MDK cDNA ORF clone, Sus scrofa(Pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	MDK
Entrez Gene ID	100359358
Full Name	midkine
Gene Type	protein-coding
Organism	Sus scrofa(Pig)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005660939.2	XP_005660996.1	midkine isoform X1
XM_005660939.3	XP_005660996.1	midkine isoform X1
NM_001195352.1	NP_001182281.1	midkine precursor
XM_005660938.2	XP_005660995.1	midkine isoform X1
XM_005660938.3	XP_005660995.1	midkine isoform X1
XM_005660940.2	XP_005660997.1	midkine isoform X2
XM_005660940.1	XP_005660997.1	midkine isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Midkine promotes proliferation of primordial germ cells by inhibiting the expression of the deleted in azoospermia-like gene.
Shen W, Park BW, Toms D, Li J
Endocrinology153(7)3482-92(2012 Jul)

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D, Hamasima N, Awata T
Nucleic acids research35(Database issue)D650-3(2007 Jan)

Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.
Jørgensen FG, Hobolth A, Hornshøj H, Bendixen C, Fredholm M, Schierup MH
BMC biology32(2005 Jan)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MDK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe04413	NM_001195352.1 Latest version!	Sus scrofa midkine (MDK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OSe04412	XM_005660938.3 Latest version!	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OSe04412	XM_005660939.3 Latest version!	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OSe04413	XM_005660940.2 Latest version!	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OSe04412	XM_005660939.2	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OSe04412	XM_005660938.2	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OSe04413	XM_005660940.1	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDK ( NM_001195352.1 ) cDNA ORF clone, Sus scrofa(Pig) -> NP_001182281.1 Sus scrofa midkine (MDK), mRNA.

Price & Availability↑

CloneID	OSe04413
Clone ID Related Accession (Same CDS sequence)	NM_001195352.1 , XM_005660940.2 , XM_005660940.1
Accession Version	NM_001195352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1501948800000
Organism	Sus scrofa(pig)
Product	midkine precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU373711.1. ##Evidence-Data-START## Transcript exon combination :: GU373711.1, CK458616.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02584787, SAMN02584789 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCCAA GAAAGGGAAG 
GGAAAAGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182281.1
CDS	63..494
Translation	MQRRGFLLLALFALLALTDAVAKKKDKVKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRVRCRVPCNWKKEFGADCKYKFESWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCTPKTKAKAKAKKGKGKD

Target ORF information:

RefSeq Version	NM_001195352.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa midkine (MDK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195352.1

ORF Insert Sequence:

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCCAA GAAAGGGAAG 
GGAAAAGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005660939.2 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_005660996.1 Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe04412
Clone ID Related Accession (Same CDS sequence)	XM_005660939.2 , XM_005660939.3 , XM_005660938.2 , XM_005660938.3
Accession Version	XM_005660939.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	midkine isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005660939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCTTC ATTTCTGACT 
CACAGCAGCC CTGGAGGCCA ACAGGGTAGA GGTGGATCTG CCCACTTCTC AGCCAAGAAA 
GGGAAGGGAA AAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005660996.1
CDS	221..718
Translation	MQRRGFLLLALFALLALTDAVAKKKDKVKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRVRCRVPCNWKKEFGADCKYKFESWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCTPKTKAKAKASFLTHSSPGGQQGRGGSAHFSAKKGKGKD

Target ORF information:

RefSeq Version	XM_005660939.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005660939.2

ORF Insert Sequence:

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCTTC ATTTCTGACT 
CACAGCAGCC CTGGAGGCCA ACAGGGTAGA GGTGGATCTG CCCACTTCTC AGCCAAGAAA 
GGGAAGGGAA AAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005660939.3 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_005660996.1 Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe04412
Clone ID Related Accession (Same CDS sequence)	XM_005660939.2 , XM_005660939.3 , XM_005660938.2 , XM_005660938.3
Accession Version	XM_005660939.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	midkine isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005660939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCTTC ATTTCTGACT 
CACAGCAGCC CTGGAGGCCA ACAGGGTAGA GGTGGATCTG CCCACTTCTC AGCCAAGAAA 
GGGAAGGGAA AAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005660996.1
CDS	214..711
Translation	MQRRGFLLLALFALLALTDAVAKKKDKVKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRVRCRVPCNWKKEFGADCKYKFESWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCTPKTKAKAKASFLTHSSPGGQQGRGGSAHFSAKKGKGKD

Target ORF information:

RefSeq Version	XM_005660939.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005660939.3

ORF Insert Sequence:

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCTTC ATTTCTGACT 
CACAGCAGCC CTGGAGGCCA ACAGGGTAGA GGTGGATCTG CCCACTTCTC AGCCAAGAAA 
GGGAAGGGAA AAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005660938.2 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_005660995.1 Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe04412
Clone ID Related Accession (Same CDS sequence)	XM_005660939.2 , XM_005660939.3 , XM_005660938.2 , XM_005660938.3
Accession Version	XM_005660938.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	midkine isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609533.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_005660938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCTTC ATTTCTGACT 
CACAGCAGCC CTGGAGGCCA ACAGGGTAGA GGTGGATCTG CCCACTTCTC AGCCAAGAAA 
GGGAAGGGAA AAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005660995.1
CDS	473..970
Translation	MQRRGFLLLALFALLALTDAVAKKKDKVKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRVRCRVPCNWKKEFGADCKYKFESWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCTPKTKAKAKASFLTHSSPGGQQGRGGSAHFSAKKGKGKD

Target ORF information:

RefSeq Version	XM_005660938.2
Organism	Sus scrofa(Pig)
Definition	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005660938.2

ORF Insert Sequence:

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCTTC ATTTCTGACT 
CACAGCAGCC CTGGAGGCCA ACAGGGTAGA GGTGGATCTG CCCACTTCTC AGCCAAGAAA 
GGGAAGGGAA AAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005660938.3 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_005660995.1 Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe04412
Clone ID Related Accession (Same CDS sequence)	XM_005660939.2 , XM_005660939.3 , XM_005660938.2 , XM_005660938.3
Accession Version	XM_005660938.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	midkine isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005660938.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCTTC ATTTCTGACT 
CACAGCAGCC CTGGAGGCCA ACAGGGTAGA GGTGGATCTG CCCACTTCTC AGCCAAGAAA 
GGGAAGGGAA AAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005660995.1
CDS	463..960
Translation	MQRRGFLLLALFALLALTDAVAKKKDKVKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRVRCRVPCNWKKEFGADCKYKFESWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCTPKTKAKAKASFLTHSSPGGQQGRGGSAHFSAKKGKGKD

Target ORF information:

RefSeq Version	XM_005660938.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005660938.3

ORF Insert Sequence:

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCTTC ATTTCTGACT 
CACAGCAGCC CTGGAGGCCA ACAGGGTAGA GGTGGATCTG CCCACTTCTC AGCCAAGAAA 
GGGAAGGGAA AAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005660940.2 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_005660997.1 Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSe04413
Clone ID Related Accession (Same CDS sequence)	NM_001195352.1 , XM_005660940.2 , XM_005660940.1
Accession Version	XM_005660940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1494518400000
Organism	Sus scrofa(pig)
Product	midkine isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010444.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_005660940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCCAA GAAAGGGAAG 
GGAAAAGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005660997.1
CDS	227..658
Translation	MQRRGFLLLALFALLALTDAVAKKKDKVKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRVRCRVPCNWKKEFGADCKYKFESWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCTPKTKAKAKAKKGKGKD

Target ORF information:

RefSeq Version	XM_005660940.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005660940.2

ORF Insert Sequence:

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCCAA GAAAGGGAAG 
GGAAAAGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005660940.1 ) cDNA ORF clone, Sus scrofa(Pig) -> XP_005660997.1 Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X3, mRNA.

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CloneID	OSe04413
Clone ID Related Accession (Same CDS sequence)	NM_001195352.1 , XM_005660940.2 , XM_005660940.1
Accession Version	XM_005660940.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441814400000
Organism	Sus scrofa(Pig)
Product	midkine isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003609533.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCCAA GAAAGGGAAG 
GGAAAAGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005660997.1
CDS	220..651
Translation	MQRRGFLLLALFALLALTDAVAKKKDKVKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRVRCRVPCNWKKEFGADCKYKFESWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCTPKTKAKAKAKKGKGKD

Target ORF information:

RefSeq Version	XM_005660940.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa midkine (neurite growth-promoting factor 2) (MDK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005660940.1

ORF Insert Sequence:

ATGCAGCGCC GAGGCTTCCT CCTCCTCGCC CTCTTCGCCC TGCTAGCGCT CACCGACGCG 
GTGGCCAAAA AGAAAGACAA AGTGAAGAAG GGCGGCCCGG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGAG TGGGTTTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CGTCCGGTGC CGAGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCTGACT GCAAGTACAA GTTTGAGAGC TGGGGGGCAT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGCACCCT GAAGAAGGCT CGGTACAATG CTCAGTGCCA GGAGACCATC 
CGTGTGACCA AGCCCTGCAC CCCCAAGACC AAAGCCAAGG CCAAAGCCAA GAAAGGGAAG 
GGAAAAGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Midkine promotes proliferation of primordial germ cells by inhibiting the expression of the deleted in azoospermia-like gene.
Endocrinology153(7)3482-92(2012 Jul)
Shen W,Park BW,Toms D,Li J

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Nucleic acids research35(Database issue)D650-3(2007 Jan)
Uenishi H,Eguchi-Ogawa T,Shinkai H,Okumura N,Suzuki K,Toki D,Hamasima N,Awata T

Comparative analysis of protein coding sequences from human, mouse and the domesticated pig.
BMC biology32(2005 Jan)
Jørgensen FG,Hobolth A,Hornshøj H,Bendixen C,Fredholm M,Schierup MH

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T

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MDK cDNA ORF clone, Sus scrofa(Pig)

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ORF Insert Sequence:

Target ORF information:

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