT GCCTGGCGAC 
GAGAGGCTGA CGAAGAACCG CGCCGACCAC CGCTCCAGCC CCAACGTTGC AAATCAGCCT 
CCTAGTCCTG GTGGGAAAGG TGCACATGCC GCTGGAACAG CAGCCAAGAT CACATCCGTC 
TCCACTGGCA ACCTCTGCAC CGAGGAGCAG ACGCCCCCGC CGGGGACGGA GGCCTACCCC 
ATCCCCACGC AGGCCTACCC CCGAGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGTCC CCCCCCAGAG CCAGTGGCCA AGCTACGAGG AGAAGCCGCA CGCGGACAGC 
AGCCACTCCA GCCTCGCGGT TCAGCGGGTG ACCCGCTCCC AGGAGGAGCT GCGGGAGGAC 
GCGGGGGCCT ACCCACTGCA CAGGATGGAG GCGGTGATGG ACCGCAAGTC TGACAGCGAC 
ATGTGGGTCG CCCAGAGCTC CTCGATGGGC TCCAGCGCGT CCAGCCAGGA GCACCTGGAC 
CCCTCTTCCA AGGCGGGCAC CCCCGCGTCC ACCCTCACCA AGAGCGGCCC GGGGCGCTGG 
AAGACCCCTG CAGTTCTGCA GCCCGCGCCC GGCCCCCAGC CGCTCCGGAC CGACCTGCCC 
ACGCACTACG CCGGCGACGT GGACGGCGTC CCCGTGGACC TGCCCCTCCC GCCGCCTCCG 
GCTGCCGCGG GCCAGGGACT CGCGGGGGGC CAAGGGGCCG CCGAGCGGAA GAAGCGGGAG 
GAGCAGCAGC GCTGGTACGA GAAGGAGAAG GCGCGCCTGG AGGAGGAGCG TGAGCGCAAG 
CGGCGCGAGC AGGAGCGCAA GCTGGGCCAG ATGCGCTCGC AGGCGCTGCT CCCCGCCCCG 
CTCTCCCCCG GCGCCACCAA GCCCGCCGCC CCGGGGCCCC AGGACACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC GCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACCGTC 
AGCCGGGAGG AGCTCTCGGC CGGCGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGACGCG 
CGGGAGAAGC TGGAGAAGCA GCAGCAGATG CACGTGGTGG ACCTGCTGAG CCGGGAGATT 
CACGAGCTGC AGAGCAAGGC CGAGCGCTCG GCGGAGGAGA GCGGCCGCCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GACGAGGAGG AGGACGACGA CGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGCAGACTGC TGTGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGACGAG 
GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG ATGCGGCAGC GGGAGGCGGA GGAGCGTGCC 
CGCCACGAGG AGGAGCGGCG GCGCCAGGAG GAGGAGCGGC TGAAGCGCGA GGCCGAGGAA 
AAGAGGCGCC AGGAGGAGGG GTACTACAGC CGCCTGGAGG CCGAGCGGCG CAGACAGCAC 
GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC GAGGAGCCGG GCCTGGGCCG CCCCCCGCTC 
CCGCGGGGCT ACGAGCCCCC CGGCCCCGCG CCGCCGCCCC CGCCCCAGCG CGCCGCCTCC 
TACCTGCAGG CCCAGGTCCT GTCCCCCGAC TCCCTGTACA CCGCCAAGTT CGTGGCTTAC 
GACGACGATG ACGAGGAGGA GGACGGCCCT GCAGGTCAGG ATAAGTACGC CCGCGCCAGG 
GGGGCCCGCG GGGGCCCCCG CCCGGCCTCC GATGGCATCT TTCTCGGCAG CTCCGCCCAG 
GCGCCCTGGG CCAGGGCCAA CAGTCCTGCG CACAGGGCAG GCGCGCCCCC GGCCCCGCCG 
CCCAAGCCCG GCCCCGCCCG AGGCCGAGGG CCCGCCTCGT ACATGGGATC TACGGGGACG 
CTGATGGGCG CCCACGATGG CTCTCGGGAC CCAAGAGAGA CGCTGTCCAA AAGCCAGGAC 
GCGGACGTGC CGGGCGGGCC TGAGAGCCTG ACCTTCCGGG AGCGCCAGCG CCTCTTCTCC 
CAGGGCCACG AGGTCTCCAG TAAGGTGAAA GCCTCCCGCC GGCTGACGGA GCTGGAGAAC 
GAGCTGAACA CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984289.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839743.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

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CloneID	OSe229412
Clone ID Related Accession (Same CDS sequence)	XM_013984289.2
Accession Version	XM_013984289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013984289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGACGGCT CCGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTCCAGTT AAGCGTCACC GAGGTCGGGA CGGAGAAGTT CGATGACAGC 
TCGATCCAGT TGTTTGGTTC CGGAATCCAG CCCCACCACT GCGACCTCAC CAACATGGAC 
GGGGTGGTCA CTGTCACGCC GAGAAGCGTG GATGCTGAGA CCTACGTGGA AGGACAGCGC 
ATCTCGGAGA CGACCATGCT GCAGAGCGGG GTGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCGCTCGCCA AGAGGCCGGG GGACGGAGGC 
CTGGCCGTCA AGGGTCCCAG ACACAAACCG GGAATCGTTC AGGAGACAGC TTTCGATTTA 
GGAGGAGACG TTCACGGGGG GACGGCCGTA GCGAGCAGTA AGGGCTCCGC CCGACCGGAC 
GGCGACAGGG CCCCGTCTGC CTCCAGCAGC GCCGAGCGCG GGATGGTGAA GCCGATGGTG 
CGAATGGAGC CGCCGCAGGA GCCCCGCAGG CAGGACACCA GACCGCAGGA CGCCGCTGGG 
CCTGAGCTGA CGCTGCCCGC GAGCATCGAA TTCAGGGAAA GTGCTGAAGA CTCGTTCTTA 
TCCGCCATCA TCAATTACAC GAATAGCTCG ACGGTGCATT TTAAGTTGTC GCCTACGTAC 
GTGCTGTACA TGGCGTGTCG GCACGTGCTG TCCAGCCAGT ACCGCCCGGA CGTCAGCCCC 
GCCGAGCGCA CGCACAAGGT CATCGCCATC GTCAACAAGA TGGTGAGCAT GATGGACGGC 
GTCATCCAGG AAGTAGACCA GGTGGCTGAG AAACAGAAGA ACATCGCGGG GGCGCTTGCG 
TTCTGGATGG CCAACGCGTC GGAACTCCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGAATCACAC TGGACGCCCA GGATGTTTTG GCTCACCTGG TTCAGATGGC CTTTAAATAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAC AACTACATGC CGGCCTTTCT GGACGACCCC 
GAGGAGAACA GTCTGCAAAG ACCAAAAATA GAGGACGTCC TCCACACGCT CACGGGCGCC 
ATGTCGCTGC TGCGGCGCTG CCGGGTCAAC GCCGCCCTCA CCATCCAGCT CTTCTCCCAG 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACAGGCTGC TGACCGACCC CGAGTCGGGC 
CTGTGCTCCC ACTACTGGGG CGCCATCGTC CGCCAGCAGC TGGGCCACGT GGAGGCCTGG 
GCGGAGAAGC AGGGCCTGGA GCTGGCTGCC GACTGCCACC TCAGCAGGGT CGTGCAGGCG 
ACCACCCTGC TCACGATGGA CAAGTACGCC CCGGAGGACG CGGCCGGCAT CAACAGCACC 
TGCTTCAAGC TGAACTCGCT GCAGCTGCAG GCCTTGCTGC ACAACTACCA CTGTGCGCCC 
GACGAGCCCC TGGTCCCGCC GGATCTGATA GAAAGCGTGG TGGCCGTGGC TGAAAACACG 
GCGGACGAGC TGGCGCGCAG CGACGGCAGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCCT TCCTGCTGCC GGAGGACGGC TACTCGTGTG ACGTCGTCAG AAACATCCCC 
AACGGCTTGC AGGAGTTTTT GGATCCTTTG TGCCAGAGAG GGTTTTGCAG GCTGACGCCC 
CAGCCGCGCT CTCCGGGTTC CTGGACGATA CATTTTGAAG GTGCGGAGTA CGAGAGCCGC 
CTCGCACGGG AGAGCACGGA GCTGGCTCAG CCTCTGAGGA AAGAACCCGA AGTCATCACT 
GTGACCCTGA AGAAGCAGAA CGGGATGGGC CTCAGCATCG TCGCAGCGAA GGGCGCAGGT 
CAAGACAAAC TCGGGATCTA CGTCAAGTCG GTGGTGAGAG GAGGAGCTGC AGACGTGGAC 
GGGCGGCTGG CGGCGGGGGA CCAGCTCCTC AGTGTGGACG GACGAAGCCT GGTGGGCCTC 
TCTCAGGAAA GGGCAGCAGA GCTGATGACA AGGACAAGTT CCGTGGTGAC CCTGGAGGTA 
GCGAAGCAGG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC CTCGCCCATG 
ATGCAGAGAA TTTCAGACCG GCGTGGATCA GGTAAACCCC GACCAAAGAG CGAAGGTTTT 
GAGCTCTATA ATAACTCAGC TCAGAACGGG TCCCCCGAGA GCCCCCCGCT GCCCTGGGCC 
GAGTACAGCG AGCCAAAGAA GCTGCCTGGC GACGAGAGGC TGACGAAGAA CCGCGCCGAC 
CACCGCTCCA GCCCCAACGT TGCAAATCAG CCTCCTAGTC CTGGTGGGAA AGGTGCACAT 
GCCGCTGGAA CAGCAGCCAA GATCACATCC GTCTCCACTG GCAACCTCTG CACCGAGGAG 
CAGACGCCCC CGCCGGGGAC GGAGGCCTAC CCCATCCCCA CGCAGGCCTA CCCCCGAGAG 
TACTTCACCT TCCCGGCCTC CAGGTCCCAG GACCGCGTGG TCCCCCCCCA GAGCCAGTGG 
CCAAGCTACG AGGAGAAGCC GCACGCGGAC AGCAGCCACT CCAGCCTCGC GGTTCAGCGG 
GTGACCCGCT CCCAGGAGGA GCTGCGGGAG GACGCGGGGG CCTACCCACT GCACAGGATG 
GAGGCGGTGA TGGACCGCAA GTCTGACAGC GACATGTGGG TCGCCCAGAG CTCCTCGATG 
GGCTCCAGCG CGTCCAGCCA GGAGCACCTG GACCCCTCTT CCAAGGCGGG CACCCCCGCG 
TCCACCCTCA CCAAGAGCGG CCCGGGGCGC TGGAAGACCC CTGCAGTTCT GCAGCCCGCG 
CCCGGCCCCC AGCCGCTCCG GACCGACCTG CCCACGCACT ACGCCGGCGA CGTGGACGGC 
GTCCCCGTGG ACCTGCCCCT CCCGCCGCCT CCGGCTGCCG CGGGCCAGGG ACTCGCGGGG 
GGCCAAGGGG CCGCCGAGCG GAAGAAGCGG GAGGAGCAGC AGCGCTGGTA CGAGAAGGAG 
AAGGCGCGCC TGGAGGAGGA GCGTGAGCGC AAGCGGCGCG AGCAGGAGCG CAAGCTGGGC 
CAGATGCGCT CGCAGGCGCT GCTCCCCGCC CCGCTCTCCC CCGGCGCCAC CAAGCCCGCC 
GCCCCGGGGC CCCAGGACAC CGTCATCCGG GAGCTGCAGC CTCAGCAGCA GCCGCGCACC 
ATCGAGCGCA GGGACCTGCA GTACATCACC GTCAGCCGGG AGGAGCTCTC GGCCGGCGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGCGAC GCGCGGGAGA AGCTGGAGAA GCAGCAGCAG 
ATGCACGTGG TGGACCTGCT GAGCCGGGAG ATTCACGAGC TGCAGAGCAA GGCCGAGCGC 
TCGGCGGAGG AGAGCGGCCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGCGG 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGACGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTGGAGGCC GAGCGCAGAG CGCGGCAGAC TGCTGTGCCA 
GCAATCTCTG TTCTAGATTT GTTGCAGGAC GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG 
GAGATGCGGC AGCGGGAGGC GGAGGAGCGT GCCCGCCACG AGGAGGAGCG GCGGCGCCAG 
GAGGAGGAGC GGCTGAAGCG CGAGGCCGAG GAAAAGAGGC GCCAGGAGGA GGGGTACTAC 
AGCCGCCTGG AGGCCGAGCG GCGCAGACAG CACGAGGAGG CCGAGCGCCG GCTGCTGGAG 
CCCGAGGAGC CGGGCCTGGG CCGCCCCCCG CTCCCGCGGG GCTACGAGCC CCCCGGCCCC 
GCGCCGCCGC CCCCGCCCCA GCGCGCCGCC TCCTACCTGC AGGCCCAGGT CCTGTCCCCC 
GACTCCCTGT ACACCGCCAA GTTCGTGGCT TACGACGACG ATGACGAGGA GGAGGACGGC 
CCTGCAGGTC AGGATAAGTA CGCCCGCGCC AGGGGGGCCC GCGGGGGCCC CCGCCCGGCC 
TCCGATGGCA TCTTTCTCGG CAGCTCCGCC CAGGCGCCCT GGGCCAGGGC CAACAGTCCT 
GCGCACAGGG CAGGCGCGCC CCCGGCCCCG CCGCCCAAGC CCGGCCCCGC CCGAGGCCGA 
GGGCCCGCCT CGTACATGGG ATCTACGGGG ACGCTGATGG GCGCCCACGA TGGCTCTCGG 
GACCCAAGAG AGACGCTGTC CAAAAGCCAG GACGCGGACG TGCCGGGCGG GCCTGAGAGC 
CTGACCTTCC GGGAGCGCCA GCGCCTCTTC TCCCAGGGCC ACGAGGTCTC CAGTAAGGTG 
AAAGCCTCCC GCCGGCTGAC GGAGCTGGAG AACGAGCTGA ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839743.1
CDS	22..5472
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984289.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984289.2

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGACGGCT CCGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTCCAGTT AAGCGTCACC GAGGTCGGGA CGGAGAAGTT CGATGACAGC 
TCGATCCAGT TGTTTGGTTC CGGAATCCAG CCCCACCACT GCGACCTCAC CAACATGGAC 
GGGGTGGTCA CTGTCACGCC GAGAAGCGTG GATGCTGAGA CCTACGTGGA AGGACAGCGC 
ATCTCGGAGA CGACCATGCT GCAGAGCGGG GTGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCGCTCGCCA AGAGGCCGGG GGACGGAGGC 
CTGGCCGTCA AGGGTCCCAG ACACAAACCG GGAATCGTTC AGGAGACAGC TTTCGATTTA 
GGAGGAGACG TTCACGGGGG GACGGCCGTA GCGAGCAGTA AGGGCTCCGC CCGACCGGAC 
GGCGACAGGG CCCCGTCTGC CTCCAGCAGC GCCGAGCGCG GGATGGTGAA GCCGATGGTG 
CGAATGGAGC CGCCGCAGGA GCCCCGCAGG CAGGACACCA GACCGCAGGA CGCCGCTGGG 
CCTGAGCTGA CGCTGCCCGC GAGCATCGAA TTCAGGGAAA GTGCTGAAGA CTCGTTCTTA 
TCCGCCATCA TCAATTACAC GAATAGCTCG ACGGTGCATT TTAAGTTGTC GCCTACGTAC 
GTGCTGTACA TGGCGTGTCG GCACGTGCTG TCCAGCCAGT ACCGCCCGGA CGTCAGCCCC 
GCCGAGCGCA CGCACAAGGT CATCGCCATC GTCAACAAGA TGGTGAGCAT GATGGACGGC 
GTCATCCAGG AAGTAGACCA GGTGGCTGAG AAACAGAAGA ACATCGCGGG GGCGCTTGCG 
TTCTGGATGG CCAACGCGTC GGAACTCCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGAATCACAC TGGACGCCCA GGATGTTTTG GCTCACCTGG TTCAGATGGC CTTTAAATAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAC AACTACATGC CGGCCTTTCT GGACGACCCC 
GAGGAGAACA GTCTGCAAAG ACCAAAAATA GAGGACGTCC TCCACACGCT CACGGGCGCC 
ATGTCGCTGC TGCGGCGCTG CCGGGTCAAC GCCGCCCTCA CCATCCAGCT CTTCTCCCAG 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACAGGCTGC TGACCGACCC CGAGTCGGGC 
CTGTGCTCCC ACTACTGGGG CGCCATCGTC CGCCAGCAGC TGGGCCACGT GGAGGCCTGG 
GCGGAGAAGC AGGGCCTGGA GCTGGCTGCC GACTGCCACC TCAGCAGGGT CGTGCAGGCG 
ACCACCCTGC TCACGATGGA CAAGTACGCC CCGGAGGACG CGGCCGGCAT CAACAGCACC 
TGCTTCAAGC TGAACTCGCT GCAGCTGCAG GCCTTGCTGC ACAACTACCA CTGTGCGCCC 
GACGAGCCCC TGGTCCCGCC GGATCTGATA GAAAGCGTGG TGGCCGTGGC TGAAAACACG 
GCGGACGAGC TGGCGCGCAG CGACGGCAGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCCT TCCTGCTGCC GGAGGACGGC TACTCGTGTG ACGTCGTCAG AAACATCCCC 
AACGGCTTGC AGGAGTTTTT GGATCCTTTG TGCCAGAGAG GGTTTTGCAG GCTGACGCCC 
CAGCCGCGCT CTCCGGGTTC CTGGACGATA CATTTTGAAG GTGCGGAGTA CGAGAGCCGC 
CTCGCACGGG AGAGCACGGA GCTGGCTCAG CCTCTGAGGA AAGAACCCGA AGTCATCACT 
GTGACCCTGA AGAAGCAGAA CGGGATGGGC CTCAGCATCG TCGCAGCGAA GGGCGCAGGT 
CAAGACAAAC TCGGGATCTA CGTCAAGTCG GTGGTGAGAG GAGGAGCTGC AGACGTGGAC 
GGGCGGCTGG CGGCGGGGGA CCAGCTCCTC AGTGTGGACG GACGAAGCCT GGTGGGCCTC 
TCTCAGGAAA GGGCAGCAGA GCTGATGACA AGGACAAGTT CCGTGGTGAC CCTGGAGGTA 
GCGAAGCAGG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC CTCGCCCATG 
ATGCAGAGAA TTTCAGACCG GCGTGGATCA GGTAAACCCC GACCAAAGAG CGAAGGTTTT 
GAGCTCTATA ATAACTCAGC TCAGAACGGG TCCCCCGAGA GCCCCCCGCT GCCCTGGGCC 
GAGTACAGCG AGCCAAAGAA GCTGCCTGGC GACGAGAGGC TGACGAAGAA CCGCGCCGAC 
CACCGCTCCA GCCCCAACGT TGCAAATCAG CCTCCTAGTC CTGGTGGGAA AGGTGCACAT 
GCCGCTGGAA CAGCAGCCAA GATCACATCC GTCTCCACTG GCAACCTCTG CACCGAGGAG 
CAGACGCCCC CGCCGGGGAC GGAGGCCTAC CCCATCCCCA CGCAGGCCTA CCCCCGAGAG 
TACTTCACCT TCCCGGCCTC CAGGTCCCAG GACCGCGTGG TCCCCCCCCA GAGCCAGTGG 
CCAAGCTACG AGGAGAAGCC GCACGCGGAC AGCAGCCACT CCAGCCTCGC GGTTCAGCGG 
GTGACCCGCT CCCAGGAGGA GCTGCGGGAG GACGCGGGGG CCTACCCACT GCACAGGATG 
GAGGCGGTGA TGGACCGCAA GTCTGACAGC GACATGTGGG TCGCCCAGAG CTCCTCGATG 
GGCTCCAGCG CGTCCAGCCA GGAGCACCTG GACCCCTCTT CCAAGGCGGG CACCCCCGCG 
TCCACCCTCA CCAAGAGCGG CCCGGGGCGC TGGAAGACCC CTGCAGTTCT GCAGCCCGCG 
CCCGGCCCCC AGCCGCTCCG GACCGACCTG CCCACGCACT ACGCCGGCGA CGTGGACGGC 
GTCCCCGTGG ACCTGCCCCT CCCGCCGCCT CCGGCTGCCG CGGGCCAGGG ACTCGCGGGG 
GGCCAAGGGG CCGCCGAGCG GAAGAAGCGG GAGGAGCAGC AGCGCTGGTA CGAGAAGGAG 
AAGGCGCGCC TGGAGGAGGA GCGTGAGCGC AAGCGGCGCG AGCAGGAGCG CAAGCTGGGC 
CAGATGCGCT CGCAGGCGCT GCTCCCCGCC CCGCTCTCCC CCGGCGCCAC CAAGCCCGCC 
GCCCCGGGGC CCCAGGACAC CGTCATCCGG GAGCTGCAGC CTCAGCAGCA GCCGCGCACC 
ATCGAGCGCA GGGACCTGCA GTACATCACC GTCAGCCGGG AGGAGCTCTC GGCCGGCGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGCGAC GCGCGGGAGA AGCTGGAGAA GCAGCAGCAG 
ATGCACGTGG TGGACCTGCT GAGCCGGGAG ATTCACGAGC TGCAGAGCAA GGCCGAGCGC 
TCGGCGGAGG AGAGCGGCCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGCGG 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGACGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTGGAGGCC GAGCGCAGAG CGCGGCAGAC TGCTGTGCCA 
GCAATCTCTG TTCTAGATTT GTTGCAGGAC GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG 
GAGATGCGGC AGCGGGAGGC GGAGGAGCGT GCCCGCCACG AGGAGGAGCG GCGGCGCCAG 
GAGGAGGAGC GGCTGAAGCG CGAGGCCGAG GAAAAGAGGC GCCAGGAGGA GGGGTACTAC 
AGCCGCCTGG AGGCCGAGCG GCGCAGACAG CACGAGGAGG CCGAGCGCCG GCTGCTGGAG 
CCCGAGGAGC CGGGCCTGGG CCGCCCCCCG CTCCCGCGGG GCTACGAGCC CCCCGGCCCC 
GCGCCGCCGC CCCCGCCCCA GCGCGCCGCC TCCTACCTGC AGGCCCAGGT CCTGTCCCCC 
GACTCCCTGT ACACCGCCAA GTTCGTGGCT TACGACGACG ATGACGAGGA GGAGGACGGC 
CCTGCAGGTC AGGATAAGTA CGCCCGCGCC AGGGGGGCCC GCGGGGGCCC CCGCCCGGCC 
TCCGATGGCA TCTTTCTCGG CAGCTCCGCC CAGGCGCCCT GGGCCAGGGC CAACAGTCCT 
GCGCACAGGG CAGGCGCGCC CCCGGCCCCG CCGCCCAAGC CCGGCCCCGC CCGAGGCCGA 
GGGCCCGCCT CGTACATGGG ATCTACGGGG ACGCTGATGG GCGCCCACGA TGGCTCTCGG 
GACCCAAGAG AGACGCTGTC CAAAAGCCAG GACGCGGACG TGCCGGGCGG GCCTGAGAGC 
CTGACCTTCC GGGAGCGCCA GCGCCTCTTC TCCCAGGGCC ACGAGGTCTC CAGTAAGGTG 
AAAGCCTCCC GCCGGCTGAC GGAGCTGGAG AACGAGCTGA ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_021085815.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020941474.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

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CloneID	OSe229413
Clone ID Related Accession (Same CDS sequence)	XM_021085815.1
Accession Version	XM_021085815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA CGAGAAGCCC CTAGTTGTGC AGCTGAATTG GAATAAAGAT 
GACCGCGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGACG CCGCTCCTCC CAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGACGGCT CCGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTCCAGTT AAGCGTCACC GAGGTCGGGA CGGAGAAGTT CGATGACAGC 
TCGATCCAGT TGTTTGGTTC CGGAATCCAG CCCCACCACT GCGACCTCAC CAACATGGAC 
GGGGTGGTCA CTGTCACGCC GAGAAGCGTG GATGCTGAGA CCTACGTGGA AGGACAGCGC 
ATCTCGGAGA CGACCATGCT GCAGAGCGGG GTGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCGCTCGCCA AGAGGCCGGG GGACGGAGGC 
CTGGCCGTCA AGGGTCCCAG ACACAAACCG GGAATCGTTC AGGAGACAGC TTTCGATTTA 
GGAGGAGACG TTCACGGGGG GACGGCCGTA GCGAGCAGTA AGGGCTCCGC CCGACCGGAC 
GGCGACAGGG CCCCGTCTGC CTCCAGCAGC GCCGAGCGCG GGATGGTGAA GCCGATGGTG 
CGAATGGAGC CGCCGCAGGA GCCCCGCAGG CAGGACACCA GACCGCAGGA CGCCGCTGGG 
CCTGAGCTGA CGCTGCCCGC GAGCATCGAA TTCAGGGAAA GTGCTGAAGA CTCGTTCTTA 
TCCGCCATCA TCAATTACAC GAATAGCTCG ACGGTGCATT TTAAGTTGTC GCCTACGTAC 
GTGCTGTACA TGGCGTGTCG GCACGTGCTG TCCAGCCAGT ACCGCCCGGA CGTCAGCCCC 
GCCGAGCGCA CGCACAAGGT CATCGCCATC GTCAACAAGA TGGTGAGCAT GATGGACGGC 
GTCATCCAGA AACAGAAGAA CATCGCGGGG GCGCTTGCGT TCTGGATGGC CAACGCGTCG 
GAACTCCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GAATCACACT GGACGCCCAG 
GATGTTTTGG CTCACCTGGT TCAGATGGCC TTTAAATACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAACA ACTACATGCC GGCCTTTCTG GACGACCCCG AGGAGAACAG TCTGCAAAGA 
CCAAAAATAG AGGACGTCCT CCACACGCTC ACGGGCGCCA TGTCGCTGCT GCGGCGCTGC 
CGGGTCAACG CCGCCCTCAC CATCCAGCTC TTCTCCCAGC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACAGGCTGCT GACCGACCCC GAGTCGGGCC TGTGCTCCCA CTACTGGGGC 
GCCATCGTCC GCCAGCAGCT GGGCCACGTG GAGGCCTGGG CGGAGAAGCA GGGCCTGGAG 
CTGGCTGCCG ACTGCCACCT CAGCAGGGTC GTGCAGGCGA CCACCCTGCT CACGATGGAC 
AAGTACGCCC CGGAGGACGC GGCCGGCATC AACAGCACCT GCTTCAAGCT GAACTCGCTG 
CAGCTGCAGG CCTTGCTGCA CAACTACCAC TGTGCGCCCG ACGAGCCCCT GGTCCCGCCG 
GATCTGATAG AAAGCGTGGT GGCCGTGGCT GAAAACACGG CGGACGAGCT GGCGCGCAGC 
GACGGCAGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCCTT CCTGCTGCCG 
GAGGACGGCT ACTCGTGTGA CGTCGTCAGA AACATCCCCA ACGGCTTGCA GGAGTTTTTG 
GATCCTTTGT GCCAGAGAGG GTTTTGCAGG CTGACGCCCC AGCCGCGCTC TCCGGGTTCC 
TGGACGATAC ATTTTGAAGG TGCGGAGTAC GAGAGCCGCC TCGCACGGGA GAGCACGGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCCGAA GTCATCACTG TGACCCTGAA GAAGCAGAAC 
GGGATGGGCC TCAGCATCGT CGCAGCGAAG GGCGCAGGTC AAGACAAACT CGGGATCTAC 
GTCAAGTCGG TGGTGAGAGG AGGAGCTGCA GACGTGGACG GGCGGCTGGC GGCGGGGGAC 
CAGCTCCTCA GTGTGGACGG ACGAAGCCTG GTGGGCCTCT CTCAGGAAAG GGCAGCAGAG 
CTGATGACAA GGACAAGTTC CGTGGTGACC CTGGAGGTAG CGAAGCAGGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCC TCGCCCATGA TGCAGAGAAT TTCAGACCGG 
CGTGGATCAG GTAAACCCCG ACCAAAGAGC GAAGGTTTTG AGCTCTATAA TAACTCAGCT 
CAGAACGGGT CCCCCGAGAG CCCCCCGCTG CCCTGGGCCG AGTACAGCGA GCCAAAGAAG 
CTGCCTGGCG ACGAGAGGCT GACGAAGAAC CGCGCCGACC ACCGCTCCAG CCCCAACGTT 
GCAAATCAGC CTCCTAGTCC TGGTGGGAAA GGTGCACATG CCGCTGGAAC AGCAGCCAAG 
ATCACATCCG TCTCCACTGG CAACCTCTGC ACCGAGGAGC AGACGCCCCC GCCGGGGACG 
GAGGCCTACC CCATCCCCAC GCAGGCCTAC CCCCGAGAGT ACTTCACCTT CCCGGCCTCC 
AGGTCCCAGG ACCGCGTGGT CCCCCCCCAG AGCCAGTGGC CAAGCTACGA GGAGAAGCCG 
CACGCGGACA GCAGCCACTC CAGCCTCGCG GTTCAGCGGG TGACCCGCTC CCAGGAGGAG 
CTGCGGGAGG ACGCGGGGGC CTACCCACTG CACAGGATGG AGGCGGTGAT GGACCGCAAG 
TCTGACAGCG ACATGTGGGT CGCCCAGAGC TCCTCGATGG GCTCCAGCGC GTCCAGCCAG 
GAGCACCTGG ACCCCTCTTC CAAGGCGGGC ACCCCCGCGT CCACCCTCAC CAAGAGCGGC 
CCGGGGCGCT GGAAGACCCC TGCAGTTCTG CAGCCCGCGC CCGGCCCCCA GCCGCTCCGG 
ACCGACCTGC CCACGCACTA CGCCGGCGAC GTGGACGGCG TCCCCGTGGA CCTGCCCCTC 
CCGCCGCCTC CGGCTGCCGC GGGCCAGGGA CTCGCGGGGG GCCAAGGGGC CGCCGAGCGG 
AAGAAGCGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCGCGCCT GGAGGAGGAG 
CGTGAGCGCA AGCGGCGCGA GCAGGAGCGC AAGCTGGGCC AGATGCGCTC GCAGGCGCTG 
CTCCCCGCCC CGCTCTCCCC CGGCGCCACC AAGCCCGCCG CCCCGGGGCC CCAGGACACC 
GTCATCCGGG AGCTGCAGCC TCAGCAGCAG CCGCGCACCA TCGAGCGCAG GGACCTGCAG 
TACATCACCG TCAGCCGGGA GGAGCTCTCG GCCGGCGACA GCCTGTCCCC CGACCCCTGG 
AAGCGCGACG CGCGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACGTGGT GGACCTGCTG 
AGCCGGGAGA TTCACGAGCT GCAGAGCAAG GCCGAGCGCT CGGCGGAGGA GAGCGGCCGC 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGCGGC TGCAGGAGTC CAAGCAGAAG 
GACGAGGACG ACGACGAGGA GGAGGACGAC GACGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCCG AGCGCAGAGC GCGGCAGACT GCTGTGCCAG CAATCTCTGT TCTAGATTTG 
TTGCAGGACG AGGAGCGGCG GCGGCAGCAG CAGCTGGAGG AGATGCGGCA GCGGGAGGCG 
GAGGAGCGTG CCCGCCACGA GGAGGAGCGG CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC 
GAGGCCGAGG AAAAGAGGCG CCAGGAGGAG GGGTACTACA GCCGCCTGGA GGCCGAGCGG 
CGCAGACAGC ACGAGGAGGC CGAGCGCCGG CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC 
CGCCCCCCGC TCCCGCGGGG CTACGAGCCC CCCGGCCCCG CGCCGCCGCC CCCGCCCCAG 
CGCGCCGCCT CCTACCTGCA GGCCCAGGTC CTGTCCCCCG ACTCCCTGTA CACCGCCAAG 
TTCGTGGCTT ACGACGACGA TGACGAGGAG GAGGACGGCC CTGCAGGTCA GGATAAGTAC 
GCCCGCGCCA GGGGGGCCCG CGGGGGCCCC CGCCCGGCCT CCGATGGCAT CTTTCTCGGC 
AGCTCCGCCC AGGCGCCCTG GGCCAGGGCC AACAGTCCTG CGCACAGGGC AGGCGCGCCC 
CCGGCCCCGC CGCCCAAGCC CGGCCCCGCC CGAGGCCGAG GGCCCGCCTC GTACATGGGA 
TCTACGGGGA CGCTGATGGG CGCCCACGAT GGCTCTCGGG ACCCAAGAGA GACGCTGTCC 
AAAAGCCAGG ACGCGGACGT GCCGGGCGGG CCTGAGAGCC TGACCTTCCG GGAGCGCCAG 
CGCCTCTTCT CCCAGGGCCA CGAGGTCTCC AGTAAGGTGA AAGCCTCCCG CCGGCTGACG 
GAGCTGGAGA ACGAGCTGAA CACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020941474.1
CDS	28..5577
Translation	MSAGARDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_021085815.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021085815.1

ORF Insert Sequence:

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA CGAGAAGCCC CTAGTTGTGC AGCTGAATTG GAATAAAGAT 
GACCGCGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGACG CCGCTCCTCC CAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGACGGCT CCGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTCCAGTT AAGCGTCACC GAGGTCGGGA CGGAGAAGTT CGATGACAGC 
TCGATCCAGT TGTTTGGTTC CGGAATCCAG CCCCACCACT GCGACCTCAC CAACATGGAC 
GGGGTGGTCA CTGTCACGCC GAGAAGCGTG GATGCTGAGA CCTACGTGGA AGGACAGCGC 
ATCTCGGAGA CGACCATGCT GCAGAGCGGG GTGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCGCTCGCCA AGAGGCCGGG GGACGGAGGC 
CTGGCCGTCA AGGGTCCCAG ACACAAACCG GGAATCGTTC AGGAGACAGC TTTCGATTTA 
GGAGGAGACG TTCACGGGGG GACGGCCGTA GCGAGCAGTA AGGGCTCCGC CCGACCGGAC 
GGCGACAGGG CCCCGTCTGC CTCCAGCAGC GCCGAGCGCG GGATGGTGAA GCCGATGGTG 
CGAATGGAGC CGCCGCAGGA GCCCCGCAGG CAGGACACCA GACCGCAGGA CGCCGCTGGG 
CCTGAGCTGA CGCTGCCCGC GAGCATCGAA TTCAGGGAAA GTGCTGAAGA CTCGTTCTTA 
TCCGCCATCA TCAATTACAC GAATAGCTCG ACGGTGCATT TTAAGTTGTC GCCTACGTAC 
GTGCTGTACA TGGCGTGTCG GCACGTGCTG TCCAGCCAGT ACCGCCCGGA CGTCAGCCCC 
GCCGAGCGCA CGCACAAGGT CATCGCCATC GTCAACAAGA TGGTGAGCAT GATGGACGGC 
GTCATCCAGA AACAGAAGAA CATCGCGGGG GCGCTTGCGT TCTGGATGGC CAACGCGTCG 
GAACTCCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GAATCACACT GGACGCCCAG 
GATGTTTTGG CTCACCTGGT TCAGATGGCC TTTAAATACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAACA ACTACATGCC GGCCTTTCTG GACGACCCCG AGGAGAACAG TCTGCAAAGA 
CCAAAAATAG AGGACGTCCT CCACACGCTC ACGGGCGCCA TGTCGCTGCT GCGGCGCTGC 
CGGGTCAACG CCGCCCTCAC CATCCAGCTC TTCTCCCAGC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACAGGCTGCT GACCGACCCC GAGTCGGGCC TGTGCTCCCA CTACTGGGGC 
GCCATCGTCC GCCAGCAGCT GGGCCACGTG GAGGCCTGGG CGGAGAAGCA GGGCCTGGAG 
CTGGCTGCCG ACTGCCACCT CAGCAGGGTC GTGCAGGCGA CCACCCTGCT CACGATGGAC 
AAGTACGCCC CGGAGGACGC GGCCGGCATC AACAGCACCT GCTTCAAGCT GAACTCGCTG 
CAGCTGCAGG CCTTGCTGCA CAACTACCAC TGTGCGCCCG ACGAGCCCCT GGTCCCGCCG 
GATCTGATAG AAAGCGTGGT GGCCGTGGCT GAAAACACGG CGGACGAGCT GGCGCGCAGC 
GACGGCAGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCCTT CCTGCTGCCG 
GAGGACGGCT ACTCGTGTGA CGTCGTCAGA AACATCCCCA ACGGCTTGCA GGAGTTTTTG 
GATCCTTTGT GCCAGAGAGG GTTTTGCAGG CTGACGCCCC AGCCGCGCTC TCCGGGTTCC 
TGGACGATAC ATTTTGAAGG TGCGGAGTAC GAGAGCCGCC TCGCACGGGA GAGCACGGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCCGAA GTCATCACTG TGACCCTGAA GAAGCAGAAC 
GGGATGGGCC TCAGCATCGT CGCAGCGAAG GGCGCAGGTC AAGACAAACT CGGGATCTAC 
GTCAAGTCGG TGGTGAGAGG AGGAGCTGCA GACGTGGACG GGCGGCTGGC GGCGGGGGAC 
CAGCTCCTCA GTGTGGACGG ACGAAGCCTG GTGGGCCTCT CTCAGGAAAG GGCAGCAGAG 
CTGATGACAA GGACAAGTTC CGTGGTGACC CTGGAGGTAG CGAAGCAGGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCC TCGCCCATGA TGCAGAGAAT TTCAGACCGG 
CGTGGATCAG GTAAACCCCG ACCAAAGAGC GAAGGTTTTG AGCTCTATAA TAACTCAGCT 
CAGAACGGGT CCCCCGAGAG CCCCCCGCTG CCCTGGGCCG AGTACAGCGA GCCAAAGAAG 
CTGCCTGGCG ACGAGAGGCT GACGAAGAAC CGCGCCGACC ACCGCTCCAG CCCCAACGTT 
GCAAATCAGC CTCCTAGTCC TGGTGGGAAA GGTGCACATG CCGCTGGAAC AGCAGCCAAG 
ATCACATCCG TCTCCACTGG CAACCTCTGC ACCGAGGAGC AGACGCCCCC GCCGGGGACG 
GAGGCCTACC CCATCCCCAC GCAGGCCTAC CCCCGAGAGT ACTTCACCTT CCCGGCCTCC 
AGGTCCCAGG ACCGCGTGGT CCCCCCCCAG AGCCAGTGGC CAAGCTACGA GGAGAAGCCG 
CACGCGGACA GCAGCCACTC CAGCCTCGCG GTTCAGCGGG TGACCCGCTC CCAGGAGGAG 
CTGCGGGAGG ACGCGGGGGC CTACCCACTG CACAGGATGG AGGCGGTGAT GGACCGCAAG 
TCTGACAGCG ACATGTGGGT CGCCCAGAGC TCCTCGATGG GCTCCAGCGC GTCCAGCCAG 
GAGCACCTGG ACCCCTCTTC CAAGGCGGGC ACCCCCGCGT CCACCCTCAC CAAGAGCGGC 
CCGGGGCGCT GGAAGACCCC TGCAGTTCTG CAGCCCGCGC CCGGCCCCCA GCCGCTCCGG 
ACCGACCTGC CCACGCACTA CGCCGGCGAC GTGGACGGCG TCCCCGTGGA CCTGCCCCTC 
CCGCCGCCTC CGGCTGCCGC GGGCCAGGGA CTCGCGGGGG GCCAAGGGGC CGCCGAGCGG 
AAGAAGCGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCGCGCCT GGAGGAGGAG 
CGTGAGCGCA AGCGGCGCGA GCAGGAGCGC AAGCTGGGCC AGATGCGCTC GCAGGCGCTG 
CTCCCCGCCC CGCTCTCCCC CGGCGCCACC AAGCCCGCCG CCCCGGGGCC CCAGGACACC 
GTCATCCGGG AGCTGCAGCC TCAGCAGCAG CCGCGCACCA TCGAGCGCAG GGACCTGCAG 
TACATCACCG TCAGCCGGGA GGAGCTCTCG GCCGGCGACA GCCTGTCCCC CGACCCCTGG 
AAGCGCGACG CGCGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACGTGGT GGACCTGCTG 
AGCCGGGAGA TTCACGAGCT GCAGAGCAAG GCCGAGCGCT CGGCGGAGGA GAGCGGCCGC 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGCGGC TGCAGGAGTC CAAGCAGAAG 
GACGAGGACG ACGACGAGGA GGAGGACGAC GACGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCCG AGCGCAGAGC GCGGCAGACT GCTGTGCCAG CAATCTCTGT TCTAGATTTG 
TTGCAGGACG AGGAGCGGCG GCGGCAGCAG CAGCTGGAGG AGATGCGGCA GCGGGAGGCG 
GAGGAGCGTG CCCGCCACGA GGAGGAGCGG CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC 
GAGGCCGAGG AAAAGAGGCG CCAGGAGGAG GGGTACTACA GCCGCCTGGA GGCCGAGCGG 
CGCAGACAGC ACGAGGAGGC CGAGCGCCGG CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC 
CGCCCCCCGC TCCCGCGGGG CTACGAGCCC CCCGGCCCCG CGCCGCCGCC CCCGCCCCAG 
CGCGCCGCCT CCTACCTGCA GGCCCAGGTC CTGTCCCCCG ACTCCCTGTA CACCGCCAAG 
TTCGTGGCTT ACGACGACGA TGACGAGGAG GAGGACGGCC CTGCAGGTCA GGATAAGTAC 
GCCCGCGCCA GGGGGGCCCG CGGGGGCCCC CGCCCGGCCT CCGATGGCAT CTTTCTCGGC 
AGCTCCGCCC AGGCGCCCTG GGCCAGGGCC AACAGTCCTG CGCACAGGGC AGGCGCGCCC 
CCGGCCCCGC CGCCCAAGCC CGGCCCCGCC CGAGGCCGAG GGCCCGCCTC GTACATGGGA 
TCTACGGGGA CGCTGATGGG CGCCCACGAT GGCTCTCGGG ACCCAAGAGA GACGCTGTCC 
AAAAGCCAGG ACGCGGACGT GCCGGGCGGG CCTGAGAGCC TGACCTTCCG GGAGCGCCAG 
CGCCTCTTCT CCCAGGGCCA CGAGGTCTCC AGTAAGGTGA AAGCCTCCCG CCGGCTGACG 
GAGCTGGAGA ACGAGCTGAA CACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984296.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839750.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

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CloneID	OSe229414
Clone ID Related Accession (Same CDS sequence)	XM_013984296.2
Accession Version	XM_013984296.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013984296.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGACGCGGCC GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG CAGACTGCTG TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGCAGCGG 
GAGGCGGAGG AGCGTGCCCG CCACGAGGAG GAGCGGCGGC GCCAGGAGGA GGAGCGGCTG 
AAGCGCGAGG CCGAGGAAAA GAGGCGCCAG GAGGAGGGGT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCA GACAGCACGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CCCCGCTCCC GCGGGGCTAC GAGCCCCCCG GCCCCGCGCC GCCGCCCCCG 
CCCCAGCGCG CCGCCTCCTA CCTGCAGGCC CAGGTCCTGT CCCCCGACTC CCTGTACACC 
GCCAAGTTCG TGGCTTACGA CGACGATGAC GAGGAGGAGG ACGGCCCTGC AGGGCCCGCC 
TCGTACATGG GATCTACGGG GACGCTGATG GGCGCCCACG ATGGCTCTCG GGACCCAAGA 
GAGACGCTGT CCAAAAGCCA GGACGCGGAC GTGCCGGGCG GGCCTGAGAG CCTGACCTTC 
CGGGAGCGCC AGCGCCTCTT CTCCCAGGGC CACGAGGTCT CCAGTAAGGT GAAAGCCTCC 
CGCCGGCTGA CGGAGCTGGA GAACGAGCTG AACACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839750.1
CDS	22..5343
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984296.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984296.2

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGACGCGGCC GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG CAGACTGCTG TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGCAGCGG 
GAGGCGGAGG AGCGTGCCCG CCACGAGGAG GAGCGGCGGC GCCAGGAGGA GGAGCGGCTG 
AAGCGCGAGG CCGAGGAAAA GAGGCGCCAG GAGGAGGGGT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCA GACAGCACGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CCCCGCTCCC GCGGGGCTAC GAGCCCCCCG GCCCCGCGCC GCCGCCCCCG 
CCCCAGCGCG CCGCCTCCTA CCTGCAGGCC CAGGTCCTGT CCCCCGACTC CCTGTACACC 
GCCAAGTTCG TGGCTTACGA CGACGATGAC GAGGAGGAGG ACGGCCCTGC AGGGCCCGCC 
TCGTACATGG GATCTACGGG GACGCTGATG GGCGCCCACG ATGGCTCTCG GGACCCAAGA 
GAGACGCTGT CCAAAAGCCA GGACGCGGAC GTGCCGGGCG GGCCTGAGAG CCTGACCTTC 
CGGGAGCGCC AGCGCCTCTT CTCCCAGGGC CACGAGGTCT CCAGTAAGGT GAAAGCCTCC 
CGCCGGCTGA CGGAGCTGGA GAACGAGCTG AACACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_021085824.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020941483.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

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CloneID	OSe229415
Clone ID Related Accession (Same CDS sequence)	XM_021085824.1
Accession Version	XM_021085824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGACGCGGCC GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG TTGCAGGACG AGGAGCGGCG GCGGCAGCAG 
CAGCTGGAGG AGATGCGGCA GCGGGAGGCG GAGGAGCGTG CCCGCCACGA GGAGGAGCGG 
CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC GAGGCCGAGG AAAAGAGGCG CCAGGAGGAG 
GGGTACTACA GCCGCCTGGA GGCCGAGCGG CGCAGACAGC ACGAGGAGGC CGAGCGCCGG 
CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC CGCCCCCCGC TCCCGCGGGG CTACGAGCCC 
CCCGGCCCCG CGCCGCCGCC CCCGCCCCAG CGCGCCGCCT CCTACCTGCA GGCCCAGGTC 
CTGTCCCCCG ACTCCCTGTA CACCGCCAAG TTCGTGGCTT ACGACGACGA TGACGAGGAG 
GAGGACGGCC CTGCAGGGCC CGCCTCGTAC ATGGGATCTA CGGGGACGCT GATGGGCGCC 
CACGATGGCT CTCGGGACCC AAGAGAGACG CTGTCCAAAA GCCAGGACGC GGACGTGCCG 
GGCGGGCCTG AGAGCCTGAC CTTCCGGGAG CGCCAGCGCC TCTTCTCCCA GGGCCACGAG 
GTCTCCAGTA AGGTGAAAGC CTCCCGCCGG CTGACGGAGC TGGAGAACGA GCTGAACACC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020941483.1
CDS	22..5307
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_021085824.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021085824.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGACGCGGCC GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG TTGCAGGACG AGGAGCGGCG GCGGCAGCAG 
CAGCTGGAGG AGATGCGGCA GCGGGAGGCG GAGGAGCGTG CCCGCCACGA GGAGGAGCGG 
CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC GAGGCCGAGG AAAAGAGGCG CCAGGAGGAG 
GGGTACTACA GCCGCCTGGA GGCCGAGCGG CGCAGACAGC ACGAGGAGGC CGAGCGCCGG 
CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC CGCCCCCCGC TCCCGCGGGG CTACGAGCCC 
CCCGGCCCCG CGCCGCCGCC CCCGCCCCAG CGCGCCGCCT CCTACCTGCA GGCCCAGGTC 
CTGTCCCCCG ACTCCCTGTA CACCGCCAAG TTCGTGGCTT ACGACGACGA TGACGAGGAG 
GAGGACGGCC CTGCAGGGCC CGCCTCGTAC ATGGGATCTA CGGGGACGCT GATGGGCGCC 
CACGATGGCT CTCGGGACCC AAGAGAGACG CTGTCCAAAA GCCAGGACGC GGACGTGCCG 
GGCGGGCCTG AGAGCCTGAC CTTCCGGGAG CGCCAGCGCC TCTTCTCCCA GGGCCACGAG 
GTCTCCAGTA AGGTGAAAGC CTCCCGCCGG CTGACGGAGC TGGAGAACGA GCTGAACACC 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_021085831.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020941490.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

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CloneID	OSe229416
Clone ID Related Accession (Same CDS sequence)	XM_021085831.1
Accession Version	XM_021085831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA CGAGAAGCCC CTAGTTGTGC AGCTGAATTG GAATAAAGAT 
GACCGCGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGACG CCGCTCCTCC CAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAGA AAGAGGCGAT GAGACAAGCA 
TCTGATAAAG ATGATAGACC CTTCCACGGG GAGGACACTG AGAATTCTCG ACTGGCTGCC 
GAGGTTTACA AAGACATGCC CGAAACTAGC TTTACCCGAA CAATCTCCAA CCCCGAGGTG 
GTCATGAAGC GAAGGCGGCA GCAAAAACTG GAAAAGAGGA TGCAGGAGTT TCGGAGCTCA 
GACGGGCGCC CTGATTCCGG TGGAACGTTG AGAATTTATG CGGATAGTTT AAAGCCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGACCCCG CCGACTTTGC CGTGGCGGAG 
GCTTTGGAGA AGTACGGTCT GGAGAAGGAG AACCCGAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAGAAAGGTG CTAAAGAAAT TATCCTGGAC 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAATGGCCTG GTGACAAAGG GATGCTGGTC 
TTCCAGCTGA AGAGGAGGCC CCCAGAGCAC ATCCCCAAGA AGAGCAAGAA GCACGCGGAG 
GGGAAGCCGC CCAAGGGGAA GGAGCGGCCC GACGGCTCTG GCTATGGCTC TGCGCTCCCC 
CCGGAGAAGC TGCCCTACCT AGTGGAGCTG AGCCCAGGGA GAAGGGGTCA CTTTGCCTAC 
TACAGCTATC ACCCTCACGA AGACGGCTCC GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTCCAGTTAA GCGTCACCGA GGTCGGGACG GAGAAGTTCG ATGACAGCTC GATCCAGTTG 
TTTGGTTCCG GAATCCAGCC CCACCACTGC GACCTCACCA ACATGGACGG GGTGGTCACT 
GTCACGCCGA GAAGCGTGGA TGCTGAGACC TACGTGGAAG GACAGCGCAT CTCGGAGACG 
ACCATGCTGC AGAGCGGGGT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC GCTCGCCAAG AGGCCGGGGG ACGGAGGCCT GGCCGTCAAG 
GGTCCCAGAC ACAAACCGGG AATCGTTCAG GAGACAGCTT TCGATTTAGG AGGAGACGTT 
CACGGGGGGA CGGCCGTAGC GAGCAGTAAG GGCTCCGCCC GACCGGACGG CGACAGGGCC 
CCGTCTGCCT CCAGCAGCGC CGAGCGCGGG ATGGTGAAGC CGATGGTGCG AATGGAGCCG 
CCGCAGGAGC CCCGCAGGCA GGACACCAGA CCGCAGGACG CCGCTGGGCC TGAGCTGACG 
CTGCCCGCGA GCATCGAATT CAGGGAAAGT GCTGAAGACT CGTTCTTATC CGCCATCATC 
AATTACACGA ATAGCTCGAC GGTGCATTTT AAGTTGTCGC CTACGTACGT GCTGTACATG 
GCGTGTCGGC ACGTGCTGTC CAGCCAGTAC CGCCCGGACG TCAGCCCCGC CGAGCGCACG 
CACAAGGTCA TCGCCATCGT CAACAAGATG GTGAGCATGA TGGACGGCGT CATCCAGAAA 
CAGAAGAACA TCGCGGGGGC GCTTGCGTTC TGGATGGCCA ACGCGTCGGA ACTCCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGA ATCACACTGG ACGCCCAGGA TGTTTTGGCT 
CACCTGGTTC AGATGGCCTT TAAATACCTG GTTCACTGTC TTCAGTCAGA GCTGAACAAC 
TACATGCCGG CCTTTCTGGA CGACCCCGAG GAGAACAGTC TGCAAAGACC AAAAATAGAG 
GACGTCCTCC ACACGCTCAC GGGCGCCATG TCGCTGCTGC GGCGCTGCCG GGTCAACGCC 
GCCCTCACCA TCCAGCTCTT CTCCCAGCTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
AGGCTGCTGA CCGACCCCGA GTCGGGCCTG TGCTCCCACT ACTGGGGCGC CATCGTCCGC 
CAGCAGCTGG GCCACGTGGA GGCCTGGGCG GAGAAGCAGG GCCTGGAGCT GGCTGCCGAC 
TGCCACCTCA GCAGGGTCGT GCAGGCGACC ACCCTGCTCA CGATGGACAA GTACGCCCCG 
GAGGACGCGG CCGGCATCAA CAGCACCTGC TTCAAGCTGA ACTCGCTGCA GCTGCAGGCC 
TTGCTGCACA ACTACCACTG TGCGCCCGAC GAGCCCCTGG TCCCGCCGGA TCTGATAGAA 
AGCGTGGTGG CCGTGGCTGA AAACACGGCG GACGAGCTGG CGCGCAGCGA CGGCAGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TGCTGCCGGA GGACGGCTAC 
TCGTGTGACG TCGTCAGAAA CATCCCCAAC GGCTTGCAGG AGTTTTTGGA TCCTTTGTGC 
CAGAGAGGGT TTTGCAGGCT GACGCCCCAG CCGCGCTCTC CGGGTTCCTG GACGATACAT 
TTTGAAGGTG CGGAGTACGA GAGCCGCCTC GCACGGGAGA GCACGGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCCGAAGT CATCACTGTG ACCCTGAAGA AGCAGAACGG GATGGGCCTC 
AGCATCGTCG CAGCGAAGGG CGCAGGTCAA GACAAACTCG GGATCTACGT CAAGTCGGTG 
GTGAGAGGAG GAGCTGCAGA CGTGGACGGG CGGCTGGCGG CGGGGGACCA GCTCCTCAGT 
GTGGACGGAC GAAGCCTGGT GGGCCTCTCT CAGGAAAGGG CAGCAGAGCT GATGACAAGG 
ACAAGTTCCG TGGTGACCCT GGAGGTAGCG AAGCAGGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCCTC GCCCATGATG CAGAGAATTT CAGACCGGCG TGGATCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTTGAG CTCTATAATA ACTCAGCTCA GAACGGGTCC 
CCCGAGAGCC CCCCGCTGCC CTGGGCCGAG TACAGCGAGC CAAAGAAGCT GCCTGGCGAC 
GAGAGGCTGA CGAAGAACCG CGCCGACCAC CGCTCCAGCC CCAACGTTGC AAATCAGCCT 
CCTAGTCCTG GTGGGAAAGG TGCACATGCC GCTGGAACAG CAGCCAAGAT CACATCCGTC 
TCCACTGGCA ACCTCTGCAC CGAGGAGCAG ACGCCCCCGC CGGGGACGGA GGCCTACCCC 
ATCCCCACGC AGGCCTACCC CCGAGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGTCC CCCCCCAGAG CCAGTGGCCA AGCTACGAGG AGAAGCCGCA CGCGGACAGC 
AGCCACTCCA GCCTCGCGGT TCAGCGGGTG ACCCGCTCCC AGGAGGAGCT GCGGGAGGAC 
GCGGGGGCCT ACCCACTGCA CAGGATGGAG GCGGTGATGG ACCGCAAGTC TGACAGCGAC 
ATGTGGGTCG CCCAGAGCTC CTCGATGGGC TCCAGCGCGT CCAGCCAGGA GCACCTGGAC 
CCCTCTTCCA AGGCGGGCAC CCCCGCGTCC ACCCTCACCA AGAGCGGCCC GGGGCGCTGG 
AAGACCCCTG CAGTTCTGCA GCCCGCGCCC GGCCCCCAGC CGCTCCGGAC CGACCTGCCC 
ACGCACTACG CCGGCGACGT GGACGGCGTC CCCGTGGACC TGCCCCTCCC GCCGCCTCCG 
GCTGCCGCGG GCCAGGGACT CGCGGGGGGC CAAGGGGCCG CCGAGCGGAA GAAGCGGGAG 
GAGCAGCAGC GCTGGTACGA GAAGGAGAAG GCGCGCCTGG AGGAGGAGCG TGAGCGCAAG 
CGGCGCGAGC AGGAGCGCAA GCTGGGCCAG ATGCGCTCGC AGGCGCTGCT CCCCGCCCCG 
CTCTCCCCCG GCGCCACCAA GCCCGCCGCC CCGGGGCCCC AGGACACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC GCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACCGTC 
AGCCGGGAGG AGCTCTCGGC CGGCGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGACGCG 
CGGGAGAAGC TGGAGAAGCA GCAGCAGATG CACGTGGTGG ACCTGCTGAG CCGGGAGATT 
CACGAGCTGC AGAGCAAGGC CGAGCGCTCG GCGGAGGAGA GCGGCCGCCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GACGAGGAGG AGGACGACGA CGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGTTGCAGGA CGAGGAGCGG CGGCGGCAGC AGCAGCTGGA GGAGATGCGG 
CAGCGGGAGG CGGAGGAGCG TGCCCGCCAC GAGGAGGAGC GGCGGCGCCA GGAGGAGGAG 
CGGCTGAAGC GCGAGGCCGA GGAAAAGAGG CGCCAGGAGG AGGGGTACTA CAGCCGCCTG 
GAGGCCGAGC GGCGCAGACA GCACGAGGAG GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG 
CCGGGCCTGG GCCGCCCCCC GCTCCCGCGG GGCTACGAGC CCCCCGGCCC CGCGCCGCCG 
CCCCCGCCCC AGCGCGCCGC CTCCTACCTG CAGGCCCAGG TCCTGTCCCC CGACTCCCTG 
TACACCGCCA AGTTCGTGGC TTACGACGAC GATGACGAGG AGGAGGACGG CCCTGCAGGG 
CCCGCCTCGT ACATGGGATC TACGGGGACG CTGATGGGCG CCCACGATGG CTCTCGGGAC 
CCAAGAGAGA CGCTGTCCAA AAGCCAGGAC GCGGACGTGC CGGGCGGGCC TGAGAGCCTG 
ACCTTCCGGG AGCGCCAGCG CCTCTTCTCC CAGGGCCACG AGGTCTCCAG TAAGGTGAAA 
GCCTCCCGCC GGCTGACGGA GCTGGAGAAC GAGCTGAACA CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020941490.1
CDS	1..5388
Translation	MSAGARDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_021085831.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021085831.1

ORF Insert Sequence:

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA CGAGAAGCCC CTAGTTGTGC AGCTGAATTG GAATAAAGAT 
GACCGCGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGACG CCGCTCCTCC CAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAGA AAGAGGCGAT GAGACAAGCA 
TCTGATAAAG ATGATAGACC CTTCCACGGG GAGGACACTG AGAATTCTCG ACTGGCTGCC 
GAGGTTTACA AAGACATGCC CGAAACTAGC TTTACCCGAA CAATCTCCAA CCCCGAGGTG 
GTCATGAAGC GAAGGCGGCA GCAAAAACTG GAAAAGAGGA TGCAGGAGTT TCGGAGCTCA 
GACGGGCGCC CTGATTCCGG TGGAACGTTG AGAATTTATG CGGATAGTTT AAAGCCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGACCCCG CCGACTTTGC CGTGGCGGAG 
GCTTTGGAGA AGTACGGTCT GGAGAAGGAG AACCCGAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAGAAAGGTG CTAAAGAAAT TATCCTGGAC 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAATGGCCTG GTGACAAAGG GATGCTGGTC 
TTCCAGCTGA AGAGGAGGCC CCCAGAGCAC ATCCCCAAGA AGAGCAAGAA GCACGCGGAG 
GGGAAGCCGC CCAAGGGGAA GGAGCGGCCC GACGGCTCTG GCTATGGCTC TGCGCTCCCC 
CCGGAGAAGC TGCCCTACCT AGTGGAGCTG AGCCCAGGGA GAAGGGGTCA CTTTGCCTAC 
TACAGCTATC ACCCTCACGA AGACGGCTCC GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTCCAGTTAA GCGTCACCGA GGTCGGGACG GAGAAGTTCG ATGACAGCTC GATCCAGTTG 
TTTGGTTCCG GAATCCAGCC CCACCACTGC GACCTCACCA ACATGGACGG GGTGGTCACT 
GTCACGCCGA GAAGCGTGGA TGCTGAGACC TACGTGGAAG GACAGCGCAT CTCGGAGACG 
ACCATGCTGC AGAGCGGGGT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC GCTCGCCAAG AGGCCGGGGG ACGGAGGCCT GGCCGTCAAG 
GGTCCCAGAC ACAAACCGGG AATCGTTCAG GAGACAGCTT TCGATTTAGG AGGAGACGTT 
CACGGGGGGA CGGCCGTAGC GAGCAGTAAG GGCTCCGCCC GACCGGACGG CGACAGGGCC 
CCGTCTGCCT CCAGCAGCGC CGAGCGCGGG ATGGTGAAGC CGATGGTGCG AATGGAGCCG 
CCGCAGGAGC CCCGCAGGCA GGACACCAGA CCGCAGGACG CCGCTGGGCC TGAGCTGACG 
CTGCCCGCGA GCATCGAATT CAGGGAAAGT GCTGAAGACT CGTTCTTATC CGCCATCATC 
AATTACACGA ATAGCTCGAC GGTGCATTTT AAGTTGTCGC CTACGTACGT GCTGTACATG 
GCGTGTCGGC ACGTGCTGTC CAGCCAGTAC CGCCCGGACG TCAGCCCCGC CGAGCGCACG 
CACAAGGTCA TCGCCATCGT CAACAAGATG GTGAGCATGA TGGACGGCGT CATCCAGAAA 
CAGAAGAACA TCGCGGGGGC GCTTGCGTTC TGGATGGCCA ACGCGTCGGA ACTCCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGA ATCACACTGG ACGCCCAGGA TGTTTTGGCT 
CACCTGGTTC AGATGGCCTT TAAATACCTG GTTCACTGTC TTCAGTCAGA GCTGAACAAC 
TACATGCCGG CCTTTCTGGA CGACCCCGAG GAGAACAGTC TGCAAAGACC AAAAATAGAG 
GACGTCCTCC ACACGCTCAC GGGCGCCATG TCGCTGCTGC GGCGCTGCCG GGTCAACGCC 
GCCCTCACCA TCCAGCTCTT CTCCCAGCTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
AGGCTGCTGA CCGACCCCGA GTCGGGCCTG TGCTCCCACT ACTGGGGCGC CATCGTCCGC 
CAGCAGCTGG GCCACGTGGA GGCCTGGGCG GAGAAGCAGG GCCTGGAGCT GGCTGCCGAC 
TGCCACCTCA GCAGGGTCGT GCAGGCGACC ACCCTGCTCA CGATGGACAA GTACGCCCCG 
GAGGACGCGG CCGGCATCAA CAGCACCTGC TTCAAGCTGA ACTCGCTGCA GCTGCAGGCC 
TTGCTGCACA ACTACCACTG TGCGCCCGAC GAGCCCCTGG TCCCGCCGGA TCTGATAGAA 
AGCGTGGTGG CCGTGGCTGA AAACACGGCG GACGAGCTGG CGCGCAGCGA CGGCAGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TGCTGCCGGA GGACGGCTAC 
TCGTGTGACG TCGTCAGAAA CATCCCCAAC GGCTTGCAGG AGTTTTTGGA TCCTTTGTGC 
CAGAGAGGGT TTTGCAGGCT GACGCCCCAG CCGCGCTCTC CGGGTTCCTG GACGATACAT 
TTTGAAGGTG CGGAGTACGA GAGCCGCCTC GCACGGGAGA GCACGGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCCGAAGT CATCACTGTG ACCCTGAAGA AGCAGAACGG GATGGGCCTC 
AGCATCGTCG CAGCGAAGGG CGCAGGTCAA GACAAACTCG GGATCTACGT CAAGTCGGTG 
GTGAGAGGAG GAGCTGCAGA CGTGGACGGG CGGCTGGCGG CGGGGGACCA GCTCCTCAGT 
GTGGACGGAC GAAGCCTGGT GGGCCTCTCT CAGGAAAGGG CAGCAGAGCT GATGACAAGG 
ACAAGTTCCG TGGTGACCCT GGAGGTAGCG AAGCAGGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCCTC GCCCATGATG CAGAGAATTT CAGACCGGCG TGGATCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTTGAG CTCTATAATA ACTCAGCTCA GAACGGGTCC 
CCCGAGAGCC CCCCGCTGCC CTGGGCCGAG TACAGCGAGC CAAAGAAGCT GCCTGGCGAC 
GAGAGGCTGA CGAAGAACCG CGCCGACCAC CGCTCCAGCC CCAACGTTGC AAATCAGCCT 
CCTAGTCCTG GTGGGAAAGG TGCACATGCC GCTGGAACAG CAGCCAAGAT CACATCCGTC 
TCCACTGGCA ACCTCTGCAC CGAGGAGCAG ACGCCCCCGC CGGGGACGGA GGCCTACCCC 
ATCCCCACGC AGGCCTACCC CCGAGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGTCC CCCCCCAGAG CCAGTGGCCA AGCTACGAGG AGAAGCCGCA CGCGGACAGC 
AGCCACTCCA GCCTCGCGGT TCAGCGGGTG ACCCGCTCCC AGGAGGAGCT GCGGGAGGAC 
GCGGGGGCCT ACCCACTGCA CAGGATGGAG GCGGTGATGG ACCGCAAGTC TGACAGCGAC 
ATGTGGGTCG CCCAGAGCTC CTCGATGGGC TCCAGCGCGT CCAGCCAGGA GCACCTGGAC 
CCCTCTTCCA AGGCGGGCAC CCCCGCGTCC ACCCTCACCA AGAGCGGCCC GGGGCGCTGG 
AAGACCCCTG CAGTTCTGCA GCCCGCGCCC GGCCCCCAGC CGCTCCGGAC CGACCTGCCC 
ACGCACTACG CCGGCGACGT GGACGGCGTC CCCGTGGACC TGCCCCTCCC GCCGCCTCCG 
GCTGCCGCGG GCCAGGGACT CGCGGGGGGC CAAGGGGCCG CCGAGCGGAA GAAGCGGGAG 
GAGCAGCAGC GCTGGTACGA GAAGGAGAAG GCGCGCCTGG AGGAGGAGCG TGAGCGCAAG 
CGGCGCGAGC AGGAGCGCAA GCTGGGCCAG ATGCGCTCGC AGGCGCTGCT CCCCGCCCCG 
CTCTCCCCCG GCGCCACCAA GCCCGCCGCC CCGGGGCCCC AGGACACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC GCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACCGTC 
AGCCGGGAGG AGCTCTCGGC CGGCGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGACGCG 
CGGGAGAAGC TGGAGAAGCA GCAGCAGATG CACGTGGTGG ACCTGCTGAG CCGGGAGATT 
CACGAGCTGC AGAGCAAGGC CGAGCGCTCG GCGGAGGAGA GCGGCCGCCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GACGAGGAGG AGGACGACGA CGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGTTGCAGGA CGAGGAGCGG CGGCGGCAGC AGCAGCTGGA GGAGATGCGG 
CAGCGGGAGG CGGAGGAGCG TGCCCGCCAC GAGGAGGAGC GGCGGCGCCA GGAGGAGGAG 
CGGCTGAAGC GCGAGGCCGA GGAAAAGAGG CGCCAGGAGG AGGGGTACTA CAGCCGCCTG 
GAGGCCGAGC GGCGCAGACA GCACGAGGAG GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG 
CCGGGCCTGG GCCGCCCCCC GCTCCCGCGG GGCTACGAGC CCCCCGGCCC CGCGCCGCCG 
CCCCCGCCCC AGCGCGCCGC CTCCTACCTG CAGGCCCAGG TCCTGTCCCC CGACTCCCTG 
TACACCGCCA AGTTCGTGGC TTACGACGAC GATGACGAGG AGGAGGACGG CCCTGCAGGG 
CCCGCCTCGT ACATGGGATC TACGGGGACG CTGATGGGCG CCCACGATGG CTCTCGGGAC 
CCAAGAGAGA CGCTGTCCAA AAGCCAGGAC GCGGACGTGC CGGGCGGGCC TGAGAGCCTG 
ACCTTCCGGG AGCGCCAGCG CCTCTTCTCC CAGGGCCACG AGGTCTCCAG TAAGGTGAAA 
GCCTCCCGCC GGCTGACGGA GCTGGAGAAC GAGCTGAACA CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_021085833.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020941492.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

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CloneID	OSe229417
Clone ID Related Accession (Same CDS sequence)	XM_021085833.1
Accession Version	XM_021085833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAAA CTAGCTTTAC CCGAACAATC TCCAACCCCG AGGTGGTCAT GAAGCGAAGG 
CGGCAGCAAA AACTGGAAAA GAGGATGCAG GAGTTTCGGA GCTCAGACGG GCGCCCTGAT 
TCCGGTGGAA CGTTGAGAAT TTATGCGGAT AGTTTAAAGC CAAATATTCC CTACAAGACA 
ATCCTGCTGT CCACCACGGA CCCCGCCGAC TTTGCCGTGG CGGAGGCTTT GGAGAAGTAC 
GGTCTGGAGA AGGAGAACCC GAAGGATTAC TGCATCGCCC GGGTTATGCT TCCTCCTGGA 
GCCCAGCATT CGGATGAGAA AGGTGCTAAA GAAATTATCC TGGACGATGA CGAGTGTCCT 
TTACAGATCT TCAGGGAATG GCCTGGTGAC AAAGGGATGC TGGTCTTCCA GCTGAAGAGG 
AGGCCCCCAG AGCACATCCC CAAGAAGAGC AAGAAGCACG CGGAGGGGAA GCCGCCCAAG 
GGGAAGGAGC GGCCCGACGG CTCTGGCTAT GGCTCTGCGC TCCCCCCGGA GAAGCTGCCC 
TACCTAGTGG AGCTGAGCCC AGGGAGAAGG GGTCACTTTG CCTACTACAG CTATCACCCT 
CACGAAGACG GCTCCGACTC CAGAGATAAG CCAAAGCTTT ACCGCCTCCA GTTAAGCGTC 
ACCGAGGTCG GGACGGAGAA GTTCGATGAC AGCTCGATCC AGTTGTTTGG TTCCGGAATC 
CAGCCCCACC ACTGCGACCT CACCAACATG GACGGGGTGG TCACTGTCAC GCCGAGAAGC 
GTGGATGCTG AGACCTACGT GGAAGGACAG CGCATCTCGG AGACGACCAT GCTGCAGAGC 
GGGGTGAAGG TGCAGTTCGG GGCGTCCCAC GTGTTCAAGT TTGTGGACCC CAGCCAGGAC 
CACGCGCTCG CCAAGAGGCC GGGGGACGGA GGCCTGGCCG TCAAGGGTCC CAGACACAAA 
CCGGGAATCG TTCAGGAGAC AGCTTTCGAT TTAGGAGGAG ACGTTCACGG GGGGACGGCC 
GTAGCGAGCA GTAAGGGCTC CGCCCGACCG GACGGCGACA GGGCCCCGTC TGCCTCCAGC 
AGCGCCGAGC GCGGGATGGT GAAGCCGATG GTGCGAATGG AGCCGCCGCA GGAGCCCCGC 
AGGCAGGACA CCAGACCGCA GGACGCCGCT GGGCCTGAGC TGACGCTGCC CGCGAGCATC 
GAATTCAGGG AAAGTGCTGA AGACTCGTTC TTATCCGCCA TCATCAATTA CACGAATAGC 
TCGACGGTGC ATTTTAAGTT GTCGCCTACG TACGTGCTGT ACATGGCGTG TCGGCACGTG 
CTGTCCAGCC AGTACCGCCC GGACGTCAGC CCCGCCGAGC GCACGCACAA GGTCATCGCC 
ATCGTCAACA AGATGGTGAG CATGATGGAC GGCGTCATCC AGGAAGTAGA CCAGGTGGCT 
GAGAAACAGA AGAACATCGC GGGGGCGCTT GCGTTCTGGA TGGCCAACGC GTCGGAACTC 
CTCAACTTCA TCAAGCAGGA CCGAGACCTC AGTCGAATCA CACTGGACGC CCAGGATGTT 
TTGGCTCACC TGGTTCAGAT GGCCTTTAAA TACCTGGTTC ACTGTCTTCA GTCAGAGCTG 
AACAACTACA TGCCGGCCTT TCTGGACGAC CCCGAGGAGA ACAGTCTGCA AAGACCAAAA 
ATAGAGGACG TCCTCCACAC GCTCACGGGC GCCATGTCGC TGCTGCGGCG CTGCCGGGTC 
AACGCCGCCC TCACCATCCA GCTCTTCTCC CAGCTCTTCC ACTTCATCAA CATGTGGCTG 
TTCAACAGGC TGCTGACCGA CCCCGAGTCG GGCCTGTGCT CCCACTACTG GGGCGCCATC 
GTCCGCCAGC AGCTGGGCCA CGTGGAGGCC TGGGCGGAGA AGCAGGGCCT GGAGCTGGCT 
GCCGACTGCC ACCTCAGCAG GGTCGTGCAG GCGACCACCC TGCTCACGAT GGACAAGTAC 
GCCCCGGAGG ACGCGGCCGG CATCAACAGC ACCTGCTTCA AGCTGAACTC GCTGCAGCTG 
CAGGCCTTGC TGCACAACTA CCACTGTGCG CCCGACGAGC CCCTGGTCCC GCCGGATCTG 
ATAGAAAGCG TGGTGGCCGT GGCTGAAAAC ACGGCGGACG AGCTGGCGCG CAGCGACGGC 
AGGGAGGTGC GGCTGGAGGA GGACCCTGAC CTGCAGCTGC CCTTCCTGCT GCCGGAGGAC 
GGCTACTCGT GTGACGTCGT CAGAAACATC CCCAACGGCT TGCAGGAGTT TTTGGATCCT 
TTGTGCCAGA GAGGGTTTTG CAGGCTGACG CCCCAGCCGC GCTCTCCGGG TTCCTGGACG 
ATACATTTTG AAGGTGCGGA GTACGAGAGC CGCCTCGCAC GGGAGAGCAC GGAGCTGGCT 
CAGCCTCTGA GGAAAGAACC CGAAGTCATC ACTGTGACCC TGAAGAAGCA GAACGGGATG 
GGCCTCAGCA TCGTCGCAGC GAAGGGCGCA GGTCAAGACA AACTCGGGAT CTACGTCAAG 
TCGGTGGTGA GAGGAGGAGC TGCAGACGTG GACGGGCGGC TGGCGGCGGG GGACCAGCTC 
CTCAGTGTGG ACGGACGAAG CCTGGTGGGC CTCTCTCAGG AAAGGGCAGC AGAGCTGATG 
ACAAGGACAA GTTCCGTGGT GACCCTGGAG GTAGCGAAGC AGGGAGCCAT TTACCACGGC 
CTCGCCACCC TGCTCAACCA GCCCTCGCCC ATGATGCAGA GAATTTCAGA CCGGCGTGGA 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTTGAGCTCT ATAATAACTC AGCTCAGAAC 
GGGTCCCCCG AGAGCCCCCC GCTGCCCTGG GCCGAGTACA GCGAGCCAAA GAAGCTGCCT 
GGCGACGAGA GGCTGACGAA GAACCGCGCC GACCACCGCT CCAGCCCCAA CGTTGCAAAT 
CAGCCTCCTA GTCCTGGTGG GAAAGGTGCA CATGCCGCTG GAACAGCAGC CAAGATCACA 
TCCGTCTCCA CTGGCAACCT CTGCACCGAG GAGCAGACGC CCCCGCCGGG GACGGAGGCC 
TACCCCATCC CCACGCAGGC CTACCCCCGA GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGTCCCCCC CCAGAGCCAG TGGCCAAGCT ACGAGGAGAA GCCGCACGCG 
GACAGCAGCC ACTCCAGCCT CGCGGTTCAG CGGGTGACCC GCTCCCAGGA GGAGCTGCGG 
GAGGACGCGG GGGCCTACCC ACTGCACAGG ATGGAGGCGG TGATGGACCG CAAGTCTGAC 
AGCGACATGT GGGTCGCCCA GAGCTCCTCG ATGGGCTCCA GCGCGTCCAG CCAGGAGCAC 
CTGGACCCCT CTTCCAAGGC GGGCACCCCC GCGTCCACCC TCACCAAGAG CGGCCCGGGG 
CGCTGGAAGA CCCCTGCAGT TCTGCAGCCC GCGCCCGGCC CCCAGCCGCT CCGGACCGAC 
CTGCCCACGC ACTACGCCGG CGACGTGGAC GGCGTCCCCG TGGACCTGCC CCTCCCGCCG 
CCTCCGGCTG CCGCGGGCCA GGGACTCGCG GGGGGCCAAG GGGCCGCCGA GCGGAAGAAG 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCGC GCCTGGAGGA GGAGCGTGAG 
CGCAAGCGGC GCGAGCAGGA GCGCAAGCTG GGCCAGATGC GCTCGCAGGC GCTGCTCCCC 
GCCCCGCTCT CCCCCGGCGC CACCAAGCCC GCCGCCCCGG GGCCCCAGGA CACCGTCATC 
CGGGAGCTGC AGCCTCAGCA GCAGCCGCGC ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACCGTCAGCC GGGAGGAGCT CTCGGCCGGC GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GACGCGCGGG AGAAGCTGGA GAAGCAGCAG CAGATGCACG TGGTGGACCT GCTGAGCCGG 
GAGATTCACG AGCTGCAGAG CAAGGCCGAG CGCTCGGCGG AGGAGAGCGG CCGCCTGCGC 
AAGCTCATGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGACG AGGAGGAGGA CGACGACGTG GACACCATGC TGATCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTGTG CCAGCAATCT CTGTTCTAGA TTTGTTGCAG 
GACGAGGAGC GGCGGCGGCA GCAGCAGCTG GAGGAGATGC GGCAGCGGGA GGCGGAGGAG 
CGTGCCCGCC ACGAGGAGGA GCGGCGGCGC CAGGAGGAGG AGCGGCTGAA GCGCGAGGCC 
GAGGAAAAGA GGCGCCAGGA GGAGGGGTAC TACAGCCGCC TGGAGGCCGA GCGGCGCAGA 
CAGCACGAGG AGGCCGAGCG CCGGCTGCTG GAGCCCGAGG AGCCGGGCCT GGGCCGCCCC 
CCGCTCCCGC GGGGCTACGA GCCCCCCGGC CCCGCGCCGC CGCCCCCGCC CCAGCGCGCC 
GCCTCCTACC TGCAGGCCCA GGTCCTGTCC CCCGACTCCC TGTACACCGC CAAGTTCGTG 
GCTTACGACG ACGATGACGA GGAGGAGGAC GGCCCTGCAG GTCAGGATAA GTACGCCCGC 
GCCAGGGGGG CCCGCGGGGG CCCCCGCCCG GCCTCCGATG GCATCTTTCT CGGCAGCTCC 
GCCCAGGCGC CCTGGGCCAG GGCCAACAGT CCTGCGCACA GGGCAGGCGC GCCCCCGGCC 
CCGCCGCCCA AGCCCGGCCC CGCCCGAGGC CGAGGGCCCG CCTCGTACAT GGGATCTACG 
GGGACGCTGA TGGGCGCCCA CGATGGCTCT CGGGACCCAA GAGAGACGCT GTCCAAAAGC 
CAGGACGCGG ACGTGCCGGG CGGGCCTGAG AGCCTGACCT TCCGGGAGCG CCAGCGCCTC 
TTCTCCCAGG GCCACGAGGT CTCCAGTAAG GTGAAAGCCT CCCGCCGGCT GACGGAGCTG 
GAGAACGAGC TGAACACCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020941492.1
CDS	234..5237
Translation	MPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_021085833.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021085833.1

ORF Insert Sequence:

ATGCCCGAAA CTAGCTTTAC CCGAACAATC TCCAACCCCG AGGTGGTCAT GAAGCGAAGG 
CGGCAGCAAA AACTGGAAAA GAGGATGCAG GAGTTTCGGA GCTCAGACGG GCGCCCTGAT 
TCCGGTGGAA CGTTGAGAAT TTATGCGGAT AGTTTAAAGC CAAATATTCC CTACAAGACA 
ATCCTGCTGT CCACCACGGA CCCCGCCGAC TTTGCCGTGG CGGAGGCTTT GGAGAAGTAC 
GGTCTGGAGA AGGAGAACCC GAAGGATTAC TGCATCGCCC GGGTTATGCT TCCTCCTGGA 
GCCCAGCATT CGGATGAGAA AGGTGCTAAA GAAATTATCC TGGACGATGA CGAGTGTCCT 
TTACAGATCT TCAGGGAATG GCCTGGTGAC AAAGGGATGC TGGTCTTCCA GCTGAAGAGG 
AGGCCCCCAG AGCACATCCC CAAGAAGAGC AAGAAGCACG CGGAGGGGAA GCCGCCCAAG 
GGGAAGGAGC GGCCCGACGG CTCTGGCTAT GGCTCTGCGC TCCCCCCGGA GAAGCTGCCC 
TACCTAGTGG AGCTGAGCCC AGGGAGAAGG GGTCACTTTG CCTACTACAG CTATCACCCT 
CACGAAGACG GCTCCGACTC CAGAGATAAG CCAAAGCTTT ACCGCCTCCA GTTAAGCGTC 
ACCGAGGTCG GGACGGAGAA GTTCGATGAC AGCTCGATCC AGTTGTTTGG TTCCGGAATC 
CAGCCCCACC ACTGCGACCT CACCAACATG GACGGGGTGG TCACTGTCAC GCCGAGAAGC 
GTGGATGCTG AGACCTACGT GGAAGGACAG CGCATCTCGG AGACGACCAT GCTGCAGAGC 
GGGGTGAAGG TGCAGTTCGG GGCGTCCCAC GTGTTCAAGT TTGTGGACCC CAGCCAGGAC 
CACGCGCTCG CCAAGAGGCC GGGGGACGGA GGCCTGGCCG TCAAGGGTCC CAGACACAAA 
CCGGGAATCG TTCAGGAGAC AGCTTTCGAT TTAGGAGGAG ACGTTCACGG GGGGACGGCC 
GTAGCGAGCA GTAAGGGCTC CGCCCGACCG GACGGCGACA GGGCCCCGTC TGCCTCCAGC 
AGCGCCGAGC GCGGGATGGT GAAGCCGATG GTGCGAATGG AGCCGCCGCA GGAGCCCCGC 
AGGCAGGACA CCAGACCGCA GGACGCCGCT GGGCCTGAGC TGACGCTGCC CGCGAGCATC 
GAATTCAGGG AAAGTGCTGA AGACTCGTTC TTATCCGCCA TCATCAATTA CACGAATAGC 
TCGACGGTGC ATTTTAAGTT GTCGCCTACG TACGTGCTGT ACATGGCGTG TCGGCACGTG 
CTGTCCAGCC AGTACCGCCC GGACGTCAGC CCCGCCGAGC GCACGCACAA GGTCATCGCC 
ATCGTCAACA AGATGGTGAG CATGATGGAC GGCGTCATCC AGGAAGTAGA CCAGGTGGCT 
GAGAAACAGA AGAACATCGC GGGGGCGCTT GCGTTCTGGA TGGCCAACGC GTCGGAACTC 
CTCAACTTCA TCAAGCAGGA CCGAGACCTC AGTCGAATCA CACTGGACGC CCAGGATGTT 
TTGGCTCACC TGGTTCAGAT GGCCTTTAAA TACCTGGTTC ACTGTCTTCA GTCAGAGCTG 
AACAACTACA TGCCGGCCTT TCTGGACGAC CCCGAGGAGA ACAGTCTGCA AAGACCAAAA 
ATAGAGGACG TCCTCCACAC GCTCACGGGC GCCATGTCGC TGCTGCGGCG CTGCCGGGTC 
AACGCCGCCC TCACCATCCA GCTCTTCTCC CAGCTCTTCC ACTTCATCAA CATGTGGCTG 
TTCAACAGGC TGCTGACCGA CCCCGAGTCG GGCCTGTGCT CCCACTACTG GGGCGCCATC 
GTCCGCCAGC AGCTGGGCCA CGTGGAGGCC TGGGCGGAGA AGCAGGGCCT GGAGCTGGCT 
GCCGACTGCC ACCTCAGCAG GGTCGTGCAG GCGACCACCC TGCTCACGAT GGACAAGTAC 
GCCCCGGAGG ACGCGGCCGG CATCAACAGC ACCTGCTTCA AGCTGAACTC GCTGCAGCTG 
CAGGCCTTGC TGCACAACTA CCACTGTGCG CCCGACGAGC CCCTGGTCCC GCCGGATCTG 
ATAGAAAGCG TGGTGGCCGT GGCTGAAAAC ACGGCGGACG AGCTGGCGCG CAGCGACGGC 
AGGGAGGTGC GGCTGGAGGA GGACCCTGAC CTGCAGCTGC CCTTCCTGCT GCCGGAGGAC 
GGCTACTCGT GTGACGTCGT CAGAAACATC CCCAACGGCT TGCAGGAGTT TTTGGATCCT 
TTGTGCCAGA GAGGGTTTTG CAGGCTGACG CCCCAGCCGC GCTCTCCGGG TTCCTGGACG 
ATACATTTTG AAGGTGCGGA GTACGAGAGC CGCCTCGCAC GGGAGAGCAC GGAGCTGGCT 
CAGCCTCTGA GGAAAGAACC CGAAGTCATC ACTGTGACCC TGAAGAAGCA GAACGGGATG 
GGCCTCAGCA TCGTCGCAGC GAAGGGCGCA GGTCAAGACA AACTCGGGAT CTACGTCAAG 
TCGGTGGTGA GAGGAGGAGC TGCAGACGTG GACGGGCGGC TGGCGGCGGG GGACCAGCTC 
CTCAGTGTGG ACGGACGAAG CCTGGTGGGC CTCTCTCAGG AAAGGGCAGC AGAGCTGATG 
ACAAGGACAA GTTCCGTGGT GACCCTGGAG GTAGCGAAGC AGGGAGCCAT TTACCACGGC 
CTCGCCACCC TGCTCAACCA GCCCTCGCCC ATGATGCAGA GAATTTCAGA CCGGCGTGGA 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTTGAGCTCT ATAATAACTC AGCTCAGAAC 
GGGTCCCCCG AGAGCCCCCC GCTGCCCTGG GCCGAGTACA GCGAGCCAAA GAAGCTGCCT 
GGCGACGAGA GGCTGACGAA GAACCGCGCC GACCACCGCT CCAGCCCCAA CGTTGCAAAT 
CAGCCTCCTA GTCCTGGTGG GAAAGGTGCA CATGCCGCTG GAACAGCAGC CAAGATCACA 
TCCGTCTCCA CTGGCAACCT CTGCACCGAG GAGCAGACGC CCCCGCCGGG GACGGAGGCC 
TACCCCATCC CCACGCAGGC CTACCCCCGA GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGTCCCCCC CCAGAGCCAG TGGCCAAGCT ACGAGGAGAA GCCGCACGCG 
GACAGCAGCC ACTCCAGCCT CGCGGTTCAG CGGGTGACCC GCTCCCAGGA GGAGCTGCGG 
GAGGACGCGG GGGCCTACCC ACTGCACAGG ATGGAGGCGG TGATGGACCG CAAGTCTGAC 
AGCGACATGT GGGTCGCCCA GAGCTCCTCG ATGGGCTCCA GCGCGTCCAG CCAGGAGCAC 
CTGGACCCCT CTTCCAAGGC GGGCACCCCC GCGTCCACCC TCACCAAGAG CGGCCCGGGG 
CGCTGGAAGA CCCCTGCAGT TCTGCAGCCC GCGCCCGGCC CCCAGCCGCT CCGGACCGAC 
CTGCCCACGC ACTACGCCGG CGACGTGGAC GGCGTCCCCG TGGACCTGCC CCTCCCGCCG 
CCTCCGGCTG CCGCGGGCCA GGGACTCGCG GGGGGCCAAG GGGCCGCCGA GCGGAAGAAG 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCGC GCCTGGAGGA GGAGCGTGAG 
CGCAAGCGGC GCGAGCAGGA GCGCAAGCTG GGCCAGATGC GCTCGCAGGC GCTGCTCCCC 
GCCCCGCTCT CCCCCGGCGC CACCAAGCCC GCCGCCCCGG GGCCCCAGGA CACCGTCATC 
CGGGAGCTGC AGCCTCAGCA GCAGCCGCGC ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACCGTCAGCC GGGAGGAGCT CTCGGCCGGC GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GACGCGCGGG AGAAGCTGGA GAAGCAGCAG CAGATGCACG TGGTGGACCT GCTGAGCCGG 
GAGATTCACG AGCTGCAGAG CAAGGCCGAG CGCTCGGCGG AGGAGAGCGG CCGCCTGCGC 
AAGCTCATGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGACG AGGAGGAGGA CGACGACGTG GACACCATGC TGATCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTGTG CCAGCAATCT CTGTTCTAGA TTTGTTGCAG 
GACGAGGAGC GGCGGCGGCA GCAGCAGCTG GAGGAGATGC GGCAGCGGGA GGCGGAGGAG 
CGTGCCCGCC ACGAGGAGGA GCGGCGGCGC CAGGAGGAGG AGCGGCTGAA GCGCGAGGCC 
GAGGAAAAGA GGCGCCAGGA GGAGGGGTAC TACAGCCGCC TGGAGGCCGA GCGGCGCAGA 
CAGCACGAGG AGGCCGAGCG CCGGCTGCTG GAGCCCGAGG AGCCGGGCCT GGGCCGCCCC 
CCGCTCCCGC GGGGCTACGA GCCCCCCGGC CCCGCGCCGC CGCCCCCGCC CCAGCGCGCC 
GCCTCCTACC TGCAGGCCCA GGTCCTGTCC CCCGACTCCC TGTACACCGC CAAGTTCGTG 
GCTTACGACG ACGATGACGA GGAGGAGGAC GGCCCTGCAG GTCAGGATAA GTACGCCCGC 
GCCAGGGGGG CCCGCGGGGG CCCCCGCCCG GCCTCCGATG GCATCTTTCT CGGCAGCTCC 
GCCCAGGCGC CCTGGGCCAG GGCCAACAGT CCTGCGCACA GGGCAGGCGC GCCCCCGGCC 
CCGCCGCCCA AGCCCGGCCC CGCCCGAGGC CGAGGGCCCG CCTCGTACAT GGGATCTACG 
GGGACGCTGA TGGGCGCCCA CGATGGCTCT CGGGACCCAA GAGAGACGCT GTCCAAAAGC 
CAGGACGCGG ACGTGCCGGG CGGGCCTGAG AGCCTGACCT TCCGGGAGCG CCAGCGCCTC 
TTCTCCCAGG GCCACGAGGT CTCCAGTAAG GTGAAAGCCT CCCGCCGGCT GACGGAGCTG 
GAGAACGAGC TGAACACCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984302.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839756.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

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CloneID	OSe229418
Clone ID Related Accession (Same CDS sequence)	XM_013984302.2
Accession Version	XM_013984302.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013984302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGAAACAG 
AAGAACATCG CGGGGGCGCT TGCGTTCTGG ATGGCCAACG CGTCGGAACT CCTCAACTTC 
ATCAAGCAGG ACCGAGACCT CAGTCGAATC ACACTGGACG CCCAGGATGT TTTGGCTCAC 
CTGGTTCAGA TGGCCTTTAA ATACCTGGTT CACTGTCTTC AGTCAGAGCT GAACAACTAC 
ATGCCGGCCT TTCTGGACGA CCCCGAGGAG AACAGTCTGC AAAGACCAAA AATAGAGGAC 
GTCCTCCACA CGCTCACGGG CGCCATGTCG CTGCTGCGGC GCTGCCGGGT CAACGCCGCC 
CTCACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCATCA ACATGTGGCT GTTCAACAGG 
CTGCTGACCG ACCCCGAGTC GGGCCTGTGC TCCCACTACT GGGGCGCCAT CGTCCGCCAG 
CAGCTGGGCC ACGTGGAGGC CTGGGCGGAG AAGCAGGGCC TGGAGCTGGC TGCCGACTGC 
CACCTCAGCA GGGTCGTGCA GGCGACCACC CTGCTCACGA TGGACAAGTA CGCCCCGGAG 
GACGCGGCCG GCATCAACAG CACCTGCTTC AAGCTGAACT CGCTGCAGCT GCAGGCCTTG 
CTGCACAACT ACCACTGTGC GCCCGACGAG CCCCTGGTCC CGCCGGATCT GATAGAAAGC 
GTGGTGGCCG TGGCTGAAAA CACGGCGGAC GAGCTGGCGC GCAGCGACGG CAGGGAGGTG 
CGGCTGGAGG AGGACCCTGA CCTGCAGCTG CCCTTCCTGC TGCCGGAGGA CGGCTACTCG 
TGTGACGTCG TCAGAAACAT CCCCAACGGC TTGCAGGAGT TTTTGGATCC TTTGTGCCAG 
AGAGGGTTTT GCAGGCTGAC GCCCCAGCCG CGCTCTCCGG GTTCCTGGAC GATACATTTT 
GAAGGTGCGG AGTACGAGAG CCGCCTCGCA CGGGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CCGAAGTCAT CACTGTGACC CTGAAGAAGC AGAACGGGAT GGGCCTCAGC 
ATCGTCGCAG CGAAGGGCGC AGGTCAAGAC AAACTCGGGA TCTACGTCAA GTCGGTGGTG 
AGAGGAGGAG CTGCAGACGT GGACGGGCGG CTGGCGGCGG GGGACCAGCT CCTCAGTGTG 
GACGGACGAA GCCTGGTGGG CCTCTCTCAG GAAAGGGCAG CAGAGCTGAT GACAAGGACA 
AGTTCCGTGG TGACCCTGGA GGTAGCGAAG CAGGGAGCCA TTTACCACGG CCTCGCCACC 
CTGCTCAACC AGCCCTCGCC CATGATGCAG AGAATTTCAG ACCGGCGTGG ATCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTTGAGCTC TATAATAACT CAGCTCAGAA CGGGTCCCCC 
GAGAGCCCCC CGCTGCCCTG GGCCGAGTAC AGCGAGCCAA AGAAGCTGCC TGGCGACGAG 
AGGCTGACGA AGAACCGCGC CGACCACCGC TCCAGCCCCA ACGTTGCAAA TCAGCCTCCT 
AGTCCTGGTG GGAAAGGTGC ACATGCCGCT GGAACAGCAG CCAAGATCAC ATCCGTCTCC 
ACTGGCAACC TCTGCACCGA GGAGCAGACG CCCCCGCCGG GGACGGAGGC CTACCCCATC 
CCCACGCAGG CCTACCCCCG AGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGTCCCCC CCCAGAGCCA GTGGCCAAGC TACGAGGAGA AGCCGCACGC GGACAGCAGC 
CACTCCAGCC TCGCGGTTCA GCGGGTGACC CGCTCCCAGG AGGAGCTGCG GGAGGACGCG 
GGGGCCTACC CACTGCACAG GATGGAGGCG GTGATGGACC GCAAGTCTGA CAGCGACATG 
TGGGTCGCCC AGAGCTCCTC GATGGGCTCC AGCGCGTCCA GCCAGGAGCA CCTGGACCCC 
TCTTCCAAGG CGGGCACCCC CGCGTCCACC CTCACCAAGA GCGGCCCGGG GCGCTGGAAG 
ACCCCTGCAG TTCTGCAGCC CGCGCCCGGC CCCCAGCCGC TCCGGACCGA CCTGCCCACG 
CACTACGCCG GCGACGTGGA CGGCGTCCCC GTGGACCTGC CCCTCCCGCC GCCTCCGGCT 
GCCGCGGGCC AGGGACTCGC GGGGGGCCAA GGGGCCGCCG AGCGGAAGAA GCGGGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAGAAGGCG CGCCTGGAGG AGGAGCGTGA GCGCAAGCGG 
CGCGAGCAGG AGCGCAAGCT GGGCCAGATG CGCTCGCAGG CGCTGCTCCC CGCCCCGCTC 
TCCCCCGGCG CCACCAAGCC CGCCGCCCCG GGGCCCCAGG ACACCGTCAT CCGGGAGCTG 
CAGCCTCAGC AGCAGCCGCG CACCATCGAG CGCAGGGACC TGCAGTACAT CACCGTCAGC 
CGGGAGGAGC TCTCGGCCGG CGACAGCCTG TCCCCCGACC CCTGGAAGCG CGACGCGCGG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC GTGGTGGACC TGCTGAGCCG GGAGATTCAC 
GAGCTGCAGA GCAAGGCCGA GCGCTCGGCG GAGGAGAGCG GCCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAC 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG GCCCGCCTCG 
TACATGGGAT CTACGGGGAC GCTGATGGGC GCCCACGATG GCTCTCGGGA CCCAAGAGAG 
ACGCTGTCCA AAAGCCAGGA CGCGGACGTG CCGGGCGGGC CTGAGAGCCT GACCTTCCGG 
GAGCGCCAGC GCCTCTTCTC CCAGGGCCAC GAGGTCTCCA GTAAGGTGAA AGCCTCCCGC 
CGGCTGACGG AGCTGGAGAA CGAGCTGAAC ACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839756.1
CDS	22..5280
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984302.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984302.2

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGAAACAG 
AAGAACATCG CGGGGGCGCT TGCGTTCTGG ATGGCCAACG CGTCGGAACT CCTCAACTTC 
ATCAAGCAGG ACCGAGACCT CAGTCGAATC ACACTGGACG CCCAGGATGT TTTGGCTCAC 
CTGGTTCAGA TGGCCTTTAA ATACCTGGTT CACTGTCTTC AGTCAGAGCT GAACAACTAC 
ATGCCGGCCT TTCTGGACGA CCCCGAGGAG AACAGTCTGC AAAGACCAAA AATAGAGGAC 
GTCCTCCACA CGCTCACGGG CGCCATGTCG CTGCTGCGGC GCTGCCGGGT CAACGCCGCC 
CTCACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCATCA ACATGTGGCT GTTCAACAGG 
CTGCTGACCG ACCCCGAGTC GGGCCTGTGC TCCCACTACT GGGGCGCCAT CGTCCGCCAG 
CAGCTGGGCC ACGTGGAGGC CTGGGCGGAG AAGCAGGGCC TGGAGCTGGC TGCCGACTGC 
CACCTCAGCA GGGTCGTGCA GGCGACCACC CTGCTCACGA TGGACAAGTA CGCCCCGGAG 
GACGCGGCCG GCATCAACAG CACCTGCTTC AAGCTGAACT CGCTGCAGCT GCAGGCCTTG 
CTGCACAACT ACCACTGTGC GCCCGACGAG CCCCTGGTCC CGCCGGATCT GATAGAAAGC 
GTGGTGGCCG TGGCTGAAAA CACGGCGGAC GAGCTGGCGC GCAGCGACGG CAGGGAGGTG 
CGGCTGGAGG AGGACCCTGA CCTGCAGCTG CCCTTCCTGC TGCCGGAGGA CGGCTACTCG 
TGTGACGTCG TCAGAAACAT CCCCAACGGC TTGCAGGAGT TTTTGGATCC TTTGTGCCAG 
AGAGGGTTTT GCAGGCTGAC GCCCCAGCCG CGCTCTCCGG GTTCCTGGAC GATACATTTT 
GAAGGTGCGG AGTACGAGAG CCGCCTCGCA CGGGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CCGAAGTCAT CACTGTGACC CTGAAGAAGC AGAACGGGAT GGGCCTCAGC 
ATCGTCGCAG CGAAGGGCGC AGGTCAAGAC AAACTCGGGA TCTACGTCAA GTCGGTGGTG 
AGAGGAGGAG CTGCAGACGT GGACGGGCGG CTGGCGGCGG GGGACCAGCT CCTCAGTGTG 
GACGGACGAA GCCTGGTGGG CCTCTCTCAG GAAAGGGCAG CAGAGCTGAT GACAAGGACA 
AGTTCCGTGG TGACCCTGGA GGTAGCGAAG CAGGGAGCCA TTTACCACGG CCTCGCCACC 
CTGCTCAACC AGCCCTCGCC CATGATGCAG AGAATTTCAG ACCGGCGTGG ATCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTTGAGCTC TATAATAACT CAGCTCAGAA CGGGTCCCCC 
GAGAGCCCCC CGCTGCCCTG GGCCGAGTAC AGCGAGCCAA AGAAGCTGCC TGGCGACGAG 
AGGCTGACGA AGAACCGCGC CGACCACCGC TCCAGCCCCA ACGTTGCAAA TCAGCCTCCT 
AGTCCTGGTG GGAAAGGTGC ACATGCCGCT GGAACAGCAG CCAAGATCAC ATCCGTCTCC 
ACTGGCAACC TCTGCACCGA GGAGCAGACG CCCCCGCCGG GGACGGAGGC CTACCCCATC 
CCCACGCAGG CCTACCCCCG AGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGTCCCCC CCCAGAGCCA GTGGCCAAGC TACGAGGAGA AGCCGCACGC GGACAGCAGC 
CACTCCAGCC TCGCGGTTCA GCGGGTGACC CGCTCCCAGG AGGAGCTGCG GGAGGACGCG 
GGGGCCTACC CACTGCACAG GATGGAGGCG GTGATGGACC GCAAGTCTGA CAGCGACATG 
TGGGTCGCCC AGAGCTCCTC GATGGGCTCC AGCGCGTCCA GCCAGGAGCA CCTGGACCCC 
TCTTCCAAGG CGGGCACCCC CGCGTCCACC CTCACCAAGA GCGGCCCGGG GCGCTGGAAG 
ACCCCTGCAG TTCTGCAGCC CGCGCCCGGC CCCCAGCCGC TCCGGACCGA CCTGCCCACG 
CACTACGCCG GCGACGTGGA CGGCGTCCCC GTGGACCTGC CCCTCCCGCC GCCTCCGGCT 
GCCGCGGGCC AGGGACTCGC GGGGGGCCAA GGGGCCGCCG AGCGGAAGAA GCGGGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAGAAGGCG CGCCTGGAGG AGGAGCGTGA GCGCAAGCGG 
CGCGAGCAGG AGCGCAAGCT GGGCCAGATG CGCTCGCAGG CGCTGCTCCC CGCCCCGCTC 
TCCCCCGGCG CCACCAAGCC CGCCGCCCCG GGGCCCCAGG ACACCGTCAT CCGGGAGCTG 
CAGCCTCAGC AGCAGCCGCG CACCATCGAG CGCAGGGACC TGCAGTACAT CACCGTCAGC 
CGGGAGGAGC TCTCGGCCGG CGACAGCCTG TCCCCCGACC CCTGGAAGCG CGACGCGCGG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC GTGGTGGACC TGCTGAGCCG GGAGATTCAC 
GAGCTGCAGA GCAAGGCCGA GCGCTCGGCG GAGGAGAGCG GCCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAC 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG GCCCGCCTCG 
TACATGGGAT CTACGGGGAC GCTGATGGGC GCCCACGATG GCTCTCGGGA CCCAAGAGAG 
ACGCTGTCCA AAAGCCAGGA CGCGGACGTG CCGGGCGGGC CTGAGAGCCT GACCTTCCGG 
GAGCGCCAGC GCCTCTTCTC CCAGGGCCAC GAGGTCTCCA GTAAGGTGAA AGCCTCCCGC 
CGGCTGACGG AGCTGGAGAA CGAGCTGAAC ACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_021085839.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020941498.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

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CloneID	OSe229419
Clone ID Related Accession (Same CDS sequence)	XM_021085839.1
Accession Version	XM_021085839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA CGAGAAGCCC CTAGTTGTGC AGCTGAATTG GAATAAAGAT 
GACCGCGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGACG CCGCTCCTCC CAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGACGGCT CCGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTCCAGTT AAGCGTCACC GAGGTCGGGA CGGAGAAGTT CGATGACAGC 
TCGATCCAGT TGTTTGGTTC CGGAATCCAG CCCCACCACT GCGACCTCAC CAACATGGAC 
GGGGTGGTCA CTGTCACGCC GAGAAGCGTG GATGCTGAGA CCTACGTGGA AGGACAGCGC 
ATCTCGGAGA CGACCATGCT GCAGAGCGGG GTGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCGCTCGCCA AGAGGCCGGG GGACGGAGGC 
CTGGCCGTCA AGGGTCCCAG ACACAAACCG GGAATCGTTC AGGAGACAGC TTTCGATTTA 
GGAGGAGACG TTCACGGGGG GACGGCCGTA GCGAGCAGTA AGGGCTCCGC CCGACCGGAC 
GGCGACAGGG CCCCGTCTGC CTCCAGCAGC GCCGAGCGCG GGATGGTGAA GCCGATGGTG 
CGAATGGAGC CGCCGCAGGA GCCCCGCAGG CAGGACACCA GACCGCAGGA CGCCGCTGGG 
CCTGAGCTGA CGCTGCCCGC GAGCATCGAA TTCAGGGAAA GTGCTGAAGA CTCGTTCTTA 
TCCGCCATCA TCAATTACAC GAATAGCTCG ACGGTGCATT TTAAGTTGTC GCCTACGTAC 
GTGCTGTACA TGGCGTGTCG GCACGTGCTG TCCAGCCAGT ACCGCCCGGA CGTCAGCCCC 
GCCGAGCGCA CGCACAAGGT CATCGCCATC GTCAACAAGA TGGTGAGCAT GATGGACGGC 
GTCATCCAGA AACAGAAGAA CATCGCGGGG GCGCTTGCGT TCTGGATGGC CAACGCGTCG 
GAACTCCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GAATCACACT GGACGCCCAG 
GATGTTTTGG CTCACCTGGT TCAGATGGCC TTTAAATACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAACA ACTACATGCC GGCCTTTCTG GACGACCCCG AGGAGAACAG TCTGCAAAGA 
CCAAAAATAG AGGACGTCCT CCACACGCTC ACGGGCGCCA TGTCGCTGCT GCGGCGCTGC 
CGGGTCAACG CCGCCCTCAC CATCCAGCTC TTCTCCCAGC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACAGGCTGCT GACCGACCCC GAGTCGGGCC TGTGCTCCCA CTACTGGGGC 
GCCATCGTCC GCCAGCAGCT GGGCCACGTG GAGGCCTGGG CGGAGAAGCA GGGCCTGGAG 
CTGGCTGCCG ACTGCCACCT CAGCAGGGTC GTGCAGGCGA CCACCCTGCT CACGATGGAC 
AAGTACGCCC CGGAGGACGC GGCCGGCATC AACAGCACCT GCTTCAAGCT GAACTCGCTG 
CAGCTGCAGG CCTTGCTGCA CAACTACCAC TGTGCGCCCG ACGAGCCCCT GGTCCCGCCG 
GATCTGATAG AAAGCGTGGT GGCCGTGGCT GAAAACACGG CGGACGAGCT GGCGCGCAGC 
GACGGCAGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCCTT CCTGCTGCCG 
GAGGACGGCT ACTCGTGTGA CGTCGTCAGA AACATCCCCA ACGGCTTGCA GGAGTTTTTG 
GATCCTTTGT GCCAGAGAGG GTTTTGCAGG CTGACGCCCC AGCCGCGCTC TCCGGGTTCC 
TGGACGATAC ATTTTGAAGG TGCGGAGTAC GAGAGCCGCC TCGCACGGGA GAGCACGGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCCGAA GTCATCACTG TGACCCTGAA GAAGCAGAAC 
GGGATGGGCC TCAGCATCGT CGCAGCGAAG GGCGCAGGTC AAGACAAACT CGGGATCTAC 
GTCAAGTCGG TGGTGAGAGG AGGAGCTGCA GACGTGGACG GGCGGCTGGC GGCGGGGGAC 
CAGCTCCTCA GTGTGGACGG ACGAAGCCTG GTGGGCCTCT CTCAGGAAAG GGCAGCAGAG 
CTGATGACAA GGACAAGTTC CGTGGTGACC CTGGAGGTAG CGAAGCAGGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCC TCGCCCATGA TGCAGAGAAT TTCAGACCGG 
CGTGGATCAG GTAAACCCCG ACCAAAGAGC GAAGGTTTTG AGCTCTATAA TAACTCAGCT 
CAGAACGGGT CCCCCGAGAG CCCCCCGCTG CCCTGGGCCG AGTACAGCGA GCCAAAGAAG 
CTGCCTGGCG ACGAGAGGCT GACGAAGAAC CGCGCCGACC ACCGCTCCAG CCCCAACGTT 
GCAAATCAGC CTCCTAGTCC TGGTGGGAAA GGTGCACATG CCGCTGGAAC AGCAGCCAAG 
ATCACATCCG TCTCCACTGG CAACCTCTGC ACCGAGGAGC AGACGCCCCC GCCGGGGACG 
GAGGCCTACC CCATCCCCAC GCAGGCCTAC CCCCGAGAGT ACTTCACCTT CCCGGCCTCC 
AGGTCCCAGG ACCGCGTGGT CCCCCCCCAG AGCCAGTGGC CAAGCTACGA GGAGAAGCCG 
CACGCGGACA GCAGCCACTC CAGCCTCGCG GTTCAGCGGG TGACCCGCTC CCAGGAGGAG 
CTGCGGGAGG ACGCGGGGGC CTACCCACTG CACAGGATGG AGGCGGTGAT GGACCGCAAG 
TCTGACAGCG ACATGTGGGT CGCCCAGAGC TCCTCGATGG GCTCCAGCGC GTCCAGCCAG 
GAGCACCTGG ACCCCTCTTC CAAGGCGGGC ACCCCCGCGT CCACCCTCAC CAAGAGCGGC 
CCGGGGCGCT GGAAGACCCC TGCAGTTCTG CAGCCCGCGC CCGGCCCCCA GCCGCTCCGG 
ACCGACCTGC CCACGCACTA CGCCGGCGAC GTGGACGGCG TCCCCGTGGA CCTGCCCCTC 
CCGCCGCCTC CGGCTGCCGC GGGCCAGGGA CTCGCGGGGG GCCAAGGGGC CGCCGAGCGG 
AAGAAGCGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCGCGCCT GGAGGAGGAG 
CGTGAGCGCA AGCGGCGCGA GCAGGAGCGC AAGCTGGGCC AGATGCGCTC GCAGGCGCTG 
CTCCCCGCCC CGCTCTCCCC CGGCGCCACC AAGCCCGCCG CCCCGGGGCC CCAGGACACC 
GTCATCCGGG AGCTGCAGCC TCAGCAGCAG CCGCGCACCA TCGAGCGCAG GGACCTGCAG 
TACATCACCG TCAGCCGGGA GGAGCTCTCG GCCGGCGACA GCCTGTCCCC CGACCCCTGG 
AAGCGCGACG CGCGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACGTGGT GGACCTGCTG 
AGCCGGGAGA TTCACGAGCT GCAGAGCAAG GCCGAGCGCT CGGCGGAGGA GAGCGGCCGC 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGCGGC TGCAGGAGTC CAAGCAGAAG 
GACGAGGACG ACGACGAGGA GGAGGACGAC GACGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCCG AGCGCAGAGC GCGGTTGCAG GACGAGGAGC GGCGGCGGCA GCAGCAGCTG 
GAGGAGATGC GGCAGCGGGA GGCGGAGGAG CGTGCCCGCC ACGAGGAGGA GCGGCGGCGC 
CAGGAGGAGG AGCGGCTGAA GCGCGAGGCC GAGGAAAAGA GGCGCCAGGA GGAGGGGTAC 
TACAGCCGCC TGGAGGCCGA GCGGCGCAGA CAGCACGAGG AGGCCGAGCG CCGGCTGCTG 
GAGCCCGAGG AGCCGGGCCT GGGCCGCCCC CCGCTCCCGC GGGGCTACGA GCCCCCCGGC 
CCCGCGCCGC CGCCCCCGCC CCAGCGCGCC GCCTCCTACC TGCAGGCCCA GGTCCTGTCC 
CCCGACTCCC TGTACACCGC CAAGTTCGTG GCTTACGACG ACGATGACGA GGAGGAGGAC 
GGCCCTGCAG GTCAGGATAA GTACGCCCGC GCCAGGGGGG CCCGCGGGGG CCCCCGCCCG 
GCCTCCGATG GCATCTTTCT CGGCAGCTCC GCCCAGGCGC CCTGGGCCAG GGCCAACAGT 
CCTGCGCACA GGGCAGGCGC GCCCCCGGCC CCGCCGCCCA AGCCCGGCCC CGCCCGAGGC 
CGAGGGCCCG CCTCGTACAT GGGATCTACG GGGACGCTGA TGGGCGCCCA CGATGGCTCT 
CGGGACCCAA GAGAGACGCT GTCCAAAAGC CAGGACGCGG ACGTGCCGGG CGGGCCTGAG 
AGCCTGACCT TCCGGGAGCG CCAGCGCCTC TTCTCCCAGG GCCACGAGGT CTCCAGTAAG 
GTGAAAGCCT CCCGCCGGCT GACGGAGCTG GAGAACGAGC TGAACACCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020941498.1
CDS	27..5540
Translation	MSAGARDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_021085839.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021085839.1

ORF Insert Sequence:

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA CGAGAAGCCC CTAGTTGTGC AGCTGAATTG GAATAAAGAT 
GACCGCGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGACG CCGCTCCTCC CAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGACGGCT CCGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTCCAGTT AAGCGTCACC GAGGTCGGGA CGGAGAAGTT CGATGACAGC 
TCGATCCAGT TGTTTGGTTC CGGAATCCAG CCCCACCACT GCGACCTCAC CAACATGGAC 
GGGGTGGTCA CTGTCACGCC GAGAAGCGTG GATGCTGAGA CCTACGTGGA AGGACAGCGC 
ATCTCGGAGA CGACCATGCT GCAGAGCGGG GTGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCGCTCGCCA AGAGGCCGGG GGACGGAGGC 
CTGGCCGTCA AGGGTCCCAG ACACAAACCG GGAATCGTTC AGGAGACAGC TTTCGATTTA 
GGAGGAGACG TTCACGGGGG GACGGCCGTA GCGAGCAGTA AGGGCTCCGC CCGACCGGAC 
GGCGACAGGG CCCCGTCTGC CTCCAGCAGC GCCGAGCGCG GGATGGTGAA GCCGATGGTG 
CGAATGGAGC CGCCGCAGGA GCCCCGCAGG CAGGACACCA GACCGCAGGA CGCCGCTGGG 
CCTGAGCTGA CGCTGCCCGC GAGCATCGAA TTCAGGGAAA GTGCTGAAGA CTCGTTCTTA 
TCCGCCATCA TCAATTACAC GAATAGCTCG ACGGTGCATT TTAAGTTGTC GCCTACGTAC 
GTGCTGTACA TGGCGTGTCG GCACGTGCTG TCCAGCCAGT ACCGCCCGGA CGTCAGCCCC 
GCCGAGCGCA CGCACAAGGT CATCGCCATC GTCAACAAGA TGGTGAGCAT GATGGACGGC 
GTCATCCAGA AACAGAAGAA CATCGCGGGG GCGCTTGCGT TCTGGATGGC CAACGCGTCG 
GAACTCCTCA ACTTCATCAA GCAGGACCGA GACCTCAGTC GAATCACACT GGACGCCCAG 
GATGTTTTGG CTCACCTGGT TCAGATGGCC TTTAAATACC TGGTTCACTG TCTTCAGTCA 
GAGCTGAACA ACTACATGCC GGCCTTTCTG GACGACCCCG AGGAGAACAG TCTGCAAAGA 
CCAAAAATAG AGGACGTCCT CCACACGCTC ACGGGCGCCA TGTCGCTGCT GCGGCGCTGC 
CGGGTCAACG CCGCCCTCAC CATCCAGCTC TTCTCCCAGC TCTTCCACTT CATCAACATG 
TGGCTGTTCA ACAGGCTGCT GACCGACCCC GAGTCGGGCC TGTGCTCCCA CTACTGGGGC 
GCCATCGTCC GCCAGCAGCT GGGCCACGTG GAGGCCTGGG CGGAGAAGCA GGGCCTGGAG 
CTGGCTGCCG ACTGCCACCT CAGCAGGGTC GTGCAGGCGA CCACCCTGCT CACGATGGAC 
AAGTACGCCC CGGAGGACGC GGCCGGCATC AACAGCACCT GCTTCAAGCT GAACTCGCTG 
CAGCTGCAGG CCTTGCTGCA CAACTACCAC TGTGCGCCCG ACGAGCCCCT GGTCCCGCCG 
GATCTGATAG AAAGCGTGGT GGCCGTGGCT GAAAACACGG CGGACGAGCT GGCGCGCAGC 
GACGGCAGGG AGGTGCGGCT GGAGGAGGAC CCTGACCTGC AGCTGCCCTT CCTGCTGCCG 
GAGGACGGCT ACTCGTGTGA CGTCGTCAGA AACATCCCCA ACGGCTTGCA GGAGTTTTTG 
GATCCTTTGT GCCAGAGAGG GTTTTGCAGG CTGACGCCCC AGCCGCGCTC TCCGGGTTCC 
TGGACGATAC ATTTTGAAGG TGCGGAGTAC GAGAGCCGCC TCGCACGGGA GAGCACGGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCCGAA GTCATCACTG TGACCCTGAA GAAGCAGAAC 
GGGATGGGCC TCAGCATCGT CGCAGCGAAG GGCGCAGGTC AAGACAAACT CGGGATCTAC 
GTCAAGTCGG TGGTGAGAGG AGGAGCTGCA GACGTGGACG GGCGGCTGGC GGCGGGGGAC 
CAGCTCCTCA GTGTGGACGG ACGAAGCCTG GTGGGCCTCT CTCAGGAAAG GGCAGCAGAG 
CTGATGACAA GGACAAGTTC CGTGGTGACC CTGGAGGTAG CGAAGCAGGG AGCCATTTAC 
CACGGCCTCG CCACCCTGCT CAACCAGCCC TCGCCCATGA TGCAGAGAAT TTCAGACCGG 
CGTGGATCAG GTAAACCCCG ACCAAAGAGC GAAGGTTTTG AGCTCTATAA TAACTCAGCT 
CAGAACGGGT CCCCCGAGAG CCCCCCGCTG CCCTGGGCCG AGTACAGCGA GCCAAAGAAG 
CTGCCTGGCG ACGAGAGGCT GACGAAGAAC CGCGCCGACC ACCGCTCCAG CCCCAACGTT 
GCAAATCAGC CTCCTAGTCC TGGTGGGAAA GGTGCACATG CCGCTGGAAC AGCAGCCAAG 
ATCACATCCG TCTCCACTGG CAACCTCTGC ACCGAGGAGC AGACGCCCCC GCCGGGGACG 
GAGGCCTACC CCATCCCCAC GCAGGCCTAC CCCCGAGAGT ACTTCACCTT CCCGGCCTCC 
AGGTCCCAGG ACCGCGTGGT CCCCCCCCAG AGCCAGTGGC CAAGCTACGA GGAGAAGCCG 
CACGCGGACA GCAGCCACTC CAGCCTCGCG GTTCAGCGGG TGACCCGCTC CCAGGAGGAG 
CTGCGGGAGG ACGCGGGGGC CTACCCACTG CACAGGATGG AGGCGGTGAT GGACCGCAAG 
TCTGACAGCG ACATGTGGGT CGCCCAGAGC TCCTCGATGG GCTCCAGCGC GTCCAGCCAG 
GAGCACCTGG ACCCCTCTTC CAAGGCGGGC ACCCCCGCGT CCACCCTCAC CAAGAGCGGC 
CCGGGGCGCT GGAAGACCCC TGCAGTTCTG CAGCCCGCGC CCGGCCCCCA GCCGCTCCGG 
ACCGACCTGC CCACGCACTA CGCCGGCGAC GTGGACGGCG TCCCCGTGGA CCTGCCCCTC 
CCGCCGCCTC CGGCTGCCGC GGGCCAGGGA CTCGCGGGGG GCCAAGGGGC CGCCGAGCGG 
AAGAAGCGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCGCGCCT GGAGGAGGAG 
CGTGAGCGCA AGCGGCGCGA GCAGGAGCGC AAGCTGGGCC AGATGCGCTC GCAGGCGCTG 
CTCCCCGCCC CGCTCTCCCC CGGCGCCACC AAGCCCGCCG CCCCGGGGCC CCAGGACACC 
GTCATCCGGG AGCTGCAGCC TCAGCAGCAG CCGCGCACCA TCGAGCGCAG GGACCTGCAG 
TACATCACCG TCAGCCGGGA GGAGCTCTCG GCCGGCGACA GCCTGTCCCC CGACCCCTGG 
AAGCGCGACG CGCGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACGTGGT GGACCTGCTG 
AGCCGGGAGA TTCACGAGCT GCAGAGCAAG GCCGAGCGCT CGGCGGAGGA GAGCGGCCGC 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGCGGC TGCAGGAGTC CAAGCAGAAG 
GACGAGGACG ACGACGAGGA GGAGGACGAC GACGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCCG AGCGCAGAGC GCGGTTGCAG GACGAGGAGC GGCGGCGGCA GCAGCAGCTG 
GAGGAGATGC GGCAGCGGGA GGCGGAGGAG CGTGCCCGCC ACGAGGAGGA GCGGCGGCGC 
CAGGAGGAGG AGCGGCTGAA GCGCGAGGCC GAGGAAAAGA GGCGCCAGGA GGAGGGGTAC 
TACAGCCGCC TGGAGGCCGA GCGGCGCAGA CAGCACGAGG AGGCCGAGCG CCGGCTGCTG 
GAGCCCGAGG AGCCGGGCCT GGGCCGCCCC CCGCTCCCGC GGGGCTACGA GCCCCCCGGC 
CCCGCGCCGC CGCCCCCGCC CCAGCGCGCC GCCTCCTACC TGCAGGCCCA GGTCCTGTCC 
CCCGACTCCC TGTACACCGC CAAGTTCGTG GCTTACGACG ACGATGACGA GGAGGAGGAC 
GGCCCTGCAG GTCAGGATAA GTACGCCCGC GCCAGGGGGG CCCGCGGGGG CCCCCGCCCG 
GCCTCCGATG GCATCTTTCT CGGCAGCTCC GCCCAGGCGC CCTGGGCCAG GGCCAACAGT 
CCTGCGCACA GGGCAGGCGC GCCCCCGGCC CCGCCGCCCA AGCCCGGCCC CGCCCGAGGC 
CGAGGGCCCG CCTCGTACAT GGGATCTACG GGGACGCTGA TGGGCGCCCA CGATGGCTCT 
CGGGACCCAA GAGAGACGCT GTCCAAAAGC CAGGACGCGG ACGTGCCGGG CGGGCCTGAG 
AGCCTGACCT TCCGGGAGCG CCAGCGCCTC TTCTCCCAGG GCCACGAGGT CTCCAGTAAG 
GTGAAAGCCT CCCGCCGGCT GACGGAGCTG GAGAACGAGC TGAACACCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_021085843.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020941502.1 Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.

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CloneID	OSe229420
Clone ID Related Accession (Same CDS sequence)	XM_021085843.1
Accession Version	XM_021085843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	afadin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010443.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA CGAGAAGCCC CTAGTTGTGC AGCTGAATTG GAATAAAGAT 
GACCGCGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGACG CCGCTCCTCC CAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAGA AAGAGGCGAT GAGACAAGCA 
TCTGATAAAG ATGATAGACC CTTCCACGGG GAGGACACTG AGAATTCTCG ACTGGCTGCC 
GAGGTTTACA AAGACATGCC CGAAACTAGC TTTACCCGAA CAATCTCCAA CCCCGAGGTG 
GTCATGAAGC GAAGGCGGCA GCAAAAACTG GAAAAGAGGA TGCAGGAGTT TCGGAGCTCA 
GACGGGCGCC CTGATTCCGG TGGAACGTTG AGAATTTATG CGGATAGTTT AAAGCCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGACCCCG CCGACTTTGC CGTGGCGGAG 
GCTTTGGAGA AGTACGGTCT GGAGAAGGAG AACCCGAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAGAAAGGTG CTAAAGAAAT TATCCTGGAC 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAATGGCCTG GTGACAAAGG GATGCTGGTC 
TTCCAGCTGA AGAGGAGGCC CCCAGAGCAC ATCCCCAAGA AGAGCAAGAA GCACGCGGAG 
GGGAAGCCGC CCAAGGGGAA GGAGCGGCCC GACGGCTCTG GCTATGGCTC TGCGCTCCCC 
CCGGAGAAGC TGCCCTACCT AGTGGAGCTG AGCCCAGGGA GAAGGGGTCA CTTTGCCTAC 
TACAGCTATC ACCCTCACGA AGACGGCTCC GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTCCAGTTAA GCGTCACCGA GGTCGGGACG GAGAAGTTCG ATGACAGCTC GATCCAGTTG 
TTTGGTTCCG GAATCCAGCC CCACCACTGC GACCTCACCA ACATGGACGG GGTGGTCACT 
GTCACGCCGA GAAGCGTGGA TGCTGAGACC TACGTGGAAG GACAGCGCAT CTCGGAGACG 
ACCATGCTGC AGAGCGGGGT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC GCTCGCCAAG AGGCCGGGGG ACGGAGGCCT GGCCGTCAAG 
GGTCCCAGAC ACAAACCGGG AATCGTTCAG GAGACAGCTT TCGATTTAGG AGGAGACGTT 
CACGGGGGGA CGGCCGTAGC GAGCAGTAAG GGCTCCGCCC GACCGGACGG CGACAGGGCC 
CCGTCTGCCT CCAGCAGCGC CGAGCGCGGG ATGGTGAAGC CGATGGTGCG AATGGAGCCG 
CCGCAGGAGC CCCGCAGGCA GGACACCAGA CCGCAGGACG CCGCTGGGCC TGAGCTGACG 
CTGCCCGCGA GCATCGAATT CAGGGAAAGT GCTGAAGACT CGTTCTTATC CGCCATCATC 
AATTACACGA ATAGCTCGAC GGTGCATTTT AAGTTGTCGC CTACGTACGT GCTGTACATG 
GCGTGTCGGC ACGTGCTGTC CAGCCAGTAC CGCCCGGACG TCAGCCCCGC CGAGCGCACG 
CACAAGGTCA TCGCCATCGT CAACAAGATG GTGAGCATGA TGGACGGCGT CATCCAGAAA 
CAGAAGAACA TCGCGGGGGC GCTTGCGTTC TGGATGGCCA ACGCGTCGGA ACTCCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGA ATCACACTGG ACGCCCAGGA TGTTTTGGCT 
CACCTGGTTC AGATGGCCTT TAAATACCTG GTTCACTGTC TTCAGTCAGA GCTGAACAAC 
TACATGCCGG CCTTTCTGGA CGACCCCGAG GAGAACAGTC TGCAAAGACC AAAAATAGAG 
GACGTCCTCC ACACGCTCAC GGGCGCCATG TCGCTGCTGC GGCGCTGCCG GGTCAACGCC 
GCCCTCACCA TCCAGCTCTT CTCCCAGCTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
AGGCTGCTGA CCGACCCCGA GTCGGGCCTG TGCTCCCACT ACTGGGGCGC CATCGTCCGC 
CAGCAGCTGG GCCACGTGGA GGCCTGGGCG GAGAAGCAGG GCCTGGAGCT GGCTGCCGAC 
TGCCACCTCA GCAGGGTCGT GCAGGCGACC ACCCTGCTCA CGATGGACAA GTACGCCCCG 
GAGGACGCGG CCGGCATCAA CAGCACCTGC TTCAAGCTGA ACTCGCTGCA GCTGCAGGCC 
TTGCTGCACA ACTACCACTG TGCGCCCGAC GAGCCCCTGG TCCCGCCGGA TCTGATAGAA 
AGCGTGGTGG CCGTGGCTGA AAACACGGCG GACGAGCTGG CGCGCAGCGA CGGCAGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TGCTGCCGGA GGACGGCTAC 
TCGTGTGACG TCGTCAGAAA CATCCCCAAC GGCTTGCAGG AGTTTTTGGA TCCTTTGTGC 
CAGAGAGGGT TTTGCAGGCT GACGCCCCAG CCGCGCTCTC CGGGTTCCTG GACGATACAT 
TTTGAAGGTG CGGAGTACGA GAGCCGCCTC GCACGGGAGA GCACGGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCCGAAGT CATCACTGTG ACCCTGAAGA AGCAGAACGG GATGGGCCTC 
AGCATCGTCG CAGCGAAGGG CGCAGGTCAA GACAAACTCG GGATCTACGT CAAGTCGGTG 
GTGAGAGGAG GAGCTGCAGA CGTGGACGGG CGGCTGGCGG CGGGGGACCA GCTCCTCAGT 
GTGGACGGAC GAAGCCTGGT GGGCCTCTCT CAGGAAAGGG CAGCAGAGCT GATGACAAGG 
ACAAGTTCCG TGGTGACCCT GGAGGTAGCG AAGCAGGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCCTC GCCCATGATG CAGAGAATTT CAGACCGGCG TGGATCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTTGAG CTCTATAATA ACTCAGCTCA GAACGGGTCC 
CCCGAGAGCC CCCCGCTGCC CTGGGCCGAG TACAGCGAGC CAAAGAAGCT GCCTGGCGAC 
GAGAGGCTGA CGAAGAACCG CGCCGACCAC CGCTCCAGCC CCAACGTTGC AAATCAGCCT 
CCTAGTCCTG GTGGGAAAGG TGCACATGCC GCTGGAACAG CAGCCAAGAT CACATCCGTC 
TCCACTGGCA ACCTCTGCAC CGAGGAGCAG ACGCCCCCGC CGGGGACGGA GGCCTACCCC 
ATCCCCACGC AGGCCTACCC CCGAGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGTCC CCCCCCAGAG CCAGTGGCCA AGCTACGAGG AGAAGCCGCA CGCGGACAGC 
AGCCACTCCA GCCTCGCGGT TCAGCGGGTG ACCCGCTCCC AGGAGGAGCT GCGGGAGGAC 
GCGGGGGCCT ACCCACTGCA CAGGATGGAG GCGGTGATGG ACCGCAAGTC TGACAGCGAC 
ATGTGGGTCG CCCAGAGCTC CTCGATGGGC TCCAGCGCGT CCAGCCAGGA GCACCTGGAC 
CCCTCTTCCA AGGCGGGCAC CCCCGCGTCC ACCCTCACCA AGAGCGGCCC GGGGCGCTGG 
AAGACCCCTG CAGTTCTGCA GCCCGCGCCC GGCCCCCAGC CGCTCCGGAC CGACCTGCCC 
ACGCACTACG CCGGCGACGT GGACGGCGTC CCCGTGGACC TGCCCCTCCC GCCGCCTCCG 
GCTGCCGCGG GCCAGGGACT CGCGGGGGGC CAAGGGGCCG CCGAGCGGAA GAAGCGGGAG 
GAGCAGCAGC GCTGGTACGA GAAGGAGAAG GCGCGCCTGG AGGAGGAGCG TGAGCGCAAG 
CGGCGCGAGC AGGAGCGCAA GCTGGGCCAG ATGCGCTCGC AGGCGCTGCT CCCCGCCCCG 
CTCTCCCCCG GCGCCACCAA GCCCGCCGCC CCGGGGCCCC AGGACACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC GCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACCGTC 
AGCCGGGAGG AGCTCTCGGC CGGCGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGACGCG 
CGGGAGAAGC TGGAGAAGCA GCAGCAGATG CACGTGGTGG ACCTGCTGAG CCGGGAGATT 
CACGAGCTGC AGAGCAAGGC CGAGCGCTCG GCGGAGGAGA GCGGCCGCCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GACGAGGAGG AGGACGACGA CGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGGACGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGCAGCGG 
GAGGCGGAGG AGCGTGCCCG CCACGAGGAG GAGCGGCGGC GCCAGGAGGA GGAGCGGCTG 
AAGCGCGAGG CCGAGGAAAA GGTCATGGTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020941502.1
CDS	27..4922
Translation	MSAGARDEERRKLADIIQHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKVMVL

Target ORF information:

RefSeq Version	XM_021085843.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021085843.1

ORF Insert Sequence:

ATGTCGGCGG GCGCCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCAGCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAATTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACTGCCAC CACTCAGGAT GTGATCGAGA CGCTGGCCGA GAAGTTCCGG 
CCGGATATGC GGATGCTCTC GTCCCCCAAG TACTCGCTCT ACGAGGTGCA CGTCAGCGGA 
GAAAGAAGAT TGGATATAGA CGAGAAGCCC CTAGTTGTGC AGCTGAATTG GAATAAAGAT 
GACCGCGAGG GCAGGTTTGT CCTTAAGAAC GAGAACGACG CCGCTCCTCC CAAGAAGGCT 
CAAAGTAACG GACCTGAAAA GCAGGAAAAA GAAGGCGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAGA AAGAGGCGAT GAGACAAGCA 
TCTGATAAAG ATGATAGACC CTTCCACGGG GAGGACACTG AGAATTCTCG ACTGGCTGCC 
GAGGTTTACA AAGACATGCC CGAAACTAGC TTTACCCGAA CAATCTCCAA CCCCGAGGTG 
GTCATGAAGC GAAGGCGGCA GCAAAAACTG GAAAAGAGGA TGCAGGAGTT TCGGAGCTCA 
GACGGGCGCC CTGATTCCGG TGGAACGTTG AGAATTTATG CGGATAGTTT AAAGCCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGACCCCG CCGACTTTGC CGTGGCGGAG 
GCTTTGGAGA AGTACGGTCT GGAGAAGGAG AACCCGAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCGGAT GAGAAAGGTG CTAAAGAAAT TATCCTGGAC 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAATGGCCTG GTGACAAAGG GATGCTGGTC 
TTCCAGCTGA AGAGGAGGCC CCCAGAGCAC ATCCCCAAGA AGAGCAAGAA GCACGCGGAG 
GGGAAGCCGC CCAAGGGGAA GGAGCGGCCC GACGGCTCTG GCTATGGCTC TGCGCTCCCC 
CCGGAGAAGC TGCCCTACCT AGTGGAGCTG AGCCCAGGGA GAAGGGGTCA CTTTGCCTAC 
TACAGCTATC ACCCTCACGA AGACGGCTCC GACTCCAGAG ATAAGCCAAA GCTTTACCGC 
CTCCAGTTAA GCGTCACCGA GGTCGGGACG GAGAAGTTCG ATGACAGCTC GATCCAGTTG 
TTTGGTTCCG GAATCCAGCC CCACCACTGC GACCTCACCA ACATGGACGG GGTGGTCACT 
GTCACGCCGA GAAGCGTGGA TGCTGAGACC TACGTGGAAG GACAGCGCAT CTCGGAGACG 
ACCATGCTGC AGAGCGGGGT GAAGGTGCAG TTCGGGGCGT CCCACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC GCTCGCCAAG AGGCCGGGGG ACGGAGGCCT GGCCGTCAAG 
GGTCCCAGAC ACAAACCGGG AATCGTTCAG GAGACAGCTT TCGATTTAGG AGGAGACGTT 
CACGGGGGGA CGGCCGTAGC GAGCAGTAAG GGCTCCGCCC GACCGGACGG CGACAGGGCC 
CCGTCTGCCT CCAGCAGCGC CGAGCGCGGG ATGGTGAAGC CGATGGTGCG AATGGAGCCG 
CCGCAGGAGC CCCGCAGGCA GGACACCAGA CCGCAGGACG CCGCTGGGCC TGAGCTGACG 
CTGCCCGCGA GCATCGAATT CAGGGAAAGT GCTGAAGACT CGTTCTTATC CGCCATCATC 
AATTACACGA ATAGCTCGAC GGTGCATTTT AAGTTGTCGC CTACGTACGT GCTGTACATG 
GCGTGTCGGC ACGTGCTGTC CAGCCAGTAC CGCCCGGACG TCAGCCCCGC CGAGCGCACG 
CACAAGGTCA TCGCCATCGT CAACAAGATG GTGAGCATGA TGGACGGCGT CATCCAGAAA 
CAGAAGAACA TCGCGGGGGC GCTTGCGTTC TGGATGGCCA ACGCGTCGGA ACTCCTCAAC 
TTCATCAAGC AGGACCGAGA CCTCAGTCGA ATCACACTGG ACGCCCAGGA TGTTTTGGCT 
CACCTGGTTC AGATGGCCTT TAAATACCTG GTTCACTGTC TTCAGTCAGA GCTGAACAAC 
TACATGCCGG CCTTTCTGGA CGACCCCGAG GAGAACAGTC TGCAAAGACC AAAAATAGAG 
GACGTCCTCC ACACGCTCAC GGGCGCCATG TCGCTGCTGC GGCGCTGCCG GGTCAACGCC 
GCCCTCACCA TCCAGCTCTT CTCCCAGCTC TTCCACTTCA TCAACATGTG GCTGTTCAAC 
AGGCTGCTGA CCGACCCCGA GTCGGGCCTG TGCTCCCACT ACTGGGGCGC CATCGTCCGC 
CAGCAGCTGG GCCACGTGGA GGCCTGGGCG GAGAAGCAGG GCCTGGAGCT GGCTGCCGAC 
TGCCACCTCA GCAGGGTCGT GCAGGCGACC ACCCTGCTCA CGATGGACAA GTACGCCCCG 
GAGGACGCGG CCGGCATCAA CAGCACCTGC TTCAAGCTGA ACTCGCTGCA GCTGCAGGCC 
TTGCTGCACA ACTACCACTG TGCGCCCGAC GAGCCCCTGG TCCCGCCGGA TCTGATAGAA 
AGCGTGGTGG CCGTGGCTGA AAACACGGCG GACGAGCTGG CGCGCAGCGA CGGCAGGGAG 
GTGCGGCTGG AGGAGGACCC TGACCTGCAG CTGCCCTTCC TGCTGCCGGA GGACGGCTAC 
TCGTGTGACG TCGTCAGAAA CATCCCCAAC GGCTTGCAGG AGTTTTTGGA TCCTTTGTGC 
CAGAGAGGGT TTTGCAGGCT GACGCCCCAG CCGCGCTCTC CGGGTTCCTG GACGATACAT 
TTTGAAGGTG CGGAGTACGA GAGCCGCCTC GCACGGGAGA GCACGGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCCGAAGT CATCACTGTG ACCCTGAAGA AGCAGAACGG GATGGGCCTC 
AGCATCGTCG CAGCGAAGGG CGCAGGTCAA GACAAACTCG GGATCTACGT CAAGTCGGTG 
GTGAGAGGAG GAGCTGCAGA CGTGGACGGG CGGCTGGCGG CGGGGGACCA GCTCCTCAGT 
GTGGACGGAC GAAGCCTGGT GGGCCTCTCT CAGGAAAGGG CAGCAGAGCT GATGACAAGG 
ACAAGTTCCG TGGTGACCCT GGAGGTAGCG AAGCAGGGAG CCATTTACCA CGGCCTCGCC 
ACCCTGCTCA ACCAGCCCTC GCCCATGATG CAGAGAATTT CAGACCGGCG TGGATCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTTGAG CTCTATAATA ACTCAGCTCA GAACGGGTCC 
CCCGAGAGCC CCCCGCTGCC CTGGGCCGAG TACAGCGAGC CAAAGAAGCT GCCTGGCGAC 
GAGAGGCTGA CGAAGAACCG CGCCGACCAC CGCTCCAGCC CCAACGTTGC AAATCAGCCT 
CCTAGTCCTG GTGGGAAAGG TGCACATGCC GCTGGAACAG CAGCCAAGAT CACATCCGTC 
TCCACTGGCA ACCTCTGCAC CGAGGAGCAG ACGCCCCCGC CGGGGACGGA GGCCTACCCC 
ATCCCCACGC AGGCCTACCC CCGAGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGTCC CCCCCCAGAG CCAGTGGCCA AGCTACGAGG AGAAGCCGCA CGCGGACAGC 
AGCCACTCCA GCCTCGCGGT TCAGCGGGTG ACCCGCTCCC AGGAGGAGCT GCGGGAGGAC 
GCGGGGGCCT ACCCACTGCA CAGGATGGAG GCGGTGATGG ACCGCAAGTC TGACAGCGAC 
ATGTGGGTCG CCCAGAGCTC CTCGATGGGC TCCAGCGCGT CCAGCCAGGA GCACCTGGAC 
CCCTCTTCCA AGGCGGGCAC CCCCGCGTCC ACCCTCACCA AGAGCGGCCC GGGGCGCTGG 
AAGACCCCTG CAGTTCTGCA GCCCGCGCCC GGCCCCCAGC CGCTCCGGAC CGACCTGCCC 
ACGCACTACG CCGGCGACGT GGACGGCGTC CCCGTGGACC TGCCCCTCCC GCCGCCTCCG 
GCTGCCGCGG GCCAGGGACT CGCGGGGGGC CAAGGGGCCG CCGAGCGGAA GAAGCGGGAG 
GAGCAGCAGC GCTGGTACGA GAAGGAGAAG GCGCGCCTGG AGGAGGAGCG TGAGCGCAAG 
CGGCGCGAGC AGGAGCGCAA GCTGGGCCAG ATGCGCTCGC AGGCGCTGCT CCCCGCCCCG 
CTCTCCCCCG GCGCCACCAA GCCCGCCGCC CCGGGGCCCC AGGACACCGT CATCCGGGAG 
CTGCAGCCTC AGCAGCAGCC GCGCACCATC GAGCGCAGGG ACCTGCAGTA CATCACCGTC 
AGCCGGGAGG AGCTCTCGGC CGGCGACAGC CTGTCCCCCG ACCCCTGGAA GCGCGACGCG 
CGGGAGAAGC TGGAGAAGCA GCAGCAGATG CACGTGGTGG ACCTGCTGAG CCGGGAGATT 
CACGAGCTGC AGAGCAAGGC CGAGCGCTCG GCGGAGGAGA GCGGCCGCCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGCGGCTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GACGAGGAGG AGGACGACGA CGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCCGAG 
CGCAGAGCGC GGGACGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGCAGCGG 
GAGGCGGAGG AGCGTGCCCG CCACGAGGAG GAGCGGCGGC GCCAGGAGGA GGAGCGGCTG 
AAGCGCGAGG CCGAGGAAAA GGTCATGGTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984265.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839719.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe30605
Clone ID Related Accession (Same CDS sequence)	XM_013984265.1
Accession Version	XM_013984265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGATGCGGCT GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG CAGACTGCTG TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGCAGCGG 
GAGGCGGAGG AGCGTGCCCG CCACGAGGAG GAGCGGCGGC GCCAGGAGGA GGAGCGGCTG 
AAGCGCGAGG CCGAGGAAAA GAGGCGCCAG GAGGAGGGGT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCA GACAGCACGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CCCCGCTCCC GCGGGGCTAC GAGCCCCCCG GCCCCGCGCC GCCGCCCCCG 
CCCCAGCGCG CCGCCTCCTA CCTGCAGGCC CAGGTCCTGT CCCCCGACTC CCTGTACACC 
GCCAAGTTCG TGGCTTACGA CGACGATGAC GAGGAGGAGG ACGGCCCTGC AGGTCAGGAT 
AAGTACGCCC GCGCCAGGGG GGCCCGCGGG GGCCCGCGCC CGGCCTCCGA TGGCATCTTT 
CTCGGCAGCT CCGCCCAGGC GCCCTGGGCC AGGGCCAACA GTCCTGCGCA CAGGGCAGGC 
GCGCCCCCGG CCCCGCCGCC CAAGCCCGGC CCCGCCCGAG GCCGAGGGCC CGCCTCGTAC 
ATGGGATCTA CGGGGACGCT GATGGGCGCC CACGATGGCT CTCGGGACCC AAGAGAGACG 
CTGTCCAAAA GCCAGGACGC GGACGTGCCG GGCGGGCCTG AGAGCCTGAC CTTCCGGGAG 
CGCCAGCGCC TCTTCTCCCA GGGCCACGAG GTCTCCAGTA AGGTGAAAGC CTCCCGCCGG 
CTGACGGAGC TGGAGAACGA GCTGAACACC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839719.1
CDS	22..5517
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984265.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984265.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGATGCGGCT GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG CAGACTGCTG TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGCAGCGG 
GAGGCGGAGG AGCGTGCCCG CCACGAGGAG GAGCGGCGGC GCCAGGAGGA GGAGCGGCTG 
AAGCGCGAGG CCGAGGAAAA GAGGCGCCAG GAGGAGGGGT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCA GACAGCACGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CCCCGCTCCC GCGGGGCTAC GAGCCCCCCG GCCCCGCGCC GCCGCCCCCG 
CCCCAGCGCG CCGCCTCCTA CCTGCAGGCC CAGGTCCTGT CCCCCGACTC CCTGTACACC 
GCCAAGTTCG TGGCTTACGA CGACGATGAC GAGGAGGAGG ACGGCCCTGC AGGTCAGGAT 
AAGTACGCCC GCGCCAGGGG GGCCCGCGGG GGCCCGCGCC CGGCCTCCGA TGGCATCTTT 
CTCGGCAGCT CCGCCCAGGC GCCCTGGGCC AGGGCCAACA GTCCTGCGCA CAGGGCAGGC 
GCGCCCCCGG CCCCGCCGCC CAAGCCCGGC CCCGCCCGAG GCCGAGGGCC CGCCTCGTAC 
ATGGGATCTA CGGGGACGCT GATGGGCGCC CACGATGGCT CTCGGGACCC AAGAGAGACG 
CTGTCCAAAA GCCAGGACGC GGACGTGCCG GGCGGGCCTG AGAGCCTGAC CTTCCGGGAG 
CGCCAGCGCC TCTTCTCCCA GGGCCACGAG GTCTCCAGTA AGGTGAAAGC CTCCCGCCGG 
CTGACGGAGC TGGAGAACGA GCTGAACACC AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984267.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839721.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.

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CloneID	OSe30606
Clone ID Related Accession (Same CDS sequence)	XM_013984267.1
Accession Version	XM_013984267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGATGCGGCT GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCGCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839721.1
CDS	22..5514
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984267.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984267.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGATGCGGCT GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCGCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984272.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839726.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.

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CloneID	OSe30607
Clone ID Related Accession (Same CDS sequence)	XM_013984272.1
Accession Version	XM_013984272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GGAAGTAGAC 
CAGGTGGCTG AGAAACAGAA GAACATCGCG GGGGCGCTTG CGTTCTGGAT GGCCAACGCG 
TCGGAACTCC TCAACTTCAT CAAGCAGGAC CGAGACCTCA GTCGAATCAC ACTGGACGCC 
CAGGATGTTT TGGCTCACCT GGTTCAGATG GCCTTTAAAT ACCTGGTTCA CTGTCTTCAG 
TCAGAGCTGA ACAACTACAT GCCGGCCTTT CTGGACGACC CCGAGGAGAA CAGTCTGCAA 
AGACCAAAAA TAGAGGACGT CCTCCACACG CTCACGGGCG CCATGTCGCT GCTGCGGCGC 
TGCCGGGTCA ACGCCGCCCT CACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCATCAAC 
ATGTGGCTGT TCAACAGGCT GCTGACCGAC CCCGAGTCGG GCCTGTGCTC CCACTACTGG 
GGCGCCATCG TCCGCCAGCA GCTGGGCCAC GTGGAGGCCT GGGCGGAGAA GCAGGGCCTG 
GAGCTGGCTG CCGACTGCCA CCTCAGCAGG GTCGTGCAGG CGACCACCCT GCTCACGATG 
GACAAGTACG CCCCGGAGGA TGCGGCTGGC ATCAACAGCA CCTGCTTCAA GCTGAACTCG 
CTGCAGCTGC AGGCCTTGCT GCACAACTAC CACTGTGCGC CCGACGAGCC CCTGGTCCCG 
CCGGATCTGA TAGAAAGCGT GGTGGCCGTG GCTGAAAACA CGGCGGACGA GCTGGCGCGC 
AGCGACGGCA GGGAGGTGCG GCTGGAGGAG GACCCTGACC TGCAGCTGCC CTTCCTGCTG 
CCGGAGGACG GCTACTCGTG TGACGTCGTC AGAAACATCC CCAACGGCTT GCAGGAGTTT 
TTGGATCCTT TGTGCCAGAG AGGGTTTTGC AGGCTGACGC CCCAGCCGCG CTCTCCGGGT 
TCCTGGACGA TACATTTTGA AGGTGCGGAG TACGAGAGCC GCCTCGCACG GGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCC GAAGTCATCA CTGTGACCCT GAAGAAGCAG 
AACGGGATGG GCCTCAGCAT CGTCGCAGCG AAGGGCGCAG GTCAAGACAA ACTCGGGATC 
TACGTCAAGT CGGTGGTGAG AGGAGGAGCT GCAGACGTGG ACGGGCGGCT GGCGGCGGGG 
GACCAGCTCC TCAGTGTGGA CGGACGAAGC CTGGTGGGCC TCTCTCAGGA AAGGGCAGCA 
GAGCTGATGA CAAGGACAAG TTCCGTGGTG ACCCTGGAGG TAGCGAAGCA GGGAGCCATT 
TACCACGGCC TCGCCACCCT GCTCAACCAG CCCTCGCCCA TGATGCAGAG AATTTCAGAC 
CGGCGTGGAT CAGGTAAACC CCGACCAAAG AGCGAAGGTT TTGAGCTCTA TAATAACTCA 
GCTCAGAACG GGTCCCCCGA GAGCCCCCCG CTGCCCTGGG CCGAGTACAG CGAGCCAAAG 
AAGCTGCCTG GCGACGAGAG GCTGACGAAG AACCGCGCCG ACCACCGCTC CAGCCCCAAC 
GTTGCAAATC AGCCTCCTAG TCCTGGTGGG AAAGGTGCAC ATGCCGCTGG AACAGCAGCC 
AAGATCACAT CCGTCTCCAC TGGCAACCTC TGCACCGAGG AGCAGACGCC CCCGCCGGGG 
ACGGAGGCCT ACCCCATCCC CACGCAGGCC TACCCCCGAG AGTACTTCAC CTTCCCGGCC 
TCCAGGTCCC AGGACCGCGT GGTCCCCCCC CAGAGCCAGT GGCCAAGCTA CGAGGAGAAG 
CCGCACGCGG ACAGCAGCCA CTCCAGCCTC GCGGTTCAGC GGGTGACCCG CTCCCAGGAG 
GAGCTGCGGG AGGACGCGGG GGCCTACCCA CTGCACAGGA TGGAGGCGGT GATGGACCGC 
AAGTCTGACA GCGACATGTG GGTCGCCCAG AGCTCCTCGA TGGGCTCCAG CGCGTCCAGC 
CAGGAGCACC TGGACCCCTC TTCCAAGGCG GGCACCCCCG CGTCCACCCT CACCAAGAGC 
GGCCCGGGGC GCTGGAAGAC CCCTGCAGTT CTGCAGCCCG CGCCCGGCCC CCAGCCGCTC 
CGGACCGACC TGCCCACGCA CTACGCCGGC GACGTGGACG GCGTCCCCGT GGACCTGCCC 
CTCCCGCCGC CTCCGGCTGC CGCGGGCCAG GGACTCGCGG GGGGCCAAGG GGCCGCCGAG 
CGGAAGAAGC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCGCG CCTGGAGGAG 
GAGCGTGAGC GCAAGCGGCG CGAGCAGGAG CGCAAGCTGG GCCAGATGCG CTCGCAGGCG 
CTGCTCCCCG CCCCGCTCTC CCCCGGCGCC ACCAAGCCCG CCGCCCCGGG GCCCCAGGAC 
ACCGTCATCC GGGAGCTGCA GCCTCAGCAG CAGCCGCGCA CCATCGAGCG CAGGGACCTG 
CAGTACATCA CCGTCAGCCG GGAGGAGCTC TCGGCCGGCG ACAGCCTGTC CCCCGACCCC 
TGGAAGCGCG ACGCGCGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACGT GGTGGACCTG 
CTGAGCCGGG AGATTCACGA GCTGCAGAGC AAGGCCGAGC GCTCGGCGGA GGAGAGCGGC 
CGCCTGCGCA AGCTCATGCT GGAGTGGCAG TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG 
AAGGACGAGG ACGACGACGA GGAGGAGGAC GACGACGTGG ACACCATGCT GATCATGCAG 
CGCCTGGAGG CCGAGCGCAG AGCGCGGCAG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCGCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839726.1
CDS	22..5514
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984272.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984272.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GGAAGTAGAC 
CAGGTGGCTG AGAAACAGAA GAACATCGCG GGGGCGCTTG CGTTCTGGAT GGCCAACGCG 
TCGGAACTCC TCAACTTCAT CAAGCAGGAC CGAGACCTCA GTCGAATCAC ACTGGACGCC 
CAGGATGTTT TGGCTCACCT GGTTCAGATG GCCTTTAAAT ACCTGGTTCA CTGTCTTCAG 
TCAGAGCTGA ACAACTACAT GCCGGCCTTT CTGGACGACC CCGAGGAGAA CAGTCTGCAA 
AGACCAAAAA TAGAGGACGT CCTCCACACG CTCACGGGCG CCATGTCGCT GCTGCGGCGC 
TGCCGGGTCA ACGCCGCCCT CACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCATCAAC 
ATGTGGCTGT TCAACAGGCT GCTGACCGAC CCCGAGTCGG GCCTGTGCTC CCACTACTGG 
GGCGCCATCG TCCGCCAGCA GCTGGGCCAC GTGGAGGCCT GGGCGGAGAA GCAGGGCCTG 
GAGCTGGCTG CCGACTGCCA CCTCAGCAGG GTCGTGCAGG CGACCACCCT GCTCACGATG 
GACAAGTACG CCCCGGAGGA TGCGGCTGGC ATCAACAGCA CCTGCTTCAA GCTGAACTCG 
CTGCAGCTGC AGGCCTTGCT GCACAACTAC CACTGTGCGC CCGACGAGCC CCTGGTCCCG 
CCGGATCTGA TAGAAAGCGT GGTGGCCGTG GCTGAAAACA CGGCGGACGA GCTGGCGCGC 
AGCGACGGCA GGGAGGTGCG GCTGGAGGAG GACCCTGACC TGCAGCTGCC CTTCCTGCTG 
CCGGAGGACG GCTACTCGTG TGACGTCGTC AGAAACATCC CCAACGGCTT GCAGGAGTTT 
TTGGATCCTT TGTGCCAGAG AGGGTTTTGC AGGCTGACGC CCCAGCCGCG CTCTCCGGGT 
TCCTGGACGA TACATTTTGA AGGTGCGGAG TACGAGAGCC GCCTCGCACG GGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCC GAAGTCATCA CTGTGACCCT GAAGAAGCAG 
AACGGGATGG GCCTCAGCAT CGTCGCAGCG AAGGGCGCAG GTCAAGACAA ACTCGGGATC 
TACGTCAAGT CGGTGGTGAG AGGAGGAGCT GCAGACGTGG ACGGGCGGCT GGCGGCGGGG 
GACCAGCTCC TCAGTGTGGA CGGACGAAGC CTGGTGGGCC TCTCTCAGGA AAGGGCAGCA 
GAGCTGATGA CAAGGACAAG TTCCGTGGTG ACCCTGGAGG TAGCGAAGCA GGGAGCCATT 
TACCACGGCC TCGCCACCCT GCTCAACCAG CCCTCGCCCA TGATGCAGAG AATTTCAGAC 
CGGCGTGGAT CAGGTAAACC CCGACCAAAG AGCGAAGGTT TTGAGCTCTA TAATAACTCA 
GCTCAGAACG GGTCCCCCGA GAGCCCCCCG CTGCCCTGGG CCGAGTACAG CGAGCCAAAG 
AAGCTGCCTG GCGACGAGAG GCTGACGAAG AACCGCGCCG ACCACCGCTC CAGCCCCAAC 
GTTGCAAATC AGCCTCCTAG TCCTGGTGGG AAAGGTGCAC ATGCCGCTGG AACAGCAGCC 
AAGATCACAT CCGTCTCCAC TGGCAACCTC TGCACCGAGG AGCAGACGCC CCCGCCGGGG 
ACGGAGGCCT ACCCCATCCC CACGCAGGCC TACCCCCGAG AGTACTTCAC CTTCCCGGCC 
TCCAGGTCCC AGGACCGCGT GGTCCCCCCC CAGAGCCAGT GGCCAAGCTA CGAGGAGAAG 
CCGCACGCGG ACAGCAGCCA CTCCAGCCTC GCGGTTCAGC GGGTGACCCG CTCCCAGGAG 
GAGCTGCGGG AGGACGCGGG GGCCTACCCA CTGCACAGGA TGGAGGCGGT GATGGACCGC 
AAGTCTGACA GCGACATGTG GGTCGCCCAG AGCTCCTCGA TGGGCTCCAG CGCGTCCAGC 
CAGGAGCACC TGGACCCCTC TTCCAAGGCG GGCACCCCCG CGTCCACCCT CACCAAGAGC 
GGCCCGGGGC GCTGGAAGAC CCCTGCAGTT CTGCAGCCCG CGCCCGGCCC CCAGCCGCTC 
CGGACCGACC TGCCCACGCA CTACGCCGGC GACGTGGACG GCGTCCCCGT GGACCTGCCC 
CTCCCGCCGC CTCCGGCTGC CGCGGGCCAG GGACTCGCGG GGGGCCAAGG GGCCGCCGAG 
CGGAAGAAGC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCGCG CCTGGAGGAG 
GAGCGTGAGC GCAAGCGGCG CGAGCAGGAG CGCAAGCTGG GCCAGATGCG CTCGCAGGCG 
CTGCTCCCCG CCCCGCTCTC CCCCGGCGCC ACCAAGCCCG CCGCCCCGGG GCCCCAGGAC 
ACCGTCATCC GGGAGCTGCA GCCTCAGCAG CAGCCGCGCA CCATCGAGCG CAGGGACCTG 
CAGTACATCA CCGTCAGCCG GGAGGAGCTC TCGGCCGGCG ACAGCCTGTC CCCCGACCCC 
TGGAAGCGCG ACGCGCGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACGT GGTGGACCTG 
CTGAGCCGGG AGATTCACGA GCTGCAGAGC AAGGCCGAGC GCTCGGCGGA GGAGAGCGGC 
CGCCTGCGCA AGCTCATGCT GGAGTGGCAG TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG 
AAGGACGAGG ACGACGACGA GGAGGAGGAC GACGACGTGG ACACCATGCT GATCATGCAG 
CGCCTGGAGG CCGAGCGCAG AGCGCGGCAG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCGCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984277.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839731.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.

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CloneID	OSe30608
Clone ID Related Accession (Same CDS sequence)	XM_013984277.1
Accession Version	XM_013984277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GGAAGTAGAC 
CAGGTGGCTG AGAAACAGAA GAACATCGCG GGGGCGCTTG CGTTCTGGAT GGCCAACGCG 
TCGGAACTCC TCAACTTCAT CAAGCAGGAC CGAGACCTCA GTCGAATCAC ACTGGACGCC 
CAGGATGTTT TGGCTCACCT GGTTCAGATG GCCTTTAAAT ACCTGGTTCA CTGTCTTCAG 
TCAGAGCTGA ACAACTACAT GCCGGCCTTT CTGGACGACC CCGAGGAGAA CAGTCTGCAA 
AGACCAAAAA TAGAGGACGT CCTCCACACG CTCACGGGCG CCATGTCGCT GCTGCGGCGC 
TGCCGGGTCA ACGCCGCCCT CACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCATCAAC 
ATGTGGCTGT TCAACAGGCT GCTGACCGAC CCCGAGTCGG GCCTGTGCTC CCACTACTGG 
GGCGCCATCG TCCGCCAGCA GCTGGGCCAC GTGGAGGCCT GGGCGGAGAA GCAGGGCCTG 
GAGCTGGCTG CCGACTGCCA CCTCAGCAGG GTCGTGCAGG CGACCACCCT GCTCACGATG 
GACAAGTACG CCCCGGAGGA TGCGGCTGGC ATCAACAGCA CCTGCTTCAA GCTGAACTCG 
CTGCAGCTGC AGGCCTTGCT GCACAACTAC CACTGTGCGC CCGACGAGCC CCTGGTCCCG 
CCGGATCTGA TAGAAAGCGT GGTGGCCGTG GCTGAAAACA CGGCGGACGA GCTGGCGCGC 
AGCGACGGCA GGGAGGTGCG GCTGGAGGAG GACCCTGACC TGCAGCTGCC CTTCCTGCTG 
CCGGAGGACG GCTACTCGTG TGACGTCGTC AGAAACATCC CCAACGGCTT GCAGGAGTTT 
TTGGATCCTT TGTGCCAGAG AGGGTTTTGC AGGCTGACGC CCCAGCCGCG CTCTCCGGGT 
TCCTGGACGA TACATTTTGA AGGTGCGGAG TACGAGAGCC GCCTCGCACG GGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCC GAAGTCATCA CTGTGACCCT GAAGAAGCAG 
AACGGGATGG GCCTCAGCAT CGTCGCAGCG AAGGGCGCAG GTCAAGACAA ACTCGGGATC 
TACGTCAAGT CGGTGGTGAG AGGAGGAGCT GCAGACGTGG ACGGGCGGCT GGCGGCGGGG 
GACCAGCTCC TCAGTGTGGA CGGACGAAGC CTGGTGGGCC TCTCTCAGGA AAGGGCAGCA 
GAGCTGATGA CAAGGACAAG TTCCGTGGTG ACCCTGGAGG TAGCGAAGCA GGGAGCCATT 
TACCACGGCC TCGCCACCCT GCTCAACCAG CCCTCGCCCA TGATGCAGAG AATTTCAGAC 
CGGCGTGGAT CAGGTAAACC CCGACCAAAG AGCGAAGGTT TTGAGCTCTA TAATAACTCA 
GCTCAGAACG GGTCCCCCGA GAGCCCCCCG CTGCCCTGGG CCGAGTACAG CGAGCCAAAG 
AAGCTGCCTG GCGACGAGAG GCTGACGAAG AACCGCGCCG ACCACCGCTC CAGCCCCAAC 
GTTGCAAATC AGCCTCCTAG TCCTGGTGGG AAAGGTGCAC ATGCCGCTGG AACAGCAGCC 
AAGATCACAT CCGTCTCCAC TGGCAACCTC TGCACCGAGG AGCAGACGCC CCCGCCGGGG 
ACGGAGGCCT ACCCCATCCC CACGCAGGCC TACCCCCGAG AGTACTTCAC CTTCCCGGCC 
TCCAGGTCCC AGGACCGCGT GGTCCCCCCC CAGAGCCAGT GGCCAAGCTA CGAGGAGAAG 
CCGCACGCGG ACAGCAGCCA CTCCAGCCTC GCGGTTCAGC GGGTGACCCG CTCCCAGGAG 
GAGCTGCGGG AGGACGCGGG GGCCTACCCA CTGCACAGGA TGGAGGCGGT GATGGACCGC 
AAGTCTGACA GCGACATGTG GGTCGCCCAG AGCTCCTCGA TGGGCTCCAG CGCGTCCAGC 
CAGGAGCACC TGGACCCCTC TTCCAAGGCG GGCACCCCCG CGTCCACCCT CACCAAGAGC 
GGCCCGGGGC GCTGGAAGAC CCCTGCAGTT CTGCAGCCCG CGCCCGGCCC CCAGCCGCTC 
CGGACCGACC TGCCCACGCA CTACGCCGGC GACGTGGACG GCGTCCCCGT GGACCTGCCC 
CTCCCGCCGC CTCCGGCTGC CGCGGGCCAG GGACTCGCGG GGGGCCAAGG GGCCGCCGAG 
CGGAAGAAGC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCGCG CCTGGAGGAG 
GAGCGTGAGC GCAAGCGGCG CGAGCAGGAG CGCAAGCTGG GCCAGATGCG CTCGCAGGCG 
CTGCTCCCCG CCCCGCTCTC CCCCGGCGCC ACCAAGCCCG CCGCCCCGGG GCCCCAGGAC 
ACCGTCATCC GGGAGCTGCA GCCTCAGCAG CAGCCGCGCA CCATCGAGCG CAGGGACCTG 
CAGTACATCA CCGTCAGCCG GGAGGAGCTC TCGGCCGGCG ACAGCCTGTC CCCCGACCCC 
TGGAAGCGCG ACGCGCGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACGT GGTGGACCTG 
CTGAGCCGGG AGATTCACGA GCTGCAGAGC AAGGCCGAGC GCTCGGCGGA GGAGAGCGGC 
CGCCTGCGCA AGCTCATGCT GGAGTGGCAG TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG 
AAGGACGAGG ACGACGACGA GGAGGAGGAC GACGACGTGG ACACCATGCT GATCATGCAG 
CGCCTGGAGG CCGAGCGCAG AGCGCGGCAG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCGCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839731.1
CDS	22..5514
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984277.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984277.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GGAAGTAGAC 
CAGGTGGCTG AGAAACAGAA GAACATCGCG GGGGCGCTTG CGTTCTGGAT GGCCAACGCG 
TCGGAACTCC TCAACTTCAT CAAGCAGGAC CGAGACCTCA GTCGAATCAC ACTGGACGCC 
CAGGATGTTT TGGCTCACCT GGTTCAGATG GCCTTTAAAT ACCTGGTTCA CTGTCTTCAG 
TCAGAGCTGA ACAACTACAT GCCGGCCTTT CTGGACGACC CCGAGGAGAA CAGTCTGCAA 
AGACCAAAAA TAGAGGACGT CCTCCACACG CTCACGGGCG CCATGTCGCT GCTGCGGCGC 
TGCCGGGTCA ACGCCGCCCT CACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCATCAAC 
ATGTGGCTGT TCAACAGGCT GCTGACCGAC CCCGAGTCGG GCCTGTGCTC CCACTACTGG 
GGCGCCATCG TCCGCCAGCA GCTGGGCCAC GTGGAGGCCT GGGCGGAGAA GCAGGGCCTG 
GAGCTGGCTG CCGACTGCCA CCTCAGCAGG GTCGTGCAGG CGACCACCCT GCTCACGATG 
GACAAGTACG CCCCGGAGGA TGCGGCTGGC ATCAACAGCA CCTGCTTCAA GCTGAACTCG 
CTGCAGCTGC AGGCCTTGCT GCACAACTAC CACTGTGCGC CCGACGAGCC CCTGGTCCCG 
CCGGATCTGA TAGAAAGCGT GGTGGCCGTG GCTGAAAACA CGGCGGACGA GCTGGCGCGC 
AGCGACGGCA GGGAGGTGCG GCTGGAGGAG GACCCTGACC TGCAGCTGCC CTTCCTGCTG 
CCGGAGGACG GCTACTCGTG TGACGTCGTC AGAAACATCC CCAACGGCTT GCAGGAGTTT 
TTGGATCCTT TGTGCCAGAG AGGGTTTTGC AGGCTGACGC CCCAGCCGCG CTCTCCGGGT 
TCCTGGACGA TACATTTTGA AGGTGCGGAG TACGAGAGCC GCCTCGCACG GGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCC GAAGTCATCA CTGTGACCCT GAAGAAGCAG 
AACGGGATGG GCCTCAGCAT CGTCGCAGCG AAGGGCGCAG GTCAAGACAA ACTCGGGATC 
TACGTCAAGT CGGTGGTGAG AGGAGGAGCT GCAGACGTGG ACGGGCGGCT GGCGGCGGGG 
GACCAGCTCC TCAGTGTGGA CGGACGAAGC CTGGTGGGCC TCTCTCAGGA AAGGGCAGCA 
GAGCTGATGA CAAGGACAAG TTCCGTGGTG ACCCTGGAGG TAGCGAAGCA GGGAGCCATT 
TACCACGGCC TCGCCACCCT GCTCAACCAG CCCTCGCCCA TGATGCAGAG AATTTCAGAC 
CGGCGTGGAT CAGGTAAACC CCGACCAAAG AGCGAAGGTT TTGAGCTCTA TAATAACTCA 
GCTCAGAACG GGTCCCCCGA GAGCCCCCCG CTGCCCTGGG CCGAGTACAG CGAGCCAAAG 
AAGCTGCCTG GCGACGAGAG GCTGACGAAG AACCGCGCCG ACCACCGCTC CAGCCCCAAC 
GTTGCAAATC AGCCTCCTAG TCCTGGTGGG AAAGGTGCAC ATGCCGCTGG AACAGCAGCC 
AAGATCACAT CCGTCTCCAC TGGCAACCTC TGCACCGAGG AGCAGACGCC CCCGCCGGGG 
ACGGAGGCCT ACCCCATCCC CACGCAGGCC TACCCCCGAG AGTACTTCAC CTTCCCGGCC 
TCCAGGTCCC AGGACCGCGT GGTCCCCCCC CAGAGCCAGT GGCCAAGCTA CGAGGAGAAG 
CCGCACGCGG ACAGCAGCCA CTCCAGCCTC GCGGTTCAGC GGGTGACCCG CTCCCAGGAG 
GAGCTGCGGG AGGACGCGGG GGCCTACCCA CTGCACAGGA TGGAGGCGGT GATGGACCGC 
AAGTCTGACA GCGACATGTG GGTCGCCCAG AGCTCCTCGA TGGGCTCCAG CGCGTCCAGC 
CAGGAGCACC TGGACCCCTC TTCCAAGGCG GGCACCCCCG CGTCCACCCT CACCAAGAGC 
GGCCCGGGGC GCTGGAAGAC CCCTGCAGTT CTGCAGCCCG CGCCCGGCCC CCAGCCGCTC 
CGGACCGACC TGCCCACGCA CTACGCCGGC GACGTGGACG GCGTCCCCGT GGACCTGCCC 
CTCCCGCCGC CTCCGGCTGC CGCGGGCCAG GGACTCGCGG GGGGCCAAGG GGCCGCCGAG 
CGGAAGAAGC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCGCG CCTGGAGGAG 
GAGCGTGAGC GCAAGCGGCG CGAGCAGGAG CGCAAGCTGG GCCAGATGCG CTCGCAGGCG 
CTGCTCCCCG CCCCGCTCTC CCCCGGCGCC ACCAAGCCCG CCGCCCCGGG GCCCCAGGAC 
ACCGTCATCC GGGAGCTGCA GCCTCAGCAG CAGCCGCGCA CCATCGAGCG CAGGGACCTG 
CAGTACATCA CCGTCAGCCG GGAGGAGCTC TCGGCCGGCG ACAGCCTGTC CCCCGACCCC 
TGGAAGCGCG ACGCGCGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACGT GGTGGACCTG 
CTGAGCCGGG AGATTCACGA GCTGCAGAGC AAGGCCGAGC GCTCGGCGGA GGAGAGCGGC 
CGCCTGCGCA AGCTCATGCT GGAGTGGCAG TTCCAGAAGC GGCTGCAGGA GTCCAAGCAG 
AAGGACGAGG ACGACGACGA GGAGGAGGAC GACGACGTGG ACACCATGCT GATCATGCAG 
CGCCTGGAGG CCGAGCGCAG AGCGCGGCAG ACTGCTGTGC CAGCAATCTC TGTTCTAGAT 
TTGTTGCAGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG TCAGGATAAG 
TACGCCCGCG CCAGGGGGGC CCGCGGGGGC CCGCGCCCGG CCTCCGATGG CATCTTTCTC 
GGCAGCTCCG CCCAGGCGCC CTGGGCCAGG GCCAACAGTC CTGCGCACAG GGCAGGCGCG 
CCCCCGGCCC CGCCGCCCAA GCCCGGCCCC GCCCGAGGCC GAGGGCCCGC CTCGTACATG 
GGATCTACGG GGACGCTGAT GGGCGCCCAC GATGGCTCTC GGGACCCAAG AGAGACGCTG 
TCCAAAAGCC AGGACGCGGA CGTGCCGGGC GGGCCTGAGA GCCTGACCTT CCGGGAGCGC 
CAGCGCCTCT TCTCCCAGGG CCACGAGGTC TCCAGTAAGG TGAAAGCCTC CCGCCGGCTG 
ACGGAGCTGG AGAACGAGCT GAACACCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984278.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839732.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.

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CloneID	OSe30609
Clone ID Related Accession (Same CDS sequence)	XM_013984278.1
Accession Version	XM_013984278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGAAACAG 
AAGAACATCG CGGGGGCGCT TGCGTTCTGG ATGGCCAACG CGTCGGAACT CCTCAACTTC 
ATCAAGCAGG ACCGAGACCT CAGTCGAATC ACACTGGACG CCCAGGATGT TTTGGCTCAC 
CTGGTTCAGA TGGCCTTTAA ATACCTGGTT CACTGTCTTC AGTCAGAGCT GAACAACTAC 
ATGCCGGCCT TTCTGGACGA CCCCGAGGAG AACAGTCTGC AAAGACCAAA AATAGAGGAC 
GTCCTCCACA CGCTCACGGG CGCCATGTCG CTGCTGCGGC GCTGCCGGGT CAACGCCGCC 
CTCACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCATCA ACATGTGGCT GTTCAACAGG 
CTGCTGACCG ACCCCGAGTC GGGCCTGTGC TCCCACTACT GGGGCGCCAT CGTCCGCCAG 
CAGCTGGGCC ACGTGGAGGC CTGGGCGGAG AAGCAGGGCC TGGAGCTGGC TGCCGACTGC 
CACCTCAGCA GGGTCGTGCA GGCGACCACC CTGCTCACGA TGGACAAGTA CGCCCCGGAG 
GATGCGGCTG GCATCAACAG CACCTGCTTC AAGCTGAACT CGCTGCAGCT GCAGGCCTTG 
CTGCACAACT ACCACTGTGC GCCCGACGAG CCCCTGGTCC CGCCGGATCT GATAGAAAGC 
GTGGTGGCCG TGGCTGAAAA CACGGCGGAC GAGCTGGCGC GCAGCGACGG CAGGGAGGTG 
CGGCTGGAGG AGGACCCTGA CCTGCAGCTG CCCTTCCTGC TGCCGGAGGA CGGCTACTCG 
TGTGACGTCG TCAGAAACAT CCCCAACGGC TTGCAGGAGT TTTTGGATCC TTTGTGCCAG 
AGAGGGTTTT GCAGGCTGAC GCCCCAGCCG CGCTCTCCGG GTTCCTGGAC GATACATTTT 
GAAGGTGCGG AGTACGAGAG CCGCCTCGCA CGGGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CCGAAGTCAT CACTGTGACC CTGAAGAAGC AGAACGGGAT GGGCCTCAGC 
ATCGTCGCAG CGAAGGGCGC AGGTCAAGAC AAACTCGGGA TCTACGTCAA GTCGGTGGTG 
AGAGGAGGAG CTGCAGACGT GGACGGGCGG CTGGCGGCGG GGGACCAGCT CCTCAGTGTG 
GACGGACGAA GCCTGGTGGG CCTCTCTCAG GAAAGGGCAG CAGAGCTGAT GACAAGGACA 
AGTTCCGTGG TGACCCTGGA GGTAGCGAAG CAGGGAGCCA TTTACCACGG CCTCGCCACC 
CTGCTCAACC AGCCCTCGCC CATGATGCAG AGAATTTCAG ACCGGCGTGG ATCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTTGAGCTC TATAATAACT CAGCTCAGAA CGGGTCCCCC 
GAGAGCCCCC CGCTGCCCTG GGCCGAGTAC AGCGAGCCAA AGAAGCTGCC TGGCGACGAG 
AGGCTGACGA AGAACCGCGC CGACCACCGC TCCAGCCCCA ACGTTGCAAA TCAGCCTCCT 
AGTCCTGGTG GGAAAGGTGC ACATGCCGCT GGAACAGCAG CCAAGATCAC ATCCGTCTCC 
ACTGGCAACC TCTGCACCGA GGAGCAGACG CCCCCGCCGG GGACGGAGGC CTACCCCATC 
CCCACGCAGG CCTACCCCCG AGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGTCCCCC CCCAGAGCCA GTGGCCAAGC TACGAGGAGA AGCCGCACGC GGACAGCAGC 
CACTCCAGCC TCGCGGTTCA GCGGGTGACC CGCTCCCAGG AGGAGCTGCG GGAGGACGCG 
GGGGCCTACC CACTGCACAG GATGGAGGCG GTGATGGACC GCAAGTCTGA CAGCGACATG 
TGGGTCGCCC AGAGCTCCTC GATGGGCTCC AGCGCGTCCA GCCAGGAGCA CCTGGACCCC 
TCTTCCAAGG CGGGCACCCC CGCGTCCACC CTCACCAAGA GCGGCCCGGG GCGCTGGAAG 
ACCCCTGCAG TTCTGCAGCC CGCGCCCGGC CCCCAGCCGC TCCGGACCGA CCTGCCCACG 
CACTACGCCG GCGACGTGGA CGGCGTCCCC GTGGACCTGC CCCTCCCGCC GCCTCCGGCT 
GCCGCGGGCC AGGGACTCGC GGGGGGCCAA GGGGCCGCCG AGCGGAAGAA GCGGGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAGAAGGCG CGCCTGGAGG AGGAGCGTGA GCGCAAGCGG 
CGCGAGCAGG AGCGCAAGCT GGGCCAGATG CGCTCGCAGG CGCTGCTCCC CGCCCCGCTC 
TCCCCCGGCG CCACCAAGCC CGCCGCCCCG GGGCCCCAGG ACACCGTCAT CCGGGAGCTG 
CAGCCTCAGC AGCAGCCGCG CACCATCGAG CGCAGGGACC TGCAGTACAT CACCGTCAGC 
CGGGAGGAGC TCTCGGCCGG CGACAGCCTG TCCCCCGACC CCTGGAAGCG CGACGCGCGG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC GTGGTGGACC TGCTGAGCCG GGAGATTCAC 
GAGCTGCAGA GCAAGGCCGA GCGCTCGGCG GAGGAGAGCG GCCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAC 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTGT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGACGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGCAGCGGG AGGCGGAGGA GCGTGCCCGC 
CACGAGGAGG AGCGGCGGCG CCAGGAGGAG GAGCGGCTGA AGCGCGAGGC CGAGGAAAAG 
AGGCGCCAGG AGGAGGGGTA CTACAGCCGC CTGGAGGCCG AGCGGCGCAG ACAGCACGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC CCCGCTCCCG 
CGGGGCTACG AGCCCCCCGG CCCCGCGCCG CCGCCCCCGC CCCAGCGCGC CGCCTCCTAC 
CTGCAGGCCC AGGTCCTGTC CCCCGACTCC CTGTACACCG CCAAGTTCGT GGCTTACGAC 
GACGATGACG AGGAGGAGGA CGGCCCTGCA GGTCAGGATA AGTACGCCCG CGCCAGGGGG 
GCCCGCGGGG GCCCGCGCCC GGCCTCCGAT GGCATCTTTC TCGGCAGCTC CGCCCAGGCG 
CCCTGGGCCA GGGCCAACAG TCCTGCGCAC AGGGCAGGCG CGCCCCCGGC CCCGCCGCCC 
AAGCCCGGCC CCGCCCGAGG CCGAGGGCCC GCCTCGTACA TGGGATCTAC GGGGACGCTG 
ATGGGCGCCC ACGATGGCTC TCGGGACCCA AGAGAGACGC TGTCCAAAAG CCAGGACGCG 
GACGTGCCGG GCGGGCCTGA GAGCCTGACC TTCCGGGAGC GCCAGCGCCT CTTCTCCCAG 
GGCCACGAGG TCTCCAGTAA GGTGAAAGCC TCCCGCCGGC TGACGGAGCT GGAGAACGAG 
CTGAACACCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839732.1
CDS	22..5496
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984278.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984278.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGAAACAG 
AAGAACATCG CGGGGGCGCT TGCGTTCTGG ATGGCCAACG CGTCGGAACT CCTCAACTTC 
ATCAAGCAGG ACCGAGACCT CAGTCGAATC ACACTGGACG CCCAGGATGT TTTGGCTCAC 
CTGGTTCAGA TGGCCTTTAA ATACCTGGTT CACTGTCTTC AGTCAGAGCT GAACAACTAC 
ATGCCGGCCT TTCTGGACGA CCCCGAGGAG AACAGTCTGC AAAGACCAAA AATAGAGGAC 
GTCCTCCACA CGCTCACGGG CGCCATGTCG CTGCTGCGGC GCTGCCGGGT CAACGCCGCC 
CTCACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCATCA ACATGTGGCT GTTCAACAGG 
CTGCTGACCG ACCCCGAGTC GGGCCTGTGC TCCCACTACT GGGGCGCCAT CGTCCGCCAG 
CAGCTGGGCC ACGTGGAGGC CTGGGCGGAG AAGCAGGGCC TGGAGCTGGC TGCCGACTGC 
CACCTCAGCA GGGTCGTGCA GGCGACCACC CTGCTCACGA TGGACAAGTA CGCCCCGGAG 
GATGCGGCTG GCATCAACAG CACCTGCTTC AAGCTGAACT CGCTGCAGCT GCAGGCCTTG 
CTGCACAACT ACCACTGTGC GCCCGACGAG CCCCTGGTCC CGCCGGATCT GATAGAAAGC 
GTGGTGGCCG TGGCTGAAAA CACGGCGGAC GAGCTGGCGC GCAGCGACGG CAGGGAGGTG 
CGGCTGGAGG AGGACCCTGA CCTGCAGCTG CCCTTCCTGC TGCCGGAGGA CGGCTACTCG 
TGTGACGTCG TCAGAAACAT CCCCAACGGC TTGCAGGAGT TTTTGGATCC TTTGTGCCAG 
AGAGGGTTTT GCAGGCTGAC GCCCCAGCCG CGCTCTCCGG GTTCCTGGAC GATACATTTT 
GAAGGTGCGG AGTACGAGAG CCGCCTCGCA CGGGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CCGAAGTCAT CACTGTGACC CTGAAGAAGC AGAACGGGAT GGGCCTCAGC 
ATCGTCGCAG CGAAGGGCGC AGGTCAAGAC AAACTCGGGA TCTACGTCAA GTCGGTGGTG 
AGAGGAGGAG CTGCAGACGT GGACGGGCGG CTGGCGGCGG GGGACCAGCT CCTCAGTGTG 
GACGGACGAA GCCTGGTGGG CCTCTCTCAG GAAAGGGCAG CAGAGCTGAT GACAAGGACA 
AGTTCCGTGG TGACCCTGGA GGTAGCGAAG CAGGGAGCCA TTTACCACGG CCTCGCCACC 
CTGCTCAACC AGCCCTCGCC CATGATGCAG AGAATTTCAG ACCGGCGTGG ATCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTTGAGCTC TATAATAACT CAGCTCAGAA CGGGTCCCCC 
GAGAGCCCCC CGCTGCCCTG GGCCGAGTAC AGCGAGCCAA AGAAGCTGCC TGGCGACGAG 
AGGCTGACGA AGAACCGCGC CGACCACCGC TCCAGCCCCA ACGTTGCAAA TCAGCCTCCT 
AGTCCTGGTG GGAAAGGTGC ACATGCCGCT GGAACAGCAG CCAAGATCAC ATCCGTCTCC 
ACTGGCAACC TCTGCACCGA GGAGCAGACG CCCCCGCCGG GGACGGAGGC CTACCCCATC 
CCCACGCAGG CCTACCCCCG AGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGTCCCCC CCCAGAGCCA GTGGCCAAGC TACGAGGAGA AGCCGCACGC GGACAGCAGC 
CACTCCAGCC TCGCGGTTCA GCGGGTGACC CGCTCCCAGG AGGAGCTGCG GGAGGACGCG 
GGGGCCTACC CACTGCACAG GATGGAGGCG GTGATGGACC GCAAGTCTGA CAGCGACATG 
TGGGTCGCCC AGAGCTCCTC GATGGGCTCC AGCGCGTCCA GCCAGGAGCA CCTGGACCCC 
TCTTCCAAGG CGGGCACCCC CGCGTCCACC CTCACCAAGA GCGGCCCGGG GCGCTGGAAG 
ACCCCTGCAG TTCTGCAGCC CGCGCCCGGC CCCCAGCCGC TCCGGACCGA CCTGCCCACG 
CACTACGCCG GCGACGTGGA CGGCGTCCCC GTGGACCTGC CCCTCCCGCC GCCTCCGGCT 
GCCGCGGGCC AGGGACTCGC GGGGGGCCAA GGGGCCGCCG AGCGGAAGAA GCGGGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAGAAGGCG CGCCTGGAGG AGGAGCGTGA GCGCAAGCGG 
CGCGAGCAGG AGCGCAAGCT GGGCCAGATG CGCTCGCAGG CGCTGCTCCC CGCCCCGCTC 
TCCCCCGGCG CCACCAAGCC CGCCGCCCCG GGGCCCCAGG ACACCGTCAT CCGGGAGCTG 
CAGCCTCAGC AGCAGCCGCG CACCATCGAG CGCAGGGACC TGCAGTACAT CACCGTCAGC 
CGGGAGGAGC TCTCGGCCGG CGACAGCCTG TCCCCCGACC CCTGGAAGCG CGACGCGCGG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC GTGGTGGACC TGCTGAGCCG GGAGATTCAC 
GAGCTGCAGA GCAAGGCCGA GCGCTCGGCG GAGGAGAGCG GCCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAC 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGC AGACTGCTGT GCCAGCAATC TCTGTTCTAG ATTTGTTGCA GGACGAGGAG 
CGGCGGCGGC AGCAGCAGCT GGAGGAGATG CGGCAGCGGG AGGCGGAGGA GCGTGCCCGC 
CACGAGGAGG AGCGGCGGCG CCAGGAGGAG GAGCGGCTGA AGCGCGAGGC CGAGGAAAAG 
AGGCGCCAGG AGGAGGGGTA CTACAGCCGC CTGGAGGCCG AGCGGCGCAG ACAGCACGAG 
GAGGCCGAGC GCCGGCTGCT GGAGCCCGAG GAGCCGGGCC TGGGCCGCCC CCCGCTCCCG 
CGGGGCTACG AGCCCCCCGG CCCCGCGCCG CCGCCCCCGC CCCAGCGCGC CGCCTCCTAC 
CTGCAGGCCC AGGTCCTGTC CCCCGACTCC CTGTACACCG CCAAGTTCGT GGCTTACGAC 
GACGATGACG AGGAGGAGGA CGGCCCTGCA GGTCAGGATA AGTACGCCCG CGCCAGGGGG 
GCCCGCGGGG GCCCGCGCCC GGCCTCCGAT GGCATCTTTC TCGGCAGCTC CGCCCAGGCG 
CCCTGGGCCA GGGCCAACAG TCCTGCGCAC AGGGCAGGCG CGCCCCCGGC CCCGCCGCCC 
AAGCCCGGCC CCGCCCGAGG CCGAGGGCCC GCCTCGTACA TGGGATCTAC GGGGACGCTG 
ATGGGCGCCC ACGATGGCTC TCGGGACCCA AGAGAGACGC TGTCCAAAAG CCAGGACGCG 
GACGTGCCGG GCGGGCCTGA GAGCCTGACC TTCCGGGAGC GCCAGCGCCT CTTCTCCCAG 
GGCCACGAGG TCTCCAGTAA GGTGAAAGCC TCCCGCCGGC TGACGGAGCT GGAGAACGAG 
CTGAACACCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984282.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839736.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

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CloneID	OSe30610
Clone ID Related Accession (Same CDS sequence)	XM_013984282.1
Accession Version	XM_013984282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GAAACAGAAG 
AACATCGCGG GGGCGCTTGC GTTCTGGATG GCCAACGCGT CGGAACTCCT CAACTTCATC 
AAGCAGGACC GAGACCTCAG TCGAATCACA CTGGACGCCC AGGATGTTTT GGCTCACCTG 
GTTCAGATGG CCTTTAAATA CCTGGTTCAC TGTCTTCAGT CAGAGCTGAA CAACTACATG 
CCGGCCTTTC TGGACGACCC CGAGGAGAAC AGTCTGCAAA GACCAAAAAT AGAGGACGTC 
CTCCACACGC TCACGGGCGC CATGTCGCTG CTGCGGCGCT GCCGGGTCAA CGCCGCCCTC 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCATCAACA TGTGGCTGTT CAACAGGCTG 
CTGACCGACC CCGAGTCGGG CCTGTGCTCC CACTACTGGG GCGCCATCGT CCGCCAGCAG 
CTGGGCCACG TGGAGGCCTG GGCGGAGAAG CAGGGCCTGG AGCTGGCTGC CGACTGCCAC 
CTCAGCAGGG TCGTGCAGGC GACCACCCTG CTCACGATGG ACAAGTACGC CCCGGAGGAT 
GCGGCTGGCA TCAACAGCAC CTGCTTCAAG CTGAACTCGC TGCAGCTGCA GGCCTTGCTG 
CACAACTACC ACTGTGCGCC CGACGAGCCC CTGGTCCCGC CGGATCTGAT AGAAAGCGTG 
GTGGCCGTGG CTGAAAACAC GGCGGACGAG CTGGCGCGCA GCGACGGCAG GGAGGTGCGG 
CTGGAGGAGG ACCCTGACCT GCAGCTGCCC TTCCTGCTGC CGGAGGACGG CTACTCGTGT 
GACGTCGTCA GAAACATCCC CAACGGCTTG CAGGAGTTTT TGGATCCTTT GTGCCAGAGA 
GGGTTTTGCA GGCTGACGCC CCAGCCGCGC TCTCCGGGTT CCTGGACGAT ACATTTTGAA 
GGTGCGGAGT ACGAGAGCCG CCTCGCACGG GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCCG AAGTCATCAC TGTGACCCTG AAGAAGCAGA ACGGGATGGG CCTCAGCATC 
GTCGCAGCGA AGGGCGCAGG TCAAGACAAA CTCGGGATCT ACGTCAAGTC GGTGGTGAGA 
GGAGGAGCTG CAGACGTGGA CGGGCGGCTG GCGGCGGGGG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGCC TGGTGGGCCT CTCTCAGGAA AGGGCAGCAG AGCTGATGAC AAGGACAAGT 
TCCGTGGTGA CCCTGGAGGT AGCGAAGCAG GGAGCCATTT ACCACGGCCT CGCCACCCTG 
CTCAACCAGC CCTCGCCCAT GATGCAGAGA ATTTCAGACC GGCGTGGATC AGGTAAACCC 
CGACCAAAGA GCGAAGGTTT TGAGCTCTAT AATAACTCAG CTCAGAACGG GTCCCCCGAG 
AGCCCCCCGC TGCCCTGGGC CGAGTACAGC GAGCCAAAGA AGCTGCCTGG CGACGAGAGG 
CTGACGAAGA ACCGCGCCGA CCACCGCTCC AGCCCCAACG TTGCAAATCA GCCTCCTAGT 
CCTGGTGGGA AAGGTGCACA TGCCGCTGGA ACAGCAGCCA AGATCACATC CGTCTCCACT 
GGCAACCTCT GCACCGAGGA GCAGACGCCC CCGCCGGGGA CGGAGGCCTA CCCCATCCCC 
ACGCAGGCCT ACCCCCGAGA GTACTTCACC TTCCCGGCCT CCAGGTCCCA GGACCGCGTG 
GTCCCCCCCC AGAGCCAGTG GCCAAGCTAC GAGGAGAAGC CGCACGCGGA CAGCAGCCAC 
TCCAGCCTCG CGGTTCAGCG GGTGACCCGC TCCCAGGAGG AGCTGCGGGA GGACGCGGGG 
GCCTACCCAC TGCACAGGAT GGAGGCGGTG ATGGACCGCA AGTCTGACAG CGACATGTGG 
GTCGCCCAGA GCTCCTCGAT GGGCTCCAGC GCGTCCAGCC AGGAGCACCT GGACCCCTCT 
TCCAAGGCGG GCACCCCCGC GTCCACCCTC ACCAAGAGCG GCCCGGGGCG CTGGAAGACC 
CCTGCAGTTC TGCAGCCCGC GCCCGGCCCC CAGCCGCTCC GGACCGACCT GCCCACGCAC 
TACGCCGGCG ACGTGGACGG CGTCCCCGTG GACCTGCCCC TCCCGCCGCC TCCGGCTGCC 
GCGGGCCAGG GACTCGCGGG GGGCCAAGGG GCCGCCGAGC GGAAGAAGCG GGAGGAGCAG 
CAGCGCTGGT ACGAGAAGGA GAAGGCGCGC CTGGAGGAGG AGCGTGAGCG CAAGCGGCGC 
GAGCAGGAGC GCAAGCTGGG CCAGATGCGC TCGCAGGCGC TGCTCCCCGC CCCGCTCTCC 
CCCGGCGCCA CCAAGCCCGC CGCCCCGGGG CCCCAGGACA CCGTCATCCG GGAGCTGCAG 
CCTCAGCAGC AGCCGCGCAC CATCGAGCGC AGGGACCTGC AGTACATCAC CGTCAGCCGG 
GAGGAGCTCT CGGCCGGCGA CAGCCTGTCC CCCGACCCCT GGAAGCGCGA CGCGCGGGAG 
AAGCTGGAGA AGCAGCAGCA GATGCACGTG GTGGACCTGC TGAGCCGGGA GATTCACGAG 
CTGCAGAGCA AGGCCGAGCG CTCGGCGGAG GAGAGCGGCC GCCTGCGCAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGACGAG 
GAGGAGGACG ACGACGTGGA CACCATGCTG ATCATGCAGC GCCTGGAGGC CGAGCGCAGA 
GCGCGGCAGA CTGCTGTGCC AGCAATCTCT GTTCTAGATT TGTTGCAGGA CGAGGAGCGG 
CGGCGGCAGC AGCAGCTGGA GGAGATGCGG CAGCGGGAGG CGGAGGAGCG TGCCCGCCAC 
GAGGAGGAGC GGCGGCGCCA GGAGGAGGAG CGGCTGAAGC GCGAGGCCGA GGAAAAGAGG 
CGCCAGGAGG AGGGGTACTA CAGCCGCCTG GAGGCCGAGC GGCGCAGACA GCACGAGGAG 
GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG CCGGGCCTGG GCCGCCCCCC GCTCCCGCGG 
GGCTACGAGC CCCCCGGCCC CGCGCCGCCG CCCCCGCCCC AGCGCGCCGC CTCCTACCTG 
CAGGCCCAGG TCCTGTCCCC CGACTCCCTG TACACCGCCA AGTTCGTGGC TTACGACGAC 
GATGACGAGG AGGAGGACGG CCCTGCAGGT CAGGATAAGT ACGCCCGCGC CAGGGGGGCC 
CGCGGGGGCC CGCGCCCGGC CTCCGATGGC ATCTTTCTCG GCAGCTCCGC CCAGGCGCCC 
TGGGCCAGGG CCAACAGTCC TGCGCACAGG GCAGGCGCGC CCCCGGCCCC GCCGCCCAAG 
CCCGGCCCCG CCCGAGGCCG AGGGCCCGCC TCGTACATGG GATCTACGGG GACGCTGATG 
GGCGCCCACG ATGGCTCTCG GGACCCAAGA GAGACGCTGT CCAAAAGCCA GGACGCGGAC 
GTGCCGGGCG GGCCTGAGAG CCTGACCTTC CGGGAGCGCC AGCGCCTCTT CTCCCAGGGC 
CACGAGGTCT CCAGTAAGGT GAAAGCCTCC CGCCGGCTGA CGGAGCTGGA GAACGAGCTG 
AACACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839736.1
CDS	22..5493
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984282.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984282.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GAAACAGAAG 
AACATCGCGG GGGCGCTTGC GTTCTGGATG GCCAACGCGT CGGAACTCCT CAACTTCATC 
AAGCAGGACC GAGACCTCAG TCGAATCACA CTGGACGCCC AGGATGTTTT GGCTCACCTG 
GTTCAGATGG CCTTTAAATA CCTGGTTCAC TGTCTTCAGT CAGAGCTGAA CAACTACATG 
CCGGCCTTTC TGGACGACCC CGAGGAGAAC AGTCTGCAAA GACCAAAAAT AGAGGACGTC 
CTCCACACGC TCACGGGCGC CATGTCGCTG CTGCGGCGCT GCCGGGTCAA CGCCGCCCTC 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCATCAACA TGTGGCTGTT CAACAGGCTG 
CTGACCGACC CCGAGTCGGG CCTGTGCTCC CACTACTGGG GCGCCATCGT CCGCCAGCAG 
CTGGGCCACG TGGAGGCCTG GGCGGAGAAG CAGGGCCTGG AGCTGGCTGC CGACTGCCAC 
CTCAGCAGGG TCGTGCAGGC GACCACCCTG CTCACGATGG ACAAGTACGC CCCGGAGGAT 
GCGGCTGGCA TCAACAGCAC CTGCTTCAAG CTGAACTCGC TGCAGCTGCA GGCCTTGCTG 
CACAACTACC ACTGTGCGCC CGACGAGCCC CTGGTCCCGC CGGATCTGAT AGAAAGCGTG 
GTGGCCGTGG CTGAAAACAC GGCGGACGAG CTGGCGCGCA GCGACGGCAG GGAGGTGCGG 
CTGGAGGAGG ACCCTGACCT GCAGCTGCCC TTCCTGCTGC CGGAGGACGG CTACTCGTGT 
GACGTCGTCA GAAACATCCC CAACGGCTTG CAGGAGTTTT TGGATCCTTT GTGCCAGAGA 
GGGTTTTGCA GGCTGACGCC CCAGCCGCGC TCTCCGGGTT CCTGGACGAT ACATTTTGAA 
GGTGCGGAGT ACGAGAGCCG CCTCGCACGG GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCCG AAGTCATCAC TGTGACCCTG AAGAAGCAGA ACGGGATGGG CCTCAGCATC 
GTCGCAGCGA AGGGCGCAGG TCAAGACAAA CTCGGGATCT ACGTCAAGTC GGTGGTGAGA 
GGAGGAGCTG CAGACGTGGA CGGGCGGCTG GCGGCGGGGG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGCC TGGTGGGCCT CTCTCAGGAA AGGGCAGCAG AGCTGATGAC AAGGACAAGT 
TCCGTGGTGA CCCTGGAGGT AGCGAAGCAG GGAGCCATTT ACCACGGCCT CGCCACCCTG 
CTCAACCAGC CCTCGCCCAT GATGCAGAGA ATTTCAGACC GGCGTGGATC AGGTAAACCC 
CGACCAAAGA GCGAAGGTTT TGAGCTCTAT AATAACTCAG CTCAGAACGG GTCCCCCGAG 
AGCCCCCCGC TGCCCTGGGC CGAGTACAGC GAGCCAAAGA AGCTGCCTGG CGACGAGAGG 
CTGACGAAGA ACCGCGCCGA CCACCGCTCC AGCCCCAACG TTGCAAATCA GCCTCCTAGT 
CCTGGTGGGA AAGGTGCACA TGCCGCTGGA ACAGCAGCCA AGATCACATC CGTCTCCACT 
GGCAACCTCT GCACCGAGGA GCAGACGCCC CCGCCGGGGA CGGAGGCCTA CCCCATCCCC 
ACGCAGGCCT ACCCCCGAGA GTACTTCACC TTCCCGGCCT CCAGGTCCCA GGACCGCGTG 
GTCCCCCCCC AGAGCCAGTG GCCAAGCTAC GAGGAGAAGC CGCACGCGGA CAGCAGCCAC 
TCCAGCCTCG CGGTTCAGCG GGTGACCCGC TCCCAGGAGG AGCTGCGGGA GGACGCGGGG 
GCCTACCCAC TGCACAGGAT GGAGGCGGTG ATGGACCGCA AGTCTGACAG CGACATGTGG 
GTCGCCCAGA GCTCCTCGAT GGGCTCCAGC GCGTCCAGCC AGGAGCACCT GGACCCCTCT 
TCCAAGGCGG GCACCCCCGC GTCCACCCTC ACCAAGAGCG GCCCGGGGCG CTGGAAGACC 
CCTGCAGTTC TGCAGCCCGC GCCCGGCCCC CAGCCGCTCC GGACCGACCT GCCCACGCAC 
TACGCCGGCG ACGTGGACGG CGTCCCCGTG GACCTGCCCC TCCCGCCGCC TCCGGCTGCC 
GCGGGCCAGG GACTCGCGGG GGGCCAAGGG GCCGCCGAGC GGAAGAAGCG GGAGGAGCAG 
CAGCGCTGGT ACGAGAAGGA GAAGGCGCGC CTGGAGGAGG AGCGTGAGCG CAAGCGGCGC 
GAGCAGGAGC GCAAGCTGGG CCAGATGCGC TCGCAGGCGC TGCTCCCCGC CCCGCTCTCC 
CCCGGCGCCA CCAAGCCCGC CGCCCCGGGG CCCCAGGACA CCGTCATCCG GGAGCTGCAG 
CCTCAGCAGC AGCCGCGCAC CATCGAGCGC AGGGACCTGC AGTACATCAC CGTCAGCCGG 
GAGGAGCTCT CGGCCGGCGA CAGCCTGTCC CCCGACCCCT GGAAGCGCGA CGCGCGGGAG 
AAGCTGGAGA AGCAGCAGCA GATGCACGTG GTGGACCTGC TGAGCCGGGA GATTCACGAG 
CTGCAGAGCA AGGCCGAGCG CTCGGCGGAG GAGAGCGGCC GCCTGCGCAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGACGAG 
GAGGAGGACG ACGACGTGGA CACCATGCTG ATCATGCAGC GCCTGGAGGC CGAGCGCAGA 
GCGCGGCAGA CTGCTGTGCC AGCAATCTCT GTTCTAGATT TGTTGCAGGA CGAGGAGCGG 
CGGCGGCAGC AGCAGCTGGA GGAGATGCGG CAGCGGGAGG CGGAGGAGCG TGCCCGCCAC 
GAGGAGGAGC GGCGGCGCCA GGAGGAGGAG CGGCTGAAGC GCGAGGCCGA GGAAAAGAGG 
CGCCAGGAGG AGGGGTACTA CAGCCGCCTG GAGGCCGAGC GGCGCAGACA GCACGAGGAG 
GCCGAGCGCC GGCTGCTGGA GCCCGAGGAG CCGGGCCTGG GCCGCCCCCC GCTCCCGCGG 
GGCTACGAGC CCCCCGGCCC CGCGCCGCCG CCCCCGCCCC AGCGCGCCGC CTCCTACCTG 
CAGGCCCAGG TCCTGTCCCC CGACTCCCTG TACACCGCCA AGTTCGTGGC TTACGACGAC 
GATGACGAGG AGGAGGACGG CCCTGCAGGT CAGGATAAGT ACGCCCGCGC CAGGGGGGCC 
CGCGGGGGCC CGCGCCCGGC CTCCGATGGC ATCTTTCTCG GCAGCTCCGC CCAGGCGCCC 
TGGGCCAGGG CCAACAGTCC TGCGCACAGG GCAGGCGCGC CCCCGGCCCC GCCGCCCAAG 
CCCGGCCCCG CCCGAGGCCG AGGGCCCGCC TCGTACATGG GATCTACGGG GACGCTGATG 
GGCGCCCACG ATGGCTCTCG GGACCCAAGA GAGACGCTGT CCAAAAGCCA GGACGCGGAC 
GTGCCGGGCG GGCCTGAGAG CCTGACCTTC CGGGAGCGCC AGCGCCTCTT CTCCCAGGGC 
CACGAGGTCT CCAGTAAGGT GAAAGCCTCC CGCCGGCTGA CGGAGCTGGA GAACGAGCTG 
AACACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984285.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839739.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.

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CloneID	OSe30611
Clone ID Related Accession (Same CDS sequence)	XM_013984285.1
Accession Version	XM_013984285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGATGCGGCT GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG TTGCAGGACG AGGAGCGGCG GCGGCAGCAG 
CAGCTGGAGG AGATGCGGCA GCGGGAGGCG GAGGAGCGTG CCCGCCACGA GGAGGAGCGG 
CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC GAGGCCGAGG AAAAGAGGCG CCAGGAGGAG 
GGGTACTACA GCCGCCTGGA GGCCGAGCGG CGCAGACAGC ACGAGGAGGC CGAGCGCCGG 
CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC CGCCCCCCGC TCCCGCGGGG CTACGAGCCC 
CCCGGCCCCG CGCCGCCGCC CCCGCCCCAG CGCGCCGCCT CCTACCTGCA GGCCCAGGTC 
CTGTCCCCCG ACTCCCTGTA CACCGCCAAG TTCGTGGCTT ACGACGACGA TGACGAGGAG 
GAGGACGGCC CTGCAGGTCA GGATAAGTAC GCCCGCGCCA GGGGGGCCCG CGGGGGCCCG 
CGCCCGGCCT CCGATGGCAT CTTTCTCGGC AGCTCCGCCC AGGCGCCCTG GGCCAGGGCC 
AACAGTCCTG CGCACAGGGC AGGCGCGCCC CCGGCCCCGC CGCCCAAGCC CGGCCCCGCC 
CGAGGCCGAG GGCCCGCCTC GTACATGGGA TCTACGGGGA CGCTGATGGG CGCCCACGAT 
GGCTCTCGGG ACCCAAGAGA GACGCTGTCC AAAAGCCAGG ACGCGGACGT GCCGGGCGGG 
CCTGAGAGCC TGACCTTCCG GGAGCGCCAG CGCCTCTTCT CCCAGGGCCA CGAGGTCTCC 
AGTAAGGTGA AAGCCTCCCG CCGGCTGACG GAGCTGGAGA ACGAGCTGAA CACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839739.1
CDS	22..5481
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984285.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984285.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGATGCGGCT GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG TTGCAGGACG AGGAGCGGCG GCGGCAGCAG 
CAGCTGGAGG AGATGCGGCA GCGGGAGGCG GAGGAGCGTG CCCGCCACGA GGAGGAGCGG 
CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC GAGGCCGAGG AAAAGAGGCG CCAGGAGGAG 
GGGTACTACA GCCGCCTGGA GGCCGAGCGG CGCAGACAGC ACGAGGAGGC CGAGCGCCGG 
CTGCTGGAGC CCGAGGAGCC GGGCCTGGGC CGCCCCCCGC TCCCGCGGGG CTACGAGCCC 
CCCGGCCCCG CGCCGCCGCC CCCGCCCCAG CGCGCCGCCT CCTACCTGCA GGCCCAGGTC 
CTGTCCCCCG ACTCCCTGTA CACCGCCAAG TTCGTGGCTT ACGACGACGA TGACGAGGAG 
GAGGACGGCC CTGCAGGTCA GGATAAGTAC GCCCGCGCCA GGGGGGCCCG CGGGGGCCCG 
CGCCCGGCCT CCGATGGCAT CTTTCTCGGC AGCTCCGCCC AGGCGCCCTG GGCCAGGGCC 
AACAGTCCTG CGCACAGGGC AGGCGCGCCC CCGGCCCCGC CGCCCAAGCC CGGCCCCGCC 
CGAGGCCGAG GGCCCGCCTC GTACATGGGA TCTACGGGGA CGCTGATGGG CGCCCACGAT 
GGCTCTCGGG ACCCAAGAGA GACGCTGTCC AAAAGCCAGG ACGCGGACGT GCCGGGCGGG 
CCTGAGAGCC TGACCTTCCG GGAGCGCCAG CGCCTCTTCT CCCAGGGCCA CGAGGTCTCC 
AGTAAGGTGA AAGCCTCCCG CCGGCTGACG GAGCTGGAGA ACGAGCTGAA CACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984289.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839743.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

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CloneID	OSe30612
Clone ID Related Accession (Same CDS sequence)	XM_013984289.1
Accession Version	XM_013984289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGACGGCT CCGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTCCAGTT AAGCGTCACC GAGGTCGGGA CGGAGAAGTT CGATGACAGC 
TCGATCCAGT TGTTTGGTTC CGGAATCCAG CCCCACCACT GCGACCTCAC CAACATGGAC 
GGGGTGGTCA CTGTCACGCC GAGAAGCGTG GATGCTGAGA CCTACGTGGA AGGACAGCGC 
ATCTCGGAGA CGACCATGCT GCAGAGCGGG GTGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCGCTCGCCA AGAGGCCGGG GGACGGAGGC 
CTGGCCGTCA AGGGTCCCAG ACACAAACCG GGAATCGTTC AGGAGACAGC TTTCGATTTA 
GGAGGAGACG TTCACGGGGG GACGGCCGTA GCGAGCAGTA AGGGCTCCGC CCGACCGGAC 
GGCGACAGGG CCCCGTCTGC CTCCAGCAGC GCCGAGCGCG GGATGGTGAA GCCGATGGTG 
CGAATGGAGC CGCCGCAGGA GCCCCGCAGG CAGGACACCA GACCGCAGGA CGCCGCTGGG 
CCTGAGCTGA CGCTGCCCGC GAGCATCGAA TTCAGGGAAA GTGCTGAAGA CTCGTTCTTA 
TCCGCCATCA TCAATTACAC GAATAGCTCG ACGGTGCATT TTAAGTTGTC GCCTACGTAC 
GTGCTGTACA TGGCGTGTCG GCACGTGCTG TCCAGCCAGT ACCGCCCGGA CGTCAGCCCC 
GCCGAGCGCA CGCACAAGGT CATCGCCATC GTCAACAAGA TGGTGAGCAT GATGGACGGC 
GTCATCCAGG AAGTAGACCA GGTGGCTGAG AAACAGAAGA ACATCGCGGG GGCGCTTGCG 
TTCTGGATGG CCAACGCGTC GGAACTCCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGAATCACAC TGGACGCCCA GGATGTTTTG GCTCACCTGG TTCAGATGGC CTTTAAATAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAC AACTACATGC CGGCCTTTCT GGACGACCCC 
GAGGAGAACA GTCTGCAAAG ACCAAAAATA GAGGACGTCC TCCACACGCT CACGGGCGCC 
ATGTCGCTGC TGCGGCGCTG CCGGGTCAAC GCCGCCCTCA CCATCCAGCT CTTCTCCCAG 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACAGGCTGC TGACCGACCC CGAGTCGGGC 
CTGTGCTCCC ACTACTGGGG CGCCATCGTC CGCCAGCAGC TGGGCCACGT GGAGGCCTGG 
GCGGAGAAGC AGGGCCTGGA GCTGGCTGCC GACTGCCACC TCAGCAGGGT CGTGCAGGCG 
ACCACCCTGC TCACGATGGA CAAGTACGCC CCGGAGGATG CGGCTGGCAT CAACAGCACC 
TGCTTCAAGC TGAACTCGCT GCAGCTGCAG GCCTTGCTGC ACAACTACCA CTGTGCGCCC 
GACGAGCCCC TGGTCCCGCC GGATCTGATA GAAAGCGTGG TGGCCGTGGC TGAAAACACG 
GCGGACGAGC TGGCGCGCAG CGACGGCAGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCCT TCCTGCTGCC GGAGGACGGC TACTCGTGTG ACGTCGTCAG AAACATCCCC 
AACGGCTTGC AGGAGTTTTT GGATCCTTTG TGCCAGAGAG GGTTTTGCAG GCTGACGCCC 
CAGCCGCGCT CTCCGGGTTC CTGGACGATA CATTTTGAAG GTGCGGAGTA CGAGAGCCGC 
CTCGCACGGG AGAGCACGGA GCTGGCTCAG CCTCTGAGGA AAGAACCCGA AGTCATCACT 
GTGACCCTGA AGAAGCAGAA CGGGATGGGC CTCAGCATCG TCGCAGCGAA GGGCGCAGGT 
CAAGACAAAC TCGGGATCTA CGTCAAGTCG GTGGTGAGAG GAGGAGCTGC AGACGTGGAC 
GGGCGGCTGG CGGCGGGGGA CCAGCTCCTC AGTGTGGACG GACGAAGCCT GGTGGGCCTC 
TCTCAGGAAA GGGCAGCAGA GCTGATGACA AGGACAAGTT CCGTGGTGAC CCTGGAGGTA 
GCGAAGCAGG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC CTCGCCCATG 
ATGCAGAGAA TTTCAGACCG GCGTGGATCA GGTAAACCCC GACCAAAGAG CGAAGGTTTT 
GAGCTCTATA ATAACTCAGC TCAGAACGGG TCCCCCGAGA GCCCCCCGCT GCCCTGGGCC 
GAGTACAGCG AGCCAAAGAA GCTGCCTGGC GACGAGAGGC TGACGAAGAA CCGCGCCGAC 
CACCGCTCCA GCCCCAACGT TGCAAATCAG CCTCCTAGTC CTGGTGGGAA AGGTGCACAT 
GCCGCTGGAA CAGCAGCCAA GATCACATCC GTCTCCACTG GCAACCTCTG CACCGAGGAG 
CAGACGCCCC CGCCGGGGAC GGAGGCCTAC CCCATCCCCA CGCAGGCCTA CCCCCGAGAG 
TACTTCACCT TCCCGGCCTC CAGGTCCCAG GACCGCGTGG TCCCCCCCCA GAGCCAGTGG 
CCAAGCTACG AGGAGAAGCC GCACGCGGAC AGCAGCCACT CCAGCCTCGC GGTTCAGCGG 
GTGACCCGCT CCCAGGAGGA GCTGCGGGAG GACGCGGGGG CCTACCCACT GCACAGGATG 
GAGGCGGTGA TGGACCGCAA GTCTGACAGC GACATGTGGG TCGCCCAGAG CTCCTCGATG 
GGCTCCAGCG CGTCCAGCCA GGAGCACCTG GACCCCTCTT CCAAGGCGGG CACCCCCGCG 
TCCACCCTCA CCAAGAGCGG CCCGGGGCGC TGGAAGACCC CTGCAGTTCT GCAGCCCGCG 
CCCGGCCCCC AGCCGCTCCG GACCGACCTG CCCACGCACT ACGCCGGCGA CGTGGACGGC 
GTCCCCGTGG ACCTGCCCCT CCCGCCGCCT CCGGCTGCCG CGGGCCAGGG ACTCGCGGGG 
GGCCAAGGGG CCGCCGAGCG GAAGAAGCGG GAGGAGCAGC AGCGCTGGTA CGAGAAGGAG 
AAGGCGCGCC TGGAGGAGGA GCGTGAGCGC AAGCGGCGCG AGCAGGAGCG CAAGCTGGGC 
CAGATGCGCT CGCAGGCGCT GCTCCCCGCC CCGCTCTCCC CCGGCGCCAC CAAGCCCGCC 
GCCCCGGGGC CCCAGGACAC CGTCATCCGG GAGCTGCAGC CTCAGCAGCA GCCGCGCACC 
ATCGAGCGCA GGGACCTGCA GTACATCACC GTCAGCCGGG AGGAGCTCTC GGCCGGCGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGCGAC GCGCGGGAGA AGCTGGAGAA GCAGCAGCAG 
ATGCACGTGG TGGACCTGCT GAGCCGGGAG ATTCACGAGC TGCAGAGCAA GGCCGAGCGC 
TCGGCGGAGG AGAGCGGCCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGCGG 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGACGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTGGAGGCC GAGCGCAGAG CGCGGCAGAC TGCTGTGCCA 
GCAATCTCTG TTCTAGATTT GTTGCAGGAC GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG 
GAGATGCGGC AGCGGGAGGC GGAGGAGCGT GCCCGCCACG AGGAGGAGCG GCGGCGCCAG 
GAGGAGGAGC GGCTGAAGCG CGAGGCCGAG GAAAAGAGGC GCCAGGAGGA GGGGTACTAC 
AGCCGCCTGG AGGCCGAGCG GCGCAGACAG CACGAGGAGG CCGAGCGCCG GCTGCTGGAG 
CCCGAGGAGC CGGGCCTGGG CCGCCCCCCG CTCCCGCGGG GCTACGAGCC CCCCGGCCCC 
GCGCCGCCGC CCCCGCCCCA GCGCGCCGCC TCCTACCTGC AGGCCCAGGT CCTGTCCCCC 
GACTCCCTGT ACACCGCCAA GTTCGTGGCT TACGACGACG ATGACGAGGA GGAGGACGGC 
CCTGCAGGTC AGGATAAGTA CGCCCGCGCC AGGGGGGCCC GCGGGGGCCC GCGCCCGGCC 
TCCGATGGCA TCTTTCTCGG CAGCTCCGCC CAGGCGCCCT GGGCCAGGGC CAACAGTCCT 
GCGCACAGGG CAGGCGCGCC CCCGGCCCCG CCGCCCAAGC CCGGCCCCGC CCGAGGCCGA 
GGGCCCGCCT CGTACATGGG ATCTACGGGG ACGCTGATGG GCGCCCACGA TGGCTCTCGG 
GACCCAAGAG AGACGCTGTC CAAAAGCCAG GACGCGGACG TGCCGGGCGG GCCTGAGAGC 
CTGACCTTCC GGGAGCGCCA GCGCCTCTTC TCCCAGGGCC ACGAGGTCTC CAGTAAGGTG 
AAAGCCTCCC GCCGGCTGAC GGAGCTGGAG AACGAGCTGA ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839743.1
CDS	22..5472
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984289.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984289.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGACGGCT CCGACTCCAG AGATAAGCCA 
AAGCTTTACC GCCTCCAGTT AAGCGTCACC GAGGTCGGGA CGGAGAAGTT CGATGACAGC 
TCGATCCAGT TGTTTGGTTC CGGAATCCAG CCCCACCACT GCGACCTCAC CAACATGGAC 
GGGGTGGTCA CTGTCACGCC GAGAAGCGTG GATGCTGAGA CCTACGTGGA AGGACAGCGC 
ATCTCGGAGA CGACCATGCT GCAGAGCGGG GTGAAGGTGC AGTTCGGGGC GTCCCACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCGCTCGCCA AGAGGCCGGG GGACGGAGGC 
CTGGCCGTCA AGGGTCCCAG ACACAAACCG GGAATCGTTC AGGAGACAGC TTTCGATTTA 
GGAGGAGACG TTCACGGGGG GACGGCCGTA GCGAGCAGTA AGGGCTCCGC CCGACCGGAC 
GGCGACAGGG CCCCGTCTGC CTCCAGCAGC GCCGAGCGCG GGATGGTGAA GCCGATGGTG 
CGAATGGAGC CGCCGCAGGA GCCCCGCAGG CAGGACACCA GACCGCAGGA CGCCGCTGGG 
CCTGAGCTGA CGCTGCCCGC GAGCATCGAA TTCAGGGAAA GTGCTGAAGA CTCGTTCTTA 
TCCGCCATCA TCAATTACAC GAATAGCTCG ACGGTGCATT TTAAGTTGTC GCCTACGTAC 
GTGCTGTACA TGGCGTGTCG GCACGTGCTG TCCAGCCAGT ACCGCCCGGA CGTCAGCCCC 
GCCGAGCGCA CGCACAAGGT CATCGCCATC GTCAACAAGA TGGTGAGCAT GATGGACGGC 
GTCATCCAGG AAGTAGACCA GGTGGCTGAG AAACAGAAGA ACATCGCGGG GGCGCTTGCG 
TTCTGGATGG CCAACGCGTC GGAACTCCTC AACTTCATCA AGCAGGACCG AGACCTCAGT 
CGAATCACAC TGGACGCCCA GGATGTTTTG GCTCACCTGG TTCAGATGGC CTTTAAATAC 
CTGGTTCACT GTCTTCAGTC AGAGCTGAAC AACTACATGC CGGCCTTTCT GGACGACCCC 
GAGGAGAACA GTCTGCAAAG ACCAAAAATA GAGGACGTCC TCCACACGCT CACGGGCGCC 
ATGTCGCTGC TGCGGCGCTG CCGGGTCAAC GCCGCCCTCA CCATCCAGCT CTTCTCCCAG 
CTCTTCCACT TCATCAACAT GTGGCTGTTC AACAGGCTGC TGACCGACCC CGAGTCGGGC 
CTGTGCTCCC ACTACTGGGG CGCCATCGTC CGCCAGCAGC TGGGCCACGT GGAGGCCTGG 
GCGGAGAAGC AGGGCCTGGA GCTGGCTGCC GACTGCCACC TCAGCAGGGT CGTGCAGGCG 
ACCACCCTGC TCACGATGGA CAAGTACGCC CCGGAGGATG CGGCTGGCAT CAACAGCACC 
TGCTTCAAGC TGAACTCGCT GCAGCTGCAG GCCTTGCTGC ACAACTACCA CTGTGCGCCC 
GACGAGCCCC TGGTCCCGCC GGATCTGATA GAAAGCGTGG TGGCCGTGGC TGAAAACACG 
GCGGACGAGC TGGCGCGCAG CGACGGCAGG GAGGTGCGGC TGGAGGAGGA CCCTGACCTG 
CAGCTGCCCT TCCTGCTGCC GGAGGACGGC TACTCGTGTG ACGTCGTCAG AAACATCCCC 
AACGGCTTGC AGGAGTTTTT GGATCCTTTG TGCCAGAGAG GGTTTTGCAG GCTGACGCCC 
CAGCCGCGCT CTCCGGGTTC CTGGACGATA CATTTTGAAG GTGCGGAGTA CGAGAGCCGC 
CTCGCACGGG AGAGCACGGA GCTGGCTCAG CCTCTGAGGA AAGAACCCGA AGTCATCACT 
GTGACCCTGA AGAAGCAGAA CGGGATGGGC CTCAGCATCG TCGCAGCGAA GGGCGCAGGT 
CAAGACAAAC TCGGGATCTA CGTCAAGTCG GTGGTGAGAG GAGGAGCTGC AGACGTGGAC 
GGGCGGCTGG CGGCGGGGGA CCAGCTCCTC AGTGTGGACG GACGAAGCCT GGTGGGCCTC 
TCTCAGGAAA GGGCAGCAGA GCTGATGACA AGGACAAGTT CCGTGGTGAC CCTGGAGGTA 
GCGAAGCAGG GAGCCATTTA CCACGGCCTC GCCACCCTGC TCAACCAGCC CTCGCCCATG 
ATGCAGAGAA TTTCAGACCG GCGTGGATCA GGTAAACCCC GACCAAAGAG CGAAGGTTTT 
GAGCTCTATA ATAACTCAGC TCAGAACGGG TCCCCCGAGA GCCCCCCGCT GCCCTGGGCC 
GAGTACAGCG AGCCAAAGAA GCTGCCTGGC GACGAGAGGC TGACGAAGAA CCGCGCCGAC 
CACCGCTCCA GCCCCAACGT TGCAAATCAG CCTCCTAGTC CTGGTGGGAA AGGTGCACAT 
GCCGCTGGAA CAGCAGCCAA GATCACATCC GTCTCCACTG GCAACCTCTG CACCGAGGAG 
CAGACGCCCC CGCCGGGGAC GGAGGCCTAC CCCATCCCCA CGCAGGCCTA CCCCCGAGAG 
TACTTCACCT TCCCGGCCTC CAGGTCCCAG GACCGCGTGG TCCCCCCCCA GAGCCAGTGG 
CCAAGCTACG AGGAGAAGCC GCACGCGGAC AGCAGCCACT CCAGCCTCGC GGTTCAGCGG 
GTGACCCGCT CCCAGGAGGA GCTGCGGGAG GACGCGGGGG CCTACCCACT GCACAGGATG 
GAGGCGGTGA TGGACCGCAA GTCTGACAGC GACATGTGGG TCGCCCAGAG CTCCTCGATG 
GGCTCCAGCG CGTCCAGCCA GGAGCACCTG GACCCCTCTT CCAAGGCGGG CACCCCCGCG 
TCCACCCTCA CCAAGAGCGG CCCGGGGCGC TGGAAGACCC CTGCAGTTCT GCAGCCCGCG 
CCCGGCCCCC AGCCGCTCCG GACCGACCTG CCCACGCACT ACGCCGGCGA CGTGGACGGC 
GTCCCCGTGG ACCTGCCCCT CCCGCCGCCT CCGGCTGCCG CGGGCCAGGG ACTCGCGGGG 
GGCCAAGGGG CCGCCGAGCG GAAGAAGCGG GAGGAGCAGC AGCGCTGGTA CGAGAAGGAG 
AAGGCGCGCC TGGAGGAGGA GCGTGAGCGC AAGCGGCGCG AGCAGGAGCG CAAGCTGGGC 
CAGATGCGCT CGCAGGCGCT GCTCCCCGCC CCGCTCTCCC CCGGCGCCAC CAAGCCCGCC 
GCCCCGGGGC CCCAGGACAC CGTCATCCGG GAGCTGCAGC CTCAGCAGCA GCCGCGCACC 
ATCGAGCGCA GGGACCTGCA GTACATCACC GTCAGCCGGG AGGAGCTCTC GGCCGGCGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGCGAC GCGCGGGAGA AGCTGGAGAA GCAGCAGCAG 
ATGCACGTGG TGGACCTGCT GAGCCGGGAG ATTCACGAGC TGCAGAGCAA GGCCGAGCGC 
TCGGCGGAGG AGAGCGGCCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGCGG 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGACGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTGGAGGCC GAGCGCAGAG CGCGGCAGAC TGCTGTGCCA 
GCAATCTCTG TTCTAGATTT GTTGCAGGAC GAGGAGCGGC GGCGGCAGCA GCAGCTGGAG 
GAGATGCGGC AGCGGGAGGC GGAGGAGCGT GCCCGCCACG AGGAGGAGCG GCGGCGCCAG 
GAGGAGGAGC GGCTGAAGCG CGAGGCCGAG GAAAAGAGGC GCCAGGAGGA GGGGTACTAC 
AGCCGCCTGG AGGCCGAGCG GCGCAGACAG CACGAGGAGG CCGAGCGCCG GCTGCTGGAG 
CCCGAGGAGC CGGGCCTGGG CCGCCCCCCG CTCCCGCGGG GCTACGAGCC CCCCGGCCCC 
GCGCCGCCGC CCCCGCCCCA GCGCGCCGCC TCCTACCTGC AGGCCCAGGT CCTGTCCCCC 
GACTCCCTGT ACACCGCCAA GTTCGTGGCT TACGACGACG ATGACGAGGA GGAGGACGGC 
CCTGCAGGTC AGGATAAGTA CGCCCGCGCC AGGGGGGCCC GCGGGGGCCC GCGCCCGGCC 
TCCGATGGCA TCTTTCTCGG CAGCTCCGCC CAGGCGCCCT GGGCCAGGGC CAACAGTCCT 
GCGCACAGGG CAGGCGCGCC CCCGGCCCCG CCGCCCAAGC CCGGCCCCGC CCGAGGCCGA 
GGGCCCGCCT CGTACATGGG ATCTACGGGG ACGCTGATGG GCGCCCACGA TGGCTCTCGG 
GACCCAAGAG AGACGCTGTC CAAAAGCCAG GACGCGGACG TGCCGGGCGG GCCTGAGAGC 
CTGACCTTCC GGGAGCGCCA GCGCCTCTTC TCCCAGGGCC ACGAGGTCTC CAGTAAGGTG 
AAAGCCTCCC GCCGGCTGAC GGAGCTGGAG AACGAGCTGA ACACCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984292.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839746.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.

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CloneID	OSe30613
Clone ID Related Accession (Same CDS sequence)	XM_013984292.1
Accession Version	XM_013984292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGGC TCAAAGTAAC 
GGACCTGAAA AGCAGGAAAA AGAAGGCGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG 
AAAGAAAAGA AGGAAAAAAA GAAGAGAGAG AAAGAGGCGA TGAGACAAGC ATCTGATAAA 
GATGATAGAC CCTTCCACGG GGAGGACACT GAGAATTCTC GACTGGCTGC CGAGGTTTAC 
AAAGACATGC CCGAAACTAG CTTTACCCGA ACAATCTCCA ACCCCGAGGT GGTCATGAAG 
CGAAGGCGGC AGCAAAAACT GGAAAAGAGG ATGCAGGAGT TTCGGAGCTC AGACGGGCGC 
CCTGATTCCG GTGGAACGTT GAGAATTTAT GCGGATAGTT TAAAGCCAAA TATTCCCTAC 
AAGACAATCC TGCTGTCCAC CACGGACCCC GCCGACTTTG CCGTGGCGGA GGCTTTGGAG 
AAGTACGGTC TGGAGAAGGA GAACCCGAAG GATTACTGCA TCGCCCGGGT TATGCTTCCT 
CCTGGAGCCC AGCATTCGGA TGAGAAAGGT GCTAAAGAAA TTATCCTGGA CGATGACGAG 
TGTCCTTTAC AGATCTTCAG GGAATGGCCT GGTGACAAAG GGATGCTGGT CTTCCAGCTG 
AAGAGGAGGC CCCCAGAGCA CATCCCCAAG AAGAGCAAGA AGCACGCGGA GGGGAAGCCG 
CCCAAGGGGA AGGAGCGGCC CGACGGCTCT GGCTATGGCT CTGCGCTCCC CCCGGAGAAG 
CTGCCCTACC TAGTGGAGCT GAGCCCAGAC GGCTCCGACT CCAGAGATAA GCCAAAGCTT 
TACCGCCTCC AGTTAAGCGT CACCGAGGTC GGGACGGAGA AGTTCGATGA CAGCTCGATC 
CAGTTGTTTG GTTCCGGAAT CCAGCCCCAC CACTGCGACC TCACCAACAT GGACGGGGTG 
GTCACTGTCA CGCCGAGAAG CGTGGATGCT GAGACCTACG TGGAAGGACA GCGCATCTCG 
GAGACGACCA TGCTGCAGAG CGGGGTGAAG GTGCAGTTCG GGGCGTCCCA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCGCTC GCCAAGAGGC CGGGGGACGG AGGCCTGGCC 
GTCAAGGGTC CCAGACACAA ACCGGGAATC GTTCAGGAGA CAGCTTTCGA TTTAGGAGGA 
GACGTTCACG GGGGGACGGC CGTAGCGAGC AGTAAGGGCT CCGCCCGACC GGACGGCGAC 
AGGGCCCCGT CTGCCTCCAG CAGCGCCGAG CGCGGGATGG TGAAGCCGAT GGTGCGAATG 
GAGCCGCCGC AGGAGCCCCG CAGGCAGGAC ACCAGACCGC AGGACGCCGC TGGGCCTGAG 
CTGACGCTGC CCGCGAGCAT CGAATTCAGG GAAAGTGCTG AAGACTCGTT CTTATCCGCC 
ATCATCAATT ACACGAATAG CTCGACGGTG CATTTTAAGT TGTCGCCTAC GTACGTGCTG 
TACATGGCGT GTCGGCACGT GCTGTCCAGC CAGTACCGCC CGGACGTCAG CCCCGCCGAG 
CGCACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA CGGCGTCATC 
CAGAAACAGA AGAACATCGC GGGGGCGCTT GCGTTCTGGA TGGCCAACGC GTCGGAACTC 
CTCAACTTCA TCAAGCAGGA CCGAGACCTC AGTCGAATCA CACTGGACGC CCAGGATGTT 
TTGGCTCACC TGGTTCAGAT GGCCTTTAAA TACCTGGTTC ACTGTCTTCA GTCAGAGCTG 
AACAACTACA TGCCGGCCTT TCTGGACGAC CCCGAGGAGA ACAGTCTGCA AAGACCAAAA 
ATAGAGGACG TCCTCCACAC GCTCACGGGC GCCATGTCGC TGCTGCGGCG CTGCCGGGTC 
AACGCCGCCC TCACCATCCA GCTCTTCTCC CAGCTCTTCC ACTTCATCAA CATGTGGCTG 
TTCAACAGGC TGCTGACCGA CCCCGAGTCG GGCCTGTGCT CCCACTACTG GGGCGCCATC 
GTCCGCCAGC AGCTGGGCCA CGTGGAGGCC TGGGCGGAGA AGCAGGGCCT GGAGCTGGCT 
GCCGACTGCC ACCTCAGCAG GGTCGTGCAG GCGACCACCC TGCTCACGAT GGACAAGTAC 
GCCCCGGAGG ATGCGGCTGG CATCAACAGC ACCTGCTTCA AGCTGAACTC GCTGCAGCTG 
CAGGCCTTGC TGCACAACTA CCACTGTGCG CCCGACGAGC CCCTGGTCCC GCCGGATCTG 
ATAGAAAGCG TGGTGGCCGT GGCTGAAAAC ACGGCGGACG AGCTGGCGCG CAGCGACGGC 
AGGGAGGTGC GGCTGGAGGA GGACCCTGAC CTGCAGCTGC CCTTCCTGCT GCCGGAGGAC 
GGCTACTCGT GTGACGTCGT CAGAAACATC CCCAACGGCT TGCAGGAGTT TTTGGATCCT 
TTGTGCCAGA GAGGGTTTTG CAGGCTGACG CCCCAGCCGC GCTCTCCGGG TTCCTGGACG 
ATACATTTTG AAGGTGCGGA GTACGAGAGC CGCCTCGCAC GGGAGAGCAC GGAGCTGGCT 
CAGCCTCTGA GGAAAGAACC CGAAGTCATC ACTGTGACCC TGAAGAAGCA GAACGGGATG 
GGCCTCAGCA TCGTCGCAGC GAAGGGCGCA GGTCAAGACA AACTCGGGAT CTACGTCAAG 
TCGGTGGTGA GAGGAGGAGC TGCAGACGTG GACGGGCGGC TGGCGGCGGG GGACCAGCTC 
CTCAGTGTGG ACGGACGAAG CCTGGTGGGC CTCTCTCAGG AAAGGGCAGC AGAGCTGATG 
ACAAGGACAA GTTCCGTGGT GACCCTGGAG GTAGCGAAGC AGGGAGCCAT TTACCACGGC 
CTCGCCACCC TGCTCAACCA GCCCTCGCCC ATGATGCAGA GAATTTCAGA CCGGCGTGGA 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTTGAGCTCT ATAATAACTC AGCTCAGAAC 
GGGTCCCCCG AGAGCCCCCC GCTGCCCTGG GCCGAGTACA GCGAGCCAAA GAAGCTGCCT 
GGCGACGAGA GGCTGACGAA GAACCGCGCC GACCACCGCT CCAGCCCCAA CGTTGCAAAT 
CAGCCTCCTA GTCCTGGTGG GAAAGGTGCA CATGCCGCTG GAACAGCAGC CAAGATCACA 
TCCGTCTCCA CTGGCAACCT CTGCACCGAG GAGCAGACGC CCCCGCCGGG GACGGAGGCC 
TACCCCATCC CCACGCAGGC CTACCCCCGA GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGTCCCCCC CCAGAGCCAG TGGCCAAGCT ACGAGGAGAA GCCGCACGCG 
GACAGCAGCC ACTCCAGCCT CGCGGTTCAG CGGGTGACCC GCTCCCAGGA GGAGCTGCGG 
GAGGACGCGG GGGCCTACCC ACTGCACAGG ATGGAGGCGG TGATGGACCG CAAGTCTGAC 
AGCGACATGT GGGTCGCCCA GAGCTCCTCG ATGGGCTCCA GCGCGTCCAG CCAGGAGCAC 
CTGGACCCCT CTTCCAAGGC GGGCACCCCC GCGTCCACCC TCACCAAGAG CGGCCCGGGG 
CGCTGGAAGA CCCCTGCAGT TCTGCAGCCC GCGCCCGGCC CCCAGCCGCT CCGGACCGAC 
CTGCCCACGC ACTACGCCGG CGACGTGGAC GGCGTCCCCG TGGACCTGCC CCTCCCGCCG 
CCTCCGGCTG CCGCGGGCCA GGGACTCGCG GGGGGCCAAG GGGCCGCCGA GCGGAAGAAG 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCGC GCCTGGAGGA GGAGCGTGAG 
CGCAAGCGGC GCGAGCAGGA GCGCAAGCTG GGCCAGATGC GCTCGCAGGC GCTGCTCCCC 
GCCCCGCTCT CCCCCGGCGC CACCAAGCCC GCCGCCCCGG GGCCCCAGGA CACCGTCATC 
CGGGAGCTGC AGCCTCAGCA GCAGCCGCGC ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACCGTCAGCC GGGAGGAGCT CTCGGCCGGC GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GACGCGCGGG AGAAGCTGGA GAAGCAGCAG CAGATGCACG TGGTGGACCT GCTGAGCCGG 
GAGATTCACG AGCTGCAGAG CAAGGCCGAG CGCTCGGCGG AGGAGAGCGG CCGCCTGCGC 
AAGCTCATGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGACG AGGAGGAGGA CGACGACGTG GACACCATGC TGATCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTGTG CCAGCAATCT CTGTTCTAGA TTTGTTGCAG 
GACGAGGAGC GGCGGCGGCA GCAGCAGCTG GAGGAGATGC GGCAGCGGGA GGCGGAGGAG 
CGTGCCCGCC ACGAGGAGGA GCGGCGGCGC CAGGAGGAGG AGCGGCTGAA GCGCGAGGCC 
GAGGAAAAGA GGCGCCAGGA GGAGGGGTAC TACAGCCGCC TGGAGGCCGA GCGGCGCAGA 
CAGCACGAGG AGGCCGAGCG CCGGCTGCTG GAGCCCGAGG AGCCGGGCCT GGGCCGCCCC 
CCGCTCCCGC GGGGCTACGA GCCCCCCGGC CCCGCGCCGC CGCCCCCGCC CCAGCGCGCC 
GCCTCCTACC TGCAGGCCCA GGTCCTGTCC CCCGACTCCC TGTACACCGC CAAGTTCGTG 
GCTTACGACG ACGATGACGA GGAGGAGGAC GGCCCTGCAG GTCAGGATAA GTACGCCCGC 
GCCAGGGGGG CCCGCGGGGG CCCGCGCCCG GCCTCCGATG GCATCTTTCT CGGCAGCTCC 
GCCCAGGCGC CCTGGGCCAG GGCCAACAGT CCTGCGCACA GGGCAGGCGC GCCCCCGGCC 
CCGCCGCCCA AGCCCGGCCC CGCCCGAGGC CGAGGGCCCG CCTCGTACAT GGGATCTACG 
GGGACGCTGA TGGGCGCCCA CGATGGCTCT CGGGACCCAA GAGAGACGCT GTCCAAAAGC 
CAGGACGCGG ACGTGCCGGG CGGGCCTGAG AGCCTGACCT TCCGGGAGCG CCAGCGCCTC 
TTCTCCCAGG GCCACGAGGT CTCCAGTAAG GTGAAAGCCT CCCGCCGGCT GACGGAGCTG 
GAGAACGAGC TGAACACCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839746.1
CDS	22..5445
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984292.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984292.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGGC TCAAAGTAAC 
GGACCTGAAA AGCAGGAAAA AGAAGGCGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG 
AAAGAAAAGA AGGAAAAAAA GAAGAGAGAG AAAGAGGCGA TGAGACAAGC ATCTGATAAA 
GATGATAGAC CCTTCCACGG GGAGGACACT GAGAATTCTC GACTGGCTGC CGAGGTTTAC 
AAAGACATGC CCGAAACTAG CTTTACCCGA ACAATCTCCA ACCCCGAGGT GGTCATGAAG 
CGAAGGCGGC AGCAAAAACT GGAAAAGAGG ATGCAGGAGT TTCGGAGCTC AGACGGGCGC 
CCTGATTCCG GTGGAACGTT GAGAATTTAT GCGGATAGTT TAAAGCCAAA TATTCCCTAC 
AAGACAATCC TGCTGTCCAC CACGGACCCC GCCGACTTTG CCGTGGCGGA GGCTTTGGAG 
AAGTACGGTC TGGAGAAGGA GAACCCGAAG GATTACTGCA TCGCCCGGGT TATGCTTCCT 
CCTGGAGCCC AGCATTCGGA TGAGAAAGGT GCTAAAGAAA TTATCCTGGA CGATGACGAG 
TGTCCTTTAC AGATCTTCAG GGAATGGCCT GGTGACAAAG GGATGCTGGT CTTCCAGCTG 
AAGAGGAGGC CCCCAGAGCA CATCCCCAAG AAGAGCAAGA AGCACGCGGA GGGGAAGCCG 
CCCAAGGGGA AGGAGCGGCC CGACGGCTCT GGCTATGGCT CTGCGCTCCC CCCGGAGAAG 
CTGCCCTACC TAGTGGAGCT GAGCCCAGAC GGCTCCGACT CCAGAGATAA GCCAAAGCTT 
TACCGCCTCC AGTTAAGCGT CACCGAGGTC GGGACGGAGA AGTTCGATGA CAGCTCGATC 
CAGTTGTTTG GTTCCGGAAT CCAGCCCCAC CACTGCGACC TCACCAACAT GGACGGGGTG 
GTCACTGTCA CGCCGAGAAG CGTGGATGCT GAGACCTACG TGGAAGGACA GCGCATCTCG 
GAGACGACCA TGCTGCAGAG CGGGGTGAAG GTGCAGTTCG GGGCGTCCCA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCGCTC GCCAAGAGGC CGGGGGACGG AGGCCTGGCC 
GTCAAGGGTC CCAGACACAA ACCGGGAATC GTTCAGGAGA CAGCTTTCGA TTTAGGAGGA 
GACGTTCACG GGGGGACGGC CGTAGCGAGC AGTAAGGGCT CCGCCCGACC GGACGGCGAC 
AGGGCCCCGT CTGCCTCCAG CAGCGCCGAG CGCGGGATGG TGAAGCCGAT GGTGCGAATG 
GAGCCGCCGC AGGAGCCCCG CAGGCAGGAC ACCAGACCGC AGGACGCCGC TGGGCCTGAG 
CTGACGCTGC CCGCGAGCAT CGAATTCAGG GAAAGTGCTG AAGACTCGTT CTTATCCGCC 
ATCATCAATT ACACGAATAG CTCGACGGTG CATTTTAAGT TGTCGCCTAC GTACGTGCTG 
TACATGGCGT GTCGGCACGT GCTGTCCAGC CAGTACCGCC CGGACGTCAG CCCCGCCGAG 
CGCACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA CGGCGTCATC 
CAGAAACAGA AGAACATCGC GGGGGCGCTT GCGTTCTGGA TGGCCAACGC GTCGGAACTC 
CTCAACTTCA TCAAGCAGGA CCGAGACCTC AGTCGAATCA CACTGGACGC CCAGGATGTT 
TTGGCTCACC TGGTTCAGAT GGCCTTTAAA TACCTGGTTC ACTGTCTTCA GTCAGAGCTG 
AACAACTACA TGCCGGCCTT TCTGGACGAC CCCGAGGAGA ACAGTCTGCA AAGACCAAAA 
ATAGAGGACG TCCTCCACAC GCTCACGGGC GCCATGTCGC TGCTGCGGCG CTGCCGGGTC 
AACGCCGCCC TCACCATCCA GCTCTTCTCC CAGCTCTTCC ACTTCATCAA CATGTGGCTG 
TTCAACAGGC TGCTGACCGA CCCCGAGTCG GGCCTGTGCT CCCACTACTG GGGCGCCATC 
GTCCGCCAGC AGCTGGGCCA CGTGGAGGCC TGGGCGGAGA AGCAGGGCCT GGAGCTGGCT 
GCCGACTGCC ACCTCAGCAG GGTCGTGCAG GCGACCACCC TGCTCACGAT GGACAAGTAC 
GCCCCGGAGG ATGCGGCTGG CATCAACAGC ACCTGCTTCA AGCTGAACTC GCTGCAGCTG 
CAGGCCTTGC TGCACAACTA CCACTGTGCG CCCGACGAGC CCCTGGTCCC GCCGGATCTG 
ATAGAAAGCG TGGTGGCCGT GGCTGAAAAC ACGGCGGACG AGCTGGCGCG CAGCGACGGC 
AGGGAGGTGC GGCTGGAGGA GGACCCTGAC CTGCAGCTGC CCTTCCTGCT GCCGGAGGAC 
GGCTACTCGT GTGACGTCGT CAGAAACATC CCCAACGGCT TGCAGGAGTT TTTGGATCCT 
TTGTGCCAGA GAGGGTTTTG CAGGCTGACG CCCCAGCCGC GCTCTCCGGG TTCCTGGACG 
ATACATTTTG AAGGTGCGGA GTACGAGAGC CGCCTCGCAC GGGAGAGCAC GGAGCTGGCT 
CAGCCTCTGA GGAAAGAACC CGAAGTCATC ACTGTGACCC TGAAGAAGCA GAACGGGATG 
GGCCTCAGCA TCGTCGCAGC GAAGGGCGCA GGTCAAGACA AACTCGGGAT CTACGTCAAG 
TCGGTGGTGA GAGGAGGAGC TGCAGACGTG GACGGGCGGC TGGCGGCGGG GGACCAGCTC 
CTCAGTGTGG ACGGACGAAG CCTGGTGGGC CTCTCTCAGG AAAGGGCAGC AGAGCTGATG 
ACAAGGACAA GTTCCGTGGT GACCCTGGAG GTAGCGAAGC AGGGAGCCAT TTACCACGGC 
CTCGCCACCC TGCTCAACCA GCCCTCGCCC ATGATGCAGA GAATTTCAGA CCGGCGTGGA 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTTGAGCTCT ATAATAACTC AGCTCAGAAC 
GGGTCCCCCG AGAGCCCCCC GCTGCCCTGG GCCGAGTACA GCGAGCCAAA GAAGCTGCCT 
GGCGACGAGA GGCTGACGAA GAACCGCGCC GACCACCGCT CCAGCCCCAA CGTTGCAAAT 
CAGCCTCCTA GTCCTGGTGG GAAAGGTGCA CATGCCGCTG GAACAGCAGC CAAGATCACA 
TCCGTCTCCA CTGGCAACCT CTGCACCGAG GAGCAGACGC CCCCGCCGGG GACGGAGGCC 
TACCCCATCC CCACGCAGGC CTACCCCCGA GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGTCCCCCC CCAGAGCCAG TGGCCAAGCT ACGAGGAGAA GCCGCACGCG 
GACAGCAGCC ACTCCAGCCT CGCGGTTCAG CGGGTGACCC GCTCCCAGGA GGAGCTGCGG 
GAGGACGCGG GGGCCTACCC ACTGCACAGG ATGGAGGCGG TGATGGACCG CAAGTCTGAC 
AGCGACATGT GGGTCGCCCA GAGCTCCTCG ATGGGCTCCA GCGCGTCCAG CCAGGAGCAC 
CTGGACCCCT CTTCCAAGGC GGGCACCCCC GCGTCCACCC TCACCAAGAG CGGCCCGGGG 
CGCTGGAAGA CCCCTGCAGT TCTGCAGCCC GCGCCCGGCC CCCAGCCGCT CCGGACCGAC 
CTGCCCACGC ACTACGCCGG CGACGTGGAC GGCGTCCCCG TGGACCTGCC CCTCCCGCCG 
CCTCCGGCTG CCGCGGGCCA GGGACTCGCG GGGGGCCAAG GGGCCGCCGA GCGGAAGAAG 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCGC GCCTGGAGGA GGAGCGTGAG 
CGCAAGCGGC GCGAGCAGGA GCGCAAGCTG GGCCAGATGC GCTCGCAGGC GCTGCTCCCC 
GCCCCGCTCT CCCCCGGCGC CACCAAGCCC GCCGCCCCGG GGCCCCAGGA CACCGTCATC 
CGGGAGCTGC AGCCTCAGCA GCAGCCGCGC ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACCGTCAGCC GGGAGGAGCT CTCGGCCGGC GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GACGCGCGGG AGAAGCTGGA GAAGCAGCAG CAGATGCACG TGGTGGACCT GCTGAGCCGG 
GAGATTCACG AGCTGCAGAG CAAGGCCGAG CGCTCGGCGG AGGAGAGCGG CCGCCTGCGC 
AAGCTCATGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGACG AGGAGGAGGA CGACGACGTG GACACCATGC TGATCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGCA GACTGCTGTG CCAGCAATCT CTGTTCTAGA TTTGTTGCAG 
GACGAGGAGC GGCGGCGGCA GCAGCAGCTG GAGGAGATGC GGCAGCGGGA GGCGGAGGAG 
CGTGCCCGCC ACGAGGAGGA GCGGCGGCGC CAGGAGGAGG AGCGGCTGAA GCGCGAGGCC 
GAGGAAAAGA GGCGCCAGGA GGAGGGGTAC TACAGCCGCC TGGAGGCCGA GCGGCGCAGA 
CAGCACGAGG AGGCCGAGCG CCGGCTGCTG GAGCCCGAGG AGCCGGGCCT GGGCCGCCCC 
CCGCTCCCGC GGGGCTACGA GCCCCCCGGC CCCGCGCCGC CGCCCCCGCC CCAGCGCGCC 
GCCTCCTACC TGCAGGCCCA GGTCCTGTCC CCCGACTCCC TGTACACCGC CAAGTTCGTG 
GCTTACGACG ACGATGACGA GGAGGAGGAC GGCCCTGCAG GTCAGGATAA GTACGCCCGC 
GCCAGGGGGG CCCGCGGGGG CCCGCGCCCG GCCTCCGATG GCATCTTTCT CGGCAGCTCC 
GCCCAGGCGC CCTGGGCCAG GGCCAACAGT CCTGCGCACA GGGCAGGCGC GCCCCCGGCC 
CCGCCGCCCA AGCCCGGCCC CGCCCGAGGC CGAGGGCCCG CCTCGTACAT GGGATCTACG 
GGGACGCTGA TGGGCGCCCA CGATGGCTCT CGGGACCCAA GAGAGACGCT GTCCAAAAGC 
CAGGACGCGG ACGTGCCGGG CGGGCCTGAG AGCCTGACCT TCCGGGAGCG CCAGCGCCTC 
TTCTCCCAGG GCCACGAGGT CTCCAGTAAG GTGAAAGCCT CCCGCCGGCT GACGGAGCTG 
GAGAACGAGC TGAACACCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984296.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839750.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA.

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CloneID	OSe30614
Clone ID Related Accession (Same CDS sequence)	XM_013984296.1
Accession Version	XM_013984296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGATGCGGCT GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG CAGACTGCTG TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGCAGCGG 
GAGGCGGAGG AGCGTGCCCG CCACGAGGAG GAGCGGCGGC GCCAGGAGGA GGAGCGGCTG 
AAGCGCGAGG CCGAGGAAAA GAGGCGCCAG GAGGAGGGGT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCA GACAGCACGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CCCCGCTCCC GCGGGGCTAC GAGCCCCCCG GCCCCGCGCC GCCGCCCCCG 
CCCCAGCGCG CCGCCTCCTA CCTGCAGGCC CAGGTCCTGT CCCCCGACTC CCTGTACACC 
GCCAAGTTCG TGGCTTACGA CGACGATGAC GAGGAGGAGG ACGGCCCTGC AGGGCCCGCC 
TCGTACATGG GATCTACGGG GACGCTGATG GGCGCCCACG ATGGCTCTCG GGACCCAAGA 
GAGACGCTGT CCAAAAGCCA GGACGCGGAC GTGCCGGGCG GGCCTGAGAG CCTGACCTTC 
CGGGAGCGCC AGCGCCTCTT CTCCCAGGGC CACGAGGTCT CCAGTAAGGT GAAAGCCTCC 
CGCCGGCTGA CGGAGCTGGA GAACGAGCTG AACACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839750.1
CDS	22..5343
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQEVDQVAEKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARQTAVPAISVLDLLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984296.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984296.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGGAAGTA 
GACCAGGTGG CTGAGAAACA GAAGAACATC GCGGGGGCGC TTGCGTTCTG GATGGCCAAC 
GCGTCGGAAC TCCTCAACTT CATCAAGCAG GACCGAGACC TCAGTCGAAT CACACTGGAC 
GCCCAGGATG TTTTGGCTCA CCTGGTTCAG ATGGCCTTTA AATACCTGGT TCACTGTCTT 
CAGTCAGAGC TGAACAACTA CATGCCGGCC TTTCTGGACG ACCCCGAGGA GAACAGTCTG 
CAAAGACCAA AAATAGAGGA CGTCCTCCAC ACGCTCACGG GCGCCATGTC GCTGCTGCGG 
CGCTGCCGGG TCAACGCCGC CCTCACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCATC 
AACATGTGGC TGTTCAACAG GCTGCTGACC GACCCCGAGT CGGGCCTGTG CTCCCACTAC 
TGGGGCGCCA TCGTCCGCCA GCAGCTGGGC CACGTGGAGG CCTGGGCGGA GAAGCAGGGC 
CTGGAGCTGG CTGCCGACTG CCACCTCAGC AGGGTCGTGC AGGCGACCAC CCTGCTCACG 
ATGGACAAGT ACGCCCCGGA GGATGCGGCT GGCATCAACA GCACCTGCTT CAAGCTGAAC 
TCGCTGCAGC TGCAGGCCTT GCTGCACAAC TACCACTGTG CGCCCGACGA GCCCCTGGTC 
CCGCCGGATC TGATAGAAAG CGTGGTGGCC GTGGCTGAAA ACACGGCGGA CGAGCTGGCG 
CGCAGCGACG GCAGGGAGGT GCGGCTGGAG GAGGACCCTG ACCTGCAGCT GCCCTTCCTG 
CTGCCGGAGG ACGGCTACTC GTGTGACGTC GTCAGAAACA TCCCCAACGG CTTGCAGGAG 
TTTTTGGATC CTTTGTGCCA GAGAGGGTTT TGCAGGCTGA CGCCCCAGCC GCGCTCTCCG 
GGTTCCTGGA CGATACATTT TGAAGGTGCG GAGTACGAGA GCCGCCTCGC ACGGGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCCGAAGTCA TCACTGTGAC CCTGAAGAAG 
CAGAACGGGA TGGGCCTCAG CATCGTCGCA GCGAAGGGCG CAGGTCAAGA CAAACTCGGG 
ATCTACGTCA AGTCGGTGGT GAGAGGAGGA GCTGCAGACG TGGACGGGCG GCTGGCGGCG 
GGGGACCAGC TCCTCAGTGT GGACGGACGA AGCCTGGTGG GCCTCTCTCA GGAAAGGGCA 
GCAGAGCTGA TGACAAGGAC AAGTTCCGTG GTGACCCTGG AGGTAGCGAA GCAGGGAGCC 
ATTTACCACG GCCTCGCCAC CCTGCTCAAC CAGCCCTCGC CCATGATGCA GAGAATTTCA 
GACCGGCGTG GATCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTTGAGCT CTATAATAAC 
TCAGCTCAGA ACGGGTCCCC CGAGAGCCCC CCGCTGCCCT GGGCCGAGTA CAGCGAGCCA 
AAGAAGCTGC CTGGCGACGA GAGGCTGACG AAGAACCGCG CCGACCACCG CTCCAGCCCC 
AACGTTGCAA ATCAGCCTCC TAGTCCTGGT GGGAAAGGTG CACATGCCGC TGGAACAGCA 
GCCAAGATCA CATCCGTCTC CACTGGCAAC CTCTGCACCG AGGAGCAGAC GCCCCCGCCG 
GGGACGGAGG CCTACCCCAT CCCCACGCAG GCCTACCCCC GAGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGTCCCC CCCCAGAGCC AGTGGCCAAG CTACGAGGAG 
AAGCCGCACG CGGACAGCAG CCACTCCAGC CTCGCGGTTC AGCGGGTGAC CCGCTCCCAG 
GAGGAGCTGC GGGAGGACGC GGGGGCCTAC CCACTGCACA GGATGGAGGC GGTGATGGAC 
CGCAAGTCTG ACAGCGACAT GTGGGTCGCC CAGAGCTCCT CGATGGGCTC CAGCGCGTCC 
AGCCAGGAGC ACCTGGACCC CTCTTCCAAG GCGGGCACCC CCGCGTCCAC CCTCACCAAG 
AGCGGCCCGG GGCGCTGGAA GACCCCTGCA GTTCTGCAGC CCGCGCCCGG CCCCCAGCCG 
CTCCGGACCG ACCTGCCCAC GCACTACGCC GGCGACGTGG ACGGCGTCCC CGTGGACCTG 
CCCCTCCCGC CGCCTCCGGC TGCCGCGGGC CAGGGACTCG CGGGGGGCCA AGGGGCCGCC 
GAGCGGAAGA AGCGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC GCGCCTGGAG 
GAGGAGCGTG AGCGCAAGCG GCGCGAGCAG GAGCGCAAGC TGGGCCAGAT GCGCTCGCAG 
GCGCTGCTCC CCGCCCCGCT CTCCCCCGGC GCCACCAAGC CCGCCGCCCC GGGGCCCCAG 
GACACCGTCA TCCGGGAGCT GCAGCCTCAG CAGCAGCCGC GCACCATCGA GCGCAGGGAC 
CTGCAGTACA TCACCGTCAG CCGGGAGGAG CTCTCGGCCG GCGACAGCCT GTCCCCCGAC 
CCCTGGAAGC GCGACGCGCG GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CGTGGTGGAC 
CTGCTGAGCC GGGAGATTCA CGAGCTGCAG AGCAAGGCCG AGCGCTCGGC GGAGGAGAGC 
GGCCGCCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGCGGCTGCA GGAGTCCAAG 
CAGAAGGACG AGGACGACGA CGAGGAGGAG GACGACGACG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCCGAGCG CAGAGCGCGG CAGACTGCTG TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGCAGCGG 
GAGGCGGAGG AGCGTGCCCG CCACGAGGAG GAGCGGCGGC GCCAGGAGGA GGAGCGGCTG 
AAGCGCGAGG CCGAGGAAAA GAGGCGCCAG GAGGAGGGGT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCA GACAGCACGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CCCCGCTCCC GCGGGGCTAC GAGCCCCCCG GCCCCGCGCC GCCGCCCCCG 
CCCCAGCGCG CCGCCTCCTA CCTGCAGGCC CAGGTCCTGT CCCCCGACTC CCTGTACACC 
GCCAAGTTCG TGGCTTACGA CGACGATGAC GAGGAGGAGG ACGGCCCTGC AGGGCCCGCC 
TCGTACATGG GATCTACGGG GACGCTGATG GGCGCCCACG ATGGCTCTCG GGACCCAAGA 
GAGACGCTGT CCAAAAGCCA GGACGCGGAC GTGCCGGGCG GGCCTGAGAG CCTGACCTTC 
CGGGAGCGCC AGCGCCTCTT CTCCCAGGGC CACGAGGTCT CCAGTAAGGT GAAAGCCTCC 
CGCCGGCTGA CGGAGCTGGA GAACGAGCTG AACACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984300.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839754.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA.

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CloneID	OSe30615
Clone ID Related Accession (Same CDS sequence)	XM_013984300.1
Accession Version	XM_013984300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GAAACAGAAG 
AACATCGCGG GGGCGCTTGC GTTCTGGATG GCCAACGCGT CGGAACTCCT CAACTTCATC 
AAGCAGGACC GAGACCTCAG TCGAATCACA CTGGACGCCC AGGATGTTTT GGCTCACCTG 
GTTCAGATGG CCTTTAAATA CCTGGTTCAC TGTCTTCAGT CAGAGCTGAA CAACTACATG 
CCGGCCTTTC TGGACGACCC CGAGGAGAAC AGTCTGCAAA GACCAAAAAT AGAGGACGTC 
CTCCACACGC TCACGGGCGC CATGTCGCTG CTGCGGCGCT GCCGGGTCAA CGCCGCCCTC 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCATCAACA TGTGGCTGTT CAACAGGCTG 
CTGACCGACC CCGAGTCGGG CCTGTGCTCC CACTACTGGG GCGCCATCGT CCGCCAGCAG 
CTGGGCCACG TGGAGGCCTG GGCGGAGAAG CAGGGCCTGG AGCTGGCTGC CGACTGCCAC 
CTCAGCAGGG TCGTGCAGGC GACCACCCTG CTCACGATGG ACAAGTACGC CCCGGAGGAT 
GCGGCTGGCA TCAACAGCAC CTGCTTCAAG CTGAACTCGC TGCAGCTGCA GGCCTTGCTG 
CACAACTACC ACTGTGCGCC CGACGAGCCC CTGGTCCCGC CGGATCTGAT AGAAAGCGTG 
GTGGCCGTGG CTGAAAACAC GGCGGACGAG CTGGCGCGCA GCGACGGCAG GGAGGTGCGG 
CTGGAGGAGG ACCCTGACCT GCAGCTGCCC TTCCTGCTGC CGGAGGACGG CTACTCGTGT 
GACGTCGTCA GAAACATCCC CAACGGCTTG CAGGAGTTTT TGGATCCTTT GTGCCAGAGA 
GGGTTTTGCA GGCTGACGCC CCAGCCGCGC TCTCCGGGTT CCTGGACGAT ACATTTTGAA 
GGTGCGGAGT ACGAGAGCCG CCTCGCACGG GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCCG AAGTCATCAC TGTGACCCTG AAGAAGCAGA ACGGGATGGG CCTCAGCATC 
GTCGCAGCGA AGGGCGCAGG TCAAGACAAA CTCGGGATCT ACGTCAAGTC GGTGGTGAGA 
GGAGGAGCTG CAGACGTGGA CGGGCGGCTG GCGGCGGGGG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGCC TGGTGGGCCT CTCTCAGGAA AGGGCAGCAG AGCTGATGAC AAGGACAAGT 
TCCGTGGTGA CCCTGGAGGT AGCGAAGCAG GGAGCCATTT ACCACGGCCT CGCCACCCTG 
CTCAACCAGC CCTCGCCCAT GATGCAGAGA ATTTCAGACC GGCGTGGATC AGGTAAACCC 
CGACCAAAGA GCGAAGGTTT TGAGCTCTAT AATAACTCAG CTCAGAACGG GTCCCCCGAG 
AGCCCCCCGC TGCCCTGGGC CGAGTACAGC GAGCCAAAGA AGCTGCCTGG CGACGAGAGG 
CTGACGAAGA ACCGCGCCGA CCACCGCTCC AGCCCCAACG TTGCAAATCA GCCTCCTAGT 
CCTGGTGGGA AAGGTGCACA TGCCGCTGGA ACAGCAGCCA AGATCACATC CGTCTCCACT 
GGCAACCTCT GCACCGAGGA GCAGACGCCC CCGCCGGGGA CGGAGGCCTA CCCCATCCCC 
ACGCAGGCCT ACCCCCGAGA GTACTTCACC TTCCCGGCCT CCAGGTCCCA GGACCGCGTG 
GTCCCCCCCC AGAGCCAGTG GCCAAGCTAC GAGGAGAAGC CGCACGCGGA CAGCAGCCAC 
TCCAGCCTCG CGGTTCAGCG GGTGACCCGC TCCCAGGAGG AGCTGCGGGA GGACGCGGGG 
GCCTACCCAC TGCACAGGAT GGAGGCGGTG ATGGACCGCA AGTCTGACAG CGACATGTGG 
GTCGCCCAGA GCTCCTCGAT GGGCTCCAGC GCGTCCAGCC AGGAGCACCT GGACCCCTCT 
TCCAAGGCGG GCACCCCCGC GTCCACCCTC ACCAAGAGCG GCCCGGGGCG CTGGAAGACC 
CCTGCAGTTC TGCAGCCCGC GCCCGGCCCC CAGCCGCTCC GGACCGACCT GCCCACGCAC 
TACGCCGGCG ACGTGGACGG CGTCCCCGTG GACCTGCCCC TCCCGCCGCC TCCGGCTGCC 
GCGGGCCAGG GACTCGCGGG GGGCCAAGGG GCCGCCGAGC GGAAGAAGCG GGAGGAGCAG 
CAGCGCTGGT ACGAGAAGGA GAAGGCGCGC CTGGAGGAGG AGCGTGAGCG CAAGCGGCGC 
GAGCAGGAGC GCAAGCTGGG CCAGATGCGC TCGCAGGCGC TGCTCCCCGC CCCGCTCTCC 
CCCGGCGCCA CCAAGCCCGC CGCCCCGGGG CCCCAGGACA CCGTCATCCG GGAGCTGCAG 
CCTCAGCAGC AGCCGCGCAC CATCGAGCGC AGGGACCTGC AGTACATCAC CGTCAGCCGG 
GAGGAGCTCT CGGCCGGCGA CAGCCTGTCC CCCGACCCCT GGAAGCGCGA CGCGCGGGAG 
AAGCTGGAGA AGCAGCAGCA GATGCACGTG GTGGACCTGC TGAGCCGGGA GATTCACGAG 
CTGCAGAGCA AGGCCGAGCG CTCGGCGGAG GAGAGCGGCC GCCTGCGCAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGACGAG 
GAGGAGGACG ACGACGTGGA CACCATGCTG ATCATGCAGC GCCTGGAGGC CGAGCGCAGA 
GCGCGGTTGC AGGACGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGCAGCGG 
GAGGCGGAGG AGCGTGCCCG CCACGAGGAG GAGCGGCGGC GCCAGGAGGA GGAGCGGCTG 
AAGCGCGAGG CCGAGGAAAA GAGGCGCCAG GAGGAGGGGT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCA GACAGCACGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CCCCGCTCCC GCGGGGCTAC GAGCCCCCCG GCCCCGCGCC GCCGCCCCCG 
CCCCAGCGCG CCGCCTCCTA CCTGCAGGCC CAGGTCCTGT CCCCCGACTC CCTGTACACC 
GCCAAGTTCG TGGCTTACGA CGACGATGAC GAGGAGGAGG ACGGCCCTGC AGGGCCCGCC 
TCGTACATGG GATCTACGGG GACGCTGATG GGCGCCCACG ATGGCTCTCG GGACCCAAGA 
GAGACGCTGT CCAAAAGCCA GGACGCGGAC GTGCCGGGCG GGCCTGAGAG CCTGACCTTC 
CGGGAGCGCC AGCGCCTCTT CTCCCAGGGC CACGAGGTCT CCAGTAAGGT GAAAGCCTCC 
CGCCGGCTGA CGGAGCTGGA GAACGAGCTG AACACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839754.1
CDS	22..5283
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984300.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984300.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GAAACAGAAG 
AACATCGCGG GGGCGCTTGC GTTCTGGATG GCCAACGCGT CGGAACTCCT CAACTTCATC 
AAGCAGGACC GAGACCTCAG TCGAATCACA CTGGACGCCC AGGATGTTTT GGCTCACCTG 
GTTCAGATGG CCTTTAAATA CCTGGTTCAC TGTCTTCAGT CAGAGCTGAA CAACTACATG 
CCGGCCTTTC TGGACGACCC CGAGGAGAAC AGTCTGCAAA GACCAAAAAT AGAGGACGTC 
CTCCACACGC TCACGGGCGC CATGTCGCTG CTGCGGCGCT GCCGGGTCAA CGCCGCCCTC 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCATCAACA TGTGGCTGTT CAACAGGCTG 
CTGACCGACC CCGAGTCGGG CCTGTGCTCC CACTACTGGG GCGCCATCGT CCGCCAGCAG 
CTGGGCCACG TGGAGGCCTG GGCGGAGAAG CAGGGCCTGG AGCTGGCTGC CGACTGCCAC 
CTCAGCAGGG TCGTGCAGGC GACCACCCTG CTCACGATGG ACAAGTACGC CCCGGAGGAT 
GCGGCTGGCA TCAACAGCAC CTGCTTCAAG CTGAACTCGC TGCAGCTGCA GGCCTTGCTG 
CACAACTACC ACTGTGCGCC CGACGAGCCC CTGGTCCCGC CGGATCTGAT AGAAAGCGTG 
GTGGCCGTGG CTGAAAACAC GGCGGACGAG CTGGCGCGCA GCGACGGCAG GGAGGTGCGG 
CTGGAGGAGG ACCCTGACCT GCAGCTGCCC TTCCTGCTGC CGGAGGACGG CTACTCGTGT 
GACGTCGTCA GAAACATCCC CAACGGCTTG CAGGAGTTTT TGGATCCTTT GTGCCAGAGA 
GGGTTTTGCA GGCTGACGCC CCAGCCGCGC TCTCCGGGTT CCTGGACGAT ACATTTTGAA 
GGTGCGGAGT ACGAGAGCCG CCTCGCACGG GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCCG AAGTCATCAC TGTGACCCTG AAGAAGCAGA ACGGGATGGG CCTCAGCATC 
GTCGCAGCGA AGGGCGCAGG TCAAGACAAA CTCGGGATCT ACGTCAAGTC GGTGGTGAGA 
GGAGGAGCTG CAGACGTGGA CGGGCGGCTG GCGGCGGGGG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGCC TGGTGGGCCT CTCTCAGGAA AGGGCAGCAG AGCTGATGAC AAGGACAAGT 
TCCGTGGTGA CCCTGGAGGT AGCGAAGCAG GGAGCCATTT ACCACGGCCT CGCCACCCTG 
CTCAACCAGC CCTCGCCCAT GATGCAGAGA ATTTCAGACC GGCGTGGATC AGGTAAACCC 
CGACCAAAGA GCGAAGGTTT TGAGCTCTAT AATAACTCAG CTCAGAACGG GTCCCCCGAG 
AGCCCCCCGC TGCCCTGGGC CGAGTACAGC GAGCCAAAGA AGCTGCCTGG CGACGAGAGG 
CTGACGAAGA ACCGCGCCGA CCACCGCTCC AGCCCCAACG TTGCAAATCA GCCTCCTAGT 
CCTGGTGGGA AAGGTGCACA TGCCGCTGGA ACAGCAGCCA AGATCACATC CGTCTCCACT 
GGCAACCTCT GCACCGAGGA GCAGACGCCC CCGCCGGGGA CGGAGGCCTA CCCCATCCCC 
ACGCAGGCCT ACCCCCGAGA GTACTTCACC TTCCCGGCCT CCAGGTCCCA GGACCGCGTG 
GTCCCCCCCC AGAGCCAGTG GCCAAGCTAC GAGGAGAAGC CGCACGCGGA CAGCAGCCAC 
TCCAGCCTCG CGGTTCAGCG GGTGACCCGC TCCCAGGAGG AGCTGCGGGA GGACGCGGGG 
GCCTACCCAC TGCACAGGAT GGAGGCGGTG ATGGACCGCA AGTCTGACAG CGACATGTGG 
GTCGCCCAGA GCTCCTCGAT GGGCTCCAGC GCGTCCAGCC AGGAGCACCT GGACCCCTCT 
TCCAAGGCGG GCACCCCCGC GTCCACCCTC ACCAAGAGCG GCCCGGGGCG CTGGAAGACC 
CCTGCAGTTC TGCAGCCCGC GCCCGGCCCC CAGCCGCTCC GGACCGACCT GCCCACGCAC 
TACGCCGGCG ACGTGGACGG CGTCCCCGTG GACCTGCCCC TCCCGCCGCC TCCGGCTGCC 
GCGGGCCAGG GACTCGCGGG GGGCCAAGGG GCCGCCGAGC GGAAGAAGCG GGAGGAGCAG 
CAGCGCTGGT ACGAGAAGGA GAAGGCGCGC CTGGAGGAGG AGCGTGAGCG CAAGCGGCGC 
GAGCAGGAGC GCAAGCTGGG CCAGATGCGC TCGCAGGCGC TGCTCCCCGC CCCGCTCTCC 
CCCGGCGCCA CCAAGCCCGC CGCCCCGGGG CCCCAGGACA CCGTCATCCG GGAGCTGCAG 
CCTCAGCAGC AGCCGCGCAC CATCGAGCGC AGGGACCTGC AGTACATCAC CGTCAGCCGG 
GAGGAGCTCT CGGCCGGCGA CAGCCTGTCC CCCGACCCCT GGAAGCGCGA CGCGCGGGAG 
AAGCTGGAGA AGCAGCAGCA GATGCACGTG GTGGACCTGC TGAGCCGGGA GATTCACGAG 
CTGCAGAGCA AGGCCGAGCG CTCGGCGGAG GAGAGCGGCC GCCTGCGCAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGACGAG 
GAGGAGGACG ACGACGTGGA CACCATGCTG ATCATGCAGC GCCTGGAGGC CGAGCGCAGA 
GCGCGGTTGC AGGACGAGGA GCGGCGGCGG CAGCAGCAGC TGGAGGAGAT GCGGCAGCGG 
GAGGCGGAGG AGCGTGCCCG CCACGAGGAG GAGCGGCGGC GCCAGGAGGA GGAGCGGCTG 
AAGCGCGAGG CCGAGGAAAA GAGGCGCCAG GAGGAGGGGT ACTACAGCCG CCTGGAGGCC 
GAGCGGCGCA GACAGCACGA GGAGGCCGAG CGCCGGCTGC TGGAGCCCGA GGAGCCGGGC 
CTGGGCCGCC CCCCGCTCCC GCGGGGCTAC GAGCCCCCCG GCCCCGCGCC GCCGCCCCCG 
CCCCAGCGCG CCGCCTCCTA CCTGCAGGCC CAGGTCCTGT CCCCCGACTC CCTGTACACC 
GCCAAGTTCG TGGCTTACGA CGACGATGAC GAGGAGGAGG ACGGCCCTGC AGGGCCCGCC 
TCGTACATGG GATCTACGGG GACGCTGATG GGCGCCCACG ATGGCTCTCG GGACCCAAGA 
GAGACGCTGT CCAAAAGCCA GGACGCGGAC GTGCCGGGCG GGCCTGAGAG CCTGACCTTC 
CGGGAGCGCC AGCGCCTCTT CTCCCAGGGC CACGAGGTCT CCAGTAAGGT GAAAGCCTCC 
CGCCGGCTGA CGGAGCTGGA GAACGAGCTG AACACCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984302.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839756.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.

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CloneID	OSe30616
Clone ID Related Accession (Same CDS sequence)	XM_013984302.1
Accession Version	XM_013984302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGAAACAG 
AAGAACATCG CGGGGGCGCT TGCGTTCTGG ATGGCCAACG CGTCGGAACT CCTCAACTTC 
ATCAAGCAGG ACCGAGACCT CAGTCGAATC ACACTGGACG CCCAGGATGT TTTGGCTCAC 
CTGGTTCAGA TGGCCTTTAA ATACCTGGTT CACTGTCTTC AGTCAGAGCT GAACAACTAC 
ATGCCGGCCT TTCTGGACGA CCCCGAGGAG AACAGTCTGC AAAGACCAAA AATAGAGGAC 
GTCCTCCACA CGCTCACGGG CGCCATGTCG CTGCTGCGGC GCTGCCGGGT CAACGCCGCC 
CTCACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCATCA ACATGTGGCT GTTCAACAGG 
CTGCTGACCG ACCCCGAGTC GGGCCTGTGC TCCCACTACT GGGGCGCCAT CGTCCGCCAG 
CAGCTGGGCC ACGTGGAGGC CTGGGCGGAG AAGCAGGGCC TGGAGCTGGC TGCCGACTGC 
CACCTCAGCA GGGTCGTGCA GGCGACCACC CTGCTCACGA TGGACAAGTA CGCCCCGGAG 
GATGCGGCTG GCATCAACAG CACCTGCTTC AAGCTGAACT CGCTGCAGCT GCAGGCCTTG 
CTGCACAACT ACCACTGTGC GCCCGACGAG CCCCTGGTCC CGCCGGATCT GATAGAAAGC 
GTGGTGGCCG TGGCTGAAAA CACGGCGGAC GAGCTGGCGC GCAGCGACGG CAGGGAGGTG 
CGGCTGGAGG AGGACCCTGA CCTGCAGCTG CCCTTCCTGC TGCCGGAGGA CGGCTACTCG 
TGTGACGTCG TCAGAAACAT CCCCAACGGC TTGCAGGAGT TTTTGGATCC TTTGTGCCAG 
AGAGGGTTTT GCAGGCTGAC GCCCCAGCCG CGCTCTCCGG GTTCCTGGAC GATACATTTT 
GAAGGTGCGG AGTACGAGAG CCGCCTCGCA CGGGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CCGAAGTCAT CACTGTGACC CTGAAGAAGC AGAACGGGAT GGGCCTCAGC 
ATCGTCGCAG CGAAGGGCGC AGGTCAAGAC AAACTCGGGA TCTACGTCAA GTCGGTGGTG 
AGAGGAGGAG CTGCAGACGT GGACGGGCGG CTGGCGGCGG GGGACCAGCT CCTCAGTGTG 
GACGGACGAA GCCTGGTGGG CCTCTCTCAG GAAAGGGCAG CAGAGCTGAT GACAAGGACA 
AGTTCCGTGG TGACCCTGGA GGTAGCGAAG CAGGGAGCCA TTTACCACGG CCTCGCCACC 
CTGCTCAACC AGCCCTCGCC CATGATGCAG AGAATTTCAG ACCGGCGTGG ATCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTTGAGCTC TATAATAACT CAGCTCAGAA CGGGTCCCCC 
GAGAGCCCCC CGCTGCCCTG GGCCGAGTAC AGCGAGCCAA AGAAGCTGCC TGGCGACGAG 
AGGCTGACGA AGAACCGCGC CGACCACCGC TCCAGCCCCA ACGTTGCAAA TCAGCCTCCT 
AGTCCTGGTG GGAAAGGTGC ACATGCCGCT GGAACAGCAG CCAAGATCAC ATCCGTCTCC 
ACTGGCAACC TCTGCACCGA GGAGCAGACG CCCCCGCCGG GGACGGAGGC CTACCCCATC 
CCCACGCAGG CCTACCCCCG AGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGTCCCCC CCCAGAGCCA GTGGCCAAGC TACGAGGAGA AGCCGCACGC GGACAGCAGC 
CACTCCAGCC TCGCGGTTCA GCGGGTGACC CGCTCCCAGG AGGAGCTGCG GGAGGACGCG 
GGGGCCTACC CACTGCACAG GATGGAGGCG GTGATGGACC GCAAGTCTGA CAGCGACATG 
TGGGTCGCCC AGAGCTCCTC GATGGGCTCC AGCGCGTCCA GCCAGGAGCA CCTGGACCCC 
TCTTCCAAGG CGGGCACCCC CGCGTCCACC CTCACCAAGA GCGGCCCGGG GCGCTGGAAG 
ACCCCTGCAG TTCTGCAGCC CGCGCCCGGC CCCCAGCCGC TCCGGACCGA CCTGCCCACG 
CACTACGCCG GCGACGTGGA CGGCGTCCCC GTGGACCTGC CCCTCCCGCC GCCTCCGGCT 
GCCGCGGGCC AGGGACTCGC GGGGGGCCAA GGGGCCGCCG AGCGGAAGAA GCGGGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAGAAGGCG CGCCTGGAGG AGGAGCGTGA GCGCAAGCGG 
CGCGAGCAGG AGCGCAAGCT GGGCCAGATG CGCTCGCAGG CGCTGCTCCC CGCCCCGCTC 
TCCCCCGGCG CCACCAAGCC CGCCGCCCCG GGGCCCCAGG ACACCGTCAT CCGGGAGCTG 
CAGCCTCAGC AGCAGCCGCG CACCATCGAG CGCAGGGACC TGCAGTACAT CACCGTCAGC 
CGGGAGGAGC TCTCGGCCGG CGACAGCCTG TCCCCCGACC CCTGGAAGCG CGACGCGCGG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC GTGGTGGACC TGCTGAGCCG GGAGATTCAC 
GAGCTGCAGA GCAAGGCCGA GCGCTCGGCG GAGGAGAGCG GCCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAC 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG GCCCGCCTCG 
TACATGGGAT CTACGGGGAC GCTGATGGGC GCCCACGATG GCTCTCGGGA CCCAAGAGAG 
ACGCTGTCCA AAAGCCAGGA CGCGGACGTG CCGGGCGGGC CTGAGAGCCT GACCTTCCGG 
GAGCGCCAGC GCCTCTTCTC CCAGGGCCAC GAGGTCTCCA GTAAGGTGAA AGCCTCCCGC 
CGGCTGACGG AGCTGGAGAA CGAGCTGAAC ACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839756.1
CDS	22..5280
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984302.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984302.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGACGA GAAGCCCCTA GTTGTGCAGC TGAATTGGAA TAAAGATGAC 
CGCGAGGGCA GGTTTGTCCT TAAGAACGAG AACGACGCCG CTCCTCCCAA GAAGGCTCAA 
AGTAACGGAC CTGAAAAGCA GGAAAAAGAA GGCGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAGAAAG AGGCGATGAG ACAAGCATCT 
GATAAAGATG ATAGACCCTT CCACGGGGAG GACACTGAGA ATTCTCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCCGA AACTAGCTTT ACCCGAACAA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGAA GGCGGCAGCA AAAACTGGAA AAGAGGATGC AGGAGTTTCG GAGCTCAGAC 
GGGCGCCCTG ATTCCGGTGG AACGTTGAGA ATTTATGCGG ATAGTTTAAA GCCAAATATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GACCCCGCCG ACTTTGCCGT GGCGGAGGCT 
TTGGAGAAGT ACGGTCTGGA GAAGGAGAAC CCGAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCGGATGAG AAAGGTGCTA AAGAAATTAT CCTGGACGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCTGGTG ACAAAGGGAT GCTGGTCTTC 
CAGCTGAAGA GGAGGCCCCC AGAGCACATC CCCAAGAAGA GCAAGAAGCA CGCGGAGGGG 
AAGCCGCCCA AGGGGAAGGA GCGGCCCGAC GGCTCTGGCT ATGGCTCTGC GCTCCCCCCG 
GAGAAGCTGC CCTACCTAGT GGAGCTGAGC CCAGGGAGAA GGGGTCACTT TGCCTACTAC 
AGCTATCACC CTCACGAAGA CGGCTCCGAC TCCAGAGATA AGCCAAAGCT TTACCGCCTC 
CAGTTAAGCG TCACCGAGGT CGGGACGGAG AAGTTCGATG ACAGCTCGAT CCAGTTGTTT 
GGTTCCGGAA TCCAGCCCCA CCACTGCGAC CTCACCAACA TGGACGGGGT GGTCACTGTC 
ACGCCGAGAA GCGTGGATGC TGAGACCTAC GTGGAAGGAC AGCGCATCTC GGAGACGACC 
ATGCTGCAGA GCGGGGTGAA GGTGCAGTTC GGGGCGTCCC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCGCT CGCCAAGAGG CCGGGGGACG GAGGCCTGGC CGTCAAGGGT 
CCCAGACACA AACCGGGAAT CGTTCAGGAG ACAGCTTTCG ATTTAGGAGG AGACGTTCAC 
GGGGGGACGG CCGTAGCGAG CAGTAAGGGC TCCGCCCGAC CGGACGGCGA CAGGGCCCCG 
TCTGCCTCCA GCAGCGCCGA GCGCGGGATG GTGAAGCCGA TGGTGCGAAT GGAGCCGCCG 
CAGGAGCCCC GCAGGCAGGA CACCAGACCG CAGGACGCCG CTGGGCCTGA GCTGACGCTG 
CCCGCGAGCA TCGAATTCAG GGAAAGTGCT GAAGACTCGT TCTTATCCGC CATCATCAAT 
TACACGAATA GCTCGACGGT GCATTTTAAG TTGTCGCCTA CGTACGTGCT GTACATGGCG 
TGTCGGCACG TGCTGTCCAG CCAGTACCGC CCGGACGTCA GCCCCGCCGA GCGCACGCAC 
AAGGTCATCG CCATCGTCAA CAAGATGGTG AGCATGATGG ACGGCGTCAT CCAGAAACAG 
AAGAACATCG CGGGGGCGCT TGCGTTCTGG ATGGCCAACG CGTCGGAACT CCTCAACTTC 
ATCAAGCAGG ACCGAGACCT CAGTCGAATC ACACTGGACG CCCAGGATGT TTTGGCTCAC 
CTGGTTCAGA TGGCCTTTAA ATACCTGGTT CACTGTCTTC AGTCAGAGCT GAACAACTAC 
ATGCCGGCCT TTCTGGACGA CCCCGAGGAG AACAGTCTGC AAAGACCAAA AATAGAGGAC 
GTCCTCCACA CGCTCACGGG CGCCATGTCG CTGCTGCGGC GCTGCCGGGT CAACGCCGCC 
CTCACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCATCA ACATGTGGCT GTTCAACAGG 
CTGCTGACCG ACCCCGAGTC GGGCCTGTGC TCCCACTACT GGGGCGCCAT CGTCCGCCAG 
CAGCTGGGCC ACGTGGAGGC CTGGGCGGAG AAGCAGGGCC TGGAGCTGGC TGCCGACTGC 
CACCTCAGCA GGGTCGTGCA GGCGACCACC CTGCTCACGA TGGACAAGTA CGCCCCGGAG 
GATGCGGCTG GCATCAACAG CACCTGCTTC AAGCTGAACT CGCTGCAGCT GCAGGCCTTG 
CTGCACAACT ACCACTGTGC GCCCGACGAG CCCCTGGTCC CGCCGGATCT GATAGAAAGC 
GTGGTGGCCG TGGCTGAAAA CACGGCGGAC GAGCTGGCGC GCAGCGACGG CAGGGAGGTG 
CGGCTGGAGG AGGACCCTGA CCTGCAGCTG CCCTTCCTGC TGCCGGAGGA CGGCTACTCG 
TGTGACGTCG TCAGAAACAT CCCCAACGGC TTGCAGGAGT TTTTGGATCC TTTGTGCCAG 
AGAGGGTTTT GCAGGCTGAC GCCCCAGCCG CGCTCTCCGG GTTCCTGGAC GATACATTTT 
GAAGGTGCGG AGTACGAGAG CCGCCTCGCA CGGGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CCGAAGTCAT CACTGTGACC CTGAAGAAGC AGAACGGGAT GGGCCTCAGC 
ATCGTCGCAG CGAAGGGCGC AGGTCAAGAC AAACTCGGGA TCTACGTCAA GTCGGTGGTG 
AGAGGAGGAG CTGCAGACGT GGACGGGCGG CTGGCGGCGG GGGACCAGCT CCTCAGTGTG 
GACGGACGAA GCCTGGTGGG CCTCTCTCAG GAAAGGGCAG CAGAGCTGAT GACAAGGACA 
AGTTCCGTGG TGACCCTGGA GGTAGCGAAG CAGGGAGCCA TTTACCACGG CCTCGCCACC 
CTGCTCAACC AGCCCTCGCC CATGATGCAG AGAATTTCAG ACCGGCGTGG ATCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTTGAGCTC TATAATAACT CAGCTCAGAA CGGGTCCCCC 
GAGAGCCCCC CGCTGCCCTG GGCCGAGTAC AGCGAGCCAA AGAAGCTGCC TGGCGACGAG 
AGGCTGACGA AGAACCGCGC CGACCACCGC TCCAGCCCCA ACGTTGCAAA TCAGCCTCCT 
AGTCCTGGTG GGAAAGGTGC ACATGCCGCT GGAACAGCAG CCAAGATCAC ATCCGTCTCC 
ACTGGCAACC TCTGCACCGA GGAGCAGACG CCCCCGCCGG GGACGGAGGC CTACCCCATC 
CCCACGCAGG CCTACCCCCG AGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGTCCCCC CCCAGAGCCA GTGGCCAAGC TACGAGGAGA AGCCGCACGC GGACAGCAGC 
CACTCCAGCC TCGCGGTTCA GCGGGTGACC CGCTCCCAGG AGGAGCTGCG GGAGGACGCG 
GGGGCCTACC CACTGCACAG GATGGAGGCG GTGATGGACC GCAAGTCTGA CAGCGACATG 
TGGGTCGCCC AGAGCTCCTC GATGGGCTCC AGCGCGTCCA GCCAGGAGCA CCTGGACCCC 
TCTTCCAAGG CGGGCACCCC CGCGTCCACC CTCACCAAGA GCGGCCCGGG GCGCTGGAAG 
ACCCCTGCAG TTCTGCAGCC CGCGCCCGGC CCCCAGCCGC TCCGGACCGA CCTGCCCACG 
CACTACGCCG GCGACGTGGA CGGCGTCCCC GTGGACCTGC CCCTCCCGCC GCCTCCGGCT 
GCCGCGGGCC AGGGACTCGC GGGGGGCCAA GGGGCCGCCG AGCGGAAGAA GCGGGAGGAG 
CAGCAGCGCT GGTACGAGAA GGAGAAGGCG CGCCTGGAGG AGGAGCGTGA GCGCAAGCGG 
CGCGAGCAGG AGCGCAAGCT GGGCCAGATG CGCTCGCAGG CGCTGCTCCC CGCCCCGCTC 
TCCCCCGGCG CCACCAAGCC CGCCGCCCCG GGGCCCCAGG ACACCGTCAT CCGGGAGCTG 
CAGCCTCAGC AGCAGCCGCG CACCATCGAG CGCAGGGACC TGCAGTACAT CACCGTCAGC 
CGGGAGGAGC TCTCGGCCGG CGACAGCCTG TCCCCCGACC CCTGGAAGCG CGACGCGCGG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC GTGGTGGACC TGCTGAGCCG GGAGATTCAC 
GAGCTGCAGA GCAAGGCCGA GCGCTCGGCG GAGGAGAGCG GCCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GCGGCTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAC 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCCGAGCGC 
AGAGCGCGGG ACGAGGAGCG GCGGCGGCAG CAGCAGCTGG AGGAGATGCG GCAGCGGGAG 
GCGGAGGAGC GTGCCCGCCA CGAGGAGGAG CGGCGGCGCC AGGAGGAGGA GCGGCTGAAG 
CGCGAGGCCG AGGAAAAGAG GCGCCAGGAG GAGGGGTACT ACAGCCGCCT GGAGGCCGAG 
CGGCGCAGAC AGCACGAGGA GGCCGAGCGC CGGCTGCTGG AGCCCGAGGA GCCGGGCCTG 
GGCCGCCCCC CGCTCCCGCG GGGCTACGAG CCCCCCGGCC CCGCGCCGCC GCCCCCGCCC 
CAGCGCGCCG CCTCCTACCT GCAGGCCCAG GTCCTGTCCC CCGACTCCCT GTACACCGCC 
AAGTTCGTGG CTTACGACGA CGATGACGAG GAGGAGGACG GCCCTGCAGG GCCCGCCTCG 
TACATGGGAT CTACGGGGAC GCTGATGGGC GCCCACGATG GCTCTCGGGA CCCAAGAGAG 
ACGCTGTCCA AAAGCCAGGA CGCGGACGTG CCGGGCGGGC CTGAGAGCCT GACCTTCCGG 
GAGCGCCAGC GCCTCTTCTC CCAGGGCCAC GAGGTCTCCA GTAAGGTGAA AGCCTCCCGC 
CGGCTGACGG AGCTGGAGAA CGAGCTGAAC ACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984304.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839758.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.

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CloneID	OSe30617
Clone ID Related Accession (Same CDS sequence)	XM_013984304.1
Accession Version	XM_013984304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGGC TCAAAGTAAC 
GGACCTGAAA AGCAGGAAAA AGAAGGCGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG 
AAAGAAAAGA AGGAAAAAAA GAAGAGAGAG AAAGAGGCGA TGAGACAAGC ATCTGATAAA 
GATGATAGAC CCTTCCACGG GGAGGACACT GAGAATTCTC GACTGGCTGC CGAGGTTTAC 
AAAGACATGC CCGAAACTAG CTTTACCCGA ACAATCTCCA ACCCCGAGGT GGTCATGAAG 
CGAAGGCGGC AGCAAAAACT GGAAAAGAGG ATGCAGGAGT TTCGGAGCTC AGACGGGCGC 
CCTGATTCCG GTGGAACGTT GAGAATTTAT GCGGATAGTT TAAAGCCAAA TATTCCCTAC 
AAGACAATCC TGCTGTCCAC CACGGACCCC GCCGACTTTG CCGTGGCGGA GGCTTTGGAG 
AAGTACGGTC TGGAGAAGGA GAACCCGAAG GATTACTGCA TCGCCCGGGT TATGCTTCCT 
CCTGGAGCCC AGCATTCGGA TGAGAAAGGT GCTAAAGAAA TTATCCTGGA CGATGACGAG 
TGTCCTTTAC AGATCTTCAG GGAATGGCCT GGTGACAAAG GGATGCTGGT CTTCCAGCTG 
AAGAGGAGGC CCCCAGAGCA CATCCCCAAG AAGAGCAAGA AGCACGCGGA GGGGAAGCCG 
CCCAAGGGGA AGGAGCGGCC CGACGGCTCT GGCTATGGCT CTGCGCTCCC CCCGGAGAAG 
CTGCCCTACC TAGTGGAGCT GAGCCCAGAC GGCTCCGACT CCAGAGATAA GCCAAAGCTT 
TACCGCCTCC AGTTAAGCGT CACCGAGGTC GGGACGGAGA AGTTCGATGA CAGCTCGATC 
CAGTTGTTTG GTTCCGGAAT CCAGCCCCAC CACTGCGACC TCACCAACAT GGACGGGGTG 
GTCACTGTCA CGCCGAGAAG CGTGGATGCT GAGACCTACG TGGAAGGACA GCGCATCTCG 
GAGACGACCA TGCTGCAGAG CGGGGTGAAG GTGCAGTTCG GGGCGTCCCA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCGCTC GCCAAGAGGC CGGGGGACGG AGGCCTGGCC 
GTCAAGGGTC CCAGACACAA ACCGGGAATC GTTCAGGAGA CAGCTTTCGA TTTAGGAGGA 
GACGTTCACG GGGGGACGGC CGTAGCGAGC AGTAAGGGCT CCGCCCGACC GGACGGCGAC 
AGGGCCCCGT CTGCCTCCAG CAGCGCCGAG CGCGGGATGG TGAAGCCGAT GGTGCGAATG 
GAGCCGCCGC AGGAGCCCCG CAGGCAGGAC ACCAGACCGC AGGACGCCGC TGGGCCTGAG 
CTGACGCTGC CCGCGAGCAT CGAATTCAGG GAAAGTGCTG AAGACTCGTT CTTATCCGCC 
ATCATCAATT ACACGAATAG CTCGACGGTG CATTTTAAGT TGTCGCCTAC GTACGTGCTG 
TACATGGCGT GTCGGCACGT GCTGTCCAGC CAGTACCGCC CGGACGTCAG CCCCGCCGAG 
CGCACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA CGGCGTCATC 
CAGAAACAGA AGAACATCGC GGGGGCGCTT GCGTTCTGGA TGGCCAACGC GTCGGAACTC 
CTCAACTTCA TCAAGCAGGA CCGAGACCTC AGTCGAATCA CACTGGACGC CCAGGATGTT 
TTGGCTCACC TGGTTCAGAT GGCCTTTAAA TACCTGGTTC ACTGTCTTCA GTCAGAGCTG 
AACAACTACA TGCCGGCCTT TCTGGACGAC CCCGAGGAGA ACAGTCTGCA AAGACCAAAA 
ATAGAGGACG TCCTCCACAC GCTCACGGGC GCCATGTCGC TGCTGCGGCG CTGCCGGGTC 
AACGCCGCCC TCACCATCCA GCTCTTCTCC CAGCTCTTCC ACTTCATCAA CATGTGGCTG 
TTCAACAGGC TGCTGACCGA CCCCGAGTCG GGCCTGTGCT CCCACTACTG GGGCGCCATC 
GTCCGCCAGC AGCTGGGCCA CGTGGAGGCC TGGGCGGAGA AGCAGGGCCT GGAGCTGGCT 
GCCGACTGCC ACCTCAGCAG GGTCGTGCAG GCGACCACCC TGCTCACGAT GGACAAGTAC 
GCCCCGGAGG ATGCGGCTGG CATCAACAGC ACCTGCTTCA AGCTGAACTC GCTGCAGCTG 
CAGGCCTTGC TGCACAACTA CCACTGTGCG CCCGACGAGC CCCTGGTCCC GCCGGATCTG 
ATAGAAAGCG TGGTGGCCGT GGCTGAAAAC ACGGCGGACG AGCTGGCGCG CAGCGACGGC 
AGGGAGGTGC GGCTGGAGGA GGACCCTGAC CTGCAGCTGC CCTTCCTGCT GCCGGAGGAC 
GGCTACTCGT GTGACGTCGT CAGAAACATC CCCAACGGCT TGCAGGAGTT TTTGGATCCT 
TTGTGCCAGA GAGGGTTTTG CAGGCTGACG CCCCAGCCGC GCTCTCCGGG TTCCTGGACG 
ATACATTTTG AAGGTGCGGA GTACGAGAGC CGCCTCGCAC GGGAGAGCAC GGAGCTGGCT 
CAGCCTCTGA GGAAAGAACC CGAAGTCATC ACTGTGACCC TGAAGAAGCA GAACGGGATG 
GGCCTCAGCA TCGTCGCAGC GAAGGGCGCA GGTCAAGACA AACTCGGGAT CTACGTCAAG 
TCGGTGGTGA GAGGAGGAGC TGCAGACGTG GACGGGCGGC TGGCGGCGGG GGACCAGCTC 
CTCAGTGTGG ACGGACGAAG CCTGGTGGGC CTCTCTCAGG AAAGGGCAGC AGAGCTGATG 
ACAAGGACAA GTTCCGTGGT GACCCTGGAG GTAGCGAAGC AGGGAGCCAT TTACCACGGC 
CTCGCCACCC TGCTCAACCA GCCCTCGCCC ATGATGCAGA GAATTTCAGA CCGGCGTGGA 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTTGAGCTCT ATAATAACTC AGCTCAGAAC 
GGGTCCCCCG AGAGCCCCCC GCTGCCCTGG GCCGAGTACA GCGAGCCAAA GAAGCTGCCT 
GGCGACGAGA GGCTGACGAA GAACCGCGCC GACCACCGCT CCAGCCCCAA CGTTGCAAAT 
CAGCCTCCTA GTCCTGGTGG GAAAGGTGCA CATGCCGCTG GAACAGCAGC CAAGATCACA 
TCCGTCTCCA CTGGCAACCT CTGCACCGAG GAGCAGACGC CCCCGCCGGG GACGGAGGCC 
TACCCCATCC CCACGCAGGC CTACCCCCGA GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGTCCCCCC CCAGAGCCAG TGGCCAAGCT ACGAGGAGAA GCCGCACGCG 
GACAGCAGCC ACTCCAGCCT CGCGGTTCAG CGGGTGACCC GCTCCCAGGA GGAGCTGCGG 
GAGGACGCGG GGGCCTACCC ACTGCACAGG ATGGAGGCGG TGATGGACCG CAAGTCTGAC 
AGCGACATGT GGGTCGCCCA GAGCTCCTCG ATGGGCTCCA GCGCGTCCAG CCAGGAGCAC 
CTGGACCCCT CTTCCAAGGC GGGCACCCCC GCGTCCACCC TCACCAAGAG CGGCCCGGGG 
CGCTGGAAGA CCCCTGCAGT TCTGCAGCCC GCGCCCGGCC CCCAGCCGCT CCGGACCGAC 
CTGCCCACGC ACTACGCCGG CGACGTGGAC GGCGTCCCCG TGGACCTGCC CCTCCCGCCG 
CCTCCGGCTG CCGCGGGCCA GGGACTCGCG GGGGGCCAAG GGGCCGCCGA GCGGAAGAAG 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCGC GCCTGGAGGA GGAGCGTGAG 
CGCAAGCGGC GCGAGCAGGA GCGCAAGCTG GGCCAGATGC GCTCGCAGGC GCTGCTCCCC 
GCCCCGCTCT CCCCCGGCGC CACCAAGCCC GCCGCCCCGG GGCCCCAGGA CACCGTCATC 
CGGGAGCTGC AGCCTCAGCA GCAGCCGCGC ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACCGTCAGCC GGGAGGAGCT CTCGGCCGGC GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GACGCGCGGG AGAAGCTGGA GAAGCAGCAG CAGATGCACG TGGTGGACCT GCTGAGCCGG 
GAGATTCACG AGCTGCAGAG CAAGGCCGAG CGCTCGGCGG AGGAGAGCGG CCGCCTGCGC 
AAGCTCATGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGACG AGGAGGAGGA CGACGACGTG GACACCATGC TGATCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGTT GCAGGACGAG GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG 
ATGCGGCAGC GGGAGGCGGA GGAGCGTGCC CGCCACGAGG AGGAGCGGCG GCGCCAGGAG 
GAGGAGCGGC TGAAGCGCGA GGCCGAGGAA AAGAGGCGCC AGGAGGAGGG GTACTACAGC 
CGCCTGGAGG CCGAGCGGCG CAGACAGCAC GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC 
GAGGAGCCGG GCCTGGGCCG CCCCCCGCTC CCGCGGGGCT ACGAGCCCCC CGGCCCCGCG 
CCGCCGCCCC CGCCCCAGCG CGCCGCCTCC TACCTGCAGG CCCAGGTCCT GTCCCCCGAC 
TCCCTGTACA CCGCCAAGTT CGTGGCTTAC GACGACGATG ACGAGGAGGA GGACGGCCCT 
GCAGGTCAGG ATAAGTACGC CCGCGCCAGG GGGGCCCGCG GGGGCCCGCG CCCGGCCTCC 
GATGGCATCT TTCTCGGCAG CTCCGCCCAG GCGCCCTGGG CCAGGGCCAA CAGTCCTGCG 
CACAGGGCAG GCGCGCCCCC GGCCCCGCCG CCCAAGCCCG GCCCCGCCCG AGGCCGAGGG 
CCCGCCTCGT ACATGGGATC TACGGGGACG CTGATGGGCG CCCACGATGG CTCTCGGGAC 
CCAAGAGAGA CGCTGTCCAA AAGCCAGGAC GCGGACGTGC CGGGCGGGCC TGAGAGCCTG 
ACCTTCCGGG AGCGCCAGCG CCTCTTCTCC CAGGGCCACG AGGTCTCCAG TAAGGTGAAA 
GCCTCCCGCC GGCTGACGGA GCTGGAGAAC GAGCTGAACA CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839758.1
CDS	22..5409
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKRRQEEGYYSRLEAERRRQHEEAERRLLEPEEPGLGRPPLPRGYEPPGPAPPPPPQRAASYLQAQVLSPDSLYTAKFVAYDDDDEEEDGPAGQDKYARARGARGGPRPASDGIFLGSSAQAPWARANSPAHRAGAPPAPPPKPGPARGRGPASYMGSTGTLMGAHDGSRDPRETLSKSQDADVPGGPESLTFRERQRLFSQGHEVSSKVKASRRLTELENELNTK

Target ORF information:

RefSeq Version	XM_013984304.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984304.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGGC TCAAAGTAAC 
GGACCTGAAA AGCAGGAAAA AGAAGGCGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG 
AAAGAAAAGA AGGAAAAAAA GAAGAGAGAG AAAGAGGCGA TGAGACAAGC ATCTGATAAA 
GATGATAGAC CCTTCCACGG GGAGGACACT GAGAATTCTC GACTGGCTGC CGAGGTTTAC 
AAAGACATGC CCGAAACTAG CTTTACCCGA ACAATCTCCA ACCCCGAGGT GGTCATGAAG 
CGAAGGCGGC AGCAAAAACT GGAAAAGAGG ATGCAGGAGT TTCGGAGCTC AGACGGGCGC 
CCTGATTCCG GTGGAACGTT GAGAATTTAT GCGGATAGTT TAAAGCCAAA TATTCCCTAC 
AAGACAATCC TGCTGTCCAC CACGGACCCC GCCGACTTTG CCGTGGCGGA GGCTTTGGAG 
AAGTACGGTC TGGAGAAGGA GAACCCGAAG GATTACTGCA TCGCCCGGGT TATGCTTCCT 
CCTGGAGCCC AGCATTCGGA TGAGAAAGGT GCTAAAGAAA TTATCCTGGA CGATGACGAG 
TGTCCTTTAC AGATCTTCAG GGAATGGCCT GGTGACAAAG GGATGCTGGT CTTCCAGCTG 
AAGAGGAGGC CCCCAGAGCA CATCCCCAAG AAGAGCAAGA AGCACGCGGA GGGGAAGCCG 
CCCAAGGGGA AGGAGCGGCC CGACGGCTCT GGCTATGGCT CTGCGCTCCC CCCGGAGAAG 
CTGCCCTACC TAGTGGAGCT GAGCCCAGAC GGCTCCGACT CCAGAGATAA GCCAAAGCTT 
TACCGCCTCC AGTTAAGCGT CACCGAGGTC GGGACGGAGA AGTTCGATGA CAGCTCGATC 
CAGTTGTTTG GTTCCGGAAT CCAGCCCCAC CACTGCGACC TCACCAACAT GGACGGGGTG 
GTCACTGTCA CGCCGAGAAG CGTGGATGCT GAGACCTACG TGGAAGGACA GCGCATCTCG 
GAGACGACCA TGCTGCAGAG CGGGGTGAAG GTGCAGTTCG GGGCGTCCCA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCGCTC GCCAAGAGGC CGGGGGACGG AGGCCTGGCC 
GTCAAGGGTC CCAGACACAA ACCGGGAATC GTTCAGGAGA CAGCTTTCGA TTTAGGAGGA 
GACGTTCACG GGGGGACGGC CGTAGCGAGC AGTAAGGGCT CCGCCCGACC GGACGGCGAC 
AGGGCCCCGT CTGCCTCCAG CAGCGCCGAG CGCGGGATGG TGAAGCCGAT GGTGCGAATG 
GAGCCGCCGC AGGAGCCCCG CAGGCAGGAC ACCAGACCGC AGGACGCCGC TGGGCCTGAG 
CTGACGCTGC CCGCGAGCAT CGAATTCAGG GAAAGTGCTG AAGACTCGTT CTTATCCGCC 
ATCATCAATT ACACGAATAG CTCGACGGTG CATTTTAAGT TGTCGCCTAC GTACGTGCTG 
TACATGGCGT GTCGGCACGT GCTGTCCAGC CAGTACCGCC CGGACGTCAG CCCCGCCGAG 
CGCACGCACA AGGTCATCGC CATCGTCAAC AAGATGGTGA GCATGATGGA CGGCGTCATC 
CAGAAACAGA AGAACATCGC GGGGGCGCTT GCGTTCTGGA TGGCCAACGC GTCGGAACTC 
CTCAACTTCA TCAAGCAGGA CCGAGACCTC AGTCGAATCA CACTGGACGC CCAGGATGTT 
TTGGCTCACC TGGTTCAGAT GGCCTTTAAA TACCTGGTTC ACTGTCTTCA GTCAGAGCTG 
AACAACTACA TGCCGGCCTT TCTGGACGAC CCCGAGGAGA ACAGTCTGCA AAGACCAAAA 
ATAGAGGACG TCCTCCACAC GCTCACGGGC GCCATGTCGC TGCTGCGGCG CTGCCGGGTC 
AACGCCGCCC TCACCATCCA GCTCTTCTCC CAGCTCTTCC ACTTCATCAA CATGTGGCTG 
TTCAACAGGC TGCTGACCGA CCCCGAGTCG GGCCTGTGCT CCCACTACTG GGGCGCCATC 
GTCCGCCAGC AGCTGGGCCA CGTGGAGGCC TGGGCGGAGA AGCAGGGCCT GGAGCTGGCT 
GCCGACTGCC ACCTCAGCAG GGTCGTGCAG GCGACCACCC TGCTCACGAT GGACAAGTAC 
GCCCCGGAGG ATGCGGCTGG CATCAACAGC ACCTGCTTCA AGCTGAACTC GCTGCAGCTG 
CAGGCCTTGC TGCACAACTA CCACTGTGCG CCCGACGAGC CCCTGGTCCC GCCGGATCTG 
ATAGAAAGCG TGGTGGCCGT GGCTGAAAAC ACGGCGGACG AGCTGGCGCG CAGCGACGGC 
AGGGAGGTGC GGCTGGAGGA GGACCCTGAC CTGCAGCTGC CCTTCCTGCT GCCGGAGGAC 
GGCTACTCGT GTGACGTCGT CAGAAACATC CCCAACGGCT TGCAGGAGTT TTTGGATCCT 
TTGTGCCAGA GAGGGTTTTG CAGGCTGACG CCCCAGCCGC GCTCTCCGGG TTCCTGGACG 
ATACATTTTG AAGGTGCGGA GTACGAGAGC CGCCTCGCAC GGGAGAGCAC GGAGCTGGCT 
CAGCCTCTGA GGAAAGAACC CGAAGTCATC ACTGTGACCC TGAAGAAGCA GAACGGGATG 
GGCCTCAGCA TCGTCGCAGC GAAGGGCGCA GGTCAAGACA AACTCGGGAT CTACGTCAAG 
TCGGTGGTGA GAGGAGGAGC TGCAGACGTG GACGGGCGGC TGGCGGCGGG GGACCAGCTC 
CTCAGTGTGG ACGGACGAAG CCTGGTGGGC CTCTCTCAGG AAAGGGCAGC AGAGCTGATG 
ACAAGGACAA GTTCCGTGGT GACCCTGGAG GTAGCGAAGC AGGGAGCCAT TTACCACGGC 
CTCGCCACCC TGCTCAACCA GCCCTCGCCC ATGATGCAGA GAATTTCAGA CCGGCGTGGA 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTTGAGCTCT ATAATAACTC AGCTCAGAAC 
GGGTCCCCCG AGAGCCCCCC GCTGCCCTGG GCCGAGTACA GCGAGCCAAA GAAGCTGCCT 
GGCGACGAGA GGCTGACGAA GAACCGCGCC GACCACCGCT CCAGCCCCAA CGTTGCAAAT 
CAGCCTCCTA GTCCTGGTGG GAAAGGTGCA CATGCCGCTG GAACAGCAGC CAAGATCACA 
TCCGTCTCCA CTGGCAACCT CTGCACCGAG GAGCAGACGC CCCCGCCGGG GACGGAGGCC 
TACCCCATCC CCACGCAGGC CTACCCCCGA GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGTCCCCCC CCAGAGCCAG TGGCCAAGCT ACGAGGAGAA GCCGCACGCG 
GACAGCAGCC ACTCCAGCCT CGCGGTTCAG CGGGTGACCC GCTCCCAGGA GGAGCTGCGG 
GAGGACGCGG GGGCCTACCC ACTGCACAGG ATGGAGGCGG TGATGGACCG CAAGTCTGAC 
AGCGACATGT GGGTCGCCCA GAGCTCCTCG ATGGGCTCCA GCGCGTCCAG CCAGGAGCAC 
CTGGACCCCT CTTCCAAGGC GGGCACCCCC GCGTCCACCC TCACCAAGAG CGGCCCGGGG 
CGCTGGAAGA CCCCTGCAGT TCTGCAGCCC GCGCCCGGCC CCCAGCCGCT CCGGACCGAC 
CTGCCCACGC ACTACGCCGG CGACGTGGAC GGCGTCCCCG TGGACCTGCC CCTCCCGCCG 
CCTCCGGCTG CCGCGGGCCA GGGACTCGCG GGGGGCCAAG GGGCCGCCGA GCGGAAGAAG 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCGC GCCTGGAGGA GGAGCGTGAG 
CGCAAGCGGC GCGAGCAGGA GCGCAAGCTG GGCCAGATGC GCTCGCAGGC GCTGCTCCCC 
GCCCCGCTCT CCCCCGGCGC CACCAAGCCC GCCGCCCCGG GGCCCCAGGA CACCGTCATC 
CGGGAGCTGC AGCCTCAGCA GCAGCCGCGC ACCATCGAGC GCAGGGACCT GCAGTACATC 
ACCGTCAGCC GGGAGGAGCT CTCGGCCGGC GACAGCCTGT CCCCCGACCC CTGGAAGCGC 
GACGCGCGGG AGAAGCTGGA GAAGCAGCAG CAGATGCACG TGGTGGACCT GCTGAGCCGG 
GAGATTCACG AGCTGCAGAG CAAGGCCGAG CGCTCGGCGG AGGAGAGCGG CCGCCTGCGC 
AAGCTCATGC TGGAGTGGCA GTTCCAGAAG CGGCTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGACG AGGAGGAGGA CGACGACGTG GACACCATGC TGATCATGCA GCGCCTGGAG 
GCCGAGCGCA GAGCGCGGTT GCAGGACGAG GAGCGGCGGC GGCAGCAGCA GCTGGAGGAG 
ATGCGGCAGC GGGAGGCGGA GGAGCGTGCC CGCCACGAGG AGGAGCGGCG GCGCCAGGAG 
GAGGAGCGGC TGAAGCGCGA GGCCGAGGAA AAGAGGCGCC AGGAGGAGGG GTACTACAGC 
CGCCTGGAGG CCGAGCGGCG CAGACAGCAC GAGGAGGCCG AGCGCCGGCT GCTGGAGCCC 
GAGGAGCCGG GCCTGGGCCG CCCCCCGCTC CCGCGGGGCT ACGAGCCCCC CGGCCCCGCG 
CCGCCGCCCC CGCCCCAGCG CGCCGCCTCC TACCTGCAGG CCCAGGTCCT GTCCCCCGAC 
TCCCTGTACA CCGCCAAGTT CGTGGCTTAC GACGACGATG ACGAGGAGGA GGACGGCCCT 
GCAGGTCAGG ATAAGTACGC CCGCGCCAGG GGGGCCCGCG GGGGCCCGCG CCCGGCCTCC 
GATGGCATCT TTCTCGGCAG CTCCGCCCAG GCGCCCTGGG CCAGGGCCAA CAGTCCTGCG 
CACAGGGCAG GCGCGCCCCC GGCCCCGCCG CCCAAGCCCG GCCCCGCCCG AGGCCGAGGG 
CCCGCCTCGT ACATGGGATC TACGGGGACG CTGATGGGCG CCCACGATGG CTCTCGGGAC 
CCAAGAGAGA CGCTGTCCAA AAGCCAGGAC GCGGACGTGC CGGGCGGGCC TGAGAGCCTG 
ACCTTCCGGG AGCGCCAGCG CCTCTTCTCC CAGGGCCACG AGGTCTCCAG TAAGGTGAAA 
GCCTCCCGCC GGCTGACGGA GCTGGAGAAC GAGCTGAACA CCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_013984306.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013839760.1 Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA.

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CloneID	OSe30618
Clone ID Related Accession (Same CDS sequence)	XM_013984306.1
Accession Version	XM_013984306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	afadin isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003533884.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GAAACAGAAG 
AACATCGCGG GGGCGCTTGC GTTCTGGATG GCCAACGCGT CGGAACTCCT CAACTTCATC 
AAGCAGGACC GAGACCTCAG TCGAATCACA CTGGACGCCC AGGATGTTTT GGCTCACCTG 
GTTCAGATGG CCTTTAAATA CCTGGTTCAC TGTCTTCAGT CAGAGCTGAA CAACTACATG 
CCGGCCTTTC TGGACGACCC CGAGGAGAAC AGTCTGCAAA GACCAAAAAT AGAGGACGTC 
CTCCACACGC TCACGGGCGC CATGTCGCTG CTGCGGCGCT GCCGGGTCAA CGCCGCCCTC 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCATCAACA TGTGGCTGTT CAACAGGCTG 
CTGACCGACC CCGAGTCGGG CCTGTGCTCC CACTACTGGG GCGCCATCGT CCGCCAGCAG 
CTGGGCCACG TGGAGGCCTG GGCGGAGAAG CAGGGCCTGG AGCTGGCTGC CGACTGCCAC 
CTCAGCAGGG TCGTGCAGGC GACCACCCTG CTCACGATGG ACAAGTACGC CCCGGAGGAT 
GCGGCTGGCA TCAACAGCAC CTGCTTCAAG CTGAACTCGC TGCAGCTGCA GGCCTTGCTG 
CACAACTACC ACTGTGCGCC CGACGAGCCC CTGGTCCCGC CGGATCTGAT AGAAAGCGTG 
GTGGCCGTGG CTGAAAACAC GGCGGACGAG CTGGCGCGCA GCGACGGCAG GGAGGTGCGG 
CTGGAGGAGG ACCCTGACCT GCAGCTGCCC TTCCTGCTGC CGGAGGACGG CTACTCGTGT 
GACGTCGTCA GAAACATCCC CAACGGCTTG CAGGAGTTTT TGGATCCTTT GTGCCAGAGA 
GGGTTTTGCA GGCTGACGCC CCAGCCGCGC TCTCCGGGTT CCTGGACGAT ACATTTTGAA 
GGTGCGGAGT ACGAGAGCCG CCTCGCACGG GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCCG AAGTCATCAC TGTGACCCTG AAGAAGCAGA ACGGGATGGG CCTCAGCATC 
GTCGCAGCGA AGGGCGCAGG TCAAGACAAA CTCGGGATCT ACGTCAAGTC GGTGGTGAGA 
GGAGGAGCTG CAGACGTGGA CGGGCGGCTG GCGGCGGGGG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGCC TGGTGGGCCT CTCTCAGGAA AGGGCAGCAG AGCTGATGAC AAGGACAAGT 
TCCGTGGTGA CCCTGGAGGT AGCGAAGCAG GGAGCCATTT ACCACGGCCT CGCCACCCTG 
CTCAACCAGC CCTCGCCCAT GATGCAGAGA ATTTCAGACC GGCGTGGATC AGGTAAACCC 
CGACCAAAGA GCGAAGGTTT TGAGCTCTAT AATAACTCAG CTCAGAACGG GTCCCCCGAG 
AGCCCCCCGC TGCCCTGGGC CGAGTACAGC GAGCCAAAGA AGCTGCCTGG CGACGAGAGG 
CTGACGAAGA ACCGCGCCGA CCACCGCTCC AGCCCCAACG TTGCAAATCA GCCTCCTAGT 
CCTGGTGGGA AAGGTGCACA TGCCGCTGGA ACAGCAGCCA AGATCACATC CGTCTCCACT 
GGCAACCTCT GCACCGAGGA GCAGACGCCC CCGCCGGGGA CGGAGGCCTA CCCCATCCCC 
ACGCAGGCCT ACCCCCGAGA GTACTTCACC TTCCCGGCCT CCAGGTCCCA GGACCGCGTG 
GTCCCCCCCC AGAGCCAGTG GCCAAGCTAC GAGGAGAAGC CGCACGCGGA CAGCAGCCAC 
TCCAGCCTCG CGGTTCAGCG GGTGACCCGC TCCCAGGAGG AGCTGCGGGA GGACGCGGGG 
GCCTACCCAC TGCACAGGAT GGAGGCGGTG ATGGACCGCA AGTCTGACAG CGACATGTGG 
GTCGCCCAGA GCTCCTCGAT GGGCTCCAGC GCGTCCAGCC AGGAGCACCT GGACCCCTCT 
TCCAAGGCGG GCACCCCCGC GTCCACCCTC ACCAAGAGCG GCCCGGGGCG CTGGAAGACC 
CCTGCAGTTC TGCAGCCCGC GCCCGGCCCC CAGCCGCTCC GGACCGACCT GCCCACGCAC 
TACGCCGGCG ACGTGGACGG CGTCCCCGTG GACCTGCCCC TCCCGCCGCC TCCGGCTGCC 
GCGGGCCAGG GACTCGCGGG GGGCCAAGGG GCCGCCGAGC GGAAGAAGCG GGAGGAGCAG 
CAGCGCTGGT ACGAGAAGGA GAAGGCGCGC CTGGAGGAGG AGCGTGAGCG CAAGCGGCGC 
GAGCAGGAGC GCAAGCTGGG CCAGATGCGC TCGCAGGCGC TGCTCCCCGC CCCGCTCTCC 
CCCGGCGCCA CCAAGCCCGC CGCCCCGGGG CCCCAGGACA CCGTCATCCG GGAGCTGCAG 
CCTCAGCAGC AGCCGCGCAC CATCGAGCGC AGGGACCTGC AGTACATCAC CGTCAGCCGG 
GAGGAGCTCT CGGCCGGCGA CAGCCTGTCC CCCGACCCCT GGAAGCGCGA CGCGCGGGAG 
AAGCTGGAGA AGCAGCAGCA GATGCACGTG GTGGACCTGC TGAGCCGGGA GATTCACGAG 
CTGCAGAGCA AGGCCGAGCG CTCGGCGGAG GAGAGCGGCC GCCTGCGCAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGACGAG 
GAGGAGGACG ACGACGTGGA CACCATGCTG ATCATGCAGC GCCTGGAGGC CGAGCGCAGA 
GCGCGGGACG AGGAGCGGCG GCGGCAGCAG CAGCTGGAGG AGATGCGGCA GCGGGAGGCG 
GAGGAGCGTG CCCGCCACGA GGAGGAGCGG CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC 
GAGGCCGAGG AAAAGGTCAT GGTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013839760.1
CDS	22..4791
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAAPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAMRQASDKDDRPFHGEDTENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPGDKGMLVFQLKRRPPEHIPKKSKKHAEGKPPKGKERPDGSGYGSALPPEKLPYLVELSPGRRGHFAYYSYHPHEDGSDSRDKPKLYRLQLSVTEVGTEKFDDSSIQLFGSGIQPHHCDLTNMDGVVTVTPRSVDAETYVEGQRISETTMLQSGVKVQFGASHVFKFVDPSQDHALAKRPGDGGLAVKGPRHKPGIVQETAFDLGGDVHGGTAVASSKGSARPDGDRAPSASSSAERGMVKPMVRMEPPQEPRRQDTRPQDAAGPELTLPASIEFRESAEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRHVLSSQYRPDVSPAERTHKVIAIVNKMVSMMDGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIEDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLLTDPESGLCSHYWGAIVRQQLGHVEAWAEKQGLELAADCHLSRVVQATTLLTMDKYAPEDAAGINSTCFKLNSLQLQALLHNYHCAPDEPLVPPDLIESVVAVAENTADELARSDGREVRLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLTPQPRSPGSWTIHFEGAEYESRLARESTELAQPLRKEPEVITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVRGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAQNGSPESPPLPWAEYSEPKKLPGDERLTKNRADHRSSPNVANQPPSPGGKGAHAAGTAAKITSVSTGNLCTEEQTPPPGTEAYPIPTQAYPREYFTFPASRSQDRVVPPQSQWPSYEEKPHADSSHSSLAVQRVTRSQEELREDAGAYPLHRMEAVMDRKSDSDMWVAQSSSMGSSASSQEHLDPSSKAGTPASTLTKSGPGRWKTPAVLQPAPGPQPLRTDLPTHYAGDVDGVPVDLPLPPPPAAAGQGLAGGQGAAERKKREEQQRWYEKEKARLEEERERKRREQERKLGQMRSQALLPAPLSPGATKPAAPGPQDTVIRELQPQQQPRTIERRDLQYITVSREELSAGDSLSPDPWKRDAREKLEKQQQMHVVDLLSREIHELQSKAERSAEESGRLRKLMLEWQFQKRLQESKQKDEDDDEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRQREAEERARHEEERRRQEEERLKREAEEKVMVL

Target ORF information:

RefSeq Version	XM_013984306.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013984306.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACTG CCACCACTCA GGATGTGATC GAGACGCTGG CCGAGAAGTT CCGGCCGGAT 
ATGCGGATGC TCTCGTCCCC CAAGTACTCG CTCTACGAGG TGCACGTCAG CGGAGAAAGA 
AGATTGGATA TAGACGAGAA GCCCCTAGTT GTGCAGCTGA ATTGGAATAA AGATGACCGC 
GAGGGCAGGT TTGTCCTTAA GAACGAGAAC GACGCCGCTC CTCCCAAGAA GGCTCAAAGT 
AACGGACCTG AAAAGCAGGA AAAAGAAGGC GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAAA AGAAGGAAAA AAAGAAGAGA GAGAAAGAGG CGATGAGACA AGCATCTGAT 
AAAGATGATA GACCCTTCCA CGGGGAGGAC ACTGAGAATT CTCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCCGAAAC TAGCTTTACC CGAACAATCT CCAACCCCGA GGTGGTCATG 
AAGCGAAGGC GGCAGCAAAA ACTGGAAAAG AGGATGCAGG AGTTTCGGAG CTCAGACGGG 
CGCCCTGATT CCGGTGGAAC GTTGAGAATT TATGCGGATA GTTTAAAGCC AAATATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAC CCCGCCGACT TTGCCGTGGC GGAGGCTTTG 
GAGAAGTACG GTCTGGAGAA GGAGAACCCG AAGGATTACT GCATCGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC GGATGAGAAA GGTGCTAAAG AAATTATCCT GGACGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCTGGTGACA AAGGGATGCT GGTCTTCCAG 
CTGAAGAGGA GGCCCCCAGA GCACATCCCC AAGAAGAGCA AGAAGCACGC GGAGGGGAAG 
CCGCCCAAGG GGAAGGAGCG GCCCGACGGC TCTGGCTATG GCTCTGCGCT CCCCCCGGAG 
AAGCTGCCCT ACCTAGTGGA GCTGAGCCCA GGGAGAAGGG GTCACTTTGC CTACTACAGC 
TATCACCCTC ACGAAGACGG CTCCGACTCC AGAGATAAGC CAAAGCTTTA CCGCCTCCAG 
TTAAGCGTCA CCGAGGTCGG GACGGAGAAG TTCGATGACA GCTCGATCCA GTTGTTTGGT 
TCCGGAATCC AGCCCCACCA CTGCGACCTC ACCAACATGG ACGGGGTGGT CACTGTCACG 
CCGAGAAGCG TGGATGCTGA GACCTACGTG GAAGGACAGC GCATCTCGGA GACGACCATG 
CTGCAGAGCG GGGTGAAGGT GCAGTTCGGG GCGTCCCACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCGCTCGC CAAGAGGCCG GGGGACGGAG GCCTGGCCGT CAAGGGTCCC 
AGACACAAAC CGGGAATCGT TCAGGAGACA GCTTTCGATT TAGGAGGAGA CGTTCACGGG 
GGGACGGCCG TAGCGAGCAG TAAGGGCTCC GCCCGACCGG ACGGCGACAG GGCCCCGTCT 
GCCTCCAGCA GCGCCGAGCG CGGGATGGTG AAGCCGATGG TGCGAATGGA GCCGCCGCAG 
GAGCCCCGCA GGCAGGACAC CAGACCGCAG GACGCCGCTG GGCCTGAGCT GACGCTGCCC 
GCGAGCATCG AATTCAGGGA AAGTGCTGAA GACTCGTTCT TATCCGCCAT CATCAATTAC 
ACGAATAGCT CGACGGTGCA TTTTAAGTTG TCGCCTACGT ACGTGCTGTA CATGGCGTGT 
CGGCACGTGC TGTCCAGCCA GTACCGCCCG GACGTCAGCC CCGCCGAGCG CACGCACAAG 
GTCATCGCCA TCGTCAACAA GATGGTGAGC ATGATGGACG GCGTCATCCA GAAACAGAAG 
AACATCGCGG GGGCGCTTGC GTTCTGGATG GCCAACGCGT CGGAACTCCT CAACTTCATC 
AAGCAGGACC GAGACCTCAG TCGAATCACA CTGGACGCCC AGGATGTTTT GGCTCACCTG 
GTTCAGATGG CCTTTAAATA CCTGGTTCAC TGTCTTCAGT CAGAGCTGAA CAACTACATG 
CCGGCCTTTC TGGACGACCC CGAGGAGAAC AGTCTGCAAA GACCAAAAAT AGAGGACGTC 
CTCCACACGC TCACGGGCGC CATGTCGCTG CTGCGGCGCT GCCGGGTCAA CGCCGCCCTC 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCATCAACA TGTGGCTGTT CAACAGGCTG 
CTGACCGACC CCGAGTCGGG CCTGTGCTCC CACTACTGGG GCGCCATCGT CCGCCAGCAG 
CTGGGCCACG TGGAGGCCTG GGCGGAGAAG CAGGGCCTGG AGCTGGCTGC CGACTGCCAC 
CTCAGCAGGG TCGTGCAGGC GACCACCCTG CTCACGATGG ACAAGTACGC CCCGGAGGAT 
GCGGCTGGCA TCAACAGCAC CTGCTTCAAG CTGAACTCGC TGCAGCTGCA GGCCTTGCTG 
CACAACTACC ACTGTGCGCC CGACGAGCCC CTGGTCCCGC CGGATCTGAT AGAAAGCGTG 
GTGGCCGTGG CTGAAAACAC GGCGGACGAG CTGGCGCGCA GCGACGGCAG GGAGGTGCGG 
CTGGAGGAGG ACCCTGACCT GCAGCTGCCC TTCCTGCTGC CGGAGGACGG CTACTCGTGT 
GACGTCGTCA GAAACATCCC CAACGGCTTG CAGGAGTTTT TGGATCCTTT GTGCCAGAGA 
GGGTTTTGCA GGCTGACGCC CCAGCCGCGC TCTCCGGGTT CCTGGACGAT ACATTTTGAA 
GGTGCGGAGT ACGAGAGCCG CCTCGCACGG GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCCG AAGTCATCAC TGTGACCCTG AAGAAGCAGA ACGGGATGGG CCTCAGCATC 
GTCGCAGCGA AGGGCGCAGG TCAAGACAAA CTCGGGATCT ACGTCAAGTC GGTGGTGAGA 
GGAGGAGCTG CAGACGTGGA CGGGCGGCTG GCGGCGGGGG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGCC TGGTGGGCCT CTCTCAGGAA AGGGCAGCAG AGCTGATGAC AAGGACAAGT 
TCCGTGGTGA CCCTGGAGGT AGCGAAGCAG GGAGCCATTT ACCACGGCCT CGCCACCCTG 
CTCAACCAGC CCTCGCCCAT GATGCAGAGA ATTTCAGACC GGCGTGGATC AGGTAAACCC 
CGACCAAAGA GCGAAGGTTT TGAGCTCTAT AATAACTCAG CTCAGAACGG GTCCCCCGAG 
AGCCCCCCGC TGCCCTGGGC CGAGTACAGC GAGCCAAAGA AGCTGCCTGG CGACGAGAGG 
CTGACGAAGA ACCGCGCCGA CCACCGCTCC AGCCCCAACG TTGCAAATCA GCCTCCTAGT 
CCTGGTGGGA AAGGTGCACA TGCCGCTGGA ACAGCAGCCA AGATCACATC CGTCTCCACT 
GGCAACCTCT GCACCGAGGA GCAGACGCCC CCGCCGGGGA CGGAGGCCTA CCCCATCCCC 
ACGCAGGCCT ACCCCCGAGA GTACTTCACC TTCCCGGCCT CCAGGTCCCA GGACCGCGTG 
GTCCCCCCCC AGAGCCAGTG GCCAAGCTAC GAGGAGAAGC CGCACGCGGA CAGCAGCCAC 
TCCAGCCTCG CGGTTCAGCG GGTGACCCGC TCCCAGGAGG AGCTGCGGGA GGACGCGGGG 
GCCTACCCAC TGCACAGGAT GGAGGCGGTG ATGGACCGCA AGTCTGACAG CGACATGTGG 
GTCGCCCAGA GCTCCTCGAT GGGCTCCAGC GCGTCCAGCC AGGAGCACCT GGACCCCTCT 
TCCAAGGCGG GCACCCCCGC GTCCACCCTC ACCAAGAGCG GCCCGGGGCG CTGGAAGACC 
CCTGCAGTTC TGCAGCCCGC GCCCGGCCCC CAGCCGCTCC GGACCGACCT GCCCACGCAC 
TACGCCGGCG ACGTGGACGG CGTCCCCGTG GACCTGCCCC TCCCGCCGCC TCCGGCTGCC 
GCGGGCCAGG GACTCGCGGG GGGCCAAGGG GCCGCCGAGC GGAAGAAGCG GGAGGAGCAG 
CAGCGCTGGT ACGAGAAGGA GAAGGCGCGC CTGGAGGAGG AGCGTGAGCG CAAGCGGCGC 
GAGCAGGAGC GCAAGCTGGG CCAGATGCGC TCGCAGGCGC TGCTCCCCGC CCCGCTCTCC 
CCCGGCGCCA CCAAGCCCGC CGCCCCGGGG CCCCAGGACA CCGTCATCCG GGAGCTGCAG 
CCTCAGCAGC AGCCGCGCAC CATCGAGCGC AGGGACCTGC AGTACATCAC CGTCAGCCGG 
GAGGAGCTCT CGGCCGGCGA CAGCCTGTCC CCCGACCCCT GGAAGCGCGA CGCGCGGGAG 
AAGCTGGAGA AGCAGCAGCA GATGCACGTG GTGGACCTGC TGAGCCGGGA GATTCACGAG 
CTGCAGAGCA AGGCCGAGCG CTCGGCGGAG GAGAGCGGCC GCCTGCGCAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGCG GCTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGACGAG 
GAGGAGGACG ACGACGTGGA CACCATGCTG ATCATGCAGC GCCTGGAGGC CGAGCGCAGA 
GCGCGGGACG AGGAGCGGCG GCGGCAGCAG CAGCTGGAGG AGATGCGGCA GCGGGAGGCG 
GAGGAGCGTG CCCGCCACGA GGAGGAGCGG CGGCGCCAGG AGGAGGAGCG GCTGAAGCGC 
GAGGCCGAGG AAAAGGTCAT GGTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AFDN
Entrez Gene ID	100514741
Full Name	afadin, adherens junction formation factor
Gene Type	protein-coding
Organism	Sus scrofa(pig)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AFDN cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

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